AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65833


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65833.t1 MTR_3g019090 93.194 191 13 0 1 191 1 191 1.72e-133 372
MS.gene65833.t1 MTR_3g019160 76.440 191 45 0 1 191 1 191 6.40e-108 307
MS.gene65833.t1 MTR_3g019120 77.320 194 37 2 1 189 1 192 4.19e-105 307
MS.gene65833.t1 MTR_3g019120 77.320 194 37 2 1 189 1 192 1.17e-103 307
MS.gene65833.t1 MTR_3g019150 72.251 191 52 1 1 191 1 190 1.37e-102 293
MS.gene65833.t1 MTR_8g039090 69.474 190 58 0 2 191 3 192 2.54e-101 290
MS.gene65833.t1 MTR_3g019090 95.070 142 7 0 50 191 1 142 1.28e-99 284
MS.gene65833.t1 MTR_3g019120 83.784 148 24 0 42 189 5 152 5.75e-88 266
MS.gene65833.t1 MTR_3g019150 71.287 101 29 0 91 191 34 134 6.55e-51 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65833.t1 AT3G27890 68.984 187 58 0 4 190 10 196 7.60e-92 266

Find 42 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTCTGCCTTGAAGTTTA+AGG 0.117582 3.2:+9572227 None:intergenic
AATCGACTATATTGATATTT+CGG 0.315855 3.2:-9572751 MS.gene65833:CDS
AAATATCAATATAGTCGATT+TGG 0.331349 3.2:+9572754 None:intergenic
ATTTCTGCCTTGAAGTTTAA+GGG 0.343293 3.2:+9572228 None:intergenic
AACATCATCTTCGCCAAATC+GGG 0.347815 3.2:-9572399 MS.gene65833:CDS
CATACTGGCCTCATTCGTTA+TGG 0.349761 3.2:-9573349 MS.gene65833:intron
CGCTAAAGAATGCGATTGAT+TGG 0.357043 3.2:-9572504 MS.gene65833:CDS
GGTTTACCAGCCCATGCATT+TGG 0.360432 3.2:+9572469 None:intergenic
ATTAAGATTGTTGCTCTCTC+TGG 0.362873 3.2:-9573394 MS.gene65833:CDS
CAACATCATCTTCGCCAAAT+CGG 0.377634 3.2:-9572400 MS.gene65833:CDS
GGCTCCTCCAACACTTACTA+TGG 0.391189 3.2:+9572441 None:intergenic
TATCAAGGAAAACCCCGATT+TGG 0.397239 3.2:+9572386 None:intergenic
CTGGATGCTTAAATACATCT+AGG 0.405336 3.2:+9572335 None:intergenic
CGAAAAGCTTCAACTTCAGC+TGG 0.413282 3.2:+9572684 None:intergenic
GAGACAGAATAATTATATTC+AGG 0.417383 3.2:+9572621 None:intergenic
GTAAGTGTTGGAGGAGCCTT+TGG 0.428342 3.2:-9572436 MS.gene65833:CDS
TTTAAGGGTAAATTCCTTCA+AGG 0.429920 3.2:+9572243 None:intergenic
TGAAGAGATCAAGAATAGAT+TGG 0.437949 3.2:-9572281 MS.gene65833:CDS
GAATATAATTATTCTGTCTC+AGG 0.442769 3.2:-9572618 MS.gene65833:intron
GAAAGTTCTTGTGTCCTTGA+AGG 0.447891 3.2:-9572257 MS.gene65833:CDS
TATTAATGAAATGAATATCA+AGG 0.449069 3.2:+9572371 None:intergenic
GCTAAAGAATGCGATTGATT+GGG 0.485585 3.2:-9572503 MS.gene65833:CDS
GAATTTACCCTTAAACTTCA+AGG 0.490730 3.2:-9572235 MS.gene65833:CDS
TTACCAGCCCATGCATTTGG+TGG 0.503471 3.2:+9572472 None:intergenic
AACTCTGATCTTGAGAAAGA+AGG 0.504804 3.2:-9572714 MS.gene65833:CDS
CAATCAATCGCATTCTTTAG+CGG 0.506871 3.2:+9572505 None:intergenic
