Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67054.t1 | XP_013461885.1 | 97.9 | 140 | 3 | 0 | 1 | 140 | 1 | 140 | 5.90E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67054.t1 | Q9SN73 | 50.6 | 81 | 40 | 0 | 60 | 140 | 56 | 136 | 2.0e-12 | 73.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67054.t1 | A0A072VCQ1 | 97.9 | 140 | 3 | 0 | 1 | 140 | 1 | 140 | 4.3e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050076 | MS.gene67054 | 0.822366 | 2.46E-53 | -1.69E-46 |
| MS.gene050404 | MS.gene67054 | 0.875429 | 3.10E-68 | -1.69E-46 |
| MS.gene050406 | MS.gene67054 | 0.868296 | 7.26E-66 | -1.69E-46 |
| MS.gene050464 | MS.gene67054 | 0.805755 | 1.15E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene67054 | 0.835386 | 1.73E-56 | -1.69E-46 |
| MS.gene050812 | MS.gene67054 | 0.806378 | 8.47E-50 | -1.69E-46 |
| MS.gene051171 | MS.gene67054 | 0.876172 | 1.73E-68 | -1.69E-46 |
| MS.gene051240 | MS.gene67054 | 0.841779 | 3.86E-58 | -1.69E-46 |
| MS.gene051242 | MS.gene67054 | 0.879732 | 9.80E-70 | -1.69E-46 |
| MS.gene051244 | MS.gene67054 | 0.847687 | 9.88E-60 | -1.69E-46 |
| MS.gene051471 | MS.gene67054 | 0.809797 | 1.58E-50 | -1.69E-46 |
| MS.gene051582 | MS.gene67054 | 0.833591 | 4.88E-56 | -1.69E-46 |
| MS.gene052078 | MS.gene67054 | 0.805675 | 1.19E-49 | -1.69E-46 |
| MS.gene053330 | MS.gene67054 | 0.877302 | 7.01E-69 | -1.69E-46 |
| MS.gene053907 | MS.gene67054 | 0.866937 | 1.98E-65 | -1.69E-46 |
| MS.gene053971 | MS.gene67054 | 0.912264 | 2.38E-83 | -1.69E-46 |
| MS.gene054983 | MS.gene67054 | 0.802141 | 6.46E-49 | -1.69E-46 |
| MS.gene055370 | MS.gene67054 | 0.855938 | 4.51E-62 | -1.69E-46 |
| MS.gene055428 | MS.gene67054 | 0.814833 | 1.26E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene67054 | 0.815576 | 8.61E-52 | -1.69E-46 |
| MS.gene055980 | MS.gene67054 | 0.817016 | 4.10E-52 | -1.69E-46 |
| MS.gene056305 | MS.gene67054 | 0.817776 | 2.77E-52 | -1.69E-46 |
| MS.gene056451 | MS.gene67054 | 0.854601 | 1.10E-61 | -1.69E-46 |
| MS.gene056612 | MS.gene67054 | 0.843037 | 1.79E-58 | -1.69E-46 |
| MS.gene056633 | MS.gene67054 | 0.916732 | 1.25E-85 | -1.69E-46 |
| MS.gene056664 | MS.gene67054 | 0.899449 | 1.96E-77 | -1.69E-46 |
| MS.gene05702 | MS.gene67054 | 0.828506 | 8.66E-55 | -1.69E-46 |
| MS.gene058142 | MS.gene67054 | 0.816196 | 6.26E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene67054 | 0.888642 | 4.93E-73 | -1.69E-46 |
