AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene006614

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006614.t1 MTR_4g078545 97.574 371 8 1 1 370 1 371 0.0 735
MS.gene006614.t1 MTR_3g028590 74.425 391 75 4 1 369 5 392 0.0 546
MS.gene006614.t1 MTR_3g028590 74.425 391 75 4 1 369 1 388 0.0 545
MS.gene006614.t1 MTR_8g036880 74.332 374 81 5 1 369 35 398 0.0 519
MS.gene006614.t1 MTR_2g098070 72.973 370 89 4 1 369 2 361 4.82e-180 504
MS.gene006614.t1 MTR_8g107270 58.188 287 120 0 73 359 17 303 8.41e-128 370
MS.gene006614.t1 MTR_3g104500 40.136 294 165 6 70 357 30 318 7.66e-69 219
MS.gene006614.t1 MTR_6g091800 29.431 299 190 6 70 357 15 303 2.16e-34 129
MS.gene006614.t1 MTR_3g104600 30.990 313 174 10 70 357 31 326 6.93e-31 120
MS.gene006614.t1 MTR_6g091800 27.554 323 189 7 70 357 15 327 2.68e-30 119
MS.gene006614.t1 MTR_1g012030 29.568 301 185 10 65 360 132 410 4.74e-30 119
MS.gene006614.t1 MTR_1g012030 27.273 209 131 6 62 267 221 411 1.38e-14 75.1
MS.gene006614.t1 MTR_6g091800 30.159 252 159 4 70 314 15 256 3.16e-29 114
MS.gene006614.t1 MTR_6g091800 31.020 245 152 4 70 307 15 249 5.58e-29 113
MS.gene006614.t1 MTR_6g091800 27.597 308 179 6 70 343 15 312 5.82e-29 114
MS.gene006614.t1 MTR_3g107510 28.881 277 173 8 77 349 125 381 1.73e-28 115
MS.gene006614.t1 MTR_3g107510 28.571 196 120 5 75 267 216 394 9.16e-16 78.6
MS.gene006614.t1 MTR_7g068340 31.095 283 164 8 85 360 98 356 2.31e-26 108
MS.gene006614.t1 MTR_5g009500 28.660 321 175 12 74 360 38 338 2.94e-26 107
MS.gene006614.t1 MTR_5g009500 23.661 224 154 4 54 266 121 338 9.71e-11 62.8
MS.gene006614.t1 MTR_8g027975 29.195 298 174 10 73 344 50 336 1.60e-25 106
MS.gene006614.t1 MTR_8g027975 26.108 203 131 5 77 266 157 353 1.03e-11 65.9
MS.gene006614.t1 MTR_6g091800 27.899 276 158 5 70 314 15 280 2.66e-25 104
MS.gene006614.t1 MTR_6g091800 28.625 269 151 5 70 307 15 273 5.30e-25 103
MS.gene006614.t1 MTR_7g064980 28.571 308 201 8 64 360 15 314 5.56e-25 103
MS.gene006614.t1 MTR_4g114320 27.698 278 181 6 74 349 123 382 9.61e-25 104
MS.gene006614.t1 MTR_4g114320 28.502 207 126 5 60 263 204 391 8.10e-17 81.6
MS.gene006614.t1 MTR_6g034145 29.268 246 160 5 123 357 7 249 1.89e-24 101
MS.gene006614.t1 MTR_6g034145 27.317 205 133 7 65 263 55 249 1.82e-12 67.4
MS.gene006614.t1 MTR_6g092210 29.167 240 162 5 123 357 7 243 8.53e-24 99.4
MS.gene006614.t1 MTR_6g092210 25.628 199 138 4 65 263 55 243 2.60e-12 66.6
MS.gene006614.t1 MTR_6g091800 31.694 183 109 3 70 245 15 188 1.22e-23 97.1
MS.gene006614.t1 MTR_5g081150 27.419 310 205 8 60 360 23 321 2.32e-23 99.4
MS.gene006614.t1 MTR_2g436930 27.673 318 185 8 71 354 12 318 4.76e-23 98.6
MS.gene006614.t1 MTR_8g096050 28.723 282 177 9 74 349 227 490 2.27e-22 98.6
MS.gene006614.t1 MTR_4g119770 26.398 322 190 8 71 356 11 321 5.87e-22 95.5
MS.gene006614.t1 MTR_7g084400 28.772 285 170 8 74 349 213 473 6.14e-22 97.4
MS.gene006614.t1 MTR_4g016730 31.597 288 163 11 74 354 89 349 1.56e-21 95.1
MS.gene006614.t1 MTR_3g098460 28.622 283 176 10 74 349 205 468 2.84e-20 92.4
MS.gene006614.t1 MTR_6g091800 28.986 207 107 4 70 245 15 212 1.03e-19 87.0
MS.gene006614.t1 MTR_4g119770 26.923 260 154 6 71 305 11 259 2.43e-19 87.0
MS.gene006614.t1 MTR_3g074490 27.597 308 176 12 77 360 119 403 1.75e-18 86.7
MS.gene006614.t1 MTR_4g005330 24.497 298 183 7 73 349 65 341 2.91e-17 82.4
MS.gene006614.t1 MTR_6g091910 28.283 198 131 5 70 263 15 205 1.00e-16 78.6
MS.gene006614.t1 MTR_6g091910 27.083 192 130 5 175 357 15 205 3.39e-11 62.8
MS.gene006614.t1 MTR_4g005330 24.490 294 180 7 77 349 3 275 3.33e-16 78.6
MS.gene006614.t1 MTR_1g090330 23.308 266 192 5 74 335 14 271 8.68e-16 77.4
MS.gene006614.t1 MTR_1g090330 29.167 168 111 4 75 241 112 272 4.92e-14 72.4
MS.gene006614.t1 MTR_5g030530 26.962 293 194 10 73 354 7 290 1.18e-15 77.0
MS.gene006614.t1 MTR_7g066690 23.507 268 196 6 70 335 13 273 4.94e-15 75.5
MS.gene006614.t1 MTR_7g084400 30.102 196 111 5 74 263 207 382 5.79e-15 76.3
