Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37663.t1 | XP_013454609.1 | 100 | 133 | 0 | 0 | 1 | 133 | 64 | 196 | 3.10E-70 | 274.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37663.t1 | Q1RMX9 | 35.9 | 128 | 79 | 3 | 3 | 130 | 78 | 202 | 6.2e-19 | 95.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37663.t1 | B7FLH3 | 100.0 | 133 | 0 | 0 | 1 | 133 | 64 | 196 | 2.2e-70 | 274.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene37663 | 0.841677 | 4.11E-58 | -1.69E-46 |
| MS.gene050386 | MS.gene37663 | 0.803089 | 4.12E-49 | -1.69E-46 |
| MS.gene050815 | MS.gene37663 | 0.800912 | 1.15E-48 | -1.69E-46 |
| MS.gene051243 | MS.gene37663 | 0.832559 | 8.82E-56 | -1.69E-46 |
| MS.gene051328 | MS.gene37663 | 0.808315 | 3.29E-50 | -1.69E-46 |
| MS.gene051452 | MS.gene37663 | 0.813864 | 2.06E-51 | -1.69E-46 |
| MS.gene051757 | MS.gene37663 | 0.818141 | 2.29E-52 | -1.69E-46 |
| MS.gene052286 | MS.gene37663 | 0.812621 | 3.87E-51 | -1.69E-46 |
| MS.gene052426 | MS.gene37663 | 0.81667 | 4.91E-52 | -1.69E-46 |
| MS.gene053008 | MS.gene37663 | 0.814895 | 1.22E-51 | -1.69E-46 |
| MS.gene053216 | MS.gene37663 | 0.806326 | 8.69E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene37663 | 0.832409 | 9.61E-56 | -1.69E-46 |
| MS.gene05517 | MS.gene37663 | 0.810088 | 1.37E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene37663 | 0.811644 | 6.32E-51 | -1.69E-46 |
| MS.gene057140 | MS.gene37663 | 0.830727 | 2.49E-55 | -1.69E-46 |
| MS.gene05795 | MS.gene37663 | 0.802819 | 4.68E-49 | -1.69E-46 |
| MS.gene058054 | MS.gene37663 | 0.811591 | 6.49E-51 | -1.69E-46 |
| MS.gene058055 | MS.gene37663 | 0.823691 | 1.21E-53 | -1.69E-46 |
| MS.gene058146 | MS.gene37663 | 0.804677 | 1.93E-49 | -1.69E-46 |
| MS.gene058906 | MS.gene37663 | 0.804468 | 2.13E-49 | -1.69E-46 |
| MS.gene059317 | MS.gene37663 | 0.818312 | 2.09E-52 | -1.69E-46 |
| MS.gene059842 | MS.gene37663 | 0.82731 | 1.68E-54 | -1.69E-46 |
| MS.gene060310 | MS.gene37663 | 0.802707 | 4.94E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene37663 | 0.819522 | 1.11E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene37663 | 0.812479 | 4.15E-51 | -1.69E-46 |
| MS.gene060803 | MS.gene37663 | 0.809963 | 1.46E-50 | -1.69E-46 |
| MS.gene060998 | MS.gene37663 | 0.826192 | 3.10E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37663.t1 | MTR_4g008540 | 100.000 | 133 | 0 | 0 | 1 | 133 | 64 | 196 | 2.55e-97 | 279 |
