Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24720.t1 | XP_003625784.1 | 95.6 | 248 | 11 | 0 | 1 | 248 | 108 | 355 | 1.60E-136 | 495.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24720.t1 | Q9LZS7 | 52.7 | 241 | 106 | 4 | 3 | 238 | 112 | 349 | 6.0e-68 | 258.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24720.t1 | G7L605 | 95.6 | 248 | 11 | 0 | 1 | 248 | 108 | 355 | 1.1e-136 | 495.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050082 | MS.gene24720 | 0.800855 | 1.18E-48 | -1.69E-46 |
| MS.gene050323 | MS.gene24720 | -0.803491 | 3.40E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene24720 | 0.937497 | 3.15E-98 | -1.69E-46 |
| MS.gene050444 | MS.gene24720 | -0.810292 | 1.24E-50 | -1.69E-46 |
| MS.gene050707 | MS.gene24720 | 0.820147 | 8.00E-53 | -1.69E-46 |
| MS.gene050904 | MS.gene24720 | -0.800492 | 1.41E-48 | -1.69E-46 |
| MS.gene052129 | MS.gene24720 | 0.824958 | 6.09E-54 | -1.69E-46 |
| MS.gene052143 | MS.gene24720 | 0.8141 | 1.83E-51 | -1.69E-46 |
| MS.gene052383 | MS.gene24720 | 0.800051 | 1.73E-48 | -1.69E-46 |
| MS.gene054941 | MS.gene24720 | 0.817363 | 3.43E-52 | -1.69E-46 |
| MS.gene055251 | MS.gene24720 | 0.828394 | 9.21E-55 | -1.69E-46 |
| MS.gene055748 | MS.gene24720 | -0.827369 | 1.62E-54 | -1.69E-46 |
| MS.gene055751 | MS.gene24720 | -0.830383 | 3.03E-55 | -1.69E-46 |
| MS.gene05589 | MS.gene24720 | -0.821082 | 4.88E-53 | -1.69E-46 |
| MS.gene056387 | MS.gene24720 | -0.810654 | 1.04E-50 | -1.69E-46 |
| MS.gene056618 | MS.gene24720 | 0.809676 | 1.68E-50 | -1.69E-46 |
| MS.gene056694 | MS.gene24720 | 0.818971 | 1.48E-52 | -1.69E-46 |
| MS.gene056697 | MS.gene24720 | 0.81375 | 2.19E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene24720 | 0.819148 | 1.35E-52 | -1.69E-46 |
| MS.gene057717 | MS.gene24720 | 0.813786 | 2.15E-51 | -1.69E-46 |
| MS.gene058382 | MS.gene24720 | 0.806939 | 6.45E-50 | -1.69E-46 |
| MS.gene058838 | MS.gene24720 | -0.805822 | 1.11E-49 | -1.69E-46 |
| MS.gene058853 | MS.gene24720 | 0.820102 | 8.19E-53 | -1.69E-46 |
| MS.gene05926 | MS.gene24720 | 0.821085 | 4.87E-53 | -1.69E-46 |
| MS.gene060823 | MS.gene24720 | 0.817352 | 3.45E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene24720 | -0.805484 | 1.31E-49 | -1.69E-46 |
| MS.gene061044 | MS.gene24720 | 0.827205 | 1.78E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24720.t1 | MTR_7g104220 | 95.565 | 248 | 11 | 0 | 1 | 248 | 108 | 355 | 1.59e-180 | 499 |
| MS.gene24720.t1 | MTR_1g060490 | 62.288 | 236 | 87 | 2 | 3 | 238 | 106 | 339 | 1.84e-111 | 324 |
| MS.gene24720.t1 | MTR_1g060500 | 57.087 | 254 | 100 | 5 | 1 | 247 | 108 | 359 | 3.24e-100 | 296 |
| MS.gene24720.t1 | MTR_1g060520 | 50.403 | 248 | 87 | 6 | 1 | 246 | 108 | 321 | 1.26e-80 | 245 |
| MS.gene24720.t1 | MTR_1g092740 | 47.410 | 251 | 121 | 5 | 4 | 248 | 103 | 348 | 1.55e-79 | 243 |
| MS.gene24720.t1 | MTR_1g092690 | 43.548 | 248 | 130 | 4 | 4 | 246 | 116 | 358 | 5.30e-71 | 222 |
| MS.gene24720.t1 | MTR_1g092760 | 46.444 | 239 | 121 | 3 | 5 | 238 | 105 | 341 | 6.27e-67 | 211 |
| MS.gene24720.t1 | MTR_1g092750 | 45.455 | 242 | 120 | 5 | 5 | 238 | 106 | 343 | 7.76e-65 | 206 |
| MS.gene24720.t1 | MTR_1g092710 | 40.415 | 193 | 81 | 7 | 45 | 234 | 3 | 164 | 2.27e-37 | 129 |
