AlfalfaGEDB | MS.gene055777

M. sativa cultivar XinJiangDaYe / MS.gene055777

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055777.t1	XP_013465149.1	73	281	25	4	1	268	1	243	5.10E-72	281.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055777.t1	A0A072VAX8	73.0	281	25	4	1	268	1	243	3.7e-72	281.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene055777	MS.gene059300	0.800131	1.66E-48	-1.69E-46
MS.gene055777	MS.gene063170	0.807008	6.24E-50	-1.69E-46
MS.gene055777	MS.gene065643	0.825233	5.24E-54	-1.69E-46
MS.gene055777	MS.gene068190	0.814771	1.30E-51	-1.69E-46
MS.gene055777	MS.gene07246	0.8238	1.14E-53	-1.69E-46
MS.gene055777	MS.gene073606	0.804604	1.99E-49	-1.69E-46
MS.gene055777	MS.gene08048	0.862886	3.69E-64	-1.69E-46
MS.gene055777	MS.gene08366	0.816483	5.40E-52	-1.69E-46
MS.gene055777	MS.gene20768	0.825766	3.92E-54	-1.69E-46
MS.gene055777	MS.gene24982	0.809533	1.80E-50	-1.69E-46
MS.gene055777	MS.gene26381	0.874816	5.02E-68	-1.69E-46
MS.gene055777	MS.gene26665	0.801531	8.62E-49	-1.69E-46
MS.gene055777	MS.gene29731	0.871003	9.51E-67	-1.69E-46
MS.gene055777	MS.gene32024	0.827341	1.65E-54	-1.69E-46
MS.gene055777	MS.gene42296	0.812318	4.50E-51	-1.69E-46
MS.gene055777	MS.gene53003	0.811401	7.13E-51	-1.69E-46
MS.gene055777	MS.gene53004	0.806292	8.83E-50	-1.69E-46
MS.gene055777	MS.gene55782	0.801376	9.27E-49	-1.69E-46
MS.gene055777	MS.gene62603	0.831026	2.11E-55	-1.69E-46
MS.gene055777	MS.gene70838	0.801433	9.02E-49	-1.69E-46
MS.gene055777	MS.gene71461	0.805216	1.49E-49	-1.69E-46
MS.gene055777	MS.gene77669	0.82116	4.68E-53	-1.69E-46
MS.gene055777	MS.gene84497	0.816076	6.66E-52	-1.69E-46
MS.gene055777	MS.gene90732	0.802087	6.62E-49	-1.69E-46
MS.gene055777	MS.gene94168	0.815856	7.46E-52	-1.69E-46
MS.gene055777	MS.gene99526	0.808728	2.69E-50	-1.69E-46
MS.gene055777	MS.gene99878	0.803425	3.51E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055777.t1	MTR_2g090660	87.687	268	8	3	1	268	1	243	3.48e-162	449

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene055777.t1	AT1G03820	31.752	274	129	9	1	268	1	222	2.54e-15	73.6

