Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70494.t1 | XP_003601472.1 | 95.3 | 149 | 3 | 1 | 1 | 149 | 31 | 175 | 7.20E-76 | 293.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70494.t1 | G7J826 | 95.3 | 149 | 3 | 1 | 1 | 149 | 31 | 175 | 5.2e-76 | 293.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049622 | MS.gene70494 | 0.8015 | 8.74E-49 | -1.69E-46 |
| MS.gene049989 | MS.gene70494 | 0.813887 | 2.04E-51 | -1.69E-46 |
| MS.gene051816 | MS.gene70494 | 0.812095 | 5.04E-51 | -1.69E-46 |
| MS.gene051914 | MS.gene70494 | 0.812337 | 4.46E-51 | -1.69E-46 |
| MS.gene051955 | MS.gene70494 | 0.846213 | 2.50E-59 | -1.69E-46 |
| MS.gene052143 | MS.gene70494 | 0.801373 | 9.28E-49 | -1.69E-46 |
| MS.gene052308 | MS.gene70494 | 0.871917 | 4.74E-67 | -1.69E-46 |
| MS.gene052367 | MS.gene70494 | 0.855705 | 5.27E-62 | -1.69E-46 |
| MS.gene053173 | MS.gene70494 | 0.85145 | 8.81E-61 | -1.69E-46 |
| MS.gene053348 | MS.gene70494 | 0.834168 | 3.50E-56 | -1.69E-46 |
| MS.gene054551 | MS.gene70494 | 0.862301 | 5.59E-64 | -1.69E-46 |
| MS.gene054552 | MS.gene70494 | 0.801893 | 7.26E-49 | -1.69E-46 |
| MS.gene054593 | MS.gene70494 | 0.844379 | 7.84E-59 | -1.69E-46 |
| MS.gene054662 | MS.gene70494 | 0.821005 | 5.08E-53 | -1.69E-46 |
| MS.gene055308 | MS.gene70494 | 0.857295 | 1.80E-62 | -1.69E-46 |
| MS.gene055578 | MS.gene70494 | 0.893177 | 8.01E-75 | -1.69E-46 |
| MS.gene055957 | MS.gene70494 | 0.862778 | 3.98E-64 | -1.69E-46 |
| MS.gene056041 | MS.gene70494 | 0.892922 | 1.02E-74 | -1.69E-46 |
| MS.gene056042 | MS.gene70494 | 0.801063 | 1.07E-48 | -1.69E-46 |
| MS.gene05640 | MS.gene70494 | 0.864625 | 1.06E-64 | -1.69E-46 |
| MS.gene056528 | MS.gene70494 | 0.821597 | 3.71E-53 | -1.69E-46 |
| MS.gene05652 | MS.gene70494 | 0.821859 | 3.23E-53 | -1.69E-46 |
| MS.gene057458 | MS.gene70494 | 0.843149 | 1.67E-58 | -1.69E-46 |
| MS.gene058013 | MS.gene70494 | 0.805196 | 1.50E-49 | -1.69E-46 |
| MS.gene058128 | MS.gene70494 | 0.844826 | 5.94E-59 | -1.69E-46 |
| MS.gene058853 | MS.gene70494 | 0.862782 | 3.97E-64 | -1.69E-46 |
| MS.gene059553 | MS.gene70494 | 0.81354 | 2.43E-51 | -1.69E-46 |
| MS.gene059616 | MS.gene70494 | 0.82804 | 1.12E-54 | -1.69E-46 |
| MS.gene060473 | MS.gene70494 | 0.860172 | 2.49E-63 | -1.69E-46 |
| MS.gene060565 | MS.gene70494 | 0.845127 | 4.93E-59 | -1.69E-46 |
| MS.gene06093 | MS.gene70494 | 0.825595 | 4.30E-54 | -1.69E-46 |
| MS.gene061099 | MS.gene70494 | 0.854216 | 1.43E-61 | -1.69E-46 |
| MS.gene061260 | MS.gene70494 | 0.868369 | 6.87E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70494.t1 | MTR_3g082070 | 95.302 | 149 | 3 | 1 | 1 | 149 | 31 | 175 | 8.97e-102 | 289 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70494.t1 | AT4G15140 | 75.000 | 40 | 9 | 1 | 1 | 39 | 32 | 71 | 8.67e-13 | 62.8 |
