AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60471

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60471.t1 MTR_3g028590 98.972 389 4 0 1 389 5 393 0.0 790
MS.gene60471.t1 MTR_3g028590 98.972 389 4 0 1 389 1 389 0.0 789
MS.gene60471.t1 MTR_8g036880 81.795 390 45 4 1 389 35 399 0.0 609
MS.gene60471.t1 MTR_4g078545 75.128 390 75 3 1 388 1 370 0.0 546
MS.gene60471.t1 MTR_2g098070 74.444 360 83 4 35 389 7 362 7.44e-178 499
MS.gene60471.t1 MTR_8g107270 59.649 285 115 0 92 376 17 301 3.58e-128 371
MS.gene60471.t1 MTR_3g104500 39.527 296 168 6 87 376 28 318 1.59e-67 217
MS.gene60471.t1 MTR_3g104600 31.715 309 177 9 89 376 31 326 1.76e-35 133
MS.gene60471.t1 MTR_6g091800 28.713 303 187 7 89 376 15 303 1.21e-32 125
MS.gene60471.t1 MTR_6g091800 27.245 323 190 7 89 376 15 327 9.45e-29 115
MS.gene60471.t1 MTR_6g091800 28.839 267 164 6 89 344 15 266 1.82e-28 112
MS.gene60471.t1 MTR_5g009500 29.022 317 172 10 81 363 24 321 3.70e-28 113
MS.gene60471.t1 MTR_6g091800 30.204 245 154 4 89 326 15 249 1.36e-27 110
MS.gene60471.t1 MTR_8g027975 29.195 298 174 9 92 363 50 336 2.78e-27 111
MS.gene60471.t1 MTR_8g027975 26.108 203 131 5 96 285 157 353 7.76e-11 63.5
MS.gene60471.t1 MTR_6g091800 27.273 308 180 6 89 362 15 312 3.58e-27 110
MS.gene60471.t1 MTR_7g064980 28.904 301 195 8 90 379 22 314 8.38e-27 109
MS.gene60471.t1 MTR_1g012030 30.314 287 162 11 93 368 139 398 2.14e-26 109
MS.gene60471.t1 MTR_1g012030 27.143 210 132 6 81 286 219 411 3.29e-14 73.9
MS.gene60471.t1 MTR_3g107510 28.881 277 173 8 96 368 125 381 7.92e-26 107
MS.gene60471.t1 MTR_3g107510 28.095 210 130 6 81 286 202 394 1.45e-15 78.2
MS.gene60471.t1 MTR_8g096050 29.617 287 168 9 93 368 227 490 2.29e-25 107
MS.gene60471.t1 MTR_7g084400 30.345 290 159 8 93 368 213 473 5.44e-25 106
MS.gene60471.t1 MTR_7g068340 29.329 283 169 8 104 379 98 356 1.03e-24 104
MS.gene60471.t1 MTR_6g091800 27.148 291 162 7 89 344 15 290 1.20e-24 102
MS.gene60471.t1 MTR_4g119770 26.444 329 195 8 83 375 4 321 2.17e-24 102
MS.gene60471.t1 MTR_6g091800 28.253 269 152 5 89 326 15 273 7.58e-24 100
MS.gene60471.t1 MTR_4g114320 27.465 284 174 7 93 368 123 382 9.01e-24 102
MS.gene60471.t1 MTR_4g114320 27.751 209 127 6 83 286 206 395 2.55e-14 74.3
MS.gene60471.t1 MTR_5g081150 26.144 306 209 7 83 379 24 321 1.39e-23 100
MS.gene60471.t1 MTR_3g098460 28.053 303 181 11 77 368 192 468 3.90e-23 101
MS.gene60471.t1 MTR_2g436930 27.358 318 186 9 90 373 12 318 9.85e-23 98.2
MS.gene60471.t1 MTR_6g034145 28.340 247 163 5 141 376 6 249 1.28e-22 96.3
MS.gene60471.t1 MTR_6g091800 31.148 183 110 3 89 264 15 188 1.49e-22 94.4
MS.gene60471.t1 MTR_4g016730 29.191 346 203 13 40 379 46 355 1.95e-22 98.2
MS.gene60471.t1 MTR_6g092210 28.216 241 165 5 141 376 6 243 4.38e-22 94.7
MS.gene60471.t1 MTR_4g119770 26.592 267 160 6 83 324 4 259 2.20e-19 87.4
MS.gene60471.t1 MTR_6g091800 28.502 207 108 4 89 264 15 212 1.25e-18 84.0
MS.gene60471.t1 MTR_3g074490 27.946 297 166 12 96 368 119 391 1.70e-17 84.0
MS.gene60471.t1 MTR_4g005330 24.000 300 186 7 90 368 63 341 3.00e-17 82.8
MS.gene60471.t1 MTR_1g090330 22.180 266 195 4 93 354 14 271 5.22e-17 81.3
MS.gene60471.t1 MTR_1g090330 28.571 168 112 4 94 260 112 272 7.09e-13 68.9
MS.gene60471.t1 MTR_2g102010 25.874 286 195 6 92 373 16 288 7.77e-17 80.9
MS.gene60471.t1 MTR_4g005330 23.810 294 182 7 96 368 3 275 3.65e-16 78.6
MS.gene60471.t1 MTR_4g012850 26.749 243 164 5 138 373 51 286 8.09e-16 77.8
MS.gene60471.t1 MTR_3g100570 25.839 298 180 10 93 361 6 291 8.50e-16 77.8
MS.gene60471.t1 MTR_5g030530 26.502 283 187 9 92 363 7 279 9.21e-16 77.4
MS.gene60471.t1 MTR_6g091910 26.866 201 136 5 89 285 15 208 1.93e-15 75.1
