AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46786

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46786.t1 MTR_3g028590 98.715 389 5 0 1 389 5 393 0.0 786
MS.gene46786.t1 MTR_3g028590 98.715 389 5 0 1 389 1 389 0.0 785
MS.gene46786.t1 MTR_8g036880 81.795 390 45 4 1 389 35 399 0.0 607
MS.gene46786.t1 MTR_4g078545 75.128 390 75 3 1 388 1 370 0.0 547
MS.gene46786.t1 MTR_2g098070 74.444 360 83 4 35 389 7 362 6.60e-178 499
MS.gene46786.t1 MTR_8g107270 59.649 285 115 0 92 376 17 301 4.66e-128 371
MS.gene46786.t1 MTR_3g104500 39.527 296 168 6 87 376 28 318 1.66e-67 216
MS.gene46786.t1 MTR_3g104600 31.715 309 177 9 89 376 31 326 1.69e-35 133
MS.gene46786.t1 MTR_6g091800 28.713 303 187 7 89 376 15 303 1.12e-32 125
MS.gene46786.t1 MTR_6g091800 27.245 323 190 7 89 376 15 327 8.21e-29 115
MS.gene46786.t1 MTR_6g091800 28.839 267 164 6 89 344 15 266 1.80e-28 112
MS.gene46786.t1 MTR_5g009500 29.022 317 172 10 81 363 24 321 3.03e-28 114
MS.gene46786.t1 MTR_6g091800 30.328 244 153 4 89 325 15 248 1.26e-27 110
MS.gene46786.t1 MTR_8g027975 29.195 298 174 9 92 363 50 336 2.49e-27 111
MS.gene46786.t1 MTR_8g027975 26.108 203 131 5 96 285 157 353 7.48e-11 63.5
MS.gene46786.t1 MTR_6g091800 27.273 308 180 6 89 362 15 312 3.43e-27 110
MS.gene46786.t1 MTR_7g064980 28.904 301 195 8 90 379 22 314 7.14e-27 109
MS.gene46786.t1 MTR_1g012030 30.314 287 162 11 93 368 139 398 1.94e-26 110
MS.gene46786.t1 MTR_1g012030 27.143 210 132 6 81 286 219 411 3.91e-14 73.9
MS.gene46786.t1 MTR_3g107510 28.881 277 173 8 96 368 125 381 7.69e-26 108
MS.gene46786.t1 MTR_3g107510 27.315 216 136 6 81 292 202 400 1.61e-15 77.8
MS.gene46786.t1 MTR_8g096050 29.617 287 168 9 93 368 227 490 2.20e-25 107
MS.gene46786.t1 MTR_7g084400 30.345 290 159 8 93 368 213 473 5.29e-25 106
MS.gene46786.t1 MTR_7g068340 29.329 283 169 8 104 379 98 356 1.08e-24 104
MS.gene46786.t1 MTR_6g091800 27.148 291 162 7 89 344 15 290 1.36e-24 102
MS.gene46786.t1 MTR_4g119770 26.444 329 195 8 83 375 4 321 2.95e-24 102
MS.gene46786.t1 MTR_6g091800 28.358 268 151 5 89 325 15 272 6.46e-24 100
MS.gene46786.t1 MTR_4g114320 27.465 284 174 7 93 368 123 382 8.10e-24 102
MS.gene46786.t1 MTR_4g114320 27.751 209 127 6 83 286 206 395 3.11e-14 73.9
MS.gene46786.t1 MTR_5g081150 26.144 306 209 7 83 379 24 321 1.11e-23 100
MS.gene46786.t1 MTR_3g098460 28.053 303 181 11 77 368 192 468 4.32e-23 100
MS.gene46786.t1 MTR_2g436930 27.358 318 186 9 90 373 12 318 1.15e-22 97.8
MS.gene46786.t1 MTR_6g034145 28.340 247 163 5 141 376 6 249 1.26e-22 96.3
MS.gene46786.t1 MTR_6g091800 31.148 183 110 3 89 264 15 188 1.49e-22 94.4
MS.gene46786.t1 MTR_4g016730 29.191 346 203 13 40 379 46 355 2.07e-22 97.8
MS.gene46786.t1 MTR_6g092210 28.216 241 165 5 141 376 6 243 4.13e-22 94.7
MS.gene46786.t1 MTR_4g119770 26.592 267 160 6 83 324 4 259 2.57e-19 87.0
MS.gene46786.t1 MTR_6g091800 28.502 207 108 4 89 264 15 212 1.00e-18 84.3
MS.gene46786.t1 MTR_3g074490 27.946 297 166 12 96 368 119 391 1.55e-17 84.0
MS.gene46786.t1 MTR_4g005330 24.000 300 186 7 90 368 63 341 3.00e-17 82.8
MS.gene46786.t1 MTR_1g090330 22.180 266 195 4 93 354 14 271 5.58e-17 81.3
MS.gene46786.t1 MTR_1g090330 28.571 168 112 4 94 260 112 272 8.22e-13 68.9
MS.gene46786.t1 MTR_2g102010 25.874 286 195 6 92 373 16 288 9.49e-17 80.5
MS.gene46786.t1 MTR_4g005330 23.810 294 182 7 96 368 3 275 3.68e-16 78.6
MS.gene46786.t1 MTR_5g030530 26.502 283 187 9 92 363 7 279 8.45e-16 77.8
MS.gene46786.t1 MTR_4g012850 24.342 304 201 8 77 373 5 286 8.56e-16 77.8
MS.gene46786.t1 MTR_3g100570 25.839 298 180 10 93 361 6 291 8.58e-16 77.8
MS.gene46786.t1 MTR_8g066975 26.316 285 185 9 92 363 7 279 1.96e-15 76.6
