Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41380.t1 | AES62588.1 | 96.9 | 645 | 18 | 2 | 1 | 645 | 1 | 643 | 0.00E+00 | 1213.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41380.t1 | Q21657 | 23.6 | 424 | 242 | 12 | 169 | 582 | 41 | 392 | 1.0e-13 | 80.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41380.t1 | G7IC19 | 96.9 | 645 | 18 | 2 | 1 | 645 | 1 | 643 | 0.0e+00 | 1213.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene41380 | 0.804984 | 1.66E-49 | -1.69E-46 |
| MS.gene049958 | MS.gene41380 | 0.80799 | 3.86E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene41380 | 0.828322 | 9.59E-55 | -1.69E-46 |
| MS.gene050657 | MS.gene41380 | 0.832991 | 6.89E-56 | -1.69E-46 |
| MS.gene050759 | MS.gene41380 | 0.85762 | 1.44E-62 | -1.69E-46 |
| MS.gene050826 | MS.gene41380 | 0.816199 | 6.26E-52 | -1.69E-46 |
| MS.gene050941 | MS.gene41380 | 0.802419 | 5.66E-49 | -1.69E-46 |
| MS.gene050962 | MS.gene41380 | 0.848005 | 8.07E-60 | -1.69E-46 |
| MS.gene051198 | MS.gene41380 | 0.8045 | 2.10E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene41380 | 0.803257 | 3.80E-49 | -1.69E-46 |
| MS.gene051490 | MS.gene41380 | 0.839001 | 2.06E-57 | -1.69E-46 |
| MS.gene051800 | MS.gene41380 | 0.81652 | 5.30E-52 | -1.69E-46 |
| MS.gene052016 | MS.gene41380 | 0.823981 | 1.03E-53 | -1.69E-46 |
| MS.gene052463 | MS.gene41380 | 0.829638 | 4.60E-55 | -1.69E-46 |
| MS.gene052493 | MS.gene41380 | 0.840961 | 6.34E-58 | -1.69E-46 |
| MS.gene052896 | MS.gene41380 | 0.812544 | 4.02E-51 | -1.69E-46 |
| MS.gene053621 | MS.gene41380 | 0.80754 | 4.81E-50 | -1.69E-46 |
| MS.gene05423 | MS.gene41380 | 0.816855 | 4.46E-52 | -1.69E-46 |
| MS.gene054467 | MS.gene41380 | 0.827322 | 1.67E-54 | -1.69E-46 |
| MS.gene054468 | MS.gene41380 | 0.82177 | 3.38E-53 | -1.69E-46 |
| MS.gene055465 | MS.gene41380 | 0.808768 | 2.63E-50 | -1.69E-46 |
| MS.gene055466 | MS.gene41380 | 0.819406 | 1.18E-52 | -1.69E-46 |
| MS.gene055469 | MS.gene41380 | 0.808877 | 2.50E-50 | -1.69E-46 |
| MS.gene055861 | MS.gene41380 | -0.803011 | 4.27E-49 | -1.69E-46 |
| MS.gene055864 | MS.gene41380 | 0.836674 | 8.15E-57 | -1.69E-46 |
| MS.gene055926 | MS.gene41380 | 0.836103 | 1.14E-56 | -1.69E-46 |
| MS.gene056174 | MS.gene41380 | 0.829804 | 4.19E-55 | -1.69E-46 |
| MS.gene057915 | MS.gene41380 | 0.809537 | 1.80E-50 | -1.69E-46 |
| MS.gene058429 | MS.gene41380 | 0.809155 | 2.18E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene41380 | 0.805705 | 1.17E-49 | -1.69E-46 |
| MS.gene059283 | MS.gene41380 | 0.820973 | 5.17E-53 | -1.69E-46 |
| MS.gene060122 | MS.gene41380 | -0.862651 | 4.36E-64 | -1.69E-46 |
| MS.gene060125 | MS.gene41380 | -0.844618 | 6.76E-59 | -1.69E-46 |
| MS.gene060322 | MS.gene41380 | 0.803772 | 2.97E-49 | -1.69E-46 |
| MS.gene060373 | MS.gene41380 | 0.843432 | 1.41E-58 | -1.69E-46 |
| MS.gene06052 | MS.gene41380 | 0.887458 | 1.40E-72 | -1.69E-46 |
| MS.gene060802 | MS.gene41380 | 0.813395 | 2.62E-51 | -1.69E-46 |
| MS.gene060932 | MS.gene41380 | 0.840307 | 9.41E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41380.t1 | MTR_1g101790 | 97.829 | 645 | 12 | 2 | 1 | 645 | 1 | 643 | 0.0 | 1303 |
| MS.gene41380.t1 | MTR_1g101790 | 97.792 | 634 | 12 | 2 | 1 | 634 | 1 | 632 | 0.0 | 1280 |
| MS.gene41380.t1 | MTR_6g089580 | 66.868 | 661 | 164 | 13 | 2 | 645 | 6 | 628 | 0.0 | 838 |
