AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene36355


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36355.t1 MTR_2g101120 96.410 195 7 0 1 195 1 195 6.13e-143 396
MS.gene36355.t1 MTR_2g101130 83.920 199 27 2 1 194 1 199 1.74e-112 319
MS.gene36355.t1 MTR_8g014030 67.857 196 54 5 1 194 1 189 1.46e-92 268
MS.gene36355.t1 MTR_8g014010 62.371 194 62 5 1 192 1 185 7.73e-80 236
MS.gene36355.t1 MTR_8g014020 64.085 142 42 5 55 194 1 135 2.23e-56 175
MS.gene36355.t1 MTR_6g088545 40.933 193 87 7 7 194 10 180 5.32e-39 132
MS.gene36355.t1 MTR_6g088525 42.487 193 83 9 7 194 10 179 2.48e-38 131
MS.gene36355.t1 MTR_6g088505 41.753 194 84 9 7 194 10 180 1.60e-36 126
MS.gene36355.t1 MTR_6g088565 37.824 193 93 6 7 194 10 180 1.48e-35 124
MS.gene36355.t1 MTR_8g102660 35.961 203 93 7 1 195 3 176 5.02e-32 114
MS.gene36355.t1 MTR_0016s0060 37.989 179 83 5 22 194 1 157 1.66e-28 105
MS.gene36355.t1 MTR_0016s0050 38.150 173 70 6 32 194 1 146 1.99e-25 97.1
MS.gene36355.t1 MTR_7g084690 34.906 212 112 13 1 194 1 204 1.58e-21 88.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene36355.t1 AT1G52140 46.009 213 84 9 1 194 1 201 1.74e-51 165
MS.gene36355.t1 AT3G16330 45.327 214 79 10 1 194 1 196 1.28e-44 147
MS.gene36355.t1 AT4G29110 40.306 196 89 7 1 194 14 183 1.00e-30 111

Find 64 sgRNAs with CRISPR-Local

Find 68 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCTGCGCTGCCTGGATT+TGG 0.199261 2.2:+2280174 MS.gene36355:CDS
TGCGGTGACGTTTCCCTATT+GGG 0.266243 2.2:-2280029 None:intergenic
CTCCTGCGCTGCCTGGATTT+GGG 0.297032 2.2:+2280175 MS.gene36355:CDS
CTGCGGTGACGTTTCCCTAT+TGG 0.315623 2.2:-2280030 None:intergenic
TCAAGCTTTGCTTCCTCAAC+TGG 0.321584 2.2:-2280317 None:intergenic
TGTGGTGATGGTGGAACATT+AGG 0.346556 2.2:-2279909 None:intergenic
CCCTCAATTGCCTTACCATT+GGG 0.354815 2.2:-2280203 None:intergenic
TTCTCATCATCTTCTCTTAG+AGG 0.359691 2.2:-2280243 None:intergenic
ATCAAAGAGAGTGTGGAATA+TGG 0.384105 2.2:+2279774 MS.gene36355:CDS
CTGCTGAAGAATTCATAAAC+AGG 0.405304 2.2:+2280286 MS.gene36355:CDS
CCCAACCACTTCTTCCCAAT+AGG 0.431411 2.2:+2280015 MS.gene36355:CDS
GAAGCATCTCCTGCGCTGCC+TGG 0.434989 2.2:+2280168 MS.gene36355:CDS
CCAACCACTTCTTCCCAATA+GGG 0.449763 2.2:+2280016 MS.gene36355:CDS
GAGCGCAATGAAGGCAGTGC+TGG 0.452696 2.2:+2280116 MS.gene36355:CDS
ACTCCCTTGATGCGGTGAAT+TGG 0.458187 2.2:-2279969 None:intergenic