MS.gene46786.t1 MTR_6g091910 26.866 201 136 5 89 285 15 208 2.10e-15 75.1
MS.gene46786.t1 MTR_7g084400 31.122 196 109 5 93 282 207 382 3.57e-15 77.0
MS.gene46786.t1 MTR_7g066690 25.000 272 187 7 89 354 13 273 2.81e-14 73.2
MS.gene46786.t1 MTR_5g081150 28.966 145 97 2 83 223 24 166 1.01e-13 70.9
MS.gene46786.t1 MTR_5g081150 28.966 145 97 2 83 223 24 166 1.38e-13 70.5
MS.gene46786.t1 MTR_4g018750 25.461 271 181 9 99 361 21 278 1.89e-13 70.9
MS.gene46786.t1 MTR_2g436930 29.231 195 111 5 200 373 40 228 6.75e-13 68.2
MS.gene46786.t1 MTR_8g090210 23.649 296 180 10 96 368 113 385 1.74e-12 68.6
MS.gene46786.t1 MTR_4g018750 25.000 256 171 9 99 346 21 263 1.65e-11 64.7
MS.gene46786.t1 MTR_5g032420 21.405 299 201 6 93 361 6 300 4.84e-11 63.9
MS.gene46786.t1 MTR_1g088995 24.444 270 177 7 92 354 32 281 6.72e-11 63.2
MS.gene46786.t1 MTR_3g462140 24.296 284 191 8 89 362 11 280 9.67e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46786.t1 AT5G13490 80.916 393 63 5 1 389 1 385 0.0 608
MS.gene46786.t1 AT5G13490 80.916 393 63 5 1 389 1 385 0.0 608
MS.gene46786.t1 AT3G08580 82.188 393 54 7 1 389 1 381 0.0 589
MS.gene46786.t1 AT3G08580 82.188 393 54 7 1 389 1 381 0.0 589
MS.gene46786.t1 AT4G28390 74.680 391 82 4 2 388 1 378 0.0 548
MS.gene46786.t1 AT4G28390 74.680 391 82 4 2 388 1 378 0.0 548
MS.gene46786.t1 AT5G17400 59.136 301 123 0 80 380 1 301 3.89e-130 376
MS.gene46786.t1 AT5G56450 39.159 309 169 7 80 376 21 322 3.07e-66 213
MS.gene46786.t1 AT3G55640 28.037 321 213 7 58 368 1 313 3.37e-33 127
MS.gene46786.t1 AT3G55640 28.037 321 213 7 58 368 1 313 3.37e-33 127
MS.gene46786.t1 AT2G37890 30.640 297 189 7 92 379 44 332 7.92e-33 126
MS.gene46786.t1 AT1G14560 30.249 281 170 6 104 368 38 308 9.38e-33 125
MS.gene46786.t1 AT1G14560 30.545 275 165 6 104 362 38 302 4.61e-32 123
MS.gene46786.t1 AT4G26180 31.270 307 177 9 89 377 17 307 1.22e-31 122
MS.gene46786.t1 AT4G26180 31.959 291 165 8 89 362 17 291 1.25e-30 119
MS.gene46786.t1 AT3G53940 29.831 295 187 8 85 368 63 348 4.00e-29 116
MS.gene46786.t1 AT3G53940 23.858 197 138 3 91 287 178 362 1.86e-11 65.5
MS.gene46786.t1 AT3G51870 32.979 282 160 9 104 379 102 360 8.36e-29 115
MS.gene46786.t1 AT4G01100 27.597 308 168 9 96 368 45 332 2.58e-26 108
MS.gene46786.t1 AT5G01500 30.466 279 171 9 104 379 130 388 3.63e-26 109
MS.gene46786.t1 AT4G32400 29.893 281 171 9 93 368 114 373 1.27e-25 107
MS.gene46786.t1 AT4G01100 27.796 313 175 9 96 368 45 346 8.22e-25 104
MS.gene46786.t1 AT4G01100 26.821 302 166 9 102 368 1 282 2.80e-24 102
MS.gene46786.t1 AT5G51050 30.175 285 166 7 93 368 223 483 6.21e-24 103
MS.gene46786.t1 AT5G61810 27.703 296 183 8 77 368 52 320 3.26e-23 99.8
MS.gene46786.t1 AT3G21390 26.625 323 198 7 83 373 9 324 3.49e-23 99.8
MS.gene46786.t1 AT3G21390 26.625 323 198 7 83 373 31 346 4.18e-23 99.8
MS.gene46786.t1 AT5G61810 27.703 296 183 8 77 368 195 463 2.93e-22 98.6
MS.gene46786.t1 AT3G55640 27.481 262 171 6 58 312 1 250 3.87e-22 95.1
MS.gene46786.t1 AT3G55640 27.481 262 171 6 58 312 1 250 3.87e-22 95.1
MS.gene46786.t1 AT1G14560 31.383 188 112 4 197 368 24 210 7.72e-22 93.6
MS.gene46786.t1 AT3G20240 24.917 301 190 6 86 368 45 327 8.83e-22 95.9
MS.gene46786.t1 AT5G48970 27.160 324 196 10 83 373 12 328 2.02e-21 94.7
MS.gene46786.t1 AT5G07320 27.778 270 169 7 102 368 218 464 9.11e-21 94.4
MS.gene46786.t1 AT3G53940 29.167 216 137 5 85 293 63 269 9.84e-19 86.3
MS.gene46786.t1 AT5G64970 26.780 295 172 11 96 368 138 410 5.29e-15 76.6
MS.gene46786.t1 AT5G48970 26.641 259 157 8 141 373 6 257 7.10e-15 74.7
MS.gene46786.t1 AT1G14140 23.881 268 189 6 93 354 16 274 8.79e-15 74.7
MS.gene46786.t1 AT1G14140 28.061 196 127 5 198 383 18 209 1.08e-11 65.9
