AlfalfaGEDB | MS.gene26306

M. sativa cultivar XinJiangDaYe / MS.gene26306

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008168 GO:0008171 GO:0016740 GO:0016741
PFAM: PF00891.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26306.t1	QGL04297.1	96.7	246	8	0	1	246	113	358	6.60E-135	490

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26306.t1	B0ZB57	59.7	248	98	1	1	246	130	377	7.8e-84	311.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26306.t1	G7KFI9	88.7	247	27	1	1	246	95	341	5.7e-120	439.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene05016	MS.gene26306	0.807041	6.14E-50	-1.69E-46
MS.gene050226	MS.gene26306	0.800488	1.41E-48	-1.69E-46
MS.gene050762	MS.gene26306	0.813387	2.63E-51	-1.69E-46
MS.gene050763	MS.gene26306	0.814981	1.17E-51	-1.69E-46
MS.gene050807	MS.gene26306	0.843331	1.50E-58	-1.69E-46
MS.gene050808	MS.gene26306	0.834048	3.76E-56	-1.69E-46
MS.gene050826	MS.gene26306	0.801105	1.05E-48	-1.69E-46
MS.gene050959	MS.gene26306	0.81171	6.11E-51	-1.69E-46
MS.gene052020	MS.gene26306	0.804993	1.65E-49	-1.69E-46
MS.gene052496	MS.gene26306	0.808034	3.78E-50	-1.69E-46
MS.gene052665	MS.gene26306	0.841539	4.47E-58	-1.69E-46
MS.gene053074	MS.gene26306	0.806813	6.86E-50	-1.69E-46
MS.gene053565	MS.gene26306	0.804527	2.07E-49	-1.69E-46
MS.gene054379	MS.gene26306	0.821251	4.46E-53	-1.69E-46
MS.gene054468	MS.gene26306	0.802829	4.66E-49	-1.69E-46
MS.gene055356	MS.gene26306	0.827931	1.19E-54	-1.69E-46
MS.gene055357	MS.gene26306	0.807907	4.02E-50	-1.69E-46
MS.gene055358	MS.gene26306	0.8275	1.51E-54	-1.69E-46
MS.gene055926	MS.gene26306	0.800818	1.21E-48	-1.69E-46
MS.gene056553	MS.gene26306	0.818043	2.41E-52	-1.69E-46
MS.gene057469	MS.gene26306	0.806191	9.27E-50	-1.69E-46
MS.gene057929	MS.gene26306	0.803373	3.60E-49	-1.69E-46
MS.gene058168	MS.gene26306	0.805605	1.23E-49	-1.69E-46
MS.gene059128	MS.gene26306	0.821535	3.83E-53	-1.69E-46
MS.gene059713	MS.gene26306	0.80895	2.41E-50	-1.69E-46
MS.gene059725	MS.gene26306	0.800167	1.64E-48	-1.69E-46
MS.gene060998	MS.gene26306	0.808506	2.99E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26306.t1	MTR_5g074540	88.664	247	27	1	1	246	95	341	9.02e-158	441
MS.gene26306.t1	MTR_5g074600	80.972	247	46	1	1	246	113	359	4.86e-146	412
MS.gene26306.t1	MTR_5g074580	78.543	247	52	1	1	246	114	360	2.63e-141	400
MS.gene26306.t1	MTR_5g074680	78.455	246	52	1	1	245	114	359	1.08e-138	394
MS.gene26306.t1	MTR_5g074610	63.563	247	60	3	1	246	109	326	6.44e-108	314
MS.gene26306.t1	MTR_4g079160	60.484	248	95	3	1	246	109	355	1.09e-102	302
MS.gene26306.t1	MTR_7g014570	46.559	247	128	4	1	246	109	352	1.59e-77	238
MS.gene26306.t1	MTR_4g088200	50.410	244	115	5	7	246	120	361	1.52e-75	233