| MS.gene37663.t1 | MTR_4g116980 | 65.414 | 133 | 46 | 0 | 1 | 133 | 64 | 196 | 1.98e-62 | 191 |
| MS.gene37663.t1 | MTR_2g064415 | 24.427 | 131 | 97 | 2 | 1 | 131 | 86 | 214 | 4.66e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene37663.t1 | AT1G15860 | 81.203 | 133 | 25 | 0 | 1 | 133 | 64 | 196 | 1.33e-80 | 237 |
| MS.gene37663.t1 | AT1G15860 | 81.203 | 133 | 25 | 0 | 1 | 133 | 64 | 196 | 1.33e-80 | 237 |
| MS.gene37663.t1 | AT1G15860 | 76.056 | 142 | 25 | 1 | 1 | 133 | 64 | 205 | 1.43e-77 | 229 |
| MS.gene37663.t1 | AT3G12760 | 26.154 | 130 | 94 | 2 | 1 | 130 | 87 | 214 | 2.94e-12 | 62.0 |
Find 23 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATTACTTTCTCTTGTTT+TGG | 0.145418 | 4.2:+91216432 | MS.gene37663:CDS |
| GTTTACCGGTTAGACAATAT+TGG | 0.279270 | 4.2:-91216280 | None:intergenic |
| AGCTCAAGTCAGCTTATTTG+TGG | 0.374125 | 4.2:+91216468 | MS.gene37663:CDS |
| GCTTTCCAATATTGTCTAAC+CGG | 0.393111 | 4.2:+91216275 | MS.gene37663:CDS |
| ACAAATAAGCTGACTTGAGC+TGG | 0.417133 | 4.2:-91216466 | None:intergenic |
| CTTATTTGTGGAATATTTGA+AGG | 0.426551 | 4.2:+91216480 | MS.gene37663:CDS |
| TGTTTACCTCGTTGCAAAAC+CGG | 0.432049 | 4.2:-91216659 | None:intergenic |
| CGTAAACATGGATCAGTGGA+TGG | 0.509518 | 4.2:+91216629 | MS.gene37663:CDS |
| AGGTGGATCATACTGATTTG+AGG | 0.520944 | 4.2:+91215230 | MS.gene37663:CDS |
| GGAGAGGCTTAAAAGCACTA+AGG | 0.525006 | 4.2:+91216057 | MS.gene37663:CDS |
| TACAGTAAGTAAATTGAAGA+AGG | 0.531444 | 4.2:+91216085 | MS.gene37663:CDS |
| GGCACTACCAGACCTAGAAA+AGG | 0.541569 | 4.2:+91216106 | MS.gene37663:CDS |
| TGATTACAAGGTCGTAAACA+TGG | 0.566445 | 4.2:+91216617 | MS.gene37663:CDS |
| GAGAGGCTTAAAAGCACTAA+GGG | 0.601499 | 4.2:+91216058 | MS.gene37663:CDS |
| TCAGAATCAAAATGATTACA+AGG | 0.602312 | 4.2:+91216605 | MS.gene37663:intron |
| GTAAACATGGATCAGTGGAT+GGG | 0.607934 | 4.2:+91216630 | MS.gene37663:CDS |
| TAACAGGAAAATGAAATCTG+AGG | 0.620467 | 4.2:+91215346 | MS.gene37663:intron |
| TTATAGGACGAGTGGAGGAG+AGG | 0.626288 | 4.2:+91216041 | MS.gene37663:intron |
| CATCTAATGTGAAGGTCCGG+AGG | 0.636647 | 4.2:+91216222 | MS.gene37663:intron |
| AGGTCGTAAACATGGATCAG+TGG | 0.649284 | 4.2:+91216625 | MS.gene37663:CDS |
| AAAATGAAATCTGAGGAGCA+AGG | 0.670608 | 4.2:+91215353 | MS.gene37663:CDS |
| ACTACCAGACCTAGAAAAGG+AGG | 0.671613 | 4.2:+91216109 | MS.gene37663:CDS |
| ACTTTGTGCTGATATGGAGG+TGG | 0.689902 | 4.2:+91215213 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACACATATATTATTAAAATA+AGG | - | chr4.2:91215819-91215838 | None:intergenic | 10.0% |
