AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene065947

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065947.t1 MTR_4g078545 97.574 371 8 1 1 370 1 371 0.0 735
MS.gene065947.t1 MTR_3g028590 74.425 391 75 4 1 369 5 392 0.0 546
MS.gene065947.t1 MTR_3g028590 74.425 391 75 4 1 369 1 388 0.0 545
MS.gene065947.t1 MTR_8g036880 74.332 374 81 5 1 369 35 398 0.0 519
MS.gene065947.t1 MTR_2g098070 72.973 370 89 4 1 369 2 361 4.82e-180 504
MS.gene065947.t1 MTR_8g107270 58.188 287 120 0 73 359 17 303 8.41e-128 370
MS.gene065947.t1 MTR_3g104500 40.136 294 165 6 70 357 30 318 7.66e-69 219
MS.gene065947.t1 MTR_6g091800 29.431 299 190 6 70 357 15 303 2.16e-34 129
MS.gene065947.t1 MTR_3g104600 30.990 313 174 10 70 357 31 326 6.93e-31 120
MS.gene065947.t1 MTR_6g091800 27.554 323 189 7 70 357 15 327 2.68e-30 119
MS.gene065947.t1 MTR_1g012030 29.568 301 185 10 65 360 132 410 4.74e-30 119
MS.gene065947.t1 MTR_1g012030 27.273 209 131 6 62 267 221 411 1.38e-14 75.1
MS.gene065947.t1 MTR_6g091800 30.159 252 159 4 70 314 15 256 3.16e-29 114
MS.gene065947.t1 MTR_6g091800 31.020 245 152 4 70 307 15 249 5.58e-29 113
MS.gene065947.t1 MTR_6g091800 27.597 308 179 6 70 343 15 312 5.82e-29 114
MS.gene065947.t1 MTR_3g107510 28.881 277 173 8 77 349 125 381 1.73e-28 115
MS.gene065947.t1 MTR_3g107510 28.571 196 120 5 75 267 216 394 9.16e-16 78.6
MS.gene065947.t1 MTR_7g068340 31.095 283 164 8 85 360 98 356 2.31e-26 108
MS.gene065947.t1 MTR_5g009500 28.660 321 175 12 74 360 38 338 2.94e-26 107
MS.gene065947.t1 MTR_5g009500 23.661 224 154 4 54 266 121 338 9.71e-11 62.8
MS.gene065947.t1 MTR_8g027975 29.195 298 174 10 73 344 50 336 1.60e-25 106
MS.gene065947.t1 MTR_8g027975 26.108 203 131 5 77 266 157 353 1.03e-11 65.9
MS.gene065947.t1 MTR_6g091800 27.899 276 158 5 70 314 15 280 2.66e-25 104
MS.gene065947.t1 MTR_6g091800 28.625 269 151 5 70 307 15 273 5.30e-25 103
MS.gene065947.t1 MTR_7g064980 28.571 308 201 8 64 360 15 314 5.56e-25 103
MS.gene065947.t1 MTR_4g114320 27.698 278 181 6 74 349 123 382 9.61e-25 104
MS.gene065947.t1 MTR_4g114320 28.502 207 126 5 60 263 204 391 8.10e-17 81.6
MS.gene065947.t1 MTR_6g034145 29.268 246 160 5 123 357 7 249 1.89e-24 101
MS.gene065947.t1 MTR_6g034145 27.317 205 133 7 65 263 55 249 1.82e-12 67.4
MS.gene065947.t1 MTR_6g092210 29.167 240 162 5 123 357 7 243 8.53e-24 99.4
MS.gene065947.t1 MTR_6g092210 25.628 199 138 4 65 263 55 243 2.60e-12 66.6
MS.gene065947.t1 MTR_6g091800 31.694 183 109 3 70 245 15 188 1.22e-23 97.1
MS.gene065947.t1 MTR_5g081150 27.419 310 205 8 60 360 23 321 2.32e-23 99.4
MS.gene065947.t1 MTR_2g436930 27.673 318 185 8 71 354 12 318 4.76e-23 98.6
MS.gene065947.t1 MTR_8g096050 28.723 282 177 9 74 349 227 490 2.27e-22 98.6
MS.gene065947.t1 MTR_4g119770 26.398 322 190 8 71 356 11 321 5.87e-22 95.5
MS.gene065947.t1 MTR_7g084400 28.772 285 170 8 74 349 213 473 6.14e-22 97.4
MS.gene065947.t1 MTR_4g016730 31.597 288 163 11 74 354 89 349 1.56e-21 95.1
MS.gene065947.t1 MTR_3g098460 28.622 283 176 10 74 349 205 468 2.84e-20 92.4
MS.gene065947.t1 MTR_6g091800 28.986 207 107 4 70 245 15 212 1.03e-19 87.0
MS.gene065947.t1 MTR_4g119770 26.923 260 154 6 71 305 11 259 2.43e-19 87.0
MS.gene065947.t1 MTR_3g074490 27.597 308 176 12 77 360 119 403 1.75e-18 86.7
MS.gene065947.t1 MTR_4g005330 24.497 298 183 7 73 349 65 341 2.91e-17 82.4
MS.gene065947.t1 MTR_6g091910 28.283 198 131 5 70 263 15 205 1.00e-16 78.6
MS.gene065947.t1 MTR_6g091910 27.083 192 130 5 175 357 15 205 3.39e-11 62.8
MS.gene065947.t1 MTR_4g005330 24.490 294 180 7 77 349 3 275 3.33e-16 78.6
MS.gene065947.t1 MTR_1g090330 23.308 266 192 5 74 335 14 271 8.68e-16 77.4
MS.gene065947.t1 MTR_1g090330 29.167 168 111 4 75 241 112 272 4.92e-14 72.4
MS.gene065947.t1 MTR_5g030530 26.962 293 194 10 73 354 7 290 1.18e-15 77.0
MS.gene065947.t1 MTR_7g066690 23.507 268 196 6 70 335 13 273 4.94e-15 75.5
MS.gene065947.t1 MTR_7g084400 30.102 196 111 5 74 263 207 382 5.79e-15 76.3
