AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26908


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26908.t1 MTR_1g104630 79.899 199 32 3 1 197 1 193 1.65e-109 311
MS.gene26908.t1 MTR_5g006130 70.833 216 37 3 1 197 1 209 1.18e-102 295
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26908.t1 AT1G03130 75.145 173 30 2 38 197 32 204 2.77e-88 258
MS.gene26908.t1 AT4G02770 73.864 176 30 2 38 197 33 208 5.39e-87 255

Find 74 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTTGGGCTTTGCGTAAA+AGG 0.172350 1.4:+80498279 None:intergenic
CGACCCGGGTTCACTTTCTC+TGG 0.253150 1.4:+80498026 None:intergenic
AAGGGGTTGGTCAGAACTTT+AGG 0.293589 1.4:-80498001 MS.gene26908:CDS
ATTTCAAAGATTTGTTCTTT+TGG 0.302487 1.4:+80498227 None:intergenic
CCTTGCTAACTTGAGAAGAT+TGG 0.306141 1.4:+80498169 None:intergenic
GGTCAGAACTTTAGGTCTAT+TGG 0.344103 1.4:-80497993 MS.gene26908:CDS
AGCCTAAGACTGAGAGGGTT+TGG 0.354319 1.4:+80498428 None:intergenic
AGAGATACGGTCATTAGGTT+TGG 0.376453 1.4:+80498490 None:intergenic
CTCTTCACCCCTCCTCTCTC+TGG 0.385923 1.4:-80498515 MS.gene26908:CDS
AAAGAACAATGTTTGGCTCT+TGG 0.389124 1.4:-80498146 MS.gene26908:CDS
GGTACAGAGATACGGTCATT+AGG 0.394276 1.4:+80498485 None:intergenic
AGCAAGGAAAGAACAATGTT+TGG 0.394375 1.4:-80498153 MS.gene26908:CDS
CTTGCTAACTTGAGAAGATT+GGG 0.409133 1.4:+80498170 None:intergenic
TGGGAAACACTCTATAGAAT+TGG 0.417334 1.4:+80498090 None:intergenic
CCACTGAACTTAACCTCTAT+AGG 0.417689 1.4:+80497957 None:intergenic
GAGATACGGTCATTAGGTTT+GGG 0.426468 1.4:+80498491 None:intergenic
TCTGGGTACACACCATCCTT+AGG 0.434810 1.4:+80498044 None:intergenic
TGACCAACCCCTTGGCGACC+CGG 0.439253 1.4:+80498011 None:intergenic
TCTATAGAGTGTTTCCCAAT+GGG 0.441504 1.4:-80498085 MS.gene26908:CDS
AGAGAGAGGAGGGGTGAAGA+GGG 0.442080 1.4:+80498517 None:intergenic
ATCCAAACCCTCTCAGTCTT+AGG 0.461548 1.4:-80498430 MS.gene26908:CDS
AAGTATTGAACTTCCCCATT+GGG 0.462594 1.4:+80498071 None:intergenic
TACATAAAATTCCTCAACTT+GGG 0.465412 1.4:+80498265 None:intergenic
TAAGAATGTTAGTCCTATAG+AGG 0.473752 1.4:-80497970 MS.gene26908:CDS
GTTTGGGACCAGAGAGAGGA+GGG 0.477785 1.4:+80498507 None:intergenic
CAGAGAGAGGAGGGGTGAAG+AGG 0.479426 1.4:+80498516 None:intergenic
CAAATCTTTGAAATGCCTAC+TGG 0.482126 1.4:-80498218 MS.gene26908:CDS
ATGTTTGTGTCTTACTCAGG+TGG 0.482467 1.4:+80498335 None:intergenic
GACCCGGGTTCACTTTCTCT+GGG 0.486087 1.4:+80498027 None:intergenic
GAGAGGAGGGGTGAAGAGGG+AGG 0.499448 1.4:+80498520 None:intergenic
AAAGTGAACCCGGGTCGCCA+AGG 0.500800 1.4:-80498020 MS.gene26908:CDS
TTACATAAAATTCCTCAACT+TGG 0.508639 1.4:+80498264 None:intergenic
TCAGGTGGGGTGAAGCCAAT+TGG 0.510396 1.4:+80498350 None:intergenic