| MS.gene41380.t1 | MTR_6g464840 | 66.415 | 661 | 167 | 13 | 2 | 645 | 6 | 628 | 0.0 | 838 |
| MS.gene41380.t1 | MTR_4g102620 | 50.466 | 644 | 273 | 12 | 8 | 645 | 12 | 615 | 0.0 | 600 |
| MS.gene41380.t1 | MTR_8g079020 | 47.130 | 331 | 146 | 5 | 50 | 376 | 95 | 400 | 7.64e-90 | 299 |
| MS.gene41380.t1 | MTR_8g079020 | 41.500 | 200 | 111 | 3 | 434 | 628 | 727 | 925 | 1.22e-35 | 144 |
| MS.gene41380.t1 | MTR_8g079040 | 47.826 | 322 | 139 | 5 | 59 | 376 | 104 | 400 | 1.04e-89 | 299 |
| MS.gene41380.t1 | MTR_8g079040 | 42.365 | 203 | 111 | 3 | 434 | 631 | 723 | 924 | 1.56e-38 | 153 |
| MS.gene41380.t1 | MTR_3g099200 | 30.500 | 200 | 117 | 8 | 174 | 366 | 134 | 318 | 2.24e-18 | 87.0 |
| MS.gene41380.t1 | MTR_3g099200 | 31.250 | 192 | 110 | 8 | 174 | 358 | 181 | 357 | 2.97e-18 | 87.4 |
| MS.gene41380.t1 | MTR_4g102890 | 31.183 | 186 | 113 | 8 | 174 | 358 | 91 | 262 | 3.74e-16 | 79.3 |
| MS.gene41380.t1 | MTR_4g102890 | 31.183 | 186 | 113 | 8 | 174 | 358 | 126 | 297 | 5.08e-16 | 79.7 |
| MS.gene41380.t1 | MTR_4g102890 | 31.183 | 186 | 113 | 8 | 174 | 358 | 174 | 345 | 6.33e-16 | 80.1 |
| MS.gene41380.t1 | MTR_4g104060 | 27.568 | 185 | 119 | 7 | 174 | 358 | 158 | 327 | 3.19e-14 | 75.1 |
| MS.gene41380.t1 | MTR_4g104060 | 27.568 | 185 | 119 | 7 | 174 | 358 | 172 | 341 | 3.50e-14 | 75.1 |
| MS.gene41380.t1 | MTR_4g030930 | 26.147 | 218 | 128 | 7 | 178 | 369 | 34 | 244 | 5.54e-12 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene41380.t1 | AT5G51920 | 47.840 | 625 | 240 | 12 | 12 | 634 | 16 | 556 | 0.0 | 539 |
| MS.gene41380.t1 | AT4G22980 | 40.978 | 593 | 258 | 13 | 56 | 640 | 49 | 557 | 7.25e-135 | 407 |
| MS.gene41380.t1 | AT5G66950 | 44.335 | 406 | 190 | 9 | 50 | 446 | 93 | 471 | 1.18e-95 | 313 |
| MS.gene41380.t1 | AT5G66950 | 47.399 | 173 | 76 | 3 | 463 | 630 | 693 | 855 | 3.91e-37 | 149 |
| MS.gene41380.t1 | AT4G37100 | 45.722 | 374 | 166 | 8 | 63 | 426 | 110 | 456 | 4.71e-93 | 307 |
| MS.gene41380.t1 | AT4G37100 | 46.286 | 175 | 89 | 3 | 455 | 627 | 704 | 875 | 6.48e-36 | 145 |
| MS.gene41380.t1 | AT2G23520 | 42.967 | 391 | 182 | 9 | 50 | 426 | 95 | 458 | 1.51e-90 | 300 |
| MS.gene41380.t1 | AT2G23520 | 43.478 | 184 | 96 | 3 | 452 | 627 | 693 | 876 | 1.88e-32 | 134 |
| MS.gene41380.t1 | AT1G16540 | 26.609 | 233 | 126 | 9 | 171 | 369 | 78 | 299 | 2.38e-13 | 73.9 |
| MS.gene41380.t1 | AT1G16540 | 26.609 | 233 | 126 | 9 | 171 | 369 | 78 | 299 | 2.53e-13 | 73.9 |
| MS.gene41380.t1 | AT1G16540 | 26.609 | 233 | 126 | 9 | 171 | 369 | 78 | 299 | 3.21e-13 | 73.6 |
Find 111 sgRNAs with CRISPR-Local
Find 129 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATCATCTTCTTCAATT+TGG | 0.166875 | 1.4:-78966159 | None:intergenic |
| ATGGATTTCTGCATCATATT+TGG | 0.188983 | 1.4:+78967041 | MS.gene41380:CDS |
| GCATTGTGAAGAACTGTTTC+TGG | 0.195173 | 1.4:-78965487 | None:intergenic |
| GTTCCCTTAGTCAAGATTTA+TGG | 0.214330 | 1.4:+78966896 | MS.gene41380:CDS |
| AGATTCATGGTTTGTGAATT+TGG | 0.224461 | 1.4:-78965553 | None:intergenic |
| AAAGAAAGATAGAGATGATT+TGG | 0.248446 | 1.4:+78967144 | MS.gene41380:CDS |
| TACTGCTGCATTGAGCTTCT+TGG | 0.252366 | 1.4:+78967180 | MS.gene41380:CDS |
| GAGGAAGGTACCTTATAAAT+TGG | 0.252586 | 1.4:+78966828 | MS.gene41380:CDS |
| TAGAAGATTTCTTGACAAAA+AGG | 0.259026 | 1.4:-78966436 | None:intergenic |
