AlfalfaGEDB | MS.gene60726

M. sativa cultivar XinJiangDaYe / MS.gene60726

KEGG: K09874
GO: GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0008150 GO:0015267 GO:0016020 GO:0022803 GO:0022857 GO:0051179 GO:0051234 GO:0055085 GO:0110165
PFAM: PF00230.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60726.t1	XP_003592016.1	99.3	300	2	0	1	300	1	300	5.40E-139	503.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60726.t1	Q9SV84	65.8	281	86	3	25	300	29	304	6.6e-85	315.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60726.t1	G7IAK3	99.3	300	2	0	1	300	1	300	3.9e-139	503.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049217	MS.gene60726	-0.800803	1.21E-48	-1.69E-46
MS.gene049549	MS.gene60726	0.812665	3.78E-51	-1.69E-46
MS.gene049611	MS.gene60726	0.807088	6.00E-50	-1.69E-46
MS.gene049633	MS.gene60726	-0.806915	6.52E-50	-1.69E-46
MS.gene049634	MS.gene60726	0.801919	7.17E-49	-1.69E-46
MS.gene050444	MS.gene60726	-0.800599	1.34E-48	-1.69E-46
MS.gene050675	MS.gene60726	-0.800766	1.24E-48	-1.69E-46
MS.gene051138	MS.gene60726	0.813155	2.95E-51	-1.69E-46
MS.gene051632	MS.gene60726	0.825781	3.89E-54	-1.69E-46
MS.gene052211	MS.gene60726	0.817886	2.61E-52	-1.69E-46
MS.gene05240	MS.gene60726	0.809081	2.26E-50	-1.69E-46
MS.gene05244	MS.gene60726	0.823294	1.50E-53	-1.69E-46
MS.gene053420	MS.gene60726	0.809434	1.90E-50	-1.69E-46
MS.gene053776	MS.gene60726	0.801494	8.77E-49	-1.69E-46
MS.gene054706	MS.gene60726	0.8054	1.36E-49	-1.69E-46
MS.gene054884	MS.gene60726	0.808213	3.46E-50	-1.69E-46
MS.gene055158	MS.gene60726	0.806521	7.90E-50	-1.69E-46
MS.gene055206	MS.gene60726	-0.801696	7.97E-49	-1.69E-46
MS.gene056590	MS.gene60726	0.829138	6.09E-55	-1.69E-46
MS.gene056591	MS.gene60726	0.825484	4.57E-54	-1.69E-46
MS.gene056688	MS.gene60726	0.807662	4.53E-50	-1.69E-46
MS.gene056748	MS.gene60726	0.820806	5.65E-53	-1.69E-46
MS.gene056856	MS.gene60726	0.845753	3.34E-59	-1.69E-46
MS.gene057261	MS.gene60726	0.801372	9.29E-49	-1.69E-46
MS.gene057717	MS.gene60726	0.81039	1.18E-50	-1.69E-46
MS.gene058067	MS.gene60726	0.803078	4.14E-49	-1.69E-46
MS.gene059677	MS.gene60726	-0.802128	6.50E-49	-1.69E-46
MS.gene059955	MS.gene60726	0.802965	4.37E-49	-1.69E-46
MS.gene060405	MS.gene60726	0.828531	8.54E-55	-1.69E-46
MS.gene060762	MS.gene60726	-0.808157	3.56E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene009458	MS.gene60726	PPI
MS.gene60726	MS.gene56256	PPI
MS.gene51627	MS.gene60726	PPI
MS.gene60726	MS.gene63514	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60726.t1	MTR_1g097840	99.333	300	2	0	1	300	1	300	0.0	602
MS.gene60726.t1	MTR_1g097840	99.153	236	2	0	1	236	1	236	4.24e-169	469
MS.gene60726.t1	MTR_4g006730	59.375	288	102	2	9	287	7	288	3.39e-113	329
MS.gene60726.t1	MTR_2g104940	56.908	304	111	6	13	300	11	310	4.53e-108	317
MS.gene60726.t1	MTR_4g006630	66.818	220	65	2	87	300	1	218	2.37e-98	288
MS.gene60726.t1	MTR_4g006730	66.818	220	65	2	87	300	1	218	7.21e-98	287
MS.gene60726.t1	MTR_4g006730	57.806	237	85	2	9	236	7	237	2.66e-84	254
MS.gene60726.t1	MTR_8g087720	49.356	233	107	3	71	300	35	259	4.38e-69	216
MS.gene60726.t1	MTR_8g087710	49.362	235	108	3	69	300	34	260	5.25e-69	216
MS.gene60726.t1	MTR_2g017610	47.600	250	112	4	54	300	26	259	2.24e-67	211
MS.gene60726.t1	MTR_2g017570	47.600	250	112	4	54	300	26	259	2.81e-67	211
MS.gene60726.t1	MTR_2g017660	50.673	223	108	2	62	283	28	249	1.07e-66	210
MS.gene60726.t1	MTR_2g017590	48.718	234	109	3	70	300	32	257	8.76e-66	207
MS.gene60726.t1	MTR_2g017620	48.696	230	113	2	74	300	38	265	8.14e-65	205
MS.gene60726.t1	MTR_2g017620	48.696	230	113	2	74	300	24	251	1.04e-64	204
MS.gene60726.t1	MTR_7g010650	44.749	219	121	0	72	290	35	253	2.14e-64	204
MS.gene60726.t1	MTR_4g084880	44.915	236	117	4	70	300	31	258	1.88e-61	196
MS.gene60726.t1	MTR_5g063930	39.706	272	137	5	28	294	6	255	8.52e-58	186
MS.gene60726.t1	MTR_2g017620	49.468	188	86	2	118	300	1	184	6.76e-55	177
MS.gene60726.t1	MTR_4g006650	64.539	141	41	1	75	215	5	136	5.67e-53	176
MS.gene60726.t1	MTR_5g079570	39.921	253	138	3	32	283	10	249	6.18e-52	172
MS.gene60726.t1	MTR_1g070385	43.304	224	127	0	69	292	57	280	1.93e-51	181
MS.gene60726.t1	MTR_3g008920	41.004	239	135	4	47	283	1	235	6.20e-49	164
MS.gene60726.t1	MTR_8g087720	50.888	169	78	2	71	236	35	201	3.89e-46	154
MS.gene60726.t1	MTR_8g087720	48.322	149	72	2	71	216	35	181	1.55e-34	124
MS.gene60726.t1	MTR_7g010650	41.379	145	85	0	72	216	35	179	1.54e-33	122
MS.gene60726.t1	MTR_2g101370	36.481	233	128	6	69	287	15	241	5.40e-31	117
MS.gene60726.t1	MTR_1g006490	35.841	226	126	7	74	287	19	237	3.49e-28	109
MS.gene60726.t1	MTR_8g013680	37.089	213	113	7	78	275	23	229	1.18e-27	108
MS.gene60726.t1	MTR_1g006590	35.981	214	118	7	74	275	19	225	2.05e-27	107
MS.gene60726.t1	MTR_1g115405	33.333	228	132	7	78	291	25	246	1.32e-22	94.7
MS.gene60726.t1	MTR_5g012810	33.333	234	134	5	78	298	23	247	3.53e-21	90.5