MS.gene60471.t1 MTR_8g066975 26.316 285 185 9 92 363 7 279 2.04e-15 76.6
MS.gene60471.t1 MTR_7g084400 31.122 196 109 5 93 282 207 382 4.02e-15 77.0
MS.gene60471.t1 MTR_7g066690 25.000 272 187 7 89 354 13 273 2.92e-14 73.2
MS.gene60471.t1 MTR_5g081150 28.966 145 97 2 83 223 24 166 9.89e-14 70.9
MS.gene60471.t1 MTR_5g081150 28.966 145 97 2 83 223 24 166 1.27e-13 70.9
MS.gene60471.t1 MTR_4g018750 25.461 271 181 9 99 361 21 278 1.69e-13 70.9
MS.gene60471.t1 MTR_2g436930 29.231 195 111 5 200 373 40 228 6.81e-13 68.2
MS.gene60471.t1 MTR_8g090210 23.649 296 180 10 96 368 113 385 1.62e-12 68.9
MS.gene60471.t1 MTR_4g018750 25.000 256 171 9 99 346 21 263 1.80e-11 64.7
MS.gene60471.t1 MTR_5g032420 21.405 299 201 6 93 361 6 300 4.00e-11 63.9
MS.gene60471.t1 MTR_1g088995 24.444 270 177 7 92 354 32 281 7.30e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60471.t1 AT5G13490 80.916 393 63 5 1 389 1 385 0.0 608
MS.gene60471.t1 AT5G13490 80.916 393 63 5 1 389 1 385 0.0 608
MS.gene60471.t1 AT3G08580 82.188 393 54 6 1 389 1 381 0.0 590
MS.gene60471.t1 AT3G08580 82.188 393 54 6 1 389 1 381 0.0 590
MS.gene60471.t1 AT4G28390 74.936 391 81 4 2 388 1 378 0.0 548
MS.gene60471.t1 AT4G28390 74.936 391 81 4 2 388 1 378 0.0 548
MS.gene60471.t1 AT5G17400 59.136 301 123 0 80 380 1 301 2.99e-130 376
MS.gene60471.t1 AT5G56450 39.159 309 169 7 80 376 21 322 3.04e-66 213
MS.gene60471.t1 AT3G55640 28.037 321 213 7 58 368 1 313 3.23e-33 127
MS.gene60471.t1 AT3G55640 28.037 321 213 7 58 368 1 313 3.23e-33 127
MS.gene60471.t1 AT1G14560 30.249 281 170 6 104 368 38 308 7.19e-33 126
MS.gene60471.t1 AT2G37890 30.640 297 189 7 92 379 44 332 8.87e-33 125
MS.gene60471.t1 AT1G14560 30.545 275 165 6 104 362 38 302 5.21e-32 123
MS.gene60471.t1 AT4G26180 31.270 307 177 9 89 377 17 307 1.22e-31 122
MS.gene60471.t1 AT4G26180 31.959 291 165 8 89 362 17 291 1.34e-30 119
MS.gene60471.t1 AT3G53940 29.831 295 187 8 85 368 63 348 3.81e-29 116
MS.gene60471.t1 AT3G53940 23.858 197 138 3 91 287 178 362 1.89e-11 65.5
MS.gene60471.t1 AT3G51870 32.979 282 160 9 104 379 102 360 9.23e-29 115
MS.gene60471.t1 AT4G01100 27.597 308 168 9 96 368 45 332 1.75e-26 108
MS.gene60471.t1 AT5G01500 30.466 279 171 9 104 379 130 388 3.17e-26 109
MS.gene60471.t1 AT4G32400 29.893 281 171 9 93 368 114 373 1.43e-25 107
MS.gene60471.t1 AT4G01100 27.796 313 175 9 96 368 45 346 1.00e-24 104
MS.gene60471.t1 AT4G01100 26.821 302 166 9 102 368 1 282 2.91e-24 102
MS.gene60471.t1 AT5G51050 30.175 285 166 7 93 368 223 483 7.50e-24 103
MS.gene60471.t1 AT3G21390 26.625 323 198 7 83 373 9 324 3.26e-23 99.8
MS.gene60471.t1 AT3G21390 26.625 323 198 7 83 373 31 346 4.18e-23 99.8
MS.gene60471.t1 AT5G61810 27.703 296 183 8 77 368 52 320 4.78e-23 99.4
MS.gene60471.t1 AT5G61810 27.703 296 183 8 77 368 195 463 3.28e-22 98.6
MS.gene60471.t1 AT3G55640 27.481 262 171 6 58 312 1 250 4.32e-22 94.7
MS.gene60471.t1 AT3G55640 27.481 262 171 6 58 312 1 250 4.32e-22 94.7
MS.gene60471.t1 AT1G14560 31.383 188 112 4 197 368 24 210 6.99e-22 93.6
MS.gene60471.t1 AT3G20240 24.917 301 190 6 86 368 45 327 9.36e-22 95.9
MS.gene60471.t1 AT5G48970 27.160 324 196 10 83 373 12 328 1.93e-21 94.7
MS.gene60471.t1 AT5G07320 27.778 270 169 7 102 368 218 464 7.70e-21 94.4
MS.gene60471.t1 AT3G53940 29.167 216 137 5 85 293 63 269 1.07e-18 85.9
MS.gene60471.t1 AT5G64970 27.119 295 171 11 96 368 138 410 6.30e-15 76.3
MS.gene60471.t1 AT5G48970 26.641 259 157 8 141 373 6 257 7.16e-15 74.7
MS.gene60471.t1 AT1G14140 23.881 268 189 6 93 354 16 274 8.15e-15 75.1
MS.gene60471.t1 AT1G14140 28.061 196 127 5 198 383 18 209 1.08e-11 65.9
MS.gene60471.t1 AT5G64970 26.441 295 173 11 96 368 14 286 2.78e-14 73.6