| MS.gene058276 | MS.gene67054 | 0.866911 | 2.02E-65 | -1.69E-46 |
| MS.gene058743 | MS.gene67054 | 0.808934 | 2.43E-50 | -1.69E-46 |
| MS.gene058746 | MS.gene67054 | 0.931602 | 2.96E-94 | -1.69E-46 |
| MS.gene059894 | MS.gene67054 | 0.85185 | 6.78E-61 | -1.69E-46 |
| MS.gene060407 | MS.gene67054 | 0.852103 | 5.75E-61 | -1.69E-46 |
| MS.gene061049 | MS.gene67054 | 0.833014 | 6.80E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67054.t1 | MTR_3g107397 | 97.857 | 140 | 3 | 0 | 1 | 140 | 1 | 140 | 4.37e-99 | 280 |
| MS.gene67054.t1 | MTR_4g114180 | 52.885 | 104 | 48 | 1 | 37 | 139 | 29 | 132 | 5.56e-32 | 110 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67054.t1 | AT3G47650 | 69.136 | 81 | 25 | 0 | 60 | 140 | 56 | 136 | 3.31e-37 | 124 |
Find 35 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACATTTCAGGGTCAATTA+TGG | 0.200301 | 3.4:-93314523 | MS.gene67054:CDS |
| GTGCCTCCTGTTGGCTTTGC+GGG | 0.255239 | 3.4:-93314000 | MS.gene67054:intron |
| TCAGCACAGATGACACTATT+TGG | 0.263290 | 3.4:+93314332 | None:intergenic |
| ATACGAACCGAAAACATTTC+AGG | 0.285412 | 3.4:-93314534 | MS.gene67054:CDS |
| TGCAATGGAGCTGGATTTAT+CGG | 0.322631 | 3.4:-93313744 | MS.gene67054:CDS |
| GTTCAAACTTCTGAATGTTC+AGG | 0.329988 | 3.4:-93314580 | MS.gene67054:CDS |
| AACATTTCAGGGTCAATTAT+GGG | 0.350289 | 3.4:-93314522 | MS.gene67054:CDS |
| ATTTATCTGTGGGAGGGCCT+TGG | 0.381621 | 3.4:+93314497 | None:intergenic |
| TGCCTAACACCTTCATGTCT+TGG | 0.395804 | 3.4:-93314619 | MS.gene67054:CDS |
| GGTGCCTCCTGTTGGCTTTG+CGG | 0.447694 | 3.4:-93314001 | MS.gene67054:CDS |
| TTCGATGGACTGTTTAAAGC+TGG | 0.460757 | 3.4:-93314022 | MS.gene67054:CDS |
| TACGAACCGAAAACATTTCA+GGG | 0.468265 | 3.4:-93314533 | MS.gene67054:CDS |
| TAGGTACCCGCAAAGCCAAC+AGG | 0.473779 | 3.4:+93313994 | None:intergenic |
| TGCCAAGACATGAAGGTGTT+AGG | 0.493946 | 3.4:+93314617 | None:intergenic |
| TTAAAGCTGGTGCCTCCTGT+TGG | 0.514045 | 3.4:-93314009 | MS.gene67054:CDS |
| TGTGGGAATTGCAATGGAGC+TGG | 0.521634 | 3.4:-93313753 | MS.gene67054:CDS |
| AGGGTCAATTATGGGTTCCA+AGG | 0.522125 | 3.4:-93314514 | MS.gene67054:CDS |
| GGCAGGAAGGAGATGTTATG+TGG | 0.530714 | 3.4:-93313771 | MS.gene67054:CDS |
| TGTGCTGATTGTGATGGAAA+TGG | 0.538508 | 3.4:-93314317 | MS.gene67054:intron |
| GCAGGAAGGAGATGTTATGT+GGG | 0.542628 | 3.4:-93313770 | MS.gene67054:CDS |
| TGCTCTCAATGCAAAGGTAG+CGG | 0.551522 | 3.4:-93314064 | MS.gene67054:CDS |
| CTTGAGCACATATGACCAGT+AGG | 0.554604 | 3.4:-93313715 | None:intergenic |