CCTATTGGGAAGAAGTGGTT+GGG 0.460023 2.2:-2280015 None:intergenic
AGGTGGAGCCGCCGTGGTTG+TGG 0.461991 2.2:-2279927 None:intergenic
AGCCGCCGTGGTTGTGGTGA+TGG 0.464409 2.2:-2279921 None:intergenic
GGCGTTTGAGCATCATGTTT+AGG 0.464704 2.2:-2279855 None:intergenic
ACCCTCAATTGCCTTACCAT+TGG 0.467623 2.2:-2280204 None:intergenic
GTCAAAGGGCAAACTAATGA+TGG 0.467955 2.2:+2279831 MS.gene36355:CDS
GCCTTCTTCATGTTGAGAAA+AGG 0.468789 2.2:+2279805 MS.gene36355:CDS
GTGTGAGCGGCGGCTTGAGG+TGG 0.473046 2.2:-2279944 None:intergenic
CGCCCAAATCCAGGCAGCGC+AGG 0.473511 2.2:-2280177 None:intergenic
GCCCAATGGTAAGGCAATTG+AGG 0.485585 2.2:+2280202 MS.gene36355:CDS
AGGTGGCGCTTGAGCGGAAG+TGG 0.487819 2.2:-2280067 None:intergenic
CCCTTGATGCGGTGAATTGG+TGG 0.491684 2.2:-2279966 None:intergenic
CACACTCTCTTTGATATCAC+TGG 0.497371 2.2:-2279766 None:intergenic
TCATCTTCTCTTAGAGGGAA+TGG 0.502641 2.2:-2280237 None:intergenic
CCCTATTGGGAAGAAGTGGT+TGG 0.503761 2.2:-2280016 None:intergenic
GGCGACAATGAGCGCAATGA+AGG 0.505967 2.2:+2280107 MS.gene36355:CDS
CCCAATGGTAAGGCAATTGA+GGG 0.507064 2.2:+2280203 MS.gene36355:CDS
AAACGCCGCAGCAAGCTCGC+CGG 0.510889 2.2:+2279871 MS.gene36355:CDS
ATTGGGCTACGCCCAAATCC+AGG 0.513644 2.2:-2280186 None:intergenic
GAAGGACGATCAAGTTGATA+AGG 0.519057 2.2:+2280263 MS.gene36355:CDS
CGCTCAAGCGCCACCTACAC+AGG 0.523001 2.2:+2280074 MS.gene36355:CDS
TCTCATCATCTTCTCTTAGA+GGG 0.530370 2.2:-2280242 None:intergenic
TTGAGAAAAGGCATGTCAAA+GGG 0.534014 2.2:+2279817 MS.gene36355:CDS
CTGTGTAGGTGGCGCTTGAG+CGG 0.542519 2.2:-2280073 None:intergenic
ACCACCAATTCACCGCATCA+AGG 0.543219 2.2:+2279965 MS.gene36355:CDS
GTGGTGTGAGCGGCGGCTTG+AGG 0.555757 2.2:-2279947 None:intergenic
GTTGAGAAAAGGCATGTCAA+AGG 0.559027 2.2:+2279816 MS.gene36355:CDS
ACCTCATCATCCTGTGTAGG+TGG 0.565659 2.2:-2280084 None:intergenic
GTGGAACATTAGGTTGGCAA+TGG 0.565737 2.2:-2279899 None:intergenic
GTTTCCCTATTGGGAAGAAG+TGG 0.569833 2.2:-2280020 None:intergenic
TGGAGAAGTTGTTGTGGCTG+CGG 0.571576 2.2:-2280047 None:intergenic
TTGGGCGTAGCCCAATGGTA+AGG 0.575007 2.2:+2280193 MS.gene36355:CDS
CGCCGTGGTTGTGGTGATGG+TGG 0.578705 2.2:-2279918 None:intergenic
TGGATTTGGGCGTAGCCCAA+TGG 0.585672 2.2:+2280188 MS.gene36355:CDS
TCAAAGGGCAAACTAATGAT+GGG 0.588410 2.2:+2279832 MS.gene36355:CDS
CAGTGATATCAAAGAGAGTG+TGG 0.593721 2.2:+2279767 MS.gene36355:CDS
TCTAAGAGAAGATGATGAGA+AGG 0.594411 2.2:+2280245 MS.gene36355:CDS
