Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32866.t1 | XP_024635917.1 | 96.2 | 158 | 5 | 1 | 1 | 157 | 1 | 158 | 1.30E-75 | 292.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32866.t1 | A0A072V3C0 | 96.2 | 158 | 5 | 1 | 1 | 157 | 1 | 158 | 9.3e-76 | 292.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene32866 | 0.810306 | 1.23E-50 | -1.69E-46 |
| MS.gene04919 | MS.gene32866 | 0.819384 | 1.19E-52 | -1.69E-46 |
| MS.gene04920 | MS.gene32866 | 0.833996 | 3.87E-56 | -1.69E-46 |
| MS.gene049283 | MS.gene32866 | 0.831243 | 1.86E-55 | -1.69E-46 |
| MS.gene049499 | MS.gene32866 | 0.830865 | 2.31E-55 | -1.69E-46 |
| MS.gene050414 | MS.gene32866 | 0.801855 | 7.39E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene32866 | 0.802463 | 5.54E-49 | -1.69E-46 |
| MS.gene052296 | MS.gene32866 | 0.815601 | 8.51E-52 | -1.69E-46 |
| MS.gene052533 | MS.gene32866 | 0.81984 | 9.40E-53 | -1.69E-46 |
| MS.gene052793 | MS.gene32866 | 0.813788 | 2.14E-51 | -1.69E-46 |
| MS.gene053045 | MS.gene32866 | 0.805223 | 1.48E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene32866 | 0.800107 | 1.68E-48 | -1.69E-46 |
| MS.gene054222 | MS.gene32866 | 0.814675 | 1.37E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene32866 | 0.814203 | 1.74E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene32866 | 0.837334 | 5.53E-57 | -1.69E-46 |
| MS.gene055841 | MS.gene32866 | 0.824042 | 1.00E-53 | -1.69E-46 |
| MS.gene057102 | MS.gene32866 | 0.825351 | 4.91E-54 | -1.69E-46 |
| MS.gene057155 | MS.gene32866 | 0.811041 | 8.54E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene32866 | 0.810779 | 9.73E-51 | -1.69E-46 |
| MS.gene058159 | MS.gene32866 | 0.820383 | 7.06E-53 | -1.69E-46 |
| MS.gene058592 | MS.gene32866 | 0.818788 | 1.63E-52 | -1.69E-46 |
| MS.gene05878 | MS.gene32866 | 0.807721 | 4.40E-50 | -1.69E-46 |
| MS.gene05952 | MS.gene32866 | 0.84655 | 2.02E-59 | -1.69E-46 |
| MS.gene05985 | MS.gene32866 | -0.824686 | 7.05E-54 | -1.69E-46 |
| MS.gene06018 | MS.gene32866 | 0.805803 | 1.12E-49 | -1.69E-46 |
| MS.gene060244 | MS.gene32866 | 0.80682 | 6.83E-50 | -1.69E-46 |
| MS.gene060935 | MS.gene32866 | 0.802827 | 4.66E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene32866 | 0.809954 | 1.47E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene32866 | 0.803291 | 3.74E-49 | -1.69E-46 |
| MS.gene061243 | MS.gene32866 | 0.81535 | 9.67E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32866.t1 | MTR_3g103290 | 96.203 | 158 | 5 | 1 | 1 | 157 | 1 | 158 | 2.22e-107 | 303 |
