Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27099.t1 | XP_003589562.1 | 93.2 | 249 | 15 | 1 | 1 | 249 | 1 | 247 | 3.80E-130 | 474.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27099.t1 | Q9SGS5 | 30.9 | 282 | 133 | 9 | 4 | 245 | 13 | 272 | 2.0e-18 | 94.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27099.t1 | G7I8D2 | 93.2 | 249 | 15 | 1 | 1 | 249 | 1 | 247 | 2.7e-130 | 474.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene27099 | 0.859369 | 4.34E-63 | -1.69E-46 |
| MS.gene049457 | MS.gene27099 | 0.809115 | 2.22E-50 | -1.69E-46 |
| MS.gene050363 | MS.gene27099 | 0.838139 | 3.43E-57 | -1.69E-46 |
| MS.gene050416 | MS.gene27099 | 0.812698 | 3.72E-51 | -1.69E-46 |
| MS.gene050526 | MS.gene27099 | 0.802698 | 4.96E-49 | -1.69E-46 |
| MS.gene050883 | MS.gene27099 | 0.817591 | 3.05E-52 | -1.69E-46 |
| MS.gene051959 | MS.gene27099 | 0.823048 | 1.71E-53 | -1.69E-46 |
| MS.gene051960 | MS.gene27099 | 0.82441 | 8.19E-54 | -1.69E-46 |
| MS.gene052053 | MS.gene27099 | 0.819868 | 9.27E-53 | -1.69E-46 |
| MS.gene052091 | MS.gene27099 | 0.848754 | 5.01E-60 | -1.69E-46 |
| MS.gene053549 | MS.gene27099 | 0.805648 | 1.21E-49 | -1.69E-46 |
| MS.gene053932 | MS.gene27099 | 0.869401 | 3.18E-66 | -1.69E-46 |
| MS.gene055549 | MS.gene27099 | 0.87144 | 6.82E-67 | -1.69E-46 |
| MS.gene055550 | MS.gene27099 | 0.877776 | 4.79E-69 | -1.69E-46 |
| MS.gene055551 | MS.gene27099 | 0.862812 | 3.89E-64 | -1.69E-46 |
| MS.gene056311 | MS.gene27099 | 0.812611 | 3.89E-51 | -1.69E-46 |
| MS.gene056365 | MS.gene27099 | 0.817803 | 2.73E-52 | -1.69E-46 |
| MS.gene056408 | MS.gene27099 | 0.828151 | 1.05E-54 | -1.69E-46 |
| MS.gene056530 | MS.gene27099 | 0.852516 | 4.39E-61 | -1.69E-46 |
| MS.gene057001 | MS.gene27099 | 0.810359 | 1.20E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene27099 | 0.801911 | 7.20E-49 | -1.69E-46 |
| MS.gene057093 | MS.gene27099 | 0.820617 | 6.24E-53 | -1.69E-46 |
| MS.gene058628 | MS.gene27099 | 0.830472 | 2.88E-55 | -1.69E-46 |
| MS.gene058629 | MS.gene27099 | 0.835038 | 2.12E-56 | -1.69E-46 |
| MS.gene058630 | MS.gene27099 | 0.831051 | 2.08E-55 | -1.69E-46 |
| MS.gene060237 | MS.gene27099 | 0.83075 | 2.46E-55 | -1.69E-46 |
| MS.gene060964 | MS.gene27099 | 0.811282 | 7.57E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27099.t1 | MTR_1g026110 | 93.173 | 249 | 15 | 1 | 1 | 249 | 1 | 247 | 2.58e-165 | 457 |
| MS.gene27099.t1 | MTR_3g114750 | 49.808 | 261 | 94 | 12 | 4 | 245 | 2 | 244 | 1.02e-59 | 189 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27099.t1 | AT1G76070 | 32.472 | 271 | 121 | 10 | 15 | 245 | 24 | 272 | 1.74e-26 | 104 |