CGGTGAATTGGTGGTGTGAG+CGG 0.608131 2.2:-2279957 None:intergenic
TGAATTGGTGGTGTGAGCGG+CGG 0.628937 2.2:-2279954 None:intergenic
ACCTACACAGGATGATGAGG+TGG 0.634579 2.2:+2280086 MS.gene36355:CDS
CCACCAATTCACCGCATCAA+GGG 0.637439 2.2:+2279966 MS.gene36355:CDS
GGCTTGAGGTGGAGCCGCCG+TGG 0.640629 2.2:-2279933 None:intergenic
TTCCACCATCACCACAACCA+CGG 0.649090 2.2:+2279916 MS.gene36355:CDS
GCCACCTCATCATCCTGTGT+AGG 0.650227 2.2:-2280087 None:intergenic
CGGAAGTGGAGAAGTTGTTG+TGG 0.651141 2.2:-2280053 None:intergenic
GTGATGGTGGAACATTAGGT+TGG 0.654305 2.2:-2279905 None:intergenic
GCCACCTACACAGGATGATG+AGG 0.661418 2.2:+2280083 MS.gene36355:CDS
GAATTCATACTCCCTTGATG+CGG 0.696823 2.2:-2279977 None:intergenic
CACCATCACCACAACCACGG+CGG 0.721311 2.2:+2279919 MS.gene36355:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
CTGCTGAAGAATTCATAAAC+AGG + chr2.2:2280286-2280305 MS.gene36355:CDS 35.0%
TCAAAGGGCAAACTAATGAT+GGG + chr2.2:2279832-2279851 MS.gene36355:CDS 35.0%
TCTAAGAGAAGATGATGAGA+AGG + chr2.2:2280245-2280264 MS.gene36355:CDS 35.0%
TCTCATCATCTTCTCTTAGA+GGG - chr2.2:2280245-2280264 None:intergenic 35.0%
TTCTCATCATCTTCTCTTAG+AGG - chr2.2:2280246-2280265 None:intergenic 35.0%
! ATCAAAGAGAGTGTGGAATA+TGG + chr2.2:2279774-2279793 MS.gene36355:CDS 35.0%
! TTGAGAAAAGGCATGTCAAA+GGG + chr2.2:2279817-2279836 MS.gene36355:CDS 35.0%
CACACTCTCTTTGATATCAC+TGG - chr2.2:2279769-2279788 None:intergenic 40.0%
CAGTGATATCAAAGAGAGTG+TGG + chr2.2:2279767-2279786 MS.gene36355:CDS 40.0%
GAATTCATACTCCCTTGATG+CGG - chr2.2:2279980-2279999 None:intergenic 40.0%
GCCTTCTTCATGTTGAGAAA+AGG + chr2.2:2279805-2279824 MS.gene36355:CDS 40.0%
GTCAAAGGGCAAACTAATGA+TGG + chr2.2:2279831-2279850 MS.gene36355:CDS 40.0%
TCATCTTCTCTTAGAGGGAA+TGG - chr2.2:2280240-2280259 None:intergenic 40.0%
! GCCTTTTCTCAACATGAAGA+AGG - chr2.2:2279809-2279828 None:intergenic 40.0%
! GTTGAGAAAAGGCATGTCAA+AGG + chr2.2:2279816-2279835 MS.gene36355:CDS 40.0%
!! GAAGGACGATCAAGTTGATA+AGG + chr2.2:2280263-2280282 MS.gene36355:CDS 40.0%
ACCCTCAATTGCCTTACCAT+TGG - chr2.2:2280207-2280226 None:intergenic 45.0%
CCAACCACTTCTTCCCAATA+GGG + chr2.2:2280016-2280035 MS.gene36355:CDS 45.0%
CCCAATGGTAAGGCAATTGA+GGG + chr2.2:2280203-2280222 MS.gene36355:CDS 45.0%
CCCTCAATTGCCTTACCATT+GGG - chr2.2:2280206-2280225 None:intergenic 45.0%