MS.gene46786.t1 AT5G64970 26.441 295 173 11 96 368 14 286 2.12e-14 73.6
MS.gene46786.t1 AT5G64970 27.431 288 165 11 96 361 138 403 4.82e-14 73.6
MS.gene46786.t1 AT5G46800 26.573 286 186 8 92 363 7 282 5.35e-14 72.4
MS.gene46786.t1 AT5G46800 26.573 286 186 8 92 363 7 282 5.35e-14 72.4
MS.gene46786.t1 AT5G46800 26.573 286 186 8 92 363 7 282 5.35e-14 72.4
MS.gene46786.t1 AT5G58970 24.399 291 191 8 83 361 6 279 9.93e-14 71.6
MS.gene46786.t1 AT2G33820 23.759 282 200 5 89 362 14 288 2.52e-13 70.5
MS.gene46786.t1 AT5G66380 27.317 205 125 6 191 383 5 197 1.25e-12 68.6
MS.gene46786.t1 AT3G54110 23.134 268 189 8 93 354 15 271 2.05e-12 67.8
MS.gene46786.t1 AT1G79900 25.726 241 156 6 140 373 53 277 7.22e-12 66.2
MS.gene46786.t1 AT1G78180 24.415 299 174 8 96 368 131 403 1.23e-11 66.2
MS.gene46786.t1 AT2G33820 23.103 290 200 6 89 362 14 296 5.02e-11 63.9

Find 120 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGTGCTGGTGTGCTTTC+TGG 0.149238 3.3:+21025195 MS.gene46786:CDS
TGATCATCTCATCCTGGTTT+TGG 0.179358 3.3:-21024230 None:intergenic
CTGGTCTTTCATCTGGATTT+AGG 0.213706 3.3:+21023944 MS.gene46786:CDS
TGGTCTTTCATCTGGATTTA+GGG 0.236214 3.3:+21023945 MS.gene46786:CDS
AATGGGAGCAGCAGCAGTTT+TGG 0.270825 3.3:-21024187 None:intergenic
AACATCCTCCGTGCCGTTGC+TGG 0.301686 3.3:+21025177 MS.gene46786:CDS
AGTTCCCATAGGAGAATCTT+TGG 0.306546 3.3:-21024002 None:intergenic
CTTAGCTGCCTTAGAATCAT+TGG 0.323339 3.3:-21024635 None:intergenic
CTTGCTACTGATGGAATTGC+TGG 0.330650 3.3:+21024709 MS.gene46786:CDS
AGAAGGACAGGGATGGTTAC+TGG 0.330831 3.3:+21024518 MS.gene46786:CDS
TCTTCTCACTGGAAAATTGC+AGG 0.333134 3.3:+21024819 MS.gene46786:CDS
CCTCATTCTTGAGAATTTGT+TGG 0.333777 3.3:-21025122 None:intergenic
TGTCGATCTGGTCTTTCATC+TGG 0.342815 3.3:+21023937 MS.gene46786:CDS
CTCCGTGCCGTTGCTGGTGC+TGG 0.343034 3.3:+21025183 MS.gene46786:CDS
CTTCTCCTAACAGTGTCAAT+TGG 0.350865 3.3:-21025045 None:intergenic
ACAATCACCAATACCCTTGT+AGG 0.369255 3.3:-21024274 None:intergenic
ATGAGGGTGTTGTTGCTCTC+TGG 0.371630 3.3:+21024316 MS.gene46786:CDS
ACCTCCAGATGCCAAGTTAC+CGG 0.389000 3.3:-21024551 None:intergenic
GTGACCTTCTCCATGATTGT+TGG 0.389407 3.3:-21023901 None:intergenic
CAAAAGCTTAATACGTTCAA+TGG 0.391915 3.3:-21024205 None:intergenic
TCGTCTTGCCAATGATTCTA+AGG 0.397580 3.3:+21024627 MS.gene46786:CDS
ACAGGGATGGTTACTGGAAA+TGG 0.399761 3.3:+21024524 MS.gene46786:CDS
GGCCAGCTCCACTGTCGATC+TGG 0.406307 3.3:+21023925 MS.gene46786:CDS
TTCTGGTTACGACAAGTTGC+AGG 0.414142 3.3:+21025212 MS.gene46786:CDS
GAAAGCAACCATGGTTGATC+AGG 0.420030 3.3:+21023870 None:intergenic
TTGGCATCTGGAGGTGCTGC+TGG 0.420414 3.3:+21024559 MS.gene46786:CDS
TTCTCCTAACAGTGTCAATT+GGG 0.434457 3.3:-21025044 None:intergenic
CTCAAACCTGTTCTTCTCAC+TGG 0.437345 3.3:+21024808 MS.gene46786:CDS
TTTCGTTATTGACTTTCTCA+TGG 0.438686 3.3:+21024143 MS.gene46786:CDS
CTTGTGTTCGGCAAGAAATA+CGG 0.440747 3.3:+21025237 MS.gene46786:CDS
AAGTTGCAGGTTCTTGTGTT+CGG 0.444616 3.3:+21025225 MS.gene46786:CDS
TTTGCCGGTAACTTGGCATC+TGG 0.445959 3.3:+21024547 MS.gene46786:CDS
CATACCCAATTGACACTGTT+AGG 0.447208 3.3:+21025040 MS.gene46786:CDS
GAGCCATCCAAGCGCAAAGC+TGG 0.450450 3.3:-21024992 None:intergenic
GGTTACTGGAAATGGTTTGC+CGG 0.452634 3.3:+21024532 MS.gene46786:CDS
GAAATGGTTTGCCGGTAACT+TGG 0.453917 3.3:+21024540 MS.gene46786:CDS
AGAAGAATGATGATGACATC+TGG 0.454405 3.3:+21025063 MS.gene46786:CDS
CAGTGAGAAGAACAGGTTTG+AGG 0.454558 3.3:-21024807 None:intergenic
AGTGAGAAGAACAGGTTTGA+GGG 0.454604 3.3:-21024806 None:intergenic
TGAGAAAGTCAATAACGAAA+TGG 0.456385 3.3:-21024140 None:intergenic
TCGCCAGCTTTGCGCTTGGA+TGG 0.458299 3.3:+21024989 MS.gene46786:CDS
GCTGGAGCAGCGACGAAGAC+GGG 0.467128 3.3:-21024108 None:intergenic
