AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene33496


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATATGCTTCAAGGAATT+TGG 0.182257 4.2:+85748726 MS.gene33496:CDS
ACACTTTGAATACATCAGAT+TGG 0.462336 4.2:+85748793 MS.gene33496:CDS
CTTCGTGGATATATGCTTCA+AGG 0.473903 4.2:+85748719 MS.gene33496:CDS
TTGGAGACAGAGTAAAGCAT+TGG 0.480355 4.2:+85748745 MS.gene33496:CDS
TGGAGACAGAGTAAAGCATT+GGG 0.508305 4.2:+85748746 MS.gene33496:CDS
CAGCTGCATGAGCAAGAAGT+TGG 0.509222 4.2:-85748833 None:intergenic
GAAGTTGGTTGTGTGTGGCC+TGG 0.537777 4.2:-85748818 None:intergenic
GTATTCAAAGTGTAAGAGAA+TGG 0.570526 4.2:-85748783 None:intergenic
GAATACATCAGATTGGTACC+AGG 0.597455 4.2:+85748800 MS.gene33496:CDS
AGCAAGAAGTTGGTTGTGTG+TGG 0.636210 4.2:-85748823 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACACTTTGAATACATCAGAT+TGG + chr4.2:85748793-85748812 MS.gene33496:CDS 30.0%
GATATATGCTTCAAGGAATT+TGG + chr4.2:85748726-85748745 MS.gene33496:CDS 30.0%
GTATTCAAAGTGTAAGAGAA+TGG - chr4.2:85748786-85748805 None:intergenic 30.0%
!! AGATGATTTTAAGGACTTCG+TGG + chr4.2:85748704-85748723 MS.gene33496:CDS 35.0%
CTTCGTGGATATATGCTTCA+AGG + chr4.2:85748719-85748738 MS.gene33496:CDS 40.0%
GAATACATCAGATTGGTACC+AGG + chr4.2:85748800-85748819 MS.gene33496:CDS 40.0%
TTGGAGACAGAGTAAAGCAT+TGG + chr4.2:85748745-85748764 MS.gene33496:CDS 40.0%
! TGGAGACAGAGTAAAGCATT+GGG + chr4.2:85748746-85748765 MS.gene33496:CDS 40.0%
! AGCAAGAAGTTGGTTGTGTG+TGG - chr4.2:85748826-85748845 None:intergenic 45.0%
! GAAGTTGGTTGTGTGTGGCC+TGG - chr4.2:85748821-85748840 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 85748704 85748854 85748704 ID=MS.gene33496
chr4.2 mRNA 85748704 85748854 85748704 ID=MS.gene33496.t1;Parent=MS.gene33496
chr4.2 exon 85748704 85748854 85748704 ID=MS.gene33496.t1.exon1;Parent=MS.gene33496.t1
chr4.2 CDS 85748704 85748854 85748704 ID=cds.MS.gene33496.t1;Parent=MS.gene33496.t1
Gene Sequence

>MS.gene33496

AGATGATTTTAAGGACTTCGTGGATATATGCTTCAAGGAATTTGGAGACAGAGTAAAGCATTGGGTTACTTTTAATGAGCCATTCTCTTACACTTTGAATACATCAGATTGGTACCAGGCCACACACAACCAACTTCTTGCTCATGCAGCT

Protein sequence

>MS.gene33496.t1

RUFUGLRGYMLQGIWRQSKALGYFUUAILLHFEYIRLVPGHTQPTSCSCS