AlfalfaGEDB | MS.gene27823

M. sativa cultivar XinJiangDaYe / MS.gene27823

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27823.t1	RHN60978.1	96.4	138	4	1	1	137	1	138	2.90E-64	254.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27823.t1	Q1S053	96.2	133	5	0	1	133	1	133	9.9e-66	250.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27823.t1	A0A396I838	96.4	138	4	1	1	137	1	138	2.1e-64	254.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049397	MS.gene27823	0.802461	5.55E-49	-1.69E-46
MS.gene049596	MS.gene27823	0.8519	6.57E-61	-1.69E-46
MS.gene04972	MS.gene27823	0.837861	4.05E-57	-1.69E-46
MS.gene04985	MS.gene27823	0.856048	4.19E-62	-1.69E-46
MS.gene049989	MS.gene27823	0.854225	1.42E-61	-1.69E-46
MS.gene050297	MS.gene27823	0.883642	3.77E-71	-1.69E-46
MS.gene050648	MS.gene27823	0.826167	3.15E-54	-1.69E-46
MS.gene05124	MS.gene27823	0.845115	4.97E-59	-1.69E-46
MS.gene051263	MS.gene27823	0.820493	6.66E-53	-1.69E-46
MS.gene052367	MS.gene27823	0.833338	5.65E-56	-1.69E-46
MS.gene053173	MS.gene27823	0.825417	4.74E-54	-1.69E-46
MS.gene053416	MS.gene27823	0.84187	3.66E-58	-1.69E-46
MS.gene053417	MS.gene27823	0.840798	7.00E-58	-1.69E-46
MS.gene054551	MS.gene27823	0.819483	1.13E-52	-1.69E-46
MS.gene054643	MS.gene27823	0.873392	1.52E-67	-1.69E-46
MS.gene055411	MS.gene27823	0.829638	4.60E-55	-1.69E-46
MS.gene055957	MS.gene27823	0.869769	2.41E-66	-1.69E-46
MS.gene056041	MS.gene27823	0.822046	2.92E-53	-1.69E-46
MS.gene05640	MS.gene27823	0.805533	1.27E-49	-1.69E-46
MS.gene057458	MS.gene27823	0.812893	3.37E-51	-1.69E-46
MS.gene057483	MS.gene27823	0.806172	9.36E-50	-1.69E-46
MS.gene058818	MS.gene27823	0.981658	3.95E-153	-1.69E-46
MS.gene058844	MS.gene27823	0.869539	2.87E-66	-1.69E-46
MS.gene059300	MS.gene27823	0.83748	5.07E-57	-1.69E-46
MS.gene060473	MS.gene27823	0.826457	2.68E-54	-1.69E-46
MS.gene060565	MS.gene27823	0.82928	5.62E-55	-1.69E-46
MS.gene06057	MS.gene27823	0.850875	1.28E-60	-1.69E-46
MS.gene06093	MS.gene27823	0.814496	1.50E-51	-1.69E-46
MS.gene061260	MS.gene27823	0.844359	7.94E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27823.t1	MTR_8g086640	98.000	150	3	0	1	150	1	150	3.92e-101	286
MS.gene27823.t1	MTR_4g063280	95.455	132	5	1	20	150	14	145	1.59e-84	244
MS.gene27823.t1	MTR_2g096610	87.333	150	17	1	1	150	1	148	1.63e-83	242
MS.gene27823.t1	MTR_4g064967	94.000	150	7	1	1	150	1	148	2.88e-81	236
MS.gene27823.t1	MTR_4g071150	91.503	153	9	2	1	150	1	152	8.92e-80	233
MS.gene27823.t1	MTR_4g064005	91.333	150	11	1	1	150	1	148	4.09e-79	231
MS.gene27823.t1	MTR_4g063410	90.667	150	12	1	1	150	1	148	9.41e-79	230
MS.gene27823.t1	MTR_2g096570	89.610	154	11	3	1	150	1	153	6.78e-78	228
MS.gene27823.t1	MTR_7g113160	81.955	133	23	1	10	142	13	144	2.68e-72	213
MS.gene27823.t1	MTR_7g108320	70.213	141	39	2	3	142	2	140	1.69e-60	183
