Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24760.t1 | XP_013450209.1 | 93.1 | 102 | 7 | 0 | 1 | 102 | 1 | 102 | 3.50E-41 | 177.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24760.t1 | Q8RWM3 | 54.8 | 84 | 37 | 1 | 21 | 104 | 21 | 103 | 4.6e-18 | 92.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24760.t1 | A0A072U3D2 | 93.1 | 102 | 7 | 0 | 1 | 102 | 1 | 102 | 2.5e-41 | 177.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene24760 | -0.808451 | 3.08E-50 | -1.69E-46 |
| MS.gene049378 | MS.gene24760 | -0.849298 | 3.54E-60 | -1.69E-46 |
| MS.gene049669 | MS.gene24760 | -0.814159 | 1.78E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene24760 | -0.801095 | 1.06E-48 | -1.69E-46 |
| MS.gene050227 | MS.gene24760 | -0.831051 | 2.08E-55 | -1.69E-46 |
| MS.gene050228 | MS.gene24760 | -0.83215 | 1.11E-55 | -1.69E-46 |
| MS.gene050330 | MS.gene24760 | 0.818354 | 2.05E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene24760 | 0.8266 | 2.48E-54 | -1.69E-46 |
| MS.gene050719 | MS.gene24760 | -0.805414 | 1.35E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene24760 | -0.806841 | 6.76E-50 | -1.69E-46 |
| MS.gene050750 | MS.gene24760 | -0.856152 | 3.90E-62 | -1.69E-46 |
| MS.gene050787 | MS.gene24760 | -0.833592 | 4.88E-56 | -1.69E-46 |
| MS.gene050820 | MS.gene24760 | -0.811562 | 6.58E-51 | -1.69E-46 |
| MS.gene050953 | MS.gene24760 | -0.838166 | 3.38E-57 | -1.69E-46 |
| MS.gene05119 | MS.gene24760 | 0.810192 | 1.30E-50 | -1.69E-46 |
| MS.gene052176 | MS.gene24760 | -0.860902 | 1.49E-63 | -1.69E-46 |
| MS.gene052309 | MS.gene24760 | 0.857579 | 1.48E-62 | -1.69E-46 |
| MS.gene052333 | MS.gene24760 | -0.813258 | 2.80E-51 | -1.69E-46 |
| MS.gene052358 | MS.gene24760 | 0.883027 | 6.35E-71 | -1.69E-46 |
| MS.gene052361 | MS.gene24760 | 0.829713 | 4.41E-55 | -1.69E-46 |
| MS.gene052376 | MS.gene24760 | -0.811855 | 5.68E-51 | -1.69E-46 |
| MS.gene052389 | MS.gene24760 | -0.826041 | 3.37E-54 | -1.69E-46 |
| MS.gene052586 | MS.gene24760 | -0.83007 | 3.61E-55 | -1.69E-46 |
| MS.gene052965 | MS.gene24760 | 0.822069 | 2.89E-53 | -1.69E-46 |
| MS.gene053054 | MS.gene24760 | 0.829039 | 6.43E-55 | -1.69E-46 |
| MS.gene05414 | MS.gene24760 | -0.803563 | 3.28E-49 | -1.69E-46 |
| MS.gene054291 | MS.gene24760 | 0.853661 | 2.06E-61 | -1.69E-46 |
| MS.gene054405 | MS.gene24760 | -0.833273 | 5.86E-56 | -1.69E-46 |
| MS.gene054468 | MS.gene24760 | -0.833171 | 6.22E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene24760 | -0.827311 | 1.68E-54 | -1.69E-46 |
| MS.gene055701 | MS.gene24760 | -0.803064 | 4.17E-49 | -1.69E-46 |