| MS.gene24720.t1 | MTR_1g060530 | 54.321 | 81 | 36 | 1 | 73 | 153 | 31 | 110 | 1.62e-25 | 97.8 |
| MS.gene24720.t1 | MTR_2g044100 | 28.085 | 235 | 150 | 7 | 8 | 227 | 118 | 348 | 6.60e-21 | 90.9 |
| MS.gene24720.t1 | MTR_8g022810 | 28.049 | 246 | 155 | 10 | 2 | 230 | 108 | 348 | 2.23e-19 | 86.3 |
| MS.gene24720.t1 | MTR_5g083040 | 27.074 | 229 | 136 | 12 | 27 | 235 | 131 | 348 | 7.97e-18 | 82.0 |
| MS.gene24720.t1 | MTR_8g031400 | 25.820 | 244 | 158 | 10 | 1 | 229 | 103 | 338 | 5.14e-17 | 79.7 |
| MS.gene24720.t1 | MTR_0009s0250 | 28.272 | 191 | 120 | 6 | 8 | 188 | 110 | 293 | 6.22e-17 | 79.3 |
| MS.gene24720.t1 | MTR_8g031390 | 25.847 | 236 | 152 | 10 | 8 | 229 | 111 | 337 | 5.08e-16 | 77.0 |
| MS.gene24720.t1 | MTR_8g031430 | 27.234 | 235 | 150 | 10 | 8 | 229 | 112 | 338 | 6.75e-16 | 76.3 |
| MS.gene24720.t1 | MTR_5g084770 | 25.610 | 246 | 139 | 11 | 8 | 227 | 105 | 332 | 1.57e-14 | 72.4 |
| MS.gene24720.t1 | MTR_2g033930 | 27.572 | 243 | 152 | 11 | 2 | 228 | 101 | 335 | 2.67e-14 | 71.6 |
| MS.gene24720.t1 | MTR_7g116870 | 24.545 | 220 | 141 | 8 | 30 | 228 | 135 | 350 | 7.68e-14 | 70.5 |
| MS.gene24720.t1 | MTR_3g052480 | 23.108 | 251 | 157 | 12 | 7 | 237 | 115 | 349 | 1.14e-13 | 70.1 |
| MS.gene24720.t1 | MTR_5g009100 | 26.693 | 251 | 140 | 12 | 3 | 227 | 117 | 349 | 1.53e-13 | 69.7 |
| MS.gene24720.t1 | MTR_3g052580 | 22.541 | 244 | 164 | 10 | 8 | 237 | 109 | 341 | 1.82e-13 | 69.7 |
| MS.gene24720.t1 | MTR_3g052580 | 22.541 | 244 | 164 | 10 | 8 | 237 | 110 | 342 | 2.25e-13 | 69.3 |
| MS.gene24720.t1 | MTR_8g075230 | 23.200 | 250 | 151 | 12 | 8 | 233 | 113 | 345 | 2.93e-13 | 68.9 |
| MS.gene24720.t1 | MTR_2g461580 | 26.446 | 242 | 132 | 10 | 8 | 228 | 107 | 323 | 4.63e-13 | 68.2 |
| MS.gene24720.t1 | MTR_4g122200 | 24.111 | 253 | 168 | 8 | 2 | 237 | 104 | 349 | 9.71e-13 | 67.4 |
| MS.gene24720.t1 | MTR_8g079050 | 24.303 | 251 | 152 | 11 | 3 | 228 | 111 | 348 | 1.11e-12 | 67.0 |
| MS.gene24720.t1 | MTR_5g078210 | 23.849 | 239 | 153 | 8 | 8 | 229 | 102 | 328 | 2.02e-12 | 66.2 |
| MS.gene24720.t1 | MTR_4g087985 | 23.970 | 267 | 166 | 11 | 3 | 243 | 108 | 363 | 2.63e-12 | 65.9 |
| MS.gene24720.t1 | MTR_2g033920 | 26.446 | 242 | 149 | 9 | 8 | 229 | 109 | 341 | 4.32e-12 | 65.5 |
| MS.gene24720.t1 | MTR_2g015080 | 24.710 | 259 | 154 | 13 | 3 | 234 | 113 | 357 | 4.48e-12 | 65.5 |
| MS.gene24720.t1 | MTR_1g069175 | 25.385 | 260 | 153 | 13 | 3 | 237 | 105 | 348 | 4.98e-12 | 65.1 |
| MS.gene24720.t1 | MTR_1g079530 | 26.667 | 240 | 138 | 11 | 8 | 227 | 105 | 326 | 6.71e-12 | 64.7 |
| MS.gene24720.t1 | MTR_1g025780 | 24.706 | 255 | 161 | 13 | 8 | 243 | 118 | 360 | 7.38e-12 | 64.7 |
| MS.gene24720.t1 | MTR_3g435540 | 23.293 | 249 | 165 | 8 | 5 | 237 | 98 | 336 | 7.52e-12 | 64.7 |
| MS.gene24720.t1 | MTR_5g022630 | 23.108 | 251 | 156 | 11 | 3 | 231 | 117 | 352 | 8.83e-12 | 64.3 |
| MS.gene24720.t1 | MTR_4g035353 | 24.096 | 249 | 157 | 11 | 8 | 233 | 106 | 345 | 1.51e-11 | 63.9 |
| MS.gene24720.t1 | MTR_1g079540 | 24.016 | 254 | 167 | 11 | 3 | 239 | 102 | 346 | 1.57e-11 | 63.9 |
| MS.gene24720.t1 | MTR_7g116510 | 23.228 | 254 | 166 | 9 | 3 | 237 | 96 | 339 | 1.59e-11 | 63.5 |
| MS.gene24720.t1 | MTR_1g025760 | 24.900 | 249 | 160 | 11 | 2 | 233 | 151 | 389 | 1.72e-11 | 63.9 |
