AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene061243


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061243.t1 MTR_4g122800 96.703 182 6 0 1 182 1 182 6.69e-132 367
MS.gene061243.t1 MTR_4g122750 49.451 182 83 2 1 182 1 173 9.13e-56 174
MS.gene061243.t1 MTR_4g122890 44.262 183 93 3 1 182 1 175 1.64e-47 153
MS.gene061243.t1 MTR_4g122760 44.086 186 90 3 1 182 1 176 9.00e-46 149
MS.gene061243.t1 MTR_4g007280 50.000 108 52 2 77 182 1 108 2.62e-26 97.1
MS.gene061243.t1 MTR_4g122780 37.086 151 79 4 48 182 8 158 1.69e-19 81.3
MS.gene061243.t1 MTR_2g033630 30.857 175 108 3 12 182 11 176 7.28e-19 79.7
MS.gene061243.t1 MTR_4g122660 27.624 181 116 6 9 182 4 176 7.19e-18 77.4
MS.gene061243.t1 MTR_4g122740 31.609 174 110 4 12 182 10 177 2.47e-16 73.2
MS.gene061243.t1 MTR_4g122720 28.082 146 101 2 41 182 40 185 3.61e-13 65.1
MS.gene061243.t1 MTR_2g033740 30.114 176 98 6 22 181 20 186 1.62e-12 63.2
MS.gene061243.t1 MTR_2g033720 29.480 173 103 5 22 181 20 186 1.79e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene061243.t1 AT1G47960 46.154 143 77 0 40 182 24 166 4.50e-40 134
MS.gene061243.t1 AT1G47960 44.928 138 76 0 40 177 24 161 1.58e-36 126
MS.gene061243.t1 AT3G17140 36.842 95 58 2 89 182 19 112 1.25e-13 64.3
MS.gene061243.t1 AT3G17150 27.273 143 102 1 42 182 39 181 9.84e-12 61.2
MS.gene061243.t1 AT3G17152 26.404 178 125 4 7 181 1 175 1.15e-11 60.8