CACCAGCACCAGCAACGGCA+CGG 0.468876 3.3:-21025185 None:intergenic
GTTCCCATAGGAGAATCTTT+GGG 0.471265 3.3:-21024001 None:intergenic
TCTGGATTTAGGGGTTATGA+AGG 0.477435 3.3:+21023955 MS.gene46786:CDS
ATCATTGGCAAGACGAGTAC+GGG 0.477887 3.3:-21024620 None:intergenic
CCAACAAATTCTCAAGAATG+AGG 0.482221 3.3:+21025122 MS.gene46786:CDS
ATCAACAGTTGCCCTGCAAG+TGG 0.483410 3.3:-21024058 None:intergenic
TCTGAGCCCTACAAGGGTAT+TGG 0.488103 3.3:+21024267 MS.gene46786:CDS
AAAAGCTTAATACGTTCAAT+GGG 0.489695 3.3:-21024204 None:intergenic
GACCAGATCGACAGTGGAGC+TGG 0.489976 3.3:-21023927 None:intergenic
ATGTCCCAAAGATTCTCCTA+TGG 0.492338 3.3:+21023997 MS.gene46786:CDS
GGTTTCAACATCTCCTGTGT+TGG 0.492793 3.3:+21024742 MS.gene46786:CDS
TTCGTTATTGACTTTCTCAT+GGG 0.493555 3.3:+21024144 MS.gene46786:CDS
CAGGATGAGATGATCAAGTC+TGG 0.496357 3.3:+21024237 MS.gene46786:CDS
AAGCACACCAGCACCAGCAA+CGG 0.501311 3.3:-21025190 None:intergenic
GGGTGCTAAGTCATTGTTCA+AGG 0.502290 3.3:+21025143 MS.gene46786:CDS
AATCATTGGCAAGACGAGTA+CGG 0.503375 3.3:-21024621 None:intergenic
GTCTACCGTGGTCTGTACTT+CGG 0.505449 3.3:+21024772 MS.gene46786:CDS
AAGAAGACTCTTGCTACTGA+TGG 0.508472 3.3:+21024700 MS.gene46786:CDS
TAAAAGAACAATGGCTGATG+AGG 0.509418 3.3:+21024299 MS.gene46786:CDS
TAACCTGAGTGGGGAAATAA+CGG 0.511945 3.3:-21024362 None:intergenic
ACAATCATGGAGAAGGTCAC+TGG 0.514525 3.3:+21023904 MS.gene46786:CDS
AGAAAACAACTAACCTGAGT+GGG 0.518937 3.3:-21024372 None:intergenic
TTCGGCAAGAAATACGGATC+TGG 0.519522 3.3:+21025243 MS.gene46786:CDS
CAGACTTGATCATCTCATCC+TGG 0.520517 3.3:-21024236 None:intergenic
AAGTCATTGTTCAAGGGAGC+TGG 0.521554 3.3:+21025150 MS.gene46786:CDS
GGTTAAGCAACCAACAATCA+TGG 0.524656 3.3:+21023891 MS.gene46786:CDS
TGATGCTAGACCTGCACCAT+TGG 0.527310 3.3:-21025019 None:intergenic
CAACAGTTGCCCTGCAAGTG+GGG 0.528651 3.3:-21024056 None:intergenic
TGGCTCATTACCAATGGTGC+AGG 0.529441 3.3:+21025009 MS.gene46786:CDS
TAGGCTCTCTGAGCCCTACA+AGG 0.529504 3.3:+21024260 MS.gene46786:CDS
GGTCTTTCATCTGGATTTAG+GGG 0.530879 3.3:+21023946 MS.gene46786:CDS
CATTCAAAGATTACTTCAAG+AGG 0.536246 3.3:+21024482 MS.gene46786:CDS
GATTCTAAGGCAGCTAAGAA+AGG 0.539062 3.3:+21024640 MS.gene46786:CDS
CAGCTAAGAAAGGTGGAGAA+AGG 0.539801 3.3:+21024650 MS.gene46786:CDS
GCCGGTAACTTGGCATCTGG+AGG 0.547072 3.3:+21024550 MS.gene46786:CDS
GAGAATCTTTGGGACATGGC+AGG 0.547914 3.3:-21023991 None:intergenic
GTTATTGACTTTCTCATGGG+TGG 0.552168 3.3:+21024147 MS.gene46786:CDS
TTGCTACTGATGGAATTGCT+GGG 0.552525 3.3:+21024710 MS.gene46786:CDS
TCAACAGTTGCCCTGCAAGT+GGG 0.556580 3.3:-21024057 None:intergenic
AATTTGTTGGAATGCATCAA+AGG 0.558728 3.3:-21025109 None:intergenic
AAAAGAACAATGGCTGATGA+GGG 0.560769 3.3:+21024300 MS.gene46786:CDS
GGACATGGCAGGATGGCGGT+AGG 0.563819 3.3:-21023980 None:intergenic
TGTCCCAAAGATTCTCCTAT+GGG 0.564873 3.3:+21023998 MS.gene46786:CDS
TATCCGTTATTTCCCCACTC+AGG 0.571053 3.3:+21024359 MS.gene46786:CDS
CAACAAATTCTCAAGAATGA+GGG 0.571067 3.3:+21025123 MS.gene46786:CDS
GGTGGAGAAAGGCAGTTCAA+TGG 0.572074 3.3:+21024661 MS.gene46786:CDS
TAGAAAACAACTAACCTGAG+TGG 0.579173 3.3:-21024373 None:intergenic
CTTGGATGGCTCATTACCAA+TGG 0.580145 3.3:+21025003 MS.gene46786:CDS
CAATCACCAATACCCTTGTA+GGG 0.581476 3.3:-21024273 None:intergenic
TGCTGGAGCAGCGACGAAGA+CGG 0.584125 3.3:-21024109 None:intergenic
AACAGTTGCCCTGCAAGTGG+GGG 0.584275 3.3:-21024055 None:intergenic
ATGAGATGATCAAGTCTGGT+AGG 0.599652 3.3:+21024241 MS.gene46786:CDS
CAAGAGAGTAAACAAACAAG+AGG 0.602030 3.3:-21024591 None:intergenic
ATCTTTGGGACATGGCAGGA+TGG 0.604045 3.3:-21023987 None:intergenic
GGTGTTGTTGCTCTCTGGAG+AGG 0.606867 3.3:+21024321 MS.gene46786:CDS
AACTTCAAGAAGGACAGGGA+TGG 0.606997 3.3:+21024511 MS.gene46786:CDS
