Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52438.t1 | XP_013463294.1 | 93.1 | 1635 | 76 | 5 | 1 | 1599 | 32 | 1665 | 0.00E+00 | 2838.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52438.t1 | A0A072VGM3 | 93.1 | 1635 | 76 | 5 | 1 | 1599 | 32 | 1665 | 0.0e+00 | 2838.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene52438 | 0.822878 | 1.87E-53 | -1.69E-46 |
| MS.gene049159 | MS.gene52438 | 0.82344 | 1.38E-53 | -1.69E-46 |
| MS.gene049435 | MS.gene52438 | 0.804938 | 1.70E-49 | -1.69E-46 |
| MS.gene049801 | MS.gene52438 | 0.818299 | 2.11E-52 | -1.69E-46 |
| MS.gene050654 | MS.gene52438 | 0.824141 | 9.48E-54 | -1.69E-46 |
| MS.gene050737 | MS.gene52438 | 0.816321 | 5.87E-52 | -1.69E-46 |
| MS.gene050941 | MS.gene52438 | 0.82287 | 1.88E-53 | -1.69E-46 |
| MS.gene051147 | MS.gene52438 | 0.809503 | 1.83E-50 | -1.69E-46 |
| MS.gene051209 | MS.gene52438 | 0.800692 | 1.28E-48 | -1.69E-46 |
| MS.gene051210 | MS.gene52438 | 0.800057 | 1.72E-48 | -1.69E-46 |
| MS.gene051800 | MS.gene52438 | 0.846623 | 1.93E-59 | -1.69E-46 |
| MS.gene052609 | MS.gene52438 | 0.829696 | 4.45E-55 | -1.69E-46 |
| MS.gene052674 | MS.gene52438 | 0.81003 | 1.41E-50 | -1.69E-46 |
| MS.gene052675 | MS.gene52438 | 0.810343 | 1.21E-50 | -1.69E-46 |
| MS.gene052677 | MS.gene52438 | 0.817843 | 2.67E-52 | -1.69E-46 |
| MS.gene052775 | MS.gene52438 | 0.808287 | 3.34E-50 | -1.69E-46 |
| MS.gene052776 | MS.gene52438 | 0.804413 | 2.19E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene52438 | 0.811609 | 6.43E-51 | -1.69E-46 |
| MS.gene053079 | MS.gene52438 | 0.821852 | 3.24E-53 | -1.69E-46 |
| MS.gene05350 | MS.gene52438 | 0.816636 | 4.99E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene52438 | 0.828546 | 8.46E-55 | -1.69E-46 |
| MS.gene054651 | MS.gene52438 | 0.801393 | 9.20E-49 | -1.69E-46 |
| MS.gene055864 | MS.gene52438 | 0.815784 | 7.74E-52 | -1.69E-46 |
| MS.gene055926 | MS.gene52438 | 0.833395 | 5.47E-56 | -1.69E-46 |
| MS.gene056507 | MS.gene52438 | 0.809876 | 1.52E-50 | -1.69E-46 |
| MS.gene057855 | MS.gene52438 | 0.805892 | 1.07E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene52438 | 0.833903 | 4.08E-56 | -1.69E-46 |
| MS.gene057915 | MS.gene52438 | 0.816279 | 6.00E-52 | -1.69E-46 |
| MS.gene058010 | MS.gene52438 | 0.816332 | 5.84E-52 | -1.69E-46 |
| MS.gene058081 | MS.gene52438 | 0.851753 | 7.23E-61 | -1.69E-46 |
| MS.gene058289 | MS.gene52438 | 0.805978 | 1.03E-49 | -1.69E-46 |
| MS.gene058292 | MS.gene52438 | 0.81225 | 4.66E-51 | -1.69E-46 |
| MS.gene058595 | MS.gene52438 | 0.80319 | 3.92E-49 | -1.69E-46 |
| MS.gene058647 | MS.gene52438 | 0.820242 | 7.61E-53 | -1.69E-46 |
| MS.gene058919 | MS.gene52438 | 0.842718 | 2.18E-58 | -1.69E-46 |
| MS.gene058979 | MS.gene52438 | 0.800557 | 1.36E-48 | -1.69E-46 |
| MS.gene059283 | MS.gene52438 | 0.827204 | 1.78E-54 | -1.69E-46 |
| MS.gene059439 | MS.gene52438 | 0.826592 | 2.49E-54 | -1.69E-46 |
| MS.gene059570 | MS.gene52438 | 0.812971 | 3.24E-51 | -1.69E-46 |
