AlfalfaGEDB | MS.gene30863

M. sativa cultivar XinJiangDaYe / MS.gene30863

KEGG: K02918
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00831.26

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30863.t1	XP_004494985.1	97	99	3	0	1	99	1	99	1.50E-40	175.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30863.t1	Q9M5L0	91.9	99	8	0	1	99	1	99	4.8e-40	164.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30863.t1	A0A1S2XV31	97.0	99	3	0	1	99	1	99	1.1e-40	175.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049340	MS.gene30863	0.807396	5.16E-50	-1.69E-46
MS.gene049341	MS.gene30863	0.807215	5.64E-50	-1.69E-46
MS.gene049825	MS.gene30863	0.815443	9.22E-52	-1.69E-46
MS.gene049873	MS.gene30863	0.805293	1.43E-49	-1.69E-46
MS.gene050277	MS.gene30863	0.844159	8.98E-59	-1.69E-46
MS.gene050303	MS.gene30863	0.803289	3.74E-49	-1.69E-46
MS.gene050916	MS.gene30863	0.811877	5.62E-51	-1.69E-46
MS.gene051063	MS.gene30863	0.814684	1.36E-51	-1.69E-46
MS.gene051330	MS.gene30863	0.823337	1.46E-53	-1.69E-46
MS.gene051746	MS.gene30863	0.805229	1.48E-49	-1.69E-46
MS.gene052879	MS.gene30863	0.824669	7.12E-54	-1.69E-46
MS.gene052880	MS.gene30863	0.810385	1.18E-50	-1.69E-46
MS.gene055580	MS.gene30863	-0.811279	7.58E-51	-1.69E-46
MS.gene055808	MS.gene30863	0.830855	2.32E-55	-1.69E-46
MS.gene056043	MS.gene30863	-0.808138	3.59E-50	-1.69E-46
MS.gene056075	MS.gene30863	0.81738	3.40E-52	-1.69E-46
MS.gene056091	MS.gene30863	0.834568	2.78E-56	-1.69E-46
MS.gene056432	MS.gene30863	0.801194	1.01E-48	-1.69E-46
MS.gene056631	MS.gene30863	0.858619	7.28E-63	-1.69E-46
MS.gene058906	MS.gene30863	0.855342	6.73E-62	-1.69E-46
MS.gene05954	MS.gene30863	0.801799	7.59E-49	-1.69E-46
MS.gene06001	MS.gene30863	0.884937	1.25E-71	-1.69E-46
MS.gene060165	MS.gene30863	0.808377	3.19E-50	-1.69E-46
MS.gene060429	MS.gene30863	0.837232	5.87E-57	-1.69E-46
MS.gene060619	MS.gene30863	0.813429	2.57E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30863.t1	MTR_1g067490	95.833	96	4	0	4	99	3	98	1.89e-59	178
MS.gene30863.t1	MTR_4g062410	88.889	99	11	0	1	99	1	99	1.61e-56	171
MS.gene30863.t1	MTR_1g115545	92.708	96	7	0	4	99	2	97	2.05e-56	170
MS.gene30863.t1	MTR_7g080090	86.869	99	13	0	1	99	1	99	6.84e-54	164
MS.gene30863.t1	MTR_4g072410	85.417	96	14	0	5	100	3	98	2.56e-51	157
MS.gene30863.t1	MTR_1g105880	73.256	86	12	1	14	99	5	79	1.73e-34	114
MS.gene30863.t1	MTR_5g045540	87.879	33	4	0	67	99	2	34	4.02e-13	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30863.t1	AT5G02610	87.879	99	12	0	1	99	1	99	6.85e-55	166
MS.gene30863.t1	AT2G39390	87.000	100	13	0	1	100	1	100	1.61e-54	166
MS.gene30863.t1	AT3G09500	87.000	100	13	0	1	100	1	100	1.68e-54	166
MS.gene30863.t1	AT3G55170	86.000	100	14	0	1	100	1	100	7.03e-54	163
MS.gene30863.t1	AT3G55170	86.000	100	14	0	1	100	1	100	7.03e-54	163
MS.gene30863.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene30863.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene30863.t1	AT3G55170	85.000	100	15	0	1	100	1	100	6.85e-53	161
MS.gene30863.t1	AT5G02610	71.311	122	12	1	1	99	1	122	4.42e-49	152