MS.gene60471.t1 AT5G64970 27.431 288 165 11 96 361 138 403 5.14e-14 73.6
MS.gene60471.t1 AT5G46800 26.573 286 186 8 92 363 7 282 5.77e-14 72.4
MS.gene60471.t1 AT5G46800 26.573 286 186 8 92 363 7 282 5.77e-14 72.4
MS.gene60471.t1 AT5G46800 26.573 286 186 8 92 363 7 282 5.77e-14 72.4
MS.gene60471.t1 AT5G58970 24.399 291 191 8 83 361 6 279 1.29e-13 71.2
MS.gene60471.t1 AT2G33820 23.759 282 200 5 89 362 14 288 2.49e-13 70.5
MS.gene60471.t1 AT5G66380 27.317 205 125 6 191 383 5 197 1.27e-12 68.6
MS.gene60471.t1 AT3G54110 23.134 268 189 8 93 354 15 271 1.92e-12 67.8
MS.gene60471.t1 AT1G79900 25.726 241 156 6 140 373 53 277 7.99e-12 65.9
MS.gene60471.t1 AT1G78180 24.415 299 174 8 96 368 131 403 1.33e-11 66.2
MS.gene60471.t1 AT2G33820 23.103 290 200 6 89 362 14 296 5.45e-11 63.5

Find 123 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTGGTGCTGGTGTGCTTTC+TGG 0.149238 3.2:+19984516 MS.gene60471:CDS
TGATCATCTCATCCTGGTTT+TGG 0.179358 3.2:-19983549 None:intergenic
TGGTCTTTCATCTGGATTTA+GGG 0.236214 3.2:+19983264 MS.gene60471:CDS
CTGGTCTTTCATCTGGATTT+AGG 0.248794 3.2:+19983263 MS.gene60471:CDS
AATGGGAGCAGCAGCAGTTT+TGG 0.270825 3.2:-19983506 None:intergenic
AACATCCTCCGTGCCGTTGC+TGG 0.301686 3.2:+19984498 MS.gene60471:CDS
AGTTCCCATAGGAGAATCTT+TGG 0.306546 3.2:-19983321 None:intergenic
TCTTCTCACTGGAAAATTGC+AGG 0.310516 3.2:+19984140 MS.gene60471:CDS
CTTAGCTGCCTTAGAATCAT+TGG 0.323339 3.2:-19983956 None:intergenic
CTTGCTACTGATGGAATTGC+TGG 0.330650 3.2:+19984030 MS.gene60471:CDS
AGAAGGACAGGGATGGTTAC+TGG 0.330831 3.2:+19983839 MS.gene60471:CDS
CCTCATTCTTGAGGATTTGT+TGG 0.335787 3.2:-19984443 None:intergenic
CTTCTCCTAACAGTGTCAAT+AGG 0.342407 3.2:-19984366 None:intergenic
CTCCGTGCCGTTGCTGGTGC+TGG 0.343034 3.2:+19984504 MS.gene60471:CDS
CGTAGATCTGGTCTTTCATC+TGG 0.358197 3.2:+19983256 MS.gene60471:CDS
ACAATCACCAATACCCTTGT+AGG 0.369255 3.2:-19983593 None:intergenic
ATGAGGGTGTTGTTGCTCTC+TGG 0.371630 3.2:+19983635 MS.gene60471:CDS
ACCTCCAGATGCCAAGTTAC+CGG 0.389000 3.2:-19983872 None:intergenic
GTGACCTTCTCCATGATTGT+TGG 0.389407 3.2:-19983220 None:intergenic
CAAAAGCTTAATACGTTCAA+TGG 0.391915 3.2:-19983524 None:intergenic
TCGTCTTGCCAATGATTCTA+AGG 0.397580 3.2:+19983948 MS.gene60471:CDS
ACAGGGATGGTTACTGGAAA+TGG 0.399761 3.2:+19983845 MS.gene60471:CDS
TTCTGGTTACGACAAGTTGC+AGG 0.414142 3.2:+19984533 MS.gene60471:CDS
GAAAGCAACCATGGTTGATC+AGG 0.420030 3.2:+19983189 None:intergenic
TTGGCATCTGGAGGTGCTGC+TGG 0.420414 3.2:+19983880 MS.gene60471:CDS
TTTCGTTATTGACTTTCTCA+TGG 0.438686 3.2:+19983462 MS.gene60471:CDS
TTCTCCTAACAGTGTCAATA+GGG 0.439922 3.2:-19984365 None:intergenic
CTTGTGTTCGGCAAGAAATA+CGG 0.440747 3.2:+19984558 MS.gene60471:CDS
AAGTTGCAGGTTCTTGTGTT+CGG 0.444616 3.2:+19984546 MS.gene60471:CDS
TTTGCCGGTAACTTGGCATC+TGG 0.445959 3.2:+19983868 MS.gene60471:CDS
AGTGAGAAGAACTGGTTTGA+GGG 0.449368 3.2:-19984127 None:intergenic
GGCCAGCTCCACCGTAGATC+TGG 0.449489 3.2:+19983244 MS.gene60471:CDS
GAGCCATCCAAGCGCAAAGC+TGG 0.450450 3.2:-19984313 None:intergenic
CAGTGAGAAGAACTGGTTTG+AGG 0.452118 3.2:-19984128 None:intergenic
GGTTACTGGAAATGGTTTGC+CGG 0.452634 3.2:+19983853 MS.gene60471:CDS
CTTGTACTTAACAGCTTCTC+CGG 0.452946 3.2:-19984403 None:intergenic
GAAATGGTTTGCCGGTAACT+TGG 0.453917 3.2:+19983861 MS.gene60471:CDS
TGAGAAAGTCAATAACGAAA+TGG 0.456385 3.2:-19983459 None:intergenic
TCGCCAGCTTTGCGCTTGGA+TGG 0.458299 3.2:+19984310 MS.gene60471:CDS
CTCAAACCAGTTCTTCTCAC+TGG 0.459621 3.2:+19984129 MS.gene60471:CDS
CATACCCTATTGACACTGTT+AGG 0.463766 3.2:+19984361 MS.gene60471:CDS
GACCAGATCTACGGTGGAGC+TGG 0.465874 3.2:-19983246 None:intergenic
GCTGGAGCAGCGACGAAGAC+GGG 0.467128 3.2:-19983427 None:intergenic
CACCAGCACCAGCAACGGCA+CGG 0.468876 3.2:-19984506 None:intergenic