MS.gene26306.t1	MTR_4g033015	50.403	248	117	5	3	246	118	363	9.55e-73	226
MS.gene26306.t1	MTR_4g033200	50.403	248	117	5	3	246	118	363	1.69e-72	225
MS.gene26306.t1	MTR_4g088340	47.881	236	119	4	13	246	100	333	2.95e-72	224
MS.gene26306.t1	MTR_4g088190	52.459	244	110	5	7	246	123	364	3.41e-72	224
MS.gene26306.t1	MTR_7g014510	42.915	247	137	4	1	246	114	357	3.54e-72	224
MS.gene26306.t1	MTR_4g088345	47.679	237	120	4	12	246	122	356	6.38e-72	224
MS.gene26306.t1	MTR_7g024520	46.610	236	119	4	10	244	119	348	1.56e-70	220
MS.gene26306.t1	MTR_4g033150	48.790	248	121	5	3	246	118	363	5.01e-70	219
MS.gene26306.t1	MTR_1g076940	44.715	246	131	5	3	246	119	361	1.44e-69	218
MS.gene26306.t1	MTR_7g024500	44.351	239	126	4	7	244	116	348	4.61e-69	216
MS.gene26306.t1	MTR_4g033170	48.374	246	123	3	4	246	113	357	2.95e-63	201
MS.gene26306.t1	MTR_4g033085	47.561	246	125	3	4	246	113	357	1.56e-59	192
MS.gene26306.t1	MTR_3g083620	42.241	232	132	2	12	242	127	357	1.01e-56	185
MS.gene26306.t1	MTR_0079s0070	39.357	249	145	5	1	246	89	334	2.77e-51	170
MS.gene26306.t1	MTR_4g038440	39.357	249	145	5	1	246	123	368	9.06e-51	169
MS.gene26306.t1	MTR_0017s0100	38.153	249	148	4	1	246	123	368	1.64e-46	159
MS.gene26306.t1	MTR_1g096310	32.906	234	152	4	13	245	142	371	3.31e-43	150
MS.gene26306.t1	MTR_1g036490	38.000	200	120	3	34	233	160	355	1.33e-40	143
MS.gene26306.t1	MTR_2g055940	35.648	216	129	5	19	231	139	347	1.99e-40	142
MS.gene26306.t1	MTR_8g068690	39.175	194	115	2	34	227	167	357	4.92e-40	142
MS.gene26306.t1	MTR_0591s0010	38.571	210	123	5	19	227	154	358	6.96e-40	141
MS.gene26306.t1	MTR_8g068870	35.514	214	135	2	32	245	152	362	7.86e-40	141
MS.gene26306.t1	MTR_1g036460	37.619	210	127	3	24	233	150	355	1.20e-39	140
MS.gene26306.t1	MTR_7g011990	32.727	220	144	3	13	232	137	352	1.62e-39	140
MS.gene26306.t1	MTR_7g012040	33.182	220	143	3	13	232	138	353	2.13e-39	140
MS.gene26306.t1	MTR_7g118320	37.443	219	127	6	16	231	136	347	2.14e-39	140
MS.gene26306.t1	MTR_7g012070	31.818	220	146	3	13	232	145	360	3.88e-39	139
MS.gene26306.t1	MTR_8g068750	35.211	213	134	3	19	231	154	362	4.29e-39	139
MS.gene26306.t1	MTR_8g024160	34.137	249	152	6	1	245	87	327	7.67e-39	137
MS.gene26306.t1	MTR_1g036510	38.000	200	120	3	34	233	174	369	1.16e-38	138
MS.gene26306.t1	MTR_7g012090	32.727	220	144	3	13	232	145	360	2.77e-38	137
MS.gene26306.t1	MTR_7g011900	33.023	215	140	2	19	233	146	356	3.49e-38	137
MS.gene26306.t1	MTR_7g118300	34.746	236	146	6	1	235	134	362	3.81e-37	134
MS.gene26306.t1	MTR_3g092900	33.937	221	136	6	15	232	136	349	7.80e-37	133
MS.gene26306.t1	MTR_3g021440	33.333	219	141	3	15	233	143	356	1.25e-36	132
MS.gene26306.t1	MTR_5g098170	33.636	220	136	5	17	233	138	350	3.38e-36	131
MS.gene26306.t1	MTR_7g012020	31.481	216	140	4	19	232	145	354	1.44e-35	130
