Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25349.t1 | PNX85476.1 | 80.4 | 199 | 37 | 1 | 1 | 197 | 211 | 409 | 1.40E-89 | 339.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25349.t1 | Q9M2N5 | 36.7 | 215 | 125 | 4 | 1 | 214 | 491 | 695 | 1.3e-35 | 151.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25349.t1 | A0A2K3M3W2 | 80.4 | 199 | 37 | 1 | 1 | 197 | 211 | 409 | 9.9e-90 | 339.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene25349 | 0.813449 | 2.55E-51 | -1.69E-46 |
| MS.gene049711 | MS.gene25349 | 0.812978 | 3.23E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene25349 | 0.824511 | 7.76E-54 | -1.69E-46 |
| MS.gene050559 | MS.gene25349 | 0.800226 | 1.59E-48 | -1.69E-46 |
| MS.gene050561 | MS.gene25349 | 0.800771 | 1.23E-48 | -1.69E-46 |
| MS.gene050562 | MS.gene25349 | 0.800037 | 1.74E-48 | -1.69E-46 |
| MS.gene051247 | MS.gene25349 | 0.802617 | 5.15E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene25349 | 0.837523 | 4.94E-57 | -1.69E-46 |
| MS.gene051594 | MS.gene25349 | 0.803037 | 4.22E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene25349 | 0.81606 | 6.72E-52 | -1.69E-46 |
| MS.gene051720 | MS.gene25349 | 0.800257 | 1.57E-48 | -1.69E-46 |
| MS.gene051744 | MS.gene25349 | 0.837691 | 4.48E-57 | -1.69E-46 |
| MS.gene052463 | MS.gene25349 | 0.801558 | 8.51E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene25349 | 0.827887 | 1.22E-54 | -1.69E-46 |
| MS.gene052582 | MS.gene25349 | 0.826759 | 2.27E-54 | -1.69E-46 |
| MS.gene052584 | MS.gene25349 | 0.821546 | 3.81E-53 | -1.69E-46 |
| MS.gene052586 | MS.gene25349 | 0.802084 | 6.64E-49 | -1.69E-46 |
| MS.gene052773 | MS.gene25349 | 0.800925 | 1.15E-48 | -1.69E-46 |
| MS.gene053226 | MS.gene25349 | 0.805138 | 1.54E-49 | -1.69E-46 |
| MS.gene05365 | MS.gene25349 | 0.813305 | 2.74E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene25349 | 0.811456 | 6.94E-51 | -1.69E-46 |
| MS.gene05423 | MS.gene25349 | 0.818268 | 2.14E-52 | -1.69E-46 |
| MS.gene054651 | MS.gene25349 | 0.845768 | 3.31E-59 | -1.69E-46 |
| MS.gene054775 | MS.gene25349 | 0.817298 | 3.55E-52 | -1.69E-46 |
| MS.gene055228 | MS.gene25349 | 0.803733 | 3.03E-49 | -1.69E-46 |
| MS.gene055586 | MS.gene25349 | 0.802301 | 5.99E-49 | -1.69E-46 |
| MS.gene055778 | MS.gene25349 | 0.803204 | 3.90E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene25349 | 0.805228 | 1.48E-49 | -1.69E-46 |
| MS.gene056366 | MS.gene25349 | 0.805797 | 1.12E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene25349 | 0.826077 | 3.31E-54 | -1.69E-46 |
| MS.gene05690 | MS.gene25349 | 0.825684 | 4.10E-54 | -1.69E-46 |
| MS.gene057102 | MS.gene25349 | 0.800015 | 1.76E-48 | -1.69E-46 |
| MS.gene057921 | MS.gene25349 | 0.811348 | 7.32E-51 | -1.69E-46 |