| AGATTAAATTGCGTACTTTC+TGG | 0.260689 | 1.4:-78966506 | None:intergenic |
| CTGAAACTATGGAGGCAAAA+AGG | 0.271150 | 1.4:+78966690 | MS.gene41380:CDS |
| TCAGAACCTAGTGGAACTTT+TGG | 0.273310 | 1.4:-78966671 | None:intergenic |
| GAGCAAGATATCCTTATCTT+TGG | 0.278624 | 1.4:+78966255 | MS.gene41380:CDS |
| ATGTTGTTGACGTTGATAAT+AGG | 0.286526 | 1.4:-78965730 | None:intergenic |
| CCACTTCATTCAAGGGTAAC+AGG | 0.289081 | 1.4:+78966233 | MS.gene41380:CDS |
| AGAAAGATGGTAATATTCTT+TGG | 0.291187 | 1.4:-78965661 | None:intergenic |
| TTTAGTATTTCCTACAAAAC+TGG | 0.297399 | 1.4:+78965834 | MS.gene41380:CDS |
| TTGATTGATGCTTGTGCATT+AGG | 0.298332 | 1.4:+78966311 | MS.gene41380:CDS |
| CCTCTATCAAATCTTATCTT+TGG | 0.300166 | 1.4:-78966920 | None:intergenic |
| GGAGAAAATCCATCTGGTTT+TGG | 0.300460 | 1.4:+78966410 | MS.gene41380:CDS |
| CCAAAACTGTCCATGTCTTT+TGG | 0.314322 | 1.4:-78966335 | None:intergenic |
| AGTTTACAAGCCAATTTATA+AGG | 0.318414 | 1.4:-78966838 | None:intergenic |
| TCCACTAGGTTCTGAAACTA+TGG | 0.320483 | 1.4:+78966679 | MS.gene41380:CDS |
| TTCTGAAAATGATTATTTCA+TGG | 0.325758 | 1.4:+78965944 | MS.gene41380:CDS |
| TTGAAGACTTTATTGCTTCA+TGG | 0.326674 | 1.4:+78965861 | MS.gene41380:CDS |
| TTGGATCAAGTGGACTCTTT+AGG | 0.327381 | 1.4:+78966785 | MS.gene41380:CDS |
| TTGAAGATGTGTATAAACTA+TGG | 0.329565 | 1.4:+78967209 | MS.gene41380:CDS |
| TGAAGAACTGTTTCTGGGTT+TGG | 0.332280 | 1.4:-78965481 | None:intergenic |
| AAGGGAACCCCTTCATCATT+AGG | 0.351025 | 1.4:-78966881 | None:intergenic |
| CCAAAAGACATGGACAGTTT+TGG | 0.355281 | 1.4:+78966335 | MS.gene41380:CDS |
| CTATCAGAAGATTATTCTTC+GGG | 0.355296 | 1.4:+78966527 | MS.gene41380:CDS |
| TGTCTTGATTACATTGGTAT+TGG | 0.360444 | 1.4:+78965699 | MS.gene41380:CDS |
| TAGCTTTCAATGTTTATGAT+TGG | 0.366950 | 1.4:+78966951 | MS.gene41380:CDS |
| TAACATTGAATGCAGGTGTT+TGG | 0.367839 | 1.4:+78966766 | MS.gene41380:CDS |
| CATTGTGAAGAACTGTTTCT+GGG | 0.378112 | 1.4:-78965486 | None:intergenic |
| TCCTGCTCTTGTAAAATAGA+TGG | 0.379943 | 1.4:-78966581 | None:intergenic |
| AAGAAAGATAGAGATGATTT+GGG | 0.382430 | 1.4:+78967145 | MS.gene41380:CDS |
| AATGAACAAATGAGAAAATC+TGG | 0.384998 | 1.4:-78966374 | None:intergenic |
| TGATGATTTGATGAACAATA+AGG | 0.394314 | 1.4:-78965433 | None:intergenic |
| GCAAGTTTCTGTACTAAAAC+AGG | 0.396354 | 1.4:-78966992 | None:intergenic |
| TCTATCAGAAGATTATTCTT+CGG | 0.408300 | 1.4:+78966526 | MS.gene41380:CDS |
| TTGTATTTCCACTTCATTCA+AGG | 0.408976 | 1.4:+78966225 | MS.gene41380:CDS |
| TTTGTAGGAAATACTAAAGA+AGG | 0.415912 | 1.4:-78965829 | None:intergenic |
| GGTAATAATCAAGTAGGAAC+TGG | 0.437830 | 1.4:+78966548 | MS.gene41380:CDS |
| TGGTTTGTGAATTTGGTGTT+TGG | 0.441750 | 1.4:-78965546 | None:intergenic |
| AGGGAACCCCTTCATCATTA+GGG | 0.445414 | 1.4:-78966880 | None:intergenic |
| CTTGAATCCTCCACTTGTGC+AGG | 0.447757 | 1.4:+78966467 | MS.gene41380:CDS |
| TTGAGTCTGCAATGAGGAAA+AGG | 0.451252 | 1.4:+78965901 | MS.gene41380:CDS |
| CAATGTAATCAAGACAAGAT+TGG | 0.453041 | 1.4:-78965692 | None:intergenic |
| TGGACCATAAATCTTGACTA+AGG | 0.453919 | 1.4:-78966900 | None:intergenic |
| AGCTTCTTACAAGATTGAAA+TGG | 0.456571 | 1.4:-78966017 | None:intergenic |
| GTTGATTGAATTCTAAGCCT+TGG | 0.461468 | 1.4:-78966137 | None:intergenic |
| TTGTGGAGAAGGAGAGGTGG+AGG | 0.466260 | 1.4:+78967263 | MS.gene41380:CDS |