| MS.gene24720.t1 | MTR_8g014910 | 23.320 | 253 | 170 | 9 | 3 | 239 | 104 | 348 | 2.16e-11 | 63.2 |
| MS.gene24720.t1 | MTR_1g030220 | 25.131 | 191 | 130 | 4 | 58 | 237 | 180 | 368 | 2.90e-11 | 63.2 |
| MS.gene24720.t1 | MTR_3g464730 | 27.869 | 244 | 136 | 13 | 8 | 227 | 105 | 332 | 4.66e-11 | 62.4 |
| MS.gene24720.t1 | MTR_0127s0040 | 24.561 | 228 | 139 | 10 | 28 | 236 | 6 | 219 | 5.77e-11 | 61.2 |
| MS.gene24720.t1 | MTR_4g019880 | 22.780 | 259 | 162 | 11 | 3 | 237 | 103 | 347 | 5.88e-11 | 62.0 |
| MS.gene24720.t1 | MTR_2g084585 | 25.000 | 256 | 161 | 8 | 3 | 239 | 102 | 345 | 6.14e-11 | 62.0 |
| MS.gene24720.t1 | MTR_1g025910 | 24.800 | 250 | 153 | 9 | 3 | 232 | 108 | 342 | 7.07e-11 | 62.0 |
| MS.gene24720.t1 | MTR_3g082830 | 26.829 | 205 | 133 | 8 | 49 | 246 | 165 | 359 | 8.05e-11 | 61.6 |
| MS.gene24720.t1 | MTR_1g030230 | 25.888 | 197 | 121 | 6 | 58 | 237 | 172 | 360 | 8.38e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24720.t1 | AT5G03610 | 52.697 | 241 | 106 | 4 | 3 | 238 | 112 | 349 | 3.45e-87 | 263 |
| MS.gene24720.t1 | AT3G09930 | 46.473 | 241 | 120 | 5 | 3 | 237 | 108 | 345 | 2.57e-76 | 235 |
| MS.gene24720.t1 | AT3G09930 | 46.473 | 241 | 120 | 5 | 3 | 237 | 108 | 345 | 2.57e-76 | 235 |
| MS.gene24720.t1 | AT5G03600 | 38.494 | 239 | 136 | 5 | 3 | 237 | 80 | 311 | 1.35e-56 | 183 |
| MS.gene24720.t1 | AT5G03600 | 38.494 | 239 | 136 | 5 | 3 | 237 | 115 | 346 | 3.04e-56 | 184 |
| MS.gene24720.t1 | AT2G36325 | 36.134 | 238 | 141 | 6 | 5 | 237 | 119 | 350 | 1.71e-48 | 164 |
| MS.gene24720.t1 | AT5G03590 | 32.353 | 238 | 114 | 8 | 5 | 237 | 56 | 251 | 1.67e-37 | 132 |
| MS.gene24720.t1 | AT5G03590 | 30.435 | 230 | 113 | 8 | 13 | 237 | 33 | 220 | 6.85e-32 | 117 |
| MS.gene24720.t1 | AT1G53920 | 26.446 | 242 | 160 | 9 | 7 | 235 | 123 | 359 | 1.80e-17 | 81.3 |
| MS.gene24720.t1 | AT1G59406 | 25.100 | 251 | 162 | 9 | 2 | 237 | 101 | 340 | 1.35e-15 | 75.5 |
| MS.gene24720.t1 | AT1G59030 | 25.100 | 251 | 162 | 9 | 2 | 237 | 101 | 340 | 1.35e-15 | 75.5 |
| MS.gene24720.t1 | AT1G58725 | 25.100 | 251 | 162 | 9 | 2 | 237 | 101 | 340 | 1.35e-15 | 75.5 |
| MS.gene24720.t1 | AT1G58430 | 27.237 | 257 | 151 | 12 | 3 | 237 | 108 | 350 | 1.64e-15 | 75.5 |
| MS.gene24720.t1 | AT2G03980 | 25.738 | 237 | 147 | 11 | 8 | 227 | 120 | 344 | 1.72e-15 | 75.5 |
| MS.gene24720.t1 | AT2G03980 | 25.738 | 237 | 147 | 11 | 8 | 227 | 120 | 344 | 1.72e-15 | 75.5 |
| MS.gene24720.t1 | AT2G03980 | 25.738 | 237 | 147 | 11 | 8 | 227 | 120 | 344 | 1.72e-15 | 75.5 |
| MS.gene24720.t1 | AT2G03980 | 25.738 | 237 | 147 | 11 | 8 | 227 | 120 | 344 | 1.72e-15 | 75.5 |
| MS.gene24720.t1 | AT2G30310 | 26.459 | 257 | 149 | 13 | 3 | 235 | 107 | 347 | 8.83e-15 | 73.2 |
| MS.gene24720.t1 | AT5G41890 | 23.770 | 244 | 165 | 8 | 5 | 229 | 107 | 348 | 1.02e-14 | 73.2 |
| MS.gene24720.t1 | AT1G20130 | 25.188 | 266 | 164 | 11 | 2 | 243 | 275 | 529 | 1.29e-14 | 73.2 |
| MS.gene24720.t1 | AT1G20130 | 25.188 | 266 | 164 | 11 | 2 | 243 | 255 | 509 | 1.58e-14 | 72.8 |
| MS.gene24720.t1 | AT1G75900 | 24.206 | 252 | 163 | 11 | 3 | 237 | 115 | 355 | 1.10e-13 | 70.1 |
| MS.gene24720.t1 | AT2G40250 | 23.922 | 255 | 159 | 10 | 2 | 233 | 107 | 349 | 1.54e-13 | 69.7 |