GTTGGAGGAGCCTTTGGTGG+AGG 0.513008 3.2:-9572430 MS.gene65833:CDS
CATCAAGACCACCAAATGCA+TGG 0.519270 3.2:-9572480 MS.gene65833:CDS
GTAAATTACCATAACGAATG+AGG 0.533048 3.2:+9573341 None:intergenic
AGTGTTGGAGGAGCCTTTGG+TGG 0.533206 3.2:-9572433 MS.gene65833:CDS
TCAAGATCAGAGTTCAACAT+TGG 0.545865 3.2:+9572723 None:intergenic
GCTGCCATAGTAAGTGTTGG+AGG 0.554560 3.2:-9572445 MS.gene65833:CDS
AGACCACCAAATGCATGGGC+TGG 0.561726 3.2:-9572475 MS.gene65833:CDS
CCAACACTTACTATGGCAGC+AGG 0.570215 3.2:+9572448 None:intergenic
ATCAAGACCACCAAATGCAT+GGG 0.580578 3.2:-9572479 MS.gene65833:CDS
CCTGCTGCCATAGTAAGTGT+TGG 0.586831 3.2:-9572448 MS.gene65833:CDS
AATGAGGCCAGTATGAAAAG+AGG 0.609156 3.2:+9573357 None:intergenic
AATACATCTAGGTAGAACTC+AGG 0.627908 3.2:+9572346 None:intergenic
ACATCATCTTCGCCAAATCG+GGG 0.637712 3.2:-9572398 MS.gene65833:CDS
TTGATTAACAAATAATGACA+AGG 0.643119 3.2:-9573420 None:intergenic
ATGTAACGATGATGGTGACT+TGG 0.649079 3.2:-9572308 MS.gene65833:CDS
TGATTAACAAATAATGACAA+GGG 0.676333 3.2:-9573419 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTAGCAAAAAAAAAAAAA+AGG - chr3.2:9572513-9572532 MS.gene65833:intron 15.0%
!! TATTAATGAAATGAATATCA+AGG + chr3.2:9573263-9573282 None:intergenic 15.0%
!! TCTTTGAATATTAAATTTGA+TGG - chr3.2:9572790-9572809 MS.gene65833:CDS 15.0%
!!! ACATTTTTTTTTCTATTCAT+TGG - chr3.2:9572817-9572836 MS.gene65833:intron 15.0%
!!! TTTAACTTGTTGAAAAAATA+GGG - chr3.2:9572716-9572735 MS.gene65833:CDS 15.0%
!! AAAAAGAATCTTATTGACAA+AGG + chr3.2:9573072-9573091 None:intergenic 20.0%
!! AAATATCAATATAGTCGATT+TGG + chr3.2:9572880-9572899 None:intergenic 20.0%
!! AATCGACTATATTGATATTT+CGG - chr3.2:9572880-9572899 MS.gene65833:intron 20.0%
!! ATATTGCATATTATATACAG+GGG - chr3.2:9572422-9572441 MS.gene65833:CDS 20.0%
!! GATATTGCATATTATATACA+GGG - chr3.2:9572421-9572440 MS.gene65833:CDS 20.0%
!!! ATATTTTAAATGTAGAGAAG+TGG + chr3.2:9572472-9572491 None:intergenic 20.0%
!!! GTTTAACTTGTTGAAAAAAT+AGG - chr3.2:9572715-9572734 MS.gene65833:CDS 20.0%
! AAAAGTTGAAATTAACCTGT+CGG - chr3.2:9572661-9572680 MS.gene65833:CDS 25.0%
! AAACTGAGTGAATATTGTTT+AGG - chr3.2:9572556-9572575 MS.gene65833:intron 25.0%
! AACTGAGTGAATATTGTTTA+GGG - chr3.2:9572557-9572576 MS.gene65833:intron 25.0%
! ATTCAAAGACTCAAAATCAA+AGG + chr3.2:9572779-9572798 None:intergenic 25.0%
! GAATATAATTATTCTGTCTC+AGG - chr3.2:9573013-9573032 MS.gene65833:intron 25.0%
! GAGACAGAATAATTATATTC+AGG + chr3.2:9573013-9573032 None:intergenic 25.0%
! GGATATTGCATATTATATAC+AGG - chr3.2:9572420-9572439 MS.gene65833:CDS 25.0%