| MS.gene32866.t1 | MTR_1g017830 | 60.625 | 160 | 54 | 6 | 6 | 157 | 4 | 162 | 3.74e-49 | 155 |
| MS.gene32866.t1 | MTR_4g097260 | 34.810 | 158 | 77 | 8 | 7 | 154 | 6 | 147 | 4.12e-14 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene32866.t1 | AT1G10140 | 42.353 | 170 | 75 | 6 | 7 | 157 | 2 | 167 | 4.29e-23 | 89.7 |
| MS.gene32866.t1 | AT1G58420 | 37.931 | 174 | 76 | 6 | 8 | 153 | 6 | 175 | 9.50e-21 | 84.0 |
Find 40 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAGCATAGCTTCTACTTCT+TGG | 0.254156 | 3.1:+81783999 | None:intergenic |
| CATACCAACACTAACGGATT+TGG | 0.266128 | 3.1:+81784094 | None:intergenic |
| TTTGGGAAACCGAATTGATA+TGG | 0.303968 | 3.1:-81784171 | MS.gene32866:CDS |
| AAACCGAATTGATATGGATT+TGG | 0.312780 | 3.1:-81784165 | MS.gene32866:CDS |
| GCAGCCTCAGCTAGGACTTT+GGG | 0.367265 | 3.1:-81784188 | MS.gene32866:CDS |
| GGAAAAGCCTAGTGATGATT+TGG | 0.370683 | 3.1:-81784057 | MS.gene32866:CDS |
| AGCAGCCTCAGCTAGGACTT+TGG | 0.381286 | 3.1:-81784189 | MS.gene32866:CDS |
| ATACCAACACTAACGGATTT+GGG | 0.389033 | 3.1:+81784095 | None:intergenic |
| CAAATCCGTTAGTGTTGGTA+TGG | 0.422959 | 3.1:-81784093 | MS.gene32866:CDS |
| GGGTAACCCGTTTGAAGAAA+CGG | 0.435006 | 3.1:+81784115 | None:intergenic |
| CAAAACCCGTTTCTTCAAAC+GGG | 0.435681 | 3.1:-81784121 | MS.gene32866:CDS |
| GAACCAAATCCATATCAATT+CGG | 0.436672 | 3.1:+81784162 | None:intergenic |
| GGTAACCCGTTTGAAGAAAC+GGG | 0.437522 | 3.1:+81784116 | None:intergenic |
| ACAAAACCCGTTTCTTCAAA+CGG | 0.444703 | 3.1:-81784122 | MS.gene32866:CDS |
| ACCTTGGAGATGAGGAAAAG+TGG | 0.460082 | 3.1:-81784434 | None:intergenic |
| GGATAAGAATTAGCCACAAC+AGG | 0.466220 | 3.1:+81784020 | None:intergenic |
| ATGAATTACAGCAATCCTAC+TGG | 0.466774 | 3.1:-81784302 | MS.gene32866:CDS |
| ACAACACCAATAAGAGTGTT+GGG | 0.479331 | 3.1:-81784374 | MS.gene32866:CDS |
| AGCGCGGTAACTTCTCGATC+TGG | 0.501278 | 3.1:-81784251 | MS.gene32866:CDS |
| AACAACACCAATAAGAGTGT+TGG | 0.503361 | 3.1:-81784375 | MS.gene32866:CDS |
| CCGTTAGTGTTGGTATGGGT+CGG | 0.519449 | 3.1:-81784088 | MS.gene32866:CDS |
| GAAAAGCCTAGTGATGATTT+GGG | 0.526662 | 3.1:-81784056 | MS.gene32866:CDS |
| GTAACTTCTCGATCTGGTGG+TGG | 0.540577 | 3.1:-81784245 | MS.gene32866:CDS |
| AGAAGTAGAAGCTATGCTGT+TGG | 0.550220 | 3.1:-81783996 | MS.gene32866:CDS |
| ACTTCTCGATCTGGTGGTGG+TGG | 0.560692 | 3.1:-81784242 | MS.gene32866:CDS |
| CCTTCACCCAAATCATCACT+AGG | 0.561979 | 3.1:+81784050 | None:intergenic |