| !! | CACATATATTATTAAAATAA+GGG | - | chr4.2:91215818-91215837 | None:intergenic | 10.0% |
| !!! | CCTTTTTTATTTTATTTTAA+GGG | + | chr4.2:91215614-91215633 | MS.gene37663:intron | 10.0% |
| !!! | TCCTTTTTTATTTTATTTTA+AGG | + | chr4.2:91215613-91215632 | MS.gene37663:intron | 10.0% |
| !!! | TTATTTAAATTTTAGAAGTT+TGG | + | chr4.2:91215434-91215453 | MS.gene37663:intron | 10.0% |
| !! | AAAAGCTTGTATTATAAATA+GGG | - | chr4.2:91215899-91215918 | None:intergenic | 15.0% |
| !! | ATATAAGAATACAATGTTAA+AGG | - | chr4.2:91215297-91215316 | None:intergenic | 15.0% |
| !! | CCCTTAAAATAAAATAAAAA+AGG | - | chr4.2:91215617-91215636 | None:intergenic | 15.0% |
| !! | TAAAAGCTTGTATTATAAAT+AGG | - | chr4.2:91215900-91215919 | None:intergenic | 15.0% |
| !!! | TTTTTTTATAATTTTACCAG+AGG | + | chr4.2:91216375-91216394 | MS.gene37663:intron | 15.0% |
| !! | GATGGAAATTATTATTTATG+AGG | + | chr4.2:91216159-91216178 | MS.gene37663:intron | 20.0% |
| !! | TAAGAAAAAGTTTCTACATT+TGG | - | chr4.2:91215678-91215697 | None:intergenic | 20.0% |
| !! | TAGAAATCTGAAAAATTTGA+CGG | - | chr4.2:91216248-91216267 | None:intergenic | 20.0% |
| !!! | TACAAGCTTTTATACATTAA+AGG | + | chr4.2:91215908-91215927 | MS.gene37663:intron | 20.0% |
| !!! | TTATCACCTGTATATTTTAT+AGG | + | chr4.2:91216025-91216044 | MS.gene37663:intron | 20.0% |
| ! | AAAATCACATCAAAGTTTAC+CGG | - | chr4.2:91216297-91216316 | None:intergenic | 25.0% |
| ! | ACATTAAAGGCTCAAATATT+TGG | + | chr4.2:91215921-91215940 | MS.gene37663:intron | 25.0% |
| ! | ATATTATTTATCTCACGGTT+TGG | + | chr4.2:91215516-91215535 | MS.gene37663:intron | 25.0% |
| ! | CTTATTTGTGGAATATTTGA+AGG | + | chr4.2:91216480-91216499 | MS.gene37663:CDS | 25.0% |
| ! | GAATATTTGAAGGTGAATAA+TGG | + | chr4.2:91216490-91216509 | MS.gene37663:intron | 25.0% |
| ! | GACTCATATTATTTATCTCA+CGG | + | chr4.2:91215511-91215530 | MS.gene37663:intron | 25.0% |
| ! | GATAAAAGTTATTCAGTACA+AGG | - | chr4.2:91216010-91216029 | None:intergenic | 25.0% |
| ! | TACAGTAAGTAAATTGAAGA+AGG | + | chr4.2:91216085-91216104 | MS.gene37663:CDS | 25.0% |
| ! | TCAGAATCAAAATGATTACA+AGG | + | chr4.2:91216605-91216624 | MS.gene37663:intron | 25.0% |
| !! | TTCATTTTCCTGTTACATTT+AGG | - | chr4.2:91215341-91215360 | None:intergenic | 25.0% |
| !!! | GAATTACTTTCTCTTGTTTT+GGG | + | chr4.2:91216433-91216452 | MS.gene37663:CDS | 25.0% |