Find 42 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAGAAGTATAAGAACGTTAA+TGG	0.240828	2.1:+10266039	MS.gene055777:CDS
TTTCCTTTGTTGGAGAATAT+TGG	0.297541	2.1:-10265702	None:intergenic
ATAGAGGCCGTAACCATTTC+CGG	0.316661	2.1:-10265665	None:intergenic
AACGTTAATGGATATGAATC+AGG	0.319643	2.1:+10266051	MS.gene055777:CDS
AATGAGTGACACAAGAATTC+TGG	0.325157	2.1:+10265978	MS.gene055777:CDS
TCAAAATGAGTTTGAGACTA+TGG	0.340281	2.1:+10266119	MS.gene055777:CDS
GGAAATGGTTACGGCCTCTA+TGG	0.371284	2.1:+10265667	MS.gene055777:CDS
GCCAGTGCTGGTGCCGGTGC+TGG	0.371669	2.1:-10265535	None:intergenic
ATATTAGTTGATTCAATCTC+TGG	0.384653	2.1:-10265601	None:intergenic
TCAATCTCTGGTGCAGCTGC+AGG	0.412954	2.1:-10265589	None:intergenic
GTTGAAACCGGAAATGGTTA+CGG	0.443604	2.1:+10265658	MS.gene055777:CDS
GCAGGTTCTTGTGCCAGTGC+TGG	0.462107	2.1:-10265547	None:intergenic
TTGAGGGTCTTCAACATTGT+GGG	0.464139	2.1:-10265500	None:intergenic
TCTTGTGCCGGAGCTGATGC+AGG	0.485604	2.1:-10265565	None:intergenic
GACACAAGAATTCTGGAGAA+TGG	0.486876	2.1:+10265985	MS.gene055777:CDS
ACAAGAACCTGCATCAGCTC+CGG	0.499228	2.1:+10265558	MS.gene055777:CDS
GGTTCTGGTGCTGGTCCTGA+TGG	0.499252	2.1:-10265625	None:intergenic
AACAATTACAACAGCTACAA+TGG	0.499851	2.1:+10265904	MS.gene055777:CDS
GCAGCTGCAGGTTCTTGTGC+CGG	0.500329	2.1:-10265577	None:intergenic
TCCAGCACCGGCACCAGCAC+TGG	0.501443	2.1:+10265534	MS.gene055777:CDS
CTGGAGATTGAGGTAATTGA+GGG	0.513332	2.1:-10265516	None:intergenic
GCTGGAGATTGAGGTAATTG+AGG	0.526485	2.1:-10265517	None:intergenic
ATCTTCAAAATCTGTTAATG+TGG	0.531207	2.1:-10265734	None:intergenic
GAATCAACTAATATACCATC+AGG	0.531610	2.1:+10265610	MS.gene055777:CDS
TCTTGTGCCAGTGCTGGTGC+CGG	0.536120	2.1:-10265541	None:intergenic
TTGTTGCGGAAGTTTGTTTG+AGG	0.543740	2.1:-10265838	None:intergenic
ATTGAGGGTCTTCAACATTG+TGG	0.549263	2.1:-10265501	None:intergenic
AGGGTCTTCAACATTGTGGG+TGG	0.554411	2.1:-10265497	None:intergenic
TAGCCAATATTCTCCAACAA+AGG	0.556044	2.1:+10265699	MS.gene055777:CDS
AACAACAATTATGAGAGGAA+AGG	0.589017	2.1:+10265949	MS.gene055777:CDS
AATTATGAGAGGAAAGGAGA+AGG	0.592187	2.1:+10265955	MS.gene055777:CDS
AATGGATATGAATCAGGGAG+AGG	0.596788	2.1:+10266057	MS.gene055777:CDS
GGTGCCGGTGCTGGAGATTG+AGG	0.597375	2.1:-10265526	None:intergenic
ATTACCTCAATCTCCAGCAC+CGG	0.607846	2.1:+10265522	MS.gene055777:CDS
AGAGGAAGCACTGAAAATGA+AGG	0.608381	2.1:+10266075	MS.gene055777:CDS
TGTACACTTCATTGTTGTTG+CGG	0.612560	2.1:-10265852	None:intergenic
TGGCTTGAATTTGGGGTGAG+AGG	0.613693	2.1:-10265435	None:intergenic
ACGTTAATGGATATGAATCA+GGG	0.622449	2.1:+10266052	MS.gene055777:CDS
GGCTAGAATCTACACCATAG+AGG	0.623026	2.1:-10265681	None:intergenic
TTACAACAGCTACAATGGAA+AGG	0.631436	2.1:+10265909	MS.gene055777:CDS
ACAATAACAACAATTATGAG+AGG	0.640620	2.1:+10265944	MS.gene055777:CDS
GAGAAGCAGCAAGAAGCACA+AGG	0.650203	2.1:+10266150	MS.gene055777:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATAATAAAAGCTACAAAAA+AGG	+	chr2.1:10265811-10265830	MS.gene055777:CDS	20.0%
!	ACAATAACAACAATTATGAG+AGG	+	chr2.1:10265944-10265963	MS.gene055777:CDS	25.0%
!	ATCTTCAAAATCTGTTAATG+TGG	-	chr2.1:10265737-10265756	None:intergenic	25.0%
!!	AAATCTGTTAATGTGGTTTT+TGG	-	chr2.1:10265730-10265749	None:intergenic	25.0%
!!	ATATTAGTTGATTCAATCTC+TGG	-	chr2.1:10265604-10265623	None:intergenic	25.0%
	AACAACAATTATGAGAGGAA+AGG	+	chr2.1:10265949-10265968	MS.gene055777:CDS	30.0%
	AACAATTACAACAGCTACAA+TGG	+	chr2.1:10265904-10265923	MS.gene055777:CDS	30.0%
	ACAAGAAAAAGAAAGAGACT+TGG	-	chr2.1:10265405-10265424	None:intergenic	30.0%
	GAATCAACTAATATACCATC+AGG	+	chr2.1:10265610-10265629	MS.gene055777:CDS	30.0%
	TCAACTAAAAATTGAGGTTC+TGG	-	chr2.1:10265643-10265662	None:intergenic	30.0%
	TTTCCTTTGTTGGAGAATAT+TGG	-	chr2.1:10265705-10265724	None:intergenic	30.0%
!	AACGTTAATGGATATGAATC+AGG	+	chr2.1:10266051-10266070	MS.gene055777:CDS	30.0%