CGCTGCATTGCAGAATCCAA+TGG 0.607456 3.3:+21024032 MS.gene46786:CDS
GCAACCAACAATCATGGAGA+AGG 0.628211 3.3:+21023897 MS.gene46786:CDS
GGCAAGAAATACGGATCTGG+TGG 0.628657 3.3:+21025246 MS.gene46786:CDS
TCTAAGGCAGCTAAGAAAGG+TGG 0.629619 3.3:+21024643 MS.gene46786:CDS
GTAAACAAACAAGAGGGATG+AGG 0.631365 3.3:-21024584 None:intergenic
CACAGGAGATGTTGAAACCA+CGG 0.633206 3.3:-21024738 None:intergenic
ATGAAAGACCAGATCGACAG+TGG 0.633471 3.3:-21023933 None:intergenic
ATAGGAGAATCTTTGGGACA+TGG 0.635000 3.3:-21023995 None:intergenic
TTTCAACTTCAAGAAGGACA+GGG 0.637221 3.3:+21024507 MS.gene46786:CDS
GGTGCTAAGTCATTGTTCAA+GGG 0.642143 3.3:+21025144 MS.gene46786:CDS
GGAATTGCTGGGCTTTACCG+TGG 0.654046 3.3:+21024721 MS.gene46786:CDS
AAGAGAGTAAACAAACAAGA+GGG 0.655445 3.3:-21024590 None:intergenic
GTAGACAATGATTCCAACAC+AGG 0.657927 3.3:-21024755 None:intergenic
GGGGAAATAACGGATAACAT+TGG 0.658006 3.3:-21024352 None:intergenic
AGCGTTTGAGTAGTTCCCAT+AGG 0.663946 3.3:-21024013 None:intergenic
CAGCACCAGCAACGGCACGG+AGG 0.668727 3.3:-21025182 None:intergenic
GAAAACAACTAACCTGAGTG+GGG 0.671762 3.3:-21024371 None:intergenic
TTGCTTAACCTGATCAACCA+TGG 0.675233 3.3:-21023878 None:intergenic
AGGCTCTCTGAGCCCTACAA+GGG 0.676799 3.3:+21024261 MS.gene46786:CDS
TTTGGGACATGGCAGGATGG+CGG 0.679753 3.3:-21023984 None:intergenic
GTTGGAATCATTGTCTACCG+TGG 0.700486 3.3:+21024760 MS.gene46786:CDS
ACAATCCGAAGTACAGACCA+CGG 0.718426 3.3:-21024777 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGCTATTTAAATTGTTTT+AGG + chr3.3:21024447-21024466 MS.gene46786:intron 15.0%
!! AATGAATATCATTTGTTCAA+AGG - chr3.3:21024858-21024877 None:intergenic 20.0%
!! ATGAATATCATTTGTTCAAA+GGG - chr3.3:21024857-21024876 None:intergenic 20.0%
!! TGCAACGAAAAATAATAATT+TGG - chr3.3:21024885-21024904 None:intergenic 20.0%
!!! TGATTGTTTTAAAAGAACAA+TGG + chr3.3:21024290-21024309 MS.gene46786:CDS 20.0%
! AAAAGCTTAATACGTTCAAT+GGG - chr3.3:21024207-21024226 None:intergenic 25.0%
! CACATTAAATACACAACAAT+TGG - chr3.3:21024419-21024438 None:intergenic 25.0%
AAGAGAGTAAACAAACAAGA+GGG - chr3.3:21024593-21024612 None:intergenic 30.0%
CAAAAGCTTAATACGTTCAA+TGG - chr3.3:21024208-21024227 None:intergenic 30.0%
CAACAAATTCTCAAGAATGA+GGG + chr3.3:21025123-21025142 MS.gene46786:CDS 30.0%
CATTCAAAGATTACTTCAAG+AGG + chr3.3:21024482-21024501 MS.gene46786:CDS 30.0%
TAAATACACAACAATTGGAC+AGG - chr3.3:21024414-21024433 None:intergenic 30.0%
TGAGAAAGTCAATAACGAAA+TGG - chr3.3:21024143-21024162 None:intergenic 30.0%
! AAATCTAAAGTATCTACCAG+TGG - chr3.3:21024914-21024933 None:intergenic 30.0%
! TTTTGAACTCTTGACATTAC+AGG + chr3.3:21024957-21024976 MS.gene46786:intron 30.0%
!! AATTTGTTGGAATGCATCAA+AGG - chr3.3:21025112-21025131 None:intergenic 30.0%
!! TTCGTTATTGACTTTCTCAT+GGG + chr3.3:21024144-21024163 MS.gene46786:CDS 30.0%
!! TTTCGTTATTGACTTTCTCA+TGG + chr3.3:21024143-21024162 MS.gene46786:CDS 30.0%
AAAAGAACAATGGCTGATGA+GGG + chr3.3:21024300-21024319 MS.gene46786:CDS 35.0%
AGAAAACAACTAACCTGAGT+GGG - chr3.3:21024375-21024394 None:intergenic 35.0%
AGTTCAAAAGATTTCAGCAG+AGG - chr3.3:21024946-21024965 None:intergenic 35.0%
CAAGAGAGTAAACAAACAAG+AGG - chr3.3:21024594-21024613 None:intergenic 35.0%
CCAACAAATTCTCAAGAATG+AGG + chr3.3:21025122-21025141 MS.gene46786:CDS 35.0%
CCTCATTCTTGAGAATTTGT+TGG - chr3.3:21025125-21025144 None:intergenic 35.0%
TAAAAGAACAATGGCTGATG+AGG + chr3.3:21024299-21024318 MS.gene46786:CDS 35.0%
TAGAAAACAACTAACCTGAG+TGG - chr3.3:21024376-21024395 None:intergenic 35.0%
TGGTCTTTCATCTGGATTTA+GGG + chr3.3:21023945-21023964 MS.gene46786:CDS 35.0%
TTCTCCTAACAGTGTCAATT+GGG - chr3.3:21025047-21025066 None:intergenic 35.0%
TTTCAACTTCAAGAAGGACA+GGG + chr3.3:21024507-21024526 MS.gene46786:CDS 35.0%