MS.gene60726.t1	MTR_2g094270	30.677	251	139	7	71	287	37	286	3.14e-19	85.9
MS.gene60726.t1	MTR_4g063090	32.870	216	123	8	79	279	26	234	8.75e-19	84.0
MS.gene60726.t1	MTR_7g023350	31.330	233	130	8	78	291	25	246	2.41e-18	82.8
MS.gene60726.t1	MTR_6g069040	28.571	224	149	4	78	293	25	245	7.84e-18	81.6
MS.gene60726.t1	MTR_7g103030	30.804	224	144	6	78	292	25	246	8.94e-17	78.6
MS.gene60726.t1	MTR_1g077540	30.357	224	145	5	78	293	25	245	1.19e-16	78.2
MS.gene60726.t1	MTR_3g118010	29.060	234	148	7	66	282	29	261	5.06e-16	77.0
MS.gene60726.t1	MTR_2g094270	30.476	210	124	5	71	259	37	245	1.72e-15	75.1
MS.gene60726.t1	MTR_2g060360	32.967	182	102	5	126	299	76	245	5.18e-15	73.6
MS.gene60726.t1	MTR_8g098375	29.646	226	139	8	78	285	56	279	2.19e-13	69.3
MS.gene60726.t1	MTR_4g415300	29.333	225	140	9	78	285	60	282	2.25e-13	69.3
MS.gene60726.t1	MTR_7g101190	27.092	251	157	8	71	299	36	282	2.43e-13	69.3
MS.gene60726.t1	MTR_3g070210	26.126	222	147	6	78	282	59	280	4.23e-13	68.6
MS.gene60726.t1	MTR_5g082070	26.667	225	147	6	78	285	59	282	9.10e-13	67.8
MS.gene60726.t1	MTR_5g010150	28.319	226	142	8	78	285	56	279	1.06e-12	67.4
MS.gene60726.t1	MTR_4g059390	25.591	254	157	7	66	287	31	284	2.75e-12	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60726.t1	AT4G10380	71.174	281	71	3	25	300	29	304	1.48e-143	406
MS.gene60726.t1	AT1G80760	56.550	313	101	4	9	300	7	305	6.07e-111	323
MS.gene60726.t1	AT5G37820	43.866	269	139	4	35	300	11	270	1.01e-68	215
MS.gene60726.t1	AT4G19030	49.167	240	108	3	69	300	50	283	1.35e-68	215
MS.gene60726.t1	AT5G37820	43.866	269	139	4	35	300	47	306	2.27e-68	216
MS.gene60726.t1	AT5G37810	42.435	271	140	4	35	300	11	270	1.46e-67	212
MS.gene60726.t1	AT4G18910	46.694	242	111	3	69	300	47	280	1.44e-65	207
MS.gene60726.t1	AT1G31885	50.673	223	102	2	69	283	38	260	5.10e-65	207
MS.gene60726.t1	AT1G31885	49.515	206	96	2	86	283	1	206	1.30e-57	186
MS.gene60726.t1	AT2G34390	43.777	233	117	4	69	291	43	271	2.07e-52	174
MS.gene60726.t1	AT2G34390	43.172	227	115	4	75	291	7	229	1.52e-50	167
MS.gene60726.t1	AT3G06100	44.762	210	113	3	78	285	50	258	3.11e-47	160
MS.gene60726.t1	AT3G06100	44.340	212	115	3	78	287	2	212	3.61e-47	158
MS.gene60726.t1	AT4G19030	49.143	175	81	2	69	235	50	224	3.10e-45	153
MS.gene60726.t1	AT4G18910	48.315	178	80	2	69	236	47	222	3.32e-44	150
MS.gene60726.t1	AT4G19030	46.795	156	75	2	69	216	50	205	1.51e-34	125
MS.gene60726.t1	AT2G34390	41.573	178	91	3	69	236	43	217	7.09e-32	118
MS.gene60726.t1	AT3G16240	37.500	208	110	7	78	271	23	224	1.05e-28	110
MS.gene60726.t1	AT2G29870	50.980	102	49	1	190	291	22	122	4.96e-26	100
MS.gene60726.t1	AT2G34390	40.625	160	82	3	69	218	43	199	2.41e-25	101
MS.gene60726.t1	AT5G47450	36.652	221	121	7	69	275	14	229	1.80e-24	99.8
MS.gene60726.t1	AT4G17340	35.849	212	117	6	78	275	23	229	5.05e-24	98.6
MS.gene60726.t1	AT2G25810	36.842	209	113	7	79	275	24	225	5.54e-24	98.2
MS.gene60726.t1	AT3G06100	39.855	138	82	1	149	285	40	177	2.68e-22	92.4
MS.gene60726.t1	AT3G26520	33.186	226	133	7	78	290	26	246	8.02e-22	92.4
MS.gene60726.t1	AT2G36830	34.348	230	129	8	78	292	25	247	2.06e-21	91.3
MS.gene60726.t1	AT4G01470	31.760	233	129	8	78	291	25	246	3.56e-19	85.1
MS.gene60726.t1	AT1G17810	29.258	229	148	5	78	293	28	255	2.00e-17	80.9
MS.gene60726.t1	AT1G31885	50.000	80	40	0	69	148	38	117	2.38e-17	77.4
MS.gene60726.t1	AT4G35100	29.600	250	156	7	66	299	30	275	8.05e-17	79.3
MS.gene60726.t1	AT4G35100	29.600	250	156	7	66	299	30	275	8.05e-17	79.3
MS.gene60726.t1	AT2G16850	29.796	245	152	7	71	299	33	273	1.01e-15	76.3
MS.gene60726.t1	AT1G73190	28.509	228	147	5	78	292	28	252	1.18e-15	75.9
MS.gene60726.t1	AT3G53420	27.376	263	164	8	59	297	24	283	8.00e-15	73.6
MS.gene60726.t1	AT3G53420	27.376	263	164	8	59	297	24	283	8.00e-15	73.6
MS.gene60726.t1	AT5G60660	26.383	235	147	7	72	282	37	269	3.42e-14	72.0
MS.gene60726.t1	AT2G37170	26.908	249	159	7	71	297	34	281	1.48e-13	70.1
MS.gene60726.t1	AT2G37170	26.908	249	159	7	71	297	77	324	2.41e-13	69.7
MS.gene60726.t1	AT3G54820	27.111	225	140	7	71	272	35	258	1.05e-12	67.4
MS.gene60726.t1	AT2G37180	26.104	249	161	7	71	297	34	281	2.67e-12	66.2
MS.gene60726.t1	AT2G45960	28.000	225	143	8	78	285	56	278	1.31e-11	64.3
MS.gene60726.t1	AT1G01620	27.488	211	137	6	78	272	56	266	1.59e-11	63.9
MS.gene60726.t1	AT3G61430	27.556	225	144	8	78	285	56	278	1.85e-11	63.9
MS.gene60726.t1	AT3G61430	27.556	225	144	8	78	285	56	278	1.85e-11	63.9
MS.gene60726.t1	AT3G47440	27.897	233	141	6	78	296	27	246	2.33e-11	63.2
MS.gene60726.t1	AT4G00430	27.232	224	146	7	78	285	57	279	2.74e-11	63.5
MS.gene60726.t1	AT4G23400	27.556	225	144	8	78	285	57	279	2.96e-11	63.2

Find 90 sgRNAs with CRISPR-Local

Find 226 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAAGCATCTCTGGATATATT+TGG	0.216287	1.2:-71245528	MS.gene60726:CDS
GGTGTCCCTGACTCCGATTC+TGG	0.274084	1.2:+71249890	None:intergenic
TTGTATTTGTTGTTCACTAT+TGG	0.275818	1.2:+71246584	None:intergenic
CAATGGAGTAGAAACTCTTA+TGG	0.319532	1.2:-71246564	MS.gene60726:CDS