GTTCCCATAGGAGAATCTTT+GGG 0.471265 3.2:-19983320 None:intergenic
TCTGGATTTAGGGGTTATGA+AGG 0.477435 3.2:+19983274 MS.gene60471:CDS
ATCATTGGCAAGACGAGTAC+GGG 0.477887 3.2:-19983941 None:intergenic
ATCAACAGTTGCCCTGCAAG+TGG 0.483410 3.2:-19983377 None:intergenic
TCTGAGCCCTACAAGGGTAT+TGG 0.488103 3.2:+19983586 MS.gene60471:CDS
AAAAGCTTAATACGTTCAAT+GGG 0.489695 3.2:-19983523 None:intergenic
ATGTCCCAAAGATTCTCCTA+TGG 0.492338 3.2:+19983316 MS.gene60471:CDS
GGTTTCAACATCTCCTGTGT+TGG 0.492793 3.2:+19984063 MS.gene60471:CDS
TTCGTTATTGACTTTCTCAT+GGG 0.493555 3.2:+19983463 MS.gene60471:CDS
CCAACAAATCCTCAAGAATG+AGG 0.494163 3.2:+19984443 MS.gene60471:CDS
CAGGATGAGATGATCAAGTC+TGG 0.496357 3.2:+19983556 MS.gene60471:CDS
AAGCACACCAGCACCAGCAA+CGG 0.501311 3.2:-19984511 None:intergenic
GGGTGCTAAGTCATTGTTCA+AGG 0.502290 3.2:+19984464 MS.gene60471:CDS
AATCATTGGCAAGACGAGTA+CGG 0.503375 3.2:-19983942 None:intergenic
GTCTACCGTGGTCTGTACTT+CGG 0.505449 3.2:+19984093 MS.gene60471:CDS
AAGAAGACTCTTGCTACTGA+TGG 0.508472 3.2:+19984021 MS.gene60471:CDS
TAAAAGAACAATGGCTGATG+AGG 0.509418 3.2:+19983618 MS.gene60471:CDS
TAACCTGAGTGGGGAAATAA+CGG 0.511945 3.2:-19983681 None:intergenic
ACAATCATGGAGAAGGTCAC+TGG 0.514525 3.2:+19983223 MS.gene60471:CDS
AGAAAACAACTAACCTGAGT+GGG 0.518937 3.2:-19983691 None:intergenic
TTCGGCAAGAAATACGGATC+TGG 0.519522 3.2:+19984564 MS.gene60471:CDS
CAGACTTGATCATCTCATCC+TGG 0.520517 3.2:-19983555 None:intergenic
AAGTCATTGTTCAAGGGAGC+TGG 0.521554 3.2:+19984471 MS.gene60471:CDS
GGTTAAGCAACCAACAATCA+TGG 0.524656 3.2:+19983210 MS.gene60471:CDS
TGATGCTAGACCTGCACCAT+TGG 0.527310 3.2:-19984340 None:intergenic
AGAAGAATGATGATGACATC+CGG 0.527583 3.2:+19984384 MS.gene60471:CDS
CAACAGTTGCCCTGCAAGTG+GGG 0.528651 3.2:-19983375 None:intergenic
TGGCTCATTACCAATGGTGC+AGG 0.529441 3.2:+19984330 MS.gene60471:CDS
TAGGCTCTCTGAGCCCTACA+AGG 0.529504 3.2:+19983579 MS.gene60471:CDS
GGTCTTTCATCTGGATTTAG+GGG 0.530879 3.2:+19983265 MS.gene60471:CDS
CATTCAAAGATTACTTCAAG+AGG 0.536246 3.2:+19983803 MS.gene60471:CDS
GATTCTAAGGCAGCTAAGAA+AGG 0.539062 3.2:+19983961 MS.gene60471:CDS
CAGCTAAGAAAGGTGGAGAA+AGG 0.539801 3.2:+19983971 MS.gene60471:CDS
GCCGGTAACTTGGCATCTGG+AGG 0.547072 3.2:+19983871 MS.gene60471:CDS
GAGAATCTTTGGGACATGGC+AGG 0.547914 3.2:-19983310 None:intergenic
GTTATTGACTTTCTCATGGG+TGG 0.552168 3.2:+19983466 MS.gene60471:CDS
TTGCTACTGATGGAATTGCT+GGG 0.552525 3.2:+19984031 MS.gene60471:CDS
ACTTAGCACCCTCATTCTTG+AGG 0.554699 3.2:-19984452 None:intergenic
TCAACAGTTGCCCTGCAAGT+GGG 0.556580 3.2:-19983376 None:intergenic
AAAAGAACAATGGCTGATGA+GGG 0.560769 3.2:+19983619 MS.gene60471:CDS
GGACATGGCAGGATGGCGGT+AGG 0.563819 3.2:-19983299 None:intergenic
TGTCCCAAAGATTCTCCTAT+GGG 0.564873 3.2:+19983317 MS.gene60471:CDS
GATTTGTTGGAATGCATCAA+AGG 0.565548 3.2:-19984430 None:intergenic
TATCCGTTATTTCCCCACTC+AGG 0.571053 3.2:+19983678 MS.gene60471:CDS
GGTGGAGAAAGGCAGTTCAA+TGG 0.572074 3.2:+19983982 MS.gene60471:CDS
TAGAAAACAACTAACCTGAG+TGG 0.579173 3.2:-19983692 None:intergenic
CTTGGATGGCTCATTACCAA+TGG 0.580145 3.2:+19984324 MS.gene60471:CDS
CAATCACCAATACCCTTGTA+GGG 0.581476 3.2:-19983592 None:intergenic
TGCTGGAGCAGCGACGAAGA+CGG 0.584125 3.2:-19983428 None:intergenic
AACAGTTGCCCTGCAAGTGG+GGG 0.584275 3.2:-19983374 None:intergenic
CAGATGAAAGACCAGATCTA+CGG 0.593332 3.2:-19983255 None:intergenic
ATGAGATGATCAAGTCTGGT+AGG 0.599652 3.2:+19983560 MS.gene60471:CDS
CAAGAGAGTAAACAAACAAG+AGG 0.602030 3.2:-19983912 None:intergenic
ATCTTTGGGACATGGCAGGA+TGG 0.604045 3.2:-19983306 None:intergenic
GGTGTTGTTGCTCTCTGGAG+AGG 0.606867 3.2:+19983640 MS.gene60471:CDS
AACTTCAAGAAGGACAGGGA+TGG 0.606997 3.2:+19983832 MS.gene60471:CDS