| !!! | TGAATTACTTTCTCTTGTTT+TGG | + | chr4.2:91216432-91216451 | MS.gene37663:CDS | 25.0% |
| AAAGTTATTCAGTACAAGGA+TGG | - | chr4.2:91216006-91216025 | None:intergenic | 30.0% | |
| AACTTTGACATCTAATGTGA+AGG | + | chr4.2:91216214-91216233 | MS.gene37663:intron | 30.0% | |
| AAGTTATTCAGTACAAGGAT+GGG | - | chr4.2:91216005-91216024 | None:intergenic | 30.0% | |
| ACATGATAATTTACGTACCA+CGG | - | chr4.2:91215387-91215406 | None:intergenic | 30.0% | |
| ACTCGTCCTATAAAATATAC+AGG | - | chr4.2:91216034-91216053 | None:intergenic | 30.0% | |
| ATAATATATGTGTCGTGTTG+TGG | + | chr4.2:91215826-91215845 | MS.gene37663:intron | 30.0% | |
| GAACATAAGTTAAGTTCCAT+AGG | - | chr4.2:91215874-91215893 | None:intergenic | 30.0% | |
| TAACAGGAAAATGAAATCTG+AGG | + | chr4.2:91215346-91215365 | MS.gene37663:intron | 30.0% | |
| TAATCAAGCCTAAATGTAAC+AGG | + | chr4.2:91215330-91215349 | MS.gene37663:intron | 30.0% | |
| TTAAGTGATACTCTTCAAGA+TGG | + | chr4.2:91216141-91216160 | MS.gene37663:intron | 30.0% | |
| ! | TGTATATTTTATAGGACGAG+TGG | + | chr4.2:91216033-91216052 | MS.gene37663:intron | 30.0% |
| AAAATGAAATCTGAGGAGCA+AGG | + | chr4.2:91215353-91215372 | MS.gene37663:CDS | 35.0% | |
| ACAAGGATGGGAAAGAAATA+TGG | - | chr4.2:91215993-91216012 | None:intergenic | 35.0% | |
| ATAATATGAGTCATGCGTTG+TGG | - | chr4.2:91215503-91215522 | None:intergenic | 35.0% | |
| GCTTTCCAATATTGTCTAAC+CGG | + | chr4.2:91216275-91216294 | MS.gene37663:CDS | 35.0% | |
| TGATTACAAGGTCGTAAACA+TGG | + | chr4.2:91216617-91216636 | MS.gene37663:CDS | 35.0% | |
| ! | ATATTTTATAGGACGAGTGG+AGG | + | chr4.2:91216036-91216055 | MS.gene37663:intron | 35.0% |
| ! | GTAATCATACCTCCTTTTCT+AGG | - | chr4.2:91216121-91216140 | None:intergenic | 35.0% |
| ! | GTTTACCGGTTAGACAATAT+TGG | - | chr4.2:91216283-91216302 | None:intergenic | 35.0% |
| !! | AAATGTTAACTTGTGCCCTT+GGG | + | chr4.2:91215637-91215656 | MS.gene37663:intron | 35.0% |
| AGCTCAAGTCAGCTTATTTG+TGG | + | chr4.2:91216468-91216487 | MS.gene37663:CDS | 40.0% | |
| CACACTCTTGGCAACAATTA+AGG | - | chr4.2:91215558-91215577 | None:intergenic | 40.0% | |
| GGAACTAAGTACAGTTCCAT+AGG | + | chr4.2:91215455-91215474 | MS.gene37663:intron | 40.0% | |
| GTAAACATGGATCAGTGGAT+GGG | + | chr4.2:91216630-91216649 | MS.gene37663:CDS | 40.0% | |
| TATATGTGTCGTGTTGTGGT+TGG | + | chr4.2:91215830-91215849 | MS.gene37663:intron | 40.0% | |
| TGACATCTAATGTGAAGGTC+CGG | + | chr4.2:91216219-91216238 | MS.gene37663:intron | 40.0% | |
