AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene49161


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49161.t1 MTR_1g099620 97.786 271 6 0 1 271 66 336 0.0 544
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene49161.t1 AT5G52810 66.279 258 85 1 11 268 66 321 9.06e-128 366

Find 67 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATAGAACCTCTTGTGTTTC+TGG 0.038368 1.3:-68664077 MS.gene49161:CDS
TTGGATGAGGTTGTTGGATT+TGG 0.201511 1.3:-68663828 MS.gene49161:CDS
TGGATGAGGTTGTTGGATTT+GGG 0.215659 1.3:-68663827 MS.gene49161:CDS
GTGATCAAGGAAGATGAAAT+TGG 0.263380 1.3:-68663597 MS.gene49161:CDS
CTCTCCTCCTCATGCCATCT+TGG 0.286730 1.3:-68664259 None:intergenic
TGGTCAAACCCTAGCTTCTA+TGG 0.293021 1.3:-68664120 MS.gene49161:CDS
GTTGAAGGTTGGAGCTCATT+TGG 0.304474 1.3:-68663751 MS.gene49161:CDS
AGTTGGGAGAAGCAGTTTAG+AGG 0.305193 1.3:-68663541 MS.gene49161:CDS
CTTATTAGAGGTGATAAAGT+TGG 0.314713 1.3:-68663558 MS.gene49161:CDS
AACCTCTTGTGTTTCTGGTT+TGG 0.324737 1.3:-68664072 MS.gene49161:CDS
GGATAGTGAGATTCTTGTTA+TGG 0.338333 1.3:-68664027 MS.gene49161:CDS
ATTCTTGTTATGGTTGGTGC+TGG 0.339331 1.3:-68664017 MS.gene49161:CDS
AAACACAAGAGGTTCTATAA+AGG 0.342979 1.3:+68664081 None:intergenic
TTCTTGTTATGGTTGGTGCT+GGG 0.346295 1.3:-68664016 MS.gene49161:CDS
TATGGTTGGTGCTGGGGCTC+TGG 0.359468 1.3:-68664009 MS.gene49161:CDS
GGTTGGAGCTCATTTGGATT+TGG 0.375125 1.3:-68663745 MS.gene49161:CDS
TTCTGCTGTTGTTGATATGC+TGG 0.375347 1.3:-68663493 MS.gene49161:CDS
AACTTGTTGGAGCTTATTAG+AGG 0.376708 1.3:-68663570 MS.gene49161:CDS
GGGAATTTGGATGAGGTTGT+TGG 0.406725 1.3:-68663834 MS.gene49161:CDS
TGATCAAGGAAGATGAAATT+GGG 0.408514 1.3:-68663596 MS.gene49161:CDS
AAAATGAGCTTTAATCAAAT+GGG 0.414889 1.3:+68663983 None:intergenic
TGTGAGCTGTGCTACTAATT+CGG 0.423913 1.3:-68663799 MS.gene49161:CDS
GTTGAAGCAGGGGAGCTTGT+GGG 0.429786 1.3:-68663636 MS.gene49161:CDS
TTATTAGAGGTGATAAAGTT+GGG 0.439432 1.3:-68663557 MS.gene49161:CDS
GGAGCTCATTTGGATTTGGT+TGG 0.440193 1.3:-68663741 MS.gene49161:CDS
AACACAAGAGGTTCTATAAA+GGG 0.442179 1.3:+68664082 None:intergenic
TTCATTTAAGCATTCAATGA+AGG 0.446956 1.3:-68663718 MS.gene49161:CDS
CAGAATTTACCAGGTGTTCA+AGG 0.463641 1.3:-68664170 MS.gene49161:CDS
AGCTAGGGTTTGACCAGTTG+TGG 0.467549 1.3:+68664127 None:intergenic