CGCTGCATTGCAGAATCCAA+TGG 0.607456 3.2:+19983351 MS.gene60471:CDS
CAACAAATCCTCAAGAATGA+GGG 0.607876 3.2:+19984444 MS.gene60471:CDS
GCAACCAACAATCATGGAGA+AGG 0.628211 3.2:+19983216 MS.gene60471:CDS
GGCAAGAAATACGGATCTGG+TGG 0.628657 3.2:+19984567 MS.gene60471:CDS
TCTAAGGCAGCTAAGAAAGG+TGG 0.629619 3.2:+19983964 MS.gene60471:CDS
GTAAACAAACAAGAGGGATG+AGG 0.631365 3.2:-19983905 None:intergenic
CACAGGAGATGTTGAAACCA+CGG 0.633206 3.2:-19984059 None:intergenic
ATAGGAGAATCTTTGGGACA+TGG 0.635000 3.2:-19983314 None:intergenic
TTTCAACTTCAAGAAGGACA+GGG 0.637221 3.2:+19983828 MS.gene60471:CDS
GGTGCTAAGTCATTGTTCAA+GGG 0.642143 3.2:+19984465 MS.gene60471:CDS
GGAATTGCTGGGCTTTACCG+TGG 0.654046 3.2:+19984042 MS.gene60471:CDS
AAGAGAGTAAACAAACAAGA+GGG 0.655445 3.2:-19983911 None:intergenic
GTAGACAATGATTCCAACAC+AGG 0.657927 3.2:-19984076 None:intergenic
GGGGAAATAACGGATAACAT+TGG 0.658006 3.2:-19983671 None:intergenic
AGCGTTTGAGTAGTTCCCAT+AGG 0.663946 3.2:-19983332 None:intergenic
CAGCACCAGCAACGGCACGG+AGG 0.668727 3.2:-19984503 None:intergenic
GAAAACAACTAACCTGAGTG+GGG 0.671762 3.2:-19983690 None:intergenic
TTGCTTAACCTGATCAACCA+TGG 0.675233 3.2:-19983197 None:intergenic
ATGAAAGACCAGATCTACGG+TGG 0.675575 3.2:-19983252 None:intergenic
AGGCTCTCTGAGCCCTACAA+GGG 0.676799 3.2:+19983580 MS.gene60471:CDS
TTTGGGACATGGCAGGATGG+CGG 0.679753 3.2:-19983303 None:intergenic
GTTGGAATCATTGTCTACCG+TGG 0.700486 3.2:+19984081 MS.gene60471:CDS
ACAATCCGAAGTACAGACCA+CGG 0.718426 3.2:-19984098 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGCTATTTAAATTGTTTT+AGG + chr3.2:19983768-19983787 MS.gene60471:intron 15.0%
!! AATGAATATCATTTGTTCAA+AGG - chr3.2:19984179-19984198 None:intergenic 20.0%
!! ATGAATATCATTTGTTCAAA+GGG - chr3.2:19984178-19984197 None:intergenic 20.0%
!! CATTAAATACACAAAACAAT+TGG - chr3.2:19983738-19983757 None:intergenic 20.0%
!! TGCATCGAAAAATAATAATT+TGG - chr3.2:19984206-19984225 None:intergenic 20.0%
!!! TGATTGTTTTAAAAGAACAA+TGG + chr3.2:19983609-19983628 MS.gene60471:CDS 20.0%
! AAAAGCTTAATACGTTCAAT+GGG - chr3.2:19983526-19983545 None:intergenic 25.0%
AAGAGAGTAAACAAACAAGA+GGG - chr3.2:19983914-19983933 None:intergenic 30.0%
AATACACAAAACAATTGGAC+AGG - chr3.2:19983733-19983752 None:intergenic 30.0%
CAAAAGCTTAATACGTTCAA+TGG - chr3.2:19983527-19983546 None:intergenic 30.0%
CATTCAAAGATTACTTCAAG+AGG + chr3.2:19983803-19983822 MS.gene60471:CDS 30.0%
TGAGAAAGTCAATAACGAAA+TGG - chr3.2:19983462-19983481 None:intergenic 30.0%
! TTTTGAACTCTTGACATTAC+AGG + chr3.2:19984278-19984297 MS.gene60471:intron 30.0%
!! TTCGTTATTGACTTTCTCAT+GGG + chr3.2:19983463-19983482 MS.gene60471:CDS 30.0%
!! TTTCGTTATTGACTTTCTCA+TGG + chr3.2:19983462-19983481 MS.gene60471:CDS 30.0%
AAAAGAACAATGGCTGATGA+GGG + chr3.2:19983619-19983638 MS.gene60471:CDS 35.0%
AGAAAACAACTAACCTGAGT+GGG - chr3.2:19983694-19983713 None:intergenic 35.0%
CAACAAATCCTCAAGAATGA+GGG + chr3.2:19984444-19984463 MS.gene60471:CDS 35.0%
CAAGAGAGTAAACAAACAAG+AGG - chr3.2:19983915-19983934 None:intergenic 35.0%
TAAAAGAACAATGGCTGATG+AGG + chr3.2:19983618-19983637 MS.gene60471:CDS 35.0%
TAGAAAACAACTAACCTGAG+TGG - chr3.2:19983695-19983714 None:intergenic 35.0%
TGGTCTTTCATCTGGATTTA+GGG + chr3.2:19983264-19983283 MS.gene60471:CDS 35.0%
TTCTCCTAACAGTGTCAATA+GGG - chr3.2:19984368-19984387 None:intergenic 35.0%
TTTCAACTTCAAGAAGGACA+GGG + chr3.2:19983828-19983847 MS.gene60471:CDS 35.0%
! AATTTTCCAGTGAGAAGAAC+TGG - chr3.2:19984138-19984157 None:intergenic 35.0%
! AGAAGAATGATGATGACATC+CGG + chr3.2:19984384-19984403 MS.gene60471:CDS 35.0%
! TAAGCTTTTGATCCAAAACC+AGG + chr3.2:19983537-19983556 MS.gene60471:CDS 35.0%
! TTTTCAACTTCAAGAAGGAC+AGG + chr3.2:19983827-19983846 MS.gene60471:CDS 35.0%