ATCACTCACCATTGCCTTTG+CGG	0.329445	1.2:-71246462	MS.gene60726:CDS
CTTGGTGCTCTAGCTGGTTC+TGG	0.336743	1.2:-71245494	MS.gene60726:CDS
TTGCTCAACATTCTTATCTC+AGG	0.361959	1.2:-71245971	MS.gene60726:CDS
GAACATGTGCCCACGGGAAA+TGG	0.378646	1.2:+71246432	None:intergenic
ATGAATCCGGTGCGCACCTT+AGG	0.389490	1.2:-71245575	MS.gene60726:CDS
ATGTTCATTATTCTCTCAAT+TGG	0.394479	1.2:-71246512	MS.gene60726:CDS
TGCAATGCAGGTTGGTGAAT+TGG	0.400837	1.2:-71246020	MS.gene60726:intron
TGAACGTACCGCACGGGAAT+CGG	0.404673	1.2:+71246232	None:intergenic
TAAGGTGCGCACCGGATTCA+TGG	0.410875	1.2:+71245577	None:intergenic
CAGGAAATTACAAGCATCTC+TGG	0.418374	1.2:-71245538	MS.gene60726:CDS
TAATTTCCTGCTGCAACGGC+TGG	0.421426	1.2:+71245551	None:intergenic
CCGACGCTTGGTGCTCTAGC+TGG	0.422426	1.2:-71245500	MS.gene60726:CDS
AAAATGCCAGAATCGGAGTC+AGG	0.425265	1.2:-71249896	MS.gene60726:CDS
GTTTACCATCCTTTCCTCTC+TGG	0.425384	1.2:-71246350	MS.gene60726:CDS
AATGGCCCTCCGGGAGTATC+CGG	0.425506	1.2:+71249842	None:intergenic
TGCATCGCTAATTTCTCTGC+TGG	0.426344	1.2:-71249725	MS.gene60726:CDS
CTCTCAATTGGTCATATCTC+AGG	0.430898	1.2:-71246500	MS.gene60726:CDS
ATTCTTATAGATGAGAATAA+TGG	0.431504	1.2:+71249824	None:intergenic
CGGAGCTGAACCACCTCAAC+CGG	0.432157	1.2:-71245441	MS.gene60726:CDS
TGCTGCAACGGCTGGACCTA+AGG	0.434795	1.2:+71245559	None:intergenic
CAACGTGAGTCATTTGCAAT+GGG	0.443310	1.2:-71249788	MS.gene60726:CDS
CGTTATCACGAAGCTTGATA+AGG	0.443454	1.2:+71245462	None:intergenic
TTCATGGAACCACCGCTTGT+TGG	0.443747	1.2:+71245593	None:intergenic
CTTATCAAGCTTCGTGATAA+CGG	0.463299	1.2:-71245461	MS.gene60726:CDS
ATGGGAAATGCAGCTTGTGC+TGG	0.464614	1.2:-71246545	MS.gene60726:CDS
AATGGAGTAGAAACTCTTAT+GGG	0.469616	1.2:-71246563	MS.gene60726:CDS
GTTATCACGAAGCTTGATAA+GGG	0.481400	1.2:+71245463	None:intergenic
CTGAACCACCTCAACCGGTT+AGG	0.481691	1.2:-71245436	MS.gene60726:CDS
TAGCGACGGAAGCTCCTAAC+CGG	0.489899	1.2:+71245422	None:intergenic
CTTGTAATTTCCTGCTGCAA+CGG	0.491803	1.2:+71245547	None:intergenic
CGGCGTTTCGCCATTTCCCG+TGG	0.493217	1.2:-71246442	MS.gene60726:CDS
ACTTGTGCTGCAATGTAGGC+TGG	0.494118	1.2:+71246410	None:intergenic
GAGGTGCAACATGTGTATGC+CGG	0.494142	1.2:-71249765	MS.gene60726:CDS
TATTTGGTGGCACCGACGCT+TGG	0.495981	1.2:-71245512	MS.gene60726:CDS
TTAGGTCCAGCCGTTGCAGC+AGG	0.497485	1.2:-71245557	MS.gene60726:CDS
CGGAAGCTCCTAACCGGTTG+AGG	0.497802	1.2:+71245428	None:intergenic
AAGCGGTGGTTCCATGAATC+CGG	0.497866	1.2:-71245588	MS.gene60726:CDS
CAGATTGGAGCAGAGTTTGT+GGG	0.502703	1.2:-71246641	MS.gene60726:CDS
TGCTCAACATTCTTATCTCA+GGG	0.505305	1.2:-71245970	MS.gene60726:intron
GCAGATTGGAGCAGAGTTTG+TGG	0.507450	1.2:-71246642	MS.gene60726:intron
AGCGTCGGTGCCGGCGACAC+CGG	0.511138	1.2:-71249861	MS.gene60726:CDS
AGGTGCAACATGTGTATGCC+GGG	0.512535	1.2:-71249764	MS.gene60726:CDS
GAATTGGCTGGTATTGCTGT+TGG	0.524769	1.2:-71246004	MS.gene60726:CDS
GATGAGAATAATGGCCCTCC+GGG	0.524874	1.2:+71249833	None:intergenic
AGATGAGAATAATGGCCCTC+CGG	0.525358	1.2:+71249832	None:intergenic
GCGAAACGCCGCAAAGGCAA+TGG	0.530668	1.2:+71246454	None:intergenic
AAGTGTCTCTCTTTCTCAGA+AGG	0.536928	1.2:-71249690	MS.gene60726:intron
CCGGGAGTATCCGGTGTCGC+CGG	0.538656	1.2:+71249851	None:intergenic
AGAGACTTGTGCTGCAATGT+AGG	0.541162	1.2:+71246406	None:intergenic
ACCGACGGCAGCGTCGGTGC+CGG	0.542854	1.2:-71249870	MS.gene60726:CDS
TCAACGTGAGTCATTTGCAA+TGG	0.548537	1.2:-71249789	MS.gene60726:CDS
TTCTGAGAAAGAGAGACACT+TGG	0.567115	1.2:+71249692	None:intergenic
GAAATGGCGAAACGCCGCAA+AGG	0.567657	1.2:+71246448	None:intergenic
GGCGTTTCGCCATTTCCCGT+GGG	0.569511	1.2:-71246441	MS.gene60726:CDS
GCCGGCACCGACGCTGCCGT+CGG	0.571291	1.2:+71249869	None:intergenic
CTAATATATGCAGCAACAGC+AGG	0.571804	1.2:-71246608	MS.gene60726:CDS
AAATGCCAGAATCGGAGTCA+GGG	0.577507	1.2:-71249895	MS.gene60726:CDS
TACCATCCTTTCCTCTCTGG+CGG	0.579626	1.2:-71246347	MS.gene60726:CDS
CCGGCGACACCGGATACTCC+CGG	0.580871	1.2:-71249851	MS.gene60726:CDS
TGCGCTTGTTATGCGCTCAA+AGG	0.581568	1.2:-71246374	MS.gene60726:CDS
GCAAAGGCAATGGTGAGTGA+TGG	0.586293	1.2:+71246464	None:intergenic
GCATCTCTGGATATATTTGG+TGG	0.589329	1.2:-71245525	MS.gene60726:CDS
ATGCAGGTTGGTGAATTGGC+TGG	0.597369	1.2:-71246016	MS.gene60726:intron
ATGAATTGAACGTACCGCAC+GGG	0.604011	1.2:+71246226	None:intergenic
GCTGGACCTAAGGTGCGCAC+CGG	0.614709	1.2:+71245569	None:intergenic
TAGGCTGGAACATGTGCCCA+CGG	0.616186	1.2:+71246425	None:intergenic
GTCGCAGCCAGCTCTAGCGA+CGG	0.626232	1.2:+71245408	None:intergenic
ATAGTGAACAACAAATACAA+TGG	0.626790	1.2:-71246581	MS.gene60726:CDS
CCAGCTAGAGCACCAAGCGT+CGG	0.629619	1.2:+71245500	None:intergenic
AGGCTGGAACATGTGCCCAC+GGG	0.634760	1.2:+71246426	None:intergenic
ACTGTGCCTACTGTTAGCGT+TGG	0.635520	1.2:-71246320	MS.gene60726:CDS
CACCGCCAGAGAGGAAAGGA+TGG	0.636220	1.2:+71246345	None:intergenic
AGGGACACCGACGGCAGCGT+CGG	0.638264	1.2:-71249876	MS.gene60726:CDS
GTGACACCGCCAGAGAGGAA+AGG	0.639081	1.2:+71246341	None:intergenic