Find 34 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGGCAAACATTGTAATT+TGG 0.270319 8.3:-6002091 None:intergenic
CAGTCTTTGAAGGCATATTC+TGG 0.284723 8.3:+6002112 MS.gene061243:CDS
ACCTCTGCTGGTGATGTAAC+AGG 0.308788 8.3:+6002136 MS.gene061243:CDS
GTACTTCTGCTTTGGATTGT+TGG 0.314380 8.3:-6002010 None:intergenic
GGAAATTAGTACTTCTGCTT+TGG 0.326142 8.3:-6002018 None:intergenic
ACCTCAAGCTATTGAAGCTT+TGG 0.326895 8.3:+6002306 MS.gene061243:CDS
AGGAATAAGCAAAGAGTTAG+AGG 0.339032 8.3:-6001959 None:intergenic
AGCTACAAAGGTTGAGAAAT+GGG 0.347926 8.3:+6002224 MS.gene061243:CDS
CACCATCTTCTGCAAACTTA+GGG 0.376121 8.3:-6002339 None:intergenic
GCACCATCTTCTGCAAACTT+AGG 0.382996 8.3:-6002340 None:intergenic
TTCTCAACCTTTGTAGCTGG+TGG 0.401916 8.3:-6002219 None:intergenic
GCTATTGAAGCTTTGGAGAA+AGG 0.407146 8.3:+6002313 MS.gene061243:CDS
CATTTCTCAACCTTTGTAGC+TGG 0.408972 8.3:-6002222 None:intergenic
CTATTGAAGCTTTGGAGAAA+GGG 0.422212 8.3:+6002314 MS.gene061243:CDS
TGTTTGCCTTCAGTCTTTGA+AGG 0.426146 8.3:+6002102 MS.gene061243:CDS
CAGCTACAAAGGTTGAGAAA+TGG 0.460710 8.3:+6002223 MS.gene061243:CDS
ATTGTGAGAGTGAGTTCAAT+GGG 0.471484 8.3:+6002389 MS.gene061243:CDS
TTACGATAACAATAGCTTGA+AGG 0.481941 8.3:-6001979 None:intergenic
GCATATTCTGGTACCTCTGC+TGG 0.484173 8.3:+6002124 MS.gene061243:CDS
AAGTACTAATTTCCCTTCAA+AGG 0.496589 8.3:+6002027 MS.gene061243:CDS
GACCCTAAGTTTGCAGAAGA+TGG 0.509313 8.3:+6002337 MS.gene061243:CDS
TATTGTGAGAGTGAGTTCAA+TGG 0.517229 8.3:+6002388 MS.gene061243:CDS
AGGAAAGCTTTGAGTTCTTG+TGG 0.525393 8.3:+6002250 MS.gene061243:CDS
TCCAAAGCTTCAATAGCTTG+AGG 0.539762 8.3:-6002307 None:intergenic
AAAGGTTGAGAAATGGGGCT+AGG 0.543045 8.3:+6002230 MS.gene061243:CDS
TGCAAATGATGCTGCTAATG+AGG 0.560849 8.3:+6002360 MS.gene061243:CDS
AACAGGGCTAGCACAAATCA+TGG 0.565524 8.3:+6002153 MS.gene061243:CDS
ATAGAATCCACCAGCTACAA+AGG 0.567634 8.3:+6002212 MS.gene061243:CDS
TATTGAAGCTTTGGAGAAAG+GGG 0.591796 8.3:+6002315 MS.gene061243:CDS
CCCTGTTACATCACCAGCAG+AGG 0.602085 8.3:-6002137 None:intergenic
CCTCTGCTGGTGATGTAACA+GGG 0.604777 8.3:+6002137 MS.gene061243:CDS
AATATGCCTTCAAAGACTGA+AGG 0.626365 8.3:-6002108 None:intergenic
ATGAAAGCAAAAGCAAACGA+TGG 0.653893 8.3:+6002184 MS.gene061243:CDS
GCTACAAAGGTTGAGAAATG+GGG 0.661587 8.3:+6002225 MS.gene061243:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGTTTTTCATCCTTTGAA+GGG - chr8.3:6002042-6002061 None:intergenic 25.0%
AAGTACTAATTTCCCTTCAA+AGG + chr8.3:6002027-6002046 MS.gene061243:CDS 30.0%
TGAAGGCAAACATTGTAATT+TGG - chr8.3:6002094-6002113 None:intergenic 30.0%
TTACGATAACAATAGCTTGA+AGG - chr8.3:6001982-6002001 None:intergenic 30.0%
! GATAGTTTTTCATCCTTTGA+AGG - chr8.3:6002043-6002062 None:intergenic 30.0%
AATATGCCTTCAAAGACTGA+AGG - chr8.3:6002111-6002130 None:intergenic 35.0%
AGCTACAAAGGTTGAGAAAT+GGG + chr8.3:6002224-6002243 MS.gene061243:CDS 35.0%
AGGAATAAGCAAAGAGTTAG+AGG - chr8.3:6001962-6001981 None:intergenic 35.0%
ATGAAAGCAAAAGCAAACGA+TGG + chr8.3:6002184-6002203 MS.gene061243:CDS 35.0%
ATTGTGAGAGTGAGTTCAAT+GGG + chr8.3:6002389-6002408 MS.gene061243:CDS 35.0%
GGAAATTAGTACTTCTGCTT+TGG - chr8.3:6002021-6002040 None:intergenic 35.0%
TATTGTGAGAGTGAGTTCAA+TGG + chr8.3:6002388-6002407 MS.gene061243:CDS 35.0%
!! CTATTGAAGCTTTGGAGAAA+GGG + chr8.3:6002314-6002333 MS.gene061243:CDS 35.0%
!! TATTGAAGCTTTGGAGAAAG+GGG + chr8.3:6002315-6002334 MS.gene061243:CDS 35.0%
ACCTCAAGCTATTGAAGCTT+TGG + chr8.3:6002306-6002325 MS.gene061243:CDS 40.0%
ATAGAATCCACCAGCTACAA+AGG + chr8.3:6002212-6002231 MS.gene061243:CDS 40.0%
CACCATCTTCTGCAAACTTA+GGG - chr8.3:6002342-6002361 None:intergenic 40.0%
CAGCTACAAAGGTTGAGAAA+TGG + chr8.3:6002223-6002242 MS.gene061243:CDS 40.0%
CATTTCTCAACCTTTGTAGC+TGG - chr8.3:6002225-6002244 None:intergenic 40.0%
GCTACAAAGGTTGAGAAATG+GGG + chr8.3:6002225-6002244 MS.gene061243:CDS 40.0%
TCCAAAGCTTCAATAGCTTG+AGG - chr8.3:6002310-6002329 None:intergenic 40.0%
TGCAAATGATGCTGCTAATG+AGG + chr8.3:6002360-6002379 MS.gene061243:CDS 40.0%
TGTTTGCCTTCAGTCTTTGA+AGG + chr8.3:6002102-6002121 MS.gene061243:CDS 40.0%
! CAGTCTTTGAAGGCATATTC+TGG + chr8.3:6002112-6002131 MS.gene061243:CDS 40.0%
! GCATTGTTCTGTTTTGTGAG+TGG - chr8.3:6002421-6002440 None:intergenic 40.0%
! GTACTTCTGCTTTGGATTGT+TGG - chr8.3:6002013-6002032 None:intergenic 40.0%
!! AGGAAAGCTTTGAGTTCTTG+TGG + chr8.3:6002250-6002269 MS.gene061243:CDS 40.0%
!! GCTATTGAAGCTTTGGAGAA+AGG + chr8.3:6002313-6002332 MS.gene061243:CDS 40.0%
AAAGGTTGAGAAATGGGGCT+AGG + chr8.3:6002230-6002249 MS.gene061243:CDS 45.0%
GACCCTAAGTTTGCAGAAGA+TGG + chr8.3:6002337-6002356 MS.gene061243:CDS 45.0%
GCACCATCTTCTGCAAACTT+AGG - chr8.3:6002343-6002362 None:intergenic 45.0%
TTCTCAACCTTTGTAGCTGG+TGG - chr8.3:6002222-6002241 None:intergenic 45.0%
! AACAGGGCTAGCACAAATCA+TGG + chr8.3:6002153-6002172 MS.gene061243:CDS 45.0%
GCATATTCTGGTACCTCTGC+TGG + chr8.3:6002124-6002143 MS.gene061243:CDS 50.0%
! ACCTCTGCTGGTGATGTAAC+AGG + chr8.3:6002136-6002155 MS.gene061243:CDS 50.0%
! CCTCTGCTGGTGATGTAACA+GGG + chr8.3:6002137-6002156 MS.gene061243:CDS 50.0%
CCCTGTTACATCACCAGCAG+AGG - chr8.3:6002140-6002159 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 6001944 6002492 6001944 ID=MS.gene061243
chr8.3 mRNA 6001944 6002492 6001944 ID=MS.gene061243.t1;Parent=MS.gene061243
chr8.3 exon 6001944 6002492 6001944 ID=MS.gene061243.t1.exon1;Parent=MS.gene061243.t1
chr8.3 CDS 6001944 6002492 6001944 ID=cds.MS.gene061243.t1;Parent=MS.gene061243.t1
Gene Sequence