| MS.gene27099.t1 | AT1G20310 | 33.210 | 271 | 119 | 10 | 1 | 242 | 1 | 238 | 3.51e-26 | 102 |
Find 58 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATATCTTTGTTGGGTTT+TGG | 0.225407 | 1.1:+274145 | None:intergenic |
| TCCCTTTGATCAATTTCTTC+AGG | 0.304197 | 1.1:+273914 | None:intergenic |
| TTCACCAAAGATTTCTTGTA+TGG | 0.307383 | 1.1:-274200 | MS.gene27099:CDS |
| TCATTGCCACCACCATCTTT+AGG | 0.317528 | 1.1:+274238 | None:intergenic |
| CCTAAAATAGGTGCAGAAAA+TGG | 0.326900 | 1.1:+273854 | None:intergenic |
| TTTCCACCAACACCTAAAAT+AGG | 0.335034 | 1.1:+273842 | None:intergenic |
| CTAAATTTGTGATCTCTTCC+TGG | 0.339147 | 1.1:+274364 | None:intergenic |
| TTTACCTCCGTATCCACCTT+TGG | 0.373153 | 1.1:+274118 | None:intergenic |
| TGGAGTGAAGATATCTTTGT+TGG | 0.381588 | 1.1:+274138 | None:intergenic |
| GAAAGGAAATCAGATGGTTC+TGG | 0.386986 | 1.1:-274043 | MS.gene27099:CDS |
| CAAGAAATCTTTGGTGAAGT+AGG | 0.393822 | 1.1:+274205 | None:intergenic |
| CAAGGAATGAGATTAATCTT+TGG | 0.407911 | 1.1:-273781 | MS.gene27099:CDS |
| TTAGGTGTTGGTGGAAATAG+TGG | 0.423953 | 1.1:-273836 | MS.gene27099:CDS |
| TGTCCCATACAAGAAATCTT+TGG | 0.426848 | 1.1:+274196 | None:intergenic |
| GCCTGAAGAAATTGATCAAA+GGG | 0.432040 | 1.1:-273915 | MS.gene27099:CDS |
| CAAAGATTAATCTCATTCCT+TGG | 0.442885 | 1.1:+273782 | None:intergenic |
| GGAGTGAAGATATCTTTGTT+GGG | 0.452969 | 1.1:+274139 | None:intergenic |
| CAAAACGCTCCTTTCAGTCC+AGG | 0.469371 | 1.1:-274382 | MS.gene27099:CDS |
| CTTCACTCCAAAGGTGGATA+CGG | 0.475485 | 1.1:-274125 | MS.gene27099:CDS |
| AGAAAATAACACAAAGTCAT+GGG | 0.481776 | 1.1:-274338 | MS.gene27099:CDS |
| GGTTCTGGATTTGTTGATGA+AGG | 0.482613 | 1.1:-274028 | MS.gene27099:CDS |
| CAAAGGGATTGCTTTACTGA+TGG | 0.491176 | 1.1:-273899 | MS.gene27099:CDS |
| CACCGCCGATGGGTGATATG+AGG | 0.497283 | 1.1:-273994 | MS.gene27099:CDS |
| GGATTCAACTGAAGAGGCCT+AGG | 0.502469 | 1.1:+273737 | None:intergenic |
| ATTTATTTAGCTTTAAGCAC+AGG | 0.503675 | 1.1:+273716 | None:intergenic |
| AAAACTCATGCTACTGGTAA+GGG | 0.508213 | 1.1:-274310 | MS.gene27099:CDS |
| CTGATTTGAAGTTTAAGCCA+AGG | 0.518893 | 1.1:-273799 | MS.gene27099:CDS |
| TGATCTCTTCCTGGACTGAA+AGG | 0.521071 | 1.1:+274373 | None:intergenic |
| CAGAAAATAACACAAAGTCA+TGG | 0.527453 | 1.1:-274339 | MS.gene27099:CDS |
| AAGAGAAACAAAGTAAGCTA+AGG | 0.530956 | 1.1:-274438 | MS.gene27099:CDS |
| GCTAGAAGAAAACCTAAAGA+TGG | 0.536495 | 1.1:-274250 | MS.gene27099:CDS |
| AGCCTGAAGAAATTGATCAA+AGG | 0.541841 | 1.1:-273916 | MS.gene27099:CDS |
| TAAAGAGATCGAAGTTTCAG+AGG | 0.548970 | 1.1:-274099 | MS.gene27099:CDS |
| AACAACAAAGCACCGCCGAT+GGG | 0.567253 | 1.1:-274004 | MS.gene27099:CDS |
| AGCACAGGATTCAACTGAAG+AGG | 0.568748 | 1.1:+273731 | None:intergenic |