| TTCCTTAACTTATGCCCCAA+GGG | - | chr4.2:91215655-91215674 | None:intergenic | 40.0% | |
| ! | ATCAGTGGATGGGATTTTTC+CGG | + | chr4.2:91216640-91216659 | MS.gene37663:CDS | 40.0% |
| ! | GCTATGCAATCATGCAGTTT+TGG | + | chr4.2:91215965-91215984 | MS.gene37663:intron | 40.0% |
| !! | AATGTTAACTTGTGCCCTTG+GGG | + | chr4.2:91215638-91215657 | MS.gene37663:intron | 40.0% |
| !! | ACAAATAAGCTGACTTGAGC+TGG | - | chr4.2:91216469-91216488 | None:intergenic | 40.0% |
| !! | AGGTGGATCATACTGATTTG+AGG | + | chr4.2:91215230-91215249 | MS.gene37663:CDS | 40.0% |
| !! | ATGCTTTTCTGTTTCTCCTC+TGG | - | chr4.2:91216394-91216413 | None:intergenic | 40.0% |
| !! | GAAATGTTAACTTGTGCCCT+TGG | + | chr4.2:91215636-91215655 | MS.gene37663:intron | 40.0% |
| !! | GAGAGGCTTAAAAGCACTAA+GGG | + | chr4.2:91216058-91216077 | MS.gene37663:CDS | 40.0% |
| !! | TGAGGATTTTGATGCTTGCT+TGG | + | chr4.2:91215248-91215267 | MS.gene37663:CDS | 40.0% |
| !! | TTTATCTCACGGTTTGGTGA+AGG | + | chr4.2:91215522-91215541 | MS.gene37663:intron | 40.0% |
| ACAGTTCCATAGGTCCTGTA+CGG | + | chr4.2:91215465-91215484 | MS.gene37663:intron | 45.0% | |
| ACTACCAGACCTAGAAAAGG+AGG | + | chr4.2:91216109-91216128 | MS.gene37663:CDS | 45.0% | |
| AGGTCGTAAACATGGATCAG+TGG | + | chr4.2:91216625-91216644 | MS.gene37663:CDS | 45.0% | |
| AGTGCTGCATTTCACACTCT+TGG | - | chr4.2:91215570-91215589 | None:intergenic | 45.0% | |
| CGTAAACATGGATCAGTGGA+TGG | + | chr4.2:91216629-91216648 | MS.gene37663:CDS | 45.0% | |
| CTGAAAAATTTGACGGCCTC+CGG | - | chr4.2:91216241-91216260 | None:intergenic | 45.0% | |
| GTTCCTTAACTTATGCCCCA+AGG | - | chr4.2:91215656-91215675 | None:intergenic | 45.0% | |
| TATGAGTCATGCGTTGTGGT+CGG | - | chr4.2:91215499-91215518 | None:intergenic | 45.0% | |
| ! | CATACCTCCTTTTCTAGGTC+TGG | - | chr4.2:91216116-91216135 | None:intergenic | 45.0% |
| ! | GGAGCAAGGATATTTTACCG+TGG | + | chr4.2:91215367-91215386 | MS.gene37663:CDS | 45.0% |
| !! | CATGTTAACTTGTGCCCTGT+GGG | - | chr4.2:91215794-91215813 | None:intergenic | 45.0% |
| !! | GGAGAGGCTTAAAAGCACTA+AGG | + | chr4.2:91216057-91216076 | MS.gene37663:CDS | 45.0% |
| !! | TCATGTTAACTTGTGCCCTG+TGG | - | chr4.2:91215795-91215814 | None:intergenic | 45.0% |
| AACCTTCCGTACAGGACCTA+TGG | - | chr4.2:91215474-91215493 | None:intergenic | 50.0% | |
| ATGTGTCGTGTTGTGGTTGG+AGG | + | chr4.2:91215833-91215852 | MS.gene37663:intron | 50.0% | |
| CATCTAATGTGAAGGTCCGG+AGG | + | chr4.2:91216222-91216241 | MS.gene37663:intron | 50.0% | |