| MS.gene058037 | MS.gene25349 | 0.807388 | 5.18E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene25349 | 0.822868 | 1.88E-53 | -1.69E-46 |
| MS.gene059015 | MS.gene25349 | 0.810497 | 1.12E-50 | -1.69E-46 |
| MS.gene059625 | MS.gene25349 | 0.857173 | 1.95E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene25349.t1 | AT3G42170 | 38.021 | 192 | 110 | 3 | 1 | 191 | 491 | 674 | 6.26e-41 | 148 |
| MS.gene25349.t1 | AT3G42170 | 38.021 | 192 | 110 | 3 | 1 | 191 | 469 | 652 | 6.55e-41 | 148 |
| MS.gene25349.t1 | AT1G18560 | 27.861 | 201 | 116 | 7 | 2 | 191 | 467 | 649 | 3.95e-13 | 68.6 |
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCAGCTTCACTCTTCAAAA+TGG | 0.170935 | 8.2:-39029159 | None:intergenic |
| AACGGAGTTGGATCATTATC+TGG | 0.259746 | 8.2:+39028599 | MS.gene25349:CDS |
| TGCAGAAATTTGATAAATAT+TGG | 0.280085 | 8.2:+39028295 | MS.gene25349:CDS |
| CCACATCAGAATATCAAAAT+TGG | 0.289304 | 8.2:-39028646 | None:intergenic |
| TTTGGATCCAAGATACAAAA+TGG | 0.298966 | 8.2:+39028353 | MS.gene25349:CDS |
| GATGGGAGTTGCTACTGTTT+TGG | 0.320873 | 8.2:+39028335 | MS.gene25349:CDS |
| TCAGAAGCTACTGAAGTAAT+AGG | 0.323940 | 8.2:-39028732 | None:intergenic |
| ATATCAAAATTGGCACTTCT+AGG | 0.340733 | 8.2:-39028636 | None:intergenic |
| TTTGCAATTTCTTGCAATGT+TGG | 0.382104 | 8.2:-39028693 | None:intergenic |
| AAGATCTTACATTCATCATC+AGG | 0.417940 | 8.2:-39029030 | None:intergenic |
| TCAGCTTCACTCTTCAAAAT+GGG | 0.432393 | 8.2:-39029158 | None:intergenic |
| TAGCAACTCCCATCATATCC+TGG | 0.454437 | 8.2:-39028326 | None:intergenic |
| GCTAATTCTGTGGCTAATGA+TGG | 0.459373 | 8.2:+39028516 | MS.gene25349:CDS |
| ACTCTGTTGAGCAAGTTAAG+AGG | 0.474739 | 8.2:+39028415 | MS.gene25349:CDS |
| GAGTGTCACCCAGGATATGA+TGG | 0.476745 | 8.2:+39028317 | MS.gene25349:CDS |
| CTCATCGTAGTCGACTTCAT+TGG | 0.485204 | 8.2:+39028790 | MS.gene25349:CDS |
| ATATGGAGCAAGGAGAATAA+TGG | 0.491041 | 8.2:+39028849 | MS.gene25349:CDS |
| TAGAACATCATTATATAAAA+CGG | 0.495038 | 8.2:+39028581 | MS.gene25349:CDS |
| ATCATTATATAAAACGGAGT+TGG | 0.499119 | 8.2:+39028587 | MS.gene25349:CDS |
| TAAAGAAATGGCTAAGAAGA+TGG | 0.502305 | 8.2:+39028272 | None:intergenic |
| CTTTGATGTGTGCTAGAAGT+TGG | 0.508597 | 8.2:+39028826 | MS.gene25349:CDS |
| ACTTCATTGGACAACCTTGG+AGG | 0.521822 | 8.2:+39028803 | MS.gene25349:CDS |
| GAATCAGCATTCAGTACAAG+TGG | 0.537506 | 8.2:+39028753 | MS.gene25349:CDS |
| TCGACTTCATTGGACAACCT+TGG | 0.544964 | 8.2:+39028800 | MS.gene25349:CDS |
| AGCAACTCCCATCATATCCT+GGG | 0.551068 | 8.2:-39028325 | None:intergenic |
| TGTGTGCTAGAAGTTGGATA+TGG | 0.560174 | 8.2:+39028832 | MS.gene25349:CDS |
| TAAATATTGGAGTGTCACCC+AGG | 0.565226 | 8.2:+39028308 | MS.gene25349:CDS |
| ACGGAGTTGGATCATTATCT+GGG | 0.571432 | 8.2:+39028600 | MS.gene25349:CDS |
| CTGATGTGGTGGAAGTTGAA+TGG | 0.578791 | 8.2:+39028660 | MS.gene25349:CDS |