| TCACCAAAGATTTCTTGTAT+GGG | 0.570550 | 1.1:-274199 | MS.gene27099:CDS |
| GTTCTGGATTTGTTGATGAA+GGG | 0.581024 | 1.1:-274027 | MS.gene27099:CDS |
| AGAAAACCTAAAGATGGTGG+TGG | 0.587690 | 1.1:-274244 | MS.gene27099:CDS |
| TATGGAATCATAGTAACCAT+AGG | 0.589627 | 1.1:+274277 | None:intergenic |
| CAAAGATATCTTCACTCCAA+AGG | 0.590991 | 1.1:-274134 | MS.gene27099:CDS |
| GAACAACAAAGCACCGCCGA+TGG | 0.591209 | 1.1:-274005 | MS.gene27099:CDS |
| GAGATTTGCAAGTGGACGTG+AGG | 0.598149 | 1.1:-273972 | MS.gene27099:CDS |
| TTTGCGGAAAGGAAATCAGA+TGG | 0.598533 | 1.1:-274049 | MS.gene27099:CDS |
| TTGCTTTACTGATGGAGAGG+AGG | 0.603818 | 1.1:-273891 | MS.gene27099:CDS |
| GGATTGCTTTACTGATGGAG+AGG | 0.611817 | 1.1:-273894 | MS.gene27099:CDS |
| CTAAAGCGAAATTTGCGGAA+AGG | 0.612185 | 1.1:-274060 | MS.gene27099:CDS |
| AGATATCTTCACTCCAAAGG+TGG | 0.612984 | 1.1:-274131 | MS.gene27099:CDS |
| GAAGAACAATGGCTCCACCT+AGG | 0.627085 | 1.1:-273754 | MS.gene27099:CDS |
| TTCAACTGAAGAGGCCTAGG+TGG | 0.634353 | 1.1:+273740 | None:intergenic |
| AGAAGAAAACCTAAAGATGG+TGG | 0.634473 | 1.1:-274247 | MS.gene27099:CDS |
| TCTTTGGAAGAGAAGAACAA+TGG | 0.638654 | 1.1:-273765 | MS.gene27099:CDS |
| CACTCCAAAGGTGGATACGG+AGG | 0.642812 | 1.1:-274122 | MS.gene27099:CDS |
| AATCTCCTCATATCACCCAT+CGG | 0.643282 | 1.1:+273989 | None:intergenic |
| GAAAATAACACAAAGTCATG+GGG | 0.645047 | 1.1:-274337 | MS.gene27099:CDS |
| AAATAGTGGTACATCTAATG+TGG | 0.671533 | 1.1:-273822 | MS.gene27099:CDS |
| CTCCTCATATCACCCATCGG+CGG | 0.680805 | 1.1:+273992 | None:intergenic |
| GAGGTCTAAAGCGAAATTTG+CGG | 0.681767 | 1.1:-274065 | MS.gene27099:CDS |
| GATATGAGGAGATTTGCAAG+TGG | 0.723470 | 1.1:-273980 | MS.gene27099:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTCTAAATTTGATTGGA+AGG | - | chr1.1:274215-274234 | MS.gene27099:CDS | 20.0% |
| ! | AGAAAATAACACAAAGTCAT+GGG | - | chr1.1:273825-273844 | MS.gene27099:CDS | 25.0% |
| !!! | AGGCTTTTTCTAAATTTGAT+TGG | - | chr1.1:274211-274230 | MS.gene27099:CDS | 25.0% |
| AAATAGTGGTACATCTAATG+TGG | - | chr1.1:274341-274360 | MS.gene27099:CDS | 30.0% | |
| AAGAGAAACAAAGTAAGCTA+AGG | - | chr1.1:273725-273744 | MS.gene27099:CDS | 30.0% | |
| CAAAGATTAATCTCATTCCT+TGG | + | chr1.1:274384-274403 | None:intergenic | 30.0% | |
| CAAGGAATGAGATTAATCTT+TGG | - | chr1.1:274382-274401 | MS.gene27099:CDS | 30.0% | |
| CAGAAAATAACACAAAGTCA+TGG | - | chr1.1:273824-273843 | MS.gene27099:CDS | 30.0% | |
| GAAAATAACACAAAGTCATG+GGG | - | chr1.1:273826-273845 | MS.gene27099:CDS | 30.0% | |
| TATGGAATCATAGTAACCAT+AGG | + | chr1.1:273889-273908 | None:intergenic | 30.0% | |
| TCACCAAAGATTTCTTGTAT+GGG | - | chr1.1:273964-273983 | MS.gene27099:CDS | 30.0% | |