MS.gene006614.t1 MTR_3g100570 24.573 293 190 8 74 342 6 291 8.10e-15 74.7
MS.gene006614.t1 MTR_5g081150 30.201 149 95 3 60 204 23 166 1.23e-14 73.2
MS.gene006614.t1 MTR_5g081150 30.201 149 95 3 60 204 23 166 1.52e-14 73.2
MS.gene006614.t1 MTR_8g066975 24.573 293 180 8 73 344 7 279 1.15e-13 71.2
MS.gene006614.t1 MTR_4g018750 24.060 266 189 8 74 335 15 271 1.45e-13 70.9
MS.gene006614.t1 MTR_2g102010 24.476 286 199 6 73 354 16 288 1.50e-13 70.9
MS.gene006614.t1 MTR_2g436930 29.082 196 112 4 180 354 39 228 3.17e-13 68.9
MS.gene006614.t1 MTR_7g109135 22.408 299 209 8 61 349 9 294 8.21e-13 68.9
MS.gene006614.t1 MTR_8g090210 25.000 308 184 12 77 360 113 397 9.99e-13 69.3
MS.gene006614.t1 MTR_4g057850 25.155 322 168 12 76 365 27 307 1.23e-12 68.6
MS.gene006614.t1 MTR_4g012850 25.692 253 174 5 119 364 51 296 1.53e-12 68.2
MS.gene006614.t1 MTR_4g012850 26.432 227 150 6 36 260 75 286 4.06e-11 63.5
MS.gene006614.t1 MTR_4g018750 24.303 251 177 8 74 320 15 256 9.23e-12 65.5
MS.gene006614.t1 MTR_5g032420 21.782 303 195 8 74 342 6 300 7.86e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene006614.t1 AT4G28390 80.159 378 65 2 3 370 2 379 0.0 583
MS.gene006614.t1 AT4G28390 80.159 378 65 2 3 370 2 379 0.0 583
MS.gene006614.t1 AT5G13490 74.286 385 82 4 1 369 1 384 0.0 544
MS.gene006614.t1 AT5G13490 74.286 385 82 4 1 369 1 384 0.0 544
MS.gene006614.t1 AT3G08580 74.278 381 85 4 1 369 1 380 0.0 527
MS.gene006614.t1 AT3G08580 74.278 381 85 4 1 369 1 380 0.0 527
MS.gene006614.t1 AT5G17400 61.644 292 112 0 70 361 10 301 3.71e-132 380
MS.gene006614.t1 AT5G56450 38.284 303 168 7 70 360 30 325 3.93e-62 202
MS.gene006614.t1 AT4G26180 33.225 307 175 9 70 360 17 309 1.22e-33 127
MS.gene006614.t1 AT4G26180 33.564 289 163 8 70 343 17 291 2.76e-32 123
MS.gene006614.t1 AT2G37890 29.641 334 216 8 37 360 8 332 3.16e-32 124
MS.gene006614.t1 AT3G55640 28.814 295 193 7 73 358 37 323 1.68e-30 119
MS.gene006614.t1 AT3G55640 28.814 295 193 7 73 358 37 323 1.68e-30 119
MS.gene006614.t1 AT1G14560 29.893 281 171 6 85 349 38 308 4.08e-30 118
MS.gene006614.t1 AT1G14560 30.182 275 166 6 85 343 38 302 2.59e-29 115
MS.gene006614.t1 AT3G51870 32.979 282 160 10 85 360 102 360 2.18e-28 114
MS.gene006614.t1 AT3G53940 29.870 308 195 9 64 360 63 360 4.24e-28 113
MS.gene006614.t1 AT4G32400 30.605 281 169 9 74 349 114 373 1.50e-27 112
MS.gene006614.t1 AT4G32400 26.020 196 125 5 75 267 208 386 3.84e-12 67.4
MS.gene006614.t1 AT5G01500 33.212 274 158 11 85 354 130 382 3.66e-27 111
MS.gene006614.t1 AT4G01100 27.974 311 186 10 77 360 45 344 1.05e-24 103
MS.gene006614.t1 AT5G51050 29.643 280 174 7 74 349 223 483 8.08e-24 102
MS.gene006614.t1 AT4G01100 27.385 325 184 11 77 360 45 358 2.20e-23 100
MS.gene006614.t1 AT4G01100 27.213 305 184 10 83 360 1 294 1.03e-22 97.4
MS.gene006614.t1 AT4G01100 25.854 205 129 6 77 266 98 294 6.65e-12 66.2
MS.gene006614.t1 AT5G61810 27.857 280 174 7 74 349 65 320 1.92e-22 97.1
MS.gene006614.t1 AT5G48970 27.673 318 188 8 71 354 19 328 2.30e-22 97.1
MS.gene006614.t1 AT3G21390 26.349 315 195 7 71 354 16 324 4.08e-22 96.3
MS.gene006614.t1 AT3G20240 25.085 295 185 6 73 349 51 327 4.46e-22 96.3
MS.gene006614.t1 AT3G21390 26.349 315 195 7 71 354 38 346 8.51e-22 95.5
MS.gene006614.t1 AT3G55640 28.718 195 127 3 73 263 37 223 1.06e-21 93.6
MS.gene006614.t1 AT3G55640 28.718 195 127 3 73 263 37 223 1.06e-21 93.6
MS.gene006614.t1 AT5G61810 27.857 280 174 7 74 349 208 463 2.03e-21 95.9
MS.gene006614.t1 AT3G53940 29.630 216 138 4 64 274 63 269 1.15e-19 88.6
MS.gene006614.t1 AT1G14560 31.383 188 112 4 178 349 24 210 1.33e-19 87.0
MS.gene006614.t1 AT5G07320 26.296 270 173 7 83 349 218 464 1.81e-19 90.1
MS.gene006614.t1 AT5G48970 26.953 256 152 6 126 354 10 257 2.63e-16 78.6
MS.gene006614.t1 AT5G64970 27.458 295 170 11 77 349 138 410 2.44e-15 77.4
MS.gene006614.t1 AT3G54110 23.485 264 193 7 74 335 15 271 5.59e-15 75.1
