AlfalfaGEDB | MS.gene26381

M. sativa cultivar XinJiangDaYe / MS.gene26381

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26381.t1	XP_013456959.1	86.4	361	45	3	1	360	1	358	7.10E-77	297.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26381.t1	Q01197	41.7	120	58	5	8	118	10	126	4.6e-08	60.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26381.t1	I3T3J6	86.4	361	45	3	1	360	1	358	5.1e-77	297.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049227	MS.gene26381	0.820935	5.27E-53	-1.69E-46
MS.gene049302	MS.gene26381	0.821894	3.17E-53	-1.69E-46
MS.gene049418	MS.gene26381	0.891373	4.22E-74	-1.69E-46
MS.gene049698	MS.gene26381	0.835275	1.84E-56	-1.69E-46
MS.gene049989	MS.gene26381	0.812701	3.71E-51	-1.69E-46
MS.gene05017	MS.gene26381	0.836005	1.21E-56	-1.69E-46
MS.gene050297	MS.gene26381	0.82318	1.59E-53	-1.69E-46
MS.gene050310	MS.gene26381	0.815652	8.29E-52	-1.69E-46
MS.gene050589	MS.gene26381	0.808013	3.82E-50	-1.69E-46
MS.gene051007	MS.gene26381	0.825942	3.56E-54	-1.69E-46
MS.gene051021	MS.gene26381	0.812555	4.00E-51	-1.69E-46
MS.gene051067	MS.gene26381	0.842844	2.02E-58	-1.69E-46
MS.gene051478	MS.gene26381	0.824661	7.15E-54	-1.69E-46
MS.gene051648	MS.gene26381	0.813694	2.25E-51	-1.69E-46
MS.gene053144	MS.gene26381	0.841389	4.89E-58	-1.69E-46
MS.gene053536	MS.gene26381	0.860055	2.70E-63	-1.69E-46
MS.gene053538	MS.gene26381	0.85535	6.69E-62	-1.69E-46
MS.gene053945	MS.gene26381	0.80395	2.73E-49	-1.69E-46
MS.gene054584	MS.gene26381	0.84961	2.90E-60	-1.69E-46
MS.gene055537	MS.gene26381	0.82741	1.59E-54	-1.69E-46
MS.gene055777	MS.gene26381	0.874816	5.02E-68	-1.69E-46
MS.gene056169	MS.gene26381	0.833044	6.69E-56	-1.69E-46
MS.gene056228	MS.gene26381	0.830188	3.38E-55	-1.69E-46
MS.gene056704	MS.gene26381	0.826037	3.38E-54	-1.69E-46
MS.gene056870	MS.gene26381	0.808944	2.41E-50	-1.69E-46
MS.gene057082	MS.gene26381	0.819989	8.69E-53	-1.69E-46
MS.gene05709	MS.gene26381	0.83066	2.59E-55	-1.69E-46
MS.gene058015	MS.gene26381	0.801616	8.28E-49	-1.69E-46
MS.gene058196	MS.gene26381	0.816206	6.23E-52	-1.69E-46
MS.gene058197	MS.gene26381	0.853178	2.84E-61	-1.69E-46
MS.gene058198	MS.gene26381	0.851399	9.11E-61	-1.69E-46
MS.gene058823	MS.gene26381	0.824146	9.45E-54	-1.69E-46
MS.gene05894	MS.gene26381	0.81465	1.38E-51	-1.69E-46
MS.gene059300	MS.gene26381	0.864846	9.06E-65	-1.69E-46
MS.gene059424	MS.gene26381	0.861113	1.29E-63	-1.69E-46
MS.gene060492	MS.gene26381	0.80317	3.96E-49	-1.69E-46
MS.gene06057	MS.gene26381	0.854327	1.33E-61	-1.69E-46