Find 42 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCCTTCAATCTCAAATT+TGG | 0.164475 | 3.1:+66955451 | MS.gene70494:CDS |
| ACTACTCTCAAAGCACTATT+TGG | 0.262045 | 3.1:+66955290 | MS.gene70494:CDS |
| ACCCTATTCTCCCATGGATT+TGG | 0.270878 | 3.1:+66955330 | MS.gene70494:CDS |
| AAATCATAAGCAGAGTTATC+TGG | 0.289654 | 3.1:-66955265 | None:intergenic |
| TAACCCCATCATCAATAATA+AGG | 0.309837 | 3.1:+66955552 | MS.gene70494:CDS |
| ATGTCATAGGAGAGTTTCTT+TGG | 0.315934 | 3.1:-66955364 | None:intergenic |
| ATTTGATCCTTGAAGGTGTT+GGG | 0.350471 | 3.1:-66955522 | None:intergenic |
| AAATACCTTATTATTGATGA+TGG | 0.364686 | 3.1:-66955557 | None:intergenic |
| AAGCACTATTTGGTCCCCTT+TGG | 0.370267 | 3.1:+66955300 | MS.gene70494:CDS |
| ATTTCTCCCCAACACCTTCA+AGG | 0.390688 | 3.1:+66955515 | MS.gene70494:CDS |
| TCCAAATCCATGGGAGAATA+GGG | 0.407407 | 3.1:-66955331 | None:intergenic |
| CATGCACATTTGATCCTTGA+AGG | 0.408025 | 3.1:-66955529 | None:intergenic |
| GAAACCAAATTTGAGATTGA+AGG | 0.423550 | 3.1:-66955455 | None:intergenic |
| ACAAGGTTGGTTCTAAGCTT+AGG | 0.427431 | 3.1:+66955413 | MS.gene70494:CDS |
| ATCCAAATCCATGGGAGAAT+AGG | 0.427751 | 3.1:-66955332 | None:intergenic |
| GGTAGCATCACATGAGCAAT+GGG | 0.433096 | 3.1:-66955872 | None:intergenic |
| AATACCTTATTATTGATGAT+GGG | 0.443586 | 3.1:-66955556 | None:intergenic |
| CATTTGATCCTTGAAGGTGT+TGG | 0.463929 | 3.1:-66955523 | None:intergenic |
| CTACCAATGTCTTTCAAAGA+TGG | 0.465367 | 3.1:+66955890 | MS.gene70494:CDS |
| AGCTCCTAAGCCAATGTCAT+AGG | 0.476044 | 3.1:-66955377 | None:intergenic |
| TCCATGGGAGAATAGGGTCT+TGG | 0.487632 | 3.1:-66955325 | None:intergenic |
| AATCATAAGCAGAGTTATCT+GGG | 0.497222 | 3.1:-66955264 | None:intergenic |
| AATAGGGTCTTGGAACCAAA+GGG | 0.508609 | 3.1:-66955315 | None:intergenic |
| CTCTCCTATGACATTGGCTT+AGG | 0.509647 | 3.1:+66955373 | MS.gene70494:CDS |
| AAGAAACTCTCCTATGACAT+TGG | 0.513119 | 3.1:+66955367 | MS.gene70494:CDS |
| ATACCTTATTATTGATGATG+GGG | 0.524447 | 3.1:-66955555 | None:intergenic |
| TCTCCATCTTTGAAAGACAT+TGG | 0.535368 | 3.1:-66955893 | None:intergenic |
| TGGTAGCATCACATGAGCAA+TGG | 0.550773 | 3.1:-66955873 | None:intergenic |
| AGATGCAGTGTCAACAAGGT+TGG | 0.554476 | 3.1:+66955400 | MS.gene70494:CDS |
| ATGTGATACAGAGATGGGGC+AGG | 0.556775 | 3.1:+66955798 | MS.gene70494:intron |
| TTTGATCCTTGAAGGTGTTG+GGG | 0.561529 | 3.1:-66955521 | None:intergenic |
| TCCAAGACCCTATTCTCCCA+TGG | 0.565449 | 3.1:+66955324 | MS.gene70494:CDS |
| AGTAATGAGATCCAAATCCA+TGG | 0.566074 | 3.1:-66955341 | None:intergenic |
| GAATAGGGTCTTGGAACCAA+AGG | 0.567058 | 3.1:-66955316 | None:intergenic |
| CTTCAAAACAGTTCAAGAGA+TGG | 0.567072 | 3.1:+66955834 | MS.gene70494:CDS |
| AGCTAGATGCAGTGTCAACA+AGG | 0.580501 | 3.1:+66955396 | MS.gene70494:CDS |
