AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene057921


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057921.t1 MTR_3g023760 88.912 478 29 7 1 464 1 468 0.0 744
MS.gene057921.t1 MTR_4g109340 39.871 464 196 20 1 427 1 418 1.01e-75 246
MS.gene057921.t1 MTR_4g109340 33.742 163 71 5 309 460 380 516 6.36e-16 80.5
MS.gene057921.t1 MTR_5g043930 32.400 250 113 7 11 223 5 235 1.72e-22 100
MS.gene057921.t1 MTR_4g082980 35.971 139 59 4 1 123 1 125 3.91e-15 77.8
MS.gene057921.t1 MTR_4g028610 25.210 476 255 20 5 459 8 403 7.31e-15 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene057921.t1 AT1G26610 39.474 266 125 12 1 258 1 238 5.86e-31 124
MS.gene057921.t1 AT1G26590 28.509 228 108 7 1 223 1 178 1.65e-13 72.0
MS.gene057921.t1 AT1G26590 28.509 228 108 7 1 223 1 178 1.65e-13 72.0
MS.gene057921.t1 AT1G26590 28.509 228 108 7 1 223 1 178 1.65e-13 72.0
MS.gene057921.t1 AT1G26590 28.509 228 108 7 1 223 19 196 2.72e-13 71.6

Find 63 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACCAAAGAAGTTGGATTT+TGG 0.178546 3.3:-15034581 MS.gene057921:CDS
CCTTGTTCTTAACTTTCTTT+TGG 0.193235 3.3:+15034898 None:intergenic
TCAGAAACTAAATGAGTTAA+TGG 0.244636 3.3:+15034659 None:intergenic
TTGCCAAAATCCAACTTCTT+TGG 0.270716 3.3:+15034578 None:intergenic
TTGGATCTTCTCTTTCTCTT+TGG 0.274333 3.3:+15034917 None:intergenic
AATGATCAAAGCAGTGAAAA+TGG 0.281007 3.3:-15034200 MS.gene057921:CDS
TAATCTTCCTGCTGCAAGTA+TGG 0.286475 3.3:-15034081 MS.gene057921:CDS
TTTCCTTCTGGTAGATCATT+AGG 0.296213 3.3:-15035301 MS.gene057921:CDS
TGTTGTTGATTCTGAAACTA+AGG 0.321396 3.3:-15034372 MS.gene057921:CDS
AAAGGATGAGAAGAAGAAAA+TGG 0.333136 3.3:-15034432 MS.gene057921:CDS
TAATAATGATGATGATTCTT+TGG 0.335313 3.3:-15034693 MS.gene057921:CDS
TGATGATGATTCTTTGGAAT+TGG 0.360523 3.3:-15034687 MS.gene057921:CDS
TGATTCTGATTTAGCATTTG+AGG 0.378036 3.3:-15034399 MS.gene057921:CDS
AGAGTTTCTGTTGTTGAGAA+TGG 0.378659 3.3:-15034455 MS.gene057921:CDS
AAATGGAAACAGTACTGAAT+TGG 0.379398 3.3:-15034183 MS.gene057921:CDS
ATGTCCTCCAAGAGCTTGAT+AGG 0.380591 3.3:+15034265 None:intergenic
TTACATCAGAAGGTGAATTC+AGG 0.392222 3.3:-15034349 MS.gene057921:CDS
AAACAGTACTGAATTGGAAT+TGG 0.395453 3.3:-15034177 MS.gene057921:CDS
CAATCTATGAAAGCTTTGTT+TGG 0.400642 3.3:-15035025 MS.gene057921:CDS
TTTCAATCCTATCAAGCTCT+TGG 0.409327 3.3:-15034272 MS.gene057921:CDS
TGTGTAAAGAATGTGGTAAA+GGG 0.417853 3.3:-15035051 MS.gene057921:CDS
ATGAAGAAGAAAGAAGGTTA+TGG 0.434835 3.3:-15035169 MS.gene057921:CDS
TTCTGATAATAATGATGAAA+AGG 0.437329 3.3:-15034804 MS.gene057921:CDS
CATTTAGTTTCTGAGATTGA+AGG 0.443179 3.3:-15034650 MS.gene057921:CDS