Find 19 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCATTTGAAGTGAGAATTA+AGG	0.291537	1.1:+49688190	MS.gene30863:intron
CTGATAGCACGGGTCTTCTT+AGG	0.307833	1.1:-49688539	None:intergenic
CTGGTTCAGCAAATCAGCTT+TGG	0.322482	1.1:-49688234	None:intergenic
TGGTTCAGCAAATCAGCTTT+GGG	0.386643	1.1:-49688233	None:intergenic
AGGTGCACCGCCGGTGACTT+TGG	0.448506	1.1:-49688297	None:intergenic
ATTAAACCTGGTGTTTGGTA+AGG	0.478429	1.1:-49688568	None:intergenic
CCGCCTTCAGATCCTTCAGC+TGG	0.492136	1.1:-49688253	None:intergenic
CTTCGAGTCGCCAAAGTCAC+CGG	0.499663	1.1:+49688287	MS.gene30863:CDS
GAGCTTGTTAGGTGCACCGC+CGG	0.541755	1.1:-49688306	None:intergenic
GAATTAAGGTGTTCGAGTTG+AGG	0.547821	1.1:+49688204	MS.gene30863:CDS
TGATTTGCTGAACCAGCTGA+AGG	0.570604	1.1:+49688241	MS.gene30863:CDS
GAACCAGCTGAAGGATCTGA+AGG	0.580944	1.1:+49688250	MS.gene30863:CDS
TAAGGCGTCTTCTGATAGCA+CGG	0.582904	1.1:-49688550	None:intergenic
TTCTTAGGACGAAGATCAAG+TGG	0.602701	1.1:-49688524	None:intergenic
AAGGCGTCTTCTGATAGCAC+GGG	0.605672	1.1:-49688549	None:intergenic
AAGACGCCTTACCAAACACC+AGG	0.624051	1.1:+49688562	MS.gene30863:CDS
CGAGTCGCCAAAGTCACCGG+CGG	0.675842	1.1:+49688290	MS.gene30863:CDS
CCAGCTGAAGGATCTGAAGG+CGG	0.695519	1.1:+49688253	MS.gene30863:CDS
GCAGAAAGCTGCGCTCAGAG+AGG	0.696591	1.1:+49688478	MS.gene30863:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTATGTATTGTTTACATTT+TGG	-	chr1.1:49688333-49688352	None:intergenic	15.0%
!!!	AATACATAATCGCTTTTATA+TGG	+	chr1.1:49688343-49688362	MS.gene30863:intron	20.0%
	AATTCTCACTTCAAATGCAA+TGG	-	chr1.1:49688189-49688208	None:intergenic	30.0%
	TTATATGGAAGCTGCTTATA+TGG	+	chr1.1:49688358-49688377	MS.gene30863:intron	30.0%
!	TGCATTTGAAGTGAGAATTA+AGG	+	chr1.1:49688190-49688209	MS.gene30863:intron	30.0%
!!!	TTTTTTACAGCAAAGTTGTG+AGG	+	chr1.1:49688417-49688436	MS.gene30863:intron	30.0%
!!	TACATTTTGGAGAGCTTGTT+AGG	-	chr1.1:49688320-49688339	None:intergenic	35.0%
!!	TGGTAATCGCGTTTTGTTAA+TGG	+	chr1.1:49688378-49688397	MS.gene30863:intron	35.0%
	TGGTTCAGCAAATCAGCTTT+GGG	-	chr1.1:49688236-49688255	None:intergenic	40.0%
	TTCTTAGGACGAAGATCAAG+TGG	-	chr1.1:49688527-49688546	None:intergenic	40.0%
!!	GAATTAAGGTGTTCGAGTTG+AGG	+	chr1.1:49688204-49688223	MS.gene30863:CDS	40.0%
	CTGGTTCAGCAAATCAGCTT+TGG	-	chr1.1:49688237-49688256	None:intergenic	45.0%
!	TAAGGCGTCTTCTGATAGCA+CGG	-	chr1.1:49688553-49688572	None:intergenic	45.0%
!!	TGATTTGCTGAACCAGCTGA+AGG	+	chr1.1:49688241-49688260	MS.gene30863:CDS	45.0%
	AAGACGCCTTACCAAACACC+AGG	+	chr1.1:49688562-49688581	MS.gene30863:CDS	50.0%
	CTGATAGCACGGGTCTTCTT+AGG	-	chr1.1:49688542-49688561	None:intergenic	50.0%
	GAACCAGCTGAAGGATCTGA+AGG	+	chr1.1:49688250-49688269	MS.gene30863:CDS	50.0%
!	AAGGCGTCTTCTGATAGCAC+GGG	-	chr1.1:49688552-49688571	None:intergenic	50.0%
	CCAGCTGAAGGATCTGAAGG+CGG	+	chr1.1:49688253-49688272	MS.gene30863:CDS	55.0%
	CTTCGAGTCGCCAAAGTCAC+CGG	+	chr1.1:49688287-49688306	MS.gene30863:CDS	55.0%
	CCGCCTTCAGATCCTTCAGC+TGG	-	chr1.1:49688256-49688275	None:intergenic	60.0%
	GCAGAAAGCTGCGCTCAGAG+AGG	+	chr1.1:49688478-49688497	MS.gene30863:CDS	60.0%
!!	GAGCTTGTTAGGTGCACCGC+CGG	-	chr1.1:49688309-49688328	None:intergenic	60.0%
	AGGTGCACCGCCGGTGACTT+TGG	-	chr1.1:49688300-49688319	None:intergenic	65.0%
	CGAGTCGCCAAAGTCACCGG+CGG	+	chr1.1:49688290-49688309	MS.gene30863:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	49688099	49688589	49688099	ID=MS.gene30863
chr1.1	mRNA	49688099	49688589	49688099	ID=MS.gene30863.t1;Parent=MS.gene30863
chr1.1	exon	49688099	49688102	49688099	ID=MS.gene30863.t1.exon1;Parent=MS.gene30863.t1
chr1.1	CDS	49688099	49688102	49688099	ID=cds.MS.gene30863.t1;Parent=MS.gene30863.t1
chr1.1	exon	49688201	49688336	49688201	ID=MS.gene30863.t1.exon2;Parent=MS.gene30863.t1
chr1.1	CDS	49688201	49688336	49688201	ID=cds.MS.gene30863.t1;Parent=MS.gene30863.t1
chr1.1	exon	49688427	49688589	49688427	ID=MS.gene30863.t1.exon3;Parent=MS.gene30863.t1
chr1.1	CDS	49688427	49688589	49688427	ID=cds.MS.gene30863.t1;Parent=MS.gene30863.t1