MS.gene060962	MS.gene26381	0.812953	3.27E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene26381.t1	MTR_4g088385	84.573	363	48	6	1	360	1	358	1.01e-141	406
MS.gene26381.t1	MTR_2g014400	52.381	147	56	5	221	359	120	260	1.83e-17	81.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTGTTCTGGTTTGTTTAA+TGG	0.166365	4.1:+21911289	None:intergenic
GACTGTCTCTGGCATGAATT+TGG	0.206853	4.1:+21911386	None:intergenic
TTCGAGTTATATCCTTCTTC+TGG	0.209481	4.1:+21910950	None:intergenic
ATTCGGGTACTTGTTGTTAT+AGG	0.218132	4.1:+21911078	None:intergenic
CTTCAAACTGCTCTTGATTC+TGG	0.250818	4.1:+21910411	None:intergenic
TTTAATGGTGTTTCATTGTT+AGG	0.261763	4.1:+21911304	None:intergenic
TGTTGTTGTTGTTATTGTTC+TGG	0.280406	4.1:+21910813	None:intergenic
ATGAAAACTGGTTGTTGTTC+TGG	0.303072	4.1:+21911277	None:intergenic
CAAGTATTCTTCTAGTTCTA+AGG	0.327940	4.1:-21910993	MS.gene26381:CDS
GAAAACAGTTATGGTTTATA+TGG	0.334786	4.1:-21911244	MS.gene26381:CDS
GGTTTATATGGTCATGATAC+TGG	0.336006	4.1:-21911232	MS.gene26381:CDS
ATTGAGTTGTAACCTTCTTC+TGG	0.340437	4.1:+21910746	None:intergenic
TTCATATGATTGGAAAGTGT+TGG	0.393181	4.1:+21911168	None:intergenic
TCGGTTTCCGGGATGAAAAC+TGG	0.395977	4.1:+21911265	None:intergenic
GCTGAAGAATTGACTGTCTC+TGG	0.398942	4.1:+21911375	None:intergenic
TTGGTGTCACTAACTTCATT+CGG	0.404949	4.1:+21911061	None:intergenic
TGTTGTTATAGGAATCTTGC+TGG	0.405186	4.1:+21911089	None:intergenic
TCATATGATTGGAAAGTGTT+GGG	0.422874	4.1:+21911169	None:intergenic
GTTGTCGTAGTTGTTGTTGT+TGG	0.424284	4.1:+21911018	None:intergenic
CCGGAAACCGAAAACAGTTA+TGG	0.426235	4.1:-21911253	MS.gene26381:CDS
TCGAGTTATATCCTTCTTCT+GGG	0.432731	4.1:+21910951	None:intergenic
TGGTGTCACTAACTTCATTC+GGG	0.435111	4.1:+21911062	None:intergenic
TTGAGTTGTAACCTTCTTCT+GGG	0.440468	4.1:+21910747	None:intergenic
GTAAGATGTTCCCGTTAGTT+TGG	0.449964	4.1:+21911042	None:intergenic
AACGCTAAGTTTACTGAAGA+AGG	0.456600	4.1:-21910854	MS.gene26381:CDS
TTCAATGGAGATTCATCTAA+AGG	0.457617	4.1:-21910530	MS.gene26381:CDS
AATGAGTGACACAAGAGTTA+TGG	0.465302	4.1:-21910606	MS.gene26381:CDS
ATGAAAGGGTTGTTAAGAAT+AGG	0.475071	4.1:+21911416	None:intergenic
AAATACAATCCAACATTCAA+TGG	0.477159	4.1:-21910545	MS.gene26381:CDS
TTGTTTGTGTTAGTAGTACT+AGG	0.484396	4.1:+21911199	None:intergenic
GTTAGTGACACCAAACTAAC+GGG	0.485500	4.1:-21911052	MS.gene26381:CDS
GAGTTGTGAACTCTGAGAAT+TGG	0.491359	4.1:-21910508	MS.gene26381:CDS
GATGAATCTCCATTGAATGT+TGG	0.492045	4.1:+21910536	None:intergenic
TTGTTAAGAATAGGAAAGAA+AGG	0.493863	4.1:+21911425	None:intergenic
TTGTGTTAGTAGTACTAGGA+GGG	0.504780	4.1:+21911203	None:intergenic
