Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60166.t1 | RHN63082.1 | 98.6 | 213 | 3 | 0 | 1 | 213 | 81 | 293 | 1.50E-127 | 465.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60166.t1 | Q8LER3 | 78.4 | 213 | 46 | 0 | 1 | 213 | 81 | 293 | 7.4e-107 | 387.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60166.t1 | G7JF94 | 98.6 | 213 | 3 | 0 | 1 | 213 | 79 | 291 | 1.1e-127 | 465.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene60166 | 0.80847 | 3.05E-50 | -1.69E-46 |
| MS.gene050122 | MS.gene60166 | 0.805751 | 1.15E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene60166 | 0.822535 | 2.25E-53 | -1.69E-46 |
| MS.gene050926 | MS.gene60166 | -0.807484 | 4.94E-50 | -1.69E-46 |
| MS.gene051387 | MS.gene60166 | 0.808093 | 3.67E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene60166 | -0.800556 | 1.36E-48 | -1.69E-46 |
| MS.gene052253 | MS.gene60166 | 0.802035 | 6.79E-49 | -1.69E-46 |
| MS.gene053301 | MS.gene60166 | 0.811124 | 8.19E-51 | -1.69E-46 |
| MS.gene053813 | MS.gene60166 | 0.817089 | 3.95E-52 | -1.69E-46 |
| MS.gene054183 | MS.gene60166 | 0.804687 | 1.92E-49 | -1.69E-46 |
| MS.gene054435 | MS.gene60166 | -0.809768 | 1.61E-50 | -1.69E-46 |
| MS.gene054437 | MS.gene60166 | -0.813889 | 2.04E-51 | -1.69E-46 |
| MS.gene054447 | MS.gene60166 | -0.818403 | 2.00E-52 | -1.69E-46 |
| MS.gene054486 | MS.gene60166 | 0.842177 | 3.03E-58 | -1.69E-46 |
| MS.gene055486 | MS.gene60166 | 0.809917 | 1.49E-50 | -1.69E-46 |
| MS.gene055909 | MS.gene60166 | 0.841938 | 3.51E-58 | -1.69E-46 |
| MS.gene055911 | MS.gene60166 | 0.843256 | 1.57E-58 | -1.69E-46 |
| MS.gene05601 | MS.gene60166 | 0.803123 | 4.05E-49 | -1.69E-46 |
| MS.gene056448 | MS.gene60166 | 0.811348 | 7.32E-51 | -1.69E-46 |
| MS.gene056511 | MS.gene60166 | 0.823621 | 1.25E-53 | -1.69E-46 |
| MS.gene057146 | MS.gene60166 | 0.802433 | 5.62E-49 | -1.69E-46 |
| MS.gene057469 | MS.gene60166 | 0.80084 | 1.19E-48 | -1.69E-46 |
| MS.gene057823 | MS.gene60166 | 0.80079 | 1.22E-48 | -1.69E-46 |
| MS.gene057888 | MS.gene60166 | 0.802851 | 4.61E-49 | -1.69E-46 |
| MS.gene059368 | MS.gene60166 | 0.809361 | 1.97E-50 | -1.69E-46 |
| MS.gene059420 | MS.gene60166 | 0.831384 | 1.72E-55 | -1.69E-46 |
| MS.gene059462 | MS.gene60166 | 0.809438 | 1.89E-50 | -1.69E-46 |
| MS.gene059711 | MS.gene60166 | 0.838524 | 2.73E-57 | -1.69E-46 |
| MS.gene059876 | MS.gene60166 | 0.805685 | 1.18E-49 | -1.69E-46 |
| MS.gene060837 | MS.gene60166 | 0.855059 | 8.13E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60166.t1 | MTR_4g097700 | 98.592 | 213 | 3 | 0 | 1 | 213 | 116 | 328 | 5.17e-162 | 450 |
| MS.gene60166.t1 | MTR_4g097640 | 98.592 | 213 | 3 | 0 | 1 | 213 | 79 | 291 | 1.47e-161 | 447 |
| MS.gene60166.t1 | MTR_4g097670 | 98.592 | 213 | 3 | 0 | 1 | 213 | 79 | 291 | 1.47e-161 | 447 |
| MS.gene60166.t1 | MTR_7g084770 | 57.143 | 210 | 88 | 2 | 1 | 209 | 79 | 287 | 1.23e-88 | 263 |