GCTTGTCCAACGCTAACAGT+AGG	0.640127	1.2:+71246314	None:intergenic
GTGAGTCATTTGCAATGGGG+AGG	0.644380	1.2:-71249784	MS.gene60726:CDS
AAGCTCCTAACCGGTTGAGG+TGG	0.655759	1.2:+71245431	None:intergenic
AATGCTAGGCCAACAAGCGG+TGG	0.661522	1.2:-71245602	MS.gene60726:intron
ATCGGAGTCAGGGACACCGA+CGG	0.669278	1.2:-71249885	MS.gene60726:CDS
GTAAATGCTAGGCCAACAAG+CGG	0.699062	1.2:-71245605	MS.gene60726:intron
CGACACCGGATACTCCCGGA+GGG	0.703914	1.2:-71249847	MS.gene60726:CDS
GCACAGTGACACCGCCAGAG+AGG	0.707983	1.2:+71246336	None:intergenic
AACGTGAGTCATTTGCAATG+GGG	0.719998	1.2:-71249787	MS.gene60726:CDS
GCGACACCGGATACTCCCGG+AGG	0.726349	1.2:-71249848	MS.gene60726:CDS
TGTTGCTACCGATTCCCGTG+CGG	0.745096	1.2:-71246240	MS.gene60726:intron
TGCAACATGTGTATGCCGGG+TGG	0.746549	1.2:-71249761	MS.gene60726:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTAATTTAATTAACAATT+GGG	-	chr1.2:71245932-71245951	MS.gene60726:intron	10.0%
!!	TAGTTAATTTAATTAACAAT+TGG	-	chr1.2:71245931-71245950	MS.gene60726:intron	10.0%
!!	TTATATTGAAATGAAATATA+TGG	-	chr1.2:71246541-71246560	MS.gene60726:CDS	10.0%
!!!	ATAAAAAAAAATTGTTTTCA+AGG	+	chr1.2:71246316-71246335	None:intergenic	10.0%
!!!	ATTTCAATATATAATTTTGT+CGG	-	chr1.2:71246593-71246612	MS.gene60726:CDS	10.0%
!!	AAAACTATTCAATATACTTT+CGG	+	chr1.2:71246880-71246899	None:intergenic	15.0%
!!	ATTCAGACAAAATTTATATT+CGG	+	chr1.2:71249545-71249564	None:intergenic	15.0%
!!	TACATATACTATATTATACA+CGG	+	chr1.2:71246351-71246370	None:intergenic	15.0%
!!!	AAAAAAAAGTTGATTAAATG+AGG	-	chr1.2:71247529-71247548	MS.gene60726:intron	15.0%
!!!	AAAAAAAGTTGATTAAATGA+GGG	-	chr1.2:71247530-71247549	MS.gene60726:intron	15.0%
!!!	AAATAGATAGCATTTTTTTT+AGG	-	chr1.2:71245838-71245857	MS.gene60726:intron	15.0%
!!!	AATAGATAGCATTTTTTTTA+GGG	-	chr1.2:71245839-71245858	MS.gene60726:intron	15.0%
!!!	ATAAGTTGTTTGTTTTTTTT+TGG	+	chr1.2:71248230-71248249	None:intergenic	15.0%
!!!	GAAATTACATAGATTTTTTT+TGG	-	chr1.2:71248036-71248055	MS.gene60726:intron	15.0%
!!!	GAATGTATATTTTTTTTTGA+AGG	+	chr1.2:71246228-71246247	None:intergenic	15.0%
!!!	TAAGCAAAAATTGATTTTTT+AGG	-	chr1.2:71247045-71247064	MS.gene60726:intron	15.0%
!!!	TACGTTTCTTTTTTAAAATA+CGG	+	chr1.2:71247422-71247441	None:intergenic	15.0%
!!!	TTGGATTTATTTTTTTTGAA+AGG	-	chr1.2:71247670-71247689	MS.gene60726:intron	15.0%
!!!	TTTGTTTGCTTTTTTTTTTT+AGG	+	chr1.2:71246270-71246289	None:intergenic	15.0%
!!	AAACTCAAAGTTTAAGTATT+CGG	+	chr1.2:71247581-71247600	None:intergenic	20.0%
!!	ACAAAATTAAATCAACTACA+TGG	+	chr1.2:71248313-71248332	None:intergenic	20.0%
!!	ATTACAAATACAAAAACAAG+CGG	+	chr1.2:71245963-71245982	None:intergenic	20.0%
!!	ATTCTTATAGATGAGAATAA+TGG	+	chr1.2:71245493-71245512	None:intergenic	20.0%
!!	ATTGTTTAAACAACTAAAAG+TGG	+	chr1.2:71247930-71247949	None:intergenic	20.0%
!!	TTTCATTGATAAACAAAAGA+TGG	-	chr1.2:71246661-71246680	MS.gene60726:intron	20.0%
!!!	AAAAAAGTTGATTAAATGAG+GGG	-	chr1.2:71247531-71247550	MS.gene60726:intron	20.0%
!!!	AAATTTCAAGTTCTCTAAAT+TGG	-	chr1.2:71249188-71249207	MS.gene60726:intron	20.0%
!!!	AACATGTTTGATATTTTCTT+AGG	+	chr1.2:71247491-71247510	None:intergenic	20.0%
!!!	AATGTATTTTTGAATACACA+TGG	-	chr1.2:71248447-71248466	MS.gene60726:intron	20.0%
!!!	AATTTCAAGTTCTCTAAATT+GGG	-	chr1.2:71249189-71249208	MS.gene60726:intron	20.0%
!!!	ACATGTTTGATATTTTCTTA+GGG	+	chr1.2:71247490-71247509	None:intergenic	20.0%
!!!	AGGAAATAAATTTTATTTGC+TGG	+	chr1.2:71246250-71246269	None:intergenic	20.0%
!!!	AGTTAAGTTGTTTTAATTCA+TGG	-	chr1.2:71249255-71249274	MS.gene60726:intron	20.0%
!!!	ATTCAATGATAATGTTTCAA+AGG	-	chr1.2:71249455-71249474	MS.gene60726:intron	20.0%
!!!	ATTTAATTTTGTAAGCTTTG+GGG	-	chr1.2:71248321-71248340	MS.gene60726:intron	20.0%
!!!	ATTTTTTTTGAAAGGAACTT+GGG	-	chr1.2:71247678-71247697	MS.gene60726:intron	20.0%
!!!	GATTTAATTTTGTAAGCTTT+GGG	-	chr1.2:71248320-71248339	MS.gene60726:intron	20.0%
!!!	TAATGTATTTTGACATAGAT+GGG	-	chr1.2:71246621-71246640	MS.gene60726:CDS	20.0%
!!!	TACTTTTCCTTTTTCATTAA+GGG	+	chr1.2:71248617-71248636	None:intergenic	20.0%
!!!	TATTTTTTTTGAAAGGAACT+TGG	-	chr1.2:71247677-71247696	MS.gene60726:intron	20.0%
!!!	TCTATTTGATATTTTAGTCA+AGG	+	chr1.2:71245825-71245844	None:intergenic	20.0%
!!!	TGATGTTATTGATTTGTATT+TGG	-	chr1.2:71249389-71249408	MS.gene60726:intron	20.0%
!!!	TGATTTAATTTTGTAAGCTT+TGG	-	chr1.2:71248319-71248338	MS.gene60726:intron	20.0%
!!!	TTACTTTTCCTTTTTCATTA+AGG	+	chr1.2:71248618-71248637	None:intergenic	20.0%
!!!	TTTTTCCAAAAATCTACATT+TGG	-	chr1.2:71248487-71248506	MS.gene60726:intron	20.0%
!	ATAGTGAACAACAAATACAA+TGG	-	chr1.2:71248733-71248752	MS.gene60726:intron	25.0%
!	ATCATGAAAAACTATTCCTA+AGG	-	chr1.2:71249483-71249502	MS.gene60726:intron	25.0%
!	ATGTTCATTATTCTCTCAAT+TGG	-	chr1.2:71248802-71248821	MS.gene60726:intron	25.0%
!	CTATGTCAAAATACATTAGA+AGG	+	chr1.2:71246619-71246638	None:intergenic	25.0%
!	TAACATCAATTCATGAGAAA+AGG	+	chr1.2:71249377-71249396	None:intergenic	25.0%