| ATTATGATGCATATGTCAAG+AGG | 0.590157 | 8.2:+39028547 | MS.gene25349:CDS |
| AAGTTCAATCAAGACACTTG+TGG | 0.595420 | 8.2:+39028480 | MS.gene25349:CDS |
| AGACACTTGTGGTGAATCGT+CGG | 0.609844 | 8.2:+39028491 | MS.gene25349:CDS |
| AGTGTCACCCAGGATATGAT+GGG | 0.628166 | 8.2:+39028318 | MS.gene25349:CDS |
| AGCACACATCAAAGCCTCCA+AGG | 0.630649 | 8.2:-39028817 | None:intergenic |
| TAGAAGTTGGATATGGAGCA+AGG | 0.637735 | 8.2:+39028839 | MS.gene25349:CDS |
| ATAGGTGAAATATTGACTAG+TGG | 0.646439 | 8.2:+39029127 | MS.gene25349:intron |
| ATCGTCGGAAGCTAATTCTG+TGG | 0.696219 | 8.2:+39028506 | MS.gene25349:CDS |
| CAATGAAGTCGACTACGATG+AGG | 0.702001 | 8.2:-39028789 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTGTATTTTTTATTATAT+AGG | + | chr8.2:39029109-39029128 | MS.gene25349:intron | 10.0% |
| !! | AAACATTAAAGTTAGTAAAT+TGG | - | chr8.2:39029081-39029100 | None:intergenic | 15.0% |
| !! | TAGAACATCATTATATAAAA+CGG | + | chr8.2:39028581-39028600 | MS.gene25349:CDS | 15.0% |
| !!! | TACTATTTTGAAAAATTGTA+TGG | + | chr8.2:39028387-39028406 | MS.gene25349:CDS | 15.0% |
| !! | ATTAAAGTTAGTAAATTGGA+AGG | - | chr8.2:39029077-39029096 | None:intergenic | 20.0% |
| !! | CTTTCAATTTACATATCTTA+AGG | + | chr8.2:39028889-39028908 | MS.gene25349:intron | 20.0% |
| !! | TGCAGAAATTTGATAAATAT+TGG | + | chr8.2:39028295-39028314 | MS.gene25349:CDS | 20.0% |
| ! | ATCATTATATAAAACGGAGT+TGG | + | chr8.2:39028587-39028606 | MS.gene25349:CDS | 25.0% |
| ! | GGAAGGTAGTATTAAATAAA+TGG | - | chr8.2:39029060-39029079 | None:intergenic | 25.0% |
| AAGATCTTACATTCATCATC+AGG | - | chr8.2:39029033-39029052 | None:intergenic | 30.0% | |
| ACTGCATTTGCATTATTTAC+AGG | + | chr8.2:39028952-39028971 | MS.gene25349:intron | 30.0% | |
| ATATCAAAATTGGCACTTCT+AGG | - | chr8.2:39028639-39028658 | None:intergenic | 30.0% | |
| ATTATGATGCATATGTCAAG+AGG | + | chr8.2:39028547-39028566 | MS.gene25349:CDS | 30.0% | |
| CCACATCAGAATATCAAAAT+TGG | - | chr8.2:39028649-39028668 | None:intergenic | 30.0% | |
| TTTGCAATTTCTTGCAATGT+TGG | - | chr8.2:39028696-39028715 | None:intergenic | 30.0% | |
| TTTGGATCCAAGATACAAAA+TGG | + | chr8.2:39028353-39028372 | MS.gene25349:CDS | 30.0% | |
| ! | ATAGGTGAAATATTGACTAG+TGG | + | chr8.2:39029127-39029146 | MS.gene25349:intron | 30.0% |
| !! | ATTTTGATATTCTGATGTGG+TGG | + | chr8.2:39028649-39028668 | MS.gene25349:CDS | 30.0% |
| !! | CCAATTTTGATATTCTGATG+TGG | + | chr8.2:39028646-39028665 | MS.gene25349:CDS | 30.0% |
| AAGTTCAATCAAGACACTTG+TGG | + | chr8.2:39028480-39028499 | MS.gene25349:CDS | 35.0% | |
| ATATGGAGCAAGGAGAATAA+TGG | + | chr8.2:39028849-39028868 | MS.gene25349:CDS | 35.0% | |
| TCAGAAGCTACTGAAGTAAT+AGG | - | chr8.2:39028735-39028754 | None:intergenic | 35.0% | |
| ! | AGCAACTCCATTTTGTATCT+TGG | - | chr8.2:39028363-39028382 | None:intergenic | 35.0% |
