AlfalfaGEDB | MS.gene065946

M. sativa cultivar XinJiangDaYe / MS.gene065946

KEGG: K05863
GO: GO:0003674 GO:0005215 GO:0005575 GO:0005743 GO:0006810 GO:0008150 GO:0016020 GO:0019866 GO:0022857 GO:0031090 GO:0031966 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00153.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065946.t1	XP_013456669.1	97.6	371	8	1	1	370	1	371	6.50E-203	716.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065946.t1	O49447	80.2	378	65	2	3	370	2	379	5.9e-168	591.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065946.t1	A0A072UXU6	97.6	371	8	1	1	370	1	371	4.7e-203	716.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049190	MS.gene065946	0.81282	3.50E-51	-1.69E-46
MS.gene049824	MS.gene065946	0.860051	2.70E-63	-1.69E-46
MS.gene049825	MS.gene065946	0.80134	9.43E-49	-1.69E-46
MS.gene050630	MS.gene065946	0.84403	9.73E-59	-1.69E-46
MS.gene050877	MS.gene065946	0.837517	4.96E-57	-1.69E-46
MS.gene052133	MS.gene065946	0.800335	1.51E-48	-1.69E-46
MS.gene052879	MS.gene065946	0.805724	1.16E-49	-1.69E-46
MS.gene053122	MS.gene065946	0.802962	4.37E-49	-1.69E-46
MS.gene055808	MS.gene065946	0.824213	9.12E-54	-1.69E-46
MS.gene056075	MS.gene065946	0.803727	3.04E-49	-1.69E-46
MS.gene057705	MS.gene065946	0.821936	3.10E-53	-1.69E-46
MS.gene06001	MS.gene065946	0.810633	1.05E-50	-1.69E-46
MS.gene060194	MS.gene065946	0.810963	8.87E-51	-1.69E-46
MS.gene060587	MS.gene065946	0.801708	7.93E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene065946	MS.gene040118	PPI
MS.gene065946	MS.gene82276	PPI
MS.gene56778	MS.gene065946	PPI
MS.gene065946	MS.gene90510	PPI
MS.gene041083	MS.gene065946	PPI
MS.gene015154	MS.gene065946	PPI
MS.gene66338	MS.gene065946	PPI
MS.gene074631	MS.gene065946	PPI
MS.gene065946	MS.gene60471	PPI
MS.gene065946	MS.gene016690	PPI
MS.gene010354	MS.gene065946	PPI
MS.gene75068	MS.gene065946	PPI
MS.gene065946	MS.gene94558	PPI
MS.gene065946	MS.gene32809	PPI
MS.gene065946	MS.gene23588	PPI
MS.gene79071	MS.gene065946	PPI
MS.gene064041	MS.gene065946	PPI
MS.gene065946	MS.gene46786	PPI
MS.gene065946	MS.gene012807	PPI
MS.gene065946	MS.gene011817	PPI
MS.gene047628	MS.gene065946	PPI
MS.gene015010	MS.gene065946	PPI
MS.gene065946	MS.gene93756	PPI
MS.gene030289	MS.gene065946	PPI
MS.gene37419	MS.gene065946	PPI
MS.gene064047	MS.gene065946	PPI
MS.gene065946	MS.gene074632	PPI
MS.gene064043	MS.gene065946	PPI
MS.gene065946	MS.gene046998	PPI
MS.gene99200	MS.gene065946	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065946.t1	MTR_4g078545	97.574	371	8	1	1	370	1	371	0.0	735
MS.gene065946.t1	MTR_3g028590	74.425	391	75	4	1	369	5	392	0.0	546
MS.gene065946.t1	MTR_3g028590	74.425	391	75	4	1	369	1	388	0.0	545
MS.gene065946.t1	MTR_8g036880	74.332	374	81	5	1	369	35	398	0.0	519
MS.gene065946.t1	MTR_2g098070	72.973	370	89	4	1	369	2	361	4.82e-180	504
MS.gene065946.t1	MTR_8g107270	58.188	287	120	0	73	359	17	303	8.41e-128	370
MS.gene065946.t1	MTR_3g104500	40.136	294	165	6	70	357	30	318	7.66e-69	219
MS.gene065946.t1	MTR_6g091800	29.431	299	190	6	70	357	15	303	2.16e-34	129
MS.gene065946.t1	MTR_3g104600	30.990	313	174	10	70	357	31	326	6.93e-31	120
MS.gene065946.t1	MTR_6g091800	27.554	323	189	7	70	357	15	327	2.68e-30	119
MS.gene065946.t1	MTR_1g012030	29.568	301	185	10	65	360	132	410	4.74e-30	119
MS.gene065946.t1	MTR_1g012030	27.273	209	131	6	62	267	221	411	1.38e-14	75.1
MS.gene065946.t1	MTR_6g091800	30.159	252	159	4	70	314	15	256	3.16e-29	114
MS.gene065946.t1	MTR_6g091800	31.020	245	152	4	70	307	15	249	5.58e-29	113
MS.gene065946.t1	MTR_6g091800	27.597	308	179	6	70	343	15	312	5.82e-29	114
MS.gene065946.t1	MTR_3g107510	28.881	277	173	8	77	349	125	381	1.73e-28	115
MS.gene065946.t1	MTR_3g107510	28.571	196	120	5	75	267	216	394	9.16e-16	78.6
MS.gene065946.t1	MTR_7g068340	31.095	283	164	8	85	360	98	356	2.31e-26	108
MS.gene065946.t1	MTR_5g009500	28.660	321	175	12	74	360	38	338	2.94e-26	107
MS.gene065946.t1	MTR_5g009500	23.661	224	154	4	54	266	121	338	9.71e-11	62.8
MS.gene065946.t1	MTR_8g027975	29.195	298	174	10	73	344	50	336	1.60e-25	106
MS.gene065946.t1	MTR_8g027975	26.108	203	131	5	77	266	157	353	1.03e-11	65.9
MS.gene065946.t1	MTR_6g091800	27.899	276	158	5	70	314	15	280	2.66e-25	104
MS.gene065946.t1	MTR_6g091800	28.625	269	151	5	70	307	15	273	5.30e-25	103