! TATTGCATATTATATACAGG+GGG - chr3.2:9572423-9572442 MS.gene65833:CDS 25.0%
!! TGAGTTTAGTTTAATTGGTA+GGG - chr3.2:9572399-9572418 MS.gene65833:CDS 25.0%
AAGGAAAAAAAGAAGAGAAG+AGG + chr3.2:9572344-9572363 None:intergenic 30.0%
AATTTCTGCCTTGAAGTTTA+AGG + chr3.2:9573407-9573426 None:intergenic 30.0%
ACCAATTAAACTAAACTCAC+CGG + chr3.2:9572398-9572417 None:intergenic 30.0%
ACTGAGTGAATATTGTTTAG+GGG - chr3.2:9572558-9572577 MS.gene65833:intron 30.0%
ATATTATATACAGGGGGTTA+GGG - chr3.2:9572429-9572448 MS.gene65833:CDS 30.0%
ATTTCTGCCTTGAAGTTTAA+GGG + chr3.2:9573406-9573425 None:intergenic 30.0%
CTAAGCAAAAATGTTCTTGA+TGG - chr3.2:9572851-9572870 MS.gene65833:intron 30.0%
GAATTTACCCTTAAACTTCA+AGG - chr3.2:9573396-9573415 MS.gene65833:CDS 30.0%
GTAAATTACCATAACGAATG+AGG + chr3.2:9572293-9572312 None:intergenic 30.0%
TGAAGAGATCAAGAATAGAT+TGG - chr3.2:9573350-9573369 MS.gene65833:CDS 30.0%
TTTAAGGGTAAATTCCTTCA+AGG + chr3.2:9573391-9573410 None:intergenic 30.0%
! ACTTTTCCAACCTAAAATTG+CGG + chr3.2:9572647-9572666 None:intergenic 30.0%
! CTATTAAAACCCGCAATTTT+AGG - chr3.2:9572634-9572653 MS.gene65833:CDS 30.0%
! GTGAGTTTAGTTTAATTGGT+AGG - chr3.2:9572398-9572417 MS.gene65833:CDS 30.0%
! TTTTCAACAAGTTAAACGAC+TGG + chr3.2:9572712-9572731 None:intergenic 30.0%
!! AACTTGTTGAAAAAATAGGG+CGG - chr3.2:9572719-9572738 MS.gene65833:CDS 30.0%
!! CTAAAATTGCGGGTTTTAAT+AGG + chr3.2:9572636-9572655 None:intergenic 30.0%
AACTCTGATCTTGAGAAAGA+AGG - chr3.2:9572917-9572936 MS.gene65833:intron 35.0%
AATACATCTAGGTAGAACTC+AGG + chr3.2:9573288-9573307 None:intergenic 35.0%
ATTAAGATTGTTGCTCTCTC+TGG - chr3.2:9572237-9572256 MS.gene65833:CDS 35.0%
CAATCAATCGCATTCTTTAG+CGG + chr3.2:9573129-9573148 None:intergenic 35.0%
CATATTATATACAGGGGGTT+AGG - chr3.2:9572428-9572447 MS.gene65833:CDS 35.0%
CCAATTAAACTAAACTCACC+GGG + chr3.2:9572397-9572416 None:intergenic 35.0%
CCAGAAAAATGTAACGATGA+TGG - chr3.2:9573315-9573334 MS.gene65833:intron 35.0%
CTCGAAGTAGTCATTTAGAA+AGG + chr3.2:9572363-9572382 None:intergenic 35.0%
CTGAGTGAATATTGTTTAGG+GGG - chr3.2:9572559-9572578 MS.gene65833:intron 35.0%
CTGGATGCTTAAATACATCT+AGG + chr3.2:9573299-9573318 None:intergenic 35.0%
CTTTCTAAATGACTACTTCG+AGG - chr3.2:9572361-9572380 MS.gene65833:CDS 35.0%
GCTAAAGAATGCGATTGATT+GGG - chr3.2:9573128-9573147 MS.gene65833:intron 35.0%
GTTTAATAAACCTATCCGAC+AGG + chr3.2:9572679-9572698 None:intergenic 35.0%
TCAAGATCAGAGTTCAACAT+TGG + chr3.2:9572911-9572930 None:intergenic 35.0%
TTGAAATTAACCTGTCGGAT+AGG - chr3.2:9572666-9572685 MS.gene65833:CDS 35.0%
! CCATCATCGTTACATTTTTC+TGG + chr3.2:9573318-9573337 None:intergenic 35.0%