AGTAGATGCTGAGGGAAGAA+TGG 0.451227 3.3:-15034153 MS.gene057921:CDS
TTGTGTAAAGAATGTGGTAA+AGG 0.465384 3.3:-15035052 MS.gene057921:CDS
TTATCCAAAACAACATCAAA+AGG 0.478081 3.3:+15035076 None:intergenic
TCTTGAAGCAGAAAACCCTT+TGG 0.484015 3.3:+15034223 None:intergenic
TACAAACCATGGTGGTTAGT+TGG 0.485401 3.3:-15034029 MS.gene057921:CDS
GTTCAGATGTTAAGTCCAAA+GGG 0.490833 3.3:-15034547 MS.gene057921:CDS
GTGAAAGAGTTGCTGAAATG+AGG 0.492259 3.3:-15034124 MS.gene057921:CDS
AGAAGAATGAAGAAGAAAGA+AGG 0.493856 3.3:-15035175 MS.gene057921:CDS
GACATAGAGCTAGTCATAAG+AGG 0.494451 3.3:-15034247 MS.gene057921:CDS
CAATCCTATCAAGCTCTTGG+AGG 0.503614 3.3:-15034269 MS.gene057921:CDS
TGTTCAGATGTTAAGTCCAA+AGG 0.512057 3.3:-15034548 MS.gene057921:CDS
TGAAAGAGTTGCTGAAATGA+GGG 0.515667 3.3:-15034123 MS.gene057921:CDS
TCATCATCCATACTTGCAGC+AGG 0.519738 3.3:+15034074 None:intergenic
GCTAGTCATAAGAGGACCAA+AGG 0.524798 3.3:-15034239 MS.gene057921:CDS
AGAGATGTAAGACCTTGGTT+TGG 0.525051 3.3:-15034743 MS.gene057921:CDS
AAGAGAAAATATCAGAAAAG+AGG 0.527607 3.3:-15034326 MS.gene057921:CDS
ATTTAGTTTCTGAGATTGAA+GGG 0.531202 3.3:-15034649 MS.gene057921:CDS
CTAGTCATAAGAGGACCAAA+GGG 0.538026 3.3:-15034238 MS.gene057921:CDS
GAGAGTGAAGGAAATGATGA+TGG 0.545086 3.3:-15034500 MS.gene057921:CDS
ATCAAGCAAGAGGCAATGAT+AGG 0.560531 3.3:-15033999 MS.gene057921:CDS
CCTTCTGGTAGATCATTAGG+TGG 0.567747 3.3:-15035298 MS.gene057921:CDS
GATAAATTGTGTAAAGAATG+TGG 0.571592 3.3:-15035058 MS.gene057921:CDS
GAAACTAAGGTTACATCAGA+AGG 0.573783 3.3:-15034359 MS.gene057921:CDS
TGATGAAAAGGAACAAGAAG+AGG 0.575044 3.3:-15034792 MS.gene057921:CDS
ATTCTGAGATTTACAAACCA+TGG 0.580716 3.3:-15034040 MS.gene057921:CDS
GGAATTGGAAGTAGATGCTG+AGG 0.586355 3.3:-15034162 MS.gene057921:CDS
TATGATCATGATGATGATGA+TGG 0.588201 3.3:-15035223 MS.gene057921:CDS
TTAGTAGAGATGTAAGACCT+TGG 0.592699 3.3:-15034748 MS.gene057921:CDS
GAGAGAATCCAAAGAAAACA+TGG 0.594959 3.3:-15035141 MS.gene057921:CDS
TTCTGTTGTTGAGAATGGAA+AGG 0.607290 3.3:-15034450 MS.gene057921:CDS
AGTATGGATGATGAAAGAAA+TGG 0.608964 3.3:-15034065 MS.gene057921:CDS
TGGAAGCAGTATCAAGCAAG+AGG 0.613537 3.3:-15034009 MS.gene057921:CDS
GATCATTAGGTGGTCACATG+AGG 0.626071 3.3:-15035288 MS.gene057921:CDS
CCAAAAGAAAGTTAAGAACA+AGG 0.628452 3.3:-15034898 MS.gene057921:CDS
CTGAGATTTACAAACCATGG+TGG 0.652285 3.3:-15034037 MS.gene057921:CDS
CCACCTAATGATCTACCAGA+AGG 0.655089 3.3:+15035298 None:intergenic
GAATTGGAAGTAGATGCTGA+GGG 0.674106 3.3:-15034161 MS.gene057921:CDS
CTGCTTCCAACTAACCACCA+TGG 0.679286 3.3:+15034023 None:intergenic