MS.gene27823.t1	MTR_7g114060	70.000	140	38	1	3	142	2	137	7.19e-60	182
MS.gene27823.t1	MTR_7g114040	70.000	140	38	1	3	142	2	137	7.19e-60	182
MS.gene27823.t1	MTR_2g082510	74.790	119	30	0	11	129	3	121	1.62e-58	178
MS.gene27823.t1	MTR_7g093910	72.222	126	32	1	4	129	5	127	2.60e-58	178
MS.gene27823.t1	MTR_2g082220	73.950	119	31	0	11	129	3	121	2.40e-57	175
MS.gene27823.t1	MTR_6g005150	64.567	127	31	3	24	149	26	139	6.55e-48	151
MS.gene27823.t1	MTR_7g114050	65.385	130	30	3	17	142	10	128	2.08e-46	147
MS.gene27823.t1	MTR_4g063300	76.190	105	20	1	45	149	10	109	7.34e-46	145
MS.gene27823.t1	MTR_1g011750	59.292	113	44	2	21	132	23	134	5.99e-36	121
MS.gene27823.t1	MTR_1g011850	59.292	113	44	2	21	132	23	134	5.99e-36	121
MS.gene27823.t1	MTR_1g114050	59.633	109	42	2	21	128	20	127	5.83e-35	118
MS.gene27823.t1	MTR_1g071040	61.538	104	38	2	26	128	28	130	2.86e-34	117
MS.gene27823.t1	MTR_1g069770	53.333	90	41	1	45	133	9	98	1.46e-24	91.3
MS.gene27823.t1	MTR_7g010440	70.588	51	15	0	78	128	122	172	3.09e-16	73.9
MS.gene27823.t1	MTR_4g048490	43.810	105	30	2	24	128	26	101	3.42e-16	70.1
MS.gene27823.t1	MTR_4g005910	74.419	43	11	0	87	129	4	46	5.10e-16	68.2
MS.gene27823.t1	MTR_7g027610	40.517	116	43	3	20	134	10	100	3.12e-15	67.8
MS.gene27823.t1	MTR_2g096590	72.500	40	11	0	110	149	12	51	3.03e-12	58.2
MS.gene27823.t1	MTR_0653s0030	53.704	54	25	0	56	109	44	97	1.38e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene27823.t1	AT5G02560	82.353	153	24	1	1	150	1	153	2.22e-80	234
MS.gene27823.t1	AT5G02560	71.186	177	24	2	1	150	1	177	2.21e-75	223
MS.gene27823.t1	AT5G27670	75.000	152	34	3	1	150	1	150	1.39e-72	214
MS.gene27823.t1	AT5G59870	75.000	148	36	1	4	150	3	150	8.98e-72	212
MS.gene27823.t1	AT1G51060	76.667	120	28	0	11	130	3	122	9.15e-61	184
MS.gene27823.t1	AT4G27230	75.630	119	29	0	11	129	3	121	1.76e-59	181
MS.gene27823.t1	AT4G27230	75.630	119	29	0	11	129	3	121	1.76e-59	181
MS.gene27823.t1	AT3G20670	75.630	119	29	0	11	129	3	121	7.39e-59	179
MS.gene27823.t1	AT1G08880	71.318	129	34	1	7	132	2	130	1.84e-58	178
MS.gene27823.t1	AT5G54640	74.790	119	30	0	11	129	3	121	4.41e-58	177
MS.gene27823.t1	AT1G54690	72.800	125	31	1	11	132	6	130	8.23e-58	177
MS.gene27823.t1	AT2G38810	59.821	112	43	2	21	131	25	135	1.96e-36	122
MS.gene27823.t1	AT2G38810	59.821	112	43	2	21	131	25	135	1.96e-36	122
MS.gene27823.t1	AT2G38810	59.821	112	43	2	21	131	25	135	1.96e-36	122
MS.gene27823.t1	AT2G38810	59.821	112	43	2	21	131	25	135	1.96e-36	122
MS.gene27823.t1	AT1G52740	59.633	109	42	2	25	132	27	134	1.08e-34	118
MS.gene27823.t1	AT3G54560	58.716	109	43	2	25	132	29	136	1.73e-34	117
MS.gene27823.t1	AT3G54560	58.716	109	43	2	25	132	29	136	1.73e-34	117
MS.gene27823.t1	AT4G13570	47.826	92	46	2	32	122	28	118	8.57e-20	79.7
MS.gene27823.t1	AT4G13570	47.253	91	46	2	33	122	35	124	2.67e-19	78.6