MS.gene065946.t1	MTR_7g064980	28.571	308	201	8	64	360	15	314	5.56e-25	103
MS.gene065946.t1	MTR_4g114320	27.698	278	181	6	74	349	123	382	9.61e-25	104
MS.gene065946.t1	MTR_4g114320	28.502	207	126	5	60	263	204	391	8.10e-17	81.6
MS.gene065946.t1	MTR_6g034145	29.268	246	160	5	123	357	7	249	1.89e-24	101
MS.gene065946.t1	MTR_6g034145	27.317	205	133	7	65	263	55	249	1.82e-12	67.4
MS.gene065946.t1	MTR_6g092210	29.167	240	162	5	123	357	7	243	8.53e-24	99.4
MS.gene065946.t1	MTR_6g092210	25.628	199	138	4	65	263	55	243	2.60e-12	66.6
MS.gene065946.t1	MTR_6g091800	31.694	183	109	3	70	245	15	188	1.22e-23	97.1
MS.gene065946.t1	MTR_5g081150	27.419	310	205	8	60	360	23	321	2.32e-23	99.4
MS.gene065946.t1	MTR_2g436930	27.673	318	185	8	71	354	12	318	4.76e-23	98.6
MS.gene065946.t1	MTR_8g096050	28.723	282	177	9	74	349	227	490	2.27e-22	98.6
MS.gene065946.t1	MTR_4g119770	26.398	322	190	8	71	356	11	321	5.87e-22	95.5
MS.gene065946.t1	MTR_7g084400	28.772	285	170	8	74	349	213	473	6.14e-22	97.4
MS.gene065946.t1	MTR_4g016730	31.597	288	163	11	74	354	89	349	1.56e-21	95.1
MS.gene065946.t1	MTR_3g098460	28.622	283	176	10	74	349	205	468	2.84e-20	92.4
MS.gene065946.t1	MTR_6g091800	28.986	207	107	4	70	245	15	212	1.03e-19	87.0
MS.gene065946.t1	MTR_4g119770	26.923	260	154	6	71	305	11	259	2.43e-19	87.0
MS.gene065946.t1	MTR_3g074490	27.597	308	176	12	77	360	119	403	1.75e-18	86.7
MS.gene065946.t1	MTR_4g005330	24.497	298	183	7	73	349	65	341	2.91e-17	82.4
MS.gene065946.t1	MTR_6g091910	28.283	198	131	5	70	263	15	205	1.00e-16	78.6
MS.gene065946.t1	MTR_6g091910	27.083	192	130	5	175	357	15	205	3.39e-11	62.8
MS.gene065946.t1	MTR_4g005330	24.490	294	180	7	77	349	3	275	3.33e-16	78.6
MS.gene065946.t1	MTR_1g090330	23.308	266	192	5	74	335	14	271	8.68e-16	77.4
MS.gene065946.t1	MTR_1g090330	29.167	168	111	4	75	241	112	272	4.92e-14	72.4
MS.gene065946.t1	MTR_5g030530	26.962	293	194	10	73	354	7	290	1.18e-15	77.0
MS.gene065946.t1	MTR_7g066690	23.507	268	196	6	70	335	13	273	4.94e-15	75.5
MS.gene065946.t1	MTR_7g084400	30.102	196	111	5	74	263	207	382	5.79e-15	76.3
MS.gene065946.t1	MTR_3g100570	24.573	293	190	8	74	342	6	291	8.10e-15	74.7
MS.gene065946.t1	MTR_5g081150	30.201	149	95	3	60	204	23	166	1.23e-14	73.2
MS.gene065946.t1	MTR_5g081150	30.201	149	95	3	60	204	23	166	1.52e-14	73.2
MS.gene065946.t1	MTR_8g066975	24.573	293	180	8	73	344	7	279	1.15e-13	71.2
MS.gene065946.t1	MTR_4g018750	24.060	266	189	8	74	335	15	271	1.45e-13	70.9
MS.gene065946.t1	MTR_2g102010	24.476	286	199	6	73	354	16	288	1.50e-13	70.9
MS.gene065946.t1	MTR_2g436930	29.082	196	112	4	180	354	39	228	3.17e-13	68.9
MS.gene065946.t1	MTR_7g109135	22.408	299	209	8	61	349	9	294	8.21e-13	68.9
MS.gene065946.t1	MTR_8g090210	25.000	308	184	12	77	360	113	397	9.99e-13	69.3
MS.gene065946.t1	MTR_4g057850	25.155	322	168	12	76	365	27	307	1.23e-12	68.6
MS.gene065946.t1	MTR_4g012850	25.692	253	174	5	119	364	51	296	1.53e-12	68.2
MS.gene065946.t1	MTR_4g012850	26.432	227	150	6	36	260	75	286	4.06e-11	63.5
MS.gene065946.t1	MTR_4g018750	24.303	251	177	8	74	320	15	256	9.23e-12	65.5
MS.gene065946.t1	MTR_5g032420	21.782	303	195	8	74	342	6	300	7.86e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene065946.t1	AT4G28390	80.159	378	65	2	3	370	2	379	0.0	583
MS.gene065946.t1	AT4G28390	80.159	378	65	2	3	370	2	379	0.0	583
MS.gene065946.t1	AT5G13490	74.286	385	82	4	1	369	1	384	0.0	544
MS.gene065946.t1	AT5G13490	74.286	385	82	4	1	369	1	384	0.0	544
MS.gene065946.t1	AT3G08580	74.278	381	85	4	1	369	1	380	0.0	527
MS.gene065946.t1	AT3G08580	74.278	381	85	4	1	369	1	380	0.0	527
MS.gene065946.t1	AT5G17400	61.644	292	112	0	70	361	10	301	3.71e-132	380
MS.gene065946.t1	AT5G56450	38.284	303	168	7	70	360	30	325	3.93e-62	202
MS.gene065946.t1	AT4G26180	33.225	307	175	9	70	360	17	309	1.22e-33	127
MS.gene065946.t1	AT4G26180	33.564	289	163	8	70	343	17	291	2.76e-32	123
MS.gene065946.t1	AT2G37890	29.641	334	216	8	37	360	8	332	3.16e-32	124
MS.gene065946.t1	AT3G55640	28.814	295	193	7	73	358	37	323	1.68e-30	119
MS.gene065946.t1	AT3G55640	28.814	295	193	7	73	358	37	323	1.68e-30	119
MS.gene065946.t1	AT1G14560	29.893	281	171	6	85	349	38	308	4.08e-30	118
MS.gene065946.t1	AT1G14560	30.182	275	166	6	85	343	38	302	2.59e-29	115