| MS.gene60166.t1 | MTR_7g084760 | 57.143 | 210 | 88 | 2 | 1 | 209 | 79 | 287 | 1.70e-88 | 263 |
| MS.gene60166.t1 | MTR_2g089140 | 54.795 | 219 | 89 | 2 | 1 | 210 | 7 | 224 | 8.98e-88 | 258 |
| MS.gene60166.t1 | MTR_6g033085 | 57.619 | 210 | 87 | 2 | 1 | 209 | 79 | 287 | 1.76e-87 | 260 |
| MS.gene60166.t1 | MTR_2g089140 | 54.795 | 219 | 89 | 2 | 1 | 210 | 70 | 287 | 2.79e-87 | 259 |
| MS.gene60166.t1 | MTR_6g033055 | 56.667 | 210 | 89 | 2 | 1 | 209 | 79 | 287 | 3.58e-87 | 259 |
| MS.gene60166.t1 | MTR_7g084750 | 55.238 | 210 | 92 | 2 | 1 | 209 | 79 | 287 | 1.32e-86 | 258 |
| MS.gene60166.t1 | MTR_4g045853 | 53.881 | 219 | 91 | 3 | 1 | 210 | 72 | 289 | 3.97e-85 | 254 |
| MS.gene60166.t1 | MTR_1g105495 | 56.338 | 213 | 89 | 2 | 1 | 209 | 82 | 294 | 6.04e-85 | 254 |
| MS.gene60166.t1 | MTR_1g105900 | 54.245 | 212 | 93 | 4 | 1 | 209 | 81 | 291 | 4.17e-83 | 249 |
| MS.gene60166.t1 | MTR_8g102910 | 54.419 | 215 | 92 | 4 | 1 | 210 | 67 | 280 | 2.86e-79 | 239 |
| MS.gene60166.t1 | MTR_4g128410 | 53.521 | 213 | 91 | 5 | 1 | 209 | 72 | 280 | 1.71e-78 | 237 |
| MS.gene60166.t1 | MTR_4g126920 | 52.336 | 214 | 93 | 4 | 1 | 209 | 72 | 281 | 3.64e-77 | 233 |
| MS.gene60166.t1 | MTR_8g013055 | 53.521 | 213 | 92 | 5 | 1 | 210 | 78 | 286 | 4.56e-77 | 233 |
| MS.gene60166.t1 | MTR_6g088320 | 52.778 | 216 | 96 | 4 | 2 | 212 | 72 | 286 | 8.65e-77 | 233 |
| MS.gene60166.t1 | MTR_4g128400 | 51.185 | 211 | 101 | 2 | 1 | 210 | 75 | 284 | 2.20e-76 | 231 |
| MS.gene60166.t1 | MTR_7g093530 | 49.767 | 215 | 98 | 4 | 1 | 210 | 71 | 280 | 2.09e-74 | 226 |
| MS.gene60166.t1 | MTR_4g128580 | 52.804 | 214 | 95 | 4 | 1 | 209 | 73 | 285 | 1.88e-73 | 224 |
| MS.gene60166.t1 | MTR_4g128570 | 51.429 | 210 | 86 | 4 | 1 | 209 | 78 | 272 | 1.05e-72 | 222 |
| MS.gene60166.t1 | MTR_4g128590 | 48.624 | 218 | 99 | 4 | 1 | 209 | 62 | 275 | 2.86e-72 | 221 |
| MS.gene60166.t1 | MTR_8g081040 | 50.952 | 210 | 96 | 4 | 1 | 209 | 78 | 281 | 3.89e-71 | 218 |
| MS.gene60166.t1 | MTR_3g084630 | 50.000 | 210 | 97 | 3 | 1 | 209 | 75 | 277 | 7.10e-71 | 217 |
| MS.gene60166.t1 | MTR_3g084620 | 50.000 | 210 | 97 | 3 | 1 | 209 | 75 | 277 | 8.64e-71 | 217 |
| MS.gene60166.t1 | MTR_2g038860 | 47.273 | 220 | 103 | 4 | 1 | 211 | 73 | 288 | 3.49e-70 | 216 |
| MS.gene60166.t1 | MTR_2g038705 | 47.273 | 220 | 103 | 4 | 1 | 211 | 73 | 288 | 3.49e-70 | 216 |
| MS.gene60166.t1 | MTR_7g056090 | 48.020 | 202 | 100 | 4 | 1 | 201 | 79 | 276 | 8.48e-63 | 197 |
| MS.gene60166.t1 | MTR_5g091730 | 45.238 | 210 | 108 | 3 | 1 | 209 | 75 | 278 | 4.37e-62 | 195 |
| MS.gene60166.t1 | MTR_8g102220 | 45.238 | 210 | 95 | 4 | 1 | 209 | 76 | 266 | 2.64e-60 | 190 |
| MS.gene60166.t1 | MTR_3g089540 | 46.919 | 211 | 105 | 4 | 1 | 209 | 77 | 282 | 6.13e-59 | 187 |
| MS.gene60166.t1 | MTR_2g049030 | 42.439 | 205 | 109 | 6 | 1 | 203 | 77 | 274 | 5.09e-55 | 177 |