GAAGCAACAGAGGCACCAAT+TGG 0.510520 1.4:-80498365 MS.gene26908:CDS
AACCCGGGTCGCCAAGGGGT+TGG 0.516971 1.4:-80498014 MS.gene26908:CDS
GACCAACCCCTTGGCGACCC+GGG 0.524362 1.4:+80498012 None:intergenic
AATGTGGGTGATTGTTTCCA+TGG 0.524862 1.4:+80498464 None:intergenic
TTCTATAGAGTGTTTCCCAA+TGG 0.534519 1.4:-80498086 MS.gene26908:CDS
CAAGTATTGAACTTCCCCAT+TGG 0.539423 1.4:+80498070 None:intergenic
TGTTTGTGTCTTACTCAGGT+GGG 0.540009 1.4:+80498336 None:intergenic
TGGATTGGTGAAGGACAATG+TGG 0.545639 1.4:+80498448 None:intergenic
TGGCTCTTGGAACTACATTG+AGG 0.549525 1.4:-80498133 MS.gene26908:CDS
AAGTGAACCCGGGTCGCCAA+GGG 0.549528 1.4:-80498019 MS.gene26908:CDS
GTTTCCATGGTACAGAGATA+CGG 0.551001 1.4:+80498477 None:intergenic
GGTTTGGGACCAGAGAGAGG+AGG 0.559759 1.4:+80498506 None:intergenic
ATTGGGACCTTCCCTCATGA+TGG 0.564788 1.4:+80498187 None:intergenic
GTACCCAGAGAAAGTGAACC+CGG 0.564935 1.4:-80498030 MS.gene26908:CDS
GAGAGGGTTTGGATTGGTGA+AGG 0.565450 1.4:+80498439 None:intergenic
CAAAACAACAACTGTTACTG+AGG 0.567600 1.4:-80498387 MS.gene26908:CDS
TAAAGTTCTGACCAACCCCT+TGG 0.570035 1.4:+80498003 None:intergenic
AGTTCAATACTTGCATCCTA+AGG 0.578655 1.4:-80498060 MS.gene26908:CDS
GTTTGTGTCTTACTCAGGTG+GGG 0.582440 1.4:+80498337 None:intergenic
CCAATCTTCTCAAGTTAGCA+AGG 0.583877 1.4:-80498169 MS.gene26908:CDS
CAATACTTGCATCCTAAGGA+TGG 0.585635 1.4:-80498056 MS.gene26908:CDS
AAGACTGAGAGGGTTTGGAT+TGG 0.585730 1.4:+80498433 None:intergenic
CTGGTGGTGCAGCCATCATG+AGG 0.591206 1.4:-80498199 MS.gene26908:CDS
GAGGGAGGTTTGAGTTGCCG+TGG 0.592804 1.4:+80498535 None:intergenic
CCTATAGAGGTTAAGTTCAG+TGG 0.595489 1.4:-80497957 MS.gene26908:CDS
AAACCACCTCACAATGGCCA+CGG 0.601200 1.4:-80498552 None:intergenic
TTAGGTTTGGGACCAGAGAG+AGG 0.606251 1.4:+80498503 None:intergenic
TTTGGGACCAGAGAGAGGAG+GGG 0.614499 1.4:+80498508 None:intergenic
GGTGCAGCCATCATGAGGGA+AGG 0.635464 1.4:-80498194 MS.gene26908:CDS
TTGTGAGCCTAAGACTGAGA+GGG 0.636675 1.4:+80498423 None:intergenic
ATGATGGCTGCACCACCAGT+AGG 0.647307 1.4:+80498203 None:intergenic
TTTACGCAAAGCCCAAGTTG+AGG 0.649694 1.4:-80498276 MS.gene26908:CDS
CTATAGAGTGTTTCCCAATG+GGG 0.656977 1.4:-80498084 MS.gene26908:CDS
AGTGAACCCGGGTCGCCAAG+GGG 0.658596 1.4:-80498018 MS.gene26908:CDS
ATCTTTGAAATGCCTACTGG+TGG 0.664160 1.4:-80498215 MS.gene26908:CDS
TGTTACTGAGGAAGCAACAG+AGG 0.671184 1.4:-80498375 MS.gene26908:CDS
TGGTGGTGCAGCCATCATGA+GGG 0.675901 1.4:-80498198 MS.gene26908:CDS
ATTGTGAGCCTAAGACTGAG+AGG 0.683773 1.4:+80498422 None:intergenic
TACCCAGAGAAAGTGAACCC+GGG 0.686978 1.4:-80498029 MS.gene26908:CDS
ATGACCGTATCTCTGTACCA+TGG 0.695290 1.4:-80498481 MS.gene26908:CDS