! CTTTTCCAACCTAAAATTGC+GGG + chr3.2:9572646-9572665 None:intergenic 35.0%
!! AATTGCGGGTTTTAATAGGT+CGG + chr3.2:9572632-9572651 None:intergenic 35.0%
!! TAAAACCCGCAATTTTAGGT+TGG - chr3.2:9572638-9572657 MS.gene65833:CDS 35.0%
AACATCATCTTCGCCAAATC+GGG - chr3.2:9573232-9573251 MS.gene65833:intron 40.0%
AATGAGGCCAGTATGAAAAG+AGG + chr3.2:9572277-9572296 None:intergenic 40.0%
ATCAAGACCACCAAATGCAT+GGG - chr3.2:9573152-9573171 MS.gene65833:intron 40.0%
CAACATCATCTTCGCCAAAT+CGG - chr3.2:9573231-9573250 MS.gene65833:intron 40.0%
CGCTAAAGAATGCGATTGAT+TGG - chr3.2:9573127-9573146 MS.gene65833:intron 40.0%
GAAAGTTCTTGTGTCCTTGA+AGG - chr3.2:9573374-9573393 MS.gene65833:CDS 40.0%
TATCAAGGAAAACCCCGATT+TGG + chr3.2:9573248-9573267 None:intergenic 40.0%
TTGTTGAAAAAATAGGGCGG+TGG - chr3.2:9572722-9572741 MS.gene65833:CDS 40.0%
! ATGTAACGATGATGGTGACT+TGG - chr3.2:9573323-9573342 MS.gene65833:intron 40.0%
! CCCGGTGAGTTTAGTTTAAT+TGG - chr3.2:9572394-9572413 MS.gene65833:CDS 40.0%
! CGAAAAGCCTCTTTTCATAC+TGG - chr3.2:9572267-9572286 MS.gene65833:CDS 40.0%
ACATCATCTTCGCCAAATCG+GGG - chr3.2:9573233-9573252 MS.gene65833:intron 45.0%
CATCAAGACCACCAAATGCA+TGG - chr3.2:9573151-9573170 MS.gene65833:intron 45.0%
CGAAAAGCTTCAACTTCAGC+TGG + chr3.2:9572950-9572969 None:intergenic 45.0%
CTAAGTAGGGGTGGCAAAAT+AGG - chr3.2:9572592-9572611 MS.gene65833:intron 45.0%
CTTCGAGGCTAATCAAATCC+CGG - chr3.2:9572376-9572395 MS.gene65833:CDS 45.0%
GGAGAGAAGACACTAAGTAG+GGG - chr3.2:9572580-9572599 MS.gene65833:intron 45.0%
GGGAGAGAAGACACTAAGTA+GGG - chr3.2:9572579-9572598 MS.gene65833:intron 45.0%
! CATACTGGCCTCATTCGTTA+TGG - chr3.2:9572282-9572301 MS.gene65833:CDS 45.0%
! TTGAGCTTTTCCTCCACCAA+AGG + chr3.2:9573214-9573233 None:intergenic 45.0%
CAAAATAGGTCGAGCCTGTC+GGG - chr3.2:9572606-9572625 MS.gene65833:intron 50.0%
CCAACACTTACTATGGCAGC+AGG + chr3.2:9573186-9573205 None:intergenic 50.0%
CCTGCTGCCATAGTAAGTGT+TGG - chr3.2:9573183-9573202 MS.gene65833:intron 50.0%
GAGAAGACACTAAGTAGGGG+TGG - chr3.2:9572583-9572602 MS.gene65833:intron 50.0%
GCAAAATAGGTCGAGCCTGT+CGG - chr3.2:9572605-9572624 MS.gene65833:intron 50.0%
GGCTCCTCCAACACTTACTA+TGG + chr3.2:9573193-9573212 None:intergenic 50.0%
GGGGAGAGAAGACACTAAGT+AGG - chr3.2:9572578-9572597 MS.gene65833:intron 50.0%
GGTTTACCAGCCCATGCATT+TGG + chr3.2:9573165-9573184 None:intergenic 50.0%
TGAAAAAATAGGGCGGTGGC+AGG - chr3.2:9572726-9572745 MS.gene65833:CDS 50.0%
TTACCAGCCCATGCATTTGG+TGG + chr3.2:9573162-9573181 None:intergenic 50.0%
! TTTTAATAGGTCGGCCCGAC+AGG + chr3.2:9572623-9572642 None:intergenic 50.0%