| MS.gene60166.t1 | MTR_2g049130 | 44.670 | 197 | 104 | 5 | 1 | 196 | 76 | 268 | 1.92e-53 | 173 |
| MS.gene60166.t1 | MTR_5g029100 | 41.204 | 216 | 117 | 5 | 3 | 209 | 60 | 274 | 2.82e-50 | 166 |
| MS.gene60166.t1 | MTR_5g029100 | 41.204 | 216 | 117 | 5 | 3 | 209 | 91 | 305 | 1.58e-49 | 164 |
| MS.gene60166.t1 | MTR_1g033420 | 42.254 | 213 | 116 | 6 | 3 | 210 | 82 | 292 | 4.49e-49 | 163 |
| MS.gene60166.t1 | MTR_4g057450 | 38.278 | 209 | 110 | 5 | 3 | 201 | 97 | 296 | 6.63e-47 | 157 |
| MS.gene60166.t1 | MTR_2g095800 | 38.073 | 218 | 115 | 7 | 3 | 209 | 101 | 309 | 1.45e-46 | 156 |
| MS.gene60166.t1 | MTR_7g102260 | 38.991 | 218 | 112 | 7 | 3 | 209 | 84 | 291 | 2.73e-45 | 152 |
| MS.gene60166.t1 | MTR_8g090100 | 37.799 | 209 | 117 | 5 | 10 | 210 | 79 | 282 | 2.10e-44 | 150 |
| MS.gene60166.t1 | MTR_1g063020 | 38.182 | 220 | 111 | 7 | 3 | 209 | 87 | 294 | 4.91e-44 | 149 |
| MS.gene60166.t1 | MTR_8g064180 | 35.780 | 218 | 127 | 6 | 3 | 209 | 95 | 310 | 1.30e-42 | 145 |
| MS.gene60166.t1 | MTR_8g479340 | 37.297 | 185 | 54 | 3 | 27 | 209 | 11 | 135 | 6.45e-33 | 115 |
| MS.gene60166.t1 | MTR_8g032640 | 32.432 | 148 | 93 | 4 | 2 | 148 | 59 | 200 | 5.89e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene60166.t1 | AT4G37800 | 78.404 | 213 | 46 | 0 | 1 | 213 | 81 | 293 | 7.32e-132 | 372 |
| MS.gene60166.t1 | AT5G65730 | 79.904 | 209 | 42 | 0 | 1 | 209 | 82 | 290 | 8.90e-132 | 372 |
| MS.gene60166.t1 | AT4G03210 | 59.434 | 212 | 82 | 2 | 1 | 209 | 46 | 256 | 4.12e-90 | 265 |
| MS.gene60166.t1 | AT5G13870 | 57.143 | 210 | 88 | 2 | 1 | 209 | 79 | 287 | 1.47e-89 | 265 |
| MS.gene60166.t1 | AT5G13870 | 57.143 | 210 | 88 | 2 | 1 | 209 | 79 | 287 | 1.47e-89 | 265 |
| MS.gene60166.t1 | AT5G13870 | 57.143 | 210 | 88 | 2 | 1 | 209 | 79 | 287 | 1.47e-89 | 265 |
| MS.gene60166.t1 | AT4G03210 | 59.434 | 212 | 82 | 2 | 1 | 209 | 74 | 284 | 1.71e-89 | 265 |
| MS.gene60166.t1 | AT2G06850 | 56.190 | 210 | 90 | 2 | 1 | 209 | 22 | 230 | 9.72e-86 | 253 |
| MS.gene60166.t1 | AT2G06850 | 56.190 | 210 | 90 | 2 | 1 | 209 | 82 | 290 | 3.65e-85 | 254 |
| MS.gene60166.t1 | AT1G11545 | 55.300 | 217 | 87 | 4 | 1 | 209 | 85 | 299 | 3.30e-84 | 252 |
| MS.gene60166.t1 | AT2G14620 | 53.302 | 212 | 95 | 3 | 1 | 209 | 34 | 244 | 4.46e-83 | 247 |
| MS.gene60166.t1 | AT2G14620 | 53.302 | 212 | 95 | 3 | 1 | 209 | 23 | 233 | 6.68e-83 | 246 |
| MS.gene60166.t1 | AT2G14620 | 53.302 | 212 | 95 | 3 | 1 | 209 | 84 | 294 | 9.64e-83 | 248 |
| MS.gene60166.t1 | AT5G57550 | 53.555 | 211 | 91 | 4 | 1 | 209 | 77 | 282 | 6.21e-79 | 238 |
| MS.gene60166.t1 | AT3G23730 | 52.336 | 214 | 96 | 4 | 1 | 209 | 74 | 286 | 1.56e-77 | 234 |
| MS.gene60166.t1 | AT4G14130 | 51.174 | 213 | 97 | 4 | 1 | 209 | 75 | 284 | 1.29e-75 | 229 |
| MS.gene60166.t1 | AT5G57560 | 49.772 | 219 | 97 | 4 | 1 | 211 | 70 | 283 | 2.85e-75 | 229 |