| MS.gene056087 | MS.gene24760 | -0.831663 | 1.47E-55 | -1.69E-46 |
| MS.gene056506 | MS.gene24760 | -0.823527 | 1.32E-53 | -1.69E-46 |
| MS.gene057026 | MS.gene24760 | -0.823458 | 1.37E-53 | -1.69E-46 |
| MS.gene05761 | MS.gene24760 | -0.81288 | 3.39E-51 | -1.69E-46 |
| MS.gene057967 | MS.gene24760 | -0.804152 | 2.48E-49 | -1.69E-46 |
| MS.gene059033 | MS.gene24760 | 0.815444 | 9.22E-52 | -1.69E-46 |
| MS.gene059249 | MS.gene24760 | -0.813608 | 2.35E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene24760 | -0.821465 | 3.98E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene24760 | -0.832817 | 7.61E-56 | -1.69E-46 |
| MS.gene060572 | MS.gene24760 | -0.829144 | 6.07E-55 | -1.69E-46 |
| MS.gene060634 | MS.gene24760 | 0.826657 | 2.41E-54 | -1.69E-46 |
| MS.gene060879 | MS.gene24760 | -0.837438 | 5.20E-57 | -1.69E-46 |
| MS.gene061040 | MS.gene24760 | 0.92321 | 3.59E-89 | -1.69E-46 |
| MS.gene061114 | MS.gene24760 | 0.849198 | 3.77E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24760.t1 | MTR_7g106200 | 91.250 | 80 | 7 | 0 | 23 | 102 | 23 | 102 | 1.56e-44 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene24760.t1 | AT3G09180 | 54.762 | 84 | 37 | 1 | 21 | 104 | 21 | 103 | 1.53e-25 | 98.6 |
| MS.gene24760.t1 | AT3G09180 | 54.762 | 84 | 37 | 1 | 21 | 104 | 21 | 103 | 2.45e-25 | 98.6 |
| MS.gene24760.t1 | AT3G09180 | 54.762 | 84 | 37 | 1 | 21 | 104 | 21 | 103 | 2.45e-25 | 98.6 |
Find 34 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGACTTCGCGATTAGGGTTT+TGG | 0.228422 | 7.1:+6363630 | MS.gene24760:CDS |
| TGGAGGAGGGAGGAGGAGTG+GGG | 0.303544 | 7.1:-6363357 | None:intergenic |
| GGTGGAGGAGGGAGGAGGAG+TGG | 0.378647 | 7.1:-6363359 | None:intergenic |
| AGGTGCTTCCGCGGTGGAGG+AGG | 0.394926 | 7.1:-6363371 | None:intergenic |
| GGAGGAGGGAGGAGGAGTGG+GGG | 0.401426 | 7.1:-6363356 | None:intergenic |
| GTGGAGGAGGGAGGAGGAGT+GGG | 0.415547 | 7.1:-6363358 | None:intergenic |
| CGCGGAAGCACCTCCGAAGC+AGG | 0.440793 | 7.1:+6363381 | MS.gene24760:CDS |
| ATCATCGCCGACATCCGAAT+TGG | 0.448405 | 7.1:+6363442 | MS.gene24760:CDS |
| ATAGCTAGAGCAACCTGCTT+CGG | 0.468530 | 7.1:-6363394 | None:intergenic |
| TCCGCGGTGGAGGAGGGAGG+AGG | 0.470294 | 7.1:-6363364 | None:intergenic |
| CGGAGGTGCTTCCGCGGTGG+AGG | 0.483398 | 7.1:-6363374 | None:intergenic |
| GGTGCTTCCGCGGTGGAGGA+GGG | 0.499035 | 7.1:-6363370 | None:intergenic |
| TTTAAAGACCTTCGATCCCT+CGG | 0.507856 | 7.1:+6363571 | MS.gene24760:CDS |
| GGAGACGATCAGCGCCAATT+CGG | 0.516484 | 7.1:-6363456 | None:intergenic |
