Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56739.t1 | XP_003618426.1 | 89.8 | 59 | 6 | 0 | 7 | 65 | 237 | 295 | 1.30E-22 | 115.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56739.t1 | G7KK05 | 89.8 | 59 | 6 | 0 | 7 | 65 | 237 | 295 | 9.3e-23 | 115.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene56739 | 0.834264 | 3.31E-56 | -1.69E-46 |
| MS.gene050301 | MS.gene56739 | 0.84492 | 5.61E-59 | -1.69E-46 |
| MS.gene050529 | MS.gene56739 | 0.800883 | 1.17E-48 | -1.69E-46 |
| MS.gene05101 | MS.gene56739 | 0.840795 | 7.01E-58 | -1.69E-46 |
| MS.gene051471 | MS.gene56739 | 0.823153 | 1.61E-53 | -1.69E-46 |
| MS.gene052078 | MS.gene56739 | 0.835255 | 1.87E-56 | -1.69E-46 |
| MS.gene052178 | MS.gene56739 | 0.833238 | 5.98E-56 | -1.69E-46 |
| MS.gene052188 | MS.gene56739 | 0.82668 | 2.38E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene56739 | 0.816089 | 6.62E-52 | -1.69E-46 |
| MS.gene052310 | MS.gene56739 | 0.823133 | 1.63E-53 | -1.69E-46 |
| MS.gene052340 | MS.gene56739 | 0.826212 | 3.07E-54 | -1.69E-46 |
| MS.gene053557 | MS.gene56739 | 0.827606 | 1.43E-54 | -1.69E-46 |
| MS.gene053836 | MS.gene56739 | 0.831833 | 1.33E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene56739 | 0.824337 | 8.52E-54 | -1.69E-46 |
| MS.gene055390 | MS.gene56739 | 0.801023 | 1.09E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene56739 | 0.842224 | 2.95E-58 | -1.69E-46 |
| MS.gene055428 | MS.gene56739 | 0.821212 | 4.55E-53 | -1.69E-46 |
| MS.gene056612 | MS.gene56739 | 0.804295 | 2.31E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene56739 | -0.804293 | 2.32E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene56739 | 0.807178 | 5.74E-50 | -1.69E-46 |
| MS.gene057121 | MS.gene56739 | 0.808908 | 2.46E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene56739 | 0.838405 | 2.93E-57 | -1.69E-46 |
| MS.gene05811 | MS.gene56739 | 0.836667 | 8.18E-57 | -1.69E-46 |
| MS.gene058142 | MS.gene56739 | 0.810923 | 9.05E-51 | -1.69E-46 |
| MS.gene058276 | MS.gene56739 | 0.812425 | 4.27E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene56739 | 0.80423 | 2.39E-49 | -1.69E-46 |
| MS.gene058845 | MS.gene56739 | 0.822811 | 1.94E-53 | -1.69E-46 |
| MS.gene05886 | MS.gene56739 | 0.809106 | 2.23E-50 | -1.69E-46 |
| MS.gene058928 | MS.gene56739 | 0.8118 | 5.84E-51 | -1.69E-46 |
| MS.gene05907 | MS.gene56739 | 0.82378 | 1.15E-53 | -1.69E-46 |
| MS.gene059285 | MS.gene56739 | 0.807102 | 5.95E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene56739 | 0.848417 | 6.21E-60 | -1.69E-46 |
| MS.gene059597 | MS.gene56739 | 0.815661 | 8.24E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene56739 | 0.802332 | 5.90E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene56739 | 0.84991 | 2.39E-60 | -1.69E-46 |