| TTCACCAAAGATTTCTTGTA+TGG | - | chr1.1:273963-273982 | MS.gene27099:CDS | 30.0% | |
| ! | AAGATATCTTTGTTGGGTTT+TGG | + | chr1.1:274021-274040 | None:intergenic | 30.0% |
| AAAAACTCATGCTACTGGTA+AGG | - | chr1.1:273852-273871 | MS.gene27099:CDS | 35.0% | |
| AAAACTCATGCTACTGGTAA+GGG | - | chr1.1:273853-273872 | MS.gene27099:CDS | 35.0% | |
| AGAAGAAAACCTAAAGATGG+TGG | - | chr1.1:273916-273935 | MS.gene27099:CDS | 35.0% | |
| AGCCTGAAGAAATTGATCAA+AGG | - | chr1.1:274247-274266 | MS.gene27099:CDS | 35.0% | |
| CAAAGATATCTTCACTCCAA+AGG | - | chr1.1:274029-274048 | MS.gene27099:CDS | 35.0% | |
| CAAGAAATCTTTGGTGAAGT+AGG | + | chr1.1:273961-273980 | None:intergenic | 35.0% | |
| CCTAAAATAGGTGCAGAAAA+TGG | + | chr1.1:274312-274331 | None:intergenic | 35.0% | |
| CTAAATTTGTGATCTCTTCC+TGG | + | chr1.1:273802-273821 | None:intergenic | 35.0% | |
| GCCTGAAGAAATTGATCAAA+GGG | - | chr1.1:274248-274267 | MS.gene27099:CDS | 35.0% | |
| GCTAGAAGAAAACCTAAAGA+TGG | - | chr1.1:273913-273932 | MS.gene27099:CDS | 35.0% | |
| GGAGTGAAGATATCTTTGTT+GGG | + | chr1.1:274027-274046 | None:intergenic | 35.0% | |
| TAAAGAGATCGAAGTTTCAG+AGG | - | chr1.1:274064-274083 | MS.gene27099:CDS | 35.0% | |
| TCCCTTTGATCAATTTCTTC+AGG | + | chr1.1:274252-274271 | None:intergenic | 35.0% | |
| TGGAGTGAAGATATCTTTGT+TGG | + | chr1.1:274028-274047 | None:intergenic | 35.0% | |
| TGTCCCATACAAGAAATCTT+TGG | + | chr1.1:273970-273989 | None:intergenic | 35.0% | |
| TTTCCACCAACACCTAAAAT+AGG | + | chr1.1:274324-274343 | None:intergenic | 35.0% | |
| ! | CCATTTTCTGCACCTATTTT+AGG | - | chr1.1:274309-274328 | MS.gene27099:CDS | 35.0% |
| ! | CTGATTTGAAGTTTAAGCCA+AGG | - | chr1.1:274364-274383 | MS.gene27099:CDS | 35.0% |
| ! | TCTTTGGAAGAGAAGAACAA+TGG | - | chr1.1:274398-274417 | MS.gene27099:CDS | 35.0% |
| !! | GTTCTGGATTTGTTGATGAA+GGG | - | chr1.1:274136-274155 | MS.gene27099:CDS | 35.0% |
| !!! | GGTTTTCTTCTAGCTTCATA+TGG | + | chr1.1:273907-273926 | None:intergenic | 35.0% |
| AATCTCCTCATATCACCCAT+CGG | + | chr1.1:274177-274196 | None:intergenic | 40.0% | |
| AGATATCTTCACTCCAAAGG+TGG | - | chr1.1:274032-274051 | MS.gene27099:CDS | 40.0% | |
| CAAAGGGATTGCTTTACTGA+TGG | - | chr1.1:274264-274283 | MS.gene27099:CDS | 40.0% | |
| CTAAAGCGAAATTTGCGGAA+AGG | - | chr1.1:274103-274122 | MS.gene27099:CDS | 40.0% | |
| GAAAGGAAATCAGATGGTTC+TGG | - | chr1.1:274120-274139 | MS.gene27099:CDS | 40.0% | |
| GATATGAGGAGATTTGCAAG+TGG | - | chr1.1:274183-274202 | MS.gene27099:CDS | 40.0% | |
| TTTGCGGAAAGGAAATCAGA+TGG | - | chr1.1:274114-274133 | MS.gene27099:CDS | 40.0% | |
| ! | AGAAAACCTAAAGATGGTGG+TGG | - | chr1.1:273919-273938 | MS.gene27099:CDS | 40.0% |