!	TGTCAAATGTTATATTCAGT+TGG	-	chr1.2:71249431-71249450	MS.gene60726:intron	25.0%
!!	AAACAGTCTCAAAGTATTTA+TGG	+	chr1.2:71248175-71248194	None:intergenic	25.0%
!!	AACCAAATGGAATTAATTTG+TGG	+	chr1.2:71245730-71245749	None:intergenic	25.0%
!!	AATTTTGTCTGAATAATGAC+AGG	-	chr1.2:71249551-71249570	MS.gene60726:intron	25.0%
!!	ATATTTTCTTAGGGTGTATT+TGG	+	chr1.2:71247481-71247500	None:intergenic	25.0%
!!	ATCTCTCTCTTTATGATTTT+TGG	-	chr1.2:71245651-71245670	MS.gene60726:intron	25.0%
!!	ATGTTCCAAATGTAGATTTT+TGG	+	chr1.2:71248495-71248514	None:intergenic	25.0%
!!	CTTTTGCTTATAATAGTGAT+CGG	-	chr1.2:71247151-71247170	MS.gene60726:intron	25.0%
!!	TTTTTGCTTATAATAGTGAC+CGG	+	chr1.2:71247035-71247054	None:intergenic	25.0%
!!!	ACCAAATGGAATTAATTTGT+GGG	+	chr1.2:71245729-71245748	None:intergenic	25.0%
!!!	ACTTTTCCTTTTTCATTAAG+GGG	+	chr1.2:71248616-71248635	None:intergenic	25.0%
!!!	AGGAGGTATATATTTTACTA+AGG	-	chr1.2:71247227-71247246	MS.gene60726:intron	25.0%
!!!	CTAATGTATTTTGACATAGA+TGG	-	chr1.2:71246620-71246639	MS.gene60726:CDS	25.0%
!!!	GGAGGTATATATTTTACTAA+GGG	-	chr1.2:71247228-71247247	MS.gene60726:intron	25.0%
!!!	TATTTTTGTATATGCAATGC+AGG	-	chr1.2:71249282-71249301	MS.gene60726:intron	25.0%
!!!	TTGTATTTGTTGTTCACTAT+TGG	+	chr1.2:71248733-71248752	None:intergenic	25.0%
!!!	TTTTTTAAGGGAATGAACTT+GGG	-	chr1.2:71247709-71247728	MS.gene60726:intron	25.0%
!!!	TTTTTTTAAGGGAATGAACT+TGG	-	chr1.2:71247708-71247727	MS.gene60726:intron	25.0%
!!!	TTTTTTTTGAAGGAATTTGC+TGG	+	chr1.2:71246218-71246237	None:intergenic	25.0%
	AACAAATGCAACTAGTAACA+TGG	+	chr1.2:71246816-71246835	None:intergenic	30.0%
	AATGGAGTAGAAACTCTTAT+GGG	-	chr1.2:71248751-71248770	MS.gene60726:intron	30.0%
	ACAAATGCAACTAGTAACAT+GGG	+	chr1.2:71246815-71246834	None:intergenic	30.0%
	ACTAGTAACATGGGTTTATT+AGG	+	chr1.2:71246806-71246825	None:intergenic	30.0%
	ATATGACAGCCAAAATTTCA+AGG	-	chr1.2:71247181-71247200	MS.gene60726:intron	30.0%
	ATTTGGATTGAGAGTTTATG+AGG	+	chr1.2:71247464-71247483	None:intergenic	30.0%
	CTAGTAACATGGGTTTATTA+GGG	+	chr1.2:71246805-71246824	None:intergenic	30.0%
	GCATAAATAGTATTGTATGC+AGG	-	chr1.2:71246048-71246067	MS.gene60726:intron	30.0%
	GGATTAGAATAATGTGACAA+TGG	+	chr1.2:71248594-71248613	None:intergenic	30.0%
	GTCAGATTTAACTGTTTCAT+GGG	-	chr1.2:71245763-71245782	MS.gene60726:intron	30.0%
	GTCATGTCATTAAAAACCTT+AGG	+	chr1.2:71249502-71249521	None:intergenic	30.0%
	TAAATAGTATTGTATGCAGG+TGG	-	chr1.2:71246051-71246070	MS.gene60726:intron	30.0%
	TAAGATTGTGAGTAATGTTG+TGG	-	chr1.2:71249684-71249703	MS.gene60726:intron	30.0%
	TAGGTTAACAAGTGTCATAA+GGG	-	chr1.2:71247807-71247826	MS.gene60726:intron	30.0%
	TTAGAGAACTTGAAATTTCG+CGG	+	chr1.2:71249186-71249205	None:intergenic	30.0%
	TTAGGTTAACAAGTGTCATA+AGG	-	chr1.2:71247806-71247825	MS.gene60726:intron	30.0%
	TTTGGATTGAGAGTTTATGA+GGG	+	chr1.2:71247463-71247482	None:intergenic	30.0%
!	ATGTTTTCTCAATATCAAGG+AGG	-	chr1.2:71247210-71247229	MS.gene60726:intron	30.0%
!	GACATGTTTTCTCAATATCA+AGG	-	chr1.2:71247207-71247226	MS.gene60726:intron	30.0%
!!	AAATGGAATTAATTTGTGGG+CGG	+	chr1.2:71245726-71245745	None:intergenic	30.0%
!!	CTTTTCCTTTTTCATTAAGG+GGG	+	chr1.2:71248615-71248634	None:intergenic	30.0%
!!	TATATTTTACTAAGGGTGTG+TGG	-	chr1.2:71247235-71247254	MS.gene60726:intron	30.0%
!!!	GATTTTTGGTCAACGAATTT+TGG	-	chr1.2:71245665-71245684	MS.gene60726:intron	30.0%
!!!	TGGGTTCGACTTTTTTTTAA+GGG	-	chr1.2:71247697-71247716	MS.gene60726:intron	30.0%
!!!	TTGGGTTCGACTTTTTTTTA+AGG	-	chr1.2:71247696-71247715	MS.gene60726:intron	30.0%
!!!	TTTTGACATAGATGGGTTAT+TGG	-	chr1.2:71246628-71246647	MS.gene60726:CDS	30.0%
	AACATTCTTATCTCAGGGTA+TGG	-	chr1.2:71249349-71249368	MS.gene60726:intron	35.0%
	AGCATTGCTTGAGTTATTAG+TGG	-	chr1.2:71248398-71248417	MS.gene60726:intron	35.0%
	AGTTTATGAGGGAAAAGGAA+GGG	+	chr1.2:71247452-71247471	None:intergenic	35.0%
	ATATTCGGAGACTAACTTGA+TGG	+	chr1.2:71249530-71249549	None:intergenic	35.0%
	ATCGTGTCAATCTGAAAACT+TGG	-	chr1.2:71247645-71247664	MS.gene60726:intron	35.0%
	ATGTTGTGGTTGTAAATGCT+AGG	-	chr1.2:71249698-71249717	MS.gene60726:CDS	35.0%
	CAAGCATCTCTGGATATATT+TGG	-	chr1.2:71249786-71249805	MS.gene60726:CDS	35.0%
	CAATGGAGTAGAAACTCTTA+TGG	-	chr1.2:71248750-71248769	MS.gene60726:intron	35.0%
	CTAATCCCCCTTAATGAAAA+AGG	-	chr1.2:71248607-71248626	MS.gene60726:intron	35.0%
	CTACATGGTTAACCTTTGTT+TGG	+	chr1.2:71248298-71248317	None:intergenic	35.0%
	CTTATCAAGCTTCGTGATAA+CGG	-	chr1.2:71249853-71249872	MS.gene60726:CDS	35.0%
	GAAACAGTGCAAAAACATGT+AGG	-	chr1.2:71246710-71246729	MS.gene60726:intron	35.0%
	GCCCACAAATTAATTCCATT+TGG	-	chr1.2:71245725-71245744	MS.gene60726:intron	35.0%
	GGTCAGATTTAACTGTTTCA+TGG	-	chr1.2:71245762-71245781	MS.gene60726:intron	35.0%
	GTAGAATGAAAGAGCTACTT+TGG	+	chr1.2:71248529-71248548	None:intergenic	35.0%
	GTTATCACGAAGCTTGATAA+GGG	+	chr1.2:71249854-71249873	None:intergenic	35.0%
	TCAATCTGAAAACTTGGGTT+TGG	-	chr1.2:71247651-71247670	MS.gene60726:intron	35.0%