! AATTTTCCAGTGAGAAGAAC+AGG - chr3.3:21024817-21024836 None:intergenic 35.0%
! AGAAGAATGATGATGACATC+TGG + chr3.3:21025063-21025082 MS.gene46786:CDS 35.0%
! TAAGCTTTTGATCCAAAACC+AGG + chr3.3:21024218-21024237 MS.gene46786:CDS 35.0%
! TTTTCAACTTCAAGAAGGAC+AGG + chr3.3:21024506-21024525 MS.gene46786:CDS 35.0%
AAGAAGACTCTTGCTACTGA+TGG + chr3.3:21024700-21024719 MS.gene46786:CDS 40.0%
AAGTTGCAGGTTCTTGTGTT+CGG + chr3.3:21025225-21025244 MS.gene46786:CDS 40.0%
AATCATTGGCAAGACGAGTA+CGG - chr3.3:21024624-21024643 None:intergenic 40.0%
ACAATCACCAATACCCTTGT+AGG - chr3.3:21024277-21024296 None:intergenic 40.0%
AGTGAGAAGAACAGGTTTGA+GGG - chr3.3:21024809-21024828 None:intergenic 40.0%
AGTTCCCATAGGAGAATCTT+TGG - chr3.3:21024005-21024024 None:intergenic 40.0%
ATGAGATGATCAAGTCTGGT+AGG + chr3.3:21024241-21024260 MS.gene46786:CDS 40.0%
ATGTCCCAAAGATTCTCCTA+TGG + chr3.3:21023997-21024016 MS.gene46786:CDS 40.0%
CAATCACCAATACCCTTGTA+GGG - chr3.3:21024276-21024295 None:intergenic 40.0%
CATACCCAATTGACACTGTT+AGG + chr3.3:21025040-21025059 MS.gene46786:CDS 40.0%
CTTAGCTGCCTTAGAATCAT+TGG - chr3.3:21024638-21024657 None:intergenic 40.0%
CTTCTCCTAACAGTGTCAAT+TGG - chr3.3:21025048-21025067 None:intergenic 40.0%
CTTGTGTTCGGCAAGAAATA+CGG + chr3.3:21025237-21025256 MS.gene46786:CDS 40.0%
GAAAACAACTAACCTGAGTG+GGG - chr3.3:21024374-21024393 None:intergenic 40.0%
GGTCTTTCATCTGGATTTAG+GGG + chr3.3:21023946-21023965 MS.gene46786:CDS 40.0%
GGTGCTAAGTCATTGTTCAA+GGG + chr3.3:21025144-21025163 MS.gene46786:CDS 40.0%
GGTTAAGCAACCAACAATCA+TGG + chr3.3:21023891-21023910 MS.gene46786:CDS 40.0%
GTAAACAAACAAGAGGGATG+AGG - chr3.3:21024587-21024606 None:intergenic 40.0%
GTAGACAATGATTCCAACAC+AGG - chr3.3:21024758-21024777 None:intergenic 40.0%
GTTCCCATAGGAGAATCTTT+GGG - chr3.3:21024004-21024023 None:intergenic 40.0%
TAACCTGAGTGGGGAAATAA+CGG - chr3.3:21024365-21024384 None:intergenic 40.0%
TCGTCTTGCCAATGATTCTA+AGG + chr3.3:21024627-21024646 MS.gene46786:CDS 40.0%
TCTGGATTTAGGGGTTATGA+AGG + chr3.3:21023955-21023974 MS.gene46786:CDS 40.0%
TCTTCTCACTGGAAAATTGC+AGG + chr3.3:21024819-21024838 MS.gene46786:CDS 40.0%
TGTCCCAAAGATTCTCCTAT+GGG + chr3.3:21023998-21024017 MS.gene46786:CDS 40.0%
TTGCTACTGATGGAATTGCT+GGG + chr3.3:21024710-21024729 MS.gene46786:CDS 40.0%
! CTGGTCTTTCATCTGGATTT+AGG + chr3.3:21023944-21023963 MS.gene46786:CDS 40.0%
! GAGGCTTTTCAACTTCAAGA+AGG + chr3.3:21024501-21024520 MS.gene46786:CDS 40.0%
! TGATCATCTCATCCTGGTTT+TGG - chr3.3:21024233-21024252 None:intergenic 40.0%
!! ATAGGAGAATCTTTGGGACA+TGG - chr3.3:21023998-21024017 None:intergenic 40.0%
!! GATTCTAAGGCAGCTAAGAA+AGG + chr3.3:21024640-21024659 MS.gene46786:CDS 40.0%
!! GGGGAAATAACGGATAACAT+TGG - chr3.3:21024355-21024374 None:intergenic 40.0%
!! GTTATTGACTTTCTCATGGG+TGG + chr3.3:21024147-21024166 MS.gene46786:CDS 40.0%
AACTTCAAGAAGGACAGGGA+TGG + chr3.3:21024511-21024530 MS.gene46786:CDS 45.0%
AAGTCATTGTTCAAGGGAGC+TGG + chr3.3:21025150-21025169 MS.gene46786:CDS 45.0%
ACAATCATGGAGAAGGTCAC+TGG + chr3.3:21023904-21023923 MS.gene46786:CDS 45.0%
ACAATCCGAAGTACAGACCA+CGG - chr3.3:21024780-21024799 None:intergenic 45.0%
ACAGGGATGGTTACTGGAAA+TGG + chr3.3:21024524-21024543 MS.gene46786:CDS 45.0%
AGCGTTTGAGTAGTTCCCAT+AGG - chr3.3:21024016-21024035 None:intergenic 45.0%
ATCATTGGCAAGACGAGTAC+GGG - chr3.3:21024623-21024642 None:intergenic 45.0%
ATGAAAGACCAGATCGACAG+TGG - chr3.3:21023936-21023955 None:intergenic 45.0%
CACAGGAGATGTTGAAACCA+CGG - chr3.3:21024741-21024760 None:intergenic 45.0%
CAGACTTGATCATCTCATCC+TGG - chr3.3:21024239-21024258 None:intergenic 45.0%
CAGCTAAGAAAGGTGGAGAA+AGG + chr3.3:21024650-21024669 MS.gene46786:CDS 45.0%