AACACAAAGTTCCCAGAAGA+AGG	0.504928	4.1:-21910758	MS.gene26381:CDS
GAATTTGGAGAGAAAATGAA+AGG	0.529100	4.1:+21911401	None:intergenic
AATAACAAGAACTCTATGAA+TGG	0.533344	4.1:-21910437	MS.gene26381:CDS
TACAACAATGGTGAGAGGCA+AGG	0.586180	4.1:-21910629	MS.gene26381:CDS
TGAAGAAGGATACAAATCCA+TGG	0.589242	4.1:-21910840	MS.gene26381:CDS
TTTGTGTTAGTAGTACTAGG+AGG	0.589979	4.1:+21911202	None:intergenic
AATTTGGAGAGAAAATGAAA+GGG	0.593601	4.1:+21911402	None:intergenic
AGTTAGTGACACCAAACTAA+CGG	0.603223	4.1:-21911053	MS.gene26381:CDS
AGTAACAACAATTACAACAA+TGG	0.621284	4.1:-21910641	MS.gene26381:CDS
GACACAAGAGTTATGGAAGG+TGG	0.628338	4.1:-21910599	MS.gene26381:CDS
ACAATTACAACAATGGTGAG+AGG	0.652438	4.1:-21910634	MS.gene26381:CDS
AGTGACACAAGAGTTATGGA+AGG	0.692016	4.1:-21910602	MS.gene26381:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATATAAACCATAACTGTTTT+CGG	+	chr4.1:21910587-21910606	None:intergenic	20.0%
!	AAATACAATCCAACATTCAA+TGG	-	chr4.1:21911285-21911304	MS.gene26381:CDS	25.0%
!	AATAACAAGAACTCTATGAA+TGG	-	chr4.1:21911393-21911412	MS.gene26381:CDS	25.0%
!	AATTTGGAGAGAAAATGAAA+GGG	+	chr4.1:21910431-21910450	None:intergenic	25.0%
!	AGTAACAACAATTACAACAA+TGG	-	chr4.1:21911189-21911208	MS.gene26381:CDS	25.0%
!	GAAAACAGTTATGGTTTATA+TGG	-	chr4.1:21910586-21910605	MS.gene26381:CDS	25.0%
!	TACAACAAAAAAGGTTACTT+TGG	-	chr4.1:21911345-21911364	MS.gene26381:CDS	25.0%
!	TTGTTAAGAATAGGAAAGAA+AGG	+	chr4.1:21910408-21910427	None:intergenic	25.0%
!	TTTAATGGTGTTTCATTGTT+AGG	+	chr4.1:21910529-21910548	None:intergenic	25.0%
!!	TTTTTATGATGTTAACTCTG+AGG	-	chr4.1:21911260-21911279	MS.gene26381:CDS	25.0%
!!!	AAAGAAAGGAATTTTGAGAT+GGG	+	chr4.1:21910394-21910413	None:intergenic	25.0%
!!!	ACGAAGTTTTTTCATATGAT+TGG	+	chr4.1:21910675-21910694	None:intergenic	25.0%
	ATGAAAGGGTTGTTAAGAAT+AGG	+	chr4.1:21910417-21910436	None:intergenic	30.0%
	GAATTTGGAGAGAAAATGAA+AGG	+	chr4.1:21910432-21910451	None:intergenic	30.0%
	GAGAATTGGTACAACAAAAA+AGG	-	chr4.1:21911336-21911355	MS.gene26381:CDS	30.0%
	TTCAATGGAGATTCATCTAA+AGG	-	chr4.1:21911300-21911319	MS.gene26381:CDS	30.0%
	TTGTTTGTGTTAGTAGTACT+AGG	+	chr4.1:21910634-21910653	None:intergenic	30.0%
	TTTCTACAACAACAACAACA+AGG	-	chr4.1:21910942-21910961	MS.gene26381:CDS	30.0%
!	TGTTGTTGTTGTTATTGTTC+TGG	+	chr4.1:21911020-21911039	None:intergenic	30.0%
!	TTCATATGATTGGAAAGTGT+TGG	+	chr4.1:21910665-21910684	None:intergenic	30.0%
!!	CAAGTATTCTTCTAGTTCTA+AGG	-	chr4.1:21910837-21910856	MS.gene26381:CDS	30.0%
!!	GAAAGAAAGGAATTTTGAGA+TGG	+	chr4.1:21910395-21910414	None:intergenic	30.0%