Find 43 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGCCTTAGAAGGAGATTT+AGG	0.266583	4.1:+13509378	None:intergenic
AAACTAATCTAAGCCTTCTT+AGG	0.296900	4.1:+13509348	None:intergenic
AAAAGAACAGGGTTAATATT+AGG	0.325842	4.1:+13509432	None:intergenic
TGCTGCTGAAGTTCTTGAAT+TGG	0.339223	4.1:-13509583	MS.gene27823:CDS
CAATTCCCAGTCGGAAGAAT+TGG	0.342053	4.1:-13509687	MS.gene27823:CDS
CTTGTTACCGACTTCTTCCT+TGG	0.365854	4.1:+13509729	None:intergenic
GGAGCCTAAATCTCCTTCTA+AGG	0.376066	4.1:-13509382	MS.gene27823:CDS
GAAAACTGAGAAAGCTGTTA+AGG	0.380382	4.1:-13509403	MS.gene27823:CDS
GAGCTTGGGAAATTGCTTAC+TGG	0.397564	4.1:-13509483	MS.gene27823:CDS
AGAACTGCGGCTAGGTAAAC+AGG	0.399219	4.1:+13509615	None:intergenic
AGCACAAAGGCGACGAAGAA+AGG	0.403145	4.1:-13509777	MS.gene27823:CDS
GCTGAAGTTCTTGAATTGGC+TGG	0.461152	4.1:-13509579	MS.gene27823:CDS
TCCGACTGGGAATTGAAGTC+CGG	0.472609	4.1:+13509695	None:intergenic
GAAGGAAAGGCGGAGGACCA+AGG	0.493860	4.1:-13509746	MS.gene27823:CDS
TATACCAAGGCATGTGTTGT+TGG	0.495755	4.1:-13509523	MS.gene27823:CDS
ATTGGCCGTTACTTGAAGAA+AGG	0.513109	4.1:-13509669	MS.gene27823:CDS
TAACGGCCAATTCTTCCGAC+TGG	0.515556	4.1:+13509681	None:intergenic
AAAGGAGCTGGAGGAAGGAA+AGG	0.517528	4.1:-13509759	MS.gene27823:CDS
CACGTGATAACAAGAAGAAT+CGG	0.520472	4.1:-13509548	MS.gene27823:CDS
CGAAGAAAGGAGCTGGAGGA+AGG	0.526335	4.1:-13509764	MS.gene27823:CDS
GCTGGAGGAAGGAAAGGCGG+AGG	0.531971	4.1:-13509753	MS.gene27823:CDS
TACGCTCAACGTGTTGGCAC+CGG	0.537157	4.1:-13509642	MS.gene27823:CDS
AAGGCGACGAAGAAAGGAGC+TGG	0.543027	4.1:-13509771	MS.gene27823:CDS
GTGAGGAATGATGAAGAGCT+TGG	0.543487	4.1:-13509498	MS.gene27823:CDS
ACTGGTGTTACCATTGCTCA+TGG	0.545887	4.1:-13509465	MS.gene27823:CDS
ACAGCCAACAACACATGCCT+TGG	0.551257	4.1:+13509519	None:intergenic
TGAGGAATGATGAAGAGCTT+GGG	0.560327	4.1:-13509497	MS.gene27823:CDS
GGTCGATACGCTCAACGTGT+TGG	0.571007	4.1:-13509648	MS.gene27823:CDS
ATCGACCTTTCTTCAAGTAA+CGG	0.579425	4.1:+13509664	None:intergenic
GGCTAGGTAAACAGGAGCAC+CGG	0.582435	4.1:+13509623	None:intergenic
GGAGCTGGAGGAAGGAAAGG+CGG	0.593373	4.1:-13509756	MS.gene27823:CDS
AACGGCCAATTCTTCCGACT+GGG	0.600319	4.1:+13509682	None:intergenic
GTAACAAGATCAATCAGAGC+CGG	0.606604	4.1:-13509714	MS.gene27823:CDS
GATATTCAAGAACTGCGGCT+AGG	0.617853	4.1:+13509607	None:intergenic
AAGAACACCACCATGAGCAA+TGG	0.624982	4.1:+13509455	None:intergenic
GCCGGACTTCAATTCCCAGT+CGG	0.639243	4.1:-13509696	MS.gene27823:CDS
CGGAGGACCAAGGAAGAAGT+CGG	0.640675	4.1:-13509736	MS.gene27823:CDS
