Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25293.t1 | AET03055.2 | 96.7 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 1.30E-23 | 118.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25293.t1 | Q9XIE6 | 59.2 | 49 | 20 | 0 | 12 | 60 | 4 | 52 | 2.1e-09 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25293.t1 | G7LDU7 | 96.7 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 9.5e-24 | 118.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050084 | MS.gene25293 | 0.815566 | 8.66E-52 | -1.69E-46 |
| MS.gene050085 | MS.gene25293 | 0.828548 | 8.46E-55 | -1.69E-46 |
| MS.gene050317 | MS.gene25293 | 0.802285 | 6.03E-49 | -1.69E-46 |
| MS.gene050359 | MS.gene25293 | 0.846138 | 2.62E-59 | -1.69E-46 |
| MS.gene050361 | MS.gene25293 | 0.852651 | 4.01E-61 | -1.69E-46 |
| MS.gene050384 | MS.gene25293 | 0.812825 | 3.49E-51 | -1.69E-46 |
| MS.gene050386 | MS.gene25293 | 0.828551 | 8.44E-55 | -1.69E-46 |
| MS.gene050556 | MS.gene25293 | 0.802383 | 5.76E-49 | -1.69E-46 |
| MS.gene050646 | MS.gene25293 | 0.837003 | 6.72E-57 | -1.69E-46 |
| MS.gene050654 | MS.gene25293 | 0.840757 | 7.17E-58 | -1.69E-46 |
| MS.gene050941 | MS.gene25293 | 0.80294 | 4.42E-49 | -1.69E-46 |
| MS.gene051233 | MS.gene25293 | 0.800013 | 1.76E-48 | -1.69E-46 |
| MS.gene051343 | MS.gene25293 | 0.835475 | 1.64E-56 | -1.69E-46 |
| MS.gene051381 | MS.gene25293 | 0.804835 | 1.79E-49 | -1.69E-46 |
| MS.gene052242 | MS.gene25293 | -0.880156 | 6.92E-70 | -1.69E-46 |
| MS.gene052307 | MS.gene25293 | 0.80211 | 6.55E-49 | -1.69E-46 |
| MS.gene052609 | MS.gene25293 | 0.837994 | 3.74E-57 | -1.69E-46 |
| MS.gene052776 | MS.gene25293 | 0.810669 | 1.03E-50 | -1.69E-46 |
| MS.gene052932 | MS.gene25293 | 0.81742 | 3.33E-52 | -1.69E-46 |
| MS.gene052994 | MS.gene25293 | 0.825688 | 4.09E-54 | -1.69E-46 |
| MS.gene053058 | MS.gene25293 | 0.810767 | 9.78E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene25293 | 0.823831 | 1.12E-53 | -1.69E-46 |
| MS.gene055311 | MS.gene25293 | 0.84116 | 5.62E-58 | -1.69E-46 |
| MS.gene055313 | MS.gene25293 | 0.814877 | 1.23E-51 | -1.69E-46 |
| MS.gene055314 | MS.gene25293 | 0.807255 | 5.53E-50 | -1.69E-46 |
| MS.gene056045 | MS.gene25293 | 0.817066 | 4.00E-52 | -1.69E-46 |
| MS.gene056097 | MS.gene25293 | 0.827345 | 1.65E-54 | -1.69E-46 |
| MS.gene056181 | MS.gene25293 | 0.829839 | 4.11E-55 | -1.69E-46 |
| MS.gene056480 | MS.gene25293 | 0.802828 | 4.66E-49 | -1.69E-46 |
| MS.gene056787 | MS.gene25293 | 0.846664 | 1.88E-59 | -1.69E-46 |
| MS.gene056849 | MS.gene25293 | 0.803171 | 3.96E-49 | -1.69E-46 |
| MS.gene057890 | MS.gene25293 | -0.802289 | 6.02E-49 | -1.69E-46 |
| MS.gene058054 | MS.gene25293 | 0.809009 | 2.34E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene25293 | 0.802177 | 6.35E-49 | -1.69E-46 |
| MS.gene058638 | MS.gene25293 | 0.822388 | 2.43E-53 | -1.69E-46 |
| MS.gene058639 | MS.gene25293 | 0.824859 | 6.42E-54 | -1.69E-46 |
| MS.gene058758 | MS.gene25293 | 0.813501 | 2.48E-51 | -1.69E-46 |
| MS.gene059007 | MS.gene25293 | 0.846285 | 2.39E-59 | -1.69E-46 |
| MS.gene059965 | MS.gene25293 | 0.80519 | 1.50E-49 | -1.69E-46 |
| MS.gene06010 | MS.gene25293 | 0.810529 | 1.10E-50 | -1.69E-46 |
| MS.gene060259 | MS.gene25293 | 0.806802 | 6.89E-50 | -1.69E-46 |