GGATGAGGTTGTTGGATTTG+GGG 0.471029 1.3:-68663826 MS.gene49161:CDS
GGGTTGTGGGAATTTGGATG+AGG 0.472644 1.3:-68663841 MS.gene49161:CDS
TGTTGACAATGAGGCTGCAT+TGG 0.473667 1.3:-68663658 MS.gene49161:CDS
CGGTTGAATCCATAGAAGCT+AGG 0.474193 1.3:+68664111 None:intergenic
TTGGAATAGGACAGTTGAAA+AGG 0.474625 1.3:-68663928 MS.gene49161:CDS
AGTTGAAAAGGCGAAAACTT+TGG 0.477139 1.3:-68663916 MS.gene49161:CDS
GGTTCTATAAAGGGTAAGTT+CGG 0.487217 1.3:+68664091 None:intergenic
GAGGCTGCATTGGTTGAAGC+AGG 0.488309 1.3:-68663648 MS.gene49161:CDS
ACACCTTCTTTCCCTTACAT+TGG 0.488935 1.3:-68664230 MS.gene49161:CDS
AGTGAGATTCTTGTTATGGT+TGG 0.493863 1.3:-68664023 MS.gene49161:CDS
TGATGATGAGGCTTTGAAAA+GGG 0.498048 1.3:-68663691 MS.gene49161:CDS
GGTTGAAGCAGGGGAGCTTG+TGG 0.506759 1.3:-68663637 MS.gene49161:CDS
ACACCAATGTAAGGGAAAGA+AGG 0.509618 1.3:+68664227 None:intergenic
TCTTGTTATGGTTGGTGCTG+GGG 0.518068 1.3:-68664015 MS.gene49161:CDS
GTGATGATGAGGCTTTGAAA+AGG 0.530047 1.3:-68663692 MS.gene49161:CDS
TTCGGAGACAGCGCTTGTGA+AGG 0.531883 1.3:-68663781 MS.gene49161:CDS
AACTCTTCACAGAATTTACC+AGG 0.535091 1.3:-68664179 MS.gene49161:CDS
AAGGGTGAGAGGTTGAAGGT+TGG 0.535869 1.3:-68663762 MS.gene49161:CDS
TCGGAGACAGCGCTTGTGAA+GGG 0.542077 1.3:-68663780 MS.gene49161:CDS
TTCTAAATATTTAGCTAGAG+AGG 0.543209 1.3:-68664048 MS.gene49161:CDS
GGTTGAATCCATAGAAGCTA+GGG 0.552130 1.3:+68664112 None:intergenic
TGTGAAGGGTGAGAGGTTGA+AGG 0.554444 1.3:-68663766 MS.gene49161:CDS
GGCTGCATTGGTTGAAGCAG+GGG 0.573870 1.3:-68663646 MS.gene49161:CDS
CAGCGCTTGTGAAGGGTGAG+AGG 0.592375 1.3:-68663773 MS.gene49161:CDS
AGGCTGCATTGGTTGAAGCA+GGG 0.600932 1.3:-68663647 MS.gene49161:CDS
ACATAGCTACCTTGAACACC+TGG 0.601923 1.3:+68664161 None:intergenic
TGAGACATACACAAGGACAT+AGG 0.603141 1.3:-68663454 None:intergenic
GATCAAGGAAGATGAAATTG+GGG 0.606167 1.3:-68663595 MS.gene49161:CDS
ATGATGAGGCTTTGAAAAGG+GGG 0.615537 1.3:-68663689 MS.gene49161:CDS
GATGATGAGGCTTTGAAAAG+GGG 0.633023 1.3:-68663690 MS.gene49161:CDS
AAATGAGCTTTAATCAAATG+GGG 0.636157 1.3:+68663984 None:intergenic
GAAGGTGTGGAAGACCAAGA+TGG 0.646483 1.3:+68664245 None:intergenic
AATGTAAGGGAAAGAAGGTG+TGG 0.648374 1.3:+68664232 None:intergenic
AATGAAGGAATGTGATGATG+AGG 0.651034 1.3:-68663703 MS.gene49161:CDS
