AlfalfaGEDB | MS.gene070228

M. sativa cultivar XinJiangDaYe / MS.gene070228

KEGG: -
GO: GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016620 GO:0016903 GO:0055114
PFAM: PF00171.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070228.t1	XP_003594746.1	100	595	0	0	1	595	1	595	0.00E+00	1195.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070228.t1	Q0WSF1	80.4	587	115	0	1	587	1	587	3.5e-287	988.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070228.t1	A2Q2X1	100.0	595	0	0	1	595	1	595	0.0e+00	1195.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058231	MS.gene070228	0.923033	4.52E-89	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene59503	MS.gene070228	PPI
MS.gene070228	MS.gene038584	PPI
MS.gene05525	MS.gene070228	PPI
MS.gene070228	MS.gene05926	PPI
MS.gene65609	MS.gene070228	PPI
MS.gene038961	MS.gene070228	PPI
MS.gene025244	MS.gene070228	PPI
MS.gene070228	MS.gene52096	PPI
MS.gene48392	MS.gene070228	PPI
MS.gene070228	MS.gene038583	PPI
MS.gene070228	MS.gene049130	PPI
MS.gene070228	MS.gene022281	PPI
MS.gene070228	MS.gene05924	PPI
MS.gene070228	MS.gene67483	PPI
MS.gene052441	MS.gene070228	PPI
MS.gene016597	MS.gene070228	PPI
MS.gene56963	MS.gene070228	PPI
MS.gene070228	MS.gene05526	PPI
MS.gene61417	MS.gene070228	PPI
MS.gene61421	MS.gene070228	PPI
MS.gene070228	MS.gene042955	PPI
MS.gene070228	MS.gene93333	PPI
MS.gene070228	MS.gene90955	PPI
MS.gene070228	MS.gene040757	PPI
MS.gene070228	MS.gene057729	PPI
MS.gene33139	MS.gene070228	PPI
MS.gene070228	MS.gene25940	PPI
MS.gene009119	MS.gene070228	PPI
MS.gene070228	MS.gene042286	PPI
MS.gene070228	MS.gene012582	PPI
MS.gene070228	MS.gene057392	PPI
MS.gene070228	MS.gene073734	PPI
MS.gene64804	MS.gene070228	PPI
MS.gene070228	MS.gene056242	PPI
MS.gene070228	MS.gene033206	PPI
MS.gene04769	MS.gene070228	PPI
MS.gene070228	MS.gene052440	PPI
MS.gene90958	MS.gene070228	PPI
MS.gene003502	MS.gene070228	PPI
MS.gene070228	MS.gene04770	PPI
MS.gene56929	MS.gene070228	PPI
MS.gene43277	MS.gene070228	PPI
MS.gene015744	MS.gene070228	PPI
MS.gene070228	MS.gene035576	PPI
MS.gene070228	MS.gene60353	PPI
MS.gene070228	MS.gene60330	PPI
MS.gene070228	MS.gene037163	PPI
MS.gene62087	MS.gene070228	PPI
MS.gene070228	MS.gene64463	PPI
MS.gene070228	MS.gene60129	PPI
MS.gene070228	MS.gene016051	PPI
MS.gene070228	MS.gene70611	PPI
MS.gene88489	MS.gene070228	PPI
MS.gene070228	MS.gene066203	PPI
MS.gene070228	MS.gene000715	PPI
MS.gene070228	MS.gene015742	PPI
MS.gene070228	MS.gene035486	PPI
MS.gene69883	MS.gene070228	PPI
MS.gene070228	MS.gene62486	PPI
MS.gene070228	MS.gene045715	PPI
MS.gene042956	MS.gene070228	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070228.t1	MTR_2g034160	100.000	595	0	0	1	595	1	595	0.0	1230
MS.gene070228.t1	MTR_2g067660	30.085	472	305	9	63	527	61	514	1.31e-62	215
MS.gene070228.t1	MTR_3g078170	30.655	473	308	9	62	528	75	533	3.43e-62	215
MS.gene070228.t1	MTR_4g106510	30.591	474	312	7	64	529	23	487	3.18e-60	208
MS.gene070228.t1	MTR_8g064170	30.078	512	319	11	26	528	51	532	7.65e-60	208
MS.gene070228.t1	MTR_6g086300	31.417	487	286	15	62	528	35	493	3.88e-59	206
MS.gene070228.t1	MTR_6g086300	31.417	487	286	15	62	528	35	493	3.88e-59	206
MS.gene070228.t1	MTR_8g083530	28.753	473	317	9	62	528	36	494	1.06e-58	204
MS.gene070228.t1	MTR_3g078550	29.958	474	313	9	64	528	23	486	1.97e-58	204
MS.gene070228.t1	MTR_3g078490	28.953	487	324	9	64	538	53	529	1.71e-57	202
MS.gene070228.t1	MTR_3g078550	30.435	460	301	9	78	528	7	456	9.41e-57	198