MS.gene065946.t1	AT3G51870	32.979	282	160	10	85	360	102	360	2.18e-28	114
MS.gene065946.t1	AT3G53940	29.870	308	195	9	64	360	63	360	4.24e-28	113
MS.gene065946.t1	AT4G32400	30.605	281	169	9	74	349	114	373	1.50e-27	112
MS.gene065946.t1	AT4G32400	26.020	196	125	5	75	267	208	386	3.84e-12	67.4
MS.gene065946.t1	AT5G01500	33.212	274	158	11	85	354	130	382	3.66e-27	111
MS.gene065946.t1	AT4G01100	27.974	311	186	10	77	360	45	344	1.05e-24	103
MS.gene065946.t1	AT5G51050	29.643	280	174	7	74	349	223	483	8.08e-24	102
MS.gene065946.t1	AT4G01100	27.385	325	184	11	77	360	45	358	2.20e-23	100
MS.gene065946.t1	AT4G01100	27.213	305	184	10	83	360	1	294	1.03e-22	97.4
MS.gene065946.t1	AT4G01100	25.854	205	129	6	77	266	98	294	6.65e-12	66.2
MS.gene065946.t1	AT5G61810	27.857	280	174	7	74	349	65	320	1.92e-22	97.1
MS.gene065946.t1	AT5G48970	27.673	318	188	8	71	354	19	328	2.30e-22	97.1
MS.gene065946.t1	AT3G21390	26.349	315	195	7	71	354	16	324	4.08e-22	96.3
MS.gene065946.t1	AT3G20240	25.085	295	185	6	73	349	51	327	4.46e-22	96.3
MS.gene065946.t1	AT3G21390	26.349	315	195	7	71	354	38	346	8.51e-22	95.5
MS.gene065946.t1	AT3G55640	28.718	195	127	3	73	263	37	223	1.06e-21	93.6
MS.gene065946.t1	AT3G55640	28.718	195	127	3	73	263	37	223	1.06e-21	93.6
MS.gene065946.t1	AT5G61810	27.857	280	174	7	74	349	208	463	2.03e-21	95.9
MS.gene065946.t1	AT3G53940	29.630	216	138	4	64	274	63	269	1.15e-19	88.6
MS.gene065946.t1	AT1G14560	31.383	188	112	4	178	349	24	210	1.33e-19	87.0
MS.gene065946.t1	AT5G07320	26.296	270	173	7	83	349	218	464	1.81e-19	90.1
MS.gene065946.t1	AT5G48970	26.953	256	152	6	126	354	10	257	2.63e-16	78.6
MS.gene065946.t1	AT5G64970	27.458	295	170	11	77	349	138	410	2.44e-15	77.4
MS.gene065946.t1	AT3G54110	23.485	264	193	7	74	335	15	271	5.59e-15	75.1
MS.gene065946.t1	AT1G14140	24.820	278	192	7	64	335	8	274	7.20e-15	75.1
MS.gene065946.t1	AT5G64970	26.780	295	172	11	77	349	14	286	1.12e-14	74.3
MS.gene065946.t1	AT5G64970	27.778	288	164	11	77	342	138	403	2.12e-14	74.3
MS.gene065946.t1	AT5G58970	24.460	278	195	6	70	342	12	279	2.60e-14	73.2
MS.gene065946.t1	AT5G58970	27.919	197	123	7	175	357	12	203	7.51e-11	63.2
MS.gene065946.t1	AT1G78180	27.273	308	177	12	77	360	131	415	2.25e-13	71.2
MS.gene065946.t1	AT5G66380	27.805	205	124	6	172	364	5	197	1.79e-12	67.8
MS.gene065946.t1	AT4G27940	25.368	272	168	12	114	360	146	407	2.44e-11	65.1
MS.gene065946.t1	AT5G46800	24.830	294	202	8	73	354	7	293	2.60e-11	64.3
MS.gene065946.t1	AT5G46800	24.830	294	202	8	73	354	7	293	2.60e-11	64.3
MS.gene065946.t1	AT5G46800	24.830	294	202	8	73	354	7	293	2.60e-11	64.3
MS.gene065946.t1	AT5G58970	27.919	197	123	7	175	357	12	203	5.84e-11	63.2

Find 101 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCCTATTTGTCTATTCTT+TGG	0.187425	4.2:-31398072	MS.gene065946:CDS
GATAGGTGGACATTCTTATT+TGG	0.215860	4.2:-31399081	MS.gene065946:CDS
TGAGCATTTCATCCTGGTTT+TGG	0.229183	4.2:+31398826	None:intergenic
GACATTCTTATTTGGTTTCC+AGG	0.255723	4.2:-31399073	MS.gene065946:CDS
GTCTTGTTTGGAAAGAAATA+TGG	0.260053	4.2:-31397088	MS.gene065946:CDS
GGTGGTGAGAGGCAGTTTAA+TGG	0.306537	4.2:-31398002	MS.gene065946:CDS
GCAGGTGCTGGTGTGCTTGC+TGG	0.309350	4.2:-31397130	MS.gene065946:CDS
TATCACAATTGGTGCTGGTT+TGG	0.315135	4.2:-31397311	MS.gene065946:CDS
AATCCAAAGAATAGACAAAT+AGG	0.321819	4.2:+31398069	None:intergenic
CAGTCACCAATTCCCTTATA+TGG	0.327603	4.2:+31398783	None:intergenic
TGCTAGTTTCCTGTTGGGAT+GGG	0.328388	4.2:-31397335	MS.gene065946:CDS
AATTATACCCTCATCCTTCA+TGG	0.333517	4.2:+31398749	None:intergenic
TTAGTATCTGGTGGAGCTGC+TGG	0.333758	4.2:-31398104	MS.gene065946:CDS
AACATATTGCGTGCTGTTGC+AGG	0.340667	4.2:-31397148	MS.gene065946:CDS
TTCTTTGCTAGTTTCCTGTT+GGG	0.343196	4.2:-31397340	MS.gene065946:CDS
CCTATCTTCTGAGCAATTGA+TGG	0.352718	4.2:+31399098	None:intergenic
AGAAGAATGATGATGACTTC+TGG	0.353166	4.2:-31397262	MS.gene065946:CDS
AAGCTGCAGCTGGTCTTGTT+TGG	0.353609	4.2:-31397100	MS.gene065946:CDS
TTACAGTCTGATGGCATTGC+TGG	0.359420	4.2:-31397954	MS.gene065946:CDS
AGCAATTTAATTCGTTCAAT+AGG	0.374655	4.2:+31398852	None:intergenic
GGCTACTGGAAGTGGTTTGC+TGG	0.374984	4.2:-31398131	MS.gene065946:CDS
TTTGGAAAGAAATATGGTTC+TGG	0.380189	4.2:-31397082	MS.gene065946:CDS
CTTTGCGTCATTTGCCAAAC+GGG	0.396020	4.2:+31398037	None:intergenic
ACTAATGGAGTGCAGACTTC+TGG	0.400993	4.2:-31399002	MS.gene065946:CDS