| AAAACAGTTCAAGAGATGGA+AGG | 0.606838 | 3.1:+66955838 | MS.gene70494:CDS |
| GTAATGAGATCCAAATCCAT+GGG | 0.641690 | 3.1:-66955340 | None:intergenic |
| TGTGATACAGAGATGGGGCA+GGG | 0.647718 | 3.1:+66955799 | MS.gene70494:intron |
| TACTATGTGATACAGAGATG+GGG | 0.699067 | 3.1:+66955794 | MS.gene70494:intron |
| ATAGGGTCTTGGAACCAAAG+GGG | 0.746078 | 3.1:-66955314 | None:intergenic |
| GTAGCATCACATGAGCAATG+GGG | 0.782689 | 3.1:-66955871 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTTTAAACTCTT+TGG | + | chr3.1:66955671-66955690 | MS.gene70494:intron | 10.0% |
| !! | AAATACCTTATTATTGATGA+TGG | - | chr3.1:66955560-66955579 | None:intergenic | 20.0% |
| !! | GATGAAATTGAAGTAATTAT+TGG | + | chr3.1:66955754-66955773 | MS.gene70494:intron | 20.0% |
| !!! | AATACCTTATTATTGATGAT+GGG | - | chr3.1:66955559-66955578 | None:intergenic | 20.0% |
| ! | AATTCCTTCAATCTCAAATT+TGG | + | chr3.1:66955451-66955470 | MS.gene70494:CDS | 25.0% |
| !! | AATTATTGGTGATAACTTGT+GGG | + | chr3.1:66955768-66955787 | MS.gene70494:intron | 25.0% |
| !! | CTGTTCTGTTTTATGATATT+GGG | + | chr3.1:66955629-66955648 | MS.gene70494:intron | 25.0% |
| !! | TAATTATTGGTGATAACTTG+TGG | + | chr3.1:66955767-66955786 | MS.gene70494:intron | 25.0% |
| !! | TCTGTTCTGTTTTATGATAT+TGG | + | chr3.1:66955628-66955647 | MS.gene70494:intron | 25.0% |
| !!! | ATACCTTATTATTGATGATG+GGG | - | chr3.1:66955558-66955577 | None:intergenic | 25.0% |
| AAATCATAAGCAGAGTTATC+TGG | - | chr3.1:66955268-66955287 | None:intergenic | 30.0% | |
| AATCATAAGCAGAGTTATCT+GGG | - | chr3.1:66955267-66955286 | None:intergenic | 30.0% | |
| TAACCCCATCATCAATAATA+AGG | + | chr3.1:66955552-66955571 | MS.gene70494:CDS | 30.0% | |
| ! | GAAACCAAATTTGAGATTGA+AGG | - | chr3.1:66955458-66955477 | None:intergenic | 30.0% |
| AAAACAGTTCAAGAGATGGA+AGG | + | chr3.1:66955838-66955857 | MS.gene70494:CDS | 35.0% | |
| AAGAAACTCTCCTATGACAT+TGG | + | chr3.1:66955367-66955386 | MS.gene70494:CDS | 35.0% | |
| AGTAATGAGATCCAAATCCA+TGG | - | chr3.1:66955344-66955363 | None:intergenic | 35.0% | |
| ATGTCATAGGAGAGTTTCTT+TGG | - | chr3.1:66955367-66955386 | None:intergenic | 35.0% | |
| CTACCAATGTCTTTCAAAGA+TGG | + | chr3.1:66955890-66955909 | MS.gene70494:CDS | 35.0% | |
| CTACTATGTGATACAGAGAT+GGG | + | chr3.1:66955793-66955812 | MS.gene70494:intron | 35.0% | |
| CTTCAAAACAGTTCAAGAGA+TGG | + | chr3.1:66955834-66955853 | MS.gene70494:CDS | 35.0% | |
| GTAATGAGATCCAAATCCAT+GGG | - | chr3.1:66955343-66955362 | None:intergenic | 35.0% | |
| TACTATGTGATACAGAGATG+GGG | + | chr3.1:66955794-66955813 | MS.gene70494:intron | 35.0% | |
| ! | ACTACTCTCAAAGCACTATT+TGG | + | chr3.1:66955290-66955309 | MS.gene70494:CDS | 35.0% |
| ! | TCTCCATCTTTGAAAGACAT+TGG | - | chr3.1:66955896-66955915 | None:intergenic | 35.0% |