MS.gene070228.t1	MTR_4g107040	29.645	479	305	11	62	528	77	535	6.61e-56	198
MS.gene070228.t1	MTR_6g086340	28.964	473	314	10	62	527	33	490	2.61e-52	187
MS.gene070228.t1	MTR_8g064170	29.102	512	292	13	26	528	51	500	1.45e-51	185
MS.gene070228.t1	MTR_8g076480	27.778	468	308	8	87	540	8	459	7.27e-47	172
MS.gene070228.t1	MTR_4g131180	29.106	481	303	13	62	528	29	485	1.78e-46	171
MS.gene070228.t1	MTR_1g014320	28.513	491	313	13	52	528	19	485	6.74e-46	169
MS.gene070228.t1	MTR_3g078170	29.923	391	256	8	62	446	75	453	2.20e-45	167
MS.gene070228.t1	MTR_4g131180	29.252	441	275	12	62	488	29	446	9.03e-43	160
MS.gene070228.t1	MTR_8g083520	26.708	483	281	15	62	528	27	452	1.72e-42	159
MS.gene070228.t1	MTR_8g083520	30.641	359	195	8	180	528	58	372	6.53e-42	155
MS.gene070228.t1	MTR_1g029840	25.612	449	302	10	106	539	34	465	7.24e-41	155
MS.gene070228.t1	MTR_2g097950	26.829	451	299	10	96	535	35	465	1.63e-40	154
MS.gene070228.t1	MTR_8g094600	26.176	489	334	11	92	576	73	538	1.74e-40	155
MS.gene070228.t1	MTR_3g088150	25.390	449	303	10	106	539	36	467	1.98e-39	151
MS.gene070228.t1	MTR_1g029840	24.733	469	314	11	86	539	21	465	8.63e-39	149
MS.gene070228.t1	MTR_3g078170	29.268	369	243	8	62	424	75	431	4.42e-38	146
MS.gene070228.t1	MTR_3g078170	29.301	372	245	8	62	427	75	434	5.04e-38	146
MS.gene070228.t1	MTR_1314s0010	28.161	348	230	7	188	527	1	336	1.47e-35	137
MS.gene070228.t1	MTR_2g097950	25.727	447	274	10	96	535	35	430	2.31e-35	139
MS.gene070228.t1	MTR_8g083530	26.814	317	218	6	62	372	36	344	7.43e-29	118
MS.gene070228.t1	MTR_2g042330	23.617	470	335	8	56	516	27	481	2.40e-28	119
MS.gene070228.t1	MTR_1399s0020	33.043	230	136	4	318	547	136	347	2.50e-28	116
MS.gene070228.t1	MTR_3g078490	27.419	310	212	5	64	365	23	327	2.63e-28	117
MS.gene070228.t1	MTR_1222s0010	25.137	366	246	5	119	477	36	380	7.81e-28	119
MS.gene070228.t1	MTR_4g065040	28.916	415	268	11	110	514	27	424	3.02e-27	115
MS.gene070228.t1	MTR_1690s0010	26.686	341	219	9	62	388	22	345	8.95e-24	103
MS.gene070228.t1	MTR_4g093170	24.651	430	313	7	61	488	54	474	4.03e-23	103
MS.gene070228.t1	MTR_2g009750	24.533	428	305	10	66	488	31	445	7.14e-22	99.8
MS.gene070228.t1	MTR_2g009750	24.533	428	305	10	66	488	32	446	7.51e-22	99.8
MS.gene070228.t1	MTR_2g009750	24.299	428	307	9	66	488	32	447	8.05e-22	99.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene070228.t1	AT3G66658	80.272	588	116	0	1	588	1	588	0.0	1025
MS.gene070228.t1	AT3G66658	80.917	545	104	0	1	545	1	545	0.0	955
MS.gene070228.t1	AT1G74920	31.579	475	304	10	64	528	23	486	1.11e-62	215
MS.gene070228.t1	AT3G48170	30.738	488	312	13	64	537	23	498	1.70e-62	214
MS.gene070228.t1	AT1G23800	30.655	473	308	8	62	528	67	525	6.20e-61	211
MS.gene070228.t1	AT1G74920	32.192	438	281	9	96	528	55	481	1.81e-58	204
MS.gene070228.t1	AT3G48000	29.598	473	313	8	62	528	71	529	3.18e-58	204
MS.gene070228.t1	AT1G79440	28.511	470	313	8	64	527	68	520	1.75e-54	194
MS.gene070228.t1	AT3G24503	28.270	474	319	9	62	528	33	492	1.91e-54	193
MS.gene070228.t1	AT2G24270	29.253	482	301	13	62	528	29	485	4.56e-45	167
MS.gene070228.t1	AT2G24270	29.253	482	301	13	62	528	29	485	4.56e-45	167
MS.gene070228.t1	AT2G24270	29.132	484	303	13	60	528	27	485	5.20e-45	167
MS.gene070228.t1	AT2G24270	29.253	482	301	13	62	528	69	525	1.42e-44	166
MS.gene070228.t1	AT1G44170	25.050	499	341	10	87	578	11	483	4.42e-43	161
MS.gene070228.t1	AT1G44170	25.050	499	341	10	87	578	11	483	4.42e-43	161
MS.gene070228.t1	AT4G36250	26.196	439	297	8	86	514	6	427	2.68e-42	159