CCTATTGGGAAGAAGTGGTT+GGG - chr2.2:2280018-2280037 None:intergenic 45.0%
GGCGTTTGAGCATCATGTTT+AGG - chr2.2:2279858-2279877 None:intergenic 45.0%
GTTTCCCTATTGGGAAGAAG+TGG - chr2.2:2280023-2280042 None:intergenic 45.0%
TCAAGCTTTGCTTCCTCAAC+TGG - chr2.2:2280320-2280339 None:intergenic 45.0%
! ACAGGTTTTACAGCCAGTTG+AGG + chr2.2:2280304-2280323 MS.gene36355:CDS 45.0%
!! GTGATGGTGGAACATTAGGT+TGG - chr2.2:2279908-2279927 None:intergenic 45.0%
!! GTGGAACATTAGGTTGGCAA+TGG - chr2.2:2279902-2279921 None:intergenic 45.0%
!! TAGGTTGGCAATGGCTTTTC+CGG - chr2.2:2279893-2279912 None:intergenic 45.0%
!! TGTGGTGATGGTGGAACATT+AGG - chr2.2:2279912-2279931 None:intergenic 45.0%
ACCACCAATTCACCGCATCA+AGG + chr2.2:2279965-2279984 MS.gene36355:CDS 50.0%
ACCTACACAGGATGATGAGG+TGG + chr2.2:2280086-2280105 MS.gene36355:CDS 50.0%
ACCTCATCATCCTGTGTAGG+TGG - chr2.2:2280087-2280106 None:intergenic 50.0%
ACTCCCTTGATGCGGTGAAT+TGG - chr2.2:2279972-2279991 None:intergenic 50.0%
CCACCAATTCACCGCATCAA+GGG + chr2.2:2279966-2279985 MS.gene36355:CDS 50.0%
CCCAACCACTTCTTCCCAAT+AGG + chr2.2:2280015-2280034 MS.gene36355:CDS 50.0%
CCCTATTGGGAAGAAGTGGT+TGG - chr2.2:2280019-2280038 None:intergenic 50.0%
CGGAAGTGGAGAAGTTGTTG+TGG - chr2.2:2280056-2280075 None:intergenic 50.0%
GCCCAATGGTAAGGCAATTG+AGG + chr2.2:2280202-2280221 MS.gene36355:CDS 50.0%
TGCGGTGACGTTTCCCTATT+GGG - chr2.2:2280032-2280051 None:intergenic 50.0%
TGGAGAAGTTGTTGTGGCTG+CGG - chr2.2:2280050-2280069 None:intergenic 50.0%
TTCCACCATCACCACAACCA+CGG + chr2.2:2279916-2279935 MS.gene36355:CDS 50.0%
ATTGGGCTACGCCCAAATCC+AGG - chr2.2:2280189-2280208 None:intergenic 55.0%
CCCTTGATGCGGTGAATTGG+TGG - chr2.2:2279969-2279988 None:intergenic 55.0%
CTGCGGTGACGTTTCCCTAT+TGG - chr2.2:2280033-2280052 None:intergenic 55.0%
GCCACCTACACAGGATGATG+AGG + chr2.2:2280083-2280102 MS.gene36355:CDS 55.0%
GCCACCTCATCATCCTGTGT+AGG - chr2.2:2280090-2280109 None:intergenic 55.0%
GGCGACAATGAGCGCAATGA+AGG + chr2.2:2280107-2280126 MS.gene36355:CDS 55.0%
TGGATTTGGGCGTAGCCCAA+TGG + chr2.2:2280188-2280207 MS.gene36355:CDS 55.0%
TTGGGCGTAGCCCAATGGTA+AGG + chr2.2:2280193-2280212 MS.gene36355:CDS 55.0%
!! CGGTGAATTGGTGGTGTGAG+CGG - chr2.2:2279960-2279979 None:intergenic 55.0%
!! TGAATTGGTGGTGTGAGCGG+CGG - chr2.2:2279957-2279976 None:intergenic 55.0%
CACCATCACCACAACCACGG+CGG + chr2.2:2279919-2279938 MS.gene36355:CDS 60.0%