| MS.gene24720.t1 | AT4G10950 | 26.104 | 249 | 136 | 11 | 8 | 228 | 147 | 375 | 1.64e-13 | 69.7 |
| MS.gene24720.t1 | AT4G10950 | 25.620 | 242 | 146 | 10 | 8 | 228 | 143 | 371 | 1.84e-13 | 69.7 |
| MS.gene24720.t1 | AT1G58520 | 24.901 | 253 | 153 | 11 | 2 | 237 | 101 | 333 | 4.10e-13 | 68.2 |
| MS.gene24720.t1 | AT1G58525 | 24.901 | 253 | 153 | 11 | 2 | 237 | 101 | 333 | 4.10e-13 | 68.2 |
| MS.gene24720.t1 | AT2G24560 | 26.562 | 256 | 154 | 13 | 3 | 237 | 107 | 349 | 6.15e-13 | 67.8 |
| MS.gene24720.t1 | AT4G01130 | 24.211 | 190 | 127 | 5 | 58 | 233 | 88 | 274 | 8.99e-13 | 67.0 |
| MS.gene24720.t1 | AT1G20120 | 24.621 | 264 | 169 | 12 | 3 | 248 | 150 | 401 | 1.11e-12 | 67.4 |
| MS.gene24720.t1 | AT3G50400 | 23.016 | 252 | 163 | 9 | 8 | 237 | 113 | 355 | 1.15e-12 | 67.0 |
| MS.gene24720.t1 | AT2G23540 | 24.031 | 258 | 144 | 11 | 3 | 228 | 122 | 359 | 1.18e-12 | 67.0 |
| MS.gene24720.t1 | AT4G01130 | 24.211 | 190 | 127 | 5 | 58 | 233 | 174 | 360 | 1.20e-12 | 67.0 |
| MS.gene24720.t1 | AT5G45960 | 23.735 | 257 | 155 | 12 | 8 | 238 | 124 | 365 | 1.28e-12 | 67.0 |
| MS.gene24720.t1 | AT3G14225 | 26.531 | 245 | 149 | 10 | 4 | 227 | 110 | 344 | 1.47e-12 | 67.0 |
| MS.gene24720.t1 | AT5G18430 | 26.778 | 239 | 146 | 10 | 8 | 227 | 105 | 333 | 4.32e-12 | 65.5 |
| MS.gene24720.t1 | AT3G14225 | 26.104 | 249 | 149 | 10 | 4 | 227 | 110 | 348 | 5.67e-12 | 65.1 |
| MS.gene24720.t1 | AT5G42170 | 24.400 | 250 | 163 | 13 | 3 | 237 | 122 | 360 | 7.49e-12 | 64.7 |
| MS.gene24720.t1 | AT1G75910 | 22.745 | 255 | 159 | 8 | 2 | 237 | 99 | 334 | 1.62e-11 | 63.5 |
| MS.gene24720.t1 | AT3G14820 | 23.600 | 250 | 166 | 10 | 2 | 237 | 103 | 341 | 2.72e-11 | 63.2 |
| MS.gene24720.t1 | AT2G04570 | 25.200 | 250 | 154 | 13 | 8 | 237 | 105 | 341 | 3.67e-11 | 62.8 |
| MS.gene24720.t1 | AT3G53100 | 22.222 | 243 | 162 | 11 | 3 | 228 | 101 | 333 | 4.93e-11 | 62.4 |
| MS.gene24720.t1 | AT1G75890 | 23.506 | 251 | 167 | 9 | 2 | 236 | 148 | 389 | 6.34e-11 | 62.0 |
| MS.gene24720.t1 | AT1G75890 | 23.506 | 251 | 167 | 9 | 2 | 236 | 119 | 360 | 7.57e-11 | 61.6 |
| MS.gene24720.t1 | AT1G28600 | 24.815 | 270 | 153 | 13 | 3 | 237 | 102 | 356 | 9.35e-11 | 61.6 |
Find 46 sgRNAs with CRISPR-Local
Find 110 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACCTAAATCTGCTTTCTTT+TGG | 0.201805 | 7.4:-7281493 | MS.gene24720:CDS |
| ATGCCACAACATTTGAAATA+TGG | 0.240247 | 7.4:-7283263 | MS.gene24720:CDS |
| GAATAATGTTAAGAAATATT+TGG | 0.268269 | 7.4:-7281525 | MS.gene24720:CDS |
| TCCCAAAAGAAAGCAGATTT+AGG | 0.287655 | 7.4:+7281491 | None:intergenic |
| AACTTTGCCTTTGGTGGTAC+TGG | 0.331428 | 7.4:-7283236 | MS.gene24720:CDS |
| TGATCTGGGTTGTCATGTTT+GGG | 0.333846 | 7.4:+7283189 | None:intergenic |
| TTGATCTGGGTTGTCATGTT+TGG | 0.334896 | 7.4:+7283188 | None:intergenic |
| TCCATCTACGCACAACATTC+AGG | 0.352797 | 7.4:-7281609 | MS.gene24720:CDS |
| TGGGTTGTCATGTTTGGGCC+TGG | 0.398569 | 7.4:+7283194 | None:intergenic |
| TTGGTTCATCCTACGGAGGC+AGG | 0.408520 | 7.4:-7281467 | MS.gene24720:CDS |
| TCCTGAATGTTGTGCGTAGA+TGG | 0.420328 | 7.4:+7281608 | None:intergenic |
| CATTATTCGCATCAAAGGAT+TGG | 0.422733 | 7.4:-7281861 | MS.gene24720:CDS |
| ATTATTCGCATCAAAGGATT+GGG | 0.434736 | 7.4:-7281860 | MS.gene24720:CDS |