| MS.gene60166.t1 | AT5G57530 | 51.402 | 214 | 94 | 5 | 1 | 209 | 74 | 282 | 2.88e-75 | 229 |
| MS.gene60166.t1 | AT4G25810 | 51.376 | 218 | 94 | 5 | 1 | 211 | 73 | 285 | 4.84e-75 | 228 |
| MS.gene60166.t1 | AT5G48070 | 48.815 | 211 | 100 | 5 | 1 | 209 | 77 | 281 | 1.60e-74 | 226 |
| MS.gene60166.t1 | AT4G30290 | 49.763 | 211 | 98 | 5 | 1 | 209 | 72 | 276 | 4.48e-74 | 225 |
| MS.gene60166.t1 | AT5G57540 | 50.467 | 214 | 96 | 5 | 1 | 209 | 73 | 281 | 1.82e-73 | 224 |
| MS.gene60166.t1 | AT4G13090 | 50.943 | 212 | 99 | 3 | 1 | 209 | 79 | 288 | 3.06e-73 | 224 |
| MS.gene60166.t1 | AT4G30280 | 49.289 | 211 | 99 | 5 | 1 | 209 | 77 | 281 | 4.05e-73 | 223 |
| MS.gene60166.t1 | AT1G65310 | 49.763 | 211 | 98 | 5 | 1 | 209 | 77 | 281 | 3.24e-72 | 221 |
| MS.gene60166.t1 | AT1G65310 | 49.763 | 211 | 98 | 5 | 1 | 209 | 99 | 303 | 4.22e-72 | 221 |
| MS.gene60166.t1 | AT4G30270 | 49.528 | 212 | 91 | 4 | 1 | 211 | 71 | 267 | 4.40e-72 | 220 |
| MS.gene60166.t1 | AT4G25820 | 50.000 | 214 | 97 | 4 | 1 | 209 | 77 | 285 | 4.18e-71 | 218 |
| MS.gene60166.t1 | AT4G28850 | 47.442 | 215 | 106 | 3 | 1 | 209 | 74 | 287 | 1.10e-68 | 212 |
| MS.gene60166.t1 | AT4G13080 | 47.847 | 209 | 106 | 3 | 1 | 209 | 86 | 291 | 2.50e-68 | 211 |
| MS.gene60166.t1 | AT3G25050 | 47.170 | 212 | 105 | 4 | 1 | 209 | 82 | 289 | 1.89e-67 | 209 |
| MS.gene60166.t1 | AT2G18800 | 47.534 | 223 | 102 | 4 | 1 | 209 | 75 | 296 | 2.08e-67 | 209 |
| MS.gene60166.t1 | AT1G32170 | 42.130 | 216 | 115 | 6 | 3 | 210 | 81 | 294 | 1.66e-50 | 167 |
| MS.gene60166.t1 | AT1G14720 | 42.056 | 214 | 116 | 6 | 3 | 210 | 80 | 291 | 1.07e-47 | 159 |
| MS.gene60166.t1 | AT3G48580 | 39.437 | 213 | 110 | 7 | 1 | 211 | 21 | 216 | 4.77e-45 | 149 |
| MS.gene60166.t1 | AT3G48580 | 39.437 | 213 | 110 | 7 | 1 | 211 | 80 | 275 | 1.45e-44 | 150 |
| MS.gene60166.t1 | AT2G36870 | 38.182 | 220 | 111 | 8 | 3 | 209 | 90 | 297 | 1.70e-44 | 150 |
| MS.gene60166.t1 | AT1G10550 | 36.036 | 222 | 127 | 6 | 2 | 209 | 89 | 309 | 2.59e-44 | 150 |
| MS.gene60166.t1 | AT3G44990 | 37.850 | 214 | 120 | 4 | 3 | 209 | 86 | 293 | 3.61e-44 | 149 |
| MS.gene60166.t1 | AT2G01850 | 38.967 | 213 | 124 | 5 | 3 | 210 | 80 | 291 | 1.09e-43 | 149 |
| MS.gene60166.t1 | AT4G18990 | 37.445 | 227 | 121 | 6 | 3 | 210 | 89 | 313 | 2.15e-43 | 149 |
| MS.gene60166.t1 | AT4G18990 | 37.500 | 200 | 106 | 5 | 28 | 210 | 42 | 239 | 7.79e-41 | 140 |
| MS.gene60166.t1 | AT3G48580 | 34.272 | 213 | 101 | 8 | 1 | 211 | 80 | 255 | 2.61e-29 | 110 |
| MS.gene60166.t1 | AT2G36870 | 48.315 | 89 | 39 | 2 | 3 | 84 | 90 | 178 | 1.86e-21 | 87.4 |
Find 48 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCCCATGGCTTGGATCTT+TGG | 0.186462 | 8.2:-24354291 | None:intergenic |
| TTCACACTTACTCAATTCTT+TGG | 0.200130 | 8.2:+24353653 | MS.gene60166:CDS |
| CATTATGGGAAGCTGATAAT+TGG | 0.203518 | 8.2:+24354322 | MS.gene60166:CDS |