| MS.gene059894 | MS.gene56739 | 0.855522 | 5.96E-62 | -1.69E-46 |
| MS.gene060407 | MS.gene56739 | 0.805965 | 1.03E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene56739 | 0.804132 | 2.50E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene56739 | 0.84177 | 3.88E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56739.t1 | MTR_6g009400 | 85.484 | 62 | 9 | 0 | 4 | 65 | 234 | 295 | 4.94e-33 | 116 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56739.t1 | AT3G28460 | 69.355 | 62 | 19 | 0 | 4 | 65 | 184 | 245 | 5.17e-24 | 92.0 |
| MS.gene56739.t1 | AT3G28460 | 69.355 | 62 | 19 | 0 | 4 | 65 | 229 | 290 | 8.81e-24 | 92.0 |
| MS.gene56739.t1 | AT3G28460 | 69.565 | 46 | 14 | 0 | 4 | 49 | 229 | 274 | 2.93e-15 | 69.3 |
Find 26 sgRNAs with CRISPR-Local
Find 52 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGCAGAATCACCCTTTGT+TGG | 0.387350 | 6.2:-4912317 | MS.gene56739:CDS |
| GCATAGTCAACTTCGGCATA+TGG | 0.424658 | 6.2:+4912356 | None:intergenic |
| GAAAGTGTCTTCTCCAACAA+AGG | 0.426593 | 6.2:+4912304 | None:intergenic |
| ATCTTGCGGACCCCTTGTTA+AGG | 0.459960 | 6.2:-4912155 | MS.gene56739:intron |
| AGTATGCTAAGAAAATTGTT+TGG | 0.481021 | 6.2:-4912688 | MS.gene56739:intron |
| TCCTTCGAAAACCGACATGC+TGG | 0.483247 | 6.2:-4912179 | MS.gene56739:CDS |
| GCTGCAATTGCGGCAGTGCG+TGG | 0.504631 | 6.2:-4912016 | MS.gene56739:CDS |
| TGCGGCAGTGCGTGGCAAAG+TGG | 0.544775 | 6.2:-4912008 | MS.gene56739:CDS |
| AAAGTGTCTTCTCCAACAAA+GGG | 0.549863 | 6.2:+4912305 | None:intergenic |
| TACTTGAATTACCTTAACAA+GGG | 0.575169 | 6.2:+4912144 | None:intergenic |
| AGTCAACTTCGGCATATGGA+GGG | 0.580092 | 6.2:+4912360 | None:intergenic |
| TTACTTGAATTACCTTAACA+AGG | 0.592062 | 6.2:+4912143 | None:intergenic |
| ACCGACATGCTGGAATCTTG+CGG | 0.595355 | 6.2:-4912169 | MS.gene56739:CDS |
| TAGTCAACTTCGGCATATGG+AGG | 0.596815 | 6.2:+4912359 | None:intergenic |
| GTCAACTTCGGCATATGGAG+GGG | 0.598620 | 6.2:+4912361 | None:intergenic |
| TTGACTATGCGGTGTTGATG+AGG | 0.601118 | 6.2:-4912343 | MS.gene56739:CDS |
| ACTTGAATTACCTTAACAAG+GGG | 0.605260 | 6.2:+4912145 | None:intergenic |
| AGTGGTGGTTGCTGCAATTG+CGG | 0.606677 | 6.2:-4912026 | MS.gene56739:CDS |
| TCCGCAAGATTCCAGCATGT+CGG | 0.609206 | 6.2:+4912168 | None:intergenic |
| GGCAGTGCGTGGCAAAGTGG+AGG | 0.613542 | 6.2:-4912005 | MS.gene56739:CDS |
| TCTGTAGTCAATAGCAGTGG+TGG | 0.616065 | 6.2:-4912041 | MS.gene56739:CDS |
| CAACACCGCATAGTCAACTT+CGG | 0.627785 | 6.2:+4912349 | None:intergenic |
| TGTTCACATGCAGGAAACAG+TGG | 0.638456 | 6.2:-4912407 | MS.gene56739:intron |
| GTCAGAGCAGATCTGCGTTG+CGG | 0.648573 | 6.2:-4911983 | MS.gene56739:CDS |
| ATATCTGTAGTCAATAGCAG+TGG | 0.690189 | 6.2:-4912044 | MS.gene56739:CDS |
| ATATGCCGAAGTTGACTATG+CGG | 0.710556 | 6.2:-4912354 | MS.gene56739:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCTTACTTTCTTATTAT+TGG | - | chr6.2:4912397-4912416 | MS.gene56739:CDS | 15.0% |