| MS.gene060869 | MS.gene25293 | 0.804485 | 2.11E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene25293 | 0.808491 | 3.02E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene25293 | 0.829955 | 3.85E-55 | -1.69E-46 |
| MS.gene061006 | MS.gene25293 | 0.80972 | 1.65E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene25293 | 0.803807 | 2.92E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25293.t1 | MTR_8g062150 | 96.667 | 60 | 2 | 0 | 1 | 60 | 1 | 60 | 1.23e-35 | 126 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25293.t1 | AT1G59820 | 59.184 | 49 | 20 | 0 | 12 | 60 | 4 | 52 | 5.89e-14 | 65.5 |
Find 17 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAATTGAAGAAATGATGA+AGG | 0.448024 | 8.2:+38372148 | None:intergenic |
| CCATCGAGTTCCTTCTCAAT+CGG | 0.452726 | 8.2:+38372229 | MS.gene25293:CDS |
| AAACGAACCTTGAATCGAAC+AGG | 0.479166 | 8.2:-38372326 | None:intergenic |
| CAGAAAATAGTGCGATAAGA+AGG | 0.492848 | 8.2:-38372281 | None:intergenic |
| TTGAAGAAATGATGAAGGGA+TGG | 0.516573 | 8.2:+38372153 | None:intergenic |
| CCTTCTCAATCGGTTCGACT+CGG | 0.521085 | 8.2:+38372239 | MS.gene25293:CDS |
| AAATGATGAAGGGATGGGAT+GGG | 0.523807 | 8.2:+38372159 | None:intergenic |
| ACTCGGACGTGTTCAACCTC+AGG | 0.538636 | 8.2:+38372256 | MS.gene25293:CDS |
| GAAATGATGAAGGGATGGGA+TGG | 0.548556 | 8.2:+38372158 | None:intergenic |
| TGAAGAAATGATGAAGGGAT+GGG | 0.608006 | 8.2:+38372154 | None:intergenic |
| AGGAACTCGATGGCTGATCG+TGG | 0.610120 | 8.2:-38372219 | None:intergenic |
| TAATTCATGTTGTTCCGTTG+AGG | 0.612857 | 8.2:+38372381 | MS.gene25293:CDS |
| GAAATTGAAGAAATGATGAA+GGG | 0.630442 | 8.2:+38372149 | None:intergenic |
| CCGAGTCGAACCGATTGAGA+AGG | 0.642055 | 8.2:-38372239 | None:intergenic |
| AAACACTAAAATCACCTCAA+CGG | 0.644366 | 8.2:-38372395 | None:intergenic |
| CCGATTGAGAAGGAACTCGA+TGG | 0.660768 | 8.2:-38372229 | None:intergenic |
| GTGCGATAAGAAGGAGCCTG+AGG | 0.698477 | 8.2:-38372272 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAATTTTCCTGTTCGATTCA+AGG | + | chr8.2:38372319-38372338 | MS.gene25293:CDS | 30.0% |
| CAGAAAATAGTGCGATAAGA+AGG | - | chr8.2:38372284-38372303 | None:intergenic | 35.0% | |
| TAATTCATGTTGTTCCGTTG+AGG | + | chr8.2:38372381-38372400 | MS.gene25293:CDS | 35.0% | |
| AAACGAACCTTGAATCGAAC+AGG | - | chr8.2:38372329-38372348 | None:intergenic | 40.0% | |
| CCATCGAGTTCCTTCTCAAT+CGG | + | chr8.2:38372229-38372248 | MS.gene25293:CDS | 45.0% | |
| CCGATTGAGAAGGAACTCGA+TGG | - | chr8.2:38372232-38372251 | None:intergenic | 50.0% | |
| !! | CCTTCTCAATCGGTTCGACT+CGG | + | chr8.2:38372239-38372258 | MS.gene25293:CDS | 50.0% |
| ACTCGGACGTGTTCAACCTC+AGG | + | chr8.2:38372256-38372275 | MS.gene25293:CDS | 55.0% | |
| AGGAACTCGATGGCTGATCG+TGG | - | chr8.2:38372222-38372241 | None:intergenic | 55.0% | |
| CCGAGTCGAACCGATTGAGA+AGG | - | chr8.2:38372242-38372261 | None:intergenic | 55.0% | |
| GTGCGATAAGAAGGAGCCTG+AGG | - | chr8.2:38372275-38372294 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 38372161 | 38372406 | 38372161 | ID=MS.gene25293 |
| chr8.2 | mRNA | 38372161 | 38372406 | 38372161 | ID=MS.gene25293.t1;Parent=MS.gene25293 |
| chr8.2 | exon | 38372161 | 38372406 | 38372161 | ID=MS.gene25293.t1.exon1;Parent=MS.gene25293.t1 |
| chr8.2 | CDS | 38372161 | 38372406 | 38372161 | ID=cds.MS.gene25293.t1;Parent=MS.gene25293.t1 |
| Gene Sequence |
| Protein sequence |
