Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54473.t1 | RHN44081.1 | 89.2 | 93 | 10 | 0 | 1 | 93 | 5 | 97 | 4.30E-34 | 153.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54473.t1 | A0A072TWH2 | 89.2 | 93 | 10 | 0 | 1 | 93 | 18 | 110 | 3.1e-34 | 153.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049293 | MS.gene54473 | -0.81069 | 1.02E-50 | -1.69E-46 |
| MS.gene050277 | MS.gene54473 | 0.829849 | 4.09E-55 | -1.69E-46 |
| MS.gene050303 | MS.gene54473 | 0.845189 | 4.74E-59 | -1.69E-46 |
| MS.gene050623 | MS.gene54473 | -0.80007 | 1.71E-48 | -1.69E-46 |
| MS.gene051243 | MS.gene54473 | 0.804494 | 2.10E-49 | -1.69E-46 |
| MS.gene051245 | MS.gene54473 | 0.802883 | 4.54E-49 | -1.69E-46 |
| MS.gene051349 | MS.gene54473 | 0.801999 | 6.91E-49 | -1.69E-46 |
| MS.gene052286 | MS.gene54473 | 0.816442 | 5.52E-52 | -1.69E-46 |
| MS.gene052302 | MS.gene54473 | 0.812362 | 4.40E-51 | -1.69E-46 |
| MS.gene052309 | MS.gene54473 | -0.800625 | 1.32E-48 | -1.69E-46 |
| MS.gene052376 | MS.gene54473 | 0.818472 | 1.93E-52 | -1.69E-46 |
| MS.gene054657 | MS.gene54473 | 0.841358 | 4.99E-58 | -1.69E-46 |
| MS.gene055808 | MS.gene54473 | 0.809524 | 1.81E-50 | -1.69E-46 |
| MS.gene056631 | MS.gene54473 | 0.828555 | 8.42E-55 | -1.69E-46 |
| MS.gene056760 | MS.gene54473 | 0.818752 | 1.66E-52 | -1.69E-46 |
| MS.gene057060 | MS.gene54473 | 0.814073 | 1.85E-51 | -1.69E-46 |
| MS.gene057079 | MS.gene54473 | 0.826355 | 2.84E-54 | -1.69E-46 |
| MS.gene057555 | MS.gene54473 | 0.83039 | 3.02E-55 | -1.69E-46 |
| MS.gene05795 | MS.gene54473 | 0.819202 | 1.31E-52 | -1.69E-46 |
| MS.gene058238 | MS.gene54473 | 0.804652 | 1.95E-49 | -1.69E-46 |
| MS.gene059317 | MS.gene54473 | 0.848382 | 6.35E-60 | -1.69E-46 |
| MS.gene05954 | MS.gene54473 | 0.803255 | 3.80E-49 | -1.69E-46 |
| MS.gene059569 | MS.gene54473 | 0.815731 | 7.96E-52 | -1.69E-46 |
| MS.gene06001 | MS.gene54473 | 0.80029 | 1.54E-48 | -1.69E-46 |
| MS.gene060297 | MS.gene54473 | 0.806602 | 7.60E-50 | -1.69E-46 |
| MS.gene060429 | MS.gene54473 | 0.810027 | 1.41E-50 | -1.69E-46 |
| MS.gene060586 | MS.gene54473 | 0.801308 | 9.58E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54473.t1 | MTR_7g007290 | 89.247 | 93 | 10 | 0 | 1 | 93 | 18 | 110 | 1.78e-55 | 167 |
| MS.gene54473.t1 | MTR_7g007300 | 80.000 | 70 | 14 | 0 | 1 | 70 | 1 | 70 | 2.78e-31 | 114 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54473.t1 | AT1G47820 | 48.936 | 94 | 47 | 1 | 1 | 93 | 1 | 94 | 3.80e-20 | 77.8 |
| MS.gene54473.t1 | AT1G47820 | 48.936 | 94 | 47 | 1 | 1 | 93 | 1 | 94 | 3.80e-20 | 77.8 |
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACAGAGTCATATTCTGTTC+AGG | 0.414511 | 7.4:+92259195 | MS.gene54473:CDS |
| GTGGCATTCACCTGAGGTCT+AGG | 0.432602 | 7.4:-92259160 | None:intergenic |