| TATAAGGTACCTTCCTCTGT+TGG | 0.479890 | 1.4:-78966822 | None:intergenic |
| ATTGCGTACTTTCTGGTACA+AGG | 0.480220 | 1.4:-78966499 | None:intergenic |
| TCTGCTATGAGTTCTTTCTC+TGG | 0.493691 | 1.4:+78966608 | MS.gene41380:CDS |
| CTAAAACACCCTAATGATGA+AGG | 0.494035 | 1.4:+78966872 | MS.gene41380:CDS |
| ACTGTTTCTGGGTTTGGTTG+TGG | 0.497829 | 1.4:-78965475 | None:intergenic |
| AACAATATATCTCTTAGCTA+TGG | 0.500270 | 1.4:+78967022 | MS.gene41380:CDS |
| CTATGAGTTCTTTCTCTGGA+AGG | 0.500985 | 1.4:+78966612 | MS.gene41380:CDS |
| GGACAGCAACCTTGAGAGAC+CGG | 0.501539 | 1.4:-78965408 | None:intergenic |
| TATTGTTGTGGAGATTGAAA+TGG | 0.511166 | 1.4:-78965795 | None:intergenic |
| AGATAAGTACTCAGAAGAGA+AGG | 0.512225 | 1.4:+78967069 | MS.gene41380:CDS |
| GAAGTGATGGAAGAGATTCA+TGG | 0.514570 | 1.4:-78965566 | None:intergenic |
| TCTTCGGGTAATAATCAAGT+AGG | 0.514809 | 1.4:+78966542 | MS.gene41380:CDS |
| AGAGTCCTACAGACAAAAGA+AGG | 0.521500 | 1.4:+78967094 | MS.gene41380:CDS |
| TGTATTTCCACTTCATTCAA+GGG | 0.524789 | 1.4:+78966226 | MS.gene41380:CDS |
| CCTGTTACCCTTGAATGAAG+TGG | 0.538045 | 1.4:-78966233 | None:intergenic |
| ATTGGTTTAAGAAAGAAAGA+TGG | 0.538058 | 1.4:-78965674 | None:intergenic |
| ATGATGTTGATGCAAAGGTT+TGG | 0.538497 | 1.4:-78965512 | None:intergenic |
| TTCAATGTTTATGATTGGAA+AGG | 0.543000 | 1.4:+78966956 | MS.gene41380:CDS |
| TTGTAGGAAATACTAAAGAA+GGG | 0.546032 | 1.4:-78965828 | None:intergenic |
| CAATCTTGTCTTGATTACAT+TGG | 0.546667 | 1.4:+78965693 | MS.gene41380:CDS |
| GACAATTCCTGCACAAGTGG+AGG | 0.546718 | 1.4:-78966474 | None:intergenic |
| AGATGATTTGGGAGTGACAG+TGG | 0.550613 | 1.4:+78967156 | MS.gene41380:CDS |
| GCTGTTCTCATCCAAAGATA+AGG | 0.552898 | 1.4:-78966266 | None:intergenic |
| TTCTCAGTCTCAGAGAATTG+TGG | 0.553672 | 1.4:-78965621 | None:intergenic |
| GTTGACAATTCCTGCACAAG+TGG | 0.555713 | 1.4:-78966477 | None:intergenic |
| TGAAATGGTGTTGAAGTTGA+AGG | 0.555820 | 1.4:-78965780 | None:intergenic |
| GAACAGCACAAGAGAATGGT+TGG | 0.558173 | 1.4:+78966282 | MS.gene41380:CDS |
| TGAGGATGATGTTGATGCAA+AGG | 0.559727 | 1.4:-78965517 | None:intergenic |
| CTACAGACAAAAGAAGGAAG+AGG | 0.560836 | 1.4:+78967100 | MS.gene41380:CDS |
| CTATGTCAGCTGAATTCTCA+TGG | 0.562090 | 1.4:+78966114 | MS.gene41380:CDS |
| GATTTGATGAACAATAAGGT+TGG | 0.562625 | 1.4:-78965429 | None:intergenic |
| ATGAGAACAGCACAAGAGAA+TGG | 0.563673 | 1.4:+78966278 | MS.gene41380:CDS |
| CAGCTGAATTCTCATGGCCA+AGG | 0.567394 | 1.4:+78966120 | MS.gene41380:CDS |
| AGAGCTTTAACATTGAATGC+AGG | 0.572458 | 1.4:+78966759 | MS.gene41380:CDS |
| GGACCATAAATCTTGACTAA+GGG | 0.573713 | 1.4:-78966899 | None:intergenic |
| TAAAACACCCTAATGATGAA+GGG | 0.576153 | 1.4:+78966873 | MS.gene41380:CDS |
| TAAACATTGAAAGCTAAAGC+AGG | 0.579333 | 1.4:-78966944 | None:intergenic |
| CACTGATAACCAACAGAGGA+AGG | 0.580267 | 1.4:+78966813 | MS.gene41380:CDS |
| ATGTTGATGCAAAGGTTTGG+CGG | 0.584928 | 1.4:-78965509 | None:intergenic |
| AGACTTTATTGCTTCATGGA+GGG | 0.585375 | 1.4:+78965865 | MS.gene41380:CDS |
| ATGCAGGTGTTTGGATCAAG+TGG | 0.586464 | 1.4:+78966775 | MS.gene41380:CDS |
| TCTCCACAAAATCTGCATCA+AGG | 0.587719 | 1.4:-78967249 | None:intergenic |
| AGGAAGAGGAGAGAAAGTAA+TGG | 0.588435 | 1.4:+78967114 | MS.gene41380:CDS |