| GACAAGGCAATATTTATCTG+TGG | 0.574721 | 3.4:+93314486 | None:intergenic |
| GGCAATATTTATCTGTGGGA+GGG | 0.576292 | 3.4:+93314491 | None:intergenic |
| ACAAGGCAATATTTATCTGT+GGG | 0.583163 | 3.4:+93314487 | None:intergenic |
| AGGCAAAGAGCAGATACCAT+TGG | 0.591049 | 3.4:+93314637 | None:intergenic |
| ATGTTATGTGGGAATTGCAA+TGG | 0.609174 | 3.4:-93313759 | MS.gene67054:CDS |
| AATGGAGCTGGATTTATCGG+TGG | 0.609254 | 3.4:-93313741 | MS.gene67054:CDS |
| AATGTACCTTTGCAGTGACA+AGG | 0.612316 | 3.4:+93314470 | None:intergenic |
| TTGAGCACATATGACCAGTA+GGG | 0.613108 | 3.4:-93313714 | None:intergenic |
| AGGCAATATTTATCTGTGGG+AGG | 0.615579 | 3.4:+93314490 | None:intergenic |
| ATTGTCTGCTCTCAATGCAA+AGG | 0.622596 | 3.4:-93314070 | MS.gene67054:CDS |
| GTCATCTGTGCTGATTGTGA+TGG | 0.623087 | 3.4:-93314323 | MS.gene67054:CDS |
| ATATTGCCTTGTCACTGCAA+AGG | 0.634181 | 3.4:-93314476 | MS.gene67054:intron |
| GTACCCGCAAAGCCAACAGG+AGG | 0.718423 | 3.4:+93313997 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTTGCATTTAACAATT+AGG | + | chr3.4:93314438-93314457 | None:intergenic | 15.0% |
| !!! | ATAGAATGATTTTACTAATT+AGG | + | chr3.4:93314378-93314397 | None:intergenic | 15.0% |
| !!! | TTATATTTTGTGTAATTAAC+AGG | - | chr3.4:93314253-93314272 | MS.gene67054:intron | 15.0% |
| !! | CGATGAACAATAATAATATA+AGG | + | chr3.4:93314545-93314564 | None:intergenic | 20.0% |
| !!! | AGATGACATATGATTTTATA+AGG | - | chr3.4:93314058-93314077 | MS.gene67054:CDS | 20.0% |
| ! | CAAGAGAAAATATACATCTA+CGG | - | chr3.4:93314217-93314236 | MS.gene67054:intron | 25.0% |
| ! | TAATAATATAAGGCACATTG+TGG | + | chr3.4:93314535-93314554 | None:intergenic | 25.0% |
| !!! | TATTTGGTTTTGTGTTTTGT+TGG | + | chr3.4:93314005-93314024 | None:intergenic | 25.0% |
| AAACATTTCAGGGTCAATTA+TGG | - | chr3.4:93313827-93313846 | MS.gene67054:intron | 30.0% | |
| AACATTTCAGGGTCAATTAT+GGG | - | chr3.4:93313828-93313847 | MS.gene67054:intron | 30.0% | |
| AACGATTTACATGCTCATAA+CGG | + | chr3.4:93314143-93314162 | None:intergenic | 30.0% | |
| AATGTATTTGTGGTTGTTGA+AGG | - | chr3.4:93314188-93314207 | MS.gene67054:intron | 30.0% | |
| ACAAGGCAATATTTATCTGT+GGG | + | chr3.4:93313866-93313885 | None:intergenic | 30.0% | |
| TGCTTCTTGTAATGTATTTG+TGG | - | chr3.4:93314178-93314197 | MS.gene67054:intron | 30.0% | |
| ! | AACTCTGTTGATATTTTCGA+TGG | - | chr3.4:93314313-93314332 | MS.gene67054:intron | 30.0% |
| ! | AATTGACCCTGAAATGTTTT+CGG | + | chr3.4:93313826-93313845 | None:intergenic | 30.0% |