| TAATGAGAGCAGCCTCAGCT+AGG | 0.566610 | 3.1:-81784196 | MS.gene32866:CDS |
| CCTTGGAGATGAGGAAAAGT+GGG | 0.569313 | 3.1:-81784433 | None:intergenic |
| TGAAGGTGATGTTCCTGTTG+TGG | 0.582435 | 3.1:-81784033 | MS.gene32866:CDS |
| TTACCCAAATCCGTTAGTGT+TGG | 0.593589 | 3.1:-81784098 | MS.gene32866:CDS |
| GTTACCGCGCTGTAACTTCG+CGG | 0.602266 | 3.1:+81784263 | None:intergenic |
| AAATTCTCAAATCTTCCAGT+AGG | 0.603198 | 3.1:+81784287 | None:intergenic |
| CCGACCCATACCAACACTAA+CGG | 0.605120 | 3.1:+81784088 | None:intergenic |
| AAATCCGTTAGTGTTGGTAT+GGG | 0.605156 | 3.1:-81784092 | MS.gene32866:CDS |
| CCTAGTGATGATTTGGGTGA+AGG | 0.626517 | 3.1:-81784050 | MS.gene32866:CDS |
| GCGGTAACTTCTCGATCTGG+TGG | 0.636577 | 3.1:-81784248 | MS.gene32866:CDS |
| GTTTCCCAAAGTCCTAGCTG+AGG | 0.653181 | 3.1:+81784184 | None:intergenic |
| TGGTATGGGTCGGATTGATG+AGG | 0.659406 | 3.1:-81784078 | MS.gene32866:CDS |
| GTAAGAAGCATAACAAAGTG+CGG | 0.704624 | 3.1:-81784332 | MS.gene32866:CDS |
| TTTACCGCGAAGTTACAGCG+CGG | 0.832668 | 3.1:-81784267 | MS.gene32866:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACCGAATTGATATGGATT+TGG | - | chr3.1:81784234-81784253 | MS.gene32866:CDS | 30.0% | |
| AAATTCTCAAATCTTCCAGT+AGG | + | chr3.1:81784115-81784134 | None:intergenic | 30.0% | |
| GAACCAAATCCATATCAATT+CGG | + | chr3.1:81784240-81784259 | None:intergenic | 30.0% | |
| ACAAAACCCGTTTCTTCAAA+CGG | - | chr3.1:81784277-81784296 | MS.gene32866:CDS | 35.0% | |
| ATGAATTACAGCAATCCTAC+TGG | - | chr3.1:81784097-81784116 | MS.gene32866:CDS | 35.0% | |
| GAAAAGCCTAGTGATGATTT+GGG | - | chr3.1:81784343-81784362 | MS.gene32866:CDS | 35.0% | |
| TTTGGGAAACCGAATTGATA+TGG | - | chr3.1:81784228-81784247 | MS.gene32866:CDS | 35.0% | |
| ! | AACAACACCAATAAGAGTGT+TGG | - | chr3.1:81784024-81784043 | MS.gene32866:CDS | 35.0% |
| ! | GTAAGAAGCATAACAAAGTG+CGG | - | chr3.1:81784067-81784086 | MS.gene32866:CDS | 35.0% |
| !! | AAATCCGTTAGTGTTGGTAT+GGG | - | chr3.1:81784307-81784326 | MS.gene32866:CDS | 35.0% |
| !! | ACAACACCAATAAGAGTGTT+GGG | - | chr3.1:81784025-81784044 | MS.gene32866:CDS | 35.0% |
| !! | ATACCAACACTAACGGATTT+GGG | + | chr3.1:81784307-81784326 | None:intergenic | 35.0% |
| ACAGCATAGCTTCTACTTCT+TGG | + | chr3.1:81784403-81784422 | None:intergenic | 40.0% | |
| AGAAGTAGAAGCTATGCTGT+TGG | - | chr3.1:81784403-81784422 | MS.gene32866:CDS | 40.0% | |
| CAAAACCCGTTTCTTCAAAC+GGG | - | chr3.1:81784278-81784297 | MS.gene32866:CDS | 40.0% | |
| GGAAAAGCCTAGTGATGATT+TGG | - | chr3.1:81784342-81784361 | MS.gene32866:CDS | 40.0% | |