| GTGAATAATCTACAACCAAT+TGG | 0.439560 | 7.4:-7281821 | MS.gene24720:CDS |
| GTTGCTCTTGTCTCTGTCGC+TGG | 0.439755 | 7.4:-7283107 | MS.gene24720:CDS |
| TATGGAATGAACTTTGCCTT+TGG | 0.442540 | 7.4:-7283245 | MS.gene24720:CDS |
| TTGGGATTTGGTTCATCCTA+CGG | 0.473098 | 7.4:-7281474 | MS.gene24720:CDS |
| ACTCGTTACACGGTCACAAA+TGG | 0.476614 | 7.4:-7283074 | MS.gene24720:CDS |
| ATCAATGAAGCTATGAATGA+TGG | 0.485195 | 7.4:+7281902 | None:intergenic |
| TAAAGATTAAGAACTAAGAA+AGG | 0.489601 | 7.4:+7281659 | None:intergenic |
| ACTAGTCGTGAATCTGTTGA+AGG | 0.490024 | 7.4:+7281405 | None:intergenic |
| GAGGATGAAGCTGTCATTGA+AGG | 0.496487 | 7.4:+7281791 | None:intergenic |
| ATTCCATATTTCAAATGTTG+TGG | 0.498820 | 7.4:+7283260 | None:intergenic |
| TTGTTGGTTCAATTTGGTCA+CGG | 0.515797 | 7.4:+7281700 | None:intergenic |
| ACAAACATTATTCGCATCAA+AGG | 0.516662 | 7.4:-7281866 | MS.gene24720:CDS |
| CTTGAGTTAAGAGTGTGTTA+TGG | 0.516810 | 7.4:+7281723 | None:intergenic |
| GGAATGAACTTTGCCTTTGG+TGG | 0.526847 | 7.4:-7283242 | MS.gene24720:CDS |
| ATTGAAGGTAGACATCCAAT+TGG | 0.532120 | 7.4:+7281806 | None:intergenic |
| GGTCACAAATGGCTCGGTTG+AGG | 0.538864 | 7.4:-7283063 | MS.gene24720:intron |
| TTCGTTGCATTGTTTGAATG+AGG | 0.541281 | 7.4:+7281772 | None:intergenic |
| AACTGAATTATTGATTTCTG+AGG | 0.546149 | 7.4:+7283127 | None:intergenic |
| GTGTTTGATACGTTGAATCC+AGG | 0.559978 | 7.4:-7283212 | MS.gene24720:CDS |
| TCTTTACGATAGTTTCATGT+CGG | 0.562308 | 7.4:-7281642 | MS.gene24720:CDS |
| AAACGACTACACTCGTTACA+CGG | 0.570553 | 7.4:-7283084 | MS.gene24720:CDS |
| GCATGCCACCCTGCCTCCGT+AGG | 0.589060 | 7.4:+7281458 | None:intergenic |
| CTACTCACTCCAACACAACA+TGG | 0.590586 | 7.4:+7281575 | None:intergenic |
| TGGTTCATCCTACGGAGGCA+GGG | 0.591879 | 7.4:-7281466 | MS.gene24720:CDS |
| AAGTTGAGGCCATGTTGTGT+TGG | 0.607650 | 7.4:-7281584 | MS.gene24720:CDS |
| CTAGTCGTGAATCTGTTGAA+GGG | 0.622231 | 7.4:+7281406 | None:intergenic |
| ACAACATTCAGGACAAGTTG+AGG | 0.623353 | 7.4:-7281598 | MS.gene24720:CDS |
| GTGTGTTATGGTAAAGTGTG+AGG | 0.626613 | 7.4:+7281735 | None:intergenic |
| TTACACGGTCACAAATGGCT+CGG | 0.640697 | 7.4:-7283069 | MS.gene24720:CDS |
| AAACACACCAGTACCACCAA+AGG | 0.644534 | 7.4:+7283229 | None:intergenic |
| GGATTTGGTTCATCCTACGG+AGG | 0.678226 | 7.4:-7281471 | MS.gene24720:CDS |
| TTCATCCTACGGAGGCAGGG+TGG | 0.704100 | 7.4:-7281463 | MS.gene24720:CDS |
| TGTTATGGTAAAGTGTGAGG+AGG | 0.725810 | 7.4:+7281738 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTTAAAAAAAAAAAAAA+AGG | + | chr7.4:7282675-7282694 | None:intergenic | 0.0% |
| !! | ATTGTAAAATTAAAATTTGA+TGG | - | chr7.4:7282265-7282284 | MS.gene24720:intron | 10.0% |
| !! | TATAGTAGTAAAATATTATT+CGG | - | chr7.4:7281880-7281899 | MS.gene24720:CDS | 10.0% |
| !!! | CAAATTTTAATTTTACAATA+TGG | + | chr7.4:7282264-7282283 | None:intergenic | 10.0% |
| !!! | TGAATTTTTTGAAATAAAAA+TGG | - | chr7.4:7281678-7281697 | MS.gene24720:CDS | 10.0% |
| !! | ACAGATTAAATTATTTATTG+TGG | + | chr7.4:7282531-7282550 | None:intergenic | 15.0% |
| !! | ATGAATAAATAATTTAGCAA+GGG | - | chr7.4:7282597-7282616 | MS.gene24720:intron | 15.0% |
| !! | GAATAATGTTAAGAAATATT+TGG | - | chr7.4:7283144-7283163 | MS.gene24720:CDS | 15.0% |