CAGGATGAGATGATCAAGTC+TGG + chr3.3:21024237-21024256 MS.gene46786:CDS 45.0%
CAGTGAGAAGAACAGGTTTG+AGG - chr3.3:21024810-21024829 None:intergenic 45.0%
CTCAAACCTGTTCTTCTCAC+TGG + chr3.3:21024808-21024827 MS.gene46786:CDS 45.0%
CTTGCTACTGATGGAATTGC+TGG + chr3.3:21024709-21024728 MS.gene46786:CDS 45.0%
CTTGGATGGCTCATTACCAA+TGG + chr3.3:21025003-21025022 MS.gene46786:CDS 45.0%
GCAACCAACAATCATGGAGA+AGG + chr3.3:21023897-21023916 MS.gene46786:CDS 45.0%
GGGTGCTAAGTCATTGTTCA+AGG + chr3.3:21025143-21025162 MS.gene46786:CDS 45.0%
GGTTTCAACATCTCCTGTGT+TGG + chr3.3:21024742-21024761 MS.gene46786:CDS 45.0%
GTGACCTTCTCCATGATTGT+TGG - chr3.3:21023904-21023923 None:intergenic 45.0%
GTTGGAATCATTGTCTACCG+TGG + chr3.3:21024760-21024779 MS.gene46786:CDS 45.0%
TTCGGCAAGAAATACGGATC+TGG + chr3.3:21025243-21025262 MS.gene46786:CDS 45.0%
! ACGAAATGGCTCTTTTCTGC+TGG - chr3.3:21024129-21024148 None:intergenic 45.0%
! GAAATGGTTTGCCGGTAACT+TGG + chr3.3:21024540-21024559 MS.gene46786:CDS 45.0%
! GGTTACTGGAAATGGTTTGC+CGG + chr3.3:21024532-21024551 MS.gene46786:CDS 45.0%
! TATCCGTTATTTCCCCACTC+AGG + chr3.3:21024359-21024378 MS.gene46786:CDS 45.0%
! TCTAAGGCAGCTAAGAAAGG+TGG + chr3.3:21024643-21024662 MS.gene46786:CDS 45.0%
! TGTCGATCTGGTCTTTCATC+TGG + chr3.3:21023937-21023956 MS.gene46786:CDS 45.0%
! TTCTGGTTACGACAAGTTGC+AGG + chr3.3:21025212-21025231 MS.gene46786:CDS 45.0%
!!! AGCAGCAGCAGTTTTGGAAA+CGG - chr3.3:21024184-21024203 None:intergenic 45.0%
ACCTCCAGATGCCAAGTTAC+CGG - chr3.3:21024554-21024573 None:intergenic 50.0%
AGAAGGACAGGGATGGTTAC+TGG + chr3.3:21024518-21024537 MS.gene46786:CDS 50.0%
ATCAACAGTTGCCCTGCAAG+TGG - chr3.3:21024061-21024080 None:intergenic 50.0%
CGCTGCATTGCAGAATCCAA+TGG + chr3.3:21024032-21024051 MS.gene46786:CDS 50.0%
GGCAAGAAATACGGATCTGG+TGG + chr3.3:21025246-21025265 MS.gene46786:CDS 50.0%
GGTGGAGAAAGGCAGTTCAA+TGG + chr3.3:21024661-21024680 MS.gene46786:CDS 50.0%
GTCTACCGTGGTCTGTACTT+CGG + chr3.3:21024772-21024791 MS.gene46786:CDS 50.0%
TCAACAGTTGCCCTGCAAGT+GGG - chr3.3:21024060-21024079 None:intergenic 50.0%
TGATGCTAGACCTGCACCAT+TGG - chr3.3:21025022-21025041 None:intergenic 50.0%
TGGCTCATTACCAATGGTGC+AGG + chr3.3:21025009-21025028 MS.gene46786:CDS 50.0%
TTTCGTTGCAAGCATGCCAC+TGG + chr3.3:21024895-21024914 MS.gene46786:intron 50.0%
TTTGCCGGTAACTTGGCATC+TGG + chr3.3:21024547-21024566 MS.gene46786:CDS 50.0%
! AATGGGAGCAGCAGCAGTTT+TGG - chr3.3:21024190-21024209 None:intergenic 50.0%
! TCTGAGCCCTACAAGGGTAT+TGG + chr3.3:21024267-21024286 MS.gene46786:CDS 50.0%
! TTTTTCGCCAGCTTTGCGCT+TGG + chr3.3:21024985-21025004 MS.gene46786:CDS 50.0%
!! ATCTTTGGGACATGGCAGGA+TGG - chr3.3:21023990-21024009 None:intergenic 50.0%
!! ATGAGGGTGTTGTTGCTCTC+TGG + chr3.3:21024316-21024335 MS.gene46786:CDS 50.0%
!! GAGAATCTTTGGGACATGGC+AGG - chr3.3:21023994-21024013 None:intergenic 50.0%
AACAGTTGCCCTGCAAGTGG+GGG - chr3.3:21024058-21024077 None:intergenic 55.0%
AAGCACACCAGCACCAGCAA+CGG - chr3.3:21025193-21025212 None:intergenic 55.0%
AGGCTCTCTGAGCCCTACAA+GGG + chr3.3:21024261-21024280 MS.gene46786:CDS 55.0%
CAACAGTTGCCCTGCAAGTG+GGG - chr3.3:21024059-21024078 None:intergenic 55.0%
GGAATTGCTGGGCTTTACCG+TGG + chr3.3:21024721-21024740 MS.gene46786:CDS 55.0%
TAGGCTCTCTGAGCCCTACA+AGG + chr3.3:21024260-21024279 MS.gene46786:CDS 55.0%
! TTTGGGACATGGCAGGATGG+CGG - chr3.3:21023987-21024006 None:intergenic 55.0%
!! GGTGTTGTTGCTCTCTGGAG+AGG + chr3.3:21024321-21024340 MS.gene46786:CDS 55.0%
ATCCAATGGCCCCCACTTGC+AGG + chr3.3:21024046-21024065 MS.gene46786:CDS 60.0%
GACCAGATCGACAGTGGAGC+TGG - chr3.3:21023930-21023949 None:intergenic 60.0%
GAGCCATCCAAGCGCAAAGC+TGG - chr3.3:21024995-21025014 None:intergenic 60.0%