ATAGAATTGATACCAAACCA+AGG 0.704170 3.3:+15034731 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTGATAATAATGATGAAA+AGG - chr3.3:15034534-15034553 MS.gene057921:CDS 20.0%
!!! TAATAATGATGATGATTCTT+TGG - chr3.3:15034645-15034664 MS.gene057921:CDS 20.0%
! AAGAGAAAATATCAGAAAAG+AGG - chr3.3:15035012-15035031 MS.gene057921:CDS 25.0%
! GATAAATTGTGTAAAGAATG+TGG - chr3.3:15034280-15034299 MS.gene057921:CDS 25.0%
! TCAGAAACTAAATGAGTTAA+TGG + chr3.3:15034682-15034701 None:intergenic 25.0%
! TTATCCAAAACAACATCAAA+AGG + chr3.3:15034265-15034284 None:intergenic 25.0%
!!! ATTTAGTTTCTGAGATTGAA+GGG - chr3.3:15034689-15034708 MS.gene057921:CDS 25.0%
!!! GAAGAATAAGAGTTTTGAAT+TGG - chr3.3:15034354-15034373 MS.gene057921:CDS 25.0%
AAAGGATGAGAAGAAGAAAA+TGG - chr3.3:15034906-15034925 MS.gene057921:CDS 30.0%
AATGATCAAAGCAGTGAAAA+TGG - chr3.3:15035138-15035157 MS.gene057921:CDS 30.0%
AGAAGAATGAAGAAGAAAGA+AGG - chr3.3:15034163-15034182 MS.gene057921:CDS 30.0%
AGTATGGATGATGAAAGAAA+TGG - chr3.3:15035273-15035292 MS.gene057921:CDS 30.0%
ATAGAATTGATACCAAACCA+AGG + chr3.3:15034610-15034629 None:intergenic 30.0%
ATGAAGAAGAAAGAAGGTTA+TGG - chr3.3:15034169-15034188 MS.gene057921:CDS 30.0%
CCAAAAGAAAGTTAAGAACA+AGG - chr3.3:15034440-15034459 MS.gene057921:CDS 30.0%
GAACAAAAAACCAAAGAAGT+TGG - chr3.3:15034750-15034769 MS.gene057921:CDS 30.0%
TGATTCTGATTTAGCATTTG+AGG - chr3.3:15034939-15034958 MS.gene057921:CDS 30.0%
TGTGTAAAGAATGTGGTAAA+GGG - chr3.3:15034287-15034306 MS.gene057921:CDS 30.0%
TTGTGTAAAGAATGTGGTAA+AGG - chr3.3:15034286-15034305 MS.gene057921:CDS 30.0%
! AAACAGTACTGAATTGGAAT+TGG - chr3.3:15035161-15035180 MS.gene057921:CDS 30.0%
! AAACCAAAGAAGTTGGATTT+TGG - chr3.3:15034757-15034776 MS.gene057921:CDS 30.0%
! AAATGGAAACAGTACTGAAT+TGG - chr3.3:15035155-15035174 MS.gene057921:CDS 30.0%
! AACTTCTAAACTTTTCCCTT+TGG + chr3.3:15034809-15034828 None:intergenic 30.0%
! CCTTGTTCTTAACTTTCTTT+TGG + chr3.3:15034443-15034462 None:intergenic 30.0%
! TATGATCATGATGATGATGA+TGG - chr3.3:15034115-15034134 MS.gene057921:CDS 30.0%
!! AGATCCTTTTGATGTTGTTT+TGG - chr3.3:15034258-15034277 MS.gene057921:CDS 30.0%
!! ATTCTGAGATTTACAAACCA+TGG - chr3.3:15035298-15035317 MS.gene057921:CDS 30.0%
!! CAATCTATGAAAGCTTTGTT+TGG - chr3.3:15034313-15034332 MS.gene057921:CDS 30.0%
!! CATTTAGTTTCTGAGATTGA+AGG - chr3.3:15034688-15034707 MS.gene057921:CDS 30.0%
!! TGATGATGATTCTTTGGAAT+TGG - chr3.3:15034651-15034670 MS.gene057921:CDS 30.0%
!! TGTTGTTGATTCTGAAACTA+AGG - chr3.3:15034966-15034985 MS.gene057921:CDS 30.0%
!!! GTTTTTGATAATGAGAGTGA+AGG - chr3.3:15034826-15034845 MS.gene057921:CDS 30.0%