!! GATTTGTTGGAATGCATCAA+AGG - chr3.2:19984433-19984452 None:intergenic 35.0%
AAGAAGACTCTTGCTACTGA+TGG + chr3.2:19984021-19984040 MS.gene60471:CDS 40.0%
AAGTTGCAGGTTCTTGTGTT+CGG + chr3.2:19984546-19984565 MS.gene60471:CDS 40.0%
AATCATTGGCAAGACGAGTA+CGG - chr3.2:19983945-19983964 None:intergenic 40.0%
ACAATCACCAATACCCTTGT+AGG - chr3.2:19983596-19983615 None:intergenic 40.0%
AGTTCCCATAGGAGAATCTT+TGG - chr3.2:19983324-19983343 None:intergenic 40.0%
ATGAGATGATCAAGTCTGGT+AGG + chr3.2:19983560-19983579 MS.gene60471:CDS 40.0%
ATGTCCCAAAGATTCTCCTA+TGG + chr3.2:19983316-19983335 MS.gene60471:CDS 40.0%
CAATCACCAATACCCTTGTA+GGG - chr3.2:19983595-19983614 None:intergenic 40.0%
CAGATGAAAGACCAGATCTA+CGG - chr3.2:19983258-19983277 None:intergenic 40.0%
CATACCCTATTGACACTGTT+AGG + chr3.2:19984361-19984380 MS.gene60471:CDS 40.0%
CCAACAAATCCTCAAGAATG+AGG + chr3.2:19984443-19984462 MS.gene60471:CDS 40.0%
CCTCATTCTTGAGGATTTGT+TGG - chr3.2:19984446-19984465 None:intergenic 40.0%
CTTAGCTGCCTTAGAATCAT+TGG - chr3.2:19983959-19983978 None:intergenic 40.0%
CTTCTCCTAACAGTGTCAAT+AGG - chr3.2:19984369-19984388 None:intergenic 40.0%
CTTGTACTTAACAGCTTCTC+CGG - chr3.2:19984406-19984425 None:intergenic 40.0%
CTTGTGTTCGGCAAGAAATA+CGG + chr3.2:19984558-19984577 MS.gene60471:CDS 40.0%
GAAAACAACTAACCTGAGTG+GGG - chr3.2:19983693-19983712 None:intergenic 40.0%
GGTCTTTCATCTGGATTTAG+GGG + chr3.2:19983265-19983284 MS.gene60471:CDS 40.0%
GGTGCTAAGTCATTGTTCAA+GGG + chr3.2:19984465-19984484 MS.gene60471:CDS 40.0%
GGTTAAGCAACCAACAATCA+TGG + chr3.2:19983210-19983229 MS.gene60471:CDS 40.0%
GTAAACAAACAAGAGGGATG+AGG - chr3.2:19983908-19983927 None:intergenic 40.0%
GTAGACAATGATTCCAACAC+AGG - chr3.2:19984079-19984098 None:intergenic 40.0%
GTTCCCATAGGAGAATCTTT+GGG - chr3.2:19983323-19983342 None:intergenic 40.0%
TAACCTGAGTGGGGAAATAA+CGG - chr3.2:19983684-19983703 None:intergenic 40.0%
TCGTCTTGCCAATGATTCTA+AGG + chr3.2:19983948-19983967 MS.gene60471:CDS 40.0%
TCTGGATTTAGGGGTTATGA+AGG + chr3.2:19983274-19983293 MS.gene60471:CDS 40.0%
TCTTCTCACTGGAAAATTGC+AGG + chr3.2:19984140-19984159 MS.gene60471:CDS 40.0%
TGTCCCAAAGATTCTCCTAT+GGG + chr3.2:19983317-19983336 MS.gene60471:CDS 40.0%
TTGCTACTGATGGAATTGCT+GGG + chr3.2:19984031-19984050 MS.gene60471:CDS 40.0%
! CTGGTCTTTCATCTGGATTT+AGG + chr3.2:19983263-19983282 MS.gene60471:CDS 40.0%
! GAGGCTTTTCAACTTCAAGA+AGG + chr3.2:19983822-19983841 MS.gene60471:CDS 40.0%
! TGATCATCTCATCCTGGTTT+TGG - chr3.2:19983552-19983571 None:intergenic 40.0%
!! AGTGAGAAGAACTGGTTTGA+GGG - chr3.2:19984130-19984149 None:intergenic 40.0%
!! ATAGGAGAATCTTTGGGACA+TGG - chr3.2:19983317-19983336 None:intergenic 40.0%
!! GATTCTAAGGCAGCTAAGAA+AGG + chr3.2:19983961-19983980 MS.gene60471:CDS 40.0%
!! GGGGAAATAACGGATAACAT+TGG - chr3.2:19983674-19983693 None:intergenic 40.0%
!! GTTATTGACTTTCTCATGGG+TGG + chr3.2:19983466-19983485 MS.gene60471:CDS 40.0%
AACTTCAAGAAGGACAGGGA+TGG + chr3.2:19983832-19983851 MS.gene60471:CDS 45.0%
AAGTCATTGTTCAAGGGAGC+TGG + chr3.2:19984471-19984490 MS.gene60471:CDS 45.0%
ACAATCATGGAGAAGGTCAC+TGG + chr3.2:19983223-19983242 MS.gene60471:CDS 45.0%
ACAATCCGAAGTACAGACCA+CGG - chr3.2:19984101-19984120 None:intergenic 45.0%
ACAGGGATGGTTACTGGAAA+TGG + chr3.2:19983845-19983864 MS.gene60471:CDS 45.0%
ACTTAGCACCCTCATTCTTG+AGG - chr3.2:19984455-19984474 None:intergenic 45.0%
AGCGTTTGAGTAGTTCCCAT+AGG - chr3.2:19983335-19983354 None:intergenic 45.0%
ATCATTGGCAAGACGAGTAC+GGG - chr3.2:19983944-19983963 None:intergenic 45.0%
ATGAAAGACCAGATCTACGG+TGG - chr3.2:19983255-19983274 None:intergenic 45.0%
CACAGGAGATGTTGAAACCA+CGG - chr3.2:19984062-19984081 None:intergenic 45.0%
CAGACTTGATCATCTCATCC+TGG - chr3.2:19983558-19983577 None:intergenic 45.0%
