Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24389.t1 | AES59462.1 | 95.7 | 93 | 4 | 0 | 1 | 93 | 1 | 93 | 2.20E-43 | 184.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24389.t1 | G7I3M7 | 95.7 | 93 | 4 | 0 | 1 | 93 | 1 | 93 | 1.6e-43 | 184.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050750 | MS.gene24389 | 0.800672 | 1.29E-48 | -1.69E-46 |
| MS.gene050953 | MS.gene24389 | 0.800571 | 1.35E-48 | -1.69E-46 |
| MS.gene051492 | MS.gene24389 | 0.802077 | 6.66E-49 | -1.69E-46 |
| MS.gene051710 | MS.gene24389 | 0.801445 | 8.97E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene24389 | 0.81183 | 5.75E-51 | -1.69E-46 |
| MS.gene052333 | MS.gene24389 | 0.821709 | 3.50E-53 | -1.69E-46 |
| MS.gene052867 | MS.gene24389 | 0.820819 | 5.61E-53 | -1.69E-46 |
| MS.gene053166 | MS.gene24389 | 0.818226 | 2.19E-52 | -1.69E-46 |
| MS.gene053641 | MS.gene24389 | 0.817176 | 3.78E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene24389 | 0.807571 | 4.74E-50 | -1.69E-46 |
| MS.gene054291 | MS.gene24389 | -0.836897 | 7.15E-57 | -1.69E-46 |
| MS.gene054467 | MS.gene24389 | 0.818895 | 1.54E-52 | -1.69E-46 |
| MS.gene054468 | MS.gene24389 | 0.813227 | 2.85E-51 | -1.69E-46 |
| MS.gene054581 | MS.gene24389 | 0.803408 | 3.54E-49 | -1.69E-46 |
| MS.gene05468 | MS.gene24389 | 0.81493 | 1.20E-51 | -1.69E-46 |
| MS.gene054775 | MS.gene24389 | 0.8302 | 3.36E-55 | -1.69E-46 |
| MS.gene054801 | MS.gene24389 | 0.815423 | 9.32E-52 | -1.69E-46 |
| MS.gene055356 | MS.gene24389 | 0.801166 | 1.02E-48 | -1.69E-46 |
| MS.gene055357 | MS.gene24389 | 0.802064 | 6.70E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene24389 | 0.823037 | 1.72E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene24389 | 0.81671 | 4.81E-52 | -1.69E-46 |
| MS.gene056050 | MS.gene24389 | 0.805932 | 1.05E-49 | -1.69E-46 |
| MS.gene056173 | MS.gene24389 | 0.806644 | 7.44E-50 | -1.69E-46 |
| MS.gene056366 | MS.gene24389 | 0.805673 | 1.19E-49 | -1.69E-46 |
| MS.gene058515 | MS.gene24389 | 0.81441 | 1.56E-51 | -1.69E-46 |
| MS.gene059609 | MS.gene24389 | 0.801802 | 7.58E-49 | -1.69E-46 |
| MS.gene060137 | MS.gene24389 | -0.811847 | 5.70E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24389.t1 | MTR_1g019670 | 95.699 | 93 | 4 | 0 | 1 | 93 | 1 | 93 | 3.92e-63 | 186 |
| MS.gene24389.t1 | MTR_1g019640 | 68.085 | 94 | 29 | 1 | 1 | 93 | 1 | 94 | 4.23e-40 | 128 |
| MS.gene24389.t1 | MTR_1g019610 | 67.021 | 94 | 30 | 1 | 1 | 93 | 1 | 94 | 1.97e-39 | 126 |
| MS.gene24389.t1 | MTR_1g019660 | 65.957 | 94 | 31 | 1 | 1 | 93 | 1 | 94 | 1.31e-38 | 124 |
| MS.gene24389.t1 | MTR_1g019600 | 45.556 | 90 | 47 | 2 | 1 | 88 | 5 | 94 | 3.66e-20 | 77.8 |
| MS.gene24389.t1 | MTR_1g019590 | 42.391 | 92 | 48 | 2 | 1 | 87 | 5 | 96 | 2.04e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAATTGTCTTCTCTCTATC+AGG | 0.235282 | 1.1:+3460744 | None:intergenic |
| TATCTTCTTGTCAAAGGGTT+TGG | 0.309945 | 1.1:-3460507 | MS.gene24389:CDS |
| GATTTACGTTCCTATGTTCC+TGG | 0.332407 | 1.1:-3460615 | MS.gene24389:CDS |
| CAAAGCAGAAAGGAAAATAA+TGG | 0.385640 | 1.1:-3460709 | MS.gene24389:CDS |
| ACTAATTTGTCCATCATTGT+TGG | 0.414841 | 1.1:+3460557 | None:intergenic |
| AAGGAAAATAATGGAAGTAT+TGG | 0.434553 | 1.1:-3460700 | MS.gene24389:CDS |
| TCTCTATCAGGGCGTAGTAT+AGG | 0.452488 | 1.1:+3460756 | None:intergenic |