MS.gene070228.t1	AT1G44170	26.218	431	285	10	155	578	16	420	4.88e-40	151
MS.gene070228.t1	AT4G34240	25.740	439	297	9	82	514	76	491	5.23e-35	139
MS.gene070228.t1	AT4G34240	25.740	439	297	9	82	514	61	476	6.33e-35	139
MS.gene070228.t1	AT1G23800	29.688	320	205	6	62	372	67	375	9.33e-34	133
MS.gene070228.t1	AT4G34240	26.243	362	245	8	159	514	4	349	3.54e-31	126
MS.gene070228.t1	AT1G54100	23.077	468	334	10	57	514	27	478	1.33e-28	120
MS.gene070228.t1	AT1G54100	23.077	468	334	10	57	514	27	478	1.33e-28	120
MS.gene070228.t1	AT2G14170	24.027	437	307	9	61	488	17	437	3.12e-24	107
MS.gene070228.t1	AT2G14170	24.027	437	307	9	61	488	126	546	6.21e-24	106
MS.gene070228.t1	AT2G14170	24.027	437	307	9	61	488	126	546	6.51e-24	106
MS.gene070228.t1	AT4G34240	27.562	283	179	6	82	358	76	338	3.93e-18	87.0

Find 129 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCTAATGACTCAAAATA+TGG	0.189862	2.2:+54696870	MS.gene070228:CDS
GCCAGCCACCATGAAGTATT+TGG	0.227166	2.2:+54690988	MS.gene070228:CDS
TGGAAATGGAATTGTGATTA+AGG	0.231209	2.2:+54694408	MS.gene070228:CDS
TTGCAGGGCCCACCACTTGC+TGG	0.231865	2.2:+54696442	MS.gene070228:intron
TTCTTCCTTCAATGGCGTTT+TGG	0.273527	2.2:+54689555	None:intergenic
GTCAGGGCTGTTCTTCAGTC+AGG	0.294176	2.2:+54695519	MS.gene070228:CDS
TAGTATCCTGTGGCAGAAAA+TGG	0.307985	2.2:+54697619	MS.gene070228:intron
CAACATGTGAGAAGATAAAT+TGG	0.311009	2.2:+54693873	MS.gene070228:CDS
AGAAAATGGATTTGAATTTC+AGG	0.311412	2.2:+54697633	MS.gene070228:CDS
ATTGTTGCACGCGGAAGTTT+TGG	0.312303	2.2:+54696553	MS.gene070228:CDS
GGCGTAAAACACAGTGGATT+TGG	0.317784	2.2:+54697409	MS.gene070228:CDS
GAACATTCTGAAAAGCTTCT+AGG	0.321342	2.2:+54696496	MS.gene070228:CDS
CCACCAAGCTCTAGTGTAAC+TGG	0.324494	2.2:-54695363	None:intergenic
CTCTACAGGTGGCAATAAAA+AGG	0.324927	2.2:+54697744	MS.gene070228:CDS
CTTACCAGGTTTGGTGAAAC+AGG	0.328288	2.2:+54694846	MS.gene070228:intron
CGCTATATGGTCTTAGCATA+TGG	0.333976	2.2:+54697671	MS.gene070228:CDS
ACTTGCTGGGAAGTATGATA+TGG	0.337654	2.2:+54696456	MS.gene070228:CDS
TCAGAGCTAAACTTCATTAT+CGG	0.341800	2.2:-54696831	None:intergenic
GACCTTGTTGAGGTGATAAC+AGG	0.357161	2.2:+54694743	MS.gene070228:CDS
TCACTTGTTGAAGCGCTATA+TGG	0.360016	2.2:+54697658	MS.gene070228:CDS
GCATTTGACTTATAGGCATT+TGG	0.361428	2.2:+54696798	MS.gene070228:intron
TTTGGGATTTCTTCCTGCTT+TGG	0.373547	2.2:+54691006	MS.gene070228:CDS
TCCTGCTTTGGCGCCGGATG+AGG	0.376504	2.2:+54691018	MS.gene070228:CDS
GGTATCTTTGTCTTAATGAA+TGG	0.385200	2.2:-54697502	None:intergenic
CCAGCCACCATGAAGTATTT+GGG	0.385798	2.2:+54690989	MS.gene070228:CDS
AGGATATCTTCACGTGATAC+TGG	0.386025	2.2:+54693812	MS.gene070228:intron
AAGGGGAACGGCGCAGCAAT+CGG	0.391886	2.2:+54690943	MS.gene070228:CDS
TGCAGGGCCCACCACTTGCT+GGG	0.393682	2.2:+54696443	MS.gene070228:intron
CTATGGCAGCAAGGGCTGAT+TGG	0.398099	2.2:-54694709	None:intergenic
GTAACTTTGCTGACAAAAGA+TGG	0.410535	2.2:-54695594	None:intergenic
TCAGAACATGCAAGTTGGTC+TGG	0.413232	2.2:+54694665	MS.gene070228:CDS
AACGTCGATCGAAGGAACTT+TGG	0.413262	2.2:-54689642	None:intergenic
AGACAGACCTGAATAGGCTT+TGG	0.415353	2.2:-54697523	None:intergenic
AAAGATACCAAAGCCTATTC+AGG	0.419183	2.2:+54697516	MS.gene070228:CDS
ACGTCGATCGAAGGAACTTT+GGG	0.420608	2.2:-54689641	None:intergenic
ATTTCTTCCTGCTTTGGCGC+CGG	0.425705	2.2:+54691012	MS.gene070228:CDS
AAGAAATCCCAAATACTTCA+TGG	0.426395	2.2:-54690996	None:intergenic
GGACGATTCGGTGGTGTTGA+AGG	0.434305	2.2:+54697430	MS.gene070228:CDS
AGTCAGTAGTTGAAGATAGA+TGG	0.436417	2.2:+54697476	MS.gene070228:CDS
CATATATGAAATTATTCTCC+TGG	0.437895	2.2:-54689790	None:intergenic