MS.gene26306.t1	MTR_3g021430	31.760	233	150	5	2	232	117	342	1.36e-34	127
MS.gene26306.t1	MTR_6g078850	36.634	202	121	5	32	231	172	368	8.31e-34	127
MS.gene26306.t1	MTR_8g068570	40.361	166	94	4	49	213	156	317	1.14e-31	119
MS.gene26306.t1	MTR_1g076930	38.621	145	87	2	3	146	94	237	1.90e-29	111
MS.gene26306.t1	MTR_8g068730	41.333	150	80	5	85	231	186	330	4.85e-29	112
MS.gene26306.t1	MTR_0794s0020	38.346	133	78	3	3	132	118	249	3.81e-27	105
MS.gene26306.t1	MTR_2g072790	33.333	201	127	5	34	231	143	339	1.38e-25	103
MS.gene26306.t1	MTR_4g038440	35.135	148	94	1	1	146	123	270	5.04e-23	94.7
MS.gene26306.t1	MTR_3g096070	28.241	216	148	4	19	231	109	320	1.62e-22	94.4
MS.gene26306.t1	MTR_1g050220	32.903	155	91	3	82	236	152	293	9.71e-22	91.7
MS.gene26306.t1	MTR_3g096050	27.049	244	158	8	2	231	106	343	2.97e-20	88.6
MS.gene26306.t1	MTR_8g068620	35.398	113	72	1	37	149	2	113	2.31e-19	81.3
MS.gene26306.t1	MTR_7g012020	30.323	155	103	2	19	169	145	298	2.73e-19	85.1
MS.gene26306.t1	MTR_8g068600	32.031	128	85	2	19	146	128	253	2.32e-18	82.4
MS.gene26306.t1	MTR_8g068690	36.207	116	73	1	34	149	167	281	2.42e-18	82.4
MS.gene26306.t1	MTR_1g036510	38.053	113	69	1	34	146	174	285	2.43e-18	82.4
MS.gene26306.t1	MTR_4g025570	42.708	96	51	4	148	242	122	214	2.34e-14	70.1
MS.gene26306.t1	MTR_8g068740	40.789	76	45	0	85	160	186	261	3.82e-14	70.5
MS.gene26306.t1	MTR_7g012020	32.323	99	66	1	19	117	145	242	7.15e-12	63.9
MS.gene26306.t1	MTR_1g036510	37.647	85	52	1	34	118	174	257	1.35e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26306.t1	AT1G51990	35.887	248	151	5	2	246	120	362	5.13e-45	154
MS.gene26306.t1	AT1G51990	35.887	248	151	5	2	246	120	362	5.18e-45	154
MS.gene26306.t1	AT4G35150	39.535	215	122	4	38	245	110	323	6.84e-44	150
MS.gene26306.t1	AT4G35160	36.863	255	149	5	2	245	127	380	1.38e-43	151
MS.gene26306.t1	AT1G77520	33.913	230	143	4	22	245	154	380	2.83e-38	137
MS.gene26306.t1	AT5G54160	34.703	219	137	4	15	232	134	347	2.56e-37	134
MS.gene26306.t1	AT5G53810	36.916	214	131	3	33	245	167	377	1.73e-34	127
MS.gene26306.t1	AT1G63140	39.056	233	133	6	16	245	154	380	9.63e-34	125
MS.gene26306.t1	AT1G63140	39.056	233	133	6	16	245	174	400	1.24e-33	125
MS.gene26306.t1	AT1G77530	35.981	214	133	3	33	245	170	380	2.14e-33	124
MS.gene26306.t1	AT1G62900	38.942	208	121	4	40	245	1	204	1.26e-30	113
MS.gene26306.t1	AT5G37170	33.668	199	127	4	49	245	38	233	1.70e-30	113
MS.gene26306.t1	AT5G37170	33.668	199	127	4	49	245	138	333	1.03e-29	114
MS.gene26306.t1	AT1G33030	32.479	234	149	7	2	232	109	336	1.48e-29	114
MS.gene26306.t1	AT5G37170	34.574	188	118	4	60	245	1	185	1.79e-28	107
MS.gene26306.t1	AT3G53140	39.873	158	85	5	79	231	192	344	8.82e-28	109
MS.gene26306.t1	AT1G21130	34.146	205	122	5	33	232	163	359	6.90e-27	107