TTGCTAGTTTCCTGTTGGGA+TGG	0.404433	4.2:-31397336	MS.gene065946:CDS
AGATGTTGAATCCACGATAA+AGG	0.405874	4.2:+31397931	None:intergenic
CCATCAATTGCTCAGAAGAT+AGG	0.406150	4.2:-31399098	MS.gene065946:CDS
GGATTATGCCCGTACCCGTT+TGG	0.408041	4.2:-31398051	MS.gene065946:CDS
CTGAAACCAGTTGTCTTGGT+TGG	0.408866	4.2:-31397855	MS.gene065946:CDS
TCTGAGCCATATAAGGGAAT+TGG	0.421286	4.2:-31398789	MS.gene065946:CDS
TCTTCTACGCACAGTGTCAA+TGG	0.421926	4.2:+31397279	None:intergenic
GTTAGAGTTGGCGCTAAGCC+TGG	0.422723	4.2:+31399055	None:intergenic
CAGGTTATCTGAGCCATATA+AGG	0.423186	4.2:-31398796	MS.gene065946:CDS
TAGTATCTGGTGGAGCTGCT+GGG	0.429291	4.2:-31398103	MS.gene065946:CDS
GTGTATCGTGGTCTTTACTT+TGG	0.436559	4.2:-31397891	MS.gene065946:CDS
TGGGGTATCACAATTGGTGC+TGG	0.437821	4.2:-31397316	MS.gene065946:CDS
TTTGCTGGGAATTTAGTATC+TGG	0.438983	4.2:-31398116	MS.gene065946:CDS
CCAAACCATCGTTGCAAAAG+AGG	0.440517	4.2:-31397203	MS.gene065946:CDS
TGGAGTGCAGACTTCTGGTT+TGG	0.442748	4.2:-31398997	MS.gene065946:CDS
TGACGCAAAGGCTGCAAAGA+AGG	0.445560	4.2:-31398024	MS.gene065946:CDS
TTGGGATGGGGTATCACAAT+TGG	0.445710	4.2:-31397322	MS.gene065946:CDS
TTTCTTTGCTAGTTTCCTGT+TGG	0.448910	4.2:-31397341	MS.gene065946:CDS
CGTCATTTGCCAAACGGGTA+CGG	0.451569	4.2:+31398042	None:intergenic
TTCTCTGAAACCAGTTGTCT+TGG	0.454364	4.2:-31397859	MS.gene065946:CDS
ACGTCCGGTACTTTCACTAA+TGG	0.459227	4.2:-31399017	MS.gene065946:CDS
CCAGCGGGTCCTTTCTCTGC+TGG	0.461085	4.2:+31398936	None:intergenic
TTGCGTGCTGTTGCAGGTGC+TGG	0.462273	4.2:-31397142	MS.gene065946:CDS
AAGAAATATGGTTCTGGCGG+CGG	0.463431	4.2:-31397076	MS.gene065946:CDS
GGTCCTTTCTCTGCTGGAGA+AGG	0.466249	4.2:+31398942	None:intergenic
AAACCAGTTGTCTTGGTTGG+TGG	0.469067	4.2:-31397852	MS.gene065946:CDS
TTGTGATACCCCATCCCAAC+AGG	0.471309	4.2:+31397326	None:intergenic
CACAGTGTCAATGGGATAAG+AGG	0.473276	4.2:+31397288	None:intergenic
ATTCAGATTATCACCTGAGT+AGG	0.479406	4.2:+31398684	None:intergenic
GCTAGTTTCCTGTTGGGATG+GGG	0.479609	4.2:-31397334	MS.gene065946:CDS
TGTCTTGGTTGGTGGGATGC+AGG	0.479765	4.2:-31397844	MS.gene065946:intron
ATCCCACCAACCAAGACAAC+TGG	0.485200	4.2:+31397849	None:intergenic
AGGTTATCTGAGCCATATAA+GGG	0.488890	4.2:-31398795	MS.gene065946:CDS
CCTTTGCGTCATTTGCCAAA+CGG	0.490345	4.2:+31398036	None:intergenic
GTCATTTGCCAAACGGGTAC+GGG	0.492794	4.2:+31398043	None:intergenic
ACAGAGATGGCTACTGGAAG+TGG	0.495428	4.2:-31398139	MS.gene065946:CDS
GCTGGGAATTTAGTATCTGG+TGG	0.499128	4.2:-31398113	MS.gene065946:CDS
AGCAATGCCATCAGACTGTA+AGG	0.504971	4.2:+31397956	None:intergenic
TACAGGAATTACTCTACGTC+CGG	0.508953	4.2:-31399032	MS.gene065946:CDS
GTCCCTTCTCCAGCAGAGAA+AGG	0.510593	4.2:-31398945	MS.gene065946:CDS
CTTCTACGCACAGTGTCAAT+GGG	0.512712	4.2:+31397280	None:intergenic
GTAATTCCTGTAGTTAGAGT+TGG	0.521037	4.2:+31399043	None:intergenic
AACCAGTTGTCTTGGTTGGT+GGG	0.523148	4.2:-31397851	MS.gene065946:CDS
TGCTCGAACCATGAAGGATG+AGG	0.524575	4.2:-31398757	MS.gene065946:CDS
TGGTTATGACAAGCTGCAGC+TGG	0.524956	4.2:-31397110	MS.gene065946:CDS
GGCATTGCTGGCCTTTATCG+TGG	0.542365	4.2:-31397942	MS.gene065946:CDS
GTCCTTTCTCTGCTGGAGAA+GGG	0.549707	4.2:+31398943	None:intergenic
GCTACTGGAAGTGGTTTGCT+GGG	0.556481	4.2:-31398130	MS.gene065946:CDS
GGATTCAACATCTCGTGTGT+TGG	0.556905	4.2:-31397921	MS.gene065946:CDS
ATGAAATGCTCAAAAGTGGC+AGG	0.556927	4.2:-31398815	MS.gene065946:CDS
AGTATCTGGTGGAGCTGCTG+GGG	0.568550	4.2:-31398102	MS.gene065946:CDS
TTAGCGCCAACTCTAACTAC+AGG	0.570302	4.2:-31399049	MS.gene065946:CDS
TTCAGATTATCACCTGAGTA+GGG	0.571644	4.2:+31398685	None:intergenic
GACGCAAAGGCTGCAAAGAA+GGG	0.572621	4.2:-31398023	MS.gene065946:CDS
CCAGCAGAGAAAGGACCCGC+TGG	0.578869	4.2:-31398936	MS.gene065946:CDS
TGAGAGGCAGTTTAATGGAA+TGG	0.581022	4.2:-31397997	MS.gene065946:CDS
CAAACCATCGTTGCAAAAGA+GGG	0.586013	4.2:-31397202	MS.gene065946:CDS
CAGGATGAAATGCTCAAAAG+TGG	0.586952	4.2:-31398819	MS.gene065946:CDS
AAGAAAACCTTACAGTCTGA+TGG	0.592392	4.2:-31397963	MS.gene065946:CDS
TAGGCTGCAATCATGGCCGA+TGG	0.596371	4.2:-31399131	None:intergenic
CACTCCATTAGTGAAAGTAC+CGG	0.601548	4.2:+31399013	None:intergenic
TAAATTGCTTATCCAAAACC+AGG	0.601587	4.2:-31398838	MS.gene065946:CDS
GGTATAATTGCTCTGTGGAG+AGG	0.606231	4.2:-31398735	MS.gene065946:CDS
GCTCGAACCATGAAGGATGA+GGG	0.608674	4.2:-31398756	MS.gene065946:CDS