>MS.gene061243

ATGACAAATTTTAAGCCTCTAACTCTTTGCTTATTCCTTCAAGCTATTGTTATCGTAATTTCAATACCAACAATCCAAAGCAGAAGTACTAATTTCCCTTCAAAGGATGAAAAACTATCACTCATAGAAAACACTTGCAAAAAAACACCAAATTACAATGTTTGCCTTCAGTCTTTGAAGGCATATTCTGGTACCTCTGCTGGTGATGTAACAGGGCTAGCACAAATCATGGTGAAAGTGATGAAAGCAAAAGCAAACGATGGATTAAATAGAATCCACCAGCTACAAAGGTTGAGAAATGGGGCTAGGAAAGCTTTGAGTTCTTGTGGTGACAAATACAGAGCAATTTTGATAGCTGATATACCTCAAGCTATTGAAGCTTTGGAGAAAGGGGACCCTAAGTTTGCAGAAGATGGTGCAAATGATGCTGCTAATGAGGCTACTTATTGTGAGAGTGAGTTCAATGGGAAATCTCCACTCACAAAACAGAACAATGCTATGCATGATGTTTCAGCTGTTACAGCATCTATTGTTAGACAATTACTCTAG

Protein sequence

>MS.gene061243.t1

MTNFKPLTLCLFLQAIVIVISIPTIQSRSTNFPSKDEKLSLIENTCKKTPNYNVCLQSLKAYSGTSAGDVTGLAQIMVKVMKAKANDGLNRIHQLQRLRNGARKALSSCGDKYRAILIADIPQAIEALEKGDPKFAEDGANDAANEATYCESEFNGKSPLTKQNNAMHDVSAVTASIVRQLL