MS.gene006614.t1 AT1G14140 24.820 278 192 7 64 335 8 274 7.20e-15 75.1
MS.gene006614.t1 AT5G64970 26.780 295 172 11 77 349 14 286 1.12e-14 74.3
MS.gene006614.t1 AT5G64970 27.778 288 164 11 77 342 138 403 2.12e-14 74.3
MS.gene006614.t1 AT5G58970 24.460 278 195 6 70 342 12 279 2.60e-14 73.2
MS.gene006614.t1 AT5G58970 27.919 197 123 7 175 357 12 203 7.51e-11 63.2
MS.gene006614.t1 AT1G78180 27.273 308 177 12 77 360 131 415 2.25e-13 71.2
MS.gene006614.t1 AT5G66380 27.805 205 124 6 172 364 5 197 1.79e-12 67.8
MS.gene006614.t1 AT4G27940 25.368 272 168 12 114 360 146 407 2.44e-11 65.1
MS.gene006614.t1 AT5G46800 24.830 294 202 8 73 354 7 293 2.60e-11 64.3
MS.gene006614.t1 AT5G46800 24.830 294 202 8 73 354 7 293 2.60e-11 64.3
MS.gene006614.t1 AT5G46800 24.830 294 202 8 73 354 7 293 2.60e-11 64.3
MS.gene006614.t1 AT5G58970 27.919 197 123 7 175 357 12 203 5.84e-11 63.2

Find 101 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCCTATTTGTCTATTCTT+TGG 0.187425 4.4:+32033393 MS.gene006614:CDS
GATAGGTGGACATTCTTATT+TGG 0.215860 4.4:+32032384 MS.gene006614:CDS
TGAGCATTTCATCCTGGTTT+TGG 0.229183 4.4:-32032639 None:intergenic
GACATTCTTATTTGGTTTCC+AGG 0.255723 4.4:+32032392 MS.gene006614:CDS
GTCTTGTTTGGAAAGAAATA+TGG 0.260053 4.4:+32034378 MS.gene006614:CDS
GGTGGTGAGAGGCAGTTTAA+TGG 0.306537 4.4:+32033463 MS.gene006614:CDS
GCAGGTGCTGGTGTGCTTGC+TGG 0.309350 4.4:+32034336 MS.gene006614:CDS
TATCACAATTGGTGCTGGTT+TGG 0.315135 4.4:+32034155 MS.gene006614:CDS
AATCCAAAGAATAGACAAAT+AGG 0.321819 4.4:-32033396 None:intergenic
CAGTCACCAATTCCCTTATA+TGG 0.327603 4.4:-32032682 None:intergenic
TGCTAGTTTCCTGTTGGGAT+GGG 0.328388 4.4:+32034131 MS.gene006614:CDS
AATTATACCCTCATCCTTCA+TGG 0.333517 4.4:-32032716 None:intergenic
TTAGTATCTGGTGGAGCTGC+TGG 0.333758 4.4:+32033361 MS.gene006614:CDS
AACATATTGCGTGCTGTTGC+AGG 0.340667 4.4:+32034318 MS.gene006614:CDS
TTCTTTGCTAGTTTCCTGTT+GGG 0.343196 4.4:+32034126 MS.gene006614:CDS
CCTATCTTCTGAGCAATTGA+TGG 0.352718 4.4:-32032367 None:intergenic
AGAAGAATGATGATGACTTC+TGG 0.353166 4.4:+32034204 MS.gene006614:CDS
AAGCTGCAGCTGGTCTTGTT+TGG 0.353609 4.4:+32034366 MS.gene006614:CDS
TTACAGTCTGATGGCATTGC+TGG 0.359420 4.4:+32033511 MS.gene006614:CDS
AGCAATTTAATTCGTTCAAT+AGG 0.374655 4.4:-32032613 None:intergenic
GGCTACTGGAAGTGGTTTGC+TGG 0.374984 4.4:+32033334 MS.gene006614:CDS
TTTGGAAAGAAATATGGTTC+TGG 0.380189 4.4:+32034384 MS.gene006614:CDS
CTTTGCGTCATTTGCCAAAC+GGG 0.396020 4.4:-32033428 None:intergenic
ACTAATGGAGTGCAGACTTC+TGG 0.400993 4.4:+32032463 MS.gene006614:CDS
TTGCTAGTTTCCTGTTGGGA+TGG 0.404433 4.4:+32034130 MS.gene006614:CDS
AGATGTTGAATCCACGATAA+AGG 0.405874 4.4:-32033534 None:intergenic
CCATCAATTGCTCAGAAGAT+AGG 0.406150 4.4:+32032367 MS.gene006614:CDS
GGATTATGCCCGTACCCGTT+TGG 0.408041 4.4:+32033414 MS.gene006614:CDS
CTGAAACCAGTTGTCTTGGT+TGG 0.408866 4.4:+32033610 MS.gene006614:CDS
TCTGAGCCATATAAGGGAAT+TGG 0.421286 4.4:+32032676 MS.gene006614:CDS
TCTTCTACGCACAGTGTCAA+TGG 0.421926 4.4:-32034187 None:intergenic
GTTAGAGTTGGCGCTAAGCC+TGG 0.422723 4.4:-32032410 None:intergenic
CAGGTTATCTGAGCCATATA+AGG 0.423186 4.4:+32032669 MS.gene006614:CDS
TAGTATCTGGTGGAGCTGCT+GGG 0.429291 4.4:+32033362 MS.gene006614:CDS
GTGTATCGTGGTCTTTACTT+TGG 0.436559 4.4:+32033574 MS.gene006614:CDS
TGGGGTATCACAATTGGTGC+TGG 0.437821 4.4:+32034150 MS.gene006614:CDS
TTTGCTGGGAATTTAGTATC+TGG 0.438983 4.4:+32033349 MS.gene006614:CDS
CCAAACCATCGTTGCAAAAG+AGG 0.440517 4.4:+32034263 MS.gene006614:CDS
TGGAGTGCAGACTTCTGGTT+TGG 0.442748 4.4:+32032468 MS.gene006614:CDS
TGACGCAAAGGCTGCAAAGA+AGG 0.445560 4.4:+32033441 MS.gene006614:CDS
TTGGGATGGGGTATCACAAT+TGG 0.445710 4.4:+32034144 MS.gene006614:CDS
TTTCTTTGCTAGTTTCCTGT+TGG 0.448910 4.4:+32034125 MS.gene006614:CDS
CGTCATTTGCCAAACGGGTA+CGG 0.451569 4.4:-32033423 None:intergenic
TTCTCTGAAACCAGTTGTCT+TGG 0.454364 4.4:+32033606 MS.gene006614:CDS
ACGTCCGGTACTTTCACTAA+TGG 0.459227 4.4:+32032448 MS.gene006614:CDS
CCAGCGGGTCCTTTCTCTGC+TGG 0.461085 4.4:-32032529 None:intergenic