| AAGGCTTCATTGTATTCTAG+AGG | + | chr3.4:93314473-93314492 | None:intergenic | 35.0% | |
| ATACGAACCGAAAACATTTC+AGG | - | chr3.4:93313816-93313835 | MS.gene67054:intron | 35.0% | |
| ATGTTATGTGGGAATTGCAA+TGG | - | chr3.4:93314591-93314610 | MS.gene67054:CDS | 35.0% | |
| GACAAGGCAATATTTATCTG+TGG | + | chr3.4:93313867-93313886 | None:intergenic | 35.0% | |
| GTTCAAACTTCTGAATGTTC+AGG | - | chr3.4:93313770-93313789 | MS.gene67054:CDS | 35.0% | |
| TACGAACCGAAAACATTTCA+GGG | - | chr3.4:93313817-93313836 | MS.gene67054:intron | 35.0% | |
| ! | AATGAAGCCTTATGATCAGT+AGG | - | chr3.4:93314482-93314501 | MS.gene67054:CDS | 35.0% |
| ! | ATGAAGCCTTATGATCAGTA+GGG | - | chr3.4:93314483-93314502 | MS.gene67054:CDS | 35.0% |
| ! | CACTGCAAAGGTACATTTAT+GGG | - | chr3.4:93313886-93313905 | MS.gene67054:intron | 35.0% |
| ! | TCACTGCAAAGGTACATTTA+TGG | - | chr3.4:93313885-93313904 | MS.gene67054:intron | 35.0% |
| !! | ATTTTAGTGCCAAGACATGA+AGG | + | chr3.4:93313743-93313762 | None:intergenic | 35.0% |
| !!! | TTCATCGTTTTTTACTCCAG+AGG | - | chr3.4:93314558-93314577 | MS.gene67054:CDS | 35.0% |
| AATGTACCTTTGCAGTGACA+AGG | + | chr3.4:93313883-93313902 | None:intergenic | 40.0% | |
| AGGCAATATTTATCTGTGGG+AGG | + | chr3.4:93313863-93313882 | None:intergenic | 40.0% | |
| ATATTGCCTTGTCACTGCAA+AGG | - | chr3.4:93313874-93313893 | MS.gene67054:intron | 40.0% | |
| ATTGTCTGCTCTCAATGCAA+AGG | - | chr3.4:93314280-93314299 | MS.gene67054:intron | 40.0% | |
| GGCAATATTTATCTGTGGGA+GGG | + | chr3.4:93313862-93313881 | None:intergenic | 40.0% | |
| TCAGCACAGATGACACTATT+TGG | + | chr3.4:93314021-93314040 | None:intergenic | 40.0% | |
| TGCAATGGAGCTGGATTTAT+CGG | - | chr3.4:93314606-93314625 | MS.gene67054:CDS | 40.0% | |
| !! | TGTGCTGATTGTGATGGAAA+TGG | - | chr3.4:93314033-93314052 | MS.gene67054:CDS | 40.0% |
| !! | TTCGATGGACTGTTTAAAGC+TGG | - | chr3.4:93314328-93314347 | MS.gene67054:CDS | 40.0% |
| AATGGAGCTGGATTTATCGG+TGG | - | chr3.4:93314609-93314628 | MS.gene67054:CDS | 45.0% | |
| AGGGTCAATTATGGGTTCCA+AGG | - | chr3.4:93313836-93313855 | MS.gene67054:intron | 45.0% | |
| GCAGGAAGGAGATGTTATGT+GGG | - | chr3.4:93314580-93314599 | MS.gene67054:CDS | 45.0% | |
| GTTCCTCCCTACTGATCATA+AGG | + | chr3.4:93314492-93314511 | None:intergenic | 45.0% | |
| TGCCTAACACCTTCATGTCT+TGG | - | chr3.4:93313731-93313750 | MS.gene67054:CDS | 45.0% | |