| !!! | TGTTTTGACAAAAAAAATAA+AGG | - | chr6.2:4912209-4912228 | MS.gene56739:intron | 15.0% |
| !! | AAGAAAACATATATGAGAAA+TGG | + | chr6.2:4912172-4912191 | None:intergenic | 20.0% |
| ! | TACTTGAATTACCTTAACAA+GGG | + | chr6.2:4912524-4912543 | None:intergenic | 25.0% |
| ! | TCTTTCTATTATTCAAGCTT+AGG | + | chr6.2:4912134-4912153 | None:intergenic | 25.0% |
| ! | TTACTTGAATTACCTTAACA+AGG | + | chr6.2:4912525-4912544 | None:intergenic | 25.0% |
| !!! | TATTGGTCTAAATAGTCTTT+TGG | - | chr6.2:4912414-4912433 | MS.gene56739:intron | 25.0% |
| AATTTGAATTGTTCACATGC+AGG | - | chr6.2:4912249-4912268 | MS.gene56739:intron | 30.0% | |
| ACTTGAATTACCTTAACAAG+GGG | + | chr6.2:4912523-4912542 | None:intergenic | 30.0% | |
| CATATATGAGAAATGGAAAG+TGG | + | chr6.2:4912165-4912184 | None:intergenic | 30.0% | |
| GCTAAGAAAATTGTTTGGTA+TGG | - | chr6.2:4911982-4912001 | MS.gene56739:CDS | 30.0% | |
| ! | GCAAAACATGCAAAATTTTG+TGG | - | chr6.2:4912570-4912589 | MS.gene56739:intron | 30.0% |
| !! | GAACTCTCATGTTTTTATCT+AGG | - | chr6.2:4912453-4912472 | MS.gene56739:intron | 30.0% |
| AAATTGTTTGGTATGGCTCA+TGG | - | chr6.2:4911989-4912008 | MS.gene56739:CDS | 35.0% | |
| ATATCTGTAGTCAATAGCAG+TGG | - | chr6.2:4912621-4912640 | MS.gene56739:intron | 35.0% | |
| CAGATATTACCTGACATATG+TGG | + | chr6.2:4912608-4912627 | None:intergenic | 35.0% | |
| CTCATGGTTTACTGATAAGT+GGG | - | chr6.2:4912005-4912024 | MS.gene56739:CDS | 35.0% | |
| ! | AAAGTGTCTTCTCCAACAAA+GGG | + | chr6.2:4912363-4912382 | None:intergenic | 35.0% |
| ATATGCCGAAGTTGACTATG+CGG | - | chr6.2:4912311-4912330 | MS.gene56739:CDS | 40.0% | |
| ATTGCAGAATCACCCTTTGT+TGG | - | chr6.2:4912348-4912367 | MS.gene56739:CDS | 40.0% | |
| GCTCATGGTTTACTGATAAG+TGG | - | chr6.2:4912004-4912023 | MS.gene56739:CDS | 40.0% | |
| TGGTTTACTGATAAGTGGGA+AGG | - | chr6.2:4912009-4912028 | MS.gene56739:CDS | 40.0% | |
| !! | GAAAGTGTCTTCTCCAACAA+AGG | + | chr6.2:4912364-4912383 | None:intergenic | 40.0% |
| AGTCAACTTCGGCATATGGA+GGG | + | chr6.2:4912308-4912327 | None:intergenic | 45.0% | |
| CAACACCGCATAGTCAACTT+CGG | + | chr6.2:4912319-4912338 | None:intergenic | 45.0% | |
| CTTGTTGCACCACATATGTC+AGG | - | chr6.2:4912596-4912615 | MS.gene56739:intron | 45.0% | |
| GCATAGTCAACTTCGGCATA+TGG | + | chr6.2:4912312-4912331 | None:intergenic | 45.0% | |
| TAGTCAACTTCGGCATATGG+AGG | + | chr6.2:4912309-4912328 | None:intergenic | 45.0% | |
| TCTGTAGTCAATAGCAGTGG+TGG | - | chr6.2:4912624-4912643 | MS.gene56739:intron | 45.0% | |
| TGTTCACATGCAGGAAACAG+TGG | - | chr6.2:4912258-4912277 | MS.gene56739:intron | 45.0% | |
| !! | TTGACTATGCGGTGTTGATG+AGG | - | chr6.2:4912322-4912341 | MS.gene56739:CDS | 45.0% |
| ACCGACATGCTGGAATCTTG+CGG | - | chr6.2:4912496-4912515 | MS.gene56739:intron | 50.0% | |