!!	TCATATGATTGGAAAGTGTT+GGG	+	chr4.1:21910664-21910683	None:intergenic	30.0%
!!	TGTTGTTCTGGTTTGTTTAA+TGG	+	chr4.1:21910544-21910563	None:intergenic	30.0%
	AACAAAAAGTTCCCAGAAGA+AGG	-	chr4.1:21910868-21910887	MS.gene26381:CDS	35.0%
	AACGCTAAGTTTACTGAAGA+AGG	-	chr4.1:21910976-21910995	MS.gene26381:CDS	35.0%
	AATGAGTGACACAAGAGTTA+TGG	-	chr4.1:21911224-21911243	MS.gene26381:CDS	35.0%
	ACAATTACAACAATGGTGAG+AGG	-	chr4.1:21911196-21911215	MS.gene26381:CDS	35.0%
	AGTTAGTGACACCAAACTAA+CGG	-	chr4.1:21910777-21910796	MS.gene26381:CDS	35.0%
	GATGAATCTCCATTGAATGT+TGG	+	chr4.1:21911297-21911316	None:intergenic	35.0%
	GGTTTATATGGTCATGATAC+TGG	-	chr4.1:21910598-21910617	MS.gene26381:CDS	35.0%
	TCGAGTTATATCCTTCTTCT+GGG	+	chr4.1:21910882-21910901	None:intergenic	35.0%
	TGAAGAAGGATACAAATCCA+TGG	-	chr4.1:21910990-21911009	MS.gene26381:CDS	35.0%
	TGTTGTTATAGGAATCTTGC+TGG	+	chr4.1:21910744-21910763	None:intergenic	35.0%
	TTGTGTTAGTAGTACTAGGA+GGG	+	chr4.1:21910630-21910649	None:intergenic	35.0%
	TTTGTGTTAGTAGTACTAGG+AGG	+	chr4.1:21910631-21910650	None:intergenic	35.0%
!	ATGAAAACTGGTTGTTGTTC+TGG	+	chr4.1:21910556-21910575	None:intergenic	35.0%
!	ATTGAGTTGTAACCTTCTTC+TGG	+	chr4.1:21911087-21911106	None:intergenic	35.0%
!	GTTGTTGTTGTTGTTGACAT+GGG	+	chr4.1:21910488-21910507	None:intergenic	35.0%
!	TGTTGTTGTTGTTGTTGACA+TGG	+	chr4.1:21910489-21910508	None:intergenic	35.0%
!	TTCGAGTTATATCCTTCTTC+TGG	+	chr4.1:21910883-21910902	None:intergenic	35.0%
!	TTGAGTTGTAACCTTCTTCT+GGG	+	chr4.1:21911086-21911105	None:intergenic	35.0%
!!	ATTCGGGTACTTGTTGTTAT+AGG	+	chr4.1:21910755-21910774	None:intergenic	35.0%
!!	TTGGTGTCACTAACTTCATT+CGG	+	chr4.1:21910772-21910791	None:intergenic	35.0%
!!!	GTTATTGTTCTGGTTTTCCA+TGG	+	chr4.1:21911010-21911029	None:intergenic	35.0%
	AACACAAAGTTCCCAGAAGA+AGG	-	chr4.1:21911072-21911091	MS.gene26381:CDS	40.0%
	AGTGACACAAGAGTTATGGA+AGG	-	chr4.1:21911228-21911247	MS.gene26381:CDS	40.0%
	CTTCAAACTGCTCTTGATTC+TGG	+	chr4.1:21911422-21911441	None:intergenic	40.0%
	GTAAGATGTTCCCGTTAGTT+TGG	+	chr4.1:21910791-21910810	None:intergenic	40.0%
	GTTAGTGACACCAAACTAAC+GGG	-	chr4.1:21910778-21910797	MS.gene26381:CDS	40.0%
!	ACAACAACCAGTTTTCATCC+CGG	-	chr4.1:21910558-21910577	MS.gene26381:CDS	40.0%
!	CATAACTGTTTTCGGTTTCC+GGG	+	chr4.1:21910579-21910598	None:intergenic	40.0%
!	CCATAACTGTTTTCGGTTTC+CGG	+	chr4.1:21910580-21910599	None:intergenic	40.0%
!	CGTAGTTGTTGTTGTTGTTC+TGG	+	chr4.1:21911116-21911135	None:intergenic	40.0%