!	ACGTTAATGGATATGAATCA+GGG	+	chr2.1:10266052-10266071	MS.gene055777:CDS	30.0%
!	AGTCTCTTTCTTTTTCTTGT+TGG	+	chr2.1:10265405-10265424	MS.gene055777:CDS	30.0%
!	GAGAAGTATAAGAACGTTAA+TGG	+	chr2.1:10266039-10266058	MS.gene055777:CDS	30.0%
!	TCAAAATGAGTTTGAGACTA+TGG	+	chr2.1:10266119-10266138	MS.gene055777:CDS	30.0%
!!!	CCTCAATTTTTAGTTGAAAC+CGG	+	chr2.1:10265646-10265665	MS.gene055777:CDS	30.0%
!!!	TTTTTAGTTGAAACCGGAAA+TGG	+	chr2.1:10265652-10265671	MS.gene055777:CDS	30.0%
	AATGAGTGACACAAGAATTC+TGG	+	chr2.1:10265978-10265997	MS.gene055777:CDS	35.0%
	AATTATGAGAGGAAAGGAGA+AGG	+	chr2.1:10265955-10265974	MS.gene055777:CDS	35.0%
	CCGGTTTCAACTAAAAATTG+AGG	-	chr2.1:10265649-10265668	None:intergenic	35.0%
	TAGCCAATATTCTCCAACAA+AGG	+	chr2.1:10265699-10265718	MS.gene055777:CDS	35.0%
	TTACAACAGCTACAATGGAA+AGG	+	chr2.1:10265909-10265928	MS.gene055777:CDS	35.0%
!	TGTACACTTCATTGTTGTTG+CGG	-	chr2.1:10265855-10265874	None:intergenic	35.0%
!!	GTTTTGTCTGGCTTGAATTT+GGG	-	chr2.1:10265446-10265465	None:intergenic	35.0%
!!	TGTTTTGTCTGGCTTGAATT+TGG	-	chr2.1:10265447-10265466	None:intergenic	35.0%
!!	TTTTGTCTGGCTTGAATTTG+GGG	-	chr2.1:10265445-10265464	None:intergenic	35.0%
!!!	GCTAAAGAACTTGTTTTGTC+TGG	-	chr2.1:10265458-10265477	None:intergenic	35.0%
!!!	GTTTTTGGAGTTTCCTTTGT+TGG	-	chr2.1:10265715-10265734	None:intergenic	35.0%
	AATGGATATGAATCAGGGAG+AGG	+	chr2.1:10266057-10266076	MS.gene055777:CDS	40.0%
	ATTGAGGGTCTTCAACATTG+TGG	-	chr2.1:10265504-10265523	None:intergenic	40.0%
	CTGGAGATTGAGGTAATTGA+GGG	-	chr2.1:10265519-10265538	None:intergenic	40.0%
	GTTGAAACCGGAAATGGTTA+CGG	+	chr2.1:10265658-10265677	MS.gene055777:CDS	40.0%
	TTGAGGGTCTTCAACATTGT+GGG	-	chr2.1:10265503-10265522	None:intergenic	40.0%
!	AAAAATTGAGGTTCTGGTGC+TGG	-	chr2.1:10265637-10265656	None:intergenic	40.0%
!	GACACAAGAATTCTGGAGAA+TGG	+	chr2.1:10265985-10266004	MS.gene055777:CDS	40.0%
!	TTGTTGCGGAAGTTTGTTTG+AGG	-	chr2.1:10265841-10265860	None:intergenic	40.0%
!!	AGAGGAAGCACTGAAAATGA+AGG	+	chr2.1:10266075-10266094	MS.gene055777:CDS	40.0%
	ATAGAGGCCGTAACCATTTC+CGG	-	chr2.1:10265668-10265687	None:intergenic	45.0%
	ATTACCTCAATCTCCAGCAC+CGG	+	chr2.1:10265522-10265541	MS.gene055777:CDS	45.0%
	GCTGGAGATTGAGGTAATTG+AGG	-	chr2.1:10265520-10265539	None:intergenic	45.0%
	GGCTAGAATCTACACCATAG+AGG	-	chr2.1:10265684-10265703	None:intergenic	45.0%
	ACAAGAACCTGCATCAGCTC+CGG	+	chr2.1:10265558-10265577	MS.gene055777:CDS	50.0%
	AGGGTCTTCAACATTGTGGG+TGG	-	chr2.1:10265500-10265519	None:intergenic	50.0%
	GGAAATGGTTACGGCCTCTA+TGG	+	chr2.1:10265667-10265686	MS.gene055777:CDS	50.0%
!	GAGAAGCAGCAAGAAGCACA+AGG	+	chr2.1:10266150-10266169	MS.gene055777:CDS	50.0%
!	TGGCTTGAATTTGGGGTGAG+AGG	-	chr2.1:10265438-10265457	None:intergenic	50.0%
	TCAATCTCTGGTGCAGCTGC+AGG	-	chr2.1:10265592-10265611	None:intergenic	55.0%
	GCAGCTGCAGGTTCTTGTGC+CGG	-	chr2.1:10265580-10265599	None:intergenic	60.0%
	GCAGGTTCTTGTGCCAGTGC+TGG	-	chr2.1:10265550-10265569	None:intergenic	60.0%
	TCTTGTGCCGGAGCTGATGC+AGG	-	chr2.1:10265568-10265587	None:intergenic	60.0%
!!	GGTTCTGGTGCTGGTCCTGA+TGG	-	chr2.1:10265628-10265647	None:intergenic	60.0%
!!	TCTTGTGCCAGTGCTGGTGC+CGG	-	chr2.1:10265544-10265563	None:intergenic	60.0%
!!	GGTGCCGGTGCTGGAGATTG+AGG	-	chr2.1:10265529-10265548	None:intergenic	65.0%
!!	TCCAGCACCGGCACCAGCAC+TGG	+	chr2.1:10265534-10265553	MS.gene055777:CDS	70.0%
!!	GCCAGTGCTGGTGCCGGTGC+TGG	-	chr2.1:10265538-10265557	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	10265385	10266191	10265385	ID=MS.gene055777
chr2.1	mRNA	10265385	10266191	10265385	ID=MS.gene055777.t1;Parent=MS.gene055777
chr2.1	exon	10265385	10266191	10265385	ID=MS.gene055777.t1.exon1;Parent=MS.gene055777.t1
chr2.1	CDS	10265385	10266191	10265385	ID=cds.MS.gene055777.t1;Parent=MS.gene055777.t1