GCCGGTAACTTGGCATCTGG+AGG + chr3.3:21024550-21024569 MS.gene46786:CDS 60.0%
TCCAATGGCCCCCACTTGCA+GGG + chr3.3:21024047-21024066 MS.gene46786:CDS 60.0%
TCGCCAGCTTTGCGCTTGGA+TGG + chr3.3:21024989-21025008 MS.gene46786:CDS 60.0%
TGCTGGAGCAGCGACGAAGA+CGG - chr3.3:21024112-21024131 None:intergenic 60.0%
! AACATCCTCCGTGCCGTTGC+TGG + chr3.3:21025177-21025196 MS.gene46786:CDS 60.0%
!! GCTGGTGCTGGTGTGCTTTC+TGG + chr3.3:21025195-21025214 MS.gene46786:CDS 60.0%
!! TTGGCATCTGGAGGTGCTGC+TGG + chr3.3:21024559-21024578 MS.gene46786:CDS 60.0%
CACCAGCACCAGCAACGGCA+CGG - chr3.3:21025188-21025207 None:intergenic 65.0%
GCTGGAGCAGCGACGAAGAC+GGG - chr3.3:21024111-21024130 None:intergenic 65.0%
GGCCAGCTCCACTGTCGATC+TGG + chr3.3:21023925-21023944 MS.gene46786:CDS 65.0%
! GGACATGGCAGGATGGCGGT+AGG - chr3.3:21023983-21024002 None:intergenic 65.0%
CAGCACCAGCAACGGCACGG+AGG - chr3.3:21025185-21025204 None:intergenic 70.0%
GCCCTGCAAGTGGGGGCCAT+TGG - chr3.3:21024051-21024070 None:intergenic 70.0%
! CTCCGTGCCGTTGCTGGTGC+TGG + chr3.3:21025183-21025202 MS.gene46786:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 21023880 21025275 21023880 ID=MS.gene46786
chr3.3 mRNA 21023880 21025275 21023880 ID=MS.gene46786.t1;Parent=MS.gene46786
chr3.3 exon 21023880 21024380 21023880 ID=MS.gene46786.t1.exon1;Parent=MS.gene46786.t1
chr3.3 CDS 21023880 21024380 21023880 ID=cds.MS.gene46786.t1;Parent=MS.gene46786.t1
chr3.3 exon 21024469 21024840 21024469 ID=MS.gene46786.t1.exon2;Parent=MS.gene46786.t1
chr3.3 CDS 21024469 21024840 21024469 ID=cds.MS.gene46786.t1;Parent=MS.gene46786.t1
chr3.3 exon 21024979 21025275 21024979 ID=MS.gene46786.t1.exon3;Parent=MS.gene46786.t1
chr3.3 CDS 21024979 21025275 21024979 ID=cds.MS.gene46786.t1;Parent=MS.gene46786.t1
Gene Sequence

>MS.gene46786

ATGGTTGATCAGGTTAAGCAACCAACAATCATGGAGAAGGTCACTGGCCAGCTCCACTGTCGATCTGGTCTTTCATCTGGATTTAGGGGTTATGAAGGAACCTACCGCCATCCTGCCATGTCCCAAAGATTCTCCTATGGGAACTACTCAAACGCTGCATTGCAGAATCCAATGGCCCCCACTTGCAGGGCAACTGTTGATTTGTCAGCTGTTGCAACAACTGCTTCTCCCGTCTTCGTCGCTGCTCCAGCAGAAAAGAGCCATTTCGTTATTGACTTTCTCATGGGTGGTGTTTCAGCTGCCGTTTCCAAAACTGCTGCTGCTCCCATTGAACGTATTAAGCTTTTGATCCAAAACCAGGATGAGATGATCAAGTCTGGTAGGCTCTCTGAGCCCTACAAGGGTATTGGTGATTGTTTTAAAAGAACAATGGCTGATGAGGGTGTTGTTGCTCTCTGGAGAGGAAACACTGCCAATGTTATCCGTTATTTCCCCACTCAGGTTAGTTGTTTTCTAGTTTTGAATTTGACTCCTGTCCAATTGTTGTGTATTTAATGTGTTTCTGAATTTGCTATTTAAATTGTTTTAGGCTTTGAACTTTGCATTCAAAGATTACTTCAAGAGGCTTTTCAACTTCAAGAAGGACAGGGATGGTTACTGGAAATGGTTTGCCGGTAACTTGGCATCTGGAGGTGCTGCTGGTGCCTCATCCCTCTTGTTTGTTTACTCTCTTGATTATGCCCGTACTCGTCTTGCCAATGATTCTAAGGCAGCTAAGAAAGGTGGAGAAAGGCAGTTCAATGGTCTTGTTGATGTTTACAAGAAGACTCTTGCTACTGATGGAATTGCTGGGCTTTACCGTGGTTTCAACATCTCCTGTGTTGGAATCATTGTCTACCGTGGTCTGTACTTCGGATTGTATGATTCCCTCAAACCTGTTCTTCTCACTGGAAAATTGCAGGTTTGTTTTTTTACCCTTTGAACAAATGATATTCATTTTAACCAAATTATTATTTTTCGTTGCAAGCATGCCACTGGTAGATACTTTAGATTTTTATTCGATCCTCTGCTGAAATCTTTTGAACTCTTGACATTACAGGATAGCTTTTTCGCCAGCTTTGCGCTTGGATGGCTCATTACCAATGGTGCAGGTCTAGCATCATACCCAATTGACACTGTTAGGAGAAGAATGATGATGACATCTGGAGAAGCTGTCAAGTACAAGAGTTCCTTTGATGCATTCCAACAAATTCTCAAGAATGAGGGTGCTAAGTCATTGTTCAAGGGAGCTGGTGCTAACATCCTCCGTGCCGTTGCTGGTGCTGGTGTGCTTTCTGGTTACGACAAGTTGCAGGTTCTTGTGTTCGGCAAGAAATACGGATCTGGTGGTGCTTAA
Protein sequence

>MS.gene46786.t1

MVDQVKQPTIMEKVTGQLHCRSGLSSGFRGYEGTYRHPAMSQRFSYGNYSNAALQNPMAPTCRATVDLSAVATTASPVFVAAPAEKSHFVIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSEPYKGIGDCFKRTMADEGVVALWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDRDGYWKWFAGNLASGGAAGASSLLFVYSLDYARTRLANDSKAAKKGGERQFNGLVDVYKKTLATDGIAGLYRGFNISCVGIIVYRGLYFGLYDSLKPVLLTGKLQDSFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSFDAFQQILKNEGAKSLFKGAGANILRAVAGAGVLSGYDKLQVLVFGKKYGSGGA