| GTGAATGATTCATGAAGTGA+TGG | 0.589772 | 1.4:-78965579 | None:intergenic |
| GGTATATCACAATATTGTTG+TGG | 0.596028 | 1.4:-78965807 | None:intergenic |
| GATAAGTACTCAGAAGAGAA+GGG | 0.597070 | 1.4:+78967070 | MS.gene41380:CDS |
| CCAAAGATAAGATTTGATAG+AGG | 0.603821 | 1.4:+78966920 | MS.gene41380:CDS |
| ACAAGTGGAGGATTCAAGAA+TGG | 0.606389 | 1.4:-78966462 | None:intergenic |
| AAGACTTTATTGCTTCATGG+AGG | 0.616374 | 1.4:+78965864 | MS.gene41380:CDS |
| TGACTATGAGAGTGAAGCAG+TGG | 0.616607 | 1.4:+78966052 | MS.gene41380:CDS |
| AAAAGGCAACCAAAACCAGA+TGG | 0.623470 | 1.4:-78966419 | None:intergenic |
| AAAACACCCTAATGATGAAG+GGG | 0.635176 | 1.4:+78966874 | MS.gene41380:CDS |
| CAGAATTTGAGTCTGCAATG+AGG | 0.651477 | 1.4:+78965895 | MS.gene41380:CDS |
| TGCATTAGGACCAAAAGACA+TGG | 0.657489 | 1.4:+78966325 | MS.gene41380:CDS |
| ACTAGGTTCTGAAACTATGG+AGG | 0.664206 | 1.4:+78966682 | MS.gene41380:CDS |
| GCAGATCCAAAAGTTCCACT+AGG | 0.665397 | 1.4:+78966665 | MS.gene41380:CDS |
| TCCATAGTTTCAGAACCTAG+TGG | 0.670278 | 1.4:-78966680 | None:intergenic |
| TTGACACTGATAACCAACAG+AGG | 0.715180 | 1.4:+78966809 | MS.gene41380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAAGATAAAGAAGAAAAA+TGG | + | chr1.4:78966197-78966216 | MS.gene41380:CDS | 20.0% |
| !!! | TTCTGAAAATGATTATTTCA+TGG | + | chr1.4:78965944-78965963 | MS.gene41380:CDS | 20.0% |
| ! | AAAGAAAGATAGAGATGATT+TGG | + | chr1.4:78967144-78967163 | MS.gene41380:CDS | 25.0% |
| ! | AACAATATATCTCTTAGCTA+TGG | + | chr1.4:78967022-78967041 | MS.gene41380:CDS | 25.0% |
| ! | AACAATCATCTTCTTCAATT+TGG | - | chr1.4:78966162-78966181 | None:intergenic | 25.0% |
| ! | AAGAAAGATAGAGATGATTT+GGG | + | chr1.4:78967145-78967164 | MS.gene41380:CDS | 25.0% |
| ! | AATGAACAAATGAGAAAATC+TGG | - | chr1.4:78966377-78966396 | None:intergenic | 25.0% |
| ! | AGAAAGATGGTAATATTCTT+TGG | - | chr1.4:78965664-78965683 | None:intergenic | 25.0% |
| ! | AGTTTACAAGCCAATTTATA+AGG | - | chr1.4:78966841-78966860 | None:intergenic | 25.0% |
| ! | ATTAGTACTTCAGAAAAAAG+AGG | + | chr1.4:78966083-78966102 | MS.gene41380:CDS | 25.0% |
| ! | TAGAAGATTTCTTGACAAAA+AGG | - | chr1.4:78966439-78966458 | None:intergenic | 25.0% |
| ! | TAGCTTTCAATGTTTATGAT+TGG | + | chr1.4:78966951-78966970 | MS.gene41380:CDS | 25.0% |
| ! | TCTATCAGAAGATTATTCTT+CGG | + | chr1.4:78966526-78966545 | MS.gene41380:CDS | 25.0% |
| ! | TGATGATTTGATGAACAATA+AGG | - | chr1.4:78965436-78965455 | None:intergenic | 25.0% |
| ! | TTCAATGTTTATGATTGGAA+AGG | + | chr1.4:78966956-78966975 | MS.gene41380:CDS | 25.0% |
| ! | TTGAAGATGTGTATAAACTA+TGG | + | chr1.4:78967209-78967228 | MS.gene41380:CDS | 25.0% |
| ! | TTGTAGGAAATACTAAAGAA+GGG | - | chr1.4:78965831-78965850 | None:intergenic | 25.0% |
| ! | TTTAGTATTTCCTACAAAAC+TGG | + | chr1.4:78965834-78965853 | MS.gene41380:CDS | 25.0% |
| ! | TTTGTAGGAAATACTAAAGA+AGG | - | chr1.4:78965832-78965851 | None:intergenic | 25.0% |
| !! | ATTGGTTTAAGAAAGAAAGA+TGG | - | chr1.4:78965677-78965696 | None:intergenic | 25.0% |
| !! | TGTTCATTCTACAAAGTTTT+TGG | + | chr1.4:78966389-78966408 | MS.gene41380:CDS | 25.0% |
| AGATTAAATTGCGTACTTTC+TGG | - | chr1.4:78966509-78966528 | None:intergenic | 30.0% | |
| AGCTTCTTACAAGATTGAAA+TGG | - | chr1.4:78966020-78966039 | None:intergenic | 30.0% | |
| ATGGATTTCTGCATCATATT+TGG | + | chr1.4:78967041-78967060 | MS.gene41380:CDS | 30.0% | |