CAGCTAAGAAAGGTGGAGAA+AGG + chr3.2:19983971-19983990 MS.gene60471:CDS 45.0%
CAGGATGAGATGATCAAGTC+TGG + chr3.2:19983556-19983575 MS.gene60471:CDS 45.0%
CTCAAACCAGTTCTTCTCAC+TGG + chr3.2:19984129-19984148 MS.gene60471:CDS 45.0%
CTTGCTACTGATGGAATTGC+TGG + chr3.2:19984030-19984049 MS.gene60471:CDS 45.0%
CTTGGATGGCTCATTACCAA+TGG + chr3.2:19984324-19984343 MS.gene60471:CDS 45.0%
GCAACCAACAATCATGGAGA+AGG + chr3.2:19983216-19983235 MS.gene60471:CDS 45.0%
GGGTGCTAAGTCATTGTTCA+AGG + chr3.2:19984464-19984483 MS.gene60471:CDS 45.0%
GGTTTCAACATCTCCTGTGT+TGG + chr3.2:19984063-19984082 MS.gene60471:CDS 45.0%
GTGACCTTCTCCATGATTGT+TGG - chr3.2:19983223-19983242 None:intergenic 45.0%
GTTGGAATCATTGTCTACCG+TGG + chr3.2:19984081-19984100 MS.gene60471:CDS 45.0%
TTCGGCAAGAAATACGGATC+TGG + chr3.2:19984564-19984583 MS.gene60471:CDS 45.0%
! ACGAAATGGCTCTTTTCTGC+TGG - chr3.2:19983448-19983467 None:intergenic 45.0%
! CGTAGATCTGGTCTTTCATC+TGG + chr3.2:19983256-19983275 MS.gene60471:CDS 45.0%
! GAAATGGTTTGCCGGTAACT+TGG + chr3.2:19983861-19983880 MS.gene60471:CDS 45.0%
! GGTTACTGGAAATGGTTTGC+CGG + chr3.2:19983853-19983872 MS.gene60471:CDS 45.0%
! TATCCGTTATTTCCCCACTC+AGG + chr3.2:19983678-19983697 MS.gene60471:CDS 45.0%
! TCTAAGGCAGCTAAGAAAGG+TGG + chr3.2:19983964-19983983 MS.gene60471:CDS 45.0%
! TTCTGGTTACGACAAGTTGC+AGG + chr3.2:19984533-19984552 MS.gene60471:CDS 45.0%
!! CAGTGAGAAGAACTGGTTTG+AGG - chr3.2:19984131-19984150 None:intergenic 45.0%
!!! AGCAGCAGCAGTTTTGGAAA+CGG - chr3.2:19983503-19983522 None:intergenic 45.0%
ACCTCCAGATGCCAAGTTAC+CGG - chr3.2:19983875-19983894 None:intergenic 50.0%
AGAAGGACAGGGATGGTTAC+TGG + chr3.2:19983839-19983858 MS.gene60471:CDS 50.0%
ATCAACAGTTGCCCTGCAAG+TGG - chr3.2:19983380-19983399 None:intergenic 50.0%
CGCTGCATTGCAGAATCCAA+TGG + chr3.2:19983351-19983370 MS.gene60471:CDS 50.0%
GGCAAGAAATACGGATCTGG+TGG + chr3.2:19984567-19984586 MS.gene60471:CDS 50.0%
GGTGGAGAAAGGCAGTTCAA+TGG + chr3.2:19983982-19984001 MS.gene60471:CDS 50.0%
GTCTACCGTGGTCTGTACTT+CGG + chr3.2:19984093-19984112 MS.gene60471:CDS 50.0%
TCAACAGTTGCCCTGCAAGT+GGG - chr3.2:19983379-19983398 None:intergenic 50.0%
TGATGCTAGACCTGCACCAT+TGG - chr3.2:19984343-19984362 None:intergenic 50.0%
TGGCTCATTACCAATGGTGC+AGG + chr3.2:19984330-19984349 MS.gene60471:CDS 50.0%
TTTGCCGGTAACTTGGCATC+TGG + chr3.2:19983868-19983887 MS.gene60471:CDS 50.0%
! AATGGGAGCAGCAGCAGTTT+TGG - chr3.2:19983509-19983528 None:intergenic 50.0%
! TCTGAGCCCTACAAGGGTAT+TGG + chr3.2:19983586-19983605 MS.gene60471:CDS 50.0%
! TTTTTCGCCAGCTTTGCGCT+TGG + chr3.2:19984306-19984325 MS.gene60471:CDS 50.0%
!! ATCTTTGGGACATGGCAGGA+TGG - chr3.2:19983309-19983328 None:intergenic 50.0%
!! ATGAGGGTGTTGTTGCTCTC+TGG + chr3.2:19983635-19983654 MS.gene60471:CDS 50.0%
!! GAGAATCTTTGGGACATGGC+AGG - chr3.2:19983313-19983332 None:intergenic 50.0%
AACAGTTGCCCTGCAAGTGG+GGG - chr3.2:19983377-19983396 None:intergenic 55.0%
AAGCACACCAGCACCAGCAA+CGG - chr3.2:19984514-19984533 None:intergenic 55.0%
AGGCTCTCTGAGCCCTACAA+GGG + chr3.2:19983580-19983599 MS.gene60471:CDS 55.0%
CAACAGTTGCCCTGCAAGTG+GGG - chr3.2:19983378-19983397 None:intergenic 55.0%
GGAATTGCTGGGCTTTACCG+TGG + chr3.2:19984042-19984061 MS.gene60471:CDS 55.0%
TAGGCTCTCTGAGCCCTACA+AGG + chr3.2:19983579-19983598 MS.gene60471:CDS 55.0%
! TTTGGGACATGGCAGGATGG+CGG - chr3.2:19983306-19983325 None:intergenic 55.0%
!! GGTGTTGTTGCTCTCTGGAG+AGG + chr3.2:19983640-19983659 MS.gene60471:CDS 55.0%
ATCCAATGGCCCCCACTTGC+AGG + chr3.2:19983365-19983384 MS.gene60471:CDS 60.0%
GACCAGATCTACGGTGGAGC+TGG - chr3.2:19983249-19983268 None:intergenic 60.0%
GAGCCATCCAAGCGCAAAGC+TGG - chr3.2:19984316-19984335 None:intergenic 60.0%