MS.gene26306.t1	AT1G21100	33.659	205	123	5	33	232	163	359	2.62e-26	105
MS.gene26306.t1	AT1G21110	33.831	201	120	5	33	228	163	355	6.97e-26	104
MS.gene26306.t1	AT1G21120	33.171	205	124	5	33	232	163	359	3.20e-25	102
MS.gene26306.t1	AT1G21120	33.171	205	124	5	33	232	211	407	6.63e-25	102
MS.gene26306.t1	AT1G76790	32.292	192	118	4	40	227	163	346	7.85e-24	98.6
MS.gene26306.t1	AT1G63140	36.364	132	80	3	16	146	174	302	3.88e-14	70.9
MS.gene26306.t1	AT1G33030	29.452	146	100	3	2	146	109	252	9.31e-11	60.8

Find 52 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTAAATGAGTTGTTAATTT+TGG	0.160023	5.4:+59724126	None:intergenic
ATAGACCTTGATCCTAAAAT+TGG	0.254900	5.4:+59722946	None:intergenic
GATCTCCCACATGTTGTTCA+TGG	0.264137	5.4:-59723941	MS.gene26306:CDS
TGCCATGGATCTATCATATC+TGG	0.330412	5.4:+59724238	None:intergenic
TACAGTCGATATTGCATGAT+TGG	0.335498	5.4:-59723212	MS.gene26306:intron
CAGCACATGAGATGTCGTTT+TGG	0.348362	5.4:-59724168	MS.gene26306:CDS
CTCCATTTGTGGGAATGATT+TGG	0.349338	5.4:+59723976	None:intergenic
CAACCAATGACTCAACTTCA+TGG	0.372843	5.4:+59724038	None:intergenic
AACAAAATTGCATCTGTTGA+CGG	0.377379	5.4:+59723863	None:intergenic
TCGATTAGTTTCCGATTTGT+TGG	0.382710	5.4:-59724083	MS.gene26306:CDS
GAAAACTTAAACTATGTTGC+TGG	0.385861	5.4:-59723905	MS.gene26306:CDS
GTTGATGTTGGAGGAGGTAC+GGG	0.411565	5.4:-59724019	MS.gene26306:CDS
TGCCAAATCATTCCCACAAA+TGG	0.419064	5.4:-59723978	MS.gene26306:CDS
AAGAGAGAAACGAGAAAGAA+TGG	0.424421	5.4:-59723014	MS.gene26306:CDS
ATGGATTAAGTTGATTTCCT+TGG	0.431482	5.4:-59722995	MS.gene26306:CDS
GTTCCATGAAGTTGAGTCAT+TGG	0.432542	5.4:-59724041	MS.gene26306:CDS
CAACTCATTTAACGAATCAA+TGG	0.437712	5.4:-59724116	MS.gene26306:CDS
CATGTTGTTCATGGTTTGCA+AGG	0.449071	5.4:-59723932	MS.gene26306:CDS
AACACAATGCACTCCATTTG+TGG	0.453290	5.4:+59723965	None:intergenic
GCAATATCAAAGAAAGGTAA+AGG	0.460727	5.4:-59723147	MS.gene26306:CDS
GCACTTCTCAACCAACAAAT+CGG	0.467046	5.4:+59724072	None:intergenic
CATGGCATCAATTCTCTGCT+TGG	0.468036	5.4:-59724222	MS.gene26306:CDS
GTTGAGTCATTGGTTGATGT+TGG	0.468409	5.4:-59724031	MS.gene26306:CDS
ATTAAGTTGATTTCCTTGGC+TGG	0.468848	5.4:-59722991	MS.gene26306:CDS
GGTTGATGTTGGAGGAGGTA+CGG	0.474379	5.4:-59724020	MS.gene26306:CDS
ACATGCGGTGCCACACTCAT+TGG	0.507325	5.4:+59724277	None:intergenic
ACACAATGCACTCCATTTGT+GGG	0.515239	5.4:+59723966	None:intergenic
CAATATCAAAGAAAGGTAAA+GGG	0.532814	5.4:-59723146	MS.gene26306:CDS
TTGATGATGGTAGTATTCGC+AGG	0.543043	5.4:-59723039	MS.gene26306:CDS
AAGGATGCAATATCAAAGAA+AGG	0.545671	5.4:-59723153	MS.gene26306:CDS
CTTCTATGACATGTTGATGA+TGG	0.553245	5.4:-59723052	MS.gene26306:CDS
ACGGTGTTAGACAAAGAGAA+TGG	0.563271	5.4:-59723105	MS.gene26306:CDS
GAGTCATTGGTTGATGTTGG+AGG	0.570761	5.4:-59724028	MS.gene26306:CDS