| ! | GAGGTCTAAAGCGAAATTTG+CGG | - | chr1.1:274098-274117 | MS.gene27099:CDS | 40.0% |
| ! | TAAGGGATTTTCTGGACCTA+TGG | - | chr1.1:273870-273889 | MS.gene27099:CDS | 40.0% |
| !! | GGTTCTGGATTTGTTGATGA+AGG | - | chr1.1:274135-274154 | MS.gene27099:CDS | 40.0% |
| !! | TCTGCACCTATTTTAGGTGT+TGG | - | chr1.1:274315-274334 | MS.gene27099:CDS | 40.0% |
| !! | TTAGGTGTTGGTGGAAATAG+TGG | - | chr1.1:274327-274346 | MS.gene27099:CDS | 40.0% |
| !!! | TTCAGAGGATTTTTTCGCAG+AGG | - | chr1.1:274079-274098 | MS.gene27099:CDS | 40.0% |
| AGCACAGGATTCAACTGAAG+AGG | + | chr1.1:274435-274454 | None:intergenic | 45.0% | |
| CTTCACTCCAAAGGTGGATA+CGG | - | chr1.1:274038-274057 | MS.gene27099:CDS | 45.0% | |
| GGATTGCTTTACTGATGGAG+AGG | - | chr1.1:274269-274288 | MS.gene27099:CDS | 45.0% | |
| GGTGCAAAAACTCATGCTAC+TGG | - | chr1.1:273847-273866 | MS.gene27099:CDS | 45.0% | |
| TCATTGCCACCACCATCTTT+AGG | + | chr1.1:273928-273947 | None:intergenic | 45.0% | |
| TGATCTCTTCCTGGACTGAA+AGG | + | chr1.1:273793-273812 | None:intergenic | 45.0% | |
| TTGCTTTACTGATGGAGAGG+AGG | - | chr1.1:274272-274291 | MS.gene27099:CDS | 45.0% | |
| TTTACCTCCGTATCCACCTT+TGG | + | chr1.1:274048-274067 | None:intergenic | 45.0% | |
| ! | GCTACTGGTAAGGGATTTTC+TGG | - | chr1.1:273862-273881 | MS.gene27099:CDS | 45.0% |
| !!! | GCACCTATTTTAGGTGTTGG+TGG | - | chr1.1:274318-274337 | MS.gene27099:CDS | 45.0% |
| CAAAACGCTCCTTTCAGTCC+AGG | - | chr1.1:273781-273800 | MS.gene27099:CDS | 50.0% | |
| GAAGAACAATGGCTCCACCT+AGG | - | chr1.1:274409-274428 | MS.gene27099:CDS | 50.0% | |
| GAGATTTGCAAGTGGACGTG+AGG | - | chr1.1:274191-274210 | MS.gene27099:CDS | 50.0% | |
| GGATTCAACTGAAGAGGCCT+AGG | + | chr1.1:274429-274448 | None:intergenic | 50.0% | |
| TTCAACTGAAGAGGCCTAGG+TGG | + | chr1.1:274426-274445 | None:intergenic | 50.0% | |
| !! | AACAACAAAGCACCGCCGAT+GGG | - | chr1.1:274159-274178 | MS.gene27099:CDS | 50.0% |
| CACTCCAAAGGTGGATACGG+AGG | - | chr1.1:274041-274060 | MS.gene27099:CDS | 55.0% | |
| CTCCTCATATCACCCATCGG+CGG | + | chr1.1:274174-274193 | None:intergenic | 55.0% | |
| !! | GAACAACAAAGCACCGCCGA+TGG | - | chr1.1:274158-274177 | MS.gene27099:CDS | 55.0% |
| CACCGCCGATGGGTGATATG+AGG | - | chr1.1:274169-274188 | MS.gene27099:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 273718 | 274467 | 273718 | ID=MS.gene27099 |
| chr1.1 | mRNA | 273718 | 274467 | 273718 | ID=MS.gene27099.t1;Parent=MS.gene27099 |
| chr1.1 | exon | 273718 | 274467 | 273718 | ID=MS.gene27099.t1.exon1;Parent=MS.gene27099.t1 |
| chr1.1 | CDS | 273718 | 274467 | 273718 | ID=cds.MS.gene27099.t1;Parent=MS.gene27099.t1 |
| Gene Sequence |
| Protein sequence |