| GGATAAGAATTAGCCACAAC+AGG | + | chr3.1:81784382-81784401 | None:intergenic | 40.0% | |
| TTACCCAAATCCGTTAGTGT+TGG | - | chr3.1:81784301-81784320 | MS.gene32866:CDS | 40.0% | |
| ! | GCTTTTCCCAACACTCTTAT+TGG | + | chr3.1:81784034-81784053 | None:intergenic | 40.0% |
| !! | CAAATCCGTTAGTGTTGGTA+TGG | - | chr3.1:81784306-81784325 | MS.gene32866:CDS | 40.0% |
| !! | CATACCAACACTAACGGATT+TGG | + | chr3.1:81784308-81784327 | None:intergenic | 40.0% |
| CCTTCACCCAAATCATCACT+AGG | + | chr3.1:81784352-81784371 | None:intergenic | 45.0% | |
| GGGTAACCCGTTTGAAGAAA+CGG | + | chr3.1:81784287-81784306 | None:intergenic | 45.0% | |
| GGTAACCCGTTTGAAGAAAC+GGG | + | chr3.1:81784286-81784305 | None:intergenic | 45.0% | |
| TGAAGGTGATGTTCCTGTTG+TGG | - | chr3.1:81784366-81784385 | MS.gene32866:CDS | 45.0% | |
| ! | CCTAGTGATGATTTGGGTGA+AGG | - | chr3.1:81784349-81784368 | MS.gene32866:CDS | 45.0% |
| CCGACCCATACCAACACTAA+CGG | + | chr3.1:81784314-81784333 | None:intergenic | 50.0% | |
| GTAACTTCTCGATCTGGTGG+TGG | - | chr3.1:81784154-81784173 | MS.gene32866:CDS | 50.0% | |
| GTTTCCCAAAGTCCTAGCTG+AGG | + | chr3.1:81784218-81784237 | None:intergenic | 50.0% | |
| TAATGAGAGCAGCCTCAGCT+AGG | - | chr3.1:81784203-81784222 | MS.gene32866:CDS | 50.0% | |
| TGGTATGGGTCGGATTGATG+AGG | - | chr3.1:81784321-81784340 | MS.gene32866:CDS | 50.0% | |
| TTTACCGCGAAGTTACAGCG+CGG | - | chr3.1:81784132-81784151 | MS.gene32866:CDS | 50.0% | |
| !! | CCGTTAGTGTTGGTATGGGT+CGG | - | chr3.1:81784311-81784330 | MS.gene32866:CDS | 50.0% |
| AGCAGCCTCAGCTAGGACTT+TGG | - | chr3.1:81784210-81784229 | MS.gene32866:CDS | 55.0% | |
| AGCGCGGTAACTTCTCGATC+TGG | - | chr3.1:81784148-81784167 | MS.gene32866:CDS | 55.0% | |
| GCAGCCTCAGCTAGGACTTT+GGG | - | chr3.1:81784211-81784230 | MS.gene32866:CDS | 55.0% | |
| GCGGTAACTTCTCGATCTGG+TGG | - | chr3.1:81784151-81784170 | MS.gene32866:CDS | 55.0% | |
| GTTACCGCGCTGTAACTTCG+CGG | + | chr3.1:81784139-81784158 | None:intergenic | 55.0% | |
| !! | ACTTCTCGATCTGGTGGTGG+TGG | - | chr3.1:81784157-81784176 | MS.gene32866:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 81783974 | 81784447 | 81783974 | ID=MS.gene32866 |
| chr3.1 | mRNA | 81783974 | 81784447 | 81783974 | ID=MS.gene32866.t1;Parent=MS.gene32866 |
| chr3.1 | exon | 81783974 | 81784447 | 81783974 | ID=MS.gene32866.t1.exon1;Parent=MS.gene32866.t1 |
| chr3.1 | CDS | 81783974 | 81784447 | 81783974 | ID=cds.MS.gene32866.t1;Parent=MS.gene32866.t1 |
| Gene Sequence |
| Protein sequence |