| MS.gene059725 | MS.gene52438 | 0.823399 | 1.41E-53 | -1.69E-46 |
| MS.gene05973 | MS.gene52438 | 0.814135 | 1.80E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene52438 | 0.806515 | 7.92E-50 | -1.69E-46 |
| MS.gene060296 | MS.gene52438 | 0.800071 | 1.71E-48 | -1.69E-46 |
| MS.gene060380 | MS.gene52438 | 0.810099 | 1.36E-50 | -1.69E-46 |
| MS.gene060435 | MS.gene52438 | 0.820665 | 6.08E-53 | -1.69E-46 |
| MS.gene06052 | MS.gene52438 | 0.840912 | 6.53E-58 | -1.69E-46 |
| MS.gene060637 | MS.gene52438 | 0.818951 | 1.50E-52 | -1.69E-46 |
| MS.gene060683 | MS.gene52438 | 0.818332 | 2.07E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene52438 | 0.819122 | 1.37E-52 | -1.69E-46 |
| MS.gene060777 | MS.gene52438 | 0.80008 | 1.70E-48 | -1.69E-46 |
| MS.gene060802 | MS.gene52438 | 0.807327 | 5.34E-50 | -1.69E-46 |
| MS.gene060933 | MS.gene52438 | 0.812158 | 4.88E-51 | -1.69E-46 |
| MS.gene06121 | MS.gene52438 | 0.81825 | 2.16E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52438.t1 | MTR_2g437350 | 93.089 | 1635 | 76 | 5 | 1 | 1599 | 32 | 1665 | 0.0 | 3057 |
| MS.gene52438.t1 | MTR_2g437350 | 92.885 | 1560 | 75 | 4 | 76 | 1599 | 1 | 1560 | 0.0 | 2905 |
| MS.gene52438.t1 | MTR_2g437370 | 82.143 | 196 | 21 | 2 | 559 | 740 | 1 | 196 | 2.91e-106 | 337 |
| MS.gene52438.t1 | MTR_7g108330 | 54.510 | 255 | 113 | 3 | 1338 | 1589 | 1409 | 1663 | 1.81e-74 | 275 |
| MS.gene52438.t1 | MTR_7g108330 | 34.074 | 405 | 240 | 10 | 1 | 396 | 71 | 457 | 2.64e-36 | 151 |
| MS.gene52438.t1 | MTR_2g437370 | 76.087 | 138 | 19 | 2 | 617 | 740 | 23 | 160 | 5.55e-61 | 206 |
| MS.gene52438.t1 | MTR_7g108330 | 34.074 | 405 | 240 | 10 | 1 | 396 | 62 | 448 | 2.30e-36 | 151 |
| MS.gene52438.t1 | MTR_7g108330 | 52.857 | 140 | 64 | 2 | 1338 | 1475 | 1400 | 1539 | 2.21e-34 | 145 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52438.t1 | AT4G15730 | 46.559 | 247 | 132 | 0 | 1352 | 1598 | 793 | 1039 | 3.42e-62 | 233 |
| MS.gene52438.t1 | AT4G15730 | 56.364 | 55 | 24 | 0 | 583 | 637 | 400 | 454 | 1.07e-16 | 87.0 |
| MS.gene52438.t1 | AT4G15730 | 46.559 | 247 | 132 | 0 | 1352 | 1598 | 812 | 1058 | 5.14e-62 | 232 |
| MS.gene52438.t1 | AT4G15730 | 56.364 | 55 | 24 | 0 | 583 | 637 | 419 | 473 | 9.81e-17 | 87.0 |
| MS.gene52438.t1 | AT3G62900 | 47.843 | 255 | 127 | 4 | 1340 | 1588 | 1146 | 1400 | 9.75e-59 | 224 |
| MS.gene52438.t1 | AT3G62900 | 54.369 | 103 | 45 | 1 | 551 | 653 | 541 | 641 | 2.97e-31 | 134 |
| MS.gene52438.t1 | AT3G62900 | 47.843 | 255 | 127 | 4 | 1340 | 1588 | 1146 | 1400 | 9.75e-59 | 224 |
| MS.gene52438.t1 | AT3G62900 | 54.369 | 103 | 45 | 1 | 551 | 653 | 541 | 641 | 2.97e-31 | 134 |
| MS.gene52438.t1 | AT3G62900 | 47.843 | 255 | 127 | 4 | 1340 | 1588 | 1146 | 1400 | 9.75e-59 | 224 |
| MS.gene52438.t1 | AT3G62900 | 54.369 | 103 | 45 | 1 | 551 | 653 | 541 | 641 | 2.97e-31 | 134 |
| MS.gene52438.t1 | AT1G02990 | 44.898 | 245 | 126 | 4 | 1348 | 1589 | 1038 | 1276 | 2.38e-51 | 199 |