MS.gene065947.t1 MTR_3g100570 24.573 293 190 8 74 342 6 291 8.10e-15 74.7
MS.gene065947.t1 MTR_5g081150 30.201 149 95 3 60 204 23 166 1.23e-14 73.2
MS.gene065947.t1 MTR_5g081150 30.201 149 95 3 60 204 23 166 1.52e-14 73.2
MS.gene065947.t1 MTR_8g066975 24.573 293 180 8 73 344 7 279 1.15e-13 71.2
MS.gene065947.t1 MTR_4g018750 24.060 266 189 8 74 335 15 271 1.45e-13 70.9
MS.gene065947.t1 MTR_2g102010 24.476 286 199 6 73 354 16 288 1.50e-13 70.9
MS.gene065947.t1 MTR_2g436930 29.082 196 112 4 180 354 39 228 3.17e-13 68.9
MS.gene065947.t1 MTR_7g109135 22.408 299 209 8 61 349 9 294 8.21e-13 68.9
MS.gene065947.t1 MTR_8g090210 25.000 308 184 12 77 360 113 397 9.99e-13 69.3
MS.gene065947.t1 MTR_4g057850 25.155 322 168 12 76 365 27 307 1.23e-12 68.6
MS.gene065947.t1 MTR_4g012850 25.692 253 174 5 119 364 51 296 1.53e-12 68.2
MS.gene065947.t1 MTR_4g012850 26.432 227 150 6 36 260 75 286 4.06e-11 63.5
MS.gene065947.t1 MTR_4g018750 24.303 251 177 8 74 320 15 256 9.23e-12 65.5
MS.gene065947.t1 MTR_5g032420 21.782 303 195 8 74 342 6 300 7.86e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene065947.t1 AT4G28390 80.159 378 65 2 3 370 2 379 0.0 583
MS.gene065947.t1 AT4G28390 80.159 378 65 2 3 370 2 379 0.0 583
MS.gene065947.t1 AT5G13490 74.286 385 82 4 1 369 1 384 0.0 544
MS.gene065947.t1 AT5G13490 74.286 385 82 4 1 369 1 384 0.0 544
MS.gene065947.t1 AT3G08580 74.278 381 85 4 1 369 1 380 0.0 527
MS.gene065947.t1 AT3G08580 74.278 381 85 4 1 369 1 380 0.0 527
MS.gene065947.t1 AT5G17400 61.644 292 112 0 70 361 10 301 3.71e-132 380
MS.gene065947.t1 AT5G56450 38.284 303 168 7 70 360 30 325 3.93e-62 202
MS.gene065947.t1 AT4G26180 33.225 307 175 9 70 360 17 309 1.22e-33 127
MS.gene065947.t1 AT4G26180 33.564 289 163 8 70 343 17 291 2.76e-32 123
MS.gene065947.t1 AT2G37890 29.641 334 216 8 37 360 8 332 3.16e-32 124
MS.gene065947.t1 AT3G55640 28.814 295 193 7 73 358 37 323 1.68e-30 119
MS.gene065947.t1 AT3G55640 28.814 295 193 7 73 358 37 323 1.68e-30 119
MS.gene065947.t1 AT1G14560 29.893 281 171 6 85 349 38 308 4.08e-30 118
MS.gene065947.t1 AT1G14560 30.182 275 166 6 85 343 38 302 2.59e-29 115
MS.gene065947.t1 AT3G51870 32.979 282 160 10 85 360 102 360 2.18e-28 114
MS.gene065947.t1 AT3G53940 29.870 308 195 9 64 360 63 360 4.24e-28 113
MS.gene065947.t1 AT4G32400 30.605 281 169 9 74 349 114 373 1.50e-27 112
MS.gene065947.t1 AT4G32400 26.020 196 125 5 75 267 208 386 3.84e-12 67.4
MS.gene065947.t1 AT5G01500 33.212 274 158 11 85 354 130 382 3.66e-27 111
MS.gene065947.t1 AT4G01100 27.974 311 186 10 77 360 45 344 1.05e-24 103
MS.gene065947.t1 AT5G51050 29.643 280 174 7 74 349 223 483 8.08e-24 102
MS.gene065947.t1 AT4G01100 27.385 325 184 11 77 360 45 358 2.20e-23 100
MS.gene065947.t1 AT4G01100 27.213 305 184 10 83 360 1 294 1.03e-22 97.4
MS.gene065947.t1 AT4G01100 25.854 205 129 6 77 266 98 294 6.65e-12 66.2
MS.gene065947.t1 AT5G61810 27.857 280 174 7 74 349 65 320 1.92e-22 97.1
MS.gene065947.t1 AT5G48970 27.673 318 188 8 71 354 19 328 2.30e-22 97.1
MS.gene065947.t1 AT3G21390 26.349 315 195 7 71 354 16 324 4.08e-22 96.3
MS.gene065947.t1 AT3G20240 25.085 295 185 6 73 349 51 327 4.46e-22 96.3
MS.gene065947.t1 AT3G21390 26.349 315 195 7 71 354 38 346 8.51e-22 95.5
MS.gene065947.t1 AT3G55640 28.718 195 127 3 73 263 37 223 1.06e-21 93.6
MS.gene065947.t1 AT3G55640 28.718 195 127 3 73 263 37 223 1.06e-21 93.6
MS.gene065947.t1 AT5G61810 27.857 280 174 7 74 349 208 463 2.03e-21 95.9
MS.gene065947.t1 AT3G53940 29.630 216 138 4 64 274 63 269 1.15e-19 88.6
MS.gene065947.t1 AT1G14560 31.383 188 112 4 178 349 24 210 1.33e-19 87.0
MS.gene065947.t1 AT5G07320 26.296 270 173 7 83 349 218 464 1.81e-19 90.1
MS.gene065947.t1 AT5G48970 26.953 256 152 6 126 354 10 257 2.63e-16 78.6
MS.gene065947.t1 AT5G64970 27.458 295 170 11 77 349 138 410 2.44e-15 77.4
MS.gene065947.t1 AT3G54110 23.485 264 193 7 74 335 15 271 5.59e-15 75.1