CTTGCATCAATTTCATTGAG+TGG	0.441509	2.2:-54696636	None:intergenic
TGCATCGAATTACATGTGTC+AGG	0.444184	2.2:+54696983	MS.gene070228:CDS
CACAGTGGATTTGGACGATT+CGG	0.447805	2.2:+54697418	MS.gene070228:CDS
AACTTCATTATCGGCATGAT+AGG	0.455245	2.2:-54696822	None:intergenic
CTTCAACTACTGACTTTACA+AGG	0.459181	2.2:-54697468	None:intergenic
CCCAAATACTTCATGGTGGC+TGG	0.459911	2.2:-54690989	None:intergenic
AGGTTGCTCAAATTGCTGTC+AGG	0.459934	2.2:+54695502	MS.gene070228:intron
ATCACCTCAACAAGGTCCTC+TGG	0.461400	2.2:-54694737	None:intergenic
AAGGTCCTCTGGAGCTCCTA+TGG	0.461753	2.2:-54694726	None:intergenic
TTGCTAATGACTCAAAATAT+GGG	0.466403	2.2:+54696871	MS.gene070228:CDS
AGGGGAACGGCGCAGCAATC+GGG	0.468490	2.2:+54690944	MS.gene070228:CDS
ATGGGATCGTTTGCAAGCAT+TGG	0.469554	2.2:+54697690	MS.gene070228:CDS
CAAGGGCTGATTGGATGATC+CGG	0.470846	2.2:-54694700	None:intergenic
ATCACCTGGGGTTGGTAAGA+TGG	0.471554	2.2:+54694911	MS.gene070228:CDS
GATTGCTGCGCCGTTCCCCT+TGG	0.471929	2.2:-54690941	None:intergenic
TGCTGCGCCGTTCCCCTTGG+AGG	0.473549	2.2:-54690938	None:intergenic
AGCGAATGCAAGAACGAGAA+TGG	0.478429	2.2:-54689582	None:intergenic
GATAAATTGGCTATTGTCAG+AGG	0.478430	2.2:+54693886	MS.gene070228:CDS
TTAATTGCAGTGTTGGAAGT+TGG	0.483090	2.2:+54689758	MS.gene070228:intron
ACCTCATCCGGCGCCAAAGC+AGG	0.483725	2.2:-54691019	None:intergenic
TCAGGGCTGTTCTTCAGTCA+GGG	0.485988	2.2:+54695520	MS.gene070228:CDS
AAGATGGATAAATGTTCCTA+TGG	0.486775	2.2:-54695578	None:intergenic
CTGGAGCTCCTATGGCAGCA+AGG	0.488303	2.2:-54694718	None:intergenic
TAGCTTCTCATATACATTGT+GGG	0.490188	2.2:+54696940	MS.gene070228:CDS
CTTACTGAGCAGAACTCTAC+AGG	0.491012	2.2:+54697730	MS.gene070228:CDS
TGGAGCTCCTATGGCAGCAA+GGG	0.495946	2.2:-54694717	None:intergenic
ATAGGCTCACAAACCTCATC+CGG	0.501686	2.2:-54691031	None:intergenic
CAATCAGCCCTTGCTGCCAT+AGG	0.506439	2.2:+54694710	MS.gene070228:CDS
TTTGTTGGATCACCTGGGGT+TGG	0.506824	2.2:+54694903	MS.gene070228:CDS
GCTATATGGTCTTAGCATAT+GGG	0.521718	2.2:+54697672	MS.gene070228:CDS
ATATATGAAATTATTCTCCT+GGG	0.530434	2.2:-54689789	None:intergenic
CTTGCTGGGAAGTATGATAT+GGG	0.534705	2.2:+54696457	MS.gene070228:CDS
AGGGCTGTTCTTCAGTCAGG+GGG	0.535804	2.2:+54695522	MS.gene070228:CDS
GAAGTTTAGCTCTGATGAAG+AGG	0.535852	2.2:+54696839	MS.gene070228:CDS
GTAAATGATGCTTTAGACAA+AGG	0.536332	2.2:+54696523	MS.gene070228:CDS
TTTCCAGTCTCTGCCATTTG+GGG	0.540605	2.2:+54697387	MS.gene070228:intron
CAAAGACTAGCTTCAAGCAA+AGG	0.543389	2.2:+54691726	MS.gene070228:CDS
GGTTGCTCAAATTGCTGTCA+GGG	0.546154	2.2:+54695503	MS.gene070228:intron
TCACATTCACAATCACAGTA+GGG	0.555942	2.2:-54696612	None:intergenic
TCAGGTGCCTCCAAGGGGAA+CGG	0.558856	2.2:+54690931	MS.gene070228:intron
TATATGAGAAGCTATCTCTC+TGG	0.559110	2.2:-54696931	None:intergenic
TGGTGAAACAGGAGAAGCAC+TGG	0.562872	2.2:+54694857	MS.gene070228:CDS
AATGAAATTGATGCAAGAAG+AGG	0.563532	2.2:+54696642	MS.gene070228:CDS
ATACCAGTTACACTAGAGCT+TGG	0.564045	2.2:+54695360	MS.gene070228:CDS
AGAAGCACTGGTGTCTTCTG+TGG	0.565852	2.2:+54694869	MS.gene070228:CDS
CCAGTTACACTAGAGCTTGG+TGG	0.568636	2.2:+54695363	MS.gene070228:CDS
AATGTTTGAAACCTGAGTAC+AGG	0.570318	2.2:+54693915	MS.gene070228:CDS
ACAATGGTAGATGCTTCTCT+AGG	0.573876	2.2:+54693839	MS.gene070228:CDS
AGCTTCTCATATACATTGTG+GGG	0.574125	2.2:+54696941	MS.gene070228:CDS
CAGGGCTGTTCTTCAGTCAG+GGG	0.574303	2.2:+54695521	MS.gene070228:CDS
GAAGCATCAACGTCGATCGA+AGG	0.577147	2.2:-54689650	None:intergenic
GAACTTTGGGAGGAATGAGA+AGG	0.580699	2.2:-54689628	None:intergenic
TCATACTTCCCAGCAAGTGG+TGG	0.583531	2.2:-54696451	None:intergenic