CTCCTGCGCTGCCTGGATTT+GGG + chr2.2:2280175-2280194 MS.gene36355:CDS 60.0%
CTGTGTAGGTGGCGCTTGAG+CGG - chr2.2:2280076-2280095 None:intergenic 60.0%
GAGCGCAATGAAGGCAGTGC+TGG + chr2.2:2280116-2280135 MS.gene36355:CDS 60.0%
TCTCCTGCGCTGCCTGGATT+TGG + chr2.2:2280174-2280193 MS.gene36355:CDS 60.0%
!!! CTTTTCCGGCGAGCTTGCTG+CGG - chr2.2:2279879-2279898 None:intergenic 60.0%
AAACGCCGCAGCAAGCTCGC+CGG + chr2.2:2279871-2279890 MS.gene36355:CDS 65.0%
AGCCGCCGTGGTTGTGGTGA+TGG - chr2.2:2279924-2279943 None:intergenic 65.0%
AGGTGGCGCTTGAGCGGAAG+TGG - chr2.2:2280070-2280089 None:intergenic 65.0%
CGCTCAAGCGCCACCTACAC+AGG + chr2.2:2280074-2280093 MS.gene36355:CDS 65.0%
! GAAGCATCTCCTGCGCTGCC+TGG + chr2.2:2280168-2280187 MS.gene36355:CDS 65.0%
!! CGCCGTGGTTGTGGTGATGG+TGG - chr2.2:2279921-2279940 None:intergenic 65.0%
AGGTGGAGCCGCCGTGGTTG+TGG - chr2.2:2279930-2279949 None:intergenic 70.0%
CGCCCAAATCCAGGCAGCGC+AGG - chr2.2:2280180-2280199 None:intergenic 70.0%
GTGGTGTGAGCGGCGGCTTG+AGG - chr2.2:2279950-2279969 None:intergenic 70.0%
GTGTGAGCGGCGGCTTGAGG+TGG - chr2.2:2279947-2279966 None:intergenic 70.0%
GGCTTGAGGTGGAGCCGCCG+TGG - chr2.2:2279936-2279955 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 2279751 2280347 2279751 ID=MS.gene36355
chr2.2 mRNA 2279751 2280347 2279751 ID=MS.gene36355.t1;Parent=MS.gene36355
chr2.2 exon 2279751 2280347 2279751 ID=MS.gene36355.t1.exon1;Parent=MS.gene36355.t1
chr2.2 CDS 2279751 2280347 2279751 ID=cds.MS.gene36355.t1;Parent=MS.gene36355.t1
Gene Sequence

>MS.gene36355

ATGGAAAGCAATCTACCAGTGATATCAAAGAGAGTGTGGAATATGGTACGTGTAGCCTTCTTCATGTTGAGAAAAGGCATGTCAAAGGGCAAACTAATGATGGGCCTAAACATGATGCTCAAACGCCGCAGCAAGCTCGCCGGAAAAGCCATTGCCAACCTAATGTTCCACCATCACCACAACCACGGCGGCTCCACCTCAAGCCGCCGCTCACACCACCAATTCACCGCATCAAGGGAGTATGAATTCAGCTGTAGCAACACTCCCAACCACTTCTTCCCAATAGGGAAACGTCACCGCAGCCACAACAACTTCTCCACTTCCGCTCAAGCGCCACCTACACAGGATGATGAGGTGGCGACAATGAGCGCAATGAAGGCAGTGCTGGAGATGCTTAGCAATGATCAATCGATTGTAGAAGCATCTCCTGCGCTGCCTGGATTTGGGCGTAGCCCAATGGTAAGGCAATTGAGGGTGACTGACTCACCATTCCCTCTAAGAGAAGATGATGAGAAGGACGATCAAGTTGATAAGGCTGCTGAAGAATTCATAAACAGGTTTTACAGCCAGTTGAGGAAGCAAAGCTTGATTGATTAA

Protein sequence

>MS.gene36355.t1

MESNLPVISKRVWNMVRVAFFMLRKGMSKGKLMMGLNMMLKRRSKLAGKAIANLMFHHHHNHGGSTSSRRSHHQFTASREYEFSCSNTPNHFFPIGKRHRSHNNFSTSAQAPPTQDDEVATMSAMKAVLEMLSNDQSIVEASPALPGFGRSPMVRQLRVTDSPFPLREDDEKDDQVDKAAEEFINRFYSQLRKQSLID