| MS.gene52438.t1 | AT1G02990 | 34.694 | 245 | 131 | 7 | 1 | 242 | 52 | 270 | 1.06e-21 | 103 |
| MS.gene52438.t1 | AT1G02990 | 44.898 | 245 | 126 | 4 | 1348 | 1589 | 1038 | 1276 | 2.38e-51 | 199 |
| MS.gene52438.t1 | AT1G02990 | 34.694 | 245 | 131 | 7 | 1 | 242 | 52 | 270 | 1.06e-21 | 103 |
| MS.gene52438.t1 | AT1G02990 | 44.898 | 245 | 126 | 4 | 1348 | 1589 | 1038 | 1276 | 2.38e-51 | 199 |
| MS.gene52438.t1 | AT1G02990 | 34.694 | 245 | 131 | 7 | 1 | 242 | 52 | 270 | 1.06e-21 | 103 |
| MS.gene52438.t1 | AT1G02990 | 44.898 | 245 | 126 | 4 | 1348 | 1589 | 998 | 1236 | 2.49e-51 | 199 |
| MS.gene52438.t1 | AT1G02990 | 44.681 | 188 | 96 | 3 | 1343 | 1528 | 1033 | 1214 | 1.61e-35 | 148 |
| MS.gene52438.t1 | AT1G02990 | 34.694 | 245 | 131 | 7 | 1 | 242 | 52 | 270 | 1.37e-21 | 103 |
Find 0 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 15186 | gene | 3414 | 11192 | 3414 | ID=MS.gene52438 |
| 15186 | mRNA | 3414 | 11192 | 3414 | ID=MS.gene52438.t1;Parent=MS.gene52438 |
| 15186 | exon | 3414 | 3486 | 3414 | ID=MS.gene52438.t1.exon1;Parent=MS.gene52438.t1 |
| 15186 | CDS | 3414 | 3486 | 3414 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 3589 | 3701 | 3589 | ID=MS.gene52438.t1.exon2;Parent=MS.gene52438.t1 |
| 15186 | CDS | 3589 | 3701 | 3589 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 4152 | 4577 | 4152 | ID=MS.gene52438.t1.exon3;Parent=MS.gene52438.t1 |
| 15186 | CDS | 4152 | 4577 | 4152 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 4657 | 5698 | 4657 | ID=MS.gene52438.t1.exon4;Parent=MS.gene52438.t1 |
| 15186 | CDS | 4657 | 5698 | 4657 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 5734 | 5926 | 5734 | ID=MS.gene52438.t1.exon5;Parent=MS.gene52438.t1 |
| 15186 | CDS | 5734 | 5926 | 5734 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 6311 | 6494 | 6311 | ID=MS.gene52438.t1.exon6;Parent=MS.gene52438.t1 |
| 15186 | CDS | 6311 | 6494 | 6311 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 6535 | 6721 | 6535 | ID=MS.gene52438.t1.exon7;Parent=MS.gene52438.t1 |
| 15186 | CDS | 6535 | 6721 | 6535 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 6830 | 8715 | 6830 | ID=MS.gene52438.t1.exon8;Parent=MS.gene52438.t1 |
| 15186 | CDS | 6830 | 8715 | 6830 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 8808 | 8965 | 8808 | ID=MS.gene52438.t1.exon9;Parent=MS.gene52438.t1 |
| 15186 | CDS | 8808 | 8965 | 8808 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 9894 | 10054 | 9894 | ID=MS.gene52438.t1.exon10;Parent=MS.gene52438.t1 |
| 15186 | CDS | 9894 | 10054 | 9894 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 10179 | 10336 | 10179 | ID=MS.gene52438.t1.exon11;Parent=MS.gene52438.t1 |
| 15186 | CDS | 10179 | 10336 | 10179 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| 15186 | exon | 10974 | 11192 | 10974 | ID=MS.gene52438.t1.exon12;Parent=MS.gene52438.t1 |
| 15186 | CDS | 10974 | 11192 | 10974 | ID=cds.MS.gene52438.t1;Parent=MS.gene52438.t1 |
| Gene Sequence |
| Protein sequence |