| AACGCATACCGAGGGATCGA+AGG | 0.518466 | 7.1:-6363579 | None:intergenic |
| GATGTCGGCGATGATACGTT+CGG | 0.535062 | 7.1:-6363434 | None:intergenic |
| GAAGCAGGTTGCTCTAGCTA+TGG | 0.535644 | 7.1:+6363396 | MS.gene24760:CDS |
| CAGCGATGAATAGAGCTTCG+AGG | 0.547357 | 7.1:-6363477 | None:intergenic |
| CTGCTTCGGAGGTGCTTCCG+CGG | 0.550284 | 7.1:-6363380 | None:intergenic |
| GAAGCTCTATTCATCGCTGC+TGG | 0.552451 | 7.1:+6363481 | MS.gene24760:CDS |
| TCTTTGACGAAAACTTGAAG+AGG | 0.553058 | 7.1:-6363526 | None:intergenic |
| GCTTCCGCGGTGGAGGAGGG+AGG | 0.559888 | 7.1:-6363367 | None:intergenic |
| GGTTGAGAAAGTTGCCGCCG+TGG | 0.577460 | 7.1:-6363335 | None:intergenic |
| ATCAGCGCCAATTCGGATGT+CGG | 0.583731 | 7.1:-6363449 | None:intergenic |
| GCTAGAGCAACCTGCTTCGG+AGG | 0.598630 | 7.1:-6363391 | None:intergenic |
| CTTCGGAGGTGCTTCCGCGG+TGG | 0.602149 | 7.1:-6363377 | None:intergenic |
| GCTGCTGGTCAACCTCATCA+AGG | 0.602170 | 7.1:+6363496 | MS.gene24760:CDS |
| GAAACTGAAAACGCATACCG+AGG | 0.617260 | 7.1:-6363588 | None:intergenic |
| AGAGGCTTATTGCCTTGATG+AGG | 0.633931 | 7.1:-6363508 | None:intergenic |
| GCAACAAACTCAGCAAGCCA+CGG | 0.636504 | 7.1:+6363318 | MS.gene24760:CDS |
| TCCTCCTCCCTCCTCCACCG+CGG | 0.648019 | 7.1:+6363363 | MS.gene24760:CDS |
| AAACTGAAAACGCATACCGA+GGG | 0.652782 | 7.1:-6363587 | None:intergenic |
| GCTCTAGCTATGGACAAACT+CGG | 0.684631 | 7.1:+6363406 | MS.gene24760:CDS |
| ACAAACTCAGCAAGCCACGG+CGG | 0.712432 | 7.1:+6363321 | MS.gene24760:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TCTTTGACGAAAACTTGAAG+AGG | - | chr7.1:6363529-6363548 | None:intergenic | 35.0% | |
| !!! | TGATTTTGACTTCGCGATTA+GGG | + | chr7.1:6363624-6363643 | MS.gene24760:CDS | 35.0% |
| !!! | TTGATTTTGACTTCGCGATT+AGG | + | chr7.1:6363623-6363642 | MS.gene24760:CDS | 35.0% |
| AAACTGAAAACGCATACCGA+GGG | - | chr7.1:6363590-6363609 | None:intergenic | 40.0% | |
| TTTAAAGACCTTCGATCCCT+CGG | + | chr7.1:6363571-6363590 | MS.gene24760:CDS | 40.0% | |
| !! | GATTAGGGTTTTGGTATCGT+CGG | + | chr7.1:6363639-6363658 | MS.gene24760:CDS | 40.0% |
| AGAGGCTTATTGCCTTGATG+AGG | - | chr7.1:6363511-6363530 | None:intergenic | 45.0% | |
| ATAGCTAGAGCAACCTGCTT+CGG | - | chr7.1:6363397-6363416 | None:intergenic | 45.0% | |
| GAAACTGAAAACGCATACCG+AGG | - | chr7.1:6363591-6363610 | None:intergenic | 45.0% | |
| GCTCTAGCTATGGACAAACT+CGG | + | chr7.1:6363406-6363425 | MS.gene24760:CDS | 45.0% | |
| ! | TGACTTCGCGATTAGGGTTT+TGG | + | chr7.1:6363630-6363649 | MS.gene24760:CDS | 45.0% |
| ATCAGCGCCAATTCGGATGT+CGG | - | chr7.1:6363452-6363471 | None:intergenic | 50.0% | |