AGCCAAACCAGAAACACAAG+AGG 0.653819 1.3:+68664070 None:intergenic
ATCAAGGAAGATGAAATTGG+GGG 0.695243 1.3:-68663594 MS.gene49161:CDS
TCAAGGAAGATGAAATTGGG+GGG 0.718689 1.3:-68663593 MS.gene49161:CDS
TTGTTTATGAGACATACACA+AGG 0.727977 1.3:-68663461 MS.gene49161:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAGTATTAATTTGGAAT+AGG - chr1.3:68663762-68663781 MS.gene49161:CDS 15.0%
!!! AATTAATACTTTTTTCAAAC+TGG + chr1.3:68663756-68663775 None:intergenic 15.0%
!!! ATTAATACTTTTTTCAAACT+GGG + chr1.3:68663755-68663774 None:intergenic 15.0%
!!! GTTTGAAAAAAGTATTAATT+TGG - chr1.3:68663756-68663775 MS.gene49161:CDS 15.0%
!!! AAAAATGAGCTTTAATCAAA+TGG + chr1.3:68663724-68663743 None:intergenic 20.0%
!!! AAAATGAGCTTTAATCAAAT+GGG + chr1.3:68663723-68663742 None:intergenic 20.0%
! TTATTAGAGGTGATAAAGTT+GGG - chr1.3:68664146-68664165 MS.gene49161:CDS 25.0%
! TTCATTTAAGCATTCAATGA+AGG - chr1.3:68663985-68664004 MS.gene49161:CDS 25.0%
! TTCTAAATATTTAGCTAGAG+AGG - chr1.3:68663655-68663674 MS.gene49161:CDS 25.0%
!! AAATGAGCTTTAATCAAATG+GGG + chr1.3:68663722-68663741 None:intergenic 25.0%
AAACACAAGAGGTTCTATAA+AGG + chr1.3:68663625-68663644 None:intergenic 30.0%
AACACAAGAGGTTCTATAAA+GGG + chr1.3:68663624-68663643 None:intergenic 30.0%
CTTATTAGAGGTGATAAAGT+TGG - chr1.3:68664145-68664164 MS.gene49161:CDS 30.0%
TGATCAAGGAAGATGAAATT+GGG - chr1.3:68664107-68664126 MS.gene49161:CDS 30.0%
TTGTTTATGAGACATACACA+AGG - chr1.3:68664242-68664261 MS.gene49161:CDS 30.0%
! AAATTCTGTGAAGAGTTTTC+AGG + chr1.3:68663521-68663540 None:intergenic 30.0%
! AATTCTGTGAAGAGTTTTCA+GGG + chr1.3:68663520-68663539 None:intergenic 30.0%
! GAAAGTTTTTGTTGACAATG+AGG - chr1.3:68664036-68664055 MS.gene49161:CDS 30.0%
!! TTAAAGCTCATTTTTCAGCT+AGG - chr1.3:68663729-68663748 MS.gene49161:CDS 30.0%
!!! ATTACTGTTTTCAAGTCTGT+TGG - chr1.3:68664187-68664206 MS.gene49161:CDS 30.0%
AACTCTTCACAGAATTTACC+AGG - chr1.3:68663524-68663543 MS.gene49161:CDS 35.0%
AGTTGAAAAGGCGAAAACTT+TGG - chr1.3:68663787-68663806 MS.gene49161:CDS 35.0%
ATCAAGGAAGATGAAATTGG+GGG - chr1.3:68664109-68664128 MS.gene49161:CDS 35.0%
GATCAAGGAAGATGAAATTG+GGG - chr1.3:68664108-68664127 MS.gene49161:CDS 35.0%
GGATAGTGAGATTCTTGTTA+TGG - chr1.3:68663676-68663695 MS.gene49161:CDS 35.0%