	TCGTGTCAATCTGAAAACTT+GGG	-	chr1.2:71247646-71247665	MS.gene60726:intron	35.0%
	TGCTCAACATTCTTATCTCA+GGG	-	chr1.2:71249344-71249363	MS.gene60726:intron	35.0%
	TGTAGGATATTAAGCTACAC+TGG	-	chr1.2:71246727-71246746	MS.gene60726:intron	35.0%
	TGTTAAAATGTGTCGCAGAT+TGG	-	chr1.2:71248658-71248677	MS.gene60726:intron	35.0%
	TTCAAAAGCTCTCCAAACAA+AGG	-	chr1.2:71248283-71248302	MS.gene60726:intron	35.0%
	TTGAGAGTTTATGAGGGAAA+AGG	+	chr1.2:71247457-71247476	None:intergenic	35.0%
	TTGATGAGATGTGCATGATT+GGG	-	chr1.2:71246009-71246028	MS.gene60726:CDS	35.0%
	TTGCTCAACATTCTTATCTC+AGG	-	chr1.2:71249343-71249362	MS.gene60726:intron	35.0%
	TTGCTTATAATAGTGATCGG+AGG	-	chr1.2:71247154-71247173	MS.gene60726:intron	35.0%
	TTTGATGAGATGTGCATGAT+TGG	-	chr1.2:71246008-71246027	MS.gene60726:CDS	35.0%
	TTTGTATATGCAATGCAGGT+TGG	-	chr1.2:71249286-71249305	MS.gene60726:intron	35.0%
!	AAGTTGATTAAATGAGGGGT+AGG	-	chr1.2:71247535-71247554	MS.gene60726:intron	35.0%
!	GAATGATGATGTGTGTACAT+AGG	-	chr1.2:71248347-71248366	MS.gene60726:intron	35.0%
!	TTAAGCTACACTGGTTATCT+TGG	-	chr1.2:71246736-71246755	MS.gene60726:intron	35.0%
!!	AGAGCTACTTTGGAGTTTAT+TGG	+	chr1.2:71248519-71248538	None:intergenic	35.0%
	AACGTGAGTCATTTGCAATG+GGG	-	chr1.2:71245527-71245546	MS.gene60726:CDS	40.0%
	AAGTGTCTCTCTTTCTCAGA+AGG	-	chr1.2:71245624-71245643	MS.gene60726:intron	40.0%
	AATGAATTGAACGTACCGCA+CGG	+	chr1.2:71249092-71249111	None:intergenic	40.0%
	ATGGAGAGTTTAAGAGAGGA+TGG	-	chr1.2:71247258-71247277	MS.gene60726:intron	40.0%
	CAACGTGAGTCATTTGCAAT+GGG	-	chr1.2:71245526-71245545	MS.gene60726:CDS	40.0%
	CAGGAAATTACAAGCATCTC+TGG	-	chr1.2:71249776-71249795	MS.gene60726:CDS	40.0%
	CATGCAAGCTAGAAACCAAA+TGG	+	chr1.2:71245743-71245762	None:intergenic	40.0%
	CGTTATCACGAAGCTTGATA+AGG	+	chr1.2:71249855-71249874	None:intergenic	40.0%
	CTAATATATGCAGCAACAGC+AGG	-	chr1.2:71248706-71248725	MS.gene60726:intron	40.0%
	CTCTCAATTGGTCATATCTC+AGG	-	chr1.2:71248814-71248833	MS.gene60726:intron	40.0%
	CTTGTAATTTCCTGCTGCAA+CGG	+	chr1.2:71249770-71249789	None:intergenic	40.0%
	GACAGCCAAAATTTCAAGGA+CGG	-	chr1.2:71247185-71247204	MS.gene60726:intron	40.0%
	GAGTTTATGAGGGAAAAGGA+AGG	+	chr1.2:71247453-71247472	None:intergenic	40.0%
	GCATCTCTGGATATATTTGG+TGG	-	chr1.2:71249789-71249808	MS.gene60726:CDS	40.0%
	GTCTGAATAATGACAGGATC+AGG	-	chr1.2:71249557-71249576	MS.gene60726:intron	40.0%
	GTTTATGAGGGAAAAGGAAG+GGG	+	chr1.2:71247451-71247470	None:intergenic	40.0%
	TACTTAACTCGTACTTCCTC+CGG	-	chr1.2:71247013-71247032	MS.gene60726:intron	40.0%
	TCAACGTGAGTCATTTGCAA+TGG	-	chr1.2:71245525-71245544	MS.gene60726:CDS	40.0%
	TGGAGAGTTTAAGAGAGGAT+GGG	-	chr1.2:71247259-71247278	MS.gene60726:intron	40.0%
	TTGCTTATAATAGTGACCGG+AGG	+	chr1.2:71247032-71247051	None:intergenic	40.0%
!	CATGTCCGTCCTTGAAATTT+TGG	+	chr1.2:71247193-71247212	None:intergenic	40.0%
!	TTCTGAGAAAGAGAGACACT+TGG	+	chr1.2:71245625-71245644	None:intergenic	40.0%
!!	CAACTATTGCTTTTACCACC+CGG	+	chr1.2:71245571-71245590	None:intergenic	40.0%
!!	CATTTGGTTTCTAGCTTGCA+TGG	-	chr1.2:71245741-71245760	MS.gene60726:intron	40.0%
!!	TGTCATAAGGGCATTGTTTG+AGG	-	chr1.2:71247819-71247838	MS.gene60726:intron	40.0%
!!	TTTCGCGGCAACTTTTTAAG+TGG	+	chr1.2:71249171-71249190	None:intergenic	40.0%
!!	TTTTACTAAGGGTGTGTGGA+TGG	-	chr1.2:71247239-71247258	MS.gene60726:intron	40.0%
	AGAGACTTGTGCTGCAATGT+AGG	+	chr1.2:71248911-71248930	None:intergenic	45.0%
	AGAGTTTAAGAGAGGATGGG+AGG	-	chr1.2:71247262-71247281	MS.gene60726:intron	45.0%
	AGATGAGAATAATGGCCCTC+CGG	+	chr1.2:71245485-71245504	None:intergenic	45.0%
	AGTTTAAGAGAGGATGGGAG+GGG	-	chr1.2:71247264-71247283	MS.gene60726:intron	45.0%
	ATCACTCACCATTGCCTTTG+CGG	-	chr1.2:71248852-71248871	MS.gene60726:intron	45.0%
	ATGAATTGAACGTACCGCAC+GGG	+	chr1.2:71249091-71249110	None:intergenic	45.0%
	CAGATTGGAGCAGAGTTTGT+GGG	-	chr1.2:71248673-71248692	MS.gene60726:intron	45.0%
	GAATTGGCTGGTATTGCTGT+TGG	-	chr1.2:71249310-71249329	MS.gene60726:intron	45.0%
	GAGTTTAAGAGAGGATGGGA+GGG	-	chr1.2:71247263-71247282	MS.gene60726:intron	45.0%
	GTAAATGCTAGGCCAACAAG+CGG	-	chr1.2:71249709-71249728	MS.gene60726:CDS	45.0%
	GTGGATGGAGAGTTTAAGAG+AGG	-	chr1.2:71247254-71247273	MS.gene60726:intron	45.0%
	GTTTACCATCCTTTCCTCTC+TGG	-	chr1.2:71248964-71248983	MS.gene60726:intron	45.0%
	TGCATCGCTAATTTCTCTGC+TGG	-	chr1.2:71245589-71245608	MS.gene60726:CDS	45.0%
!!	TGCAATGCAGGTTGGTGAAT+TGG	-	chr1.2:71249294-71249313	MS.gene60726:intron	45.0%
!!	TGGAATTAATTTGTGGGCGG+TGG	+	chr1.2:71245723-71245742	None:intergenic	45.0%
!!!	AATTTTTAAAAAAAAAAGTT+AGG	-	chr1.2:71247788-71247807	MS.gene60726:intron	5.0%
!!!	TTTATTTTACATTTATTTTT+AGG	+	chr1.2:71247978-71247997	None:intergenic	5.0%
	AAGCGGTGGTTCCATGAATC+CGG	-	chr1.2:71249726-71249745	MS.gene60726:CDS	50.0%
	ACTGTGCCTACTGTTAGCGT+TGG	-	chr1.2:71248994-71249013	MS.gene60726:intron	50.0%
	ACTTGTGCTGCAATGTAGGC+TGG	+	chr1.2:71248907-71248926	None:intergenic	50.0%