| ATCATCGCCGACATCCGAAT+TGG | + | chr7.1:6363442-6363461 | MS.gene24760:CDS | 50.0% | |
| CAGCGATGAATAGAGCTTCG+AGG | - | chr7.1:6363480-6363499 | None:intergenic | 50.0% | |
| GAAGCAGGTTGCTCTAGCTA+TGG | + | chr7.1:6363396-6363415 | MS.gene24760:CDS | 50.0% | |
| GAAGCTCTATTCATCGCTGC+TGG | + | chr7.1:6363481-6363500 | MS.gene24760:CDS | 50.0% | |
| GATGTCGGCGATGATACGTT+CGG | - | chr7.1:6363437-6363456 | None:intergenic | 50.0% | |
| GCAACAAACTCAGCAAGCCA+CGG | + | chr7.1:6363318-6363337 | MS.gene24760:CDS | 50.0% | |
| AACGCATACCGAGGGATCGA+AGG | - | chr7.1:6363582-6363601 | None:intergenic | 55.0% | |
| ACAAACTCAGCAAGCCACGG+CGG | + | chr7.1:6363321-6363340 | MS.gene24760:CDS | 55.0% | |
| GCTGCTGGTCAACCTCATCA+AGG | + | chr7.1:6363496-6363515 | MS.gene24760:CDS | 55.0% | |
| GGAGACGATCAGCGCCAATT+CGG | - | chr7.1:6363459-6363478 | None:intergenic | 55.0% | |
| GCTAGAGCAACCTGCTTCGG+AGG | - | chr7.1:6363394-6363413 | None:intergenic | 60.0% | |
| GGTTGAGAAAGTTGCCGCCG+TGG | - | chr7.1:6363338-6363357 | None:intergenic | 60.0% | |
| GTGGAGGAGGGAGGAGGAGT+GGG | - | chr7.1:6363361-6363380 | None:intergenic | 65.0% | |
| TGGAGGAGGGAGGAGGAGTG+GGG | - | chr7.1:6363360-6363379 | None:intergenic | 65.0% | |
| !! | CTGCTTCGGAGGTGCTTCCG+CGG | - | chr7.1:6363383-6363402 | None:intergenic | 65.0% |
| AGGTGCTTCCGCGGTGGAGG+AGG | - | chr7.1:6363374-6363393 | None:intergenic | 70.0% | |
| GGAGGAGGGAGGAGGAGTGG+GGG | - | chr7.1:6363359-6363378 | None:intergenic | 70.0% | |
| GGTGCTTCCGCGGTGGAGGA+GGG | - | chr7.1:6363373-6363392 | None:intergenic | 70.0% | |
| GGTGGAGGAGGGAGGAGGAG+TGG | - | chr7.1:6363362-6363381 | None:intergenic | 70.0% | |
| TCCTCCTCCCTCCTCCACCG+CGG | + | chr7.1:6363363-6363382 | MS.gene24760:CDS | 70.0% | |
| !! | CGCGGAAGCACCTCCGAAGC+AGG | + | chr7.1:6363381-6363400 | MS.gene24760:CDS | 70.0% |
| !! | CTTCGGAGGTGCTTCCGCGG+TGG | - | chr7.1:6363380-6363399 | None:intergenic | 70.0% |
| GCTTCCGCGGTGGAGGAGGG+AGG | - | chr7.1:6363370-6363389 | None:intergenic | 75.0% | |
| TCCGCGGTGGAGGAGGGAGG+AGG | - | chr7.1:6363367-6363386 | None:intergenic | 75.0% | |
| !! | CGGAGGTGCTTCCGCGGTGG+AGG | - | chr7.1:6363377-6363396 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 6363316 | 6363672 | 6363316 | ID=MS.gene24760 |
| chr7.1 | mRNA | 6363316 | 6363672 | 6363316 | ID=MS.gene24760.t1;Parent=MS.gene24760 |
| chr7.1 | exon | 6363316 | 6363672 | 6363316 | ID=MS.gene24760.t1.exon1;Parent=MS.gene24760.t1 |
| chr7.1 | CDS | 6363316 | 6363672 | 6363316 | ID=cds.MS.gene24760.t1;Parent=MS.gene24760.t1 |
| Gene Sequence |
| Protein sequence |