| !! | TAATCTTAAACAAATTATGT+TGG | + | chr7.4:7281780-7281799 | None:intergenic | 15.0% |
| !! | TAGTAAAATATTACTATCTT+TGG | - | chr7.4:7281913-7281932 | MS.gene24720:intron | 15.0% |
| !! | TATGAATAAATAATTTAGCA+AGG | - | chr7.4:7282596-7282615 | MS.gene24720:intron | 15.0% |
| !!! | AAACCTAGTATATATATTTT+CGG | - | chr7.4:7281986-7282005 | MS.gene24720:intron | 15.0% |
| !!! | AACAATTTGTATATTTTGTA+GGG | - | chr7.4:7282740-7282759 | MS.gene24720:intron | 15.0% |
| !!! | ATTTAAATTGATCAACTTTT+TGG | + | chr7.4:7282625-7282644 | None:intergenic | 15.0% |
| !!! | TTTAAATTGATCAACTTTTT+GGG | + | chr7.4:7282624-7282643 | None:intergenic | 15.0% |
| !! | AAAGAGCAAAGAAATATTAA+GGG | + | chr7.4:7282095-7282114 | None:intergenic | 20.0% |
| !! | ATAATTTAATAAACTACACG+TGG | - | chr7.4:7281844-7281863 | MS.gene24720:CDS | 20.0% |
| !! | ATAATTTAATCTGTAACACT+AGG | - | chr7.4:7282537-7282556 | MS.gene24720:intron | 20.0% |
| !! | ATCATCTTATCTAATATTAG+AGG | + | chr7.4:7282183-7282202 | None:intergenic | 20.0% |
| !! | ATTTCTTTGCTCTTTAATAT+AGG | - | chr7.4:7282100-7282119 | MS.gene24720:intron | 20.0% |
| !! | TAAAGAGCAAAGAAATATTA+AGG | + | chr7.4:7282096-7282115 | None:intergenic | 20.0% |
| !! | TAAAGATTAAGAACTAAGAA+AGG | + | chr7.4:7283013-7283032 | None:intergenic | 20.0% |
| !! | TTACCGAAAATATATATACT+AGG | + | chr7.4:7281992-7282011 | None:intergenic | 20.0% |
| !!! | GAACAATTTGTATATTTTGT+AGG | - | chr7.4:7282739-7282758 | MS.gene24720:intron | 20.0% |
| !!! | TCAAAAGTCTAATTTGTATA+TGG | + | chr7.4:7282652-7282671 | None:intergenic | 20.0% |
| !!! | TTAAATTGATCAACTTTTTG+GGG | + | chr7.4:7282623-7282642 | None:intergenic | 20.0% |
| !!! | TTTTAATTTTACAATATGGG+TGG | + | chr7.4:7282260-7282279 | None:intergenic | 20.0% |
| ! | ATAATGAAATGATGGCATTA+AGG | - | chr7.4:7282290-7282309 | MS.gene24720:intron | 25.0% |
| ! | ATCTTAGCATTACATCTTAT+TGG | - | chr7.4:7281701-7281720 | MS.gene24720:CDS | 25.0% |
| ! | ATTCCATATTTCAAATGTTG+TGG | + | chr7.4:7281412-7281431 | None:intergenic | 25.0% |
| ! | CTGTTGAAAAAAATTGATCT+GGG | + | chr7.4:7281497-7281516 | None:intergenic | 25.0% |
| ! | GAGATCTTCTTTCATAAAAA+AGG | - | chr7.4:7282504-7282523 | MS.gene24720:intron | 25.0% |
| ! | TCTGTTGAAAAAAATTGATC+TGG | + | chr7.4:7281498-7281517 | None:intergenic | 25.0% |
| ! | TGATGGAAATAATGAAATGA+TGG | - | chr7.4:7282282-7282301 | MS.gene24720:intron | 25.0% |
| !!! | AACTGAATTATTGATTTCTG+AGG | + | chr7.4:7281545-7281564 | None:intergenic | 25.0% |
| !!! | TAGAATGATGTTTTGTTTGT+TGG | + | chr7.4:7282988-7283007 | None:intergenic | 25.0% |
| !!! | TTGGATTCTGTCTTATTTTA+AGG | + | chr7.4:7282347-7282366 | None:intergenic | 25.0% |
| !!! | TTTTGTTTGTTGGTTCAATT+TGG | + | chr7.4:7282978-7282997 | None:intergenic | 25.0% |
| ACAAACATTATTCGCATCAA+AGG | - | chr7.4:7282803-7282822 | MS.gene24720:intron | 30.0% | |
| ATCAATGAAGCTATGAATGA+TGG | + | chr7.4:7282770-7282789 | None:intergenic | 30.0% | |
| ATCATCTTGTCTAATGTTCT+TGG | + | chr7.4:7282234-7282253 | None:intergenic | 30.0% | |
| ATTATTCGCATCAAAGGATT+GGG | - | chr7.4:7282809-7282828 | MS.gene24720:intron | 30.0% | |
| GAAAGAAGATCTCAATGTAA+GGG | + | chr7.4:7282497-7282516 | None:intergenic | 30.0% | |
| GAAAGAATACCTCAATGTAA+GGG | + | chr7.4:7282452-7282471 | None:intergenic | 30.0% | |