MS.gene065947.t1 AT1G14140 24.820 278 192 7 64 335 8 274 7.20e-15 75.1
MS.gene065947.t1 AT5G64970 26.780 295 172 11 77 349 14 286 1.12e-14 74.3
MS.gene065947.t1 AT5G64970 27.778 288 164 11 77 342 138 403 2.12e-14 74.3
MS.gene065947.t1 AT5G58970 24.460 278 195 6 70 342 12 279 2.60e-14 73.2
MS.gene065947.t1 AT5G58970 27.919 197 123 7 175 357 12 203 7.51e-11 63.2
MS.gene065947.t1 AT1G78180 27.273 308 177 12 77 360 131 415 2.25e-13 71.2
MS.gene065947.t1 AT5G66380 27.805 205 124 6 172 364 5 197 1.79e-12 67.8
MS.gene065947.t1 AT4G27940 25.368 272 168 12 114 360 146 407 2.44e-11 65.1
MS.gene065947.t1 AT5G46800 24.830 294 202 8 73 354 7 293 2.60e-11 64.3
MS.gene065947.t1 AT5G46800 24.830 294 202 8 73 354 7 293 2.60e-11 64.3
MS.gene065947.t1 AT5G46800 24.830 294 202 8 73 354 7 293 2.60e-11 64.3
MS.gene065947.t1 AT5G58970 27.919 197 123 7 175 357 12 203 5.84e-11 63.2

Find 101 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCCTATTTGTCTATTCTT+TGG 0.187425 4.2:+31428712 MS.gene065947:CDS
GATAGGTGGACATTCTTATT+TGG 0.215860 4.2:+31427703 MS.gene065947:CDS
TGAGCATTTCATCCTGGTTT+TGG 0.229183 4.2:-31427958 None:intergenic
GACATTCTTATTTGGTTTCC+AGG 0.255723 4.2:+31427711 MS.gene065947:CDS
GTCTTGTTTGGAAAGAAATA+TGG 0.260053 4.2:+31429698 MS.gene065947:CDS
GGTGGTGAGAGGCAGTTTAA+TGG 0.306537 4.2:+31428782 MS.gene065947:CDS
GCAGGTGCTGGTGTGCTTGC+TGG 0.309350 4.2:+31429656 MS.gene065947:CDS
TATCACAATTGGTGCTGGTT+TGG 0.315135 4.2:+31429475 MS.gene065947:CDS
AATCCAAAGAATAGACAAAT+AGG 0.321819 4.2:-31428715 None:intergenic
CAGTCACCAATTCCCTTATA+TGG 0.327603 4.2:-31428001 None:intergenic
TGCTAGTTTCCTGTTGGGAT+GGG 0.328388 4.2:+31429451 MS.gene065947:CDS
AATTATACCCTCATCCTTCA+TGG 0.333517 4.2:-31428035 None:intergenic
TTAGTATCTGGTGGAGCTGC+TGG 0.333758 4.2:+31428680 MS.gene065947:CDS
AACATATTGCGTGCTGTTGC+AGG 0.340667 4.2:+31429638 MS.gene065947:CDS
TTCTTTGCTAGTTTCCTGTT+GGG 0.343196 4.2:+31429446 MS.gene065947:CDS
CCTATCTTCTGAGCAATTGA+TGG 0.352718 4.2:-31427686 None:intergenic
AGAAGAATGATGATGACTTC+TGG 0.353166 4.2:+31429524 MS.gene065947:CDS
AAGCTGCAGCTGGTCTTGTT+TGG 0.353609 4.2:+31429686 MS.gene065947:CDS
TTACAGTCTGATGGCATTGC+TGG 0.359420 4.2:+31428830 MS.gene065947:CDS
AGCAATTTAATTCGTTCAAT+AGG 0.374655 4.2:-31427932 None:intergenic
GGCTACTGGAAGTGGTTTGC+TGG 0.374984 4.2:+31428653 MS.gene065947:CDS
TTTGGAAAGAAATATGGTTC+TGG 0.380189 4.2:+31429704 MS.gene065947:CDS
CTTTGCGTCATTTGCCAAAC+GGG 0.396020 4.2:-31428747 None:intergenic
ACTAATGGAGTGCAGACTTC+TGG 0.400993 4.2:+31427782 MS.gene065947:CDS
TTGCTAGTTTCCTGTTGGGA+TGG 0.404433 4.2:+31429450 MS.gene065947:CDS
AGATGTTGAATCCACGATAA+AGG 0.405874 4.2:-31428853 None:intergenic
CCATCAATTGCTCAGAAGAT+AGG 0.406150 4.2:+31427686 MS.gene065947:CDS
GGATTATGCCCGTACCCGTT+TGG 0.408041 4.2:+31428733 MS.gene065947:CDS
CTGAAACCAGTTGTCTTGGT+TGG 0.408866 4.2:+31428929 MS.gene065947:CDS
TCTGAGCCATATAAGGGAAT+TGG 0.421286 4.2:+31427995 MS.gene065947:CDS
TCTTCTACGCACAGTGTCAA+TGG 0.421926 4.2:-31429507 None:intergenic
GTTAGAGTTGGCGCTAAGCC+TGG 0.422723 4.2:-31427729 None:intergenic
CAGGTTATCTGAGCCATATA+AGG 0.423186 4.2:+31427988 MS.gene065947:CDS
TAGTATCTGGTGGAGCTGCT+GGG 0.429291 4.2:+31428681 MS.gene065947:CDS
GTGTATCGTGGTCTTTACTT+TGG 0.436559 4.2:+31428893 MS.gene065947:CDS
TGGGGTATCACAATTGGTGC+TGG 0.437821 4.2:+31429470 MS.gene065947:CDS
TTTGCTGGGAATTTAGTATC+TGG 0.438983 4.2:+31428668 MS.gene065947:CDS
CCAAACCATCGTTGCAAAAG+AGG 0.440517 4.2:+31429583 MS.gene065947:CDS
TGGAGTGCAGACTTCTGGTT+TGG 0.442748 4.2:+31427787 MS.gene065947:CDS
TGACGCAAAGGCTGCAAAGA+AGG 0.445560 4.2:+31428760 MS.gene065947:CDS
TTGGGATGGGGTATCACAAT+TGG 0.445710 4.2:+31429464 MS.gene065947:CDS
TTTCTTTGCTAGTTTCCTGT+TGG 0.448910 4.2:+31429445 MS.gene065947:CDS
CGTCATTTGCCAAACGGGTA+CGG 0.451569 4.2:-31428742 None:intergenic
TTCTCTGAAACCAGTTGTCT+TGG 0.454364 4.2:+31428925 MS.gene065947:CDS
ACGTCCGGTACTTTCACTAA+TGG 0.459227 4.2:+31427767 MS.gene065947:CDS
CCAGCGGGTCCTTTCTCTGC+TGG 0.461085 4.2:-31427848 None:intergenic