TGCAAACCATGAACAACATG+TGG	0.579903	5.4:+59723935	None:intergenic
AAAGAAAGGTAAAGGGAAGG+TGG	0.580286	5.4:-59723139	MS.gene26306:CDS
GTGTGGCACCGCATGTGCAC+GGG	0.582787	5.4:-59724270	MS.gene26306:CDS
TGGAGGAGGTACGGGGACCA+TGG	0.588947	5.4:-59724011	MS.gene26306:CDS
CAAGCAGAGAATTGATGCCA+TGG	0.591666	5.4:+59724223	None:intergenic
AGTGTGGCACCGCATGTGCA+CGG	0.593144	5.4:-59724271	MS.gene26306:CDS
GTAGTCACTAAAACCAGCCA+AGG	0.595683	5.4:+59722978	None:intergenic
TTGTTGGTTGAGAAGTGCAA+GGG	0.598365	5.4:-59724067	MS.gene26306:CDS
CTGAGTATCCCGTGCACATG+CGG	0.599971	5.4:+59724262	None:intergenic
TTTGTTGGTTGAGAAGTGCA+AGG	0.604264	5.4:-59724068	MS.gene26306:CDS
GTCCAGATATGATAGATCCA+TGG	0.611089	5.4:-59724240	MS.gene26306:CDS
AAAGATATTAAAGAATTGCA+AGG	0.612054	5.4:-59723172	MS.gene26306:CDS
ATCAAAGAAAGGTAAAGGGA+AGG	0.618639	5.4:-59723142	MS.gene26306:CDS
TCATTGGTTGATGTTGGAGG+AGG	0.622074	5.4:-59724025	MS.gene26306:CDS
TTGATGTTGGAGGAGGTACG+GGG	0.638274	5.4:-59724018	MS.gene26306:CDS
TAAGAACAATCCAATGAGTG+TGG	0.663011	5.4:-59724287	None:intergenic
GCAAACCATGAACAACATGT+GGG	0.683754	5.4:+59723936	None:intergenic
GAAGGTGGTTATCATTGACA+CGG	0.700974	5.4:-59723124	MS.gene26306:CDS
ATTGCATGATTGGAATGACG+AGG	0.708926	5.4:-59723202	MS.gene26306:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTAATTTGATTTTTTTTTCT+TGG	-	chr5.4:59723812-59723831	MS.gene26306:intron	10.0%
!!	ACAAACATATATATTCTTAA+TGG	+	chr5.4:59723466-59723485	None:intergenic	15.0%
!!	ATATATATAATTGTTCTCAT+GGG	+	chr5.4:59723691-59723710	None:intergenic	15.0%
!!	ATTAAGAATATATATGTTTG+TGG	-	chr5.4:59723465-59723484	MS.gene26306:intron	15.0%
!!	TATATATATAATTGTTCTCA+TGG	+	chr5.4:59723692-59723711	None:intergenic	15.0%
!!	TTAAGAATATATATGTTTGT+GGG	-	chr5.4:59723466-59723485	MS.gene26306:intron	15.0%
!!!	AAAATTAGTTTACACATTTT+AGG	-	chr5.4:59723788-59723807	MS.gene26306:intron	15.0%
!!!	AAATTAGTTTACACATTTTA+GGG	-	chr5.4:59723789-59723808	MS.gene26306:intron	15.0%
!!	AAAGATATTAAAGAATTGCA+AGG	-	chr5.4:59724029-59724048	MS.gene26306:CDS	20.0%
!!	TATATATAATTGTTCTCATG+GGG	+	chr5.4:59723690-59723709	None:intergenic	20.0%
!!!	CCATATTTTTCTACTAATAA+CGG	-	chr5.4:59723866-59723885	MS.gene26306:CDS	20.0%
!!!	GAACATTAGAGTATTTTTTA+TGG	+	chr5.4:59723428-59723447	None:intergenic	20.0%
!!!	GTAAACTAATTTTTTTACCA+TGG	+	chr5.4:59723781-59723800	None:intergenic	20.0%
!!!	GTTAAATGAGTTGTTAATTT+TGG	+	chr5.4:59723078-59723097	None:intergenic	20.0%
!	ATATATAATTGTTCTCATGG+GGG	+	chr5.4:59723689-59723708	None:intergenic	25.0%
!	ATATATGTTTGTGGGTTTAA+TGG	-	chr5.4:59723474-59723493	MS.gene26306:intron	25.0%
!	CAATATCAAAGAAAGGTAAA+GGG	-	chr5.4:59724055-59724074	MS.gene26306:CDS	25.0%
!	CCGTTATTAGTAGAAAAATA+TGG	+	chr5.4:59723869-59723888	None:intergenic	25.0%
