AlfalfaGEDB | MS.gene60129

M. sativa cultivar XinJiangDaYe / MS.gene60129

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF01535.23 PF12854.10 PF13041.9 PF17177.7

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60129.t1	XP_024627797.1	95.7	162	7	0	4	165	15	176	1.80E-82	317

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60129.t1	Q9FKR3	55.4	175	74	3	5	175	15	189	1.1e-43	179.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60129.t1	G7LG30	95.7	162	7	0	4	165	15	176	1.3e-82	317.0

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052582	MS.gene60129	0.800281	1.55E-48	-1.69E-46
MS.gene052584	MS.gene60129	0.80774	4.36E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene000715	MS.gene60129	PPI
MS.gene003502	MS.gene60129	PPI
MS.gene006820	MS.gene60129	PPI
MS.gene012908	MS.gene60129	PPI
MS.gene020386	MS.gene60129	PPI
MS.gene029278	MS.gene60129	PPI
MS.gene037288	MS.gene60129	PPI
MS.gene038085	MS.gene60129	PPI
MS.gene040675	MS.gene60129	PPI
MS.gene048087	MS.gene60129	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60129.t1	MTR_8g039270	96.696	575	19	0	4	578	15	589	0.0	1171
MS.gene60129.t1	MTR_5g077930	30.906	508	342	4	20	518	50	557	3.35e-82	274
MS.gene60129.t1	MTR_5g077930	32.759	290	193	1	254	541	221	510	1.88e-48	181
MS.gene60129.t1	MTR_5g077930	22.685	216	166	1	327	541	155	370	5.99e-13	72.4
MS.gene60129.t1	MTR_4g074390	30.354	537	364	4	2	533	18	549	3.25e-81	266
MS.gene60129.t1	MTR_4g074390	33.937	221	140	2	125	341	349	567	1.27e-32	132
MS.gene60129.t1	MTR_4g108060	29.795	537	367	4	2	533	18	549	2.52e-80	264
MS.gene60129.t1	MTR_4g108060	34.842	221	138	2	125	341	349	567	5.49e-34	136
MS.gene60129.t1	MTR_1g033960	29.354	511	351	4	38	541	41	548	6.81e-77	258
MS.gene60129.t1	MTR_1g033960	30.534	393	262	4	191	573	121	512	1.04e-58	209

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60129.t1	AT5G38730	56.627	581	247	4	5	580	15	595	0.0	695
MS.gene60129.t1	AT1G09680	28.346	508	348	5	22	519	71	572	4.44e-74	248
MS.gene60129.t1	AT1G09680	29.012	324	226	2	254	573	235	558	3.09e-45	169
MS.gene60129.t1	AT1G09680	21.327	211	164	1	365	573	208	418	3.76e-13	72.8
MS.gene60129.t1	AT5G39710	29.116	498	346	4	49	541	64	559	4.39e-71	243
MS.gene60129.t1	AT5G39710	28.132	455	269	8	129	545	250	684	8.32e-54	195
MS.gene60129.t1	AT5G39710	24.634	410	250	3	116	466	335	744	8.91e-38	149
MS.gene60129.t1	AT5G39710	25.497	302	206	3	115	399	448	747	3.16e-22	101
MS.gene60129.t1	AT1G05670	29.455	550	335	10	22	528	48	587	1.83e-68	236
MS.gene60129.t1	AT1G05670	30.968	465	310	3	115	570	279	741	3.18e-67	232

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Find 112 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATGAGAATTAACTTC+CGG	0.514800	8.4:-59050961	None:intergenic
AAAACTAAGATGTTAGAAGC+TGG	0.510783	8.4:+59051771	MS.gene60129:CDS
AAACATGAGAATTAACTTCC+GGG	0.442431	8.4:-59050960	None:intergenic
AAATGCTGCCTCAGATCTGT+TGG	0.497915	8.4:+59052172	MS.gene60129:CDS
AAGTTATTATATCATATGGA+AGG	0.550124	8.4:+59052080	MS.gene60129:CDS
AATCAGTGCTTTATATGTAA+AGG	0.495574	8.4:-59051808	None:intergenic
AATTATAAGTAACAACACCA+GGG	0.736402	8.4:-59051590	None:intergenic
ACAAAGTGTCACAGATGTTC+TGG	0.267175	8.4:+59052270	MS.gene60129:CDS
ACAGATGTTCTGGGATCATG+TGG	0.636146	8.4:+59052280	MS.gene60129:CDS
ACAGCAGGTGCTTGATAAAA+TGG	0.279205	8.4:+59050876	MS.gene60129:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTAAGATGTTAGAAGC+TGG	+	chr8.4:59051771-59051790	MS.gene60129:CDS	30.0%
AAACATGAGAATTAACTTCC+GGG	-	chr8.4:59050963-59050982	None:intergenic	30.0%
ATGAACTCTTTGTTGAAAGA+TGG	+	chr8.4:59051099-59051118	MS.gene60129:CDS	30.0%
ATTATATCATATGGAAGGGA+AGG	+	chr8.4:59052085-59052104	MS.gene60129:CDS	30.0%
ATTGAACCCATTTGAAGAAA+TGG	-	chr8.4:59050774-59050793	None:intergenic	30.0%
CAATTATCGTAAAGGGTAAT+TGG	+	chr8.4:59050644-59050663	MS.gene60129:CDS	30.0%
CATACTAAACAATAGTTCCT+TGG	-	chr8.4:59051868-59051887	None:intergenic	30.0%
CATCTTCCATTTCTTCAAAT+GGG	+	chr8.4:59050765-59050784	MS.gene60129:CDS	30.0%
CTTGCAATAAGCATTTATCA+GGG	-	chr8.4:59051721-59051740	None:intergenic	30.0%
GAAGAAATGGAAGATGAAAA+AGG	-	chr8.4:59050761-59050780	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8.4	gene	59050619	59052361	59050619	ID=MS.gene60129
chr8.4	mRNA	59050619	59052361	59050619	ID=MS.gene60129.t1;Parent=MS.gene60129
chr8.4	exon	59050619	59052361	59050619	ID=MS.gene60129.t1.exon1;Parent=MS.gene60129.t1
chr8.4	CDS	59050619	59052361	59050619	ID=cds.MS.gene60129.t1;Parent=MS.gene60129.t1