| ACCCCACATGAGTGTCTTTC+AGG | 0.215363 | 8.2:+24354588 | MS.gene60166:CDS |
| ATTGTTCTTGTACACTCTTA+TGG | 0.245745 | 8.2:-24354247 | None:intergenic |
| GTGGATTAGAGAAAATTAAT+TGG | 0.259471 | 8.2:+24354355 | MS.gene60166:CDS |
| CTTTATTTACTTACACAATA+TGG | 0.267037 | 8.2:-24353684 | None:intergenic |
| GAGAACAAAGAGTTAATCTT+TGG | 0.282944 | 8.2:+24353611 | MS.gene60166:CDS |
| CTCCCATGGCTTGGATCTTT+GGG | 0.285110 | 8.2:-24354290 | None:intergenic |
| TTGTTCTTGTACACTCTTAT+GGG | 0.317433 | 8.2:-24354246 | None:intergenic |
| AGACACTCATGTGGGGTCAT+TGG | 0.379857 | 8.2:-24354582 | None:intergenic |
| GACACTCATGTGGGGTCATT+GGG | 0.402289 | 8.2:-24354581 | None:intergenic |
| GGGTTAGTGGCACATGTAGT+TGG | 0.414336 | 8.2:-24354441 | None:intergenic |
| ATTGAAGGATGTGCAATTCC+AGG | 0.423415 | 8.2:+24354417 | MS.gene60166:CDS |
| CCTATACCTTCTAGCTTCAA+TGG | 0.438940 | 8.2:-24354505 | None:intergenic |
| TACAAGAACAATGAAGCAAA+AGG | 0.440360 | 8.2:+24354258 | MS.gene60166:CDS |
| ATGTTTGTTTGAACTGTGTA+AGG | 0.451638 | 8.2:-24353565 | None:intergenic |
| ATTATGGGAAGCTGATAATT+GGG | 0.454763 | 8.2:+24354323 | MS.gene60166:CDS |
| CAAACAAACATTTATGCTCA+TGG | 0.456844 | 8.2:+24353577 | MS.gene60166:CDS |
| AACATTTATGCTCATGGAAA+GGG | 0.466489 | 8.2:+24353583 | MS.gene60166:CDS |
| GAGTTCTTGGGAAACCGTAC+TGG | 0.469916 | 8.2:+24353538 | MS.gene60166:CDS |
| GTGGCACATGTAGTTGGTCC+TGG | 0.486861 | 8.2:-24354435 | None:intergenic |
| ATGGCTTGGATCTTTGGGTA+TGG | 0.489548 | 8.2:-24354285 | None:intergenic |
| ACCTTTCTATGCTTATTACA+AGG | 0.510047 | 8.2:+24354386 | MS.gene60166:CDS |
| TTAGTGCCATTGAAGCTAGA+AGG | 0.511286 | 8.2:+24354499 | MS.gene60166:CDS |
| AAACATTTATGCTCATGGAA+AGG | 0.519040 | 8.2:+24353582 | MS.gene60166:CDS |
| ATTATTGTCAAGATAAATCA+AGG | 0.521408 | 8.2:+24354556 | MS.gene60166:CDS |
| TTGAAAATACTCCCATGGCT+TGG | 0.522806 | 8.2:-24354299 | None:intergenic |
| CTGTGTAAGGTTGTCCAGTA+CGG | 0.533081 | 8.2:-24353552 | None:intergenic |
| CCATTGAAGCTAGAAGGTAT+AGG | 0.547043 | 8.2:+24354505 | MS.gene60166:CDS |
| CTTGTACCTGGAGATTCTGC+TGG | 0.563207 | 8.2:+24353365 | MS.gene60166:CDS |
| TCCTTGTAATAAGCATAGAA+AGG | 0.566971 | 8.2:-24354387 | None:intergenic |
| ACAAGAACAATGAAGCAAAA+GGG | 0.575105 | 8.2:+24354259 | MS.gene60166:CDS |
| TGACAGTGTTCGTGATGAGT+TGG | 0.575375 | 8.2:+24353510 | MS.gene60166:CDS |
| GATAATTGGGCAACAAGAGG+TGG | 0.576591 | 8.2:+24354336 | MS.gene60166:CDS |
| TTGAAGCTAGAAGGTATAGG+TGG | 0.577676 | 8.2:+24354508 | MS.gene60166:CDS |
| TAAGTGTGAAAGTCAGCAGA+AGG | 0.588490 | 8.2:-24353640 | None:intergenic |
| TAATGTTGAAAATACTCCCA+TGG | 0.606737 | 8.2:-24354304 | None:intergenic |
| GACAATAATCATAGATCACA+TGG | 0.610597 | 8.2:-24354542 | None:intergenic |
| ACAGTACCAGCAGAATCTCC+AGG | 0.614116 | 8.2:-24353371 | None:intergenic |
| TGCCTGAAAGACACTCATGT+GGG | 0.615126 | 8.2:-24354590 | None:intergenic |