TTGCGTGCTGTTGCAGGTGC+TGG 0.462273 4.4:+32034324 MS.gene006614:CDS
AAGAAATATGGTTCTGGCGG+CGG 0.463431 4.4:+32034390 MS.gene006614:CDS
GGTCCTTTCTCTGCTGGAGA+AGG 0.466249 4.4:-32032523 None:intergenic
AAACCAGTTGTCTTGGTTGG+TGG 0.469067 4.4:+32033613 MS.gene006614:CDS
TTGTGATACCCCATCCCAAC+AGG 0.471309 4.4:-32034140 None:intergenic
CACAGTGTCAATGGGATAAG+AGG 0.473276 4.4:-32034178 None:intergenic
ATTCAGATTATCACCTGAGT+AGG 0.479406 4.4:-32032781 None:intergenic
GCTAGTTTCCTGTTGGGATG+GGG 0.479609 4.4:+32034132 MS.gene006614:CDS
TGTCTTGGTTGGTGGGATGC+AGG 0.479765 4.4:+32033621 MS.gene006614:CDS
ATCCCACCAACCAAGACAAC+TGG 0.485200 4.4:-32033616 None:intergenic
AGGTTATCTGAGCCATATAA+GGG 0.488890 4.4:+32032670 MS.gene006614:CDS
CCTTTGCGTCATTTGCCAAA+CGG 0.490345 4.4:-32033429 None:intergenic
GTCATTTGCCAAACGGGTAC+GGG 0.492794 4.4:-32033422 None:intergenic
ACAGAGATGGCTACTGGAAG+TGG 0.495428 4.4:+32033326 MS.gene006614:CDS
GCTGGGAATTTAGTATCTGG+TGG 0.499128 4.4:+32033352 MS.gene006614:CDS
AGCAATGCCATCAGACTGTA+AGG 0.504971 4.4:-32033509 None:intergenic
TACAGGAATTACTCTACGTC+CGG 0.508953 4.4:+32032433 MS.gene006614:CDS
GTCCCTTCTCCAGCAGAGAA+AGG 0.510593 4.4:+32032520 MS.gene006614:CDS
CTTCTACGCACAGTGTCAAT+GGG 0.512712 4.4:-32034186 None:intergenic
GTAATTCCTGTAGTTAGAGT+TGG 0.521037 4.4:-32032422 None:intergenic
AACCAGTTGTCTTGGTTGGT+GGG 0.523148 4.4:+32033614 MS.gene006614:CDS
TGCTCGAACCATGAAGGATG+AGG 0.524575 4.4:+32032708 MS.gene006614:CDS
TGGTTATGACAAGCTGCAGC+TGG 0.524956 4.4:+32034356 MS.gene006614:CDS
GGCATTGCTGGCCTTTATCG+TGG 0.542365 4.4:+32033523 MS.gene006614:CDS
GTCCTTTCTCTGCTGGAGAA+GGG 0.549707 4.4:-32032522 None:intergenic
GCTACTGGAAGTGGTTTGCT+GGG 0.556481 4.4:+32033335 MS.gene006614:CDS
GGATTCAACATCTCGTGTGT+TGG 0.556905 4.4:+32033544 MS.gene006614:CDS
ATGAAATGCTCAAAAGTGGC+AGG 0.556927 4.4:+32032650 MS.gene006614:CDS
AGTATCTGGTGGAGCTGCTG+GGG 0.568550 4.4:+32033363 MS.gene006614:CDS
TTAGCGCCAACTCTAACTAC+AGG 0.570302 4.4:+32032416 MS.gene006614:CDS
TTCAGATTATCACCTGAGTA+GGG 0.571644 4.4:-32032780 None:intergenic
GACGCAAAGGCTGCAAAGAA+GGG 0.572621 4.4:+32033442 MS.gene006614:CDS
CCAGCAGAGAAAGGACCCGC+TGG 0.578869 4.4:+32032529 MS.gene006614:CDS
TGAGAGGCAGTTTAATGGAA+TGG 0.581022 4.4:+32033468 MS.gene006614:CDS
CAAACCATCGTTGCAAAAGA+GGG 0.586013 4.4:+32034264 MS.gene006614:CDS
CAGGATGAAATGCTCAAAAG+TGG 0.586952 4.4:+32032646 MS.gene006614:CDS
AAGAAAACCTTACAGTCTGA+TGG 0.592392 4.4:+32033502 MS.gene006614:CDS
TAGGCTGCAATCATGGCCGA+TGG 0.596371 4.4:+32032334 None:intergenic
CACTCCATTAGTGAAAGTAC+CGG 0.601548 4.4:-32032452 None:intergenic
TAAATTGCTTATCCAAAACC+AGG 0.601587 4.4:+32032627 MS.gene006614:CDS
GGTATAATTGCTCTGTGGAG+AGG 0.606231 4.4:+32032730 MS.gene006614:CDS
GCTCGAACCATGAAGGATGA+GGG 0.608674 4.4:+32032709 MS.gene006614:CDS
TGATGGATGATGTGGTCCAT+CGG 0.611029 4.4:-32032350 None:intergenic
GGAAAGAAATATGGTTCTGG+CGG 0.618520 4.4:+32034387 MS.gene006614:CDS
TCAATTGCTCAGAAGATAGG+TGG 0.621358 4.4:+32032370 MS.gene006614:CDS
CTGCAAAGAAGGGTGGTGAG+AGG 0.621915 4.4:+32033452 MS.gene006614:CDS
AGAATAGACAAATAGGAGTG+AGG 0.622653 4.4:-32033389 None:intergenic
CCGTTTGGCAAATGACGCAA+AGG 0.622778 4.4:+32033429 MS.gene006614:CDS
TATCAGATACTTCCCTACTC+AGG 0.622842 4.4:+32032768 MS.gene006614:CDS
TGAGCAATTGATGGATGATG+TGG 0.652110 4.4:-32032358 None:intergenic
ATAGACAAATAGGAGTGAGG+AGG 0.664089 4.4:-32033386 None:intergenic
GTTGGAATTATAGTGTATCG+TGG 0.683295 4.4:+32033562 MS.gene006614:CDS
GCAAAGGCTGCAAAGAAGGG+TGG 0.689413 4.4:+32033445 MS.gene006614:CDS
ATGAGGGTATAATTGCTCTG+TGG 0.733403 4.4:+32032725 MS.gene006614:CDS
GGGACAGTAAATGACGACAG+AGG 0.743353 4.4:-32032502 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATGAAATTAGAAATTAA+AGG - chr4.4:32033240-32033259 None:intergenic 15.0%
!! ATGAACATTACTAAAATAAT+TGG - chr4.4:32034062-32034081 None:intergenic 15.0%
!! TGAACATTACTAAAATAATT+GGG - chr4.4:32034061-32034080 None:intergenic 15.0%