Gene Sequence

>MS.gene60129

ATGATTTCGATTGAAAGTGTATGTTCAATTATCGTAAAGGGTAATTGGAACAATCTCTTAAAACCCAAAACAGCTTCTACACTCACTTCCACCACCATTCACCAAGTTATCTTGCACCTCACGCAACACCGTTACGAACCCTTTTTCATCTTCCATTTCTTCAAATGGGTTCAATCCATTCCACATTACACTCATTCCTTACAATCTTCATGGTCCATGATTCACATACTCACCAAACACCGCCATTTCAAAACTGCACAGCAGGTGCTTGATAAAATGGCGCAAAGGGAAATACTTTCATCGCCTTCGGTTTTGAGTTCTTTGGTGCGGATTCATGATGACCCGGAAGTTAATTCTCATGTTTTGAGCTGGATTGTGATACATTATGCGAAATCGAAGATGACCCATGATGCGGTTCAGGTTTTTGAGCAGATGAGTTTGTGTAATTTAAAGCCTCATTTGCATGCTTGTACTGTGCTTATGAACTCTTTGTTGAAAGATGGGGTTACTAGTATGGTGTGGAAGGTTTATAAGAGAATGATTCAAGATGGGGTTGTTCCGAATATTTATGTTTATAATTGTTTAATTCATGCTTGTTCGATATCGAGAGATGTGGAGAGGGCGGAGTTTATATTGAATGAGATGGAAGTGAAGGGTGTGGTTCCTGATATTTTTACGTACAATACTTTGATAGCTTTGTATTGCAAAAAGGGTATGCATTATGAGGCTTTATCGGTCCAGGATAAAATGGAAAGAGAGGGAATAAATCTTGATATTGTTAGTTATAATTCTCTTATTTATGGATTTTGCAAAGAAGGGAAAATGAGGGAAGCTATGAGGATGTTTGGCGAAATCAAAGATGCCATTCCGAACCATGTCACGTATACTACATTGATTGATGGATATTGTAAAGCGAATGAATTTGAGGAAGCTCTGAGGTTGCGTGAAATGATGGTGGCTAAGGGATTGTACCCTGGTGTTGTTACTTATAATTCGATTTTGCGCAAGCTGTGTTCAGATGGCAGGATTAGGGATGCAAACAAACTCTTGAACGAAATGAGTGAAAGAAAAGTCCAAGCTGACAGCATCACATGTAACACCCTGATAAATGCTTATTGCAAGATAGGAGATTTGAATTCTGCTTTGAAATTTAAAACTAAGATGTTAGAAGCTGGACTAACACCCAACTCCTTTACATATAAAGCACTGATTCATGGATTCTGCAAAACTAGTGAGCTCGAAAGTGCCAAGGAACTATTGTTTAGTATGCTTGATGCCGGATTTTCTCCAAATTATCGCACTTATTCGTGGATTGTAGATAGCTATTGCAAGAAAGACAATACTGATGCAGTTCTAGCACTGCCAGATGAGTTTTTGAGTAAAGGTTTTTGTCTTAATATTTCAGTCTACAGGGCATTGATAAGAAGGTTATGCAAGATAGAGAAGATTGAATGTGCTGAAAAGTTATTATATCATATGGAAGGGAAGGGTATTTCAGGTGATAGTGTTATCTACACTAGTCTCGCTTTTTCTTACTGGAAATCAGGGAACACAAATGCTGCCTCAGATCTGTTGGAAGAGATGGCTAGGAGAAGGTTGATGATCACTGTCAAAATCTATAGTTGCTTTAGTGCTCCTGATGCTAGTCAAAACAAAGTGTCACAGATGTTCTGGGATCATGTGGTGGAAAGGGGTCTGATGTCAAGAAATACAATGTATAAAATACAACAAATGCAGATATGA

Protein sequence

>MS.gene60129.t1

MISIESVCSIIVKGNWNNLLKPKTASTLTSTTIHQVILHLTQHRYEPFFIFHFFKWVQSIPHYTHSLQSSWSMIHILTKHRHFKTAQQVLDKMAQREILSSPSVLSSLVRIHDDPEVNSHVLSWIVIHYAKSKMTHDAVQVFEQMSLCNLKPHLHACTVLMNSLLKDGVTSMVWKVYKRMIQDGVVPNIYVYNCLIHACSISRDVERAEFILNEMEVKGVVPDIFTYNTLIALYCKKGMHYEALSVQDKMEREGINLDIVSYNSLIYGFCKEGKMREAMRMFGEIKDAIPNHVTYTTLIDGYCKANEFEEALRLREMMVAKGLYPGVVTYNSILRKLCSDGRIRDANKLLNEMSERKVQADSITCNTLINAYCKIGDLNSALKFKTKMLEAGLTPNSFTYKALIHGFCKTSELESAKELLFSMLDAGFSPNYRTYSWIVDSYCKKDNTDAVLALPDEFLSKGFCLNISVYRALIRRLCKIEKIECAEKLLYHMEGKGISGDSVIYTSLAFSYWKSGNTNAASDLLEEMARRRLMITVKIYSCFSAPDASQNKVSQMFWDHVVERGLMSRNTMYKIQQMQI