| ATGAAGATCAAACTTGTACC+TGG | 0.621043 | 8.2:+24353353 | MS.gene60166:CDS |
| TACCCAAAGATCCAAGCCAT+GGG | 0.632864 | 8.2:+24354288 | MS.gene60166:CDS |
| ATACCCAAAGATCCAAGCCA+TGG | 0.633713 | 8.2:+24354287 | MS.gene60166:CDS |
| GCTGATAATTGGGCAACAAG+AGG | 0.638048 | 8.2:+24354333 | MS.gene60166:CDS |
| TGAAGCTAGAAGGTATAGGT+GGG | 0.659236 | 8.2:+24354509 | MS.gene60166:CDS |
| GCCTGAAAGACACTCATGTG+GGG | 0.659861 | 8.2:-24354589 | None:intergenic |
| ATGCCTGAAAGACACTCATG+TGG | 0.662116 | 8.2:-24354591 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAATATTATATTCAGTTTT+TGG | - | chr8.2:24353463-24353482 | None:intergenic | 10.0% |
| !!! | ATAAAGAAATTATCTAATTT+TGG | + | chr8.2:24354055-24354074 | MS.gene60166:intron | 10.0% |
| !! | AGTTAAATACTTGAAAATTA+TGG | + | chr8.2:24353735-24353754 | MS.gene60166:intron | 15.0% |
| !!! | AAAAAAATACTTTGATTTTG+TGG | + | chr8.2:24354151-24354170 | MS.gene60166:intron | 15.0% |
| !!! | ATTTTGAAATTAAAGATAGT+CGG | - | chr8.2:24354037-24354056 | None:intergenic | 15.0% |
| !! | ATTATTGTCAAGATAAATCA+AGG | + | chr8.2:24354556-24354575 | MS.gene60166:CDS | 20.0% |
| !! | CTTAATAATGCAAAGAATTA+TGG | + | chr8.2:24353924-24353943 | MS.gene60166:intron | 20.0% |
| !!! | CTTTATTTACTTACACAATA+TGG | - | chr8.2:24353687-24353706 | None:intergenic | 20.0% |
| ! | ATGATGTTTCAAACAAAAAG+TGG | - | chr8.2:24353432-24353451 | None:intergenic | 25.0% |
| !!! | GTGGATTAGAGAAAATTAAT+TGG | + | chr8.2:24354355-24354374 | MS.gene60166:CDS | 25.0% |
| !!! | TACAAGGATTTTGATATTGA+AGG | + | chr8.2:24354402-24354421 | MS.gene60166:CDS | 25.0% |
| AAACATTTATGCTCATGGAA+AGG | + | chr8.2:24353582-24353601 | MS.gene60166:CDS | 30.0% | |
| AAAGAATTATGGCTAGACAT+GGG | + | chr8.2:24353935-24353954 | MS.gene60166:intron | 30.0% | |
| AACAACTTTGACAACTAACT+CGG | - | chr8.2:24353876-24353895 | None:intergenic | 30.0% | |
| AACATTTATGCTCATGGAAA+GGG | + | chr8.2:24353583-24353602 | MS.gene60166:CDS | 30.0% | |
| ACAAGAACAATGAAGCAAAA+GGG | + | chr8.2:24354259-24354278 | MS.gene60166:CDS | 30.0% | |
| ACCTTTCTATGCTTATTACA+AGG | + | chr8.2:24354386-24354405 | MS.gene60166:CDS | 30.0% | |
| ATGTTTGTTTGAACTGTGTA+AGG | - | chr8.2:24353568-24353587 | None:intergenic | 30.0% | |
| ATTATGGGAAGCTGATAATT+GGG | + | chr8.2:24354323-24354342 | MS.gene60166:CDS | 30.0% | |
| ATTGTTCTTGTACACTCTTA+TGG | - | chr8.2:24354250-24354269 | None:intergenic | 30.0% | |
| CAAACAAACATTTATGCTCA+TGG | + | chr8.2:24353577-24353596 | MS.gene60166:CDS | 30.0% | |
| GACAACATTGTGTCAAAAAA+AGG | - | chr8.2:24354121-24354140 | None:intergenic | 30.0% | |
| GACAATAATCATAGATCACA+TGG | - | chr8.2:24354545-24354564 | None:intergenic | 30.0% | |
| GAGAACAAAGAGTTAATCTT+TGG | + | chr8.2:24353611-24353630 | MS.gene60166:CDS | 30.0% | |
| GGCTAACTACATTTAGTATA+TGG | - | chr8.2:24353843-24353862 | None:intergenic | 30.0% | |
| TAATGTTGAAAATACTCCCA+TGG | - | chr8.2:24354307-24354326 | None:intergenic | 30.0% | |