| TGCTCTCAATGCAAAGGTAG+CGG | - | chr3.4:93314286-93314305 | MS.gene67054:intron | 45.0% | |
| ! | AAGCCTTATGATCAGTAGGG+AGG | - | chr3.4:93314486-93314505 | MS.gene67054:CDS | 45.0% |
| ! | TTTTTACTCCAGAGGCAGGA+AGG | - | chr3.4:93314566-93314585 | MS.gene67054:CDS | 45.0% |
| !! | GTCATCTGTGCTGATTGTGA+TGG | - | chr3.4:93314027-93314046 | MS.gene67054:CDS | 45.0% |
| !! | TGCCAAGACATGAAGGTGTT+AGG | + | chr3.4:93313736-93313755 | None:intergenic | 45.0% |
| !!! | TCGTTTTTTACTCCAGAGGC+AGG | - | chr3.4:93314562-93314581 | MS.gene67054:CDS | 45.0% |
| !! | TAATTATTTATTTATTATGT+AGG | - | chr3.4:93313966-93313985 | MS.gene67054:intron | 5.0% |
| ATTTATCTGTGGGAGGGCCT+TGG | + | chr3.4:93313856-93313875 | None:intergenic | 50.0% | |
| GGCAGGAAGGAGATGTTATG+TGG | - | chr3.4:93314579-93314598 | MS.gene67054:CDS | 50.0% | |
| TAACATCTCCTTCCTGCCTC+TGG | + | chr3.4:93314577-93314596 | None:intergenic | 50.0% | |
| TGTGGGAATTGCAATGGAGC+TGG | - | chr3.4:93314597-93314616 | MS.gene67054:CDS | 50.0% | |
| TTAAAGCTGGTGCCTCCTGT+TGG | - | chr3.4:93314341-93314360 | MS.gene67054:CDS | 50.0% | |
| TAGGTACCCGCAAAGCCAAC+AGG | + | chr3.4:93314359-93314378 | None:intergenic | 55.0% | |
| GTACCCGCAAAGCCAACAGG+AGG | + | chr3.4:93314356-93314375 | None:intergenic | 60.0% | |
| !! | GGTGCCTCCTGTTGGCTTTG+CGG | - | chr3.4:93314349-93314368 | MS.gene67054:CDS | 60.0% |
| !! | GTGCCTCCTGTTGGCTTTGC+GGG | - | chr3.4:93314350-93314369 | MS.gene67054:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 93313716 | 93314656 | 93313716 | ID=MS.gene67054 |
| chr3.4 | mRNA | 93313716 | 93314656 | 93313716 | ID=MS.gene67054.t1;Parent=MS.gene67054 |
| chr3.4 | exon | 93314477 | 93314656 | 93314477 | ID=MS.gene67054.t1.exon1;Parent=MS.gene67054.t1 |
| chr3.4 | CDS | 93314477 | 93314656 | 93314477 | ID=cds.MS.gene67054.t1;Parent=MS.gene67054.t1 |
| chr3.4 | exon | 93314318 | 93314384 | 93314318 | ID=MS.gene67054.t1.exon2;Parent=MS.gene67054.t1 |
| chr3.4 | CDS | 93314318 | 93314384 | 93314318 | ID=cds.MS.gene67054.t1;Parent=MS.gene67054.t1 |
| chr3.4 | exon | 93314001 | 93314097 | 93314001 | ID=MS.gene67054.t1.exon3;Parent=MS.gene67054.t1 |
| chr3.4 | CDS | 93314001 | 93314097 | 93314001 | ID=cds.MS.gene67054.t1;Parent=MS.gene67054.t1 |
| chr3.4 | exon | 93313716 | 93313794 | 93313716 | ID=MS.gene67054.t1.exon4;Parent=MS.gene67054.t1 |
| chr3.4 | CDS | 93313716 | 93313794 | 93313716 | ID=cds.MS.gene67054.t1;Parent=MS.gene67054.t1 |
| Gene Sequence |
| Protein sequence |