Gene Sequence

>MS.gene055777

ATGGCTTCTCTTGCAAACCAAGTCTCTTTCTTTTTCTTGTTGGTACTTTTCCTCTCACCCCAAATTCAAGCCAGACAAAACAAGTTCTTTAGCTTCTTTACTCATTTCAAAACCACCCACAATGTTGAAGACCCTCAATTACCTCAATCTCCAGCACCGGCACCAGCACTGGCACAAGAACCTGCATCAGCTCCGGCACAAGAACCTGCAGCTGCACCAGAGATTGAATCAACTAATATACCATCAGGACCAGCACCAGAACCTCAATTTTTAGTTGAAACCGGAAATGGTTACGGCCTCTATGGTGTAGATTCTAGCCAATATTCTCCAACAAAGGAAACTCCAAAAACCACATTAACAGATTTTGAAGATGAGCTTCTCAATGAAGACTTCAATGATAACAAGCTTCTCAATGAAGATTTCAATGATAATAAAAGCTACAAAAAAGGATATCCTCAAACAAACTTCCGCAACAACAATGAAGTGTACACAAAAAACTACAACAATGAAGAGAACAAGAACAATTACAACAGCTACAATGGAAAGGAGTTTTACAACAACAATAACAACAATTATGAGAGGAAAGGAGAAGGAATGAGTGACACAAGAATTCTGGAGAATGGAAAGTACTATTACAATGTAAATAGTGAAAATGAGAAGTATAAGAACGTTAATGGATATGAATCAGGGAGAGGAAGCACTGAAAATGAAGGTAACTATGAAAAAAATCAGTATCAAAATGAGTTTGAGACTATGGAAGAGTATGAGAAGCAGCAAGAAGCACAAGGTTACACATACACACCATGA

Protein sequence

>MS.gene055777.t1

MASLANQVSFFFLLVLFLSPQIQARQNKFFSFFTHFKTTHNVEDPQLPQSPAPAPALAQEPASAPAQEPAAAPEIESTNIPSGPAPEPQFLVETGNGYGLYGVDSSQYSPTKETPKTTLTDFEDELLNEDFNDNKLLNEDFNDNKSYKKGYPQTNFRNNNEVYTKNYNNEENKNNYNSYNGKEFYNNNNNNYERKGEGMSDTRILENGKYYYNVNSENEKYKNVNGYESGRGSTENEGNYEKNQYQNEFETMEEYEKQQEAQGYTYTP