| ACAAAGCATGGCCGTATTGA+AGG | 0.443308 | 7.4:+92259264 | MS.gene54473:CDS |
| TGTTATGAAATCCTTCAATA+CGG | 0.467267 | 7.4:-92259275 | None:intergenic |
| TATTCTGTTCAGGCAATCAA+TGG | 0.473465 | 7.4:+92259205 | MS.gene54473:CDS |
| TGACGATGATGTTGCTAAGA+AGG | 0.498240 | 7.4:+92259354 | MS.gene54473:CDS |
| TGCAGTGCTTCCTAGACCTC+AGG | 0.506425 | 7.4:+92259150 | MS.gene54473:CDS |
| GAAGCTTCTAACGACTCGTC+TGG | 0.528210 | 7.4:-92259322 | None:intergenic |
| TGCTAAGAAGGCTCTAGTAA+AGG | 0.543863 | 7.4:+92259366 | MS.gene54473:CDS |
| CGAGTCGTTAGAAGCTTCCT+CGG | 0.555746 | 7.4:+92259327 | MS.gene54473:CDS |
| GACTCGTCTGGAACATCTTG+AGG | 0.569302 | 7.4:-92259310 | None:intergenic |
| TAACATGAAAGAACAAAGCA+TGG | 0.616394 | 7.4:+92259252 | MS.gene54473:CDS |
| AACATCATCGTCATCTTCCG+AGG | 0.626307 | 7.4:-92259344 | None:intergenic |
| CGAAATGTGGCATTCACCTG+AGG | 0.643629 | 7.4:-92259166 | None:intergenic |
| CTCTGTGCTGAGACGAAATG+TGG | 0.644541 | 7.4:-92259179 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGTTATGAAATCCTTCAATA+CGG | - | chr7.4:92259278-92259297 | None:intergenic | 25.0% |
| TAACATGAAAGAACAAAGCA+TGG | + | chr7.4:92259252-92259271 | MS.gene54473:CDS | 30.0% | |
| TATTCTGTTCAGGCAATCAA+TGG | + | chr7.4:92259205-92259224 | MS.gene54473:CDS | 35.0% | |
| CACAGAGTCATATTCTGTTC+AGG | + | chr7.4:92259195-92259214 | MS.gene54473:CDS | 40.0% | |
| TGACGATGATGTTGCTAAGA+AGG | + | chr7.4:92259354-92259373 | MS.gene54473:CDS | 40.0% | |
| ! | TGCTAAGAAGGCTCTAGTAA+AGG | + | chr7.4:92259366-92259385 | MS.gene54473:CDS | 40.0% |
| AACATCATCGTCATCTTCCG+AGG | - | chr7.4:92259347-92259366 | None:intergenic | 45.0% | |
| ACAAAGCATGGCCGTATTGA+AGG | + | chr7.4:92259264-92259283 | MS.gene54473:CDS | 45.0% | |
| CGAAATGTGGCATTCACCTG+AGG | - | chr7.4:92259169-92259188 | None:intergenic | 50.0% | |
| CGAGTCGTTAGAAGCTTCCT+CGG | + | chr7.4:92259327-92259346 | MS.gene54473:CDS | 50.0% | |
| CTCTGTGCTGAGACGAAATG+TGG | - | chr7.4:92259182-92259201 | None:intergenic | 50.0% | |
| GAAGCTTCTAACGACTCGTC+TGG | - | chr7.4:92259325-92259344 | None:intergenic | 50.0% | |
| ! | GACTCGTCTGGAACATCTTG+AGG | - | chr7.4:92259313-92259332 | None:intergenic | 50.0% |
| GTGGCATTCACCTGAGGTCT+AGG | - | chr7.4:92259163-92259182 | None:intergenic | 55.0% | |
| TGCAGTGCTTCCTAGACCTC+AGG | + | chr7.4:92259150-92259169 | MS.gene54473:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 92259130 | 92259411 | 92259130 | ID=MS.gene54473 |
| chr7.4 | mRNA | 92259130 | 92259411 | 92259130 | ID=MS.gene54473.t1;Parent=MS.gene54473 |
| chr7.4 | exon | 92259130 | 92259411 | 92259130 | ID=MS.gene54473.t1.exon1;Parent=MS.gene54473.t1 |
| chr7.4 | CDS | 92259130 | 92259411 | 92259130 | ID=cds.MS.gene54473.t1;Parent=MS.gene54473.t1 |
| Gene Sequence |
| Protein sequence |