| TACAAGAACAATGAAGCAAA+AGG | + | chr8.2:24354258-24354277 | MS.gene60166:CDS | 30.0% | |
| TCCTTGTAATAAGCATAGAA+AGG | - | chr8.2:24354390-24354409 | None:intergenic | 30.0% | |
| TTCACACTTACTCAATTCTT+TGG | + | chr8.2:24353653-24353672 | MS.gene60166:CDS | 30.0% | |
| TTGTTCTTGTACACTCTTAT+GGG | - | chr8.2:24354249-24354268 | None:intergenic | 30.0% | |
| !!! | GGGAGTATTTTCAACATTAT+GGG | + | chr8.2:24354308-24354327 | MS.gene60166:CDS | 30.0% |
| !!! | TGGGAGTATTTTCAACATTA+TGG | + | chr8.2:24354307-24354326 | MS.gene60166:CDS | 30.0% |
| AACTAACTCGGTTCAAATAG+TGG | - | chr8.2:24353864-24353883 | None:intergenic | 35.0% | |
| ATGAAGATCAAACTTGTACC+TGG | + | chr8.2:24353353-24353372 | MS.gene60166:CDS | 35.0% | |
| CAAAGAATTATGGCTAGACA+TGG | + | chr8.2:24353934-24353953 | MS.gene60166:intron | 35.0% | |
| CACTAACCCAAAAAATTGGT+GGG | + | chr8.2:24354455-24354474 | MS.gene60166:CDS | 35.0% | |
| CATTATGGGAAGCTGATAAT+TGG | + | chr8.2:24354322-24354341 | MS.gene60166:CDS | 35.0% | |
| CTAAGTGCTAACCACAAATT+TGG | - | chr8.2:24353770-24353789 | None:intergenic | 35.0% | |
| !!! | GAGTTGGATTTTGAGTTCTT+GGG | + | chr8.2:24353526-24353545 | MS.gene60166:CDS | 35.0% |
| !!! | TGAGTTGGATTTTGAGTTCT+TGG | + | chr8.2:24353525-24353544 | MS.gene60166:CDS | 35.0% |
| ACCCAAAAAATTGGTGGGAA+AGG | + | chr8.2:24354460-24354479 | MS.gene60166:CDS | 40.0% | |
| AGCACTTAGCAGAGTTACAT+TGG | + | chr8.2:24353781-24353800 | MS.gene60166:intron | 40.0% | |
| ATAGAAGCTGCATGTGTGTA+AGG | - | chr8.2:24354214-24354233 | None:intergenic | 40.0% | |
| ATTGAAGGATGTGCAATTCC+AGG | + | chr8.2:24354417-24354436 | MS.gene60166:CDS | 40.0% | |
| CCACTAACCCAAAAAATTGG+TGG | + | chr8.2:24354454-24354473 | MS.gene60166:CDS | 40.0% | |
| CCCAAAAAATTGGTGGGAAA+GGG | + | chr8.2:24354461-24354480 | MS.gene60166:CDS | 40.0% | |
| GCTAACCACAAATTTGGACA+TGG | - | chr8.2:24353764-24353783 | None:intergenic | 40.0% | |
| GGAAACCATGTCCAAATTTG+TGG | + | chr8.2:24353756-24353775 | MS.gene60166:intron | 40.0% | |
| TAAGTGTGAAAGTCAGCAGA+AGG | - | chr8.2:24353643-24353662 | None:intergenic | 40.0% | |
| TTGAAAATACTCCCATGGCT+TGG | - | chr8.2:24354302-24354321 | None:intergenic | 40.0% | |
| ! | CCATTGAAGCTAGAAGGTAT+AGG | + | chr8.2:24354505-24354524 | MS.gene60166:CDS | 40.0% |
| ! | GTGCCACTAACCCAAAAAAT+TGG | + | chr8.2:24354451-24354470 | MS.gene60166:CDS | 40.0% |
| ! | TGAAGCTAGAAGGTATAGGT+GGG | + | chr8.2:24354509-24354528 | MS.gene60166:CDS | 40.0% |
| ! | TTAGTGCCATTGAAGCTAGA+AGG | + | chr8.2:24354499-24354518 | MS.gene60166:CDS | 40.0% |
| ! | TTGAAGCTAGAAGGTATAGG+TGG | + | chr8.2:24354508-24354527 | MS.gene60166:CDS | 40.0% |
| !! | CCTATACCTTCTAGCTTCAA+TGG | - | chr8.2:24354508-24354527 | None:intergenic | 40.0% |
| !!! | ACCCTTTCCCACCAATTTTT+TGG | - | chr8.2:24354465-24354484 | None:intergenic | 40.0% |