GCCGGTAACTTGGCATCTGG+AGG + chr3.2:19983871-19983890 MS.gene60471:CDS 60.0%
TCCAATGGCCCCCACTTGCA+GGG + chr3.2:19983366-19983385 MS.gene60471:CDS 60.0%
TCGCCAGCTTTGCGCTTGGA+TGG + chr3.2:19984310-19984329 MS.gene60471:CDS 60.0%
TGCTGGAGCAGCGACGAAGA+CGG - chr3.2:19983431-19983450 None:intergenic 60.0%
! AACATCCTCCGTGCCGTTGC+TGG + chr3.2:19984498-19984517 MS.gene60471:CDS 60.0%
!! GCTGGTGCTGGTGTGCTTTC+TGG + chr3.2:19984516-19984535 MS.gene60471:CDS 60.0%
!! TTGGCATCTGGAGGTGCTGC+TGG + chr3.2:19983880-19983899 MS.gene60471:CDS 60.0%
CACCAGCACCAGCAACGGCA+CGG - chr3.2:19984509-19984528 None:intergenic 65.0%
GCTGGAGCAGCGACGAAGAC+GGG - chr3.2:19983430-19983449 None:intergenic 65.0%
GGCCAGCTCCACCGTAGATC+TGG + chr3.2:19983244-19983263 MS.gene60471:CDS 65.0%
! GGACATGGCAGGATGGCGGT+AGG - chr3.2:19983302-19983321 None:intergenic 65.0%
CAGCACCAGCAACGGCACGG+AGG - chr3.2:19984506-19984525 None:intergenic 70.0%
GCCCTGCAAGTGGGGGCCAT+TGG - chr3.2:19983370-19983389 None:intergenic 70.0%
! CTCCGTGCCGTTGCTGGTGC+TGG + chr3.2:19984504-19984523 MS.gene60471:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 19983199 19984596 19983199 ID=MS.gene60471
chr3.2 mRNA 19983199 19984596 19983199 ID=MS.gene60471.t1;Parent=MS.gene60471
chr3.2 exon 19983199 19983699 19983199 ID=MS.gene60471.t1.exon1;Parent=MS.gene60471.t1
chr3.2 CDS 19983199 19983699 19983199 ID=cds.MS.gene60471.t1;Parent=MS.gene60471.t1
chr3.2 exon 19983790 19984161 19983790 ID=MS.gene60471.t1.exon2;Parent=MS.gene60471.t1
chr3.2 CDS 19983790 19984161 19983790 ID=cds.MS.gene60471.t1;Parent=MS.gene60471.t1
chr3.2 exon 19984300 19984596 19984300 ID=MS.gene60471.t1.exon3;Parent=MS.gene60471.t1
chr3.2 CDS 19984300 19984596 19984300 ID=cds.MS.gene60471.t1;Parent=MS.gene60471.t1
Gene Sequence

>MS.gene60471

ATGGTTGATCAGGTTAAGCAACCAACAATCATGGAGAAGGTCACTGGCCAGCTCCACCGTAGATCTGGTCTTTCATCTGGATTTAGGGGTTATGAAGGAACCTACCGCCATCCTGCCATGTCCCAAAGATTCTCCTATGGGAACTACTCAAACGCTGCATTGCAGAATCCAATGGCCCCCACTTGCAGGGCAACTGTTGATTTGTCAGCTGTTGCAACAACTGCTTCTCCCGTCTTCGTCGCTGCTCCAGCAGAAAAGAGCCATTTCGTTATTGACTTTCTCATGGGTGGTGTTTCAGCTGCCGTTTCCAAAACTGCTGCTGCTCCCATTGAACGTATTAAGCTTTTGATCCAAAACCAGGATGAGATGATCAAGTCTGGTAGGCTCTCTGAGCCCTACAAGGGTATTGGTGATTGTTTTAAAAGAACAATGGCTGATGAGGGTGTTGTTGCTCTCTGGAGAGGAAACACTGCCAATGTTATCCGTTATTTCCCCACTCAGGTTAGTTGTTTTCTAGTTTTGAATTTGACTCCTGTCCAATTGTTTTGTGTATTTAATGTGTTTCTGAATTTGCTATTTAAATTGTTTTAGGCTTTGAACTTTGCATTCAAAGATTACTTCAAGAGGCTTTTCAACTTCAAGAAGGACAGGGATGGTTACTGGAAATGGTTTGCCGGTAACTTGGCATCTGGAGGTGCTGCTGGTGCCTCATCCCTCTTGTTTGTTTACTCTCTTGATTATGCCCGTACTCGTCTTGCCAATGATTCTAAGGCAGCTAAGAAAGGTGGAGAAAGGCAGTTCAATGGTCTTGTTGATGTCTACAAGAAGACTCTTGCTACTGATGGAATTGCTGGGCTTTACCGTGGTTTCAACATCTCCTGTGTTGGAATCATTGTCTACCGTGGTCTGTACTTCGGATTGTATGATTCCCTCAAACCAGTTCTTCTCACTGGAAAATTGCAGGTTTGTTTTTTTTCCCTTTGAACAAATGATATTCATTTTAACCAAATTATTATTTTTCGATGCAAGCATGCAACTGCAAGATACTTTAGATTTTTATTCAATCATCTGCTGAAATCTTTTGAACTCTTGACATTACAGGATAGCTTTTTCGCCAGCTTTGCGCTTGGATGGCTCATTACCAATGGTGCAGGTCTAGCATCATACCCTATTGACACTGTTAGGAGAAGAATGATGATGACATCCGGAGAAGCTGTTAAGTACAAGAGTTCCTTTGATGCATTCCAACAAATCCTCAAGAATGAGGGTGCTAAGTCATTGTTCAAGGGAGCTGGTGCTAACATCCTCCGTGCCGTTGCTGGTGCTGGTGTGCTTTCTGGTTACGACAAGTTGCAGGTTCTTGTGTTCGGCAAGAAATACGGATCTGGTGGTGCTTAA
Protein sequence

>MS.gene60471.t1

MVDQVKQPTIMEKVTGQLHRRSGLSSGFRGYEGTYRHPAMSQRFSYGNYSNAALQNPMAPTCRATVDLSAVATTASPVFVAAPAEKSHFVIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSEPYKGIGDCFKRTMADEGVVALWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDRDGYWKWFAGNLASGGAAGASSLLFVYSLDYARTRLANDSKAAKKGGERQFNGLVDVYKKTLATDGIAGLYRGFNISCVGIIVYRGLYFGLYDSLKPVLLTGKLQDSFFASFALGWLITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSFDAFQQILKNEGAKSLFKGAGANILRAVAGAGVLSGYDKLQVLVFGKKYGSGGA