TGATGGATGATGTGGTCCAT+CGG	0.611029	4.2:+31399115	None:intergenic
GGAAAGAAATATGGTTCTGG+CGG	0.618520	4.2:-31397079	MS.gene065946:CDS
TCAATTGCTCAGAAGATAGG+TGG	0.621358	4.2:-31399095	MS.gene065946:CDS
CTGCAAAGAAGGGTGGTGAG+AGG	0.621915	4.2:-31398013	MS.gene065946:CDS
AGAATAGACAAATAGGAGTG+AGG	0.622653	4.2:+31398076	None:intergenic
CCGTTTGGCAAATGACGCAA+AGG	0.622778	4.2:-31398036	MS.gene065946:CDS
TATCAGATACTTCCCTACTC+AGG	0.622842	4.2:-31398697	MS.gene065946:intron
TGAGCAATTGATGGATGATG+TGG	0.652110	4.2:+31399107	None:intergenic
ATAGACAAATAGGAGTGAGG+AGG	0.664089	4.2:+31398079	None:intergenic
GTTGGAATTATAGTGTATCG+TGG	0.683295	4.2:-31397903	MS.gene065946:CDS
GCAAAGGCTGCAAAGAAGGG+TGG	0.689413	4.2:-31398020	MS.gene065946:CDS
ATGAGGGTATAATTGCTCTG+TGG	0.733403	4.2:-31398740	MS.gene065946:CDS
GGGACAGTAAATGACGACAG+AGG	0.743353	4.2:+31398963	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGAATGAAATTAGAAATTAA+AGG	+	chr4.2:31397966-31397985	None:intergenic	15.0%
!!	ATGAACATTACTAAAATAAT+TGG	+	chr4.2:31398787-31398806	None:intergenic	15.0%
!!	TGAACATTACTAAAATAATT+GGG	+	chr4.2:31398786-31398805	None:intergenic	15.0%
!!!	ATGTACAAATTTGATTTTAA+CGG	-	chr4.2:31398371-31398390	MS.gene065946:intron	15.0%
!!	GAACATTACTAAAATAATTG+GGG	+	chr4.2:31398785-31398804	None:intergenic	20.0%
!!	TTGAAACTTTAATTGAACTA+GGG	-	chr4.2:31398724-31398743	MS.gene065946:CDS	20.0%
!!!	AATTTCATTCTTTTCCTTTT+AGG	-	chr4.2:31397975-31397994	MS.gene065946:CDS	20.0%
!!!	AATTTTAAAAAAGACAGAGA+TGG	-	chr4.2:31398039-31398058	MS.gene065946:CDS	20.0%
!!!	ATTGAGATTAGCTTATATTT+TGG	-	chr4.2:31397786-31397805	MS.gene065946:intron	20.0%
!!!	CATCATTTTCTTATTATCTA+TGG	-	chr4.2:31398804-31398823	MS.gene065946:CDS	20.0%
!!!	GGAATAATTTTAATCTAACA+CGG	+	chr4.2:31398422-31398441	None:intergenic	20.0%
!!!	TAAAATTATTCCTCTTTTGT+TGG	-	chr4.2:31398430-31398449	MS.gene065946:intron	20.0%
!!!	TGTACAAATTTGATTTTAAC+GGG	-	chr4.2:31398372-31398391	MS.gene065946:intron	20.0%
!!!	TTTTAATAGAAAGATGTCTA+GGG	+	chr4.2:31398669-31398688	None:intergenic	20.0%
!	AAATGCAAGGATACAAAAAT+AGG	+	chr4.2:31397835-31397854	None:intergenic	25.0%
!	AATCCAAAGAATAGACAAAT+AGG	+	chr4.2:31398125-31398144	None:intergenic	25.0%
!	AGAAATTTGTGGAAATCTAA+TGG	-	chr4.2:31397633-31397652	MS.gene065946:intron	25.0%
!	AGCAATTTAATTCGTTCAAT+AGG	+	chr4.2:31397342-31397361	None:intergenic	25.0%
!	ATAAGTTATATGCGTTGTAA+GGG	-	chr4.2:31397578-31397597	MS.gene065946:intron	25.0%
!	CTAAAAGAAAGTCAAAATTG+TGG	+	chr4.2:31398762-31398781	None:intergenic	25.0%
!	CTTGAAACTTTAATTGAACT+AGG	-	chr4.2:31398723-31398742	MS.gene065946:CDS	25.0%
!	TTAGATTTCCACAAATTTCT+TGG	+	chr4.2:31397633-31397652	None:intergenic	25.0%
!!	AGAAAATGTTTTGTATGTCT+AGG	+	chr4.2:31397863-31397882	None:intergenic	25.0%
!!	GAAAATGTTTTGTATGTCTA+GGG	+	chr4.2:31397862-31397881	None:intergenic	25.0%
!!!	ACTTTGATTTTATGTTTGTC+AGG	-	chr4.2:31398457-31398476	MS.gene065946:intron	25.0%
!!!	AGATTAGCTTATATTTTGGA+AGG	-	chr4.2:31397790-31397809	MS.gene065946:intron	25.0%
!!!	ATTTTTGATCGATTTTCTGA+TGG	-	chr4.2:31397278-31397297	MS.gene065946:CDS	25.0%
!!!	GATTAGCTTATATTTTGGAA+GGG	-	chr4.2:31397791-31397810	MS.gene065946:intron	25.0%
!!!	GTAAAAGGTGTTTAATAGAA+AGG	+	chr4.2:31398647-31398666	None:intergenic	25.0%
!!!	GTTTTAATAGAAAGATGTCT+AGG	+	chr4.2:31398670-31398689	None:intergenic	25.0%
!!!	TTTGATTTTAACGGGAAATA+TGG	-	chr4.2:31398380-31398399	MS.gene065946:intron	25.0%
!!!	TTTTTGATCGATTTTCTGAT+GGG	-	chr4.2:31397279-31397298	MS.gene065946:CDS	25.0%
	AAAATCGATCAAAAATCCAG+CGG	+	chr4.2:31397274-31397293	None:intergenic	30.0%
	ACTCCTATTTGTCTATTCTT+TGG	-	chr4.2:31398119-31398138	MS.gene065946:CDS	30.0%
	CAAAGTAAAACCAACAAAAG+AGG	+	chr4.2:31398443-31398462	None:intergenic	30.0%
	CATAAGTTATATGCGTTGTA+AGG	-	chr4.2:31397577-31397596	MS.gene065946:intron	30.0%
	CTTACAACGCATATAACTTA+TGG	+	chr4.2:31397579-31397598	None:intergenic	30.0%
	GTGTTAAACCAAGAAATTTG+TGG	-	chr4.2:31397622-31397641	MS.gene065946:intron	30.0%
	TAAATTGCTTATCCAAAACC+AGG	-	chr4.2:31397353-31397372	MS.gene065946:intron	30.0%
	TTAACACAATATACACTTCG+AGG	+	chr4.2:31397609-31397628	None:intergenic	30.0%
	TTTAGCAATCTATGATGTCT+AGG	+	chr4.2:31398703-31398722	None:intergenic	30.0%
	TTTGGAAAGAAATATGGTTC+TGG	-	chr4.2:31399109-31399128	MS.gene065946:CDS	30.0%