| AACGGAGTTGGATCATTATC+TGG | + | chr8.2:39028599-39028618 | MS.gene25349:CDS | 40.0% | |
| ACGGAGTTGGATCATTATCT+GGG | + | chr8.2:39028600-39028619 | MS.gene25349:CDS | 40.0% | |
| ACTCTGTTGAGCAAGTTAAG+AGG | + | chr8.2:39028415-39028434 | MS.gene25349:CDS | 40.0% | |
| CTTTGATGTGTGCTAGAAGT+TGG | + | chr8.2:39028826-39028845 | MS.gene25349:CDS | 40.0% | |
| GAATCAGCATTCAGTACAAG+TGG | + | chr8.2:39028753-39028772 | MS.gene25349:CDS | 40.0% | |
| GCTAATTCTGTGGCTAATGA+TGG | + | chr8.2:39028516-39028535 | MS.gene25349:CDS | 40.0% | |
| TAGAAGTTGGATATGGAGCA+AGG | + | chr8.2:39028839-39028858 | MS.gene25349:CDS | 40.0% | |
| TGTGTGCTAGAAGTTGGATA+TGG | + | chr8.2:39028832-39028851 | MS.gene25349:CDS | 40.0% | |
| ! | TAAATATTGGAGTGTCACCC+AGG | + | chr8.2:39028308-39028327 | MS.gene25349:CDS | 40.0% |
| ACTTCATTGGACAACCTTGG+AGG | + | chr8.2:39028803-39028822 | MS.gene25349:CDS | 45.0% | |
| AGACACTTGTGGTGAATCGT+CGG | + | chr8.2:39028491-39028510 | MS.gene25349:CDS | 45.0% | |
| AGCAACTCCCATCATATCCT+GGG | - | chr8.2:39028328-39028347 | None:intergenic | 45.0% | |
| AGTGTCACCCAGGATATGAT+GGG | + | chr8.2:39028318-39028337 | MS.gene25349:CDS | 45.0% | |
| ATCGTCGGAAGCTAATTCTG+TGG | + | chr8.2:39028506-39028525 | MS.gene25349:CDS | 45.0% | |
| CAATGAAGTCGACTACGATG+AGG | - | chr8.2:39028792-39028811 | None:intergenic | 45.0% | |
| TAGCAACTCCCATCATATCC+TGG | - | chr8.2:39028329-39028348 | None:intergenic | 45.0% | |
| TCGACTTCATTGGACAACCT+TGG | + | chr8.2:39028800-39028819 | MS.gene25349:CDS | 45.0% | |
| ! | CTCATCGTAGTCGACTTCAT+TGG | + | chr8.2:39028790-39028809 | MS.gene25349:CDS | 45.0% |
| ! | CTGATGTGGTGGAAGTTGAA+TGG | + | chr8.2:39028660-39028679 | MS.gene25349:CDS | 45.0% |
| ! | GATGGGAGTTGCTACTGTTT+TGG | + | chr8.2:39028335-39028354 | MS.gene25349:CDS | 45.0% |
| AGCACACATCAAAGCCTCCA+AGG | - | chr8.2:39028820-39028839 | None:intergenic | 50.0% | |
| GAGTGTCACCCAGGATATGA+TGG | + | chr8.2:39028317-39028336 | MS.gene25349:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 39028279 | 39029174 | 39028279 | ID=MS.gene25349 |
| chr8.2 | mRNA | 39028279 | 39029174 | 39028279 | ID=MS.gene25349.t1;Parent=MS.gene25349 |
| chr8.2 | exon | 39028279 | 39028870 | 39028279 | ID=MS.gene25349.t1.exon1;Parent=MS.gene25349.t1 |
| chr8.2 | CDS | 39028279 | 39028870 | 39028279 | ID=cds.MS.gene25349.t1;Parent=MS.gene25349.t1 |
| chr8.2 | exon | 39028974 | 39029042 | 39028974 | ID=MS.gene25349.t1.exon2;Parent=MS.gene25349.t1 |
| chr8.2 | CDS | 39028974 | 39029042 | 39028974 | ID=cds.MS.gene25349.t1;Parent=MS.gene25349.t1 |
| chr8.2 | exon | 39029131 | 39029174 | 39029131 | ID=MS.gene25349.t1.exon3;Parent=MS.gene25349.t1 |
| chr8.2 | CDS | 39029131 | 39029174 | 39029131 | ID=cds.MS.gene25349.t1;Parent=MS.gene25349.t1 |
| Gene Sequence |
| Protein sequence |