| AGTGGTGGTTGCTGCAATTG+CGG | - | chr6.2:4912639-4912658 | MS.gene56739:intron | 50.0% | |
| ATCTTGCGGACCCCTTGTTA+AGG | - | chr6.2:4912510-4912529 | MS.gene56739:intron | 50.0% | |
| GTCAACTTCGGCATATGGAG+GGG | + | chr6.2:4912307-4912326 | None:intergenic | 50.0% | |
| TCCGCAAGATTCCAGCATGT+CGG | + | chr6.2:4912500-4912519 | None:intergenic | 50.0% | |
| TCCTTCGAAAACCGACATGC+TGG | - | chr6.2:4912486-4912505 | MS.gene56739:intron | 50.0% | |
| !!! | TCCAGCATGTCGGTTTTCGA+AGG | + | chr6.2:4912490-4912509 | None:intergenic | 50.0% |
| AGTGGGAAGGTTGCTTGAGC+CGG | - | chr6.2:4912022-4912041 | MS.gene56739:CDS | 55.0% | |
| CTTCGCTGCGGCACGATTTA+GGG | - | chr6.2:4912057-4912076 | MS.gene56739:intron | 55.0% | |
| GTCAGAGCAGATCTGCGTTG+CGG | - | chr6.2:4912682-4912701 | MS.gene56739:intron | 55.0% | |
| TCTTCGCTGCGGCACGATTT+AGG | - | chr6.2:4912056-4912075 | MS.gene56739:intron | 55.0% | |
| TTCGCTGCGGCACGATTTAG+GGG | - | chr6.2:4912058-4912077 | MS.gene56739:intron | 55.0% | |
| AAGGTTGCTTGAGCCGGCGA+AGG | - | chr6.2:4912028-4912047 | MS.gene56739:CDS | 60.0% | |
| AGCGAAGAAACCCCCTTCGC+CGG | + | chr6.2:4912044-4912063 | None:intergenic | 60.0% | |
| AGGTTGCTTGAGCCGGCGAA+GGG | - | chr6.2:4912029-4912048 | MS.gene56739:CDS | 60.0% | |
| CGAAGGGGGTTTCTTCGCTG+CGG | - | chr6.2:4912045-4912064 | MS.gene56739:CDS | 60.0% | |
| GCTGCAATTGCGGCAGTGCG+TGG | - | chr6.2:4912649-4912668 | MS.gene56739:intron | 65.0% | |
| GGCAGTGCGTGGCAAAGTGG+AGG | - | chr6.2:4912660-4912679 | MS.gene56739:intron | 65.0% | |
| GGTTGCTTGAGCCGGCGAAG+GGG | - | chr6.2:4912030-4912049 | MS.gene56739:CDS | 65.0% | |
| GTTGCTTGAGCCGGCGAAGG+GGG | - | chr6.2:4912031-4912050 | MS.gene56739:CDS | 65.0% | |
| TGCGGCAGTGCGTGGCAAAG+TGG | - | chr6.2:4912657-4912676 | MS.gene56739:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 4911980 | 4912707 | 4911980 | ID=MS.gene56739 |
| chr6.2 | mRNA | 4911980 | 4912707 | 4911980 | ID=MS.gene56739.t1;Parent=MS.gene56739 |
| chr6.2 | exon | 4912689 | 4912707 | 4912689 | ID=MS.gene56739.t1.exon1;Parent=MS.gene56739.t1 |
| chr6.2 | CDS | 4912689 | 4912707 | 4912689 | ID=cds.MS.gene56739.t1;Parent=MS.gene56739.t1 |
| chr6.2 | exon | 4912301 | 4912416 | 4912301 | ID=MS.gene56739.t1.exon2;Parent=MS.gene56739.t1 |
| chr6.2 | CDS | 4912301 | 4912416 | 4912301 | ID=cds.MS.gene56739.t1;Parent=MS.gene56739.t1 |
| chr6.2 | exon | 4912156 | 4912212 | 4912156 | ID=MS.gene56739.t1.exon3;Parent=MS.gene56739.t1 |
| chr6.2 | CDS | 4912156 | 4912212 | 4912156 | ID=cds.MS.gene56739.t1;Parent=MS.gene56739.t1 |
| chr6.2 | exon | 4911980 | 4912069 | 4911980 | ID=MS.gene56739.t1.exon4;Parent=MS.gene56739.t1 |
| chr6.2 | CDS | 4911980 | 4912069 | 4911980 | ID=cds.MS.gene56739.t1;Parent=MS.gene56739.t1 |
| Gene Sequence |
| Protein sequence |