GGTTCTATAAAGGGTAAGTT+CGG + chr1.3:68663615-68663634 None:intergenic 35.0%
GTGATCAAGGAAGATGAAAT+TGG - chr1.3:68664106-68664125 MS.gene49161:CDS 35.0%
TATAGAACCTCTTGTGTTTC+TGG - chr1.3:68663626-68663645 MS.gene49161:CDS 35.0%
TTGGAATAGGACAGTTGAAA+AGG - chr1.3:68663775-68663794 MS.gene49161:CDS 35.0%
! AATGAAGGAATGTGATGATG+AGG - chr1.3:68664000-68664019 MS.gene49161:CDS 35.0%
! ACGAGTTTTACACCAATGTA+AGG + chr1.3:68663488-68663507 None:intergenic 35.0%
! AGCGATGAATTTTTGCTTTC+AGG - chr1.3:68663827-68663846 MS.gene49161:CDS 35.0%
! AGTGAGATTCTTGTTATGGT+TGG - chr1.3:68663680-68663699 MS.gene49161:CDS 35.0%
! CGAGTTTTACACCAATGTAA+GGG + chr1.3:68663487-68663506 None:intergenic 35.0%
! GCGATGAATTTTTGCTTTCA+GGG - chr1.3:68663828-68663847 MS.gene49161:CDS 35.0%
! GTTCTTTTCAATTCCACAAC+TGG - chr1.3:68663563-68663582 MS.gene49161:CDS 35.0%
!! AACTTGTTGGAGCTTATTAG+AGG - chr1.3:68664133-68664152 MS.gene49161:CDS 35.0%
!! TGAAGAGTTTTCAGGGAAAT+GGG + chr1.3:68663513-68663532 None:intergenic 35.0%
!! TGATGATGAGGCTTTGAAAA+GGG - chr1.3:68664012-68664031 MS.gene49161:CDS 35.0%
AATGTAAGGGAAAGAAGGTG+TGG + chr1.3:68663474-68663493 None:intergenic 40.0%
ACACCAATGTAAGGGAAAGA+AGG + chr1.3:68663479-68663498 None:intergenic 40.0%
ACACCTTCTTTCCCTTACAT+TGG - chr1.3:68663473-68663492 MS.gene49161:CDS 40.0%
CAGAATTTACCAGGTGTTCA+AGG - chr1.3:68663533-68663552 MS.gene49161:CDS 40.0%
GGTTGAATCCATAGAAGCTA+GGG + chr1.3:68663594-68663613 None:intergenic 40.0%
TCAAGGAAGATGAAATTGGG+GGG - chr1.3:68664110-68664129 MS.gene49161:CDS 40.0%
TGGATGAGGTTGTTGGATTT+GGG - chr1.3:68663876-68663895 MS.gene49161:CDS 40.0%
TTCTGCTGTTGTTGATATGC+TGG - chr1.3:68664210-68664229 MS.gene49161:CDS 40.0%
TTGGATGAGGTTGTTGGATT+TGG - chr1.3:68663875-68663894 MS.gene49161:CDS 40.0%
! ATTCTTGTTATGGTTGGTGC+TGG - chr1.3:68663686-68663705 MS.gene49161:CDS 40.0%
! TGTGAGCTGTGCTACTAATT+CGG - chr1.3:68663904-68663923 MS.gene49161:CDS 40.0%
! TTCTTGTTATGGTTGGTGCT+GGG - chr1.3:68663687-68663706 MS.gene49161:CDS 40.0%
!! AACCTCTTGTGTTTCTGGTT+TGG - chr1.3:68663631-68663650 MS.gene49161:CDS 40.0%
!! ATGATGAGGCTTTGAAAAGG+GGG - chr1.3:68664014-68664033 MS.gene49161:CDS 40.0%
!! CTTTCAGGGTTGAGTTTTGA+GGG - chr1.3:68663842-68663861 MS.gene49161:CDS 40.0%