!	GAGTTGTGAACTCTGAGAAT+TGG	-	chr4.1:21911322-21911341	MS.gene26381:CDS	40.0%
!	GTTGTCGTAGTTGTTGTTGT+TGG	+	chr4.1:21910815-21910834	None:intergenic	40.0%
!!	TGGTGTCACTAACTTCATTC+GGG	+	chr4.1:21910771-21910790	None:intergenic	40.0%
	CCGGAAACCGAAAACAGTTA+TGG	-	chr4.1:21910577-21910596	MS.gene26381:CDS	45.0%
	GACACAAGAGTTATGGAAGG+TGG	-	chr4.1:21911231-21911250	MS.gene26381:CDS	45.0%
	GACTGTCTCTGGCATGAATT+TGG	+	chr4.1:21910447-21910466	None:intergenic	45.0%
	GCTGAAGAATTGACTGTCTC+TGG	+	chr4.1:21910458-21910477	None:intergenic	45.0%
	TACAACAATGGTGAGAGGCA+AGG	-	chr4.1:21911201-21911220	MS.gene26381:CDS	45.0%
	TCGGTTTCCGGGATGAAAAC+TGG	+	chr4.1:21910568-21910587	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	21910385	21911467	21910385	ID=MS.gene26381
chr4.1	mRNA	21910385	21911467	21910385	ID=MS.gene26381.t1;Parent=MS.gene26381
chr4.1	exon	21910385	21911467	21910385	ID=MS.gene26381.t1.exon1;Parent=MS.gene26381.t1
chr4.1	CDS	21910385	21911467	21910385	ID=cds.MS.gene26381.t1;Parent=MS.gene26381.t1

Gene Sequence

>MS.gene26381

ATGGCTCCCATCTCAAAATTCCTTTCTTTCCTATTCTTAACAACCCTTTCATTTTCTCTCCAAATTCATGCCAGAGACAGTCAATTCTTCAGCAAAGTCACCCATGTCAACAACAACAACAACAACAATGTTAGAGAAATTCCTAACAATGAAACACCATTAAACAAACCAGAACAACAACCAGTTTTCATCCCGGAAACCGAAAACAGTTATGGTTTATATGGTCATGATACTGGTCTACACCCTCCTAGTACTACTAACACAAACAATGTTGATCCCAACACTTTCCAATCATATGAAAAAACTTCGTCACACGATGAAATCAACGACAATAAAGTTAACAATTACTTCAACTACCAGCAAGATTCCTATAACAACAAGTACCCGAATGAAGTTAGTGACACCAAACTAACGGGAACATCTTACACCAACAACAACAACTACGACAACAACAAGTATTCTTCTAGTTCTAAGGACTTTAGCAACAAAAAGTTCCCAGAAGAAGGATATAACTCGAATGAAAATCAGAACAACAACAACAACAACGATAACAAGTATTTCTACAACAACAACAACAAGGATTCTTCTAGCAACGCTAAGTTTACTGAAGAAGGATACAAATCCATGGAAAACCAGAACAATAACAACAACAACAAGTATTTCTACAACAACAAAGATGCTTCTAGCAACACAAAGTTCCCAGAAGAAGGTTACAACTCAATGCAAAACCAGAACAACAACAACAACTACGAGAAGTATAACTACAACAACAAAGTTGCTGTTAATGACAAATACAGCTTCAAAAGTAACAACAATTACAACAATGGTGAGAGGCAAGGAATGAGTGACACAAGAGTTATGGAAGGTGGAAAATATTTTTATGATGTTAACTCTGAGGAGAAATACAATCCAACATTCAATGGAGATTCATCTAAAGGAGTTGTGAACTCTGAGAATTGGTACAACAAAAAAGGTTACTTTGGTAACAACAATGTGAACAATTATGAGAATAACAAGAACTCTATGAATGGTTACCAGAATCAAGAGCAGTTTGAAGATGATCAAGAAGAATTTGAGCCTTGA

Protein sequence

>MS.gene26381.t1

MAPISKFLSFLFLTTLSFSLQIHARDSQFFSKVTHVNNNNNNNVREIPNNETPLNKPEQQPVFIPETENSYGLYGHDTGLHPPSTTNTNNVDPNTFQSYEKTSSHDEINDNKVNNYFNYQQDSYNNKYPNEVSDTKLTGTSYTNNNNYDNNKYSSSSKDFSNKKFPEEGYNSNENQNNNNNNDNKYFYNNNNKDSSSNAKFTEEGYKSMENQNNNNNNKYFYNNKDASSNTKFPEEGYNSMQNQNNNNNYEKYNYNNKVAVNDKYSFKSNNNYNNGERQGMSDTRVMEGGKYFYDVNSEEKYNPTFNGDSSKGVVNSENWYNKKGYFGNNNVNNYENNKNSMNGYQNQEQFEDDQEEFEP