!	CGAGGAAAATATTATCTAAT+TGG	+	chr5.4:59723892-59723911	None:intergenic	25.0%
!	TTTATTATCCATAGAGATAG+AGG	-	chr5.4:59723516-59723535	MS.gene26306:intron	25.0%
!!	AATTTAGGCAAACATAGTTT+TGG	+	chr5.4:59723752-59723771	None:intergenic	25.0%
!!	TACAAGATTACTCCAATTTT+AGG	-	chr5.4:59724243-59724262	MS.gene26306:CDS	25.0%
!!!	TTTTTTCTTGGTACAAAAGA+GGG	-	chr5.4:59723824-59723843	MS.gene26306:intron	25.0%
!!!	TTTTTTTCTTGGTACAAAAG+AGG	-	chr5.4:59723823-59723842	MS.gene26306:intron	25.0%
	AACAAAATTGCATCTGTTGA+CGG	+	chr5.4:59723341-59723360	None:intergenic	30.0%
	AAGGATGCAATATCAAAGAA+AGG	-	chr5.4:59724048-59724067	MS.gene26306:CDS	30.0%
	AGATGAAAGTAGAAGCAAAA+TGG	+	chr5.4:59723599-59723618	None:intergenic	30.0%
	AGTGGAAAAAATAGAAAACG+AGG	+	chr5.4:59723910-59723929	None:intergenic	30.0%
	ATAGACCTTGATCCTAAAAT+TGG	+	chr5.4:59724258-59724277	None:intergenic	30.0%
	CAACTCATTTAACGAATCAA+TGG	-	chr5.4:59723085-59723104	MS.gene26306:CDS	30.0%
	GAAAACTTAAACTATGTTGC+TGG	-	chr5.4:59723296-59723315	MS.gene26306:intron	30.0%
	GAAAAGAGAACAAACTAAAG+TGG	+	chr5.4:59723928-59723947	None:intergenic	30.0%
	GCAATATCAAAGAAAGGTAA+AGG	-	chr5.4:59724054-59724073	MS.gene26306:CDS	30.0%
	TTACCATGGTGACATAATTT+AGG	+	chr5.4:59723767-59723786	None:intergenic	30.0%
!	AACAGATGCAATTTTGTTGA+AGG	-	chr5.4:59723343-59723362	MS.gene26306:intron	30.0%
!	ATTGTTTTCCTCTATCTCTA+TGG	+	chr5.4:59723527-59723546	None:intergenic	30.0%
!	TATGTTTGAGTTACGAAAGA+AGG	+	chr5.4:59723956-59723975	None:intergenic	30.0%
!	TTTTTCTTGGTACAAAAGAG+GGG	-	chr5.4:59723825-59723844	MS.gene26306:intron	30.0%
!!	ATGGATTAAGTTGATTTCCT+TGG	-	chr5.4:59724206-59724225	MS.gene26306:CDS	30.0%
!!!	TTACTCCAATTTTAGGATCA+AGG	-	chr5.4:59724250-59724269	MS.gene26306:CDS	30.0%
	AAGAGAGAAACGAGAAAGAA+TGG	-	chr5.4:59724187-59724206	MS.gene26306:CDS	35.0%
	ATCAAAGAAAGGTAAAGGGA+AGG	-	chr5.4:59724059-59724078	MS.gene26306:CDS	35.0%
	ATGCATTCCCAACATAAGTT+GGG	+	chr5.4:59723657-59723676	None:intergenic	35.0%
	CTTGCTACTAGAAAAGAGTA+GGG	-	chr5.4:59723626-59723645	MS.gene26306:intron	35.0%
	TACAGTCGATATTGCATGAT+TGG	-	chr5.4:59723989-59724008	MS.gene26306:CDS	35.0%
	TCTTGCTACTAGAAAAGAGT+AGG	-	chr5.4:59723625-59723644	MS.gene26306:intron	35.0%
	TTGCCTAAATTATGTCACCA+TGG	-	chr5.4:59723761-59723780	MS.gene26306:intron	35.0%
!	CTTCTATGACATGTTGATGA+TGG	-	chr5.4:59724149-59724168	MS.gene26306:CDS	35.0%
!!	ATTAAGTTGATTTCCTTGGC+TGG	-	chr5.4:59724210-59724229	MS.gene26306:CDS	35.0%
!!	TCGATTAGTTTCCGATTTGT+TGG	-	chr5.4:59723118-59723137	MS.gene26306:CDS	35.0%
	AAAGAAAGGTAAAGGGAAGG+TGG	-	chr5.4:59724062-59724081	MS.gene26306:CDS	40.0%
	AACACAATGCACTCCATTTG+TGG	+	chr5.4:59723239-59723258	None:intergenic	40.0%