!! GCTGCCATAGTAAGTGTTGG+AGG - chr3.2:9573186-9573205 MS.gene65833:intron 50.0%
!! GTAAGTGTTGGAGGAGCCTT+TGG - chr3.2:9573195-9573214 MS.gene65833:intron 50.0%
AGACCACCAAATGCATGGGC+TGG - chr3.2:9573156-9573175 MS.gene65833:intron 55.0%
! AGTGTTGGAGGAGCCTTTGG+TGG - chr3.2:9573198-9573217 MS.gene65833:intron 55.0%
GGGGGTTAGGGTTCGAAGCT+CGG - chr3.2:9572441-9572460 MS.gene65833:CDS 60.0%
GTTGGAGGAGCCTTTGGTGG+AGG - chr3.2:9573201-9573220 MS.gene65833:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 9572225 9573428 9572225 ID=MS.gene65833
chr3.2 mRNA 9572225 9573428 9572225 ID=MS.gene65833.t1;Parent=MS.gene65833
chr3.2 exon 9573350 9573428 9573350 ID=MS.gene65833.t1.exon1;Parent=MS.gene65833.t1
chr3.2 CDS 9573350 9573428 9573350 ID=cds.MS.gene65833.t1;Parent=MS.gene65833.t1
chr3.2 exon 9572619 9572810 9572619 ID=MS.gene65833.t1.exon2;Parent=MS.gene65833.t1
chr3.2 CDS 9572619 9572810 9572619 ID=cds.MS.gene65833.t1;Parent=MS.gene65833.t1
chr3.2 exon 9572225 9572529 9572225 ID=MS.gene65833.t1.exon3;Parent=MS.gene65833.t1
chr3.2 CDS 9572225 9572529 9572225 ID=cds.MS.gene65833.t1;Parent=MS.gene65833.t1
Gene Sequence

>MS.gene65833

ATGACAAGGGTGATTAAGATTGTTGCTCTCTCTGGTTCTCTTCGAAAAGCCTCTTTTCATACTGGCCTCATTCGTTATGGTAATTTACTTCTTATTCAAACACTAATTCTTCTCTTCCTCTTCTCTTCTTTTTTTCCTTTCTAAATGACTACTTCGAGGCTAATCAAATCCCGGTGAGTTTAGTTTAATTGGTAGGGATATTGCATATTATATACAGGGGGTTAGGGTTCGAAGCTCGGACACTCCACTTCTCTACATTTAAAATATGTGAAATTTAGCAAATAGACTACTTAGCAAAAAAAAAAAAAAGGCAAAATGTAAAATAAAAAATAAACTGAGTGAATATTGTTTAGGGGGAGAGAAGACACTAAGTAGGGGTGGCAAAATAGGTCGAGCCTGTCGGGCCGACCTATTAAAACCCGCAATTTTAGGTTGGAAAAGTTGAAATTAACCTGTCGGATAGGTTTATTAAACTCAAAATTAACCAGTCGTTTAACTTGTTGAAAAAATAGGGCGGTGGCAGGACGATTTGACATAAGTAGATGAATTAACCTTTGATTTTGAGTCTTTGAATATTAAATTTGATGGTTTTACATTTTTTTTTCTATTCATTGGTAGCTATTGAGCTAAGCAAAAATGTTCTTGATGGTATCCAAATCGACTATATTGATATTTCGGACTTGCCAATGTTGAACTCTGATCTTGAGAAAGAAGGAACTTTTCCAGCTGAAGTTGAAGCTTTTCGTCAAAAGATTCTTGCAGCTGATAGTGTTCTCTTTGCTTCTCCTGAATATAATTATTCTGTCTCAGGTAAGTAAACATCATGTTGTTTTTTTCTTTATAACCTTTGTCAATAAGATTCTTTTTGCTAATTTATTATTTTCTCGCATTTTTTTCAGCTCCGCTAAAGAATGCGATTGATTGGGCATCAAGACCACCAAATGCATGGGCTGGTAAACCTGCTGCCATAGTAAGTGTTGGAGGAGCCTTTGGTGGAGGAAAAGCTCAACATCATCTTCGCCAAATCGGGGTTTTCCTTGATATTCATTTCATTAATAAACCTGAGTTCTACCTAGATGTATTTAAGCATCCAGAAAAATGTAACGATGATGGTGACTTGGTTGATGAAGAGATCAAGAATAGATTGGAGAAAGTTCTTGTGTCCTTGAAGGAATTTACCCTTAAACTTCAAGGCAGAAATTGA

Protein sequence

>MS.gene65833.t1

MTRVIKIVALSGSLRKASFHTGLIRYAIELSKNVLDGIQIDYIDISDLPMLNSDLEKEGTFPAEVEAFRQKILAADSVLFASPEYNYSVSAPLKNAIDWASRPPNAWAGKPAAIVSVGGAFGGGKAQHHLRQIGVFLDIHFINKPEFYLDVFKHPEKCNDDGDLVDEEIKNRLEKVLVSLKEFTLKLQGRN