| CAATCTTGTCTTGATTACAT+TGG | + | chr1.4:78965693-78965712 | MS.gene41380:CDS | 30.0% | |
| CAATGTAATCAAGACAAGAT+TGG | - | chr1.4:78965695-78965714 | None:intergenic | 30.0% | |
| CCAAAGATAAGATTTGATAG+AGG | + | chr1.4:78966920-78966939 | MS.gene41380:CDS | 30.0% | |
| CCTCTATCAAATCTTATCTT+TGG | - | chr1.4:78966923-78966942 | None:intergenic | 30.0% | |
| CTATCAGAAGATTATTCTTC+GGG | + | chr1.4:78966527-78966546 | MS.gene41380:CDS | 30.0% | |
| GATTTGATGAACAATAAGGT+TGG | - | chr1.4:78965432-78965451 | None:intergenic | 30.0% | |
| GGTATATCACAATATTGTTG+TGG | - | chr1.4:78965810-78965829 | None:intergenic | 30.0% | |
| TAAAACACCCTAATGATGAA+GGG | + | chr1.4:78966873-78966892 | MS.gene41380:CDS | 30.0% | |
| TAAACATTGAAAGCTAAAGC+AGG | - | chr1.4:78966947-78966966 | None:intergenic | 30.0% | |
| TATTGTTGTGGAGATTGAAA+TGG | - | chr1.4:78965798-78965817 | None:intergenic | 30.0% | |
| TGTATTTCCACTTCATTCAA+GGG | + | chr1.4:78966226-78966245 | MS.gene41380:CDS | 30.0% | |
| TGTCTTGATTACATTGGTAT+TGG | + | chr1.4:78965699-78965718 | MS.gene41380:CDS | 30.0% | |
| TTGAAGACTTTATTGCTTCA+TGG | + | chr1.4:78965861-78965880 | MS.gene41380:CDS | 30.0% | |
| TTGTATTTCCACTTCATTCA+AGG | + | chr1.4:78966225-78966244 | MS.gene41380:CDS | 30.0% | |
| ! | AGATTCATGGTTTGTGAATT+TGG | - | chr1.4:78965556-78965575 | None:intergenic | 30.0% |
| ! | GCTTCAACTTTTACAAATTG+TGG | - | chr1.4:78966647-78966666 | None:intergenic | 30.0% |
| !! | ATGTTGTTGACGTTGATAAT+AGG | - | chr1.4:78965733-78965752 | None:intergenic | 30.0% |
| !! | CTTCAAATTTCCAGTTTTGT+AGG | - | chr1.4:78965847-78965866 | None:intergenic | 30.0% |
| !! | TTTGGTGTTTGGAAAAATTG+AGG | - | chr1.4:78965538-78965557 | None:intergenic | 30.0% |
| AAAACACCCTAATGATGAAG+GGG | + | chr1.4:78966874-78966893 | MS.gene41380:CDS | 35.0% | |
| AAGACTTTATTGCTTCATGG+AGG | + | chr1.4:78965864-78965883 | MS.gene41380:CDS | 35.0% | |
| AGACTTTATTGCTTCATGGA+GGG | + | chr1.4:78965865-78965884 | MS.gene41380:CDS | 35.0% | |
| AGATAAGTACTCAGAAGAGA+AGG | + | chr1.4:78967069-78967088 | MS.gene41380:CDS | 35.0% | |
| ATGATGTTGATGCAAAGGTT+TGG | - | chr1.4:78965515-78965534 | None:intergenic | 35.0% | |
| CTAAAACACCCTAATGATGA+AGG | + | chr1.4:78966872-78966891 | MS.gene41380:CDS | 35.0% | |
| GACAACACTTAACCAAAAAC+CGG | + | chr1.4:78965389-78965408 | MS.gene41380:CDS | 35.0% | |
| GAGCAAGATATCCTTATCTT+TGG | + | chr1.4:78966255-78966274 | MS.gene41380:CDS | 35.0% | |
| GATAAGTACTCAGAAGAGAA+GGG | + | chr1.4:78967070-78967089 | MS.gene41380:CDS | 35.0% | |
| GCAAGTTTCTGTACTAAAAC+AGG | - | chr1.4:78966995-78967014 | None:intergenic | 35.0% | |
| GGACCATAAATCTTGACTAA+GGG | - | chr1.4:78966902-78966921 | None:intergenic | 35.0% | |
| GGTAATAATCAAGTAGGAAC+TGG | + | chr1.4:78966548-78966567 | MS.gene41380:CDS | 35.0% | |
| GTGAATGATTCATGAAGTGA+TGG | - | chr1.4:78965582-78965601 | None:intergenic | 35.0% | |
| GTTCCCTTAGTCAAGATTTA+TGG | + | chr1.4:78966896-78966915 | MS.gene41380:CDS | 35.0% | |
| GTTGATTGAATTCTAAGCCT+TGG | - | chr1.4:78966140-78966159 | None:intergenic | 35.0% | |
| TAACATTGAATGCAGGTGTT+TGG | + | chr1.4:78966766-78966785 | MS.gene41380:CDS | 35.0% | |
| TCCTGCTCTTGTAAAATAGA+TGG | - | chr1.4:78966584-78966603 | None:intergenic | 35.0% | |
| TCTTCGGGTAATAATCAAGT+AGG | + | chr1.4:78966542-78966561 | MS.gene41380:CDS | 35.0% | |