| GTGAATAATCTACAACCAAT+TGG | - | chr7.4:7282848-7282867 | MS.gene24720:intron | 30.0% | |
| TCATCTTGTCTAATGTTCTT+GGG | + | chr7.4:7282233-7282252 | None:intergenic | 30.0% | |
| TCTTTACGATAGTTTCATGT+CGG | - | chr7.4:7283027-7283046 | MS.gene24720:intron | 30.0% | |
| TGAAAGAAGATCTCAATGTA+AGG | + | chr7.4:7282498-7282517 | None:intergenic | 30.0% | |
| TGAAAGAATACCTCAATGTA+AGG | + | chr7.4:7282453-7282472 | None:intergenic | 30.0% | |
| ! | AACCTAAATCTGCTTTCTTT+TGG | - | chr7.4:7283176-7283195 | MS.gene24720:CDS | 30.0% |
| ! | ACCTAAATCTGCTTTCTTTT+GGG | - | chr7.4:7283177-7283196 | MS.gene24720:CDS | 30.0% |
| ! | ATGCCACAACATTTGAAATA+TGG | - | chr7.4:7281406-7281425 | MS.gene24720:CDS | 30.0% |
| ! | GATACTTCTTTTCTGCTTAT+AGG | - | chr7.4:7282404-7282423 | MS.gene24720:intron | 30.0% |
| ! | TCCAAACTTTACTCATTTTC+CGG | - | chr7.4:7282362-7282381 | MS.gene24720:intron | 30.0% |
| ! | TGAGGTATTCTTTCATTGAT+AGG | - | chr7.4:7282458-7282477 | MS.gene24720:intron | 30.0% |
| !! | GAAAAGAAGTATCTACCTAA+CGG | + | chr7.4:7282397-7282416 | None:intergenic | 30.0% |
| ACCGGAAAATGAGTAAAGTT+TGG | + | chr7.4:7282366-7282385 | None:intergenic | 35.0% | |
| AGGAGGAAATGGACAAATAA+AGG | - | chr7.4:7282424-7282443 | MS.gene24720:intron | 35.0% | |
| ATAAACTACACGTGGAACTT+CGG | - | chr7.4:7281852-7281871 | MS.gene24720:CDS | 35.0% | |
| ATAAAGGAACCCTTACATTG+AGG | - | chr7.4:7282440-7282459 | MS.gene24720:intron | 35.0% | |
| ATGCTTATTAAGTGCAAACG+TGG | - | chr7.4:7281731-7281750 | MS.gene24720:CDS | 35.0% | |
| ATTGAAGGTAGACATCCAAT+TGG | + | chr7.4:7282866-7282885 | None:intergenic | 35.0% | |
| CATTATTCGCATCAAAGGAT+TGG | - | chr7.4:7282808-7282827 | MS.gene24720:intron | 35.0% | |
| CGAGTGTCATAAAGATACAA+TGG | + | chr7.4:7282072-7282091 | None:intergenic | 35.0% | |
| TATGGAATGAACTTTGCCTT+TGG | - | chr7.4:7281424-7281443 | MS.gene24720:CDS | 35.0% | |
| TCCCAAAAGAAAGCAGATTT+AGG | + | chr7.4:7283181-7283200 | None:intergenic | 35.0% | |
| TTCGTTGCATTGTTTGAATG+AGG | + | chr7.4:7282900-7282919 | None:intergenic | 35.0% | |
| TTTCTGCTTATAGGAGGAAA+TGG | - | chr7.4:7282413-7282432 | MS.gene24720:intron | 35.0% | |
| ! | ACTTCTTTTCTGCTTATAGG+AGG | - | chr7.4:7282407-7282426 | MS.gene24720:intron | 35.0% |
| ! | CTTGAGTTAAGAGTGTGTTA+TGG | + | chr7.4:7282949-7282968 | None:intergenic | 35.0% |
| !! | GTGAGTAGCAAGTATTTTTG+TGG | - | chr7.4:7283109-7283128 | MS.gene24720:CDS | 35.0% |
| !! | TTGTTGGTTCAATTTGGTCA+CGG | + | chr7.4:7282972-7282991 | None:intergenic | 35.0% |
| !!! | ATCTGCTTTCTTTTGGGATT+TGG | - | chr7.4:7283183-7283202 | MS.gene24720:CDS | 35.0% |
| AAACGACTACACTCGTTACA+CGG | - | chr7.4:7281585-7281604 | MS.gene24720:CDS | 40.0% | |
| ACAACATTCAGGACAAGTTG+AGG | - | chr7.4:7283071-7283090 | MS.gene24720:CDS | 40.0% | |
| CTAGTCGTGAATCTGTTGAA+GGG | + | chr7.4:7283266-7283285 | None:intergenic | 40.0% | |
| CTCAATGTAAGGGTTCCATT+GGG | + | chr7.4:7282487-7282506 | None:intergenic | 40.0% | |
| GTGTGTTATGGTAAAGTGTG+AGG | + | chr7.4:7282937-7282956 | None:intergenic | 40.0% | |
| GTGTTTGATACGTTGAATCC+AGG | - | chr7.4:7281457-7281476 | MS.gene24720:CDS | 40.0% | |
| TACCTAACGGTAACATTGAC+CGG | + | chr7.4:7282384-7282403 | None:intergenic | 40.0% | |