GGATTGGTGAAGGACAATGT+GGG 0.717553 1.4:+80498449 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCAAAGATTTGTTCTTT+TGG + chr1.4:80498250-80498269 None:intergenic 20.0%
! TACATAAAATTCCTCAACTT+GGG + chr1.4:80498212-80498231 None:intergenic 25.0%
! TTACATAAAATTCCTCAACT+TGG + chr1.4:80498213-80498232 None:intergenic 25.0%
!! AGGAATTTTATGTAATCACA+TGG - chr1.4:80498218-80498237 MS.gene26908:CDS 25.0%
!! GGAATTTTATGTAATCACAT+GGG - chr1.4:80498219-80498238 MS.gene26908:CDS 25.0%
TAAGAATGTTAGTCCTATAG+AGG - chr1.4:80498504-80498523 MS.gene26908:CDS 30.0%
AGCAAGGAAAGAACAATGTT+TGG - chr1.4:80498321-80498340 MS.gene26908:intron 35.0%
AGTTCAATACTTGCATCCTA+AGG - chr1.4:80498414-80498433 MS.gene26908:CDS 35.0%
CAAAACAACAACTGTTACTG+AGG - chr1.4:80498087-80498106 MS.gene26908:CDS 35.0%
CTTGCTAACTTGAGAAGATT+GGG + chr1.4:80498307-80498326 None:intergenic 35.0%
TGGGAAACACTCTATAGAAT+TGG + chr1.4:80498387-80498406 None:intergenic 35.0%
! TCTATAGAGTGTTTCCCAAT+GGG - chr1.4:80498389-80498408 MS.gene26908:CDS 35.0%
! TTCTATAGAGTGTTTCCCAA+TGG - chr1.4:80498388-80498407 MS.gene26908:CDS 35.0%
!! AAAGAACAATGTTTGGCTCT+TGG - chr1.4:80498328-80498347 MS.gene26908:intron 35.0%
!! AAGTATTGAACTTCCCCATT+GGG + chr1.4:80498406-80498425 None:intergenic 35.0%
!! CAAATCTTTGAAATGCCTAC+TGG - chr1.4:80498256-80498275 MS.gene26908:CDS 35.0%
AATGTGGGTGATTGTTTCCA+TGG + chr1.4:80498013-80498032 None:intergenic 40.0%
AGAGATACGGTCATTAGGTT+TGG + chr1.4:80497987-80498006 None:intergenic 40.0%
AGTACTGCCACTGAAAATTG+GGG + chr1.4:80498170-80498189 None:intergenic 40.0%
ATGTTTGTGTCTTACTCAGG+TGG + chr1.4:80498142-80498161 None:intergenic 40.0%
CAATACTTGCATCCTAAGGA+TGG - chr1.4:80498418-80498437 MS.gene26908:CDS 40.0%
CAGTACTGCCACTGAAAATT+GGG + chr1.4:80498171-80498190 None:intergenic 40.0%
CCAATCTTCTCAAGTTAGCA+AGG - chr1.4:80498305-80498324 MS.gene26908:intron 40.0%
CCACTGAACTTAACCTCTAT+AGG + chr1.4:80498520-80498539 None:intergenic 40.0%
CCTATAGAGGTTAAGTTCAG+TGG - chr1.4:80498517-80498536 MS.gene26908:CDS 40.0%
CCTTGCTAACTTGAGAAGAT+TGG + chr1.4:80498308-80498327 None:intergenic 40.0%
GAGATACGGTCATTAGGTTT+GGG + chr1.4:80497986-80498005 None:intergenic 40.0%
GGAATGTTTGTGTCTTACTC+AGG + chr1.4:80498145-80498164 None:intergenic 40.0%
GGTCAGAACTTTAGGTCTAT+TGG - chr1.4:80498481-80498500 MS.gene26908:CDS 40.0%
GTTTCCATGGTACAGAGATA+CGG + chr1.4:80498000-80498019 None:intergenic 40.0%
TGTTTGTGTCTTACTCAGGT+GGG + chr1.4:80498141-80498160 None:intergenic 40.0%
! CTATAGAGTGTTTCCCAATG+GGG - chr1.4:80498390-80498409 MS.gene26908:CDS 40.0%
!! ATCTTTGAAATGCCTACTGG+TGG - chr1.4:80498259-80498278 MS.gene26908:CDS 40.0%