	ACACAATGCACTCCATTTGT+GGG	+	chr5.4:59723238-59723257	None:intergenic	40.0%
	ATTGCATGATTGGAATGACG+AGG	-	chr5.4:59723999-59724018	MS.gene26306:CDS	40.0%
	CAACCAATGACTCAACTTCA+TGG	+	chr5.4:59723166-59723185	None:intergenic	40.0%
	CATACCATTGTACCACTCAT+TGG	-	chr5.4:59723563-59723582	MS.gene26306:intron	40.0%
	CATGCATTCCCAACATAAGT+TGG	+	chr5.4:59723658-59723677	None:intergenic	40.0%
	CTCCATTTGTGGGAATGATT+TGG	+	chr5.4:59723228-59723247	None:intergenic	40.0%
	GAAGGTGGTTATCATTGACA+CGG	-	chr5.4:59724077-59724096	MS.gene26306:CDS	40.0%
	GAGTGGTACAATGGTATGAT+TGG	+	chr5.4:59723561-59723580	None:intergenic	40.0%
	GCAAACCATGAACAACATGT+GGG	+	chr5.4:59723268-59723287	None:intergenic	40.0%
	GCACTTCTCAACCAACAAAT+CGG	+	chr5.4:59723132-59723151	None:intergenic	40.0%
	GTCCAGATATGATAGATCCA+TGG	-	chr5.4:59722961-59722980	MS.gene26306:CDS	40.0%
	GTTCCATGAAGTTGAGTCAT+TGG	-	chr5.4:59723160-59723179	MS.gene26306:CDS	40.0%
	TGCAAACCATGAACAACATG+TGG	+	chr5.4:59723269-59723288	None:intergenic	40.0%
	TGCCAAATCATTCCCACAAA+TGG	-	chr5.4:59723223-59723242	MS.gene26306:intron	40.0%
	TGCCATGGATCTATCATATC+TGG	+	chr5.4:59722966-59722985	None:intergenic	40.0%
!	ACGGTGTTAGACAAAGAGAA+TGG	-	chr5.4:59724096-59724115	MS.gene26306:CDS	40.0%
!	CATGTTGTTCATGGTTTGCA+AGG	-	chr5.4:59723269-59723288	MS.gene26306:intron	40.0%
!	GTTGAGTCATTGGTTGATGT+TGG	-	chr5.4:59723170-59723189	MS.gene26306:CDS	40.0%
!	TTGTTGGTTGAGAAGTGCAA+GGG	-	chr5.4:59723134-59723153	MS.gene26306:CDS	40.0%
!	TTTGGCAAGAGTTTTTGCCA+TGG	+	chr5.4:59723210-59723229	None:intergenic	40.0%
!	TTTGTTGGTTGAGAAGTGCA+AGG	-	chr5.4:59723133-59723152	MS.gene26306:CDS	40.0%
!!	TTGATGATGGTAGTATTCGC+AGG	-	chr5.4:59724162-59724181	MS.gene26306:CDS	40.0%
!!!	AGCACATGAGATGTCGTTTT+GGG	-	chr5.4:59723034-59723053	MS.gene26306:CDS	40.0%
	ACCATTGTACCACTCATTGG+AGG	-	chr5.4:59723566-59723585	MS.gene26306:intron	45.0%
	CAAGCAGAGAATTGATGCCA+TGG	+	chr5.4:59722981-59723000	None:intergenic	45.0%
	CATGGCATCAATTCTCTGCT+TGG	-	chr5.4:59722979-59722998	MS.gene26306:CDS	45.0%
	GATCTCCCACATGTTGTTCA+TGG	-	chr5.4:59723260-59723279	MS.gene26306:intron	45.0%
	GGCACATCCCAACTTATGTT+GGG	-	chr5.4:59723647-59723666	MS.gene26306:intron	45.0%
	GTAGTCACTAAAACCAGCCA+AGG	+	chr5.4:59724226-59724245	None:intergenic	45.0%
	TCCTCCAATGAGTGGTACAA+TGG	+	chr5.4:59723570-59723589	None:intergenic	45.0%
!	GAGTCATTGGTTGATGTTGG+AGG	-	chr5.4:59723173-59723192	MS.gene26306:CDS	45.0%
!	TCATTGGTTGATGTTGGAGG+AGG	-	chr5.4:59723176-59723195	MS.gene26306:CDS	45.0%
!!	CAGCACATGAGATGTCGTTT+TGG	-	chr5.4:59723033-59723052	MS.gene26306:CDS	45.0%
	GGGCACATCCCAACTTATGT+TGG	-	chr5.4:59723646-59723665	MS.gene26306:intron	50.0%
	GGTCTTTGTCCTCCAATGAG+TGG	+	chr5.4:59723578-59723597	None:intergenic	50.0%
!	GGTTGATGTTGGAGGAGGTA+CGG	-	chr5.4:59723181-59723200	MS.gene26306:CDS	50.0%