TTGCGTGCTGTTGCAGGTGC+TGG 0.462273 4.2:+31429644 MS.gene065947:CDS
AAGAAATATGGTTCTGGCGG+CGG 0.463431 4.2:+31429710 MS.gene065947:CDS
GGTCCTTTCTCTGCTGGAGA+AGG 0.466249 4.2:-31427842 None:intergenic
AAACCAGTTGTCTTGGTTGG+TGG 0.469067 4.2:+31428932 MS.gene065947:CDS
TTGTGATACCCCATCCCAAC+AGG 0.471309 4.2:-31429460 None:intergenic
CACAGTGTCAATGGGATAAG+AGG 0.473276 4.2:-31429498 None:intergenic
ATTCAGATTATCACCTGAGT+AGG 0.479406 4.2:-31428100 None:intergenic
GCTAGTTTCCTGTTGGGATG+GGG 0.479609 4.2:+31429452 MS.gene065947:CDS
TGTCTTGGTTGGTGGGATGC+AGG 0.479765 4.2:+31428940 MS.gene065947:CDS
ATCCCACCAACCAAGACAAC+TGG 0.485200 4.2:-31428935 None:intergenic
AGGTTATCTGAGCCATATAA+GGG 0.488890 4.2:+31427989 MS.gene065947:CDS
CCTTTGCGTCATTTGCCAAA+CGG 0.490345 4.2:-31428748 None:intergenic
GTCATTTGCCAAACGGGTAC+GGG 0.492794 4.2:-31428741 None:intergenic
ACAGAGATGGCTACTGGAAG+TGG 0.495428 4.2:+31428645 MS.gene065947:CDS
GCTGGGAATTTAGTATCTGG+TGG 0.499128 4.2:+31428671 MS.gene065947:CDS
AGCAATGCCATCAGACTGTA+AGG 0.504971 4.2:-31428828 None:intergenic
TACAGGAATTACTCTACGTC+CGG 0.508953 4.2:+31427752 MS.gene065947:CDS
GTCCCTTCTCCAGCAGAGAA+AGG 0.510593 4.2:+31427839 MS.gene065947:CDS
CTTCTACGCACAGTGTCAAT+GGG 0.512712 4.2:-31429506 None:intergenic
GTAATTCCTGTAGTTAGAGT+TGG 0.521037 4.2:-31427741 None:intergenic
AACCAGTTGTCTTGGTTGGT+GGG 0.523148 4.2:+31428933 MS.gene065947:CDS
TGCTCGAACCATGAAGGATG+AGG 0.524575 4.2:+31428027 MS.gene065947:CDS
TGGTTATGACAAGCTGCAGC+TGG 0.524956 4.2:+31429676 MS.gene065947:CDS
GGCATTGCTGGCCTTTATCG+TGG 0.542365 4.2:+31428842 MS.gene065947:CDS
GTCCTTTCTCTGCTGGAGAA+GGG 0.549707 4.2:-31427841 None:intergenic
GCTACTGGAAGTGGTTTGCT+GGG 0.556481 4.2:+31428654 MS.gene065947:CDS
GGATTCAACATCTCGTGTGT+TGG 0.556905 4.2:+31428863 MS.gene065947:CDS
ATGAAATGCTCAAAAGTGGC+AGG 0.556927 4.2:+31427969 MS.gene065947:CDS
AGTATCTGGTGGAGCTGCTG+GGG 0.568550 4.2:+31428682 MS.gene065947:CDS
TTAGCGCCAACTCTAACTAC+AGG 0.570302 4.2:+31427735 MS.gene065947:CDS
TTCAGATTATCACCTGAGTA+GGG 0.571644 4.2:-31428099 None:intergenic
GACGCAAAGGCTGCAAAGAA+GGG 0.572621 4.2:+31428761 MS.gene065947:CDS
CCAGCAGAGAAAGGACCCGC+TGG 0.578869 4.2:+31427848 MS.gene065947:CDS
TGAGAGGCAGTTTAATGGAA+TGG 0.581022 4.2:+31428787 MS.gene065947:CDS
CAAACCATCGTTGCAAAAGA+GGG 0.586013 4.2:+31429584 MS.gene065947:CDS
CAGGATGAAATGCTCAAAAG+TGG 0.586952 4.2:+31427965 MS.gene065947:CDS
AAGAAAACCTTACAGTCTGA+TGG 0.592392 4.2:+31428821 MS.gene065947:CDS
TAGGCTGCAATCATGGCCGA+TGG 0.596371 4.2:+31427653 None:intergenic
CACTCCATTAGTGAAAGTAC+CGG 0.601548 4.2:-31427771 None:intergenic
TAAATTGCTTATCCAAAACC+AGG 0.601587 4.2:+31427946 MS.gene065947:CDS
GGTATAATTGCTCTGTGGAG+AGG 0.606231 4.2:+31428049 MS.gene065947:CDS
GCTCGAACCATGAAGGATGA+GGG 0.608674 4.2:+31428028 MS.gene065947:CDS
TGATGGATGATGTGGTCCAT+CGG 0.611029 4.2:-31427669 None:intergenic
GGAAAGAAATATGGTTCTGG+CGG 0.618520 4.2:+31429707 MS.gene065947:CDS
TCAATTGCTCAGAAGATAGG+TGG 0.621358 4.2:+31427689 MS.gene065947:CDS
CTGCAAAGAAGGGTGGTGAG+AGG 0.621915 4.2:+31428771 MS.gene065947:CDS
AGAATAGACAAATAGGAGTG+AGG 0.622653 4.2:-31428708 None:intergenic
CCGTTTGGCAAATGACGCAA+AGG 0.622778 4.2:+31428748 MS.gene065947:CDS
TATCAGATACTTCCCTACTC+AGG 0.622842 4.2:+31428087 MS.gene065947:CDS
TGAGCAATTGATGGATGATG+TGG 0.652110 4.2:-31427677 None:intergenic
ATAGACAAATAGGAGTGAGG+AGG 0.664089 4.2:-31428705 None:intergenic
GTTGGAATTATAGTGTATCG+TGG 0.683295 4.2:+31428881 MS.gene065947:CDS
GCAAAGGCTGCAAAGAAGGG+TGG 0.689413 4.2:+31428764 MS.gene065947:CDS
ATGAGGGTATAATTGCTCTG+TGG 0.733403 4.2:+31428044 MS.gene065947:CDS
GGGACAGTAAATGACGACAG+AGG 0.743353 4.2:-31427821 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATGAAATTAGAAATTAA+AGG - chr4.2:31428559-31428578 None:intergenic 15.0%
!! ATGAACATTACTAAAATAAT+TGG - chr4.2:31429382-31429401 None:intergenic 15.0%
!! TGAACATTACTAAAATAATT+GGG - chr4.2:31429381-31429400 None:intergenic 15.0%