GCGACGAAGAAAGGAGCTGG+AGG	0.642298	4.1:-13509768	MS.gene27823:CDS
GAAAATGGACGCAAGCACAA+AGG	0.663268	4.1:-13509790	None:intergenic
GGCATGTGTTGTTGGCTGTG+AGG	0.671767	4.1:-13509515	MS.gene27823:CDS
AGAAGAATCGGATTATACCA+AGG	0.672842	4.1:-13509536	MS.gene27823:CDS
AGCAAGATATTCAAGAACTG+CGG	0.685369	4.1:+13509602	None:intergenic
GGTGTTACCATTGCTCATGG+TGG	0.690637	4.1:-13509462	MS.gene27823:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAAGAACAGGGTTAATATT+AGG	+	chr4.1:13509713-13509732	None:intergenic	25.0%
!	TTTTCTTAGGCAAAAGAACA+GGG	+	chr4.1:13509724-13509743	None:intergenic	30.0%
	AGAAGAATCGGATTATACCA+AGG	-	chr4.1:13509606-13509625	MS.gene27823:CDS	35.0%
	AGCAAGATATTCAAGAACTG+CGG	+	chr4.1:13509543-13509562	None:intergenic	35.0%
	ATCGACCTTTCTTCAAGTAA+CGG	+	chr4.1:13509481-13509500	None:intergenic	35.0%
	CACGTGATAACAAGAAGAAT+CGG	-	chr4.1:13509594-13509613	MS.gene27823:CDS	35.0%
	GAAAACTGAGAAAGCTGTTA+AGG	-	chr4.1:13509739-13509758	MS.gene27823:CDS	35.0%
!	ACAGCTTTCTCAGTTTTCTT+AGG	+	chr4.1:13509737-13509756	None:intergenic	35.0%
!	GTTTTCTTAGGCAAAAGAAC+AGG	+	chr4.1:13509725-13509744	None:intergenic	35.0%
!!	TTAGCCTTAGAAGGAGATTT+AGG	+	chr4.1:13509767-13509786	None:intergenic	35.0%
	ATTGGCCGTTACTTGAAGAA+AGG	-	chr4.1:13509473-13509492	MS.gene27823:CDS	40.0%
	GGCTAAAAAGTCTCCTAAGA+AGG	-	chr4.1:13509781-13509800	MS.gene27823:CDS	40.0%
	GTAACAAGATCAATCAGAGC+CGG	-	chr4.1:13509428-13509447	MS.gene27823:CDS	40.0%
	TATACCAAGGCATGTGTTGT+TGG	-	chr4.1:13509619-13509638	MS.gene27823:CDS	40.0%
	TGAGGAATGATGAAGAGCTT+GGG	-	chr4.1:13509645-13509664	MS.gene27823:CDS	40.0%
	TGCTGCTGAAGTTCTTGAAT+TGG	-	chr4.1:13509559-13509578	MS.gene27823:CDS	40.0%
!!!	GGAGACTTTTTAGCCTTAGA+AGG	+	chr4.1:13509776-13509795	None:intergenic	40.0%
	AAGAACACCACCATGAGCAA+TGG	+	chr4.1:13509690-13509709	None:intergenic	45.0%
	CAATTCCCAGTCGGAAGAAT+TGG	-	chr4.1:13509455-13509474	MS.gene27823:CDS	45.0%
	GAGCTTGGGAAATTGCTTAC+TGG	-	chr4.1:13509659-13509678	MS.gene27823:CDS	45.0%
	GATATTCAAGAACTGCGGCT+AGG	+	chr4.1:13509538-13509557	None:intergenic	45.0%
	GCTGAAGTTCTTGAATTGGC+TGG	-	chr4.1:13509563-13509582	MS.gene27823:CDS	45.0%
	GGAGCCTAAATCTCCTTCTA+AGG	-	chr4.1:13509760-13509779	MS.gene27823:CDS	45.0%
	GTGAGGAATGATGAAGAGCT+TGG	-	chr4.1:13509644-13509663	MS.gene27823:CDS	45.0%
!!	ACTGGTGTTACCATTGCTCA+TGG	-	chr4.1:13509677-13509696	MS.gene27823:CDS	45.0%
!!	CTTGTTACCGACTTCTTCCT+TGG	+	chr4.1:13509416-13509435	None:intergenic	45.0%