| GACAGCAAGGTTAGGACCTA+TGG | + | chr4.2:91215855-91215874 | MS.gene37663:intron | 50.0% | |
| GGCACTACCAGACCTAGAAA+AGG | + | chr4.2:91216106-91216125 | MS.gene37663:CDS | 50.0% | |
| TGCCCTTGGGGCATAAGTTA+AGG | + | chr4.2:91215650-91215669 | MS.gene37663:intron | 50.0% | |
| TTATAGGACGAGTGGAGGAG+AGG | + | chr4.2:91216041-91216060 | MS.gene37663:intron | 50.0% | |
| TTCCATAGGTCCTGTACGGA+AGG | + | chr4.2:91215469-91215488 | MS.gene37663:intron | 50.0% | |
| !! | CTCCTTAACTTGTGCCCACA+GGG | + | chr4.2:91215777-91215796 | MS.gene37663:intron | 50.0% |
| GGTCGGAAAACCTTCCGTAC+AGG | - | chr4.2:91215482-91215501 | None:intergenic | 55.0% | |
| GGTTGGAGGACAGCAAGGTT+AGG | + | chr4.2:91215847-91215866 | MS.gene37663:intron | 55.0% | |
| GTTGTGGTTGGAGGACAGCA+AGG | + | chr4.2:91215842-91215861 | MS.gene37663:intron | 55.0% | |
| TGCCCTGTGGGCACAAGTTA+AGG | - | chr4.2:91215782-91215801 | None:intergenic | 55.0% | |
| !! | GCTCCTTAACTTGTGCCCAC+AGG | + | chr4.2:91215776-91215795 | MS.gene37663:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 91215226 | 91216674 | 91215226 | ID=MS.gene37663 |
| chr4.2 | mRNA | 91215226 | 91216674 | 91215226 | ID=MS.gene37663.t1;Parent=MS.gene37663 |
| chr4.2 | exon | 91215226 | 91215269 | 91215226 | ID=MS.gene37663.t1.exon1;Parent=MS.gene37663.t1 |
| chr4.2 | CDS | 91215226 | 91215269 | 91215226 | ID=cds.MS.gene37663.t1;Parent=MS.gene37663.t1 |
| chr4.2 | exon | 91215352 | 91215388 | 91215352 | ID=MS.gene37663.t1.exon2;Parent=MS.gene37663.t1 |
| chr4.2 | CDS | 91215352 | 91215388 | 91215352 | ID=cds.MS.gene37663.t1;Parent=MS.gene37663.t1 |
| chr4.2 | exon | 91216047 | 91216130 | 91216047 | ID=MS.gene37663.t1.exon3;Parent=MS.gene37663.t1 |
| chr4.2 | CDS | 91216047 | 91216130 | 91216047 | ID=cds.MS.gene37663.t1;Parent=MS.gene37663.t1 |
| chr4.2 | exon | 91216236 | 91216296 | 91216236 | ID=MS.gene37663.t1.exon4;Parent=MS.gene37663.t1 |
| chr4.2 | CDS | 91216236 | 91216296 | 91216236 | ID=cds.MS.gene37663.t1;Parent=MS.gene37663.t1 |
| chr4.2 | exon | 91216395 | 91216501 | 91216395 | ID=MS.gene37663.t1.exon5;Parent=MS.gene37663.t1 |
| chr4.2 | CDS | 91216395 | 91216501 | 91216395 | ID=cds.MS.gene37663.t1;Parent=MS.gene37663.t1 |
| chr4.2 | exon | 91216609 | 91216674 | 91216609 | ID=MS.gene37663.t1.exon6;Parent=MS.gene37663.t1 |
| chr4.2 | CDS | 91216609 | 91216674 | 91216609 | ID=cds.MS.gene37663.t1;Parent=MS.gene37663.t1 |
| Gene Sequence |
| Protein sequence |