| TCTCAATGTAAGGGTTCCAT+TGG | + | chr7.4:7282488-7282507 | None:intergenic | 40.0% | |
| TGATCTGGGTTGTCATGTTT+GGG | + | chr7.4:7281483-7281502 | None:intergenic | 40.0% | |
| TGTTATGGTAAAGTGTGAGG+AGG | + | chr7.4:7282934-7282953 | None:intergenic | 40.0% | |
| TTCATTGATAGGATGCCCAA+TGG | - | chr7.4:7282469-7282488 | MS.gene24720:intron | 40.0% | |
| TTGATCTGGGTTGTCATGTT+TGG | + | chr7.4:7281484-7281503 | None:intergenic | 40.0% | |
| ! | GTGCTTTAAGCATGCTATCT+CGG | - | chr7.4:7281819-7281838 | MS.gene24720:CDS | 40.0% |
| ! | TTGGGATTTGGTTCATCCTA+CGG | - | chr7.4:7283195-7283214 | MS.gene24720:CDS | 40.0% |
| AAACACACCAGTACCACCAA+AGG | + | chr7.4:7281443-7281462 | None:intergenic | 45.0% | |
| AAGTTGAGGCCATGTTGTGT+TGG | - | chr7.4:7283085-7283104 | MS.gene24720:CDS | 45.0% | |
| ACTCGTTACACGGTCACAAA+TGG | - | chr7.4:7281595-7281614 | MS.gene24720:CDS | 45.0% | |
| CTACTCACTCCAACACAACA+TGG | + | chr7.4:7283097-7283116 | None:intergenic | 45.0% | |
| GAACCCGTAGACAATTTGAG+TGG | + | chr7.4:7282565-7282584 | None:intergenic | 45.0% | |
| GAGGATGAAGCTGTCATTGA+AGG | + | chr7.4:7282881-7282900 | None:intergenic | 45.0% | |
| GCTCCACTCAAATTGTCTAC+GGG | - | chr7.4:7282559-7282578 | MS.gene24720:intron | 45.0% | |
| GGAATGAACTTTGCCTTTGG+TGG | - | chr7.4:7281427-7281446 | MS.gene24720:CDS | 45.0% | |
| GGCTCCACTCAAATTGTCTA+CGG | - | chr7.4:7282558-7282577 | MS.gene24720:intron | 45.0% | |
| TCCATCTACGCACAACATTC+AGG | - | chr7.4:7283060-7283079 | MS.gene24720:intron | 45.0% | |
| TCCTGAATGTTGTGCGTAGA+TGG | + | chr7.4:7283064-7283083 | None:intergenic | 45.0% | |
| TTACACGGTCACAAATGGCT+CGG | - | chr7.4:7281600-7281619 | MS.gene24720:CDS | 45.0% | |
| TTCCGGTCAATGTTACCGTT+AGG | - | chr7.4:7282379-7282398 | MS.gene24720:intron | 45.0% | |
| ! | AACTTTGCCTTTGGTGGTAC+TGG | - | chr7.4:7281433-7281452 | MS.gene24720:CDS | 45.0% |
| !!! | AAATTTTAATTTTACAATAT+GGG | + | chr7.4:7282263-7282282 | None:intergenic | 5.0% |
| ! | GGATTTGGTTCATCCTACGG+AGG | - | chr7.4:7283198-7283217 | MS.gene24720:CDS | 50.0% |
| GTTGCTCTTGTCTCTGTCGC+TGG | - | chr7.4:7281562-7281581 | MS.gene24720:CDS | 55.0% | |
| TGGGTTGTCATGTTTGGGCC+TGG | + | chr7.4:7281478-7281497 | None:intergenic | 55.0% | |
| TGGTTCATCCTACGGAGGCA+GGG | - | chr7.4:7283203-7283222 | MS.gene24720:CDS | 55.0% | |
| TTGGTTCATCCTACGGAGGC+AGG | - | chr7.4:7283202-7283221 | MS.gene24720:CDS | 55.0% | |
| !! | GGTCACAAATGGCTCGGTTG+AGG | - | chr7.4:7281606-7281625 | MS.gene24720:CDS | 55.0% |
| TTCATCCTACGGAGGCAGGG+TGG | - | chr7.4:7283206-7283225 | MS.gene24720:CDS | 60.0% | |
| GCATGCCACCCTGCCTCCGT+AGG | + | chr7.4:7283214-7283233 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 7281406 | 7283285 | 7281406 | ID=MS.gene24720 |
| chr7.4 | mRNA | 7281406 | 7283285 | 7281406 | ID=MS.gene24720.t1;Parent=MS.gene24720 |
| chr7.4 | exon | 7283064 | 7283285 | 7283064 | ID=MS.gene24720.t1.exon1;Parent=MS.gene24720.t1 |
| chr7.4 | CDS | 7283064 | 7283285 | 7283064 | ID=cds.MS.gene24720.t1;Parent=MS.gene24720.t1 |
| chr7.4 | exon | 7281406 | 7281930 | 7281406 | ID=MS.gene24720.t1.exon2;Parent=MS.gene24720.t1 |
| chr7.4 | CDS | 7281406 | 7281930 | 7281406 | ID=cds.MS.gene24720.t1;Parent=MS.gene24720.t1 |
| Gene Sequence |
| Protein sequence |