!	ATATACACATTACTGATTGC+AGG	+	chr4.2:31398619-31398638	None:intergenic	30.0%
!	ATGGTACTTTTCTTGTTAAC+AGG	-	chr4.2:31398823-31398842	MS.gene065946:CDS	30.0%
!	CTATGATGTCTAGGATATAA+AGG	+	chr4.2:31398694-31398713	None:intergenic	30.0%
!!	CTTTTAAGGATTACTTCAAG+AGG	-	chr4.2:31398010-31398029	MS.gene065946:CDS	30.0%
!!	GTCTTGTTTGGAAAGAAATA+TGG	-	chr4.2:31399103-31399122	MS.gene065946:CDS	30.0%
	AAAAACTGTCACTGTGATTC+CGG	-	chr4.2:31397701-31397720	MS.gene065946:intron	35.0%
	AAATCGATCAAAAATCCAGC+GGG	+	chr4.2:31397273-31397292	None:intergenic	35.0%
	AAGAAAACCTTACAGTCTGA+TGG	-	chr4.2:31398228-31398247	MS.gene065946:intron	35.0%
	AATTATACCCTCATCCTTCA+TGG	+	chr4.2:31397445-31397464	None:intergenic	35.0%
	AGAATAGACAAATAGGAGTG+AGG	+	chr4.2:31398118-31398137	None:intergenic	35.0%
	AGATGTTGAATCCACGATAA+AGG	+	chr4.2:31398263-31398282	None:intergenic	35.0%
	AGGTTATCTGAGCCATATAA+GGG	-	chr4.2:31397396-31397415	MS.gene065946:intron	35.0%
	ATTCAGATTATCACCTGAGT+AGG	+	chr4.2:31397510-31397529	None:intergenic	35.0%
	GATAGGTGGACATTCTTATT+TGG	-	chr4.2:31397110-31397129	MS.gene065946:CDS	35.0%
	GTAATTCCTGTAGTTAGAGT+TGG	+	chr4.2:31397151-31397170	None:intergenic	35.0%
	GTTGGAATTATAGTGTATCG+TGG	-	chr4.2:31398288-31398307	MS.gene065946:intron	35.0%
	TGTGGTGTTTGTATAAGTAG+AGG	+	chr4.2:31397678-31397697	None:intergenic	35.0%
	TTCAGATTATCACCTGAGTA+GGG	+	chr4.2:31397509-31397528	None:intergenic	35.0%
	TTTGCTGGGAATTTAGTATC+TGG	-	chr4.2:31398075-31398094	MS.gene065946:CDS	35.0%
!	ACAGTGACAGTTTTTGTATG+TGG	+	chr4.2:31397696-31397715	None:intergenic	35.0%
!	AGAAAGATGTCTAGGGTAAA+AGG	+	chr4.2:31398662-31398681	None:intergenic	35.0%
!	AGAAGAATGATGATGACTTC+TGG	-	chr4.2:31398929-31398948	MS.gene065946:CDS	35.0%
!	GACATTCTTATTTGGTTTCC+AGG	-	chr4.2:31397118-31397137	MS.gene065946:CDS	35.0%
!	GGTACTAAGTCTCTTTTCAA+AGG	-	chr4.2:31399010-31399029	MS.gene065946:CDS	35.0%
!	TTCTTTGCTAGTTTCCTGTT+GGG	-	chr4.2:31398851-31398870	MS.gene065946:CDS	35.0%
!	TTTCCATGTGTTGTCTTGTT+TGG	-	chr4.2:31397735-31397754	MS.gene065946:intron	35.0%
!	TTTCTTTGCTAGTTTCCTGT+TGG	-	chr4.2:31398850-31398869	MS.gene065946:CDS	35.0%
!!	CTCTTTTGGTGATTGAATGT+TGG	-	chr4.2:31397917-31397936	MS.gene065946:CDS	35.0%
!!	TTCCATGTGTTGTCTTGTTT+GGG	-	chr4.2:31397736-31397755	MS.gene065946:intron	35.0%
	AAAAAGACAGAGATGGCTAC+TGG	-	chr4.2:31398046-31398065	MS.gene065946:CDS	40.0%
	AACCCAAACAAGACAACACA+TGG	+	chr4.2:31397741-31397760	None:intergenic	40.0%
	AGCAAAGTTCAGAGCCTAAA+AGG	+	chr4.2:31397992-31398011	None:intergenic	40.0%
	ATAGACAAATAGGAGTGAGG+AGG	+	chr4.2:31398115-31398134	None:intergenic	40.0%
	ATGAAATGCTCAAAAGTGGC+AGG	-	chr4.2:31397376-31397395	MS.gene065946:intron	40.0%
	CAAACCATCGTTGCAAAAGA+GGG	-	chr4.2:31398989-31399008	MS.gene065946:CDS	40.0%
	CAGGATGAAATGCTCAAAAG+TGG	-	chr4.2:31397372-31397391	MS.gene065946:intron	40.0%
	CAGGTTATCTGAGCCATATA+AGG	-	chr4.2:31397395-31397414	MS.gene065946:intron	40.0%
	CAGTCACCAATTCCCTTATA+TGG	+	chr4.2:31397411-31397430	None:intergenic	40.0%
	CATGGAAAATCTCATGACAC+CGG	+	chr4.2:31397723-31397742	None:intergenic	40.0%
	CCATCAATTGCTCAGAAGAT+AGG	-	chr4.2:31397093-31397112	MS.gene065946:CDS	40.0%
	CCTATCTTCTGAGCAATTGA+TGG	+	chr4.2:31397096-31397115	None:intergenic	40.0%
	GTGTATCGTGGTCTTTACTT+TGG	-	chr4.2:31398300-31398319	MS.gene065946:intron	40.0%
	TACAGGAATTACTCTACGTC+CGG	-	chr4.2:31397159-31397178	MS.gene065946:CDS	40.0%
	TATCAGATACTTCCCTACTC+AGG	-	chr4.2:31397494-31397513	MS.gene065946:intron	40.0%
	TCAATTGCTCAGAAGATAGG+TGG	-	chr4.2:31397096-31397115	MS.gene065946:CDS	40.0%
	TCTGAGCCATATAAGGGAAT+TGG	-	chr4.2:31397402-31397421	MS.gene065946:intron	40.0%
	TGAGAGGCAGTTTAATGGAA+TGG	-	chr4.2:31398194-31398213	MS.gene065946:CDS	40.0%
	TGTCTAGGGATGTAAATGCA+AGG	+	chr4.2:31397848-31397867	None:intergenic	40.0%
!	ATGAGGGTATAATTGCTCTG+TGG	-	chr4.2:31397451-31397470	MS.gene065946:intron	40.0%
!	CACTCCATTAGTGAAAGTAC+CGG	+	chr4.2:31397181-31397200	None:intergenic	40.0%
!	CACTTTTGAGCATTTCATCC+TGG	+	chr4.2:31397374-31397393	None:intergenic	40.0%
!	GATGCATGTGAATGCTCTTT+TGG	-	chr4.2:31397903-31397922	MS.gene065946:CDS	40.0%
!	TGAGCAATTGATGGATGATG+TGG	+	chr4.2:31397087-31397106	None:intergenic	40.0%
!	TGAGCATTTCATCCTGGTTT+TGG	+	chr4.2:31397368-31397387	None:intergenic	40.0%