	AGGTGCAACATGTGTATGCC+GGG	-	chr1.2:71245550-71245569	MS.gene60726:CDS	50.0%
	ATGGGAAATGCAGCTTGTGC+TGG	-	chr1.2:71248769-71248788	MS.gene60726:intron	50.0%
	GAGGTGCAACATGTGTATGC+CGG	-	chr1.2:71245549-71245568	MS.gene60726:CDS	50.0%
	GATGAGAATAATGGCCCTCC+GGG	+	chr1.2:71245484-71245503	None:intergenic	50.0%
	GCAAAGGCAATGGTGAGTGA+TGG	+	chr1.2:71248853-71248872	None:intergenic	50.0%
	GCAGATTGGAGCAGAGTTTG+TGG	-	chr1.2:71248672-71248691	MS.gene60726:intron	50.0%
	GCTTGTCCAACGCTAACAGT+AGG	+	chr1.2:71249003-71249022	None:intergenic	50.0%
	GTGAGTCATTTGCAATGGGG+AGG	-	chr1.2:71245530-71245549	MS.gene60726:CDS	50.0%
	TAATTTCCTGCTGCAACGGC+TGG	+	chr1.2:71249766-71249785	None:intergenic	50.0%
	TACCATCCTTTCCTCTCTGG+CGG	-	chr1.2:71248967-71248986	MS.gene60726:intron	50.0%
	TTCATGGAACCACCGCTTGT+TGG	+	chr1.2:71249724-71249743	None:intergenic	50.0%
!!	ATGCAGGTTGGTGAATTGGC+TGG	-	chr1.2:71249298-71249317	MS.gene60726:intron	50.0%
!!	TGCGCTTGTTATGCGCTCAA+AGG	-	chr1.2:71248940-71248959	MS.gene60726:intron	50.0%
	AAGCTCCTAACCGGTTGAGG+TGG	+	chr1.2:71249886-71249905	None:intergenic	55.0%
	AATGCTAGGCCAACAAGCGG+TGG	-	chr1.2:71249712-71249731	MS.gene60726:CDS	55.0%
	ATGAATCCGGTGCGCACCTT+AGG	-	chr1.2:71249739-71249758	MS.gene60726:CDS	55.0%
	CTGAACCACCTCAACCGGTT+AGG	-	chr1.2:71249878-71249897	MS.gene60726:CDS	55.0%
	GAAATGGCGAAACGCCGCAA+AGG	+	chr1.2:71248869-71248888	None:intergenic	55.0%
	GAACATGTGCCCACGGGAAA+TGG	+	chr1.2:71248885-71248904	None:intergenic	55.0%
	TAAGGTGCGCACCGGATTCA+TGG	+	chr1.2:71249740-71249759	None:intergenic	55.0%
	TAGCGACGGAAGCTCCTAAC+CGG	+	chr1.2:71249895-71249914	None:intergenic	55.0%
	TAGGCTGGAACATGTGCCCA+CGG	+	chr1.2:71248892-71248911	None:intergenic	55.0%
	TGAACGTACCGCACGGGAAT+CGG	+	chr1.2:71249085-71249104	None:intergenic	55.0%
	TGCAACATGTGTATGCCGGG+TGG	-	chr1.2:71245553-71245572	MS.gene60726:CDS	55.0%
!!	CTTGGTGCTCTAGCTGGTTC+TGG	-	chr1.2:71249820-71249839	MS.gene60726:CDS	55.0%
!!	TATTTGGTGGCACCGACGCT+TGG	-	chr1.2:71249802-71249821	MS.gene60726:CDS	55.0%
!!	TGTTGCTACCGATTCCCGTG+CGG	-	chr1.2:71249074-71249093	MS.gene60726:intron	55.0%
	AGGCTGGAACATGTGCCCAC+GGG	+	chr1.2:71248891-71248910	None:intergenic	60.0%
	ATCGGAGTCAGGGACACCGA+CGG	-	chr1.2:71245429-71245448	MS.gene60726:CDS	60.0%
	CACCGCCAGAGAGGAAAGGA+TGG	+	chr1.2:71248972-71248991	None:intergenic	60.0%
	CGGAAGCTCCTAACCGGTTG+AGG	+	chr1.2:71249889-71249908	None:intergenic	60.0%
	CGGAGCTGAACCACCTCAAC+CGG	-	chr1.2:71249873-71249892	MS.gene60726:CDS	60.0%
	GCGAAACGCCGCAAAGGCAA+TGG	+	chr1.2:71248863-71248882	None:intergenic	60.0%
	GGCGTTTCGCCATTTCCCGT+GGG	-	chr1.2:71248873-71248892	MS.gene60726:intron	60.0%
	GGTGTCCCTGACTCCGATTC+TGG	+	chr1.2:71245427-71245446	None:intergenic	60.0%
	GTGACACCGCCAGAGAGGAA+AGG	+	chr1.2:71248976-71248995	None:intergenic	60.0%
	TGCTGCAACGGCTGGACCTA+AGG	+	chr1.2:71249758-71249777	None:intergenic	60.0%
	TTAGGTCCAGCCGTTGCAGC+AGG	-	chr1.2:71249757-71249776	MS.gene60726:CDS	60.0%
!	CCAGCTAGAGCACCAAGCGT+CGG	+	chr1.2:71249817-71249836	None:intergenic	60.0%
!!	AATGGCCCTCCGGGAGTATC+CGG	+	chr1.2:71245475-71245494	None:intergenic	60.0%
	CGACACCGGATACTCCCGGA+GGG	-	chr1.2:71245467-71245486	MS.gene60726:CDS	65.0%
	CGGCGTTTCGCCATTTCCCG+TGG	-	chr1.2:71248872-71248891	MS.gene60726:intron	65.0%
	GCACAGTGACACCGCCAGAG+AGG	+	chr1.2:71248981-71249000	None:intergenic	65.0%
	GCTGGACCTAAGGTGCGCAC+CGG	+	chr1.2:71249748-71249767	None:intergenic	65.0%
!!	CCGACGCTTGGTGCTCTAGC+TGG	-	chr1.2:71249814-71249833	MS.gene60726:CDS	65.0%
	AGGGACACCGACGGCAGCGT+CGG	-	chr1.2:71245438-71245457	MS.gene60726:CDS	70.0%
	CCGGCGACACCGGATACTCC+CGG	-	chr1.2:71245463-71245482	MS.gene60726:CDS	70.0%
	GCGACACCGGATACTCCCGG+AGG	-	chr1.2:71245466-71245485	MS.gene60726:CDS	70.0%
!!	CCGGGAGTATCCGGTGTCGC+CGG	+	chr1.2:71245466-71245485	None:intergenic	70.0%
!!	ACCGACGGCAGCGTCGGTGC+CGG	-	chr1.2:71245444-71245463	MS.gene60726:CDS	75.0%
!!	AGCGTCGGTGCCGGCGACAC+CGG	-	chr1.2:71245453-71245472	MS.gene60726:CDS	75.0%
!!!	GCCGGCACCGACGCTGCCGT+CGG	+	chr1.2:71245448-71245467	None:intergenic	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	71245421	71249915	71245421	ID=MS.gene60726
chr1.2	mRNA	71245421	71249915	71245421	ID=MS.gene60726.t1;Parent=MS.gene60726
chr1.2	exon	71249691	71249915	71249691	ID=MS.gene60726.t1.exon1;Parent=MS.gene60726.t1
chr1.2	CDS	71249691	71249915	71249691	ID=cds.MS.gene60726.t1;Parent=MS.gene60726.t1
chr1.2	exon	71246241	71246660	71246241	ID=MS.gene60726.t1.exon2;Parent=MS.gene60726.t1
chr1.2	CDS	71246241	71246660	71246241	ID=cds.MS.gene60726.t1;Parent=MS.gene60726.t1
chr1.2	exon	71245971	71246032	71245971	ID=MS.gene60726.t1.exon3;Parent=MS.gene60726.t1
chr1.2	CDS	71245971	71246032	71245971	ID=cds.MS.gene60726.t1;Parent=MS.gene60726.t1
chr1.2	exon	71245421	71245616	71245421	ID=MS.gene60726.t1.exon4;Parent=MS.gene60726.t1
chr1.2	CDS	71245421	71245616	71245421	ID=cds.MS.gene60726.t1;Parent=MS.gene60726.t1

Gene Sequence