!!! TGTACAAAATTTGATTTTAA+CGG + chr4.2:31428965-31428984 MS.gene065947:intron 15.0%
!! GAACATTACTAAAATAATTG+GGG - chr4.2:31429380-31429399 None:intergenic 20.0%
!! TTGAAACTTTAATTGAACTA+GGG + chr4.2:31429319-31429338 MS.gene065947:intron 20.0%
!!! AATTTCATTCTTTTCCTTTT+AGG + chr4.2:31428568-31428587 MS.gene065947:intron 20.0%
!!! AATTTTAAAAAAGACAGAGA+TGG + chr4.2:31428632-31428651 MS.gene065947:CDS 20.0%
!!! ATTGAGATTAGCTTATATTT+TGG + chr4.2:31428379-31428398 MS.gene065947:intron 20.0%
!!! CATCATTTTCTTATTATCTA+TGG + chr4.2:31429399-31429418 MS.gene065947:intron 20.0%
!!! GGAATAATTTTAATCTAACA+CGG - chr4.2:31429016-31429035 None:intergenic 20.0%
!!! GTACAAAATTTGATTTTAAC+GGG + chr4.2:31428966-31428985 MS.gene065947:intron 20.0%
!!! TAAAATTATTCCTCTTTTGT+TGG + chr4.2:31429024-31429043 MS.gene065947:intron 20.0%
!!! TTTTAATAGAAAGATGTCTA+GGG - chr4.2:31429264-31429283 None:intergenic 20.0%
! AAATGCAAGGATACAAAAAT+AGG - chr4.2:31428428-31428447 None:intergenic 25.0%
! AATCCAAAGAATAGACAAAT+AGG - chr4.2:31428718-31428737 None:intergenic 25.0%
! AGAAATTTGTGGAAATCTAA+TGG + chr4.2:31428226-31428245 MS.gene065947:intron 25.0%
! AGCAATTTAATTCGTTCAAT+AGG - chr4.2:31427935-31427954 None:intergenic 25.0%
! ATAAGTTATATGCGTTGTAA+GGG + chr4.2:31428171-31428190 MS.gene065947:intron 25.0%
! CTAAAAGAAAGTCAAAATTG+TGG - chr4.2:31429357-31429376 None:intergenic 25.0%
! CTTGAAACTTTAATTGAACT+AGG + chr4.2:31429318-31429337 MS.gene065947:intron 25.0%
! TTAGATTTCCACAAATTTCT+TGG - chr4.2:31428226-31428245 None:intergenic 25.0%
!! AGAAAATGTTTTGTATGTCT+AGG - chr4.2:31428456-31428475 None:intergenic 25.0%
!! GAAAATGTTTTGTATGTCTA+GGG - chr4.2:31428455-31428474 None:intergenic 25.0%
!!! ACTTTGATTTTATGTTTGTC+AGG + chr4.2:31429051-31429070 MS.gene065947:intron 25.0%
!!! AGATTAGCTTATATTTTGGA+AGG + chr4.2:31428383-31428402 MS.gene065947:intron 25.0%
!!! ATTTTTGATCGATTTTCTGA+TGG + chr4.2:31427871-31427890 MS.gene065947:CDS 25.0%
!!! GAAAAAGGTGTTTAATAGAA+AGG - chr4.2:31429242-31429261 None:intergenic 25.0%
!!! GATTAGCTTATATTTTGGAA+GGG + chr4.2:31428384-31428403 MS.gene065947:intron 25.0%
!!! GTTTTAATAGAAAGATGTCT+AGG - chr4.2:31429265-31429284 None:intergenic 25.0%
!!! TTTGATTTTAACGGGAAATA+TGG + chr4.2:31428974-31428993 MS.gene065947:intron 25.0%
!!! TTTTTGATCGATTTTCTGAT+GGG + chr4.2:31427872-31427891 MS.gene065947:CDS 25.0%
AAAATCGATCAAAAATCCAG+CGG - chr4.2:31427867-31427886 None:intergenic 30.0%
ACTCCTATTTGTCTATTCTT+TGG + chr4.2:31428712-31428731 MS.gene065947:CDS 30.0%
CAAAGTAAAACCAACAAAAG+AGG - chr4.2:31429037-31429056 None:intergenic 30.0%
CATAAGTTATATGCGTTGTA+AGG + chr4.2:31428170-31428189 MS.gene065947:intron 30.0%
CTTACAACGCATATAACTTA+TGG - chr4.2:31428172-31428191 None:intergenic 30.0%
GTGTTAAACCAAGAAATTTG+TGG + chr4.2:31428215-31428234 MS.gene065947:intron 30.0%
TAAATTGCTTATCCAAAACC+AGG + chr4.2:31427946-31427965 MS.gene065947:CDS 30.0%
TTAACACAATATACACTTCG+AGG - chr4.2:31428202-31428221 None:intergenic 30.0%
TTTAGCAATCTATGATGTCT+AGG - chr4.2:31429298-31429317 None:intergenic 30.0%
TTTGGAAAGAAATATGGTTC+TGG + chr4.2:31429704-31429723 MS.gene065947:CDS 30.0%
! ATGGTACTTTTCTTGTTAAC+AGG + chr4.2:31429418-31429437 MS.gene065947:intron 30.0%
! CTATGATGTCTAGGATATAA+AGG - chr4.2:31429289-31429308 None:intergenic 30.0%
! TATAACACATTACTGATTGC+AGG - chr4.2:31429213-31429232 None:intergenic 30.0%
!! CTTTTAAGGATTACTTCAAG+AGG + chr4.2:31428603-31428622 MS.gene065947:CDS 30.0%
!! GTCTTGTTTGGAAAGAAATA+TGG + chr4.2:31429698-31429717 MS.gene065947:CDS 30.0%
AAAAACTGTCACTGTGATTC+CGG + chr4.2:31428294-31428313 MS.gene065947:intron 35.0%
AAATCGATCAAAAATCCAGC+GGG - chr4.2:31427866-31427885 None:intergenic 35.0%
AAGAAAACCTTACAGTCTGA+TGG + chr4.2:31428821-31428840 MS.gene065947:CDS 35.0%
AATTATACCCTCATCCTTCA+TGG - chr4.2:31428038-31428057 None:intergenic 35.0%
AGAAAGATGTCTAGGGAAAA+AGG - chr4.2:31429257-31429276 None:intergenic 35.0%
AGAATAGACAAATAGGAGTG+AGG - chr4.2:31428711-31428730 None:intergenic 35.0%