!! GATGATGAGGCTTTGAAAAG+GGG - chr1.3:68664013-68664032 MS.gene49161:CDS 40.0%
!! GTGAAGAGTTTTCAGGGAAA+TGG + chr1.3:68663514-68663533 None:intergenic 40.0%
!! GTGATGATGAGGCTTTGAAA+AGG - chr1.3:68664011-68664030 MS.gene49161:CDS 40.0%
!! TTTTGAAAGGGGTGTGATCA+AGG - chr1.3:68664093-68664112 MS.gene49161:CDS 40.0%
!! TTTTGAGGGTTGTGGGAATT+TGG - chr1.3:68663856-68663875 MS.gene49161:CDS 40.0%
ACATAGCTACCTTGAACACC+TGG + chr1.3:68663545-68663564 None:intergenic 45.0%
AGCCAAACCAGAAACACAAG+AGG + chr1.3:68663636-68663655 None:intergenic 45.0%
AGTTGGGAGAAGCAGTTTAG+AGG - chr1.3:68664162-68664181 MS.gene49161:CDS 45.0%
CGGTTGAATCCATAGAAGCT+AGG + chr1.3:68663595-68663614 None:intergenic 45.0%
GGATGAGGTTGTTGGATTTG+GGG - chr1.3:68663877-68663896 MS.gene49161:CDS 45.0%
GGGAATTTGGATGAGGTTGT+TGG - chr1.3:68663869-68663888 MS.gene49161:CDS 45.0%
GGTTGGAGCTCATTTGGATT+TGG - chr1.3:68663958-68663977 MS.gene49161:CDS 45.0%
GTTGAAGGTTGGAGCTCATT+TGG - chr1.3:68663952-68663971 MS.gene49161:CDS 45.0%
TGAAATTGGGGGGAACTTGT+TGG - chr1.3:68664120-68664139 MS.gene49161:CDS 45.0%
TGGTCAAACCCTAGCTTCTA+TGG - chr1.3:68663583-68663602 MS.gene49161:CDS 45.0%
TGTTGACAATGAGGCTGCAT+TGG - chr1.3:68664045-68664064 MS.gene49161:CDS 45.0%
! AGCTTGTGGGTGCTTTTGAA+AGG - chr1.3:68664080-68664099 MS.gene49161:CDS 45.0%
! GCTTTCAGGGTTGAGTTTTG+AGG - chr1.3:68663841-68663860 MS.gene49161:CDS 45.0%
! GGAGCTCATTTGGATTTGGT+TGG - chr1.3:68663962-68663981 MS.gene49161:CDS 45.0%
!! CTTGTGGGTGCTTTTGAAAG+GGG - chr1.3:68664082-68664101 MS.gene49161:CDS 45.0%
!! GCTTGTGGGTGCTTTTGAAA+GGG - chr1.3:68664081-68664100 MS.gene49161:CDS 45.0%
!! TCTTGTTATGGTTGGTGCTG+GGG - chr1.3:68663688-68663707 MS.gene49161:CDS 45.0%
!!! GGTTGAGTTTTGAGGGTTGT+GGG - chr1.3:68663849-68663868 MS.gene49161:CDS 45.0%
AAGGGTGAGAGGTTGAAGGT+TGG - chr1.3:68663941-68663960 MS.gene49161:CDS 50.0%
AGCTAGGGTTTGACCAGTTG+TGG + chr1.3:68663579-68663598 None:intergenic 50.0%
GGGTTGTGGGAATTTGGATG+AGG - chr1.3:68663862-68663881 MS.gene49161:CDS 50.0%
TGTGAAGGGTGAGAGGTTGA+AGG - chr1.3:68663937-68663956 MS.gene49161:CDS 50.0%
! GAAGGTGTGGAAGACCAAGA+TGG + chr1.3:68663461-68663480 None:intergenic 50.0%
!! AGGCTGCATTGGTTGAAGCA+GGG - chr1.3:68664056-68664075 MS.gene49161:CDS 50.0%
