Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44595.t1 | XP_013452335.1 | 84.9 | 365 | 43 | 4 | 1 | 364 | 1 | 354 | 2.70E-161 | 578.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44595.t1 | Q8LG95 | 68.7 | 233 | 72 | 1 | 65 | 296 | 11 | 243 | 2.0e-88 | 327.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44595.t1 | A0A072UBE9 | 84.9 | 365 | 43 | 4 | 1 | 364 | 1 | 354 | 2.0e-161 | 578.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene44595 | 0.809343 | 1.98E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene44595 | 0.804507 | 2.09E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene44595 | 0.815409 | 9.38E-52 | -1.69E-46 |
| MS.gene049910 | MS.gene44595 | 0.822101 | 2.84E-53 | -1.69E-46 |
| MS.gene050226 | MS.gene44595 | 0.822916 | 1.83E-53 | -1.69E-46 |
| MS.gene050227 | MS.gene44595 | 0.809699 | 1.66E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene44595 | 0.815964 | 7.06E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene44595 | 0.827164 | 1.82E-54 | -1.69E-46 |
| MS.gene050558 | MS.gene44595 | 0.802183 | 6.33E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene44595 | 0.83238 | 9.77E-56 | -1.69E-46 |
| MS.gene050820 | MS.gene44595 | 0.827286 | 1.70E-54 | -1.69E-46 |
| MS.gene05119 | MS.gene44595 | -0.802692 | 4.97E-49 | -1.69E-46 |
| MS.gene051663 | MS.gene44595 | 0.801858 | 7.38E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene44595 | 0.820791 | 5.69E-53 | -1.69E-46 |
| MS.gene051983 | MS.gene44595 | 0.818771 | 1.65E-52 | -1.69E-46 |
| MS.gene052188 | MS.gene44595 | 0.814937 | 1.19E-51 | -1.69E-46 |
| MS.gene052253 | MS.gene44595 | 0.823895 | 1.08E-53 | -1.69E-46 |
| MS.gene052340 | MS.gene44595 | 0.811592 | 6.48E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene44595 | 0.864238 | 1.40E-64 | -1.69E-46 |
| MS.gene054473 | MS.gene44595 | 0.805508 | 1.29E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene44595 | 0.841857 | 3.69E-58 | -1.69E-46 |
| MS.gene055422 | MS.gene44595 | 0.82552 | 4.48E-54 | -1.69E-46 |
| MS.gene055428 | MS.gene44595 | 0.801338 | 9.44E-49 | -1.69E-46 |
| MS.gene056723 | MS.gene44595 | 0.80801 | 3.82E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene44595 | 0.8295 | 4.97E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene44595 | 0.841498 | 4.58E-58 | -1.69E-46 |
| MS.gene057245 | MS.gene44595 | 0.814011 | 1.91E-51 | -1.69E-46 |
| MS.gene057448 | MS.gene44595 | 0.812138 | 4.93E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene44595 | 0.810884 | 9.23E-51 | -1.69E-46 |
| MS.gene057469 | MS.gene44595 | 0.805234 | 1.47E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene44595 | 0.836246 | 1.05E-56 | -1.69E-46 |
| MS.gene058142 | MS.gene44595 | 0.819719 | 1.00E-52 | -1.69E-46 |
| MS.gene058710 | MS.gene44595 | 0.804951 | 1.69E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene44595 | 0.814253 | 1.69E-51 | -1.69E-46 |
| MS.gene059128 | MS.gene44595 | 0.807904 | 4.02E-50 | -1.69E-46 |