>MS.gene60726

ATGCCAGAATCGGAGTCAGGGACACCGACGGCAGCGTCGGTGCCGGCGACACCGGATACTCCCGGAGGGCCATTATTCTCATCTATAAGAATTGATTCACTTGATCAACGTGAGTCATTTGCAATGGGGAGGTGCAACATGTGTATGCCGGGTGGTAAAAGCAATAGTTGCATCGCTAATTTCTCTGCTGGAGTTCGAATTCCAAGTGTCTCTCTTTCTCAGAAGGTAACATCTCTCTCTTTATGATTTTTGGTCAACGAATTTTGGTGAATTATTTTTATGAATATTTTTTTCTCATACCACCGCCCACAAATTAATTCCATTTGGTTTCTAGCTTGCATGGTCAGATTTAACTGTTTCATGGGAGTTATTTATTATTTAGAAGACAAAATAACAGTAAACCTTGACTAAAATATCAAATAGATAGCATTTTTTTTAGGGAATAGATAATGTTATATACTATTAGTACTATATGTATGTGACATAAATGTATTAGATATTAATTAATATTAGTTAATTTAATTAACAATTGGGCATTACCGCTTGTTTTTGTATTTGTAATGTTTTTCACAATTTGTATCTTATGATTTGATGAGATGTGCATGATTGGGATATTAGCAAAATAATGCATAAATAGTATTGTATGCAGGTGGTATACGATATTCTTTGAAGTCTTCAATATTCTTATGTATACTCTTCGCTGTCATTTTTATAAGAAATACTGTACTAAGTATTATCTTTCTTTTAATTATAAAATATATAAACTTGTTATAAAGTCTATTTATATATACATTCCAGCAAATTCCTTCAAAAAAAAATATACATTCCAGCAAATAAAATTTATTTCCTAAAAAAAAAAAGCAAACAAAAATATCATTTTCTATTGAAAAAGCCTTGAAAACAATTTTTTTTTATTAAATACAAACACCGTGTATAATATAGTATATGTACATTCTTATCTTTCTCTTACTTTTTATGTTCTACAAACACATATTTCAAAACTATGCATAAAAACTAAAGAACATAAAAAAAATAAAGAATGATTTCACATAATTCTATTTATATAATTGTATTAGTTAGTACTATTGTTGAAATGCATAAGAGTACATTATTATCTTTTTTATATTGAAATGAAATATATGGTTGCGAGCAGTTATCTTCAGTATTCACACATTTCAATATATAATTTTGTCGGCCTTCTAATGTATTTTGACATAGATGGGTTATTGGATAAAAACAATTTCATTGATAAACAAAAGATGGATTACGACTTCTGAGTACATAGACTTGAAACAGTGCAAAAACATGTAGGATATTAAGCTACACTGGTTATCTTGGCTTGATTGTAAAAAACGTACTATATATCTGTCGCTAGCAAGAGCCCTAATAAACCCATGTTACTAGTTGCATTTGTTGAAAGAAACTTTTCTAAAGTAAACTTGTGAAAATCTTATTTCCGAAAGTATATTGAATAGTTTTACTTTATAAATGTGAACGAGTTGTTAATGATTTTGCAAGAAATAGAATGCAAGAAATATATTATTCAAATGAGATTTCTCTTTGTAAAATAAAAAAGATTAATGCAGACGATTTACTTAACTCGTACTTCCTCCGGTCACTATTATAAGCAAAAATTGATTTTTTAGGTTCATTCAATAAATGATGTATGTAGTCTATATATATATATATATACTACATACATCATTTATTGAATGAATCTAAAAAGTGAACTTTTGCTTATAATAGTGATCGGAGGAAGTATATGACAGCCAAAATTTCAAGGACGGACATGTTTTCTCAATATCAAGGAGGTATATATTTTACTAAGGGTGTGTGGATGGAGAGTTTAAGAGAGGATGGGAGGGGAGAGTTTACTTTTCGTTTTTAAATTACGAACACTATGAAATATTTTTTTTTAAATTAAAATAACAACATGATAAACGATCATATAATACTTCAATCACACTTAATTTATTATAGAAAAACATGTTATATAGTCCGTATTTTAAAAAAGAAACGTAAATCATCCCCTTCCTTTTCCCTCATAAACTCTCAATCCAAATACACCCTAAGAAAATATCAAACATGTTCGATGTTTTTTTTTTTAAAAAAAAAAGTTGATTAAATGAGGGGTAGGATAAGTGTAAAAGAATTGAGCCGAATACTTAAACTTTGAGTTTGACTTGTGAAAAAATGAATAATCTGAGCTTGACTCATTTATTTAATCGTGTCAATCTGAAAACTTGGGTTTGGATTTATTTTTTTTGAAAGGAACTTGGGTTCGACTTTTTTTTAAGGGAATGAACTTGGGTTTGACTTGTTTTTGCAATTATGCACATTTTGACTATGATAAAAAATAAAATTAAAAATTTTTAAAAAAAAAAGTTAGGTTAACAAGTGTCATAAGGGCATTGTTTGAGGAATTCATTATAAAAAATTTTGTTTTGAAAATATAAATCAAACACATGTAAAAGTTATAATTACACAAGTTGTTATATAAAAGTTACCACTTTTAGTTGTTTAAACAATGCTCTAAAAACATTTGTTAGCATTTCCTAAAAATAAATGTAAAATAAAAAGTTAAAAGACATTCATTCTTTTGCTACTAATATTTTGAAATTACATAGATTTTTTTTGGACTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATGTTTGACTTGATTTATTAAAGTTTCTTAACTGCCATAAATACTTTGAGACTGTTTGAAAAAATTTATACAACAAAATATGACTTTGACCAAAAAAAAACAAACAACTTATAACATATCTATGACGTATTTTCAACTTTTTTTTTTCAAAAGCTCTCCAAACAAAGGTTAACCATGTAGTTGATTTAATTTTGTAAGCTTTGGGGTTAGAATGATGATGTGTGTACATAGGTTTATTGATGTGCTACTTTTCTACTTATAGCATTGCTTGAGTTATTAGTGGTGATTACTAAAAAAAAATAAAGAAAAAATGTATTTTTGAATACACATGGAATGTTCAAAATGTAGATTTTTCCAAAAATCTACATTTGGAACATTCCAATAAACTCCAAAGTAGCTCTTTCATTCTACTATATAAGCATCGAATTTTCAAAAACTCTTCTAAAATATTATCCATTGTCACATTATTCTAATCCCCCTTAATGAAAAAGGAAAAGTAAAATTAATATTGATTTTTCAATGTTAAAATGTGTCGCAGATTGGAGCAGAGTTTGTGGGAACATTCATACTAATATATGCAGCAACAGCAGGACCAATAGTGAACAACAAATACAATGGAGTAGAAACTCTTATGGGAAATGCAGCTTGTGCTGGACTAACAGTCATGTTCATTATTCTCTCAATTGGTCATATCTCAGGTGCACATCTCAATCCATCACTCACCATTGCCTTTGCGGCGTTTCGCCATTTCCCGTGGGCACATGTTCCAGCCTACATTGCAGCACAAGTCTCTGCTTCGATCTGCGCTTGTTATGCGCTCAAAGGAGTTTACCATCCTTTCCTCTCTGGCGGTGTCACTGTGCCTACTGTTAGCGTTGGACAAGCTTTTGCAACTGAGTTTATCATCACTTTTATTCTCTTGTTTGTTGTTACTGCTGTTGCTACCGATTCCCGTGCGGTACGTTCAATTCATTCATTTGTTTGTGTTATCTTTTTTAACTTCATTGATCATCTTTTCTAACTGCGTTGACCACTTAAAAAGTTGCCGCGAAATTTCAAGTTCTCTAAATTGGGTTGAACAAAAAATAATTTATCTTGAATTATTGAGTTTTTTATGAGTTAAGTTGTTTTAATTCATGGATTTTATTTTTGTATATGCAATGCAGGTTGGTGAATTGGCTGGTATTGCTGTTGGTGCTACAGTTTTGCTCAACATTCTTATCTCAGGGTATGGAACCTTTTCTCATGAATTGATGTTATTGATTTGTATTTGGTTTTCATAATAGGTGTATATGTCAAATGTTATATTCAGTTGGAATTCAATGATAATGTTTCAAAGGAATTAATCATGAAAAACTATTCCTAAGGTTTTTAATGACATGACTGTGTCCATCAAGTTAGTCTCCGAATATAAATTTTGTCTGAATAATGACAGGATCAGGAACTTAATTTAATCATTTATGAATTAATATGACTGATGCATTTTATTTTTTCGTAGAATAATTTGATTCTATTGTTTACATGATTTGATTTGAGTTCTTTATTCTAAGATTGTGAGTAATGTTGTGGTTGTAAATGCTAGGCCAACAAGCGGTGGTTCCATGAATCCGGTGCGCACCTTAGGTCCAGCCGTTGCAGCAGGAAATTACAAGCATCTCTGGATATATTTGGTGGCACCGACGCTTGGTGCTCTAGCTGGTTCTGGAGTTTATACCCTTATCAAGCTTCGTGATAACGGAGCTGAACCACCTCAACCGGTTAGGAGCTTCCGTCGCTAG

Protein sequence

>MS.gene60726.t1

MPESESGTPTAASVPATPDTPGGPLFSSIRIDSLDQRESFAMGRCNMCMPGGKSNSCIANFSAGVRIPSVSLSQKIGAEFVGTFILIYAATAGPIVNNKYNGVETLMGNAACAGLTVMFIILSIGHISGAHLNPSLTIAFAAFRHFPWAHVPAYIAAQVSASICACYALKGVYHPFLSGGVTVPTVSVGQAFATEFIITFILLFVVTAVATDSRAVGELAGIAVGATVLLNILISGPTSGGSMNPVRTLGPAVAAGNYKHLWIYLVAPTLGALAGSGVYTLIKLRDNGAEPPQPVRSFRR