!!	AAGTCTCTTTTCAAAGGTGC+TGG	-	chr4.2:31399016-31399035	MS.gene065946:CDS	40.0%
!!	GGAAAGAAATATGGTTCTGG+CGG	-	chr4.2:31399112-31399131	MS.gene065946:CDS	40.0%
!!	TATCACAATTGGTGCTGGTT+TGG	-	chr4.2:31398880-31398899	MS.gene065946:CDS	40.0%
!!	TTCTCTGAAACCAGTTGTCT+TGG	-	chr4.2:31398332-31398351	MS.gene065946:intron	40.0%
!!	TTGATCGATTTTCTGATGGG+AGG	-	chr4.2:31397282-31397301	MS.gene065946:CDS	40.0%
!!!	GGCTCTGAACTTTGCTTTTA+AGG	-	chr4.2:31397996-31398015	MS.gene065946:CDS	40.0%
	AAACCAGTTGTCTTGGTTGG+TGG	-	chr4.2:31398339-31398358	MS.gene065946:intron	45.0%
	AACCAGTTGTCTTGGTTGGT+GGG	-	chr4.2:31398340-31398359	MS.gene065946:intron	45.0%
	ACGTCCGGTACTTTCACTAA+TGG	-	chr4.2:31397174-31397193	MS.gene065946:CDS	45.0%
	ACTAATGGAGTGCAGACTTC+TGG	-	chr4.2:31397189-31397208	MS.gene065946:CDS	45.0%
	AGCAATGCCATCAGACTGTA+AGG	+	chr4.2:31398238-31398257	None:intergenic	45.0%
	CACAGTGTCAATGGGATAAG+AGG	+	chr4.2:31398906-31398925	None:intergenic	45.0%
	CCAAACCATCGTTGCAAAAG+AGG	-	chr4.2:31398988-31399007	MS.gene065946:CDS	45.0%
	CCTTTGCGTCATTTGCCAAA+CGG	+	chr4.2:31398158-31398177	None:intergenic	45.0%
	CTGAAACCAGTTGTCTTGGT+TGG	-	chr4.2:31398336-31398355	MS.gene065946:intron	45.0%
	CTTCTACGCACAGTGTCAAT+GGG	+	chr4.2:31398914-31398933	None:intergenic	45.0%
	CTTTGCGTCATTTGCCAAAC+GGG	+	chr4.2:31398157-31398176	None:intergenic	45.0%
	GCTGGGAATTTAGTATCTGG+TGG	-	chr4.2:31398078-31398097	MS.gene065946:CDS	45.0%
	GGATTCAACATCTCGTGTGT+TGG	-	chr4.2:31398270-31398289	MS.gene065946:intron	45.0%
	GGTATAATTGCTCTGTGGAG+AGG	-	chr4.2:31397456-31397475	MS.gene065946:intron	45.0%
	TCTTCTACGCACAGTGTCAA+TGG	+	chr4.2:31398915-31398934	None:intergenic	45.0%
	TTACAGTCTGATGGCATTGC+TGG	-	chr4.2:31398237-31398256	MS.gene065946:intron	45.0%
	TTAGCGCCAACTCTAACTAC+AGG	-	chr4.2:31397142-31397161	MS.gene065946:CDS	45.0%
!	AACATATTGCGTGCTGTTGC+AGG	-	chr4.2:31399043-31399062	MS.gene065946:CDS	45.0%
!	AGTACCCTCTTTTGCAACGA+TGG	+	chr4.2:31398996-31399015	None:intergenic	45.0%
!	CCTCTTTTGCAACGATGGTT+TGG	+	chr4.2:31398991-31399010	None:intergenic	45.0%
!	CTGTTTTGCTCGAACCATGA+AGG	-	chr4.2:31397428-31397447	MS.gene065946:intron	45.0%
!	TGATGGATGATGTGGTCCAT+CGG	+	chr4.2:31397079-31397098	None:intergenic	45.0%
!	TGCTAGTTTCCTGTTGGGAT+GGG	-	chr4.2:31398856-31398875	MS.gene065946:CDS	45.0%
!	TTGCTAGTTTCCTGTTGGGA+TGG	-	chr4.2:31398855-31398874	MS.gene065946:CDS	45.0%
!	TTGGGATGGGGTATCACAAT+TGG	-	chr4.2:31398869-31398888	MS.gene065946:CDS	45.0%
!!	AAGAAATATGGTTCTGGCGG+CGG	-	chr4.2:31399115-31399134	MS.gene065946:CDS	45.0%
	ACAGAGATGGCTACTGGAAG+TGG	-	chr4.2:31398052-31398071	MS.gene065946:CDS	50.0%
	ATCCCACCAACCAAGACAAC+TGG	+	chr4.2:31398345-31398364	None:intergenic	50.0%
	CCGTTTGGCAAATGACGCAA+AGG	-	chr4.2:31398155-31398174	MS.gene065946:CDS	50.0%
	CGTCATTTGCCAAACGGGTA+CGG	+	chr4.2:31398152-31398171	None:intergenic	50.0%
	GACGCAAAGGCTGCAAAGAA+GGG	-	chr4.2:31398168-31398187	MS.gene065946:CDS	50.0%
	GCTCGAACCATGAAGGATGA+GGG	-	chr4.2:31397435-31397454	MS.gene065946:intron	50.0%
	GGGACAGTAAATGACGACAG+AGG	+	chr4.2:31397231-31397250	None:intergenic	50.0%
	GGTGGTGAGAGGCAGTTTAA+TGG	-	chr4.2:31398189-31398208	MS.gene065946:CDS	50.0%
	GTCATTTGCCAAACGGGTAC+GGG	+	chr4.2:31398151-31398170	None:intergenic	50.0%
	GTCCTTTCTCTGCTGGAGAA+GGG	+	chr4.2:31397251-31397270	None:intergenic	50.0%
	TAGTATCTGGTGGAGCTGCT+GGG	-	chr4.2:31398088-31398107	MS.gene065946:CDS	50.0%
	TGACGCAAAGGCTGCAAAGA+AGG	-	chr4.2:31398167-31398186	MS.gene065946:CDS	50.0%
	TGCTCGAACCATGAAGGATG+AGG	-	chr4.2:31397434-31397453	MS.gene065946:intron	50.0%
	TGGTTATGACAAGCTGCAGC+TGG	-	chr4.2:31399081-31399100	MS.gene065946:CDS	50.0%
	TTAGTATCTGGTGGAGCTGC+TGG	-	chr4.2:31398087-31398106	MS.gene065946:CDS	50.0%
	TTGTGATACCCCATCCCAAC+AGG	+	chr4.2:31398868-31398887	None:intergenic	50.0%
!	GCTAGTTTCCTGTTGGGATG+GGG	-	chr4.2:31398857-31398876	MS.gene065946:CDS	50.0%
!	TGGAGTGCAGACTTCTGGTT+TGG	-	chr4.2:31397194-31397213	MS.gene065946:CDS	50.0%
!	TGGGGTATCACAATTGGTGC+TGG	-	chr4.2:31398875-31398894	MS.gene065946:CDS	50.0%
!!	AAGCTGCAGCTGGTCTTGTT+TGG	-	chr4.2:31399091-31399110	MS.gene065946:CDS	50.0%
!!	GCTACTGGAAGTGGTTTGCT+GGG	-	chr4.2:31398061-31398080	MS.gene065946:CDS	50.0%
	AGTATCTGGTGGAGCTGCTG+GGG	-	chr4.2:31398089-31398108	MS.gene065946:CDS	55.0%