| TGAAACTATGGAGGCAAAAA+GGG | + | chr1.4:78966691-78966710 | MS.gene41380:CDS | 35.0% | |
| TGGACCATAAATCTTGACTA+AGG | - | chr1.4:78966903-78966922 | None:intergenic | 35.0% | |
| TTGATTGATGCTTGTGCATT+AGG | + | chr1.4:78966311-78966330 | MS.gene41380:CDS | 35.0% | |
| ! | AACTATGGACTTTTGTTGCT+AGG | + | chr1.4:78967224-78967243 | MS.gene41380:CDS | 35.0% |
| ! | ACCATCTATTTTACAAGAGC+AGG | + | chr1.4:78966580-78966599 | MS.gene41380:CDS | 35.0% |
| ! | AGAGCTTTAACATTGAATGC+AGG | + | chr1.4:78966759-78966778 | MS.gene41380:CDS | 35.0% |
| ! | CATTGTGAAGAACTGTTTCT+GGG | - | chr1.4:78965489-78965508 | None:intergenic | 35.0% |
| ! | TGAAATGGTGTTGAAGTTGA+AGG | - | chr1.4:78965783-78965802 | None:intergenic | 35.0% |
| !! | GAGGAAGGTACCTTATAAAT+TGG | + | chr1.4:78966828-78966847 | MS.gene41380:CDS | 35.0% |
| !! | GTTTTTGGAGAAAATCCATC+TGG | + | chr1.4:78966404-78966423 | MS.gene41380:CDS | 35.0% |
| !! | TGGTTTGTGAATTTGGTGTT+TGG | - | chr1.4:78965549-78965568 | None:intergenic | 35.0% |
| AAAAGGCAACCAAAACCAGA+TGG | - | chr1.4:78966422-78966441 | None:intergenic | 40.0% | |
| ACAAGTGGAGGATTCAAGAA+TGG | - | chr1.4:78966465-78966484 | None:intergenic | 40.0% | |
| AGAGTCCTACAGACAAAAGA+AGG | + | chr1.4:78967094-78967113 | MS.gene41380:CDS | 40.0% | |
| AGGAAGAGGAGAGAAAGTAA+TGG | + | chr1.4:78967114-78967133 | MS.gene41380:CDS | 40.0% | |
| ATGAGAACAGCACAAGAGAA+TGG | + | chr1.4:78966278-78966297 | MS.gene41380:CDS | 40.0% | |
| ATGTTGATGCAAAGGTTTGG+CGG | - | chr1.4:78965512-78965531 | None:intergenic | 40.0% | |
| CAGAATTTGAGTCTGCAATG+AGG | + | chr1.4:78965895-78965914 | MS.gene41380:CDS | 40.0% | |
| CTACAGACAAAAGAAGGAAG+AGG | + | chr1.4:78967100-78967119 | MS.gene41380:CDS | 40.0% | |
| CTATGAGTTCTTTCTCTGGA+AGG | + | chr1.4:78966612-78966631 | MS.gene41380:CDS | 40.0% | |
| CTATGTCAGCTGAATTCTCA+TGG | + | chr1.4:78966114-78966133 | MS.gene41380:CDS | 40.0% | |
| CTGAAACTATGGAGGCAAAA+AGG | + | chr1.4:78966690-78966709 | MS.gene41380:CDS | 40.0% | |
| GCATTGTGAAGAACTGTTTC+TGG | - | chr1.4:78965490-78965509 | None:intergenic | 40.0% | |
| GCTGTTCTCATCCAAAGATA+AGG | - | chr1.4:78966269-78966288 | None:intergenic | 40.0% | |
| TCCATAGTTTCAGAACCTAG+TGG | - | chr1.4:78966683-78966702 | None:intergenic | 40.0% | |
| TCTCCACAAAATCTGCATCA+AGG | - | chr1.4:78967252-78967271 | None:intergenic | 40.0% | |
| TCTGCTATGAGTTCTTTCTC+TGG | + | chr1.4:78966608-78966627 | MS.gene41380:CDS | 40.0% | |
| TGAGGATGATGTTGATGCAA+AGG | - | chr1.4:78965520-78965539 | None:intergenic | 40.0% | |
| TGCATTAGGACCAAAAGACA+TGG | + | chr1.4:78966325-78966344 | MS.gene41380:CDS | 40.0% | |
| TTCTCAGTCTCAGAGAATTG+TGG | - | chr1.4:78965624-78965643 | None:intergenic | 40.0% | |
| TTGACACTGATAACCAACAG+AGG | + | chr1.4:78966809-78966828 | MS.gene41380:CDS | 40.0% | |
| TTGAGTCTGCAATGAGGAAA+AGG | + | chr1.4:78965901-78965920 | MS.gene41380:CDS | 40.0% | |
| ! | ATTGCGTACTTTCTGGTACA+AGG | - | chr1.4:78966502-78966521 | None:intergenic | 40.0% |
| ! | CCAAAACTGTCCATGTCTTT+TGG | - | chr1.4:78966338-78966357 | None:intergenic | 40.0% |
| ! | CCAAAAGACATGGACAGTTT+TGG | + | chr1.4:78966335-78966354 | MS.gene41380:CDS | 40.0% |
| ! | CTCTTCCTTCTTTTGTCTGT+AGG | - | chr1.4:78967102-78967121 | None:intergenic | 40.0% |