!!! TGTACAAAATTTGATTTTAA+CGG + chr4.4:32033646-32033665 MS.gene006614:intron 15.0%
!! GAACATTACTAAAATAATTG+GGG - chr4.4:32034060-32034079 None:intergenic 20.0%
!! TTGAAACTTTAATTGAACTA+GGG + chr4.4:32033999-32034018 MS.gene006614:intron 20.0%
!!! AATTTCATTCTTTTCCTTTT+AGG + chr4.4:32033249-32033268 MS.gene006614:intron 20.0%
!!! AATTTTAAAAAAGACAGAGA+TGG + chr4.4:32033313-32033332 MS.gene006614:CDS 20.0%
!!! ATTGAGATTAGCTTATATTT+TGG + chr4.4:32033060-32033079 MS.gene006614:intron 20.0%
!!! CATCATTTTCTTATTATCTA+TGG + chr4.4:32034079-32034098 MS.gene006614:intron 20.0%
!!! GGAATAATTTTAATCTAACA+CGG - chr4.4:32033697-32033716 None:intergenic 20.0%
!!! GTACAAAATTTGATTTTAAC+GGG + chr4.4:32033647-32033666 MS.gene006614:intron 20.0%
!!! TAAAATTATTCCTCTTTTGT+TGG + chr4.4:32033705-32033724 MS.gene006614:intron 20.0%
!!! TTTTAATAGAAAGATGTCTA+GGG - chr4.4:32033944-32033963 None:intergenic 20.0%
! AAATGCAAGGATACAAAAAT+AGG - chr4.4:32033109-32033128 None:intergenic 25.0%
! AATCCAAAGAATAGACAAAT+AGG - chr4.4:32033399-32033418 None:intergenic 25.0%
! AGAAATTTGTGGAAATCTAA+TGG + chr4.4:32032907-32032926 MS.gene006614:intron 25.0%
! AGCAATTTAATTCGTTCAAT+AGG - chr4.4:32032616-32032635 None:intergenic 25.0%
! ATAAGTTATATGCGTTGTAA+GGG + chr4.4:32032852-32032871 MS.gene006614:intron 25.0%
! CTAAAAGAAAGTCAAAATTG+TGG - chr4.4:32034037-32034056 None:intergenic 25.0%
! CTTGAAACTTTAATTGAACT+AGG + chr4.4:32033998-32034017 MS.gene006614:intron 25.0%
! TTAGATTTCCACAAATTTCT+TGG - chr4.4:32032907-32032926 None:intergenic 25.0%
!! AGAAAATGTTTTGTATGTCT+AGG - chr4.4:32033137-32033156 None:intergenic 25.0%
!! GAAAATGTTTTGTATGTCTA+GGG - chr4.4:32033136-32033155 None:intergenic 25.0%
!!! ACTTTGATTTTATGTTTGTC+AGG + chr4.4:32033732-32033751 MS.gene006614:intron 25.0%
!!! AGATTAGCTTATATTTTGGA+AGG + chr4.4:32033064-32033083 MS.gene006614:intron 25.0%
!!! ATTTTTGATCGATTTTCTGA+TGG + chr4.4:32032552-32032571 MS.gene006614:CDS 25.0%
!!! GATTAGCTTATATTTTGGAA+GGG + chr4.4:32033065-32033084 MS.gene006614:intron 25.0%
!!! GTAAAAGGTGTTTAATAGAA+AGG - chr4.4:32033922-32033941 None:intergenic 25.0%
!!! GTTTTAATAGAAAGATGTCT+AGG - chr4.4:32033945-32033964 None:intergenic 25.0%
!!! TTTGATTTTAACGGGAAATA+TGG + chr4.4:32033655-32033674 MS.gene006614:intron 25.0%
!!! TTTTTGATCGATTTTCTGAT+GGG + chr4.4:32032553-32032572 MS.gene006614:CDS 25.0%
AAAATCGATCAAAAATCCAG+CGG - chr4.4:32032548-32032567 None:intergenic 30.0%
ACTCCTATTTGTCTATTCTT+TGG + chr4.4:32033393-32033412 MS.gene006614:CDS 30.0%
CAAAGTAAAACCAACAAAAG+AGG - chr4.4:32033718-32033737 None:intergenic 30.0%
CATAAGTTATATGCGTTGTA+AGG + chr4.4:32032851-32032870 MS.gene006614:intron 30.0%
CTTACAACGCATATAACTTA+TGG - chr4.4:32032853-32032872 None:intergenic 30.0%
GTGTTAAACCAAGAAATTTG+TGG + chr4.4:32032896-32032915 MS.gene006614:intron 30.0%
TAAATTGCTTATCCAAAACC+AGG + chr4.4:32032627-32032646 MS.gene006614:CDS 30.0%
TTAACACAATATACACTTCG+AGG - chr4.4:32032883-32032902 None:intergenic 30.0%
TTTAGCAATCTATGATGTCT+AGG - chr4.4:32033978-32033997 None:intergenic 30.0%
TTTGGAAAGAAATATGGTTC+TGG + chr4.4:32034384-32034403 MS.gene006614:CDS 30.0%
! ATATACACATTACTGATTGC+AGG - chr4.4:32033894-32033913 None:intergenic 30.0%
! ATGGTACTTTTCTTGTTAAC+AGG + chr4.4:32034098-32034117 MS.gene006614:intron 30.0%
! CTATGATGTCTAGGATATAA+AGG - chr4.4:32033969-32033988 None:intergenic 30.0%
!! CTTTTAAGGATTACTTCAAG+AGG + chr4.4:32033284-32033303 MS.gene006614:CDS 30.0%
!! GTCTTGTTTGGAAAGAAATA+TGG + chr4.4:32034378-32034397 MS.gene006614:CDS 30.0%
AAAAACTGTCACTGTGATTC+CGG + chr4.4:32032975-32032994 MS.gene006614:intron 35.0%
AAATCGATCAAAAATCCAGC+GGG - chr4.4:32032547-32032566 None:intergenic 35.0%
AAGAAAACCTTACAGTCTGA+TGG + chr4.4:32033502-32033521 MS.gene006614:CDS 35.0%
AATTATACCCTCATCCTTCA+TGG - chr4.4:32032719-32032738 None:intergenic 35.0%
AGAATAGACAAATAGGAGTG+AGG - chr4.4:32033392-32033411 None:intergenic 35.0%
AGATGTTGAATCCACGATAA+AGG - chr4.4:32033537-32033556 None:intergenic 35.0%