	CTGCAAAGAAGGGTGGTGAG+AGG	-	chr4.2:31398178-31398197	MS.gene065946:CDS	55.0%
	GCAAAGGCTGCAAAGAAGGG+TGG	-	chr4.2:31398171-31398190	MS.gene065946:CDS	55.0%
	GGATTATGCCCGTACCCGTT+TGG	-	chr4.2:31398140-31398159	MS.gene065946:CDS	55.0%
	GGTCCTTTCTCTGCTGGAGA+AGG	+	chr4.2:31397252-31397271	None:intergenic	55.0%
	GTCCCTTCTCCAGCAGAGAA+AGG	-	chr4.2:31397246-31397265	MS.gene065946:CDS	55.0%
	GTTAGAGTTGGCGCTAAGCC+TGG	+	chr4.2:31397139-31397158	None:intergenic	55.0%
!	GGCTACTGGAAGTGGTTTGC+TGG	-	chr4.2:31398060-31398079	MS.gene065946:CDS	55.0%
!!	GGCATTGCTGGCCTTTATCG+TGG	-	chr4.2:31398249-31398268	MS.gene065946:intron	55.0%
!!	TGTCTTGGTTGGTGGGATGC+AGG	-	chr4.2:31398347-31398366	MS.gene065946:intron	55.0%
!	TTGCGTGCTGTTGCAGGTGC+TGG	-	chr4.2:31399049-31399068	MS.gene065946:CDS	60.0%
	CCAGCAGAGAAAGGACCCGC+TGG	-	chr4.2:31397255-31397274	MS.gene065946:CDS	65.0%
	CCAGCGGGTCCTTTCTCTGC+TGG	+	chr4.2:31397258-31397277	None:intergenic	65.0%
!!	GCAGGTGCTGGTGTGCTTGC+TGG	-	chr4.2:31399061-31399080	MS.gene065946:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	31397072	31399141	31397072	ID=MS.gene065946
chr4.2	mRNA	31397072	31399141	31397072	ID=MS.gene065946.t1;Parent=MS.gene065946
chr4.2	exon	31398698	31399141	31398698	ID=MS.gene065946.t1.exon1;Parent=MS.gene065946.t1
chr4.2	CDS	31398698	31399141	31398698	ID=cds.MS.gene065946.t1;Parent=MS.gene065946.t1
chr4.2	exon	31397845	31398216	31397845	ID=MS.gene065946.t1.exon2;Parent=MS.gene065946.t1
chr4.2	CDS	31397845	31398216	31397845	ID=cds.MS.gene065946.t1;Parent=MS.gene065946.t1
chr4.2	exon	31397072	31397368	31397072	ID=MS.gene065946.t1.exon3;Parent=MS.gene065946.t1
chr4.2	CDS	31397072	31397368	31397072	ID=cds.MS.gene065946.t1;Parent=MS.gene065946.t1

Gene Sequence

>MS.gene065946

ATGGCCGATGGACCACATCATCCATCAATTGCTCAGAAGATAGGTGGACATTCTTATTTGGTTTCCAGGCTTAGCGCCAACTCTAACTACAGGAATTACTCTACGTCCGGTACTTTCACTAATGGAGTGCAGACTTCTGGTTTGGCACTTGCGTCACCTCTGTCGTCATTTACTGTCCCTTCTCCAGCAGAGAAAGGACCCGCTGGATTTTTGATCGATTTTCTGATGGGAGGAGTGTCTGCTGCTGTGTCAAAGACAGCTGCTGCTCCTATTGAACGAATTAAATTGCTTATCCAAAACCAGGATGAAATGCTCAAAAGTGGCAGGTTATCTGAGCCATATAAGGGAATTGGTGACTGTTTTGCTCGAACCATGAAGGATGAGGGTATAATTGCTCTGTGGAGAGGCAATACTGCTAATGTTATCAGATACTTCCCTACTCAGGTGATAATCTGAATTTTTTTCTTGTAAGATATTTTCTATTTTGACATATTCTTACAGCATCCATAAGTTATATGCGTTGTAAGGGTTGTTCCTCGAAGTGTATATTGTGTTAAACCAAGAAATTTGTGGAAATCTAATGGTTGAATATTCTTTTACTGACCTCTACTTATACAAACACCACATACAAAAACTGTCACTGTGATTCCGGTGTCATGAGATTTTCCATGTGTTGTCTTGTTTGGGTTTTGTTCATGCTGTCTTATTATATATATTGAGATTAGCTTATATTTTGGAAGGGAAAAGATTTTATATAATACCTATTTTTGTATCCTTGCATTTACATCCCTAGACATACAAAACATTTTCTCTCTTTTTTTATCTGATTTTGATGCATGTGAATGCTCTTTTGGTGATTGAATGTTGGTTCTGTAGTTTCTGCGAGCTGTTCCTTTAATTTCTAATTTCATTCTTTTCCTTTTAGGCTCTGAACTTTGCTTTTAAGGATTACTTCAAGAGGCTTTTCAATTTTAAAAAAGACAGAGATGGCTACTGGAAGTGGTTTGCTGGGAATTTAGTATCTGGTGGAGCTGCTGGGGCCTCCTCACTCCTATTTGTCTATTCTTTGGATTATGCCCGTACCCGTTTGGCAAATGACGCAAAGGCTGCAAAGAAGGGTGGTGAGAGGCAGTTTAATGGAATGGTTGATGTTTACAAGAAAACCTTACAGTCTGATGGCATTGCTGGCCTTTATCGTGGATTCAACATCTCGTGTGTTGGAATTATAGTGTATCGTGGTCTTTACTTTGGAATGTATGATTCTCTGAAACCAGTTGTCTTGGTTGGTGGGATGCAGGTATGTACAAATTTGATTTTAACGGGAAATATGGTGTTTCTTAATCTGTTCCGTGTTAGATTAAAATTATTCCTCTTTTGTTGGTTTTACTTTGATTTTATGTTTGTCAGGCAATATTTTGTTGATTGTAAACGATTAGCTTGAATATTTAATTTTTTATGTCTTAAATATAATTTGAAAATGATGCTTTTAGAATGCTAATCTAGTTTATTAGCACATTATCTGTAAGCATGTTAGTATTCAGTTACCTGCAATCAGTAATGTGTATATATCTTCCTTTCTATTAAACACCTTTTACCCTAGACATCTTTCTATTAAAACACCTTTATATCCTAGACATCATAGATTGCTAAACTTGAAACTTTAATTGAACTAGGGAAATTTGTTCTTCCACAATTTTGACTTTCTTTTAGCCCCAATTATTTTAGTAATGTTCATCATTTTCTTATTATCTATGGTACTTTTCTTGTTAACAGGATAGTTTCTTTGCTAGTTTCCTGTTGGGATGGGGTATCACAATTGGTGCTGGTTTGGCCTCTTATCCCATTGACACTGTGCGTAGAAGAATGATGATGACTTCTGGAGAAGCTGTGAAGTATAACAGCTCTTTGCATGCATTCCAAACCATCGTTGCAAAAGAGGGTACTAAGTCTCTTTTCAAAGGTGCTGGTGCAAACATATTGCGTGCTGTTGCAGGTGCTGGTGTGCTTGCTGGTTATGACAAGCTGCAGCTGGTCTTGTTTGGAAAGAAATATGGTTCTGGCGGCGGCTAA

Protein sequence

>MS.gene065946.t1

MADGPHHPSIAQKIGGHSYLVSRLSANSNYRNYSTSGTFTNGVQTSGLALASPLSSFTVPSPAEKGPAGFLIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMLKSGRLSEPYKGIGDCFARTMKDEGIIALWRGNTANVIRYFPTQALNFAFKDYFKRLFNFKKDRDGYWKWFAGNLVSGGAAGASSLLFVYSLDYARTRLANDAKAAKKGGERQFNGMVDVYKKTLQSDGIAGLYRGFNISCVGIIVYRGLYFGMYDSLKPVVLVGGMQDSFFASFLLGWGITIGAGLASYPIDTVRRRMMMTSGEAVKYNSSLHAFQTIVAKEGTKSLFKGAGANILRAVAGAGVLAGYDKLQLVLFGKKYGSGGG