!! CAAGTATTGAACTTCCCCAT+TGG + chr1.4:80498407-80498426 None:intergenic 40.0%
AAGACTGAGAGGGTTTGGAT+TGG + chr1.4:80498044-80498063 None:intergenic 45.0%
ATCCAAACCCTCTCAGTCTT+AGG - chr1.4:80498044-80498063 MS.gene26908:CDS 45.0%
ATGACCGTATCTCTGTACCA+TGG - chr1.4:80497993-80498012 MS.gene26908:CDS 45.0%
ATTGTGAGCCTAAGACTGAG+AGG + chr1.4:80498055-80498074 None:intergenic 45.0%
CAACTTGGGCTTTGCGTAAA+AGG + chr1.4:80498198-80498217 None:intergenic 45.0%
CCAGTACTGCCACTGAAAAT+TGG + chr1.4:80498172-80498191 None:intergenic 45.0%
GGTACAGAGATACGGTCATT+AGG + chr1.4:80497992-80498011 None:intergenic 45.0%
GTTTGTGTCTTACTCAGGTG+GGG + chr1.4:80498140-80498159 None:intergenic 45.0%
TGGCTCTTGGAACTACATTG+AGG - chr1.4:80498341-80498360 MS.gene26908:intron 45.0%
TGTTACTGAGGAAGCAACAG+AGG - chr1.4:80498099-80498118 MS.gene26908:CDS 45.0%
TTGTGAGCCTAAGACTGAGA+GGG + chr1.4:80498054-80498073 None:intergenic 45.0%
TTTACGCAAAGCCCAAGTTG+AGG - chr1.4:80498198-80498217 MS.gene26908:CDS 45.0%
! ATTCCCTCCCCAATTTTCAG+TGG - chr1.4:80498160-80498179 MS.gene26908:CDS 45.0%
! CCAATTTTCAGTGGCAGTAC+TGG - chr1.4:80498169-80498188 MS.gene26908:CDS 45.0%
! GGATTGGTGAAGGACAATGT+GGG + chr1.4:80498028-80498047 None:intergenic 45.0%
! TAAAGTTCTGACCAACCCCT+TGG + chr1.4:80498474-80498493 None:intergenic 45.0%
!! AAGGGGTTGGTCAGAACTTT+AGG - chr1.4:80498473-80498492 MS.gene26908:CDS 45.0%
!! ATTTTCAGTGGCAGTACTGG+AGG - chr1.4:80498172-80498191 MS.gene26908:CDS 45.0%
!! TGGATTGGTGAAGGACAATG+TGG + chr1.4:80498029-80498048 None:intergenic 45.0%
ACTGCCACTGAAAATTGGGG+AGG + chr1.4:80498167-80498186 None:intergenic 50.0%
AGCCTAAGACTGAGAGGGTT+TGG + chr1.4:80498049-80498068 None:intergenic 50.0%
ATTGGGACCTTCCCTCATGA+TGG + chr1.4:80498290-80498309 None:intergenic 50.0%
CTGCCACTGAAAATTGGGGA+GGG + chr1.4:80498166-80498185 None:intergenic 50.0%
GTACCCAGAGAAAGTGAACC+CGG - chr1.4:80498444-80498463 MS.gene26908:CDS 50.0%
TACCCAGAGAAAGTGAACCC+GGG - chr1.4:80498445-80498464 MS.gene26908:CDS 50.0%
TCTGGGTACACACCATCCTT+AGG + chr1.4:80498433-80498452 None:intergenic 50.0%
! TTAGGTTTGGGACCAGAGAG+AGG + chr1.4:80497974-80497993 None:intergenic 50.0%
!! GAAGCAACAGAGGCACCAAT+TGG - chr1.4:80498109-80498128 MS.gene26908:CDS 50.0%
!! GAGAGGGTTTGGATTGGTGA+AGG + chr1.4:80498038-80498057 None:intergenic 50.0%
AGAGAGAGGAGGGGTGAAGA+GGG + chr1.4:80497960-80497979 None:intergenic 55.0%
ATGATGGCTGCACCACCAGT+AGG + chr1.4:80498274-80498293 None:intergenic 55.0%
GACCCGGGTTCACTTTCTCT+GGG + chr1.4:80498450-80498469 None:intergenic 55.0%
TCAGGTGGGGTGAAGCCAAT+TGG + chr1.4:80498127-80498146 None:intergenic 55.0%
! GTTTGGGACCAGAGAGAGGA+GGG + chr1.4:80497970-80497989 None:intergenic 55.0%