AGGTTATCTGAGCCATATAA+GGG + chr4.4:32032670-32032689 MS.gene006614:CDS 35.0%
ATTCAGATTATCACCTGAGT+AGG - chr4.4:32032784-32032803 None:intergenic 35.0%
GATAGGTGGACATTCTTATT+TGG + chr4.4:32032384-32032403 MS.gene006614:CDS 35.0%
GTAATTCCTGTAGTTAGAGT+TGG - chr4.4:32032425-32032444 None:intergenic 35.0%
GTTGGAATTATAGTGTATCG+TGG + chr4.4:32033562-32033581 MS.gene006614:CDS 35.0%
TGTGGTGTTTGTATAAGTAG+AGG - chr4.4:32032952-32032971 None:intergenic 35.0%
TTCAGATTATCACCTGAGTA+GGG - chr4.4:32032783-32032802 None:intergenic 35.0%
TTTGCTGGGAATTTAGTATC+TGG + chr4.4:32033349-32033368 MS.gene006614:CDS 35.0%
! ACAGTGACAGTTTTTGTATG+TGG - chr4.4:32032970-32032989 None:intergenic 35.0%
! AGAAAGATGTCTAGGGTAAA+AGG - chr4.4:32033937-32033956 None:intergenic 35.0%
! AGAAGAATGATGATGACTTC+TGG + chr4.4:32034204-32034223 MS.gene006614:CDS 35.0%
! GACATTCTTATTTGGTTTCC+AGG + chr4.4:32032392-32032411 MS.gene006614:CDS 35.0%
! GGTACTAAGTCTCTTTTCAA+AGG + chr4.4:32034285-32034304 MS.gene006614:CDS 35.0%
! TTCTTTGCTAGTTTCCTGTT+GGG + chr4.4:32034126-32034145 MS.gene006614:CDS 35.0%
! TTTCCATGTGTTGTCTTGTT+TGG + chr4.4:32033009-32033028 MS.gene006614:intron 35.0%
! TTTCTTTGCTAGTTTCCTGT+TGG + chr4.4:32034125-32034144 MS.gene006614:CDS 35.0%
!! CTCTTTTGGTGATTGAATGT+TGG + chr4.4:32033191-32033210 MS.gene006614:intron 35.0%
!! TTCCATGTGTTGTCTTGTTT+GGG + chr4.4:32033010-32033029 MS.gene006614:intron 35.0%
AAAAAGACAGAGATGGCTAC+TGG + chr4.4:32033320-32033339 MS.gene006614:CDS 40.0%
AACCCAAACAAGACAACACA+TGG - chr4.4:32033015-32033034 None:intergenic 40.0%
AGCAAAGTTCAGAGCCTAAA+AGG - chr4.4:32033266-32033285 None:intergenic 40.0%
ATAGACAAATAGGAGTGAGG+AGG - chr4.4:32033389-32033408 None:intergenic 40.0%
ATGAAATGCTCAAAAGTGGC+AGG + chr4.4:32032650-32032669 MS.gene006614:CDS 40.0%
CAAACCATCGTTGCAAAAGA+GGG + chr4.4:32034264-32034283 MS.gene006614:CDS 40.0%
CAGGATGAAATGCTCAAAAG+TGG + chr4.4:32032646-32032665 MS.gene006614:CDS 40.0%
CAGGTTATCTGAGCCATATA+AGG + chr4.4:32032669-32032688 MS.gene006614:CDS 40.0%
CAGTCACCAATTCCCTTATA+TGG - chr4.4:32032685-32032704 None:intergenic 40.0%
CATGGAAAATCTCATGACAC+CGG - chr4.4:32032997-32033016 None:intergenic 40.0%
CCATCAATTGCTCAGAAGAT+AGG + chr4.4:32032367-32032386 MS.gene006614:CDS 40.0%
CCTATCTTCTGAGCAATTGA+TGG - chr4.4:32032370-32032389 None:intergenic 40.0%
GTGTATCGTGGTCTTTACTT+TGG + chr4.4:32033574-32033593 MS.gene006614:CDS 40.0%
TACAGGAATTACTCTACGTC+CGG + chr4.4:32032433-32032452 MS.gene006614:CDS 40.0%
TATCAGATACTTCCCTACTC+AGG + chr4.4:32032768-32032787 MS.gene006614:CDS 40.0%
TCAATTGCTCAGAAGATAGG+TGG + chr4.4:32032370-32032389 MS.gene006614:CDS 40.0%
TCTGAGCCATATAAGGGAAT+TGG + chr4.4:32032676-32032695 MS.gene006614:CDS 40.0%
TGAGAGGCAGTTTAATGGAA+TGG + chr4.4:32033468-32033487 MS.gene006614:CDS 40.0%
TGTCTAGGGATGTAAATGCA+AGG - chr4.4:32033122-32033141 None:intergenic 40.0%
! ATGAGGGTATAATTGCTCTG+TGG + chr4.4:32032725-32032744 MS.gene006614:CDS 40.0%
! CACTCCATTAGTGAAAGTAC+CGG - chr4.4:32032455-32032474 None:intergenic 40.0%
! CACTTTTGAGCATTTCATCC+TGG - chr4.4:32032648-32032667 None:intergenic 40.0%
! GATGCATGTGAATGCTCTTT+TGG + chr4.4:32033177-32033196 MS.gene006614:intron 40.0%
! TGAGCAATTGATGGATGATG+TGG - chr4.4:32032361-32032380 None:intergenic 40.0%
! TGAGCATTTCATCCTGGTTT+TGG - chr4.4:32032642-32032661 None:intergenic 40.0%
!! AAGTCTCTTTTCAAAGGTGC+TGG + chr4.4:32034291-32034310 MS.gene006614:CDS 40.0%
!! GGAAAGAAATATGGTTCTGG+CGG + chr4.4:32034387-32034406 MS.gene006614:CDS 40.0%
!! TATCACAATTGGTGCTGGTT+TGG + chr4.4:32034155-32034174 MS.gene006614:CDS 40.0%
!! TTCTCTGAAACCAGTTGTCT+TGG + chr4.4:32033606-32033625 MS.gene006614:CDS 40.0%
!! TTGATCGATTTTCTGATGGG+AGG + chr4.4:32032556-32032575 MS.gene006614:CDS 40.0%
!!! GGCTCTGAACTTTGCTTTTA+AGG + chr4.4:32033270-32033289 MS.gene006614:intron 40.0%
AAACCAGTTGTCTTGGTTGG+TGG + chr4.4:32033613-32033632 MS.gene006614:CDS 45.0%
AACCAGTTGTCTTGGTTGGT+GGG + chr4.4:32033614-32033633 MS.gene006614:CDS 45.0%