AGAGTTTCTGTTGTTGAGAA+TGG - chr3.3:15034883-15034902 MS.gene057921:CDS 35.0%
GAAACTAAGGTTACATCAGA+AGG - chr3.3:15034979-15034998 MS.gene057921:CDS 35.0%
GAGAGAATCCAAAGAAAACA+TGG - chr3.3:15034197-15034216 MS.gene057921:CDS 35.0%
GTTCAGATGTTAAGTCCAAA+GGG - chr3.3:15034791-15034810 MS.gene057921:CDS 35.0%
TGAAAGAGTTGCTGAAATGA+GGG - chr3.3:15035215-15035234 MS.gene057921:CDS 35.0%
TGATGAAAAGGAACAAGAAG+AGG - chr3.3:15034546-15034565 MS.gene057921:CDS 35.0%
TGTTCAGATGTTAAGTCCAA+AGG - chr3.3:15034790-15034809 MS.gene057921:CDS 35.0%
TTACATCAGAAGGTGAATTC+AGG - chr3.3:15034989-15035008 MS.gene057921:CDS 35.0%
TTAGTAGAGATGTAAGACCT+TGG - chr3.3:15034590-15034609 MS.gene057921:CDS 35.0%
TTCTGTTGTTGAGAATGGAA+AGG - chr3.3:15034888-15034907 MS.gene057921:CDS 35.0%
TTGCCAAAATCCAACTTCTT+TGG + chr3.3:15034763-15034782 None:intergenic 35.0%
TTGGATCTTCTCTTTCTCTT+TGG + chr3.3:15034424-15034443 None:intergenic 35.0%
TTTCAATCCTATCAAGCTCT+TGG - chr3.3:15035066-15035085 MS.gene057921:CDS 35.0%
TTTCCTTCTGGTAGATCATT+AGG - chr3.3:15034037-15034056 MS.gene057921:CDS 35.0%
! AGTACTCTCCATGTTTTCTT+TGG + chr3.3:15034208-15034227 None:intergenic 35.0%
! TGCAGAAAGAATTTTCCTTC+TGG - chr3.3:15034025-15034044 MS.gene057921:CDS 35.0%
AGAGATGTAAGACCTTGGTT+TGG - chr3.3:15034595-15034614 MS.gene057921:CDS 40.0%
ATCAAGCAAGAGGCAATGAT+AGG - chr3.3:15035339-15035358 MS.gene057921:CDS 40.0%
CTAGTCATAAGAGGACCAAA+GGG - chr3.3:15035100-15035119 MS.gene057921:CDS 40.0%
CTGAGATTTACAAACCATGG+TGG - chr3.3:15035301-15035320 MS.gene057921:CDS 40.0%
GAATTGGAAGTAGATGCTGA+GGG - chr3.3:15035177-15035196 MS.gene057921:CDS 40.0%
GACATAGAGCTAGTCATAAG+AGG - chr3.3:15035091-15035110 MS.gene057921:CDS 40.0%
GAGAGTGAAGGAAATGATGA+TGG - chr3.3:15034838-15034857 MS.gene057921:CDS 40.0%
GTGAAAGAGTTGCTGAAATG+AGG - chr3.3:15035214-15035233 MS.gene057921:CDS 40.0%
TAATCTTCCTGCTGCAAGTA+TGG - chr3.3:15035257-15035276 MS.gene057921:CDS 40.0%
TACAAACCATGGTGGTTAGT+TGG - chr3.3:15035309-15035328 MS.gene057921:CDS 40.0%
TCTTGAAGCAGAAAACCCTT+TGG + chr3.3:15035118-15035137 None:intergenic 40.0%
AGTAGATGCTGAGGGAAGAA+TGG - chr3.3:15035185-15035204 MS.gene057921:CDS 45.0%
ATGTCCTCCAAGAGCTTGAT+AGG + chr3.3:15035076-15035095 None:intergenic 45.0%
CAATCCTATCAAGCTCTTGG+AGG - chr3.3:15035069-15035088 MS.gene057921:CDS 45.0%
CCACCTAATGATCTACCAGA+AGG + chr3.3:15034043-15034062 None:intergenic 45.0%
CCTTCTGGTAGATCATTAGG+TGG - chr3.3:15034040-15034059 MS.gene057921:CDS 45.0%
GATCATTAGGTGGTCACATG+AGG - chr3.3:15034050-15034069 MS.gene057921:CDS 45.0%
GCTAGTCATAAGAGGACCAA+AGG - chr3.3:15035099-15035118 MS.gene057921:CDS 45.0%