	AAAGGAGCTGGAGGAAGGAA+AGG	-	chr4.1:13509383-13509402	MS.gene27823:CDS	50.0%
	AACGGCCAATTCTTCCGACT+GGG	+	chr4.1:13509463-13509482	None:intergenic	50.0%
	ACAGCCAACAACACATGCCT+TGG	+	chr4.1:13509626-13509645	None:intergenic	50.0%
	AGAACTGCGGCTAGGTAAAC+AGG	+	chr4.1:13509530-13509549	None:intergenic	50.0%
	AGCACAAAGGCGACGAAGAA+AGG	-	chr4.1:13509365-13509384	MS.gene27823:CDS	50.0%
	TAACGGCCAATTCTTCCGAC+TGG	+	chr4.1:13509464-13509483	None:intergenic	50.0%
	TCCGACTGGGAATTGAAGTC+CGG	+	chr4.1:13509450-13509469	None:intergenic	50.0%
!	GGTGTTACCATTGCTCATGG+TGG	-	chr4.1:13509680-13509699	MS.gene27823:CDS	50.0%
	AAGGCGACGAAGAAAGGAGC+TGG	-	chr4.1:13509371-13509390	MS.gene27823:CDS	55.0%
	CGAAGAAAGGAGCTGGAGGA+AGG	-	chr4.1:13509378-13509397	MS.gene27823:CDS	55.0%
	CGGAGGACCAAGGAAGAAGT+CGG	-	chr4.1:13509406-13509425	MS.gene27823:CDS	55.0%
	GCCGGACTTCAATTCCCAGT+CGG	-	chr4.1:13509446-13509465	MS.gene27823:CDS	55.0%
	GGTCGATACGCTCAACGTGT+TGG	-	chr4.1:13509494-13509513	MS.gene27823:CDS	55.0%
!	GGCATGTGTTGTTGGCTGTG+AGG	-	chr4.1:13509627-13509646	MS.gene27823:CDS	55.0%
!	GGCTAGGTAAACAGGAGCAC+CGG	+	chr4.1:13509522-13509541	None:intergenic	55.0%
!	TACGCTCAACGTGTTGGCAC+CGG	-	chr4.1:13509500-13509519	MS.gene27823:CDS	55.0%
	GAAGGAAAGGCGGAGGACCA+AGG	-	chr4.1:13509396-13509415	MS.gene27823:CDS	60.0%
	GCGACGAAGAAAGGAGCTGG+AGG	-	chr4.1:13509374-13509393	MS.gene27823:CDS	60.0%
	GGAGCTGGAGGAAGGAAAGG+CGG	-	chr4.1:13509386-13509405	MS.gene27823:CDS	60.0%
	GCTGGAGGAAGGAAAGGCGG+AGG	-	chr4.1:13509389-13509408	MS.gene27823:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	13509356	13509808	13509356	ID=MS.gene27823
chr4.1	mRNA	13509356	13509808	13509356	ID=MS.gene27823.t1;Parent=MS.gene27823
chr4.1	exon	13509356	13509808	13509356	ID=MS.gene27823.t1.exon1;Parent=MS.gene27823.t1
chr4.1	CDS	13509356	13509808	13509356	ID=cds.MS.gene27823.t1;Parent=MS.gene27823.t1

Gene Sequence

>MS.gene27823

ATGGACGCAAGCACAAAGGCGACGAAGAAAGGAGCTGGAGGAAGGAAAGGCGGAGGACCAAGGAAGAAGTCGGTAACAAGATCAATCAGAGCCGGACTTCAATTCCCAGTCGGAAGAATTGGCCGTTACTTGAAGAAAGGTCGATACGCTCAACGTGTTGGCACCGGTGCTCCTGTTTACCTAGCCGCAGTTCTTGAATATCTTGCTGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATCGGATTATACCAAGGCATGTGTTGTTGGCTGTGAGGAATGATGAAGAGCTTGGGAAATTGCTTACTGGTGTTACCATTGCTCATGGTGGTGTTCTTCCTAATATTAACCCTGTTCTTTTGCCTAAGAAAACTGAGAAAGCTGTTAAGGAGCCTAAATCTCCTTCTAAGGCTAAAAAGTCTCCTAAGAAGGCTTAG

Protein sequence

>MS.gene27823.t1

MDASTKATKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLTGVTIAHGGVLPNINPVLLPKKTEKAVKEPKSPSKAKKSPKKA