| MS.gene059285 | MS.gene44595 | 0.836422 | 9.45E-57 | -1.69E-46 |
| MS.gene059420 | MS.gene44595 | 0.812826 | 3.49E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene44595 | 0.800994 | 1.11E-48 | -1.69E-46 |
| MS.gene05984 | MS.gene44595 | 0.847339 | 1.23E-59 | -1.69E-46 |
| MS.gene061049 | MS.gene44595 | 0.810852 | 9.38E-51 | -1.69E-46 |
| MS.gene061114 | MS.gene44595 | -0.80713 | 5.87E-50 | -1.69E-46 |
| MS.gene061159 | MS.gene44595 | 0.817127 | 3.87E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44595.t1 | MTR_6g053090 | 84.932 | 365 | 43 | 4 | 1 | 364 | 1 | 354 | 0.0 | 599 |
| MS.gene44595.t1 | MTR_3g024400 | 46.500 | 200 | 106 | 1 | 98 | 297 | 76 | 274 | 4.58e-55 | 182 |
| MS.gene44595.t1 | MTR_5g012120 | 39.744 | 156 | 93 | 1 | 122 | 276 | 4 | 159 | 1.16e-35 | 129 |
| MS.gene44595.t1 | MTR_5g012120 | 37.952 | 166 | 100 | 2 | 114 | 276 | 71 | 236 | 1.98e-35 | 130 |
| MS.gene44595.t1 | MTR_3g024400 | 45.455 | 132 | 71 | 1 | 166 | 297 | 96 | 226 | 3.19e-32 | 121 |
| MS.gene44595.t1 | MTR_6g052450 | 50.893 | 112 | 40 | 3 | 57 | 160 | 1 | 105 | 3.28e-21 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44595.t1 | AT4G21190 | 68.670 | 233 | 72 | 1 | 65 | 296 | 11 | 243 | 2.89e-116 | 340 |
| MS.gene44595.t1 | AT4G18975 | 47.208 | 197 | 103 | 1 | 98 | 294 | 58 | 253 | 1.37e-58 | 191 |
| MS.gene44595.t1 | AT4G18975 | 47.208 | 197 | 103 | 1 | 98 | 294 | 85 | 280 | 1.38e-58 | 191 |
| MS.gene44595.t1 | AT4G18975 | 47.208 | 197 | 103 | 1 | 98 | 294 | 85 | 280 | 1.38e-58 | 191 |
| MS.gene44595.t1 | AT4G18975 | 47.208 | 197 | 103 | 1 | 98 | 294 | 85 | 280 | 1.38e-58 | 191 |
| MS.gene44595.t1 | AT1G04590 | 38.462 | 182 | 111 | 1 | 96 | 276 | 131 | 312 | 1.13e-36 | 137 |
| MS.gene44595.t1 | AT1G04590 | 38.378 | 185 | 110 | 2 | 96 | 276 | 131 | 315 | 4.28e-35 | 132 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43118 | gene | 112496 | 122225 | 112496 | ID=MS.gene44595 |
| 43118 | mRNA | 112496 | 122225 | 112496 | ID=MS.gene44595.t1;Parent=MS.gene44595 |
| 43118 | exon | 112496 | 112744 | 112496 | ID=MS.gene44595.t1.exon1;Parent=MS.gene44595.t1 |
| 43118 | CDS | 112496 | 112744 | 112496 | ID=cds.MS.gene44595.t1;Parent=MS.gene44595.t1 |
| 43118 | exon | 118697 | 118798 | 118697 | ID=MS.gene44595.t1.exon2;Parent=MS.gene44595.t1 |
| 43118 | CDS | 118697 | 118798 | 118697 | ID=cds.MS.gene44595.t1;Parent=MS.gene44595.t1 |
| 43118 | exon | 120116 | 120259 | 120116 | ID=MS.gene44595.t1.exon3;Parent=MS.gene44595.t1 |
| 43118 | CDS | 120116 | 120259 | 120116 | ID=cds.MS.gene44595.t1;Parent=MS.gene44595.t1 |
| 43118 | exon | 120461 | 120673 | 120461 | ID=MS.gene44595.t1.exon4;Parent=MS.gene44595.t1 |
| 43118 | CDS | 120461 | 120673 | 120461 | ID=cds.MS.gene44595.t1;Parent=MS.gene44595.t1 |
| 43118 | exon | 121839 | 122225 | 121839 | ID=MS.gene44595.t1.exon5;Parent=MS.gene44595.t1 |
| 43118 | CDS | 121839 | 122225 | 121839 | ID=cds.MS.gene44595.t1;Parent=MS.gene44595.t1 |
| Gene Sequence |
| Protein sequence |