| TTGATTATCTTCTTGTCAAA+GGG | 0.453582 | 1.1:-3460512 | MS.gene24389:CDS |
| GTTGATTATCTTCTTGTCAA+AGG | 0.460253 | 1.1:-3460513 | MS.gene24389:CDS |
| AATATTGATGCCAACAATGA+TGG | 0.465249 | 1.1:-3460567 | MS.gene24389:CDS |
| CAACGCATGGCTCTCCATCC+AGG | 0.488249 | 1.1:+3460597 | None:intergenic |
| TACGTTCCTATGTTCCTGGA+TGG | 0.524072 | 1.1:-3460611 | MS.gene24389:CDS |
| ATGGAGAGCCATGCGTTGTA+TGG | 0.529428 | 1.1:-3460592 | MS.gene24389:CDS |
| AACAATGATGGACAAATTAG+TGG | 0.554056 | 1.1:-3460555 | MS.gene24389:CDS |
| AAGATCAAATCAAAGCAGAA+AGG | 0.570009 | 1.1:-3460719 | MS.gene24389:CDS |
| GGCTCTCCATCCAGGAACAT+AGG | 0.575847 | 1.1:+3460605 | None:intergenic |
| AATATTGACCATACAACGCA+TGG | 0.602870 | 1.1:+3460584 | None:intergenic |
| AAATTGTCTTCTCTCTATCA+GGG | 0.603477 | 1.1:+3460745 | None:intergenic |
| TGATGGACAAATTAGTGGTG+AGG | 0.626914 | 1.1:-3460550 | MS.gene24389:CDS |
| AAAGCAGATAGCAATAAGGA+CGG | 0.681326 | 1.1:-3460675 | MS.gene24389:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGATTATCTTCTTGTCAAA+GGG | - | chr1.1:3460747-3460766 | MS.gene24389:CDS | 25.0% |
| !! | AAGGAAAATAATGGAAGTAT+TGG | - | chr1.1:3460559-3460578 | MS.gene24389:CDS | 25.0% |
| AAATTGTCTTCTCTCTATCA+GGG | + | chr1.1:3460517-3460536 | None:intergenic | 30.0% | |
| AAGATCAAATCAAAGCAGAA+AGG | - | chr1.1:3460540-3460559 | MS.gene24389:CDS | 30.0% | |
| AATATTGATGCCAACAATGA+TGG | - | chr1.1:3460692-3460711 | MS.gene24389:CDS | 30.0% | |
| CAAAGCAGAAAGGAAAATAA+TGG | - | chr1.1:3460550-3460569 | MS.gene24389:CDS | 30.0% | |
| GTTGATTATCTTCTTGTCAA+AGG | - | chr1.1:3460746-3460765 | MS.gene24389:CDS | 30.0% | |
| ! | ACTAATTTGTCCATCATTGT+TGG | + | chr1.1:3460705-3460724 | None:intergenic | 30.0% |
| !! | AACAATGATGGACAAATTAG+TGG | - | chr1.1:3460704-3460723 | MS.gene24389:CDS | 30.0% |
| AAAGCAGATAGCAATAAGGA+CGG | - | chr1.1:3460584-3460603 | MS.gene24389:CDS | 35.0% | |
| AATATTGACCATACAACGCA+TGG | + | chr1.1:3460678-3460697 | None:intergenic | 35.0% | |
| GAAATTGTCTTCTCTCTATC+AGG | + | chr1.1:3460518-3460537 | None:intergenic | 35.0% | |
| GGAAAAAGCAGATAGCAATA+AGG | - | chr1.1:3460580-3460599 | MS.gene24389:CDS | 35.0% | |
| TATCTTCTTGTCAAAGGGTT+TGG | - | chr1.1:3460752-3460771 | MS.gene24389:CDS | 35.0% | |
| GATTTACGTTCCTATGTTCC+TGG | - | chr1.1:3460644-3460663 | MS.gene24389:CDS | 40.0% | |
| ! | TGATGGACAAATTAGTGGTG+AGG | - | chr1.1:3460709-3460728 | MS.gene24389:CDS | 40.0% |
| TACGTTCCTATGTTCCTGGA+TGG | - | chr1.1:3460648-3460667 | MS.gene24389:CDS | 45.0% | |
| TCTCTATCAGGGCGTAGTAT+AGG | + | chr1.1:3460506-3460525 | None:intergenic | 45.0% | |
| ATGGAGAGCCATGCGTTGTA+TGG | - | chr1.1:3460667-3460686 | MS.gene24389:CDS | 50.0% | |
| GGCTCTCCATCCAGGAACAT+AGG | + | chr1.1:3460657-3460676 | None:intergenic | 55.0% | |
| CAACGCATGGCTCTCCATCC+AGG | + | chr1.1:3460665-3460684 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 3460500 | 3460781 | 3460500 | ID=MS.gene24389 |
| chr1.1 | mRNA | 3460500 | 3460781 | 3460500 | ID=MS.gene24389.t1;Parent=MS.gene24389 |
| chr1.1 | exon | 3460500 | 3460781 | 3460500 | ID=MS.gene24389.t1.exon1;Parent=MS.gene24389.t1 |
| chr1.1 | CDS | 3460500 | 3460781 | 3460500 | ID=cds.MS.gene24389.t1;Parent=MS.gene24389.t1 |
| Gene Sequence |
| Protein sequence |