ACGTCCGGTACTTTCACTAA+TGG + chr4.4:32032448-32032467 MS.gene006614:CDS 45.0%
ACTAATGGAGTGCAGACTTC+TGG + chr4.4:32032463-32032482 MS.gene006614:CDS 45.0%
AGCAATGCCATCAGACTGTA+AGG - chr4.4:32033512-32033531 None:intergenic 45.0%
CACAGTGTCAATGGGATAAG+AGG - chr4.4:32034181-32034200 None:intergenic 45.0%
CCAAACCATCGTTGCAAAAG+AGG + chr4.4:32034263-32034282 MS.gene006614:CDS 45.0%
CCTTTGCGTCATTTGCCAAA+CGG - chr4.4:32033432-32033451 None:intergenic 45.0%
CTGAAACCAGTTGTCTTGGT+TGG + chr4.4:32033610-32033629 MS.gene006614:CDS 45.0%
CTTCTACGCACAGTGTCAAT+GGG - chr4.4:32034189-32034208 None:intergenic 45.0%
CTTTGCGTCATTTGCCAAAC+GGG - chr4.4:32033431-32033450 None:intergenic 45.0%
GCTGGGAATTTAGTATCTGG+TGG + chr4.4:32033352-32033371 MS.gene006614:CDS 45.0%
GGATTCAACATCTCGTGTGT+TGG + chr4.4:32033544-32033563 MS.gene006614:CDS 45.0%
GGTATAATTGCTCTGTGGAG+AGG + chr4.4:32032730-32032749 MS.gene006614:CDS 45.0%
TCTTCTACGCACAGTGTCAA+TGG - chr4.4:32034190-32034209 None:intergenic 45.0%
TTACAGTCTGATGGCATTGC+TGG + chr4.4:32033511-32033530 MS.gene006614:CDS 45.0%
TTAGCGCCAACTCTAACTAC+AGG + chr4.4:32032416-32032435 MS.gene006614:CDS 45.0%
! AACATATTGCGTGCTGTTGC+AGG + chr4.4:32034318-32034337 MS.gene006614:CDS 45.0%
! AGTACCCTCTTTTGCAACGA+TGG - chr4.4:32034271-32034290 None:intergenic 45.0%
! CCTCTTTTGCAACGATGGTT+TGG - chr4.4:32034266-32034285 None:intergenic 45.0%
! CTGTTTTGCTCGAACCATGA+AGG + chr4.4:32032702-32032721 MS.gene006614:CDS 45.0%
! TGATGGATGATGTGGTCCAT+CGG - chr4.4:32032353-32032372 None:intergenic 45.0%
! TGCTAGTTTCCTGTTGGGAT+GGG + chr4.4:32034131-32034150 MS.gene006614:CDS 45.0%
! TTGCTAGTTTCCTGTTGGGA+TGG + chr4.4:32034130-32034149 MS.gene006614:CDS 45.0%
! TTGGGATGGGGTATCACAAT+TGG + chr4.4:32034144-32034163 MS.gene006614:CDS 45.0%
!! AAGAAATATGGTTCTGGCGG+CGG + chr4.4:32034390-32034409 MS.gene006614:CDS 45.0%
ACAGAGATGGCTACTGGAAG+TGG + chr4.4:32033326-32033345 MS.gene006614:CDS 50.0%
ATCCCACCAACCAAGACAAC+TGG - chr4.4:32033619-32033638 None:intergenic 50.0%
CCGTTTGGCAAATGACGCAA+AGG + chr4.4:32033429-32033448 MS.gene006614:CDS 50.0%
CGTCATTTGCCAAACGGGTA+CGG - chr4.4:32033426-32033445 None:intergenic 50.0%
GACGCAAAGGCTGCAAAGAA+GGG + chr4.4:32033442-32033461 MS.gene006614:CDS 50.0%
GCTCGAACCATGAAGGATGA+GGG + chr4.4:32032709-32032728 MS.gene006614:CDS 50.0%
GGGACAGTAAATGACGACAG+AGG - chr4.4:32032505-32032524 None:intergenic 50.0%
GGTGGTGAGAGGCAGTTTAA+TGG + chr4.4:32033463-32033482 MS.gene006614:CDS 50.0%
GTCATTTGCCAAACGGGTAC+GGG - chr4.4:32033425-32033444 None:intergenic 50.0%
GTCCTTTCTCTGCTGGAGAA+GGG - chr4.4:32032525-32032544 None:intergenic 50.0%
TAGTATCTGGTGGAGCTGCT+GGG + chr4.4:32033362-32033381 MS.gene006614:CDS 50.0%
TGACGCAAAGGCTGCAAAGA+AGG + chr4.4:32033441-32033460 MS.gene006614:CDS 50.0%
TGCTCGAACCATGAAGGATG+AGG + chr4.4:32032708-32032727 MS.gene006614:CDS 50.0%
TGGTTATGACAAGCTGCAGC+TGG + chr4.4:32034356-32034375 MS.gene006614:CDS 50.0%
TTAGTATCTGGTGGAGCTGC+TGG + chr4.4:32033361-32033380 MS.gene006614:CDS 50.0%
TTGTGATACCCCATCCCAAC+AGG - chr4.4:32034143-32034162 None:intergenic 50.0%
! GCTAGTTTCCTGTTGGGATG+GGG + chr4.4:32034132-32034151 MS.gene006614:CDS 50.0%
! TGGAGTGCAGACTTCTGGTT+TGG + chr4.4:32032468-32032487 MS.gene006614:CDS 50.0%
! TGGGGTATCACAATTGGTGC+TGG + chr4.4:32034150-32034169 MS.gene006614:CDS 50.0%
!! AAGCTGCAGCTGGTCTTGTT+TGG + chr4.4:32034366-32034385 MS.gene006614:CDS 50.0%
!! GCTACTGGAAGTGGTTTGCT+GGG + chr4.4:32033335-32033354 MS.gene006614:CDS 50.0%
AGTATCTGGTGGAGCTGCTG+GGG + chr4.4:32033363-32033382 MS.gene006614:CDS 55.0%
CTGCAAAGAAGGGTGGTGAG+AGG + chr4.4:32033452-32033471 MS.gene006614:CDS 55.0%
GCAAAGGCTGCAAAGAAGGG+TGG + chr4.4:32033445-32033464 MS.gene006614:CDS 55.0%
GGATTATGCCCGTACCCGTT+TGG + chr4.4:32033414-32033433 MS.gene006614:CDS 55.0%
GGTCCTTTCTCTGCTGGAGA+AGG - chr4.4:32032526-32032545 None:intergenic 55.0%
GTCCCTTCTCCAGCAGAGAA+AGG + chr4.4:32032520-32032539 MS.gene006614:CDS 55.0%