!	GTTGATGTTGGAGGAGGTAC+GGG	-	chr5.4:59723182-59723201	MS.gene26306:CDS	50.0%
!	TTGATGTTGGAGGAGGTACG+GGG	-	chr5.4:59723183-59723202	MS.gene26306:CDS	50.0%
	CTGAGTATCCCGTGCACATG+CGG	+	chr5.4:59722942-59722961	None:intergenic	55.0%
	AGTGTGGCACCGCATGTGCA+CGG	-	chr5.4:59722930-59722949	MS.gene26306:CDS	60.0%
	GTGTGGCACCGCATGTGCAC+GGG	-	chr5.4:59722931-59722950	MS.gene26306:CDS	65.0%
!	TGGAGGAGGTACGGGGACCA+TGG	-	chr5.4:59723190-59723209	MS.gene26306:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	59722927	59724296	59722927	ID=MS.gene26306
chr5.4	mRNA	59722927	59724296	59722927	ID=MS.gene26306.t1;Parent=MS.gene26306
chr5.4	exon	59723859	59724296	59723859	ID=MS.gene26306.t1.exon1;Parent=MS.gene26306.t1
chr5.4	CDS	59723859	59724296	59723859	ID=cds.MS.gene26306.t1;Parent=MS.gene26306.t1
chr5.4	exon	59722927	59723229	59722927	ID=MS.gene26306.t1.exon2;Parent=MS.gene26306.t1
chr5.4	CDS	59722927	59723229	59722927	ID=cds.MS.gene26306.t1;Parent=MS.gene26306.t1

Gene Sequence

>MS.gene26306

ATGAGTGTGGCACCGCATGTGCACGGGATACTCAGTCCAGATATGATAGATCCATGGCATCAATTCTCTGCTTGGTTAAAAAATGATGATATTACAGCATTTGAAACAGCACATGAGATGTCGTTTTGGGATTATCTTGCACGTGACTCCAAAATTAACAACTCATTTAACGAATCAATGGCAAAAGATACTCGATTAGTTTCCGATTTGTTGGTTGAGAAGTGCAAGGGAGTGTTCCATGAAGTTGAGTCATTGGTTGATGTTGGAGGAGGTACGGGGACCATGGCAAAAACTCTTGCCAAATCATTCCCACAAATGGAGTGCATTGTGTTTGATCTCCCACATGTTGTTCATGGTTTGCAAGGAATTGAAAACTTAAACTATGTTGCTGGAGACATGTTTAAAGAAATTCCGTCAACAGATGCAATTTTGTTGAAGGTAAAATGTTATAATATGCATTTATATATATTACTTGTAATTTTATTGTTTAGAGAAACTCCATAAAAAATACTCTAATGTTCAAAAAACTTGTACTACCATTAAGAATATATATGTTTGTGGGTTTAATGGAAATCTAAAATGATATTGATTTATTATCCATAGAGATAGAGGAAAACAATCAACTAATAGTCCAATCATACCATTGTACCACTCATTGGAGGACAAAGACCATTTTGCTTCTACTTTCATCTGCTAGCTCTTGCTACTAGAAAAGAGTAGGGCACATCCCAACTTATGTTGGGAATGCATGAAGTTACACCCCCATGAGAACAATTATATATATATTTTATCTCAATTTCAACATAGATTATGTTTCTTGAACCAAAACTATGTTTGCCTAAATTATGTCACCATGGTAAAAAAATTAGTTTACACATTTTAGGGTTAATTTGATTTTTTTTTCTTGGTACAAAAGAGGGGATAATTTGTTTTTGTTCACCATATTTTTCTACTAATAACGGCCAATTAGATAATATTTTCCTCGTTTTCTATTTTTTCCACTTTAGTTTGTTCTCTTTTCTCTATCCTTCTTTCGTAACTCAAACATAGTCTTGATGAAATTACAGTCGATATTGCATGATTGGAATGACGAGGAATGTGTAAAGATATTAAAGAATTGCAAGGATGCAATATCAAAGAAAGGTAAAGGGAAGGTGGTTATCATTGACACGGTGTTAGACAAAGAGAATGGAAACATTAATGAATCAGTTGAAACACAACTCTTCTATGACATGTTGATGATGGTAGTATTCGCAGGAAAAGAGAGAAACGAGAAAGAATGGATTAAGTTGATTTCCTTGGCTGGTTTTAGTGACTACAAGATTACTCCAATTTTAGGATCAAGGTCTATGATTGAGATCTATCCATGA

Protein sequence

>MS.gene26306.t1

MSVAPHVHGILSPDMIDPWHQFSAWLKNDDITAFETAHEMSFWDYLARDSKINNSFNESMAKDTRLVSDLLVEKCKGVFHEVESLVDVGGGTGTMAKTLAKSFPQMECIVFDLPHVVHGLQGIENLNYVAGDMFKEIPSTDAILLKSILHDWNDEECVKILKNCKDAISKKGKGKVVIIDTVLDKENGNINESVETQLFYDMLMMVVFAGKERNEKEWIKLISLAGFSDYKITPILGSRSMIEIYP