TCATCTGTCCTAGTATCCTG+TGG	0.591006	2.2:+54697609	MS.gene070228:intron
TTCCAGTCTCTGCCATTTGG+GGG	0.595438	2.2:+54697388	MS.gene070228:intron
ATCGACGTTGATGCTTCCGA+TGG	0.598509	2.2:+54689656	MS.gene070228:CDS
TTATTGGGGCCATTGTGTCG+TGG	0.599690	2.2:+54694335	MS.gene070228:CDS
ATAGCTTCTCATATACATTG+TGG	0.604160	2.2:+54696939	MS.gene070228:CDS
TGAGACAGGTACCTGTACTC+AGG	0.604776	2.2:-54693926	None:intergenic
AGATATCAGAACATGCAAGT+TGG	0.612985	2.2:+54694660	MS.gene070228:intron
AATGGCCCCAATAACGCCAA+GGG	0.615997	2.2:-54694326	None:intergenic
GGAAGTTGGAAATCAATCCC+AGG	0.617637	2.2:+54689772	MS.gene070228:CDS
TGGGAGGAATGAGAAGGAGA+AGG	0.623716	2.2:-54689622	None:intergenic
CAATGGCCCCAATAACGCCA+AGG	0.623770	2.2:-54694327	None:intergenic
TGCTGCCATAGGAGCTCCAG+AGG	0.624048	2.2:+54694721	MS.gene070228:CDS
CATACCATCTTACCAACCCC+AGG	0.625175	2.2:-54694915	None:intergenic
AGTGGATTTGGACGATTCGG+TGG	0.625255	2.2:+54697421	MS.gene070228:CDS
AGCTCCAGAGGACCTTGTTG+AGG	0.625959	2.2:+54694733	MS.gene070228:CDS
ATATCATACTTCCCAGCAAG+TGG	0.627095	2.2:-54696454	None:intergenic
TGTTTGTGAAGACGTAGATG+TGG	0.627212	2.2:+54695401	MS.gene070228:CDS
TTCACATTCACAATCACAGT+AGG	0.630142	2.2:-54696613	None:intergenic
CACATTCACAATCACAGTAG+GGG	0.639355	2.2:-54696611	None:intergenic
GGGCTGTTCTTCAGTCAGGG+GGG	0.644321	2.2:+54695523	MS.gene070228:CDS
CATACTTCCCAGCAAGTGGT+GGG	0.644953	2.2:-54696450	None:intergenic
GGGATAGTTCCACGACACAA+TGG	0.651870	2.2:-54694344	None:intergenic
ATGAAGCATTGATCTTCCAG+AGG	0.652577	2.2:-54694278	None:intergenic
GCTTCATAAGAGAGCCAGAG+TGG	0.653340	2.2:+54694294	MS.gene070228:CDS
ACATTCACAATCACAGTAGG+GGG	0.657269	2.2:-54696610	None:intergenic
TTTATTCAGGTGCCTCCAAG+GGG	0.657310	2.2:+54690926	MS.gene070228:intron
ATAAATTGGCTATTGTCAGA+GGG	0.660827	2.2:+54693887	MS.gene070228:CDS
TCGATCGAAGGAACTTTGGG+AGG	0.662771	2.2:-54689638	None:intergenic
ACCTTGTTGAGGTGATAACA+GGG	0.667865	2.2:+54694744	MS.gene070228:CDS
CAGTAGTTGAAGATAGATGG+TGG	0.670419	2.2:+54697479	MS.gene070228:CDS
ATGGCCCCAATAACGCCAAG+GGG	0.691262	2.2:-54694325	None:intergenic
ACTGAGCAGAACTCTACAGG+TGG	0.704579	2.2:+54697733	MS.gene070228:CDS
GGAGCTGAAATTGTTGCACG+CGG	0.704741	2.2:+54696544	MS.gene070228:CDS
ACCCTGTTATCACCTCAACA+AGG	0.707587	2.2:-54694745	None:intergenic
AAATCCCAAATACTTCATGG+TGG	0.723996	2.2:-54690993	None:intergenic
TGGCCCCAATAACGCCAAGG+GGG	0.740602	2.2:-54694324	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	54689566	54697774	54689566	ID=MS.gene070228
chr2.2	mRNA	54689566	54697774	54689566	ID=MS.gene070228.t1;Parent=MS.gene070228
chr2.2	exon	54689566	54689677	54689566	ID=MS.gene070228.t1.exon1;Parent=MS.gene070228.t1
chr2.2	CDS	54689566	54689677	54689566	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54689768	54689811	54689768	ID=MS.gene070228.t1.exon2;Parent=MS.gene070228.t1
chr2.2	CDS	54689768	54689811	54689768	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54690935	54691039	54690935	ID=MS.gene070228.t1.exon3;Parent=MS.gene070228.t1
chr2.2	CDS	54690935	54691039	54690935	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54691680	54691807	54691680	ID=MS.gene070228.t1.exon4;Parent=MS.gene070228.t1
chr2.2	CDS	54691680	54691807	54691680	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54693814	54693936	54693814	ID=MS.gene070228.t1.exon5;Parent=MS.gene070228.t1
chr2.2	CDS	54693814	54693936	54693814	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54694276	54694429	54694276	ID=MS.gene070228.t1.exon6;Parent=MS.gene070228.t1