| !!! | CCACCAATTTTTTGGGTTAG+TGG | - | chr8.2:24354457-24354476 | None:intergenic | 40.0% |
| !!! | CCCTTTCCCACCAATTTTTT+GGG | - | chr8.2:24354464-24354483 | None:intergenic | 40.0% |
| ATACCCAAAGATCCAAGCCA+TGG | + | chr8.2:24354287-24354306 | MS.gene60166:CDS | 45.0% | |
| ATGCCTGAAAGACACTCATG+TGG | - | chr8.2:24354594-24354613 | None:intergenic | 45.0% | |
| CTGTGTAAGGTTGTCCAGTA+CGG | - | chr8.2:24353555-24353574 | None:intergenic | 45.0% | |
| GATAATTGGGCAACAAGAGG+TGG | + | chr8.2:24354336-24354355 | MS.gene60166:CDS | 45.0% | |
| GCTGATAATTGGGCAACAAG+AGG | + | chr8.2:24354333-24354352 | MS.gene60166:CDS | 45.0% | |
| TACCCAAAGATCCAAGCCAT+GGG | + | chr8.2:24354288-24354307 | MS.gene60166:CDS | 45.0% | |
| TGACAGTGTTCGTGATGAGT+TGG | + | chr8.2:24353510-24353529 | MS.gene60166:CDS | 45.0% | |
| TGCCTGAAAGACACTCATGT+GGG | - | chr8.2:24354593-24354612 | None:intergenic | 45.0% | |
| ! | ATGGCTTGGATCTTTGGGTA+TGG | - | chr8.2:24354288-24354307 | None:intergenic | 45.0% |
| !!! | AATATAATATTTTTTTTTTG+TGG | + | chr8.2:24353470-24353489 | MS.gene60166:intron | 5.0% |
| ACAGTACCAGCAGAATCTCC+AGG | - | chr8.2:24353374-24353393 | None:intergenic | 50.0% | |
| ACCCCACATGAGTGTCTTTC+AGG | + | chr8.2:24354588-24354607 | MS.gene60166:CDS | 50.0% | |
| AGACACTCATGTGGGGTCAT+TGG | - | chr8.2:24354585-24354604 | None:intergenic | 50.0% | |
| CTTGTACCTGGAGATTCTGC+TGG | + | chr8.2:24353365-24353384 | MS.gene60166:CDS | 50.0% | |
| GACACTCATGTGGGGTCATT+GGG | - | chr8.2:24354584-24354603 | None:intergenic | 50.0% | |
| GAGTTCTTGGGAAACCGTAC+TGG | + | chr8.2:24353538-24353557 | MS.gene60166:CDS | 50.0% | |
| GCCTGAAAGACACTCATGTG+GGG | - | chr8.2:24354592-24354611 | None:intergenic | 50.0% | |
| ! | ACTCCCATGGCTTGGATCTT+TGG | - | chr8.2:24354294-24354313 | None:intergenic | 50.0% |
| ! | CTCCCATGGCTTGGATCTTT+GGG | - | chr8.2:24354293-24354312 | None:intergenic | 50.0% |
| ! | GGGTTAGTGGCACATGTAGT+TGG | - | chr8.2:24354444-24354463 | None:intergenic | 50.0% |
| GTGGCACATGTAGTTGGTCC+TGG | - | chr8.2:24354438-24354457 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 24353353 | 24354617 | 24353353 | ID=MS.gene60166 |
| chr8.2 | mRNA | 24353353 | 24354617 | 24353353 | ID=MS.gene60166.t1;Parent=MS.gene60166 |
| chr8.2 | exon | 24353353 | 24353406 | 24353353 | ID=MS.gene60166.t1.exon1;Parent=MS.gene60166.t1 |
| chr8.2 | CDS | 24353353 | 24353406 | 24353353 | ID=cds.MS.gene60166.t1;Parent=MS.gene60166.t1 |
| chr8.2 | exon | 24353496 | 24353692 | 24353496 | ID=MS.gene60166.t1.exon2;Parent=MS.gene60166.t1 |
| chr8.2 | CDS | 24353496 | 24353692 | 24353496 | ID=cds.MS.gene60166.t1;Parent=MS.gene60166.t1 |
| chr8.2 | exon | 24354227 | 24354617 | 24354227 | ID=MS.gene60166.t1.exon3;Parent=MS.gene60166.t1 |
| chr8.2 | CDS | 24354227 | 24354617 | 24354227 | ID=cds.MS.gene60166.t1;Parent=MS.gene60166.t1 |
| Gene Sequence |
| Protein sequence |