GGAATTGGAAGTAGATGCTG+AGG - chr3.3:15035176-15035195 MS.gene057921:CDS 45.0%
TCATCATCCATACTTGCAGC+AGG + chr3.3:15035267-15035286 None:intergenic 45.0%
TGGAAGCAGTATCAAGCAAG+AGG - chr3.3:15035329-15035348 MS.gene057921:CDS 45.0%
CTGCTTCCAACTAACCACCA+TGG + chr3.3:15035318-15035337 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 15033983 15035377 15033983 ID=MS.gene057921
chr3.3 mRNA 15033983 15035377 15033983 ID=MS.gene057921.t1;Parent=MS.gene057921
chr3.3 exon 15033983 15035377 15033983 ID=MS.gene057921.t1.exon1;Parent=MS.gene057921.t1
chr3.3 CDS 15033983 15035377 15033983 ID=cds.MS.gene057921.t1;Parent=MS.gene057921.t1
Gene Sequence

>MS.gene057921

ATGGAAGAAGATCAAGAAGTGATGAAACATGTGTGCAAATTCTGCAGAAAGAATTTTCCTTCTGGTAGATCATTAGGTGGTCACATGAGGTCTCATGATCATGATCATGATCATGATCATGATCATGATGATTATGATCATGATGATGATGATGGTGATGAGTTTGTTGTTGCTGTAAGAAGAAGAATGAAGAAGAAAGAAGGTTATGGTCTTAGAGAGAATCCAAAGAAAACATGGAGAGTACTCACTGATTCAACTAATGAACAAGAGATTCAAGATCCTTTTGATGTTGTTTTGGATAAATTGTGTAAAGAATGTGGTAAAGGGTTTCAATCTATGAAAGCTTTGTTTGGTCACATGAAGTGTCATAAGAAGAATAAGAGTTTTGAATTGGATGAAGAAGAAGAAGAAGATGATGATTCTAATGCAACTGTTCTTCCAAAGAGAAAGAGAAGATCCAAAAGAAAGTTAAGAACAAGGTACAATAAGAGTAATACTTCTTCTTCTGTTGTGAATTTTGATGATAATTCTTTAGATTCTATTGCTGAATCTTCTGATAATAATGATGAAAAGGAACAAGAAGAGGTTGCTATGAGTTTGATCATGCTTAGTAGAGATGTAAGACCTTGGTTTGGTATCAATTCTATTGCTGAATTTTCTGATAATAATGATGATGATTCTTTGGAATTGGAAGCGCCATTAACTCATTTAGTTTCTGAGATTGAAGGGAAAAAGTTTATCACTAATAGTTCTGAAATTGTGAAAATGAACAAAAAACCAAAGAAGTTGGATTTTGGCAATGTTGTTTGTTCAGATGTTAAGTCCAAAGGGAAAAGTTTAGAAGTTTTTGATAATGAGAGTGAAGGAAATGATGATGGATTGAAGATGAAGAAAGCTACTAGAGTTTCTGTTGTTGAGAATGGAAAGGATGAGAAGAAGAAAATGGAGATTGAGCTTGATTCTGATTTAGCATTTGAGGTCAATGTTGTTGATTCTGAAACTAAGGTTACATCAGAAGGTGAATTCAGGAAGAGAAAATATCAGAAAAGAGGAAAATTTGAATGTGCTACTTGCAACAAAAAGTTTCAATCCTATCAAGCTCTTGGAGGACATAGAGCTAGTCATAAGAGGACCAAAGGGTTTTCTGCTTCAAGAAATGATCAAAGCAGTGAAAATGGAAACAGTACTGAATTGGAATTGGAAGTAGATGCTGAGGGAAGAATGGTAGAAAGTGAAAGAGTTGCTGAAATGAGGGATTATCTTGATCTTGATCTTAATCTTCCTGCTGCAAGTATGGATGATGAAAGAAATGGTCATTCTGAGATTTACAAACCATGGTGGTTAGTTGGAAGCAGTATCAAGCAAGAGGCAATGATAGGTCTACTATCAAACTAG

Protein sequence

>MS.gene057921.t1

MEEDQEVMKHVCKFCRKNFPSGRSLGGHMRSHDHDHDHDHDHDDYDHDDDDGDEFVVAVRRRMKKKEGYGLRENPKKTWRVLTDSTNEQEIQDPFDVVLDKLCKECGKGFQSMKALFGHMKCHKKNKSFELDEEEEEDDDSNATVLPKRKRRSKRKLRTRYNKSNTSSSVVNFDDNSLDSIAESSDNNDEKEQEEVAMSLIMLSRDVRPWFGINSIAEFSDNNDDDSLELEAPLTHLVSEIEGKKFITNSSEIVKMNKKPKKLDFGNVVCSDVKSKGKSLEVFDNESEGNDDGLKMKKATRVSVVENGKDEKKKMEIELDSDLAFEVNVVDSETKVTSEGEFRKRKYQKRGKFECATCNKKFQSYQALGGHRASHKRTKGFSASRNDQSSENGNSTELELEVDAEGRMVESERVAEMRDYLDLDLNLPAASMDDERNGHSEIYKPWWLVGSSIKQEAMIGLLSN