!!! GGGTTGAGTTTTGAGGGTTG+TGG - chr1.3:68663848-68663867 MS.gene49161:CDS 50.0%
GTTGAAGCAGGGGAGCTTGT+GGG - chr1.3:68664067-68664086 MS.gene49161:CDS 55.0%
TCGGAGACAGCGCTTGTGAA+GGG - chr1.3:68663923-68663942 MS.gene49161:CDS 55.0%
TTCGGAGACAGCGCTTGTGA+AGG - chr1.3:68663922-68663941 MS.gene49161:CDS 55.0%
!! GAGGCTGCATTGGTTGAAGC+AGG - chr1.3:68664055-68664074 MS.gene49161:CDS 55.0%
!! GGCTGCATTGGTTGAAGCAG+GGG - chr1.3:68664057-68664076 MS.gene49161:CDS 55.0%
CAGCGCTTGTGAAGGGTGAG+AGG - chr1.3:68663930-68663949 MS.gene49161:CDS 60.0%
GGTTGAAGCAGGGGAGCTTG+TGG - chr1.3:68664066-68664085 MS.gene49161:CDS 60.0%
!! TATGGTTGGTGCTGGGGCTC+TGG - chr1.3:68663694-68663713 MS.gene49161:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 68663455 68664270 68663455 ID=MS.gene49161
chr1.3 mRNA 68663455 68664270 68663455 ID=MS.gene49161.t1;Parent=MS.gene49161
chr1.3 exon 68663455 68664270 68663455 ID=MS.gene49161.t1.exon1;Parent=MS.gene49161.t1
chr1.3 CDS 68663455 68664270 68663455 ID=cds.MS.gene49161.t1;Parent=MS.gene49161.t1
Gene Sequence

>MS.gene49161

ATGCCATCTTGGTCTTCCACACCTTCTTTCCCTTACATTGGTGTAAAACTCGTGACCCATTTCCCTGAAAACTCTTCACAGAATTTACCAGGTGTTCAAGGTAGCTATGTTCTTTTCAATTCCACAACTGGTCAAACCCTAGCTTCTATGGATTCAACCGAACTTACCCTTTATAGAACCTCTTGTGTTTCTGGTTTGGCTTCTAAATATTTAGCTAGAGAGGATAGTGAGATTCTTGTTATGGTTGGTGCTGGGGCTCTGGCACCCCATTTGATTAAAGCTCATTTTTCAGCTAGGCCCAGTTTGAAAAAAGTATTAATTTGGAATAGGACAGTTGAAAAGGCGAAAACTTTGGCGAAGAAATTGAGTGAAAGCGATGAATTTTTGCTTTCAGGGTTGAGTTTTGAGGGTTGTGGGAATTTGGATGAGGTTGTTGGATTTGGGGATATTGTGAGCTGTGCTACTAATTCGGAGACAGCGCTTGTGAAGGGTGAGAGGTTGAAGGTTGGAGCTCATTTGGATTTGGTTGGTTCATTTAAGCATTCAATGAAGGAATGTGATGATGAGGCTTTGAAAAGGGGGAAAGTTTTTGTTGACAATGAGGCTGCATTGGTTGAAGCAGGGGAGCTTGTGGGTGCTTTTGAAAGGGGTGTGATCAAGGAAGATGAAATTGGGGGGAACTTGTTGGAGCTTATTAGAGGTGATAAAGTTGGGAGAAGCAGTTTAGAGGAAATTACTGTTTTCAAGTCTGTTGGTTCTGCTGTTGTTGATATGCTGGCTGCACAGTTTGTTTATGAGACATACACAAGGACATAG

Protein sequence

>MS.gene49161.t1

MPSWSSTPSFPYIGVKLVTHFPENSSQNLPGVQGSYVLFNSTTGQTLASMDSTELTLYRTSCVSGLASKYLAREDSEILVMVGAGALAPHLIKAHFSARPSLKKVLIWNRTVEKAKTLAKKLSESDEFLLSGLSFEGCGNLDEVVGFGDIVSCATNSETALVKGERLKVGAHLDLVGSFKHSMKECDDEALKRGKVFVDNEAALVEAGELVGAFERGVIKEDEIGGNLLELIRGDKVGRSSLEEITVFKSVGSAVVDMLAAQFVYETYTRT