AGATGTTGAATCCACGATAA+AGG - chr4.2:31428856-31428875 None:intergenic 35.0%
AGGTTATCTGAGCCATATAA+GGG + chr4.2:31427989-31428008 MS.gene065947:CDS 35.0%
ATTCAGATTATCACCTGAGT+AGG - chr4.2:31428103-31428122 None:intergenic 35.0%
GATAGGTGGACATTCTTATT+TGG + chr4.2:31427703-31427722 MS.gene065947:CDS 35.0%
GTAATTCCTGTAGTTAGAGT+TGG - chr4.2:31427744-31427763 None:intergenic 35.0%
GTTGGAATTATAGTGTATCG+TGG + chr4.2:31428881-31428900 MS.gene065947:CDS 35.0%
TGTGGTGTTTGTATAAGTAG+AGG - chr4.2:31428271-31428290 None:intergenic 35.0%
TTCAGATTATCACCTGAGTA+GGG - chr4.2:31428102-31428121 None:intergenic 35.0%
TTTGCTGGGAATTTAGTATC+TGG + chr4.2:31428668-31428687 MS.gene065947:CDS 35.0%
! ACAGTGACAGTTTTTGTATG+TGG - chr4.2:31428289-31428308 None:intergenic 35.0%
! AGAAGAATGATGATGACTTC+TGG + chr4.2:31429524-31429543 MS.gene065947:CDS 35.0%
! GACATTCTTATTTGGTTTCC+AGG + chr4.2:31427711-31427730 MS.gene065947:CDS 35.0%
! GGTACTAAGTCTCTTTTCAA+AGG + chr4.2:31429605-31429624 MS.gene065947:CDS 35.0%
! TTCTTTGCTAGTTTCCTGTT+GGG + chr4.2:31429446-31429465 MS.gene065947:CDS 35.0%
! TTTCCATGTGTTGTCTTGTT+TGG + chr4.2:31428328-31428347 MS.gene065947:intron 35.0%
! TTTCTTTGCTAGTTTCCTGT+TGG + chr4.2:31429445-31429464 MS.gene065947:CDS 35.0%
!! CTCTTTTGGTGATTGAATGT+TGG + chr4.2:31428510-31428529 MS.gene065947:intron 35.0%
!! TTCCATGTGTTGTCTTGTTT+GGG + chr4.2:31428329-31428348 MS.gene065947:intron 35.0%
AAAAAGACAGAGATGGCTAC+TGG + chr4.2:31428639-31428658 MS.gene065947:CDS 40.0%
AACCCAAACAAGACAACACA+TGG - chr4.2:31428334-31428353 None:intergenic 40.0%
AGCAAAGTTCAGAGCCTAAA+AGG - chr4.2:31428585-31428604 None:intergenic 40.0%
ATAGACAAATAGGAGTGAGG+AGG - chr4.2:31428708-31428727 None:intergenic 40.0%
ATGAAATGCTCAAAAGTGGC+AGG + chr4.2:31427969-31427988 MS.gene065947:CDS 40.0%
CAAACCATCGTTGCAAAAGA+GGG + chr4.2:31429584-31429603 MS.gene065947:CDS 40.0%
CAGGATGAAATGCTCAAAAG+TGG + chr4.2:31427965-31427984 MS.gene065947:CDS 40.0%
CAGGTTATCTGAGCCATATA+AGG + chr4.2:31427988-31428007 MS.gene065947:CDS 40.0%
CAGTCACCAATTCCCTTATA+TGG - chr4.2:31428004-31428023 None:intergenic 40.0%
CATGGAAAATCTCATGACAC+CGG - chr4.2:31428316-31428335 None:intergenic 40.0%
CCATCAATTGCTCAGAAGAT+AGG + chr4.2:31427686-31427705 MS.gene065947:CDS 40.0%
CCTATCTTCTGAGCAATTGA+TGG - chr4.2:31427689-31427708 None:intergenic 40.0%
GTGTATCGTGGTCTTTACTT+TGG + chr4.2:31428893-31428912 MS.gene065947:CDS 40.0%
TACAGGAATTACTCTACGTC+CGG + chr4.2:31427752-31427771 MS.gene065947:CDS 40.0%
TATCAGATACTTCCCTACTC+AGG + chr4.2:31428087-31428106 MS.gene065947:CDS 40.0%
TCAATTGCTCAGAAGATAGG+TGG + chr4.2:31427689-31427708 MS.gene065947:CDS 40.0%
TCTGAGCCATATAAGGGAAT+TGG + chr4.2:31427995-31428014 MS.gene065947:CDS 40.0%
TGAGAGGCAGTTTAATGGAA+TGG + chr4.2:31428787-31428806 MS.gene065947:CDS 40.0%
TGTCTAGGGATGTAAATGCA+AGG - chr4.2:31428441-31428460 None:intergenic 40.0%
! ATGAGGGTATAATTGCTCTG+TGG + chr4.2:31428044-31428063 MS.gene065947:CDS 40.0%
! CACTCCATTAGTGAAAGTAC+CGG - chr4.2:31427774-31427793 None:intergenic 40.0%
! CACTTTTGAGCATTTCATCC+TGG - chr4.2:31427967-31427986 None:intergenic 40.0%
! GATGCATGTGAATGCTCTTT+TGG + chr4.2:31428496-31428515 MS.gene065947:intron 40.0%
! TGAGCAATTGATGGATGATG+TGG - chr4.2:31427680-31427699 None:intergenic 40.0%
! TGAGCATTTCATCCTGGTTT+TGG - chr4.2:31427961-31427980 None:intergenic 40.0%
!! AAGTCTCTTTTCAAAGGTGC+TGG + chr4.2:31429611-31429630 MS.gene065947:CDS 40.0%
!! GGAAAGAAATATGGTTCTGG+CGG + chr4.2:31429707-31429726 MS.gene065947:CDS 40.0%
!! TATCACAATTGGTGCTGGTT+TGG + chr4.2:31429475-31429494 MS.gene065947:CDS 40.0%
!! TTCTCTGAAACCAGTTGTCT+TGG + chr4.2:31428925-31428944 MS.gene065947:CDS 40.0%
!! TTGATCGATTTTCTGATGGG+AGG + chr4.2:31427875-31427894 MS.gene065947:CDS 40.0%
!!! GGCTCTGAACTTTGCTTTTA+AGG + chr4.2:31428589-31428608 MS.gene065947:intron 40.0%
AAACCAGTTGTCTTGGTTGG+TGG + chr4.2:31428932-31428951 MS.gene065947:CDS 45.0%
AACCAGTTGTCTTGGTTGGT+GGG + chr4.2:31428933-31428952 MS.gene065947:CDS 45.0%