! TGGTGGTGCAGCCATCATGA+GGG - chr1.4:80498276-80498295 MS.gene26908:CDS 55.0%
! TTTGGGACCAGAGAGAGGAG+GGG + chr1.4:80497969-80497988 None:intergenic 55.0%
AAAGTGAACCCGGGTCGCCA+AGG - chr1.4:80498454-80498473 MS.gene26908:CDS 60.0%
AAGTGAACCCGGGTCGCCAA+GGG - chr1.4:80498455-80498474 MS.gene26908:CDS 60.0%
CAGAGAGAGGAGGGGTGAAG+AGG + chr1.4:80497961-80497980 None:intergenic 60.0%
CGACCCGGGTTCACTTTCTC+TGG + chr1.4:80498451-80498470 None:intergenic 60.0%
CTCTTCACCCCTCCTCTCTC+TGG - chr1.4:80497959-80497978 MS.gene26908:CDS 60.0%
GGTGCAGCCATCATGAGGGA+AGG - chr1.4:80498280-80498299 MS.gene26908:CDS 60.0%
! CTGGTGGTGCAGCCATCATG+AGG - chr1.4:80498275-80498294 MS.gene26908:CDS 60.0%
! GGTTTGGGACCAGAGAGAGG+AGG + chr1.4:80497971-80497990 None:intergenic 60.0%
!! GAGGGAGGTTTGAGTTGCCG+TGG + chr1.4:80497942-80497961 None:intergenic 60.0%
AGTGAACCCGGGTCGCCAAG+GGG - chr1.4:80498456-80498475 MS.gene26908:CDS 65.0%
GAGAGGAGGGGTGAAGAGGG+AGG + chr1.4:80497957-80497976 None:intergenic 65.0%
TGACCAACCCCTTGGCGACC+CGG + chr1.4:80498466-80498485 None:intergenic 65.0%
AACCCGGGTCGCCAAGGGGT+TGG - chr1.4:80498460-80498479 MS.gene26908:CDS 70.0%
GACCAACCCCTTGGCGACCC+GGG + chr1.4:80498465-80498484 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 80497935 80498561 80497935 ID=MS.gene26908
chr1.4 mRNA 80497935 80498561 80497935 ID=MS.gene26908.t1;Parent=MS.gene26908
chr1.4 exon 80498350 80498561 80498350 ID=MS.gene26908.t1.exon1;Parent=MS.gene26908.t1
chr1.4 CDS 80498350 80498561 80498350 ID=cds.MS.gene26908.t1;Parent=MS.gene26908.t1
chr1.4 exon 80497935 80498316 80497935 ID=MS.gene26908.t1.exon2;Parent=MS.gene26908.t1
chr1.4 CDS 80497935 80498316 80497935 ID=cds.MS.gene26908.t1;Parent=MS.gene26908.t1
Gene Sequence

>MS.gene26908

ATGGCCACGGCAACTCAAACCTCCCTCTTCACCCCTCCTCTCTCTGGTCCCAAACCTAATGACCGTATCTCTGTACCATGGAAACAATCACCCACATTGTCCTTCACCAATCCAAACCCTCTCAGTCTTAGGCTCACAATTTCAAGAAACATCAAAACAACAACTGTTACTGAGGAAGCAACAGAGGCACCAATTGGCTTCACCCCACCTGAGTAAGACACAAACATTCCCTCCCCAATTTTCAGTGGCAGTACTGGAGGCCTTTTACGCAAAGCCCAAGTTGAGGAATTTTATGTAATCACATGGGATTCACCAAAAGAACAAATCTTTGAAATGCCTACTGGTGGTGCAGCCATCATGAGGGAAGGTCCCAATCTTCTCAAGTTAGCAAGGAAAGAACAATGTTTGGCTCTTGGAACTACATTGAGGTCAAAGTACAAGATCAAGTACCAATTCTATAGAGTGTTTCCCAATGGGGAAGTTCAATACTTGCATCCTAAGGATGGTGTGTACCCAGAGAAAGTGAACCCGGGTCGCCAAGGGGTTGGTCAGAACTTTAGGTCTATTGGTAAGAATGTTAGTCCTATAGAGGTTAAGTTCAGTGGCAAGCAAGTGCATGAATTGTGA

Protein sequence

>MS.gene26908.t1

MATATQTSLFTPPLSGPKPNDRISVPWKQSPTLSFTNPNPLSLRLTISRNIKTTTVTEEATEAPIGFTPPDGSTGGLLRKAQVEEFYVITWDSPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTTLRSKYKIKYQFYRVFPNGEVQYLHPKDGVYPEKVNPGRQGVGQNFRSIGKNVSPIEVKFSGKQVHEL