GTTAGAGTTGGCGCTAAGCC+TGG - chr4.4:32032413-32032432 None:intergenic 55.0%
! GGCTACTGGAAGTGGTTTGC+TGG + chr4.4:32033334-32033353 MS.gene006614:CDS 55.0%
!! GGCATTGCTGGCCTTTATCG+TGG + chr4.4:32033523-32033542 MS.gene006614:CDS 55.0%
!! TGTCTTGGTTGGTGGGATGC+AGG + chr4.4:32033621-32033640 MS.gene006614:CDS 55.0%
! TTGCGTGCTGTTGCAGGTGC+TGG + chr4.4:32034324-32034343 MS.gene006614:CDS 60.0%
CCAGCAGAGAAAGGACCCGC+TGG + chr4.4:32032529-32032548 MS.gene006614:CDS 65.0%
CCAGCGGGTCCTTTCTCTGC+TGG - chr4.4:32032532-32032551 None:intergenic 65.0%
!! GCAGGTGCTGGTGTGCTTGC+TGG + chr4.4:32034336-32034355 MS.gene006614:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 32032346 32034416 32032346 ID=MS.gene006614
chr4.4 mRNA 32032346 32034416 32032346 ID=MS.gene006614.t1;Parent=MS.gene006614
chr4.4 exon 32032346 32032789 32032346 ID=MS.gene006614.t1.exon1;Parent=MS.gene006614.t1
chr4.4 CDS 32032346 32032789 32032346 ID=cds.MS.gene006614.t1;Parent=MS.gene006614.t1
chr4.4 exon 32033271 32033642 32033271 ID=MS.gene006614.t1.exon2;Parent=MS.gene006614.t1
chr4.4 CDS 32033271 32033642 32033271 ID=cds.MS.gene006614.t1;Parent=MS.gene006614.t1
chr4.4 exon 32034120 32034416 32034120 ID=MS.gene006614.t1.exon3;Parent=MS.gene006614.t1
chr4.4 CDS 32034120 32034416 32034120 ID=cds.MS.gene006614.t1;Parent=MS.gene006614.t1
Gene Sequence

>MS.gene006614

ATGGCCGATGGACCACATCATCCATCAATTGCTCAGAAGATAGGTGGACATTCTTATTTGGTTTCCAGGCTTAGCGCCAACTCTAACTACAGGAATTACTCTACGTCCGGTACTTTCACTAATGGAGTGCAGACTTCTGGTTTGGCACTTGCGTCACCTCTGTCGTCATTTACTGTCCCTTCTCCAGCAGAGAAAGGACCCGCTGGATTTTTGATCGATTTTCTGATGGGAGGAGTGTCTGCTGCTGTGTCAAAGACAGCTGCTGCTCCTATTGAACGAATTAAATTGCTTATCCAAAACCAGGATGAAATGCTCAAAAGTGGCAGGTTATCTGAGCCATATAAGGGAATTGGTGACTGTTTTGCTCGAACCATGAAGGATGAGGGTATAATTGCTCTGTGGAGAGGCAATACTGCTAATGTTATCAGATACTTCCCTACTCAGGTGATAATCTGAATTTTTTTCTTGTAAGATATTTTCTATTTTGACATATTCTTACAGCATCCATAAGTTATATGCGTTGTAAGGGTTGTTCCTCGAAGTGTATATTGTGTTAAACCAAGAAATTTGTGGAAATCTAATGGTTGAATATTCTTTTACTGACCTCTACTTATACAAACACCACATACAAAAACTGTCACTGTGATTCCGGTGTCATGAGATTTTCCATGTGTTGTCTTGTTTGGGTTTTGTTCATGCTGTCTTATTATATATATTGAGATTAGCTTATATTTTGGAAGGGAAAAGATTTTATATAATACCTATTTTTGTATCCTTGCATTTACATCCCTAGACATACAAAACATTTTCTCTCTTTTTTTATCTGATTTTGATGCATGTGAATGCTCTTTTGGTGATTGAATGTTGGTTCTGTAGTTTCTGCGAGCTGTTCCTTTAATTTCTAATTTCATTCTTTTCCTTTTAGGCTCTGAACTTTGCTTTTAAGGATTACTTCAAGAGGCTTTTCAATTTTAAAAAAGACAGAGATGGCTACTGGAAGTGGTTTGCTGGGAATTTAGTATCTGGTGGAGCTGCTGGGGCCTCCTCACTCCTATTTGTCTATTCTTTGGATTATGCCCGTACCCGTTTGGCAAATGACGCAAAGGCTGCAAAGAAGGGTGGTGAGAGGCAGTTTAATGGAATGGTTGATGTTTACAAGAAAACCTTACAGTCTGATGGCATTGCTGGCCTTTATCGTGGATTCAACATCTCGTGTGTTGGAATTATAGTGTATCGTGGTCTTTACTTTGGAATGTATGATTCTCTGAAACCAGTTGTCTTGGTTGGTGGGATGCAGGTATGTACAAAATTTGATTTTAACGGGAAATATGGTGTTTCTTAATCTGTTCCGTGTTAGATTAAAATTATTCCTCTTTTGTTGGTTTTACTTTGATTTTATGTTTGTCAGGCAATATTTTGTTGATTGTAAACGATTAGCTTGAATATTTAATTTTTTATGTCTTAAATATAATTTGAAAATGATGCTTTTAGAATGCTAATCTAGTTTATTAGCACATTATCTGTAAGCATGTTAGTATTCAGTTACCTGCAATCAGTAATGTGTATATATCTTCCTTTCTATTAAACACCTTTTACCCTAGACATCTTTCTATTAAAACACCTTTATATCCTAGACATCATAGATTGCTAAACTTGAAACTTTAATTGAACTAGGGAAATTTGTTCTTCCACAATTTTGACTTTCTTTTAGCCCCAATTATTTTAGTAATGTTCATCATTTTCTTATTATCTATGGTACTTTTCTTGTTAACAGGATAGTTTCTTTGCTAGTTTCCTGTTGGGATGGGGTATCACAATTGGTGCTGGTTTGGCCTCTTATCCCATTGACACTGTGCGTAGAAGAATGATGATGACTTCTGGAGAAGCTGTGAAGTATAACAGCTCTTTGCATGCATTCCAAACCATCGTTGCAAAAGAGGGTACTAAGTCTCTTTTCAAAGGTGCTGGTGCAAACATATTGCGTGCTGTTGCAGGTGCTGGTGTGCTTGCTGGTTATGACAAGCTGCAGCTGGTCTTGTTTGGAAAGAAATATGGTTCTGGCGGCGGCTAA
Protein sequence

>MS.gene006614.t1

MADGPHHPSIAQKIGGHSYLVSRLSANSNYRNYSTSGTFTNGVQTSGLALASPLSSFTVPSPAEKGPAGFLIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMLKSGRLSEPYKGIGDCFARTMKDEGIIALWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDRDGYWKWFAGNLVSGGAAGASSLLFVYSLDYARTRLANDAKAAKKGGERQFNGMVDVYKKTLQSDGIAGLYRGFNISCVGIIVYRGLYFGMYDSLKPVVLVGGMQDSFFASFLLGWGITIGAGLASYPIDTVRRRMMMTSGEAVKYNSSLHAFQTIVAKEGTKSLFKGAGANILRAVAGAGVLAGYDKLQLVLFGKKYGSGGG