chr2.2	CDS	54694276	54694429	54694276	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54694662	54694765	54694662	ID=MS.gene070228.t1.exon7;Parent=MS.gene070228.t1
chr2.2	CDS	54694662	54694765	54694662	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54694854	54694932	54694854	ID=MS.gene070228.t1.exon8;Parent=MS.gene070228.t1
chr2.2	CDS	54694854	54694932	54694854	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54695333	54695428	54695333	ID=MS.gene070228.t1.exon9;Parent=MS.gene070228.t1
chr2.2	CDS	54695333	54695428	54695333	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54695504	54695641	54695504	ID=MS.gene070228.t1.exon10;Parent=MS.gene070228.t1
chr2.2	CDS	54695504	54695641	54695504	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54696448	54696663	54696448	ID=MS.gene070228.t1.exon11;Parent=MS.gene070228.t1
chr2.2	CDS	54696448	54696663	54696448	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54696813	54697004	54696813	ID=MS.gene070228.t1.exon12;Parent=MS.gene070228.t1
chr2.2	CDS	54696813	54697004	54696813	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54697394	54697537	54697394	ID=MS.gene070228.t1.exon13;Parent=MS.gene070228.t1
chr2.2	CDS	54697394	54697537	54697394	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1
chr2.2	exon	54697622	54697774	54697622	ID=MS.gene070228.t1.exon14;Parent=MS.gene070228.t1
chr2.2	CDS	54697622	54697774	54697622	ID=cds.MS.gene070228.t1;Parent=MS.gene070228.t1

Gene Sequence

>MS.gene070228

ATGGCGTTTTGGTGGTCCATTCTCGTTCTTGCATTCGCTTTCGCTATCTGCAAGTTCCTTCTCCTTCTCATTCCTCCCAAAGTTCCTTCGATCGACGTTGATGCTTCCGATGGTAATCTTCTTCGTTCATTCTTTTGCGTGTGAATTTGTTGTGAACTGTGAAAATGTAACGTTGATTTTATTTTTGTTTGATTAATTGCAGTGTTGGAAGTTGGAAATCAATCCCAGGAGAATAATTTCATATATGTGAGTAGTGATTTGATGCTTTTTTTTATTTATTTTGTTCATACATTTCTTTGATGCTTGGATAATTGAGATCTTGATGATGTGTTTAGTTTAATTTTGTTAGATCGATTTGGTTTTACTTTTGGATTTGAAATTTGAAATTAGAGTTCACTTTGATTGTAAATTGTGGTGACGAATTGATTTAATTTTATCTTGTGAAGCAACTGACACCATACATGACACGACACAGACACGAAACTGTCACTTGACACCCGTAAAAAAATTGAAAAAAATAAAGAATTTAATTAGTATACATGGTTAATGTACAATTATTTTACACATTCAATGATGTAAAATTGAAAAAAATTCTTAAGAACTTAGTTAGTAAACACCGTAAGTGTACAATTATTTTACACATGCATTCAATGATGTGCTGCCACTTCAATTAATAAATATTCATCATTGGATGCATGTGTAAAATAACTCATACCTGGTCGGTGCATAGTAATTAAACTCTTAATTAAATGTAATCGCATGTGTCAGTGTCTGACACTAACATGTCTGAGACACCGAACACGTCTTCTATCAGAAGTGACAGTGCTACAACTTATGGACTGTTTGGATTTGAGTTTATCTAGTGACAATGGCTTAGACACTTGTGAAACTACTGTTTGGATTGGCTTATTTTTTAGCTTATGCAAAACAGATTATGCAAATAAATAAGCTTTTATGTATTATTCATAAGCTTGTCAAGGTAGTTTATGAAAAAATAGCTTATGAAGATACAATTTTCGTTAACAAGAACTTATGAATTAACATAAAAGGTTATTTATTTGTATAAGCTATTTTTCATAAGCTCAAAAATAAGCCAATCCAAACGGACCGTTAGAGTTTATGGAAGCAGCTTATGACATGTCCATAAGTTTTTTGTAGCTTATTTTTGTAAGCTCTCCATAATAGCTTATGAAAACAACTTATAGCTAGCTTATATGAAAACAATTTGAATTATGCTATCTTTTATTATAGAAATAGCTTATACAAAGCGCTTAATTAAGCTGTTTATCCAAACAGGGCCTTAGCGTTAGTGGTTTGTAATTTTTTCCCCTTTTTGGAGAATCCAGTTGATCGATTGCTTTTTATTCAGGTGCCTCCAAGGGGAACGGCGCAGCAATCGGGCAAGAAAGTTCAGTGCTATGAGCCAGCCACCATGAAGTATTTGGGATTTCTTCCTGCTTTGGCGCCGGATGAGGTTTGTGAGCCTATGCTTATTGCTTCTCAAGAGACTTGTGATTGTGATGCATAATTTAACTAACTGAAAAGCAAACGGCCATGAAATATTTGGAATATGATGCATAGTATAGCTAAGATAGCAAGGAACTAGTAGTTTTAGATGCACAATGCCTATTTGAATGGAGTATTTTTTTTAATTGATAGTGTCAAAAGTTTACATAGTCTTCTAATCATAACTCACCAGGATGCCTCTGTTATATTACTTCTAAAATTATCCCATCAGATATCTACCCGGTGAGATATATGATAGGATGATTGTGTAAATTTTTTGATACCCTCTTAGCGCATAGTCATTAAACTCCTTTTTTATTTATTTAATGTTTATGCAAGTATTATATTTAGAAATAAGAAACCTCAAAATTTATACAGAGACAAAAAAATATCAAAGGGAATAAAACTAGTTGGGCTGAGTAGCTCGTGAGTTTGTTTGGTTCACTTGTATTTCTTATGAGTTATGCCAGCACTCAAGAAGCCATAAATATGAATAAGAACAAAGTTATTGCAAGAGTGTTCATAAAATATTATACCAGTTTGACTAATTTCATTCATGGGTTAATGTATTGCCATAGTTAACTTGTACTAATTTGAGATATGGATAGGTCAAGGAGCGAATTGAAAAAGTACGGAAGGCACAAAAAATGTGGGCAAAGACTAGCTTCAAGCAAAGGCGCCAGTTTTTACGTATACTACTGAAGTATATAATTAAACATCAAGCACTTATTTGCGAGTAAGACCACTTCAATTCCATGGTCATAGTTTGAAGATCAGGTGTTAGTTTTATTTTTAGGCTTAATTTCACTTTTGGCCCCCCAAGATTCACAATTGTGCAATTTTGCCGCCCCTCAAGTTTAAAATGAGTGATTTTGACTCCTTAAGTTTGCCTCCTTTTGCACTTACTAGTCCCCATGCACATTTTGACCAAAAAATGATGATGTGGCTAATTTCAATGACATGGCATATCTCTGTGTCCGCCAATGTGGAAATTAACATTTTTTTAAAAAAAATTTAAAAAAAGAGGAAATCAGTTTTATTTTAATTTTATTTCTTCTGAATTTTAATTAATTGTTTTCACACTCCCTCTTTATTTTAGTTTTACCAATAATTTTCCTTCTTAATTTTCACTCTAGTTTCAGTTTCCCCCTTCCCTTCTTTCAAGTTTGGAGCCAATAACCATAAAGTCTCAACTTAATCGTCTATACATACTGCAGAAAATGTGTGAACCAAACTAATACTCAATCATACTAGTCAAAAAACTCAAATTATCCACAAAAATGCAGCGTTGATAAAAACACGCATGTATCCCCCGTGAGCTTAGCTCAGTTAGTAGGGATAATGCATAATATATGCAAGGTCTGGGGTTCGAATCCCAGACACCACCAAAAAAAGATAAAACACGCATGCAACATGTATATCTAAGTGTTTGATTCTACTGTTTAACACAATTGTATCATATCCTACTTATAACATATTTCAATATATCACAATGTACTAGTGGATAAGCAATTAGATATATTAGCAGATAGGAGGCTTGGTTGGAGGTGGGACTGGGGGTGCTATCTATGGAAGAAATGGGGTGCTCTGTAGAGTAGTTCATATGTGAAAAAATTAATTAAAATTGAGCAGAAATATATATAAAAAAAAAAAAAAAAAGTATCATTAAGAGTTCAATCTGAAAAAGAAAAAAAAAAAAAAACTGATTTCTTTCTTTTTTTTTAAATGTTAATTTCAACATTGGCGAACACATGGAAATGCCACGTCATGACAATTAACCACGTTATTATTTTTTGGTCAAAATATGCATGGGGGACTAGTAAGGGTAAAAGCTCTGAAGCACGGGTACTCCGTTTTGCTAAACGTACCCGTACCGGGTACTGCGCGGGTACTGGTACACGTACGGTACGCGTACTGTACGCACCCATGAAGCACCAGGTTTTTTTTTTTTTTTTTAATGGTTGGGTACGGCCGGGGTACTGCAGTGGTACTGATTGGGTACGTTTATCCTCTTTTTTTGGAGAAAATGGAGCAATTATAGTTTTTTTTTGTTTTTTTATAAAAGTCAAACTTAACTCCACGCATATCCTCTTTTTTTTTTTTGAGAAAAACAAGCCCTTTTATGAGAATTAATAGCATTGAAGCTAGTCATTTTACTGAAGATGAGGAAGGAAATAGTGATATCGATAGAGTTGGAGTGTGATTTTTCCTATTCATGCATAGTTATTAAGTTTTATCCGGTACTTTAGTTTTTCAATTTGGATGTTTTAGCGTTGTAATTTTTTTTTTTTGGTTGAATTTTTGTCGTCATTTGAGCAATATAATTTTCTCATGCAATGTTATTTATTTTAAATTAAAAAAAAAAAAAATTATCGTAACGTACCCGTACCTTTGTTTTTTGAGAAATGCCGTATCCCGTACCCGCACCCGCACCCGTACCGGGTACCGTACCCGCACCCGTGCAACACAGGGTAAAAGGGGCTAAAATCACTCATTTTAAACTTGGGGGACCAAAATCGCACAATTGTGAAACTTTAGGGGCCAAAATGCAATTAAGCCTTATTTTTATTTTTATTTTTAAAGAGAAAACTTATTTAATGTTGCATTGTGAGTTGTGACATCCTGGCGAACATGCAGTTTTTAGCATAGTTTTAATTGTTAGTCTGAAAAATGGATTTGAACTGGGCTTGTTGCATAAGAATATCAATATGAGATAAATTCTAACTTGTGTCCTTTTATTAGGATATCTTCACGTGATACTGGAAAAACAATGGTAGATGCTTCTCTAGGAGAAATAATGACAACATGTGAGAAGATAAATTGGCTATTGTCAGAGGGTGAAAAATGTTTGAAACCTGAGTACAGGTACCTGTCTCAATCTTTTTTGTGTTTTACTAATAATTTTATCATCTTACTTTAGACTGAAAACACTAGTAGTTCTCTGCATGTCTTTATTTATTCAAAAGAAAACAATACCCCCTCCCACAATCCCAGTAATTGACTCTTTTCTCTGCTTAAATTGACTTATGTTAAATTGTCTATCATTTTATTTTCTTTGTTCTTTACTGTATGCTTTCTTTTGCTTCCTACAGTTTTTTTTTTCTTTTTTCATGTAAATAAAGAATGTTTCTGTGTTAATATCGCAAATTAGCTAATTCACTTTCATGACCACCTTTTGTTTTGCAATACATTAAAAACATGCAGATCCTCTGGAAGATCAATGCTTCATAAGAGAGCCAGAGTGGAATTTCACCCCCTTGGCGTTATTGGGGCCATTGTGTCGTGGAACTATCCCTTCCATAATATTTTTAACCCTATGCTAGCAGCAGTTTTTTCTGGAAATGGAATTGTGATTAAGGTATAAAATGACTTAATATGTACTGTGTATACCTTCTCTAAAATTGTTCATCATATGGATGGATTACCATGTTTTTATGAAAGTAGATATTGTTAACTGTCAGGCAGCCTGACAGTTAACAATGTTCCATTAACACTTGATACTAAAAATTGAGACTCTGCAACATGCAACACCTACTCCTCTGTGTTTATATTTTTCAGTTTGACAGAAATCCTTGTGATTCTGATTGTAGATATCAGAACATGCAAGTTGGTCTGGATGCTTTTACTTCCGGATCATCCAATCAGCCCTTGCTGCCATAGGAGCTCCAGAGGACCTTGTTGAGGTGATAACAGGGTAGAGCCTTATTTTGCACCCTCCCTTTTTTCCTTTAATTTCAAAGTCATGTCTGCATCTATAATCTCAATTGGACCTTTCTTACCAGGTTTGGTGAAACAGGAGAAGCACTGGTGTCTTCTGTGGATAAAGTCATTTTTGTTGGATCACCTGGGGTTGGTAAGATGGTATGGTTCCTTAAGCCAATCAAATTGGTTCATCCTGTCCTGTCTTCTGTGAATTGCATCACATAGTAGTTTTATTTATGATTCTTAAAATATAGTTTATGATATGTTGTGCATCATATATGTTTAGTAAAGAATTTTCCTAGAATACTTGGTCAATGTTTAGAGTAGCATTTGGAGCAGCTGAATCCCCATTTTGTACTCACAAAAGAAAAGAGTAACGTCAAAAGTTGTAGTATACCTCTAGTCTAAACAGCGACGTCCGTTAAGCAAAATCAGTGAGTGAGATAGTTTGGGGCTTTTTGTCTACTTTTCCGTATATGCTTCTTCTATTCTTTCCGAGATGTTCCATGCAATGAGTAGTCTTTCGCATGTTGATAATATCATTTGTTCTTTTATGCAGATAATGAAAAATGCTTCTGAGACACTTATACCAGTTACACTAGAGCTTGGTGGAAAAGATGCATTTATTGTTTGTGAAGACGTAGATGTGGACCATGTATGAATACCTTCCCCATTATGCATTCGTTGTTGAATTCCATAATTTTAACACTCACTAATCTGCTGAATGTAGGTTGCTCAAATTGCTGTCAGGGCTGTTCTTCAGTCAGGGGGGCAGAACTGTGCTGGTGCTGAACGATTTTATGTCCATAGGAACATTTATCCATCTTTTGTCAGCAAAGTTACCAAAATCATAAAATCTGTTACAGCTGTAAGTGTTTATGCAAGTGTTTTTCCATGACCTTTCATTTGTAACATCTGTCGTGTGCAATTTCTTTCCAAGTCAACATGGGCTTCTTGTCACATTTGCCTGCACTTGATTCTTACAGAATTGCTATATCACATGTCCACCAGCTTAACTTTACATTGGTTCTAGTTTATTATGAAATTTTGCAAGACGGTTGTATGATTCATGATATGTTGTTTTTCCTCTATTTTTGTTGCATTTCTGCATGTCTCGTTTTCTATTTTCCTTAGAGGAGGGAAGAGAAAGAAATAAAGAGTCTGAGTTATGATGATGATTTTATACAATTTACTAAACACGACCCTTATTTATATTTATACTGAACTTCTAATCCTAATTAAATAAGGAAAAATATCATGAAATCTAAAGAGATAGAGAAACCAATCTTCAGAGATAATCTATGATATTCTCAATTACTTTAGCACAACTCAGTATTATGTGTTCTTTGTTTTTTAATGTTATGACATAGATTGTTATTTATTAGACTGAAGTGTTAATATATGGCAAATTGTGAAATGATTGTTATTCTTCAAATAACTAATGCTGAGAAGTTGGGATCTATGATTAGATAATAGATATACTGATGAGAGGAAGGGACAAAAATGTCATGAAGTTGTATAAATCCCACAGATTATATTAACATTGAAAACTGAGGACTTCTGTATGAGCAAGAACTATATTTTCACTTATTAATGGAGCATAGGGGTCTTCCGCAAAATATTTAGTATTAAGAAGTCCGTTCTTTAAAGCTTTTGGTTAATGATTTCTTGCAGGGCCCACCACTTGCTGGGAAGTATGATATGGGAGCTTTATGCATGCATGAACATTCTGAAAAGCTTCTAGGTCTTGTAAATGATGCTTTAGACAAAGGAGCTGAAATTGTTGCACGCGGAAGTTTTGGACATATAGGCGAAGATGCTGTTGATCAGTATTTTCCCCCTACTGTGATTGTGAATGTGAACCACTCAATGAAATTGATGCAAGAAGAGGTAGCAAACCTTTACTACAAGCATTGGATATTTTGAAATTTGAAAAACTGATTCTCATGTAGTGCAACCAACTATTATTTGAAAATGTCTTCCTATCTTGTATGCTCCAACAACTGCTTACTAGATTCATCTGTGCATTTGACTTATAGGCATTTGGACCTATCATGCCGATAATGAAGTTTAGCTCTGATGAAGAGGTTATCAAGCTTGCTAATGACTCAAAATATGGGCTCAGCTGTGCTGTTTTCTCAGGCAGTCAAAGTCATGCCAGAGAGATAGCTTCTCATATACATTGTGGGGTTGCTGCAGTTAATGATTTTGCATCGAATTACATGTGTCAGGTAAAATTTGATTTTAAATAGTTTATCTCTCGCTCCATGTTTGCGTGTCACCTGAAAGGGACCTAGTAGTAGCGTGATATGATGTGTGCTTTGTTGATATCATCCATCGCTAAAGAACAAGAGAAACAACTAAAATATTAGGAGCATGGTTGTGAAATTCAGACTAGACTGGCCAATCTAACGTTTTGTTCACAGTCCTATAGAACTAGCTTGAACCGGATAGAATCATGGTTCGAATGCAATTGGTTCAGTCGGTCATTATTATTTTTTTTTTAATTAACAAAATTTTGTACATTTTGTTTTAGTACTGTCGGTATATGTTCCTAATTCAACCTGCGTGTACCTTTTTAAATTTCCATTGAAGCAAAGTTGCATTCTGATTTTTCCAGTCTCTGCCATTTGGGGGCGTAAAACACAGTGGATTTGGACGATTCGGTGGTGTTGAAGGCTTGCGAGCATGCTGCCTTGTAAAGTCAGTAGTTGAAGATAGATGGTGGCCATTCATTAAGACAAAGATACCAAAGCCTATTCAGGTCTGTCTAAAAAACCTTTCATTATATCATACAAGCTACACTGTTGGTGGATAGATACTGAGATGCACTTTTCATCTGTCCTAGTATCCTGTGGCAGAAAATGGATTTGAATTTCAGGAGTCACTTGTTGAAGCGCTATATGGTCTTAGCATATGGGATCGTTTGCAAGCATTGGTAAACGTTTTGAAAATGCTTACTGAGCAGAACTCTACAGGTGGCAATAAAAAGGAGAAATGA

Protein sequence

>MS.gene070228.t1

MAFWWSILVLAFAFAICKFLLLLIPPKVPSIDVDASDVLEVGNQSQENNFIYVPPRGTAQQSGKKVQCYEPATMKYLGFLPALAPDEVKERIEKVRKAQKMWAKTSFKQRRQFLRILLKYIIKHQALICEISSRDTGKTMVDASLGEIMTTCEKINWLLSEGEKCLKPEYRSSGRSMLHKRARVEFHPLGVIGAIVSWNYPFHNIFNPMLAAVFSGNGIVIKISEHASWSGCFYFRIIQSALAAIGAPEDLVEVITGFGETGEALVSSVDKVIFVGSPGVGKMIMKNASETLIPVTLELGGKDAFIVCEDVDVDHVAQIAVRAVLQSGGQNCAGAERFYVHRNIYPSFVSKVTKIIKSVTAGPPLAGKYDMGALCMHEHSEKLLGLVNDALDKGAEIVARGSFGHIGEDAVDQYFPPTVIVNVNHSMKLMQEEAFGPIMPIMKFSSDEEVIKLANDSKYGLSCAVFSGSQSHAREIASHIHCGVAAVNDFASNYMCQSLPFGGVKHSGFGRFGGVEGLRACCLVKSVVEDRWWPFIKTKIPKPIQYPVAENGFEFQESLVEALYGLSIWDRLQALVNVLKMLTEQNSTGGNKKEK