Gene Sequence

>MS.gene30863

ATGGGTAAGAATGTGAATTTAGTTTCAGTAACGATTTAATCAAATCAAATTTGTAACATTTTTCAACATATAAGCTGAATTGAATTTCCATTGCATTTGAAGTGAGAATTAAGGTGTTCGAGTTGAGGAACAAACCCAAAGCTGATTTGCTGAACCAGCTGAAGGATCTGAAGGCGGAGCTCGCTTTGCTTCGAGTCGCCAAAGTCACCGGCGGTGCACCTAACAAGCTCTCCAAAATGTAAACAATACATAATCGCTTTTATATGGAAGCTGCTTATATGGTAATCGCGTTTTGTTAATGGTGTTTTTGTTTGTTTGTTTTTTACAGCAAAGTTGTGAGGCTTTCAATTGCACAAGTGTTGACAGTGATTTCACAGAAGCAGAAAGCTGCGCTCAGAGAGGTTTACAAAAATAAGAAGTATTTACCACTTGATCTTCGTCCTAAGAAGACCCGTGCTATCAGAAGACGCCTTACCAAACACCAGGTTTAA

Protein sequence

>MS.gene30863.t1

MVRIKVFELRNKPKADLLNQLKDLKAELALLRVAKVTGGAPNKLSKIKVVRLSIAQVLTVISQKQKAALREVYKNKKYLPLDLRPKKTRAIRRRLTKHQV