ACGTCCGGTACTTTCACTAA+TGG + chr4.2:31427767-31427786 MS.gene065947:CDS 45.0%
ACTAATGGAGTGCAGACTTC+TGG + chr4.2:31427782-31427801 MS.gene065947:CDS 45.0%
AGCAATGCCATCAGACTGTA+AGG - chr4.2:31428831-31428850 None:intergenic 45.0%
CACAGTGTCAATGGGATAAG+AGG - chr4.2:31429501-31429520 None:intergenic 45.0%
CCAAACCATCGTTGCAAAAG+AGG + chr4.2:31429583-31429602 MS.gene065947:CDS 45.0%
CCTTTGCGTCATTTGCCAAA+CGG - chr4.2:31428751-31428770 None:intergenic 45.0%
CTGAAACCAGTTGTCTTGGT+TGG + chr4.2:31428929-31428948 MS.gene065947:CDS 45.0%
CTTCTACGCACAGTGTCAAT+GGG - chr4.2:31429509-31429528 None:intergenic 45.0%
CTTTGCGTCATTTGCCAAAC+GGG - chr4.2:31428750-31428769 None:intergenic 45.0%
GCTGGGAATTTAGTATCTGG+TGG + chr4.2:31428671-31428690 MS.gene065947:CDS 45.0%
GGATTCAACATCTCGTGTGT+TGG + chr4.2:31428863-31428882 MS.gene065947:CDS 45.0%
GGTATAATTGCTCTGTGGAG+AGG + chr4.2:31428049-31428068 MS.gene065947:CDS 45.0%
TCTTCTACGCACAGTGTCAA+TGG - chr4.2:31429510-31429529 None:intergenic 45.0%
TTACAGTCTGATGGCATTGC+TGG + chr4.2:31428830-31428849 MS.gene065947:CDS 45.0%
TTAGCGCCAACTCTAACTAC+AGG + chr4.2:31427735-31427754 MS.gene065947:CDS 45.0%
! AACATATTGCGTGCTGTTGC+AGG + chr4.2:31429638-31429657 MS.gene065947:CDS 45.0%
! AGTACCCTCTTTTGCAACGA+TGG - chr4.2:31429591-31429610 None:intergenic 45.0%
! CCTCTTTTGCAACGATGGTT+TGG - chr4.2:31429586-31429605 None:intergenic 45.0%
! CTGTTTTGCTCGAACCATGA+AGG + chr4.2:31428021-31428040 MS.gene065947:CDS 45.0%
! TGATGGATGATGTGGTCCAT+CGG - chr4.2:31427672-31427691 None:intergenic 45.0%
! TGCTAGTTTCCTGTTGGGAT+GGG + chr4.2:31429451-31429470 MS.gene065947:CDS 45.0%
! TTGCTAGTTTCCTGTTGGGA+TGG + chr4.2:31429450-31429469 MS.gene065947:CDS 45.0%
! TTGGGATGGGGTATCACAAT+TGG + chr4.2:31429464-31429483 MS.gene065947:CDS 45.0%
!! AAGAAATATGGTTCTGGCGG+CGG + chr4.2:31429710-31429729 MS.gene065947:CDS 45.0%
ACAGAGATGGCTACTGGAAG+TGG + chr4.2:31428645-31428664 MS.gene065947:CDS 50.0%
ATCCCACCAACCAAGACAAC+TGG - chr4.2:31428938-31428957 None:intergenic 50.0%
CCGTTTGGCAAATGACGCAA+AGG + chr4.2:31428748-31428767 MS.gene065947:CDS 50.0%
CGTCATTTGCCAAACGGGTA+CGG - chr4.2:31428745-31428764 None:intergenic 50.0%
GACGCAAAGGCTGCAAAGAA+GGG + chr4.2:31428761-31428780 MS.gene065947:CDS 50.0%
GCTCGAACCATGAAGGATGA+GGG + chr4.2:31428028-31428047 MS.gene065947:CDS 50.0%
GGGACAGTAAATGACGACAG+AGG - chr4.2:31427824-31427843 None:intergenic 50.0%
GGTGGTGAGAGGCAGTTTAA+TGG + chr4.2:31428782-31428801 MS.gene065947:CDS 50.0%
GTCATTTGCCAAACGGGTAC+GGG - chr4.2:31428744-31428763 None:intergenic 50.0%
GTCCTTTCTCTGCTGGAGAA+GGG - chr4.2:31427844-31427863 None:intergenic 50.0%
TAGTATCTGGTGGAGCTGCT+GGG + chr4.2:31428681-31428700 MS.gene065947:CDS 50.0%
TGACGCAAAGGCTGCAAAGA+AGG + chr4.2:31428760-31428779 MS.gene065947:CDS 50.0%
TGCTCGAACCATGAAGGATG+AGG + chr4.2:31428027-31428046 MS.gene065947:CDS 50.0%
TGGTTATGACAAGCTGCAGC+TGG + chr4.2:31429676-31429695 MS.gene065947:CDS 50.0%
TTAGTATCTGGTGGAGCTGC+TGG + chr4.2:31428680-31428699 MS.gene065947:CDS 50.0%
TTGTGATACCCCATCCCAAC+AGG - chr4.2:31429463-31429482 None:intergenic 50.0%
! GCTAGTTTCCTGTTGGGATG+GGG + chr4.2:31429452-31429471 MS.gene065947:CDS 50.0%
! TGGAGTGCAGACTTCTGGTT+TGG + chr4.2:31427787-31427806 MS.gene065947:CDS 50.0%
! TGGGGTATCACAATTGGTGC+TGG + chr4.2:31429470-31429489 MS.gene065947:CDS 50.0%
!! AAGCTGCAGCTGGTCTTGTT+TGG + chr4.2:31429686-31429705 MS.gene065947:CDS 50.0%
!! GCTACTGGAAGTGGTTTGCT+GGG + chr4.2:31428654-31428673 MS.gene065947:CDS 50.0%
AGTATCTGGTGGAGCTGCTG+GGG + chr4.2:31428682-31428701 MS.gene065947:CDS 55.0%
CTGCAAAGAAGGGTGGTGAG+AGG + chr4.2:31428771-31428790 MS.gene065947:CDS 55.0%
GCAAAGGCTGCAAAGAAGGG+TGG + chr4.2:31428764-31428783 MS.gene065947:CDS 55.0%
GGATTATGCCCGTACCCGTT+TGG + chr4.2:31428733-31428752 MS.gene065947:CDS 55.0%
GGTCCTTTCTCTGCTGGAGA+AGG - chr4.2:31427845-31427864 None:intergenic 55.0%
GTCCCTTCTCCAGCAGAGAA+AGG + chr4.2:31427839-31427858 MS.gene065947:CDS 55.0%