| ! | GAAGTGATGGAAGAGATTCA+TGG | - | chr1.4:78965569-78965588 | None:intergenic | 40.0% |
| ! | GTTCCTTGATGCAGATTTTG+TGG | + | chr1.4:78967246-78967265 | MS.gene41380:CDS | 40.0% |
| ! | TATAAGGTACCTTCCTCTGT+TGG | - | chr1.4:78966825-78966844 | None:intergenic | 40.0% |
| ! | TCAGAACCTAGTGGAACTTT+TGG | - | chr1.4:78966674-78966693 | None:intergenic | 40.0% |
| ! | TGATGCAGATTTTGTGGAGA+AGG | + | chr1.4:78967252-78967271 | MS.gene41380:CDS | 40.0% |
| ! | TTGGATCAAGTGGACTCTTT+AGG | + | chr1.4:78966785-78966804 | MS.gene41380:CDS | 40.0% |
| !! | ACTAGGTTCTGAAACTATGG+AGG | + | chr1.4:78966682-78966701 | MS.gene41380:CDS | 40.0% |
| !! | GGAGAAAATCCATCTGGTTT+TGG | + | chr1.4:78966410-78966429 | MS.gene41380:CDS | 40.0% |
| !! | TCCACTAGGTTCTGAAACTA+TGG | + | chr1.4:78966679-78966698 | MS.gene41380:CDS | 40.0% |
| !! | TGAAGAACTGTTTCTGGGTT+TGG | - | chr1.4:78965484-78965503 | None:intergenic | 40.0% |
| AACCAAAAACCGGTCTCTCA+AGG | + | chr1.4:78965399-78965418 | MS.gene41380:CDS | 45.0% | |
| AAGGGAACCCCTTCATCATT+AGG | - | chr1.4:78966884-78966903 | None:intergenic | 45.0% | |
| AGGGAACCCCTTCATCATTA+GGG | - | chr1.4:78966883-78966902 | None:intergenic | 45.0% | |
| ATGCAGGTGTTTGGATCAAG+TGG | + | chr1.4:78966775-78966794 | MS.gene41380:CDS | 45.0% | |
| CACTGATAACCAACAGAGGA+AGG | + | chr1.4:78966813-78966832 | MS.gene41380:CDS | 45.0% | |
| CCACTTCATTCAAGGGTAAC+AGG | + | chr1.4:78966233-78966252 | MS.gene41380:CDS | 45.0% | |
| CCTGTTACCCTTGAATGAAG+TGG | - | chr1.4:78966236-78966255 | None:intergenic | 45.0% | |
| GAACAGCACAAGAGAATGGT+TGG | + | chr1.4:78966282-78966301 | MS.gene41380:CDS | 45.0% | |
| GCAGATCCAAAAGTTCCACT+AGG | + | chr1.4:78966665-78966684 | MS.gene41380:CDS | 45.0% | |
| GTTGACAATTCCTGCACAAG+TGG | - | chr1.4:78966480-78966499 | None:intergenic | 45.0% | |
| TACTGCTGCATTGAGCTTCT+TGG | + | chr1.4:78967180-78967199 | MS.gene41380:CDS | 45.0% | |
| TGACTATGAGAGTGAAGCAG+TGG | + | chr1.4:78966052-78966071 | MS.gene41380:CDS | 45.0% | |
| ! | AGATGATTTGGGAGTGACAG+TGG | + | chr1.4:78967156-78967175 | MS.gene41380:CDS | 45.0% |
| ! | ATTTTGTGGAGAAGGAGAGG+TGG | + | chr1.4:78967260-78967279 | MS.gene41380:CDS | 45.0% |
| ! | CAGATTTTGTGGAGAAGGAG+AGG | + | chr1.4:78967257-78967276 | MS.gene41380:CDS | 45.0% |
| !! | AACCTTGAGAGACCGGTTTT+TGG | - | chr1.4:78965404-78965423 | None:intergenic | 45.0% |
| !! | ACTGTTTCTGGGTTTGGTTG+TGG | - | chr1.4:78965478-78965497 | None:intergenic | 45.0% |
| CAGCTGAATTCTCATGGCCA+AGG | + | chr1.4:78966120-78966139 | MS.gene41380:CDS | 50.0% | |
| CTTGAATCCTCCACTTGTGC+AGG | + | chr1.4:78966467-78966486 | MS.gene41380:CDS | 50.0% | |
| GACAATTCCTGCACAAGTGG+AGG | - | chr1.4:78966477-78966496 | None:intergenic | 50.0% | |
| GGACAGCAACCTTGAGAGAC+CGG | - | chr1.4:78965411-78965430 | None:intergenic | 55.0% | |
| TTGTGGAGAAGGAGAGGTGG+AGG | + | chr1.4:78967263-78967282 | MS.gene41380:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 78965384 | 78967321 | 78965384 | ID=MS.gene41380 |
| chr1.4 | mRNA | 78965384 | 78967321 | 78965384 | ID=MS.gene41380.t1;Parent=MS.gene41380 |
| chr1.4 | exon | 78965384 | 78967321 | 78965384 | ID=MS.gene41380.t1.exon1;Parent=MS.gene41380.t1 |
| chr1.4 | CDS | 78965384 | 78967321 | 78965384 | ID=cds.MS.gene41380.t1;Parent=MS.gene41380.t1 |
| Gene Sequence |
| Protein sequence |