GTTAGAGTTGGCGCTAAGCC+TGG - chr4.2:31427732-31427751 None:intergenic 55.0%
! GGCTACTGGAAGTGGTTTGC+TGG + chr4.2:31428653-31428672 MS.gene065947:CDS 55.0%
!! GGCATTGCTGGCCTTTATCG+TGG + chr4.2:31428842-31428861 MS.gene065947:CDS 55.0%
!! TGTCTTGGTTGGTGGGATGC+AGG + chr4.2:31428940-31428959 MS.gene065947:CDS 55.0%
! TTGCGTGCTGTTGCAGGTGC+TGG + chr4.2:31429644-31429663 MS.gene065947:CDS 60.0%
CCAGCAGAGAAAGGACCCGC+TGG + chr4.2:31427848-31427867 MS.gene065947:CDS 65.0%
CCAGCGGGTCCTTTCTCTGC+TGG - chr4.2:31427851-31427870 None:intergenic 65.0%
!! GCAGGTGCTGGTGTGCTTGC+TGG + chr4.2:31429656-31429675 MS.gene065947:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 31427665 31429736 31427665 ID=MS.gene065947
chr4.2 mRNA 31427665 31429736 31427665 ID=MS.gene065947.t1;Parent=MS.gene065947
chr4.2 exon 31427665 31428108 31427665 ID=MS.gene065947.t1.exon1;Parent=MS.gene065947.t1
chr4.2 CDS 31427665 31428108 31427665 ID=cds.MS.gene065947.t1;Parent=MS.gene065947.t1
chr4.2 exon 31428590 31428961 31428590 ID=MS.gene065947.t1.exon2;Parent=MS.gene065947.t1
chr4.2 CDS 31428590 31428961 31428590 ID=cds.MS.gene065947.t1;Parent=MS.gene065947.t1
chr4.2 exon 31429440 31429736 31429440 ID=MS.gene065947.t1.exon3;Parent=MS.gene065947.t1
chr4.2 CDS 31429440 31429736 31429440 ID=cds.MS.gene065947.t1;Parent=MS.gene065947.t1
Gene Sequence

>MS.gene065947

ATGGCCGATGGACCACATCATCCATCAATTGCTCAGAAGATAGGTGGACATTCTTATTTGGTTTCCAGGCTTAGCGCCAACTCTAACTACAGGAATTACTCTACGTCCGGTACTTTCACTAATGGAGTGCAGACTTCTGGTTTGGCACTTGCGTCACCTCTGTCGTCATTTACTGTCCCTTCTCCAGCAGAGAAAGGACCCGCTGGATTTTTGATCGATTTTCTGATGGGAGGAGTGTCTGCTGCTGTGTCAAAGACAGCTGCTGCTCCTATTGAACGAATTAAATTGCTTATCCAAAACCAGGATGAAATGCTCAAAAGTGGCAGGTTATCTGAGCCATATAAGGGAATTGGTGACTGTTTTGCTCGAACCATGAAGGATGAGGGTATAATTGCTCTGTGGAGAGGCAATACTGCTAATGTTATCAGATACTTCCCTACTCAGGTGATAATCTGAATTTTTTTCTTGTAAGATATTTTCTATTTTGACATATTCTTACAGCATCCATAAGTTATATGCGTTGTAAGGGTTGTTCCTCGAAGTGTATATTGTGTTAAACCAAGAAATTTGTGGAAATCTAATGGTTGAATATTCTTTTACTGACCTCTACTTATACAAACACCACATACAAAAACTGTCACTGTGATTCCGGTGTCATGAGATTTTCCATGTGTTGTCTTGTTTGGGTTTTGTTCATGCTGTCTTATTATATATATTGAGATTAGCTTATATTTTGGAAGGGAAAAGATTTTATATAATACCTATTTTTGTATCCTTGCATTTACATCCCTAGACATACAAAACATTTTCTCTCTTTTTTTATCTGATTTTGATGCATGTGAATGCTCTTTTGGTGATTGAATGTTGGTTCTGTAGTTTCTGCGAGCTGTTCCTTTAATTTCTAATTTCATTCTTTTCCTTTTAGGCTCTGAACTTTGCTTTTAAGGATTACTTCAAGAGGCTTTTCAATTTTAAAAAAGACAGAGATGGCTACTGGAAGTGGTTTGCTGGGAATTTAGTATCTGGTGGAGCTGCTGGGGCCTCCTCACTCCTATTTGTCTATTCTTTGGATTATGCCCGTACCCGTTTGGCAAATGACGCAAAGGCTGCAAAGAAGGGTGGTGAGAGGCAGTTTAATGGAATGGTTGATGTTTACAAGAAAACCTTACAGTCTGATGGCATTGCTGGCCTTTATCGTGGATTCAACATCTCGTGTGTTGGAATTATAGTGTATCGTGGTCTTTACTTTGGAATGTATGATTCTCTGAAACCAGTTGTCTTGGTTGGTGGGATGCAGGTATGTACAAAATTTGATTTTAACGGGAAATATGGTGTTTCTTAATCTGTTCCGTGTTAGATTAAAATTATTCCTCTTTTGTTGGTTTTACTTTGATTTTATGTTTGTCAGGCAATATTTTGTTGATTGTAAACGATTAGCTTGAATATTTAATTTTTTATGTCTTAAATATAATTTGAAAATGATGCTTTTAGAATGCTAATCTAGTTTATTAGCACATTATCTGTAAGCATGTTAGTATTCAGTTACCTGCAATCAGTAATGTGTTATATATCTTCCTTTCTATTAAACACCTTTTTCCCTAGACATCTTTCTATTAAAACACCTTTATATCCTAGACATCATAGATTGCTAAACTTGAAACTTTAATTGAACTAGGGAAATTTGTTCTTCCACAATTTTGACTTTCTTTTAGCCCCAATTATTTTAGTAATGTTCATCATTTTCTTATTATCTATGGTACTTTTCTTGTTAACAGGATAGTTTCTTTGCTAGTTTCCTGTTGGGATGGGGTATCACAATTGGTGCTGGTTTGGCCTCTTATCCCATTGACACTGTGCGTAGAAGAATGATGATGACTTCTGGAGAAGCTGTGAAGTATAACAGCTCTTTGCATGCATTCCAAACCATCGTTGCAAAAGAGGGTACTAAGTCTCTTTTCAAAGGTGCTGGTGCAAACATATTGCGTGCTGTTGCAGGTGCTGGTGTGCTTGCTGGTTATGACAAGCTGCAGCTGGTCTTGTTTGGAAAGAAATATGGTTCTGGCGGCGGCTAA
Protein sequence

>MS.gene065947.t1

MADGPHHPSIAQKIGGHSYLVSRLSANSNYRNYSTSGTFTNGVQTSGLALASPLSSFTVPSPAEKGPAGFLIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMLKSGRLSEPYKGIGDCFARTMKDEGIIALWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDRDGYWKWFAGNLVSGGAAGASSLLFVYSLDYARTRLANDAKAAKKGGERQFNGMVDVYKKTLQSDGIAGLYRGFNISCVGIIVYRGLYFGMYDSLKPVVLVGGMQDSFFASFLLGWGITIGAGLASYPIDTVRRRMMMTSGEAVKYNSSLHAFQTIVAKEGTKSLFKGAGANILRAVAGAGVLAGYDKLQLVLFGKKYGSGGG