| !! | ATTTGATCCTTGAAGGTGTT+GGG | - | chr3.1:66955525-66955544 | None:intergenic | 35.0% |
| AATAGGGTCTTGGAACCAAA+GGG | - | chr3.1:66955318-66955337 | None:intergenic | 40.0% | |
| ATCCAAATCCATGGGAGAAT+AGG | - | chr3.1:66955335-66955354 | None:intergenic | 40.0% | |
| CATGCACATTTGATCCTTGA+AGG | - | chr3.1:66955532-66955551 | None:intergenic | 40.0% | |
| GCTACTATGTGATACAGAGA+TGG | + | chr3.1:66955792-66955811 | MS.gene70494:intron | 40.0% | |
| TCCAAATCCATGGGAGAATA+GGG | - | chr3.1:66955334-66955353 | None:intergenic | 40.0% | |
| ! | CATTTGATCCTTGAAGGTGT+TGG | - | chr3.1:66955526-66955545 | None:intergenic | 40.0% |
| !! | ACAAGGTTGGTTCTAAGCTT+AGG | + | chr3.1:66955413-66955432 | MS.gene70494:CDS | 40.0% |
| !! | TTTGATCCTTGAAGGTGTTG+GGG | - | chr3.1:66955524-66955543 | None:intergenic | 40.0% |
| AAGCACTATTTGGTCCCCTT+TGG | + | chr3.1:66955300-66955319 | MS.gene70494:CDS | 45.0% | |
| ACCCTATTCTCCCATGGATT+TGG | + | chr3.1:66955330-66955349 | MS.gene70494:CDS | 45.0% | |
| AGATGCAGTGTCAACAAGGT+TGG | + | chr3.1:66955400-66955419 | MS.gene70494:CDS | 45.0% | |
| AGCTAGATGCAGTGTCAACA+AGG | + | chr3.1:66955396-66955415 | MS.gene70494:CDS | 45.0% | |
| AGCTCCTAAGCCAATGTCAT+AGG | - | chr3.1:66955380-66955399 | None:intergenic | 45.0% | |
| ATAGGGTCTTGGAACCAAAG+GGG | - | chr3.1:66955317-66955336 | None:intergenic | 45.0% | |
| ATTTCTCCCCAACACCTTCA+AGG | + | chr3.1:66955515-66955534 | MS.gene70494:CDS | 45.0% | |
| GAATAGGGTCTTGGAACCAA+AGG | - | chr3.1:66955319-66955338 | None:intergenic | 45.0% | |
| GGTAGCATCACATGAGCAAT+GGG | - | chr3.1:66955875-66955894 | None:intergenic | 45.0% | |
| GTAGCATCACATGAGCAATG+GGG | - | chr3.1:66955874-66955893 | None:intergenic | 45.0% | |
| TGGTAGCATCACATGAGCAA+TGG | - | chr3.1:66955876-66955895 | None:intergenic | 45.0% | |
| !! | CTCTCCTATGACATTGGCTT+AGG | + | chr3.1:66955373-66955392 | MS.gene70494:CDS | 45.0% |
| ATGTGATACAGAGATGGGGC+AGG | + | chr3.1:66955798-66955817 | MS.gene70494:intron | 50.0% | |
| TCCAAGACCCTATTCTCCCA+TGG | + | chr3.1:66955324-66955343 | MS.gene70494:CDS | 50.0% | |
| TCCATGGGAGAATAGGGTCT+TGG | - | chr3.1:66955328-66955347 | None:intergenic | 50.0% | |
| TGTGATACAGAGATGGGGCA+GGG | + | chr3.1:66955799-66955818 | MS.gene70494:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 66955238 | 66955922 | 66955238 | ID=MS.gene70494 |
| chr3.1 | mRNA | 66955238 | 66955922 | 66955238 | ID=MS.gene70494.t1;Parent=MS.gene70494 |
| chr3.1 | exon | 66955238 | 66955573 | 66955238 | ID=MS.gene70494.t1.exon1;Parent=MS.gene70494.t1 |
| chr3.1 | CDS | 66955238 | 66955573 | 66955238 | ID=cds.MS.gene70494.t1;Parent=MS.gene70494.t1 |
| chr3.1 | exon | 66955809 | 66955922 | 66955809 | ID=MS.gene70494.t1.exon2;Parent=MS.gene70494.t1 |
| chr3.1 | CDS | 66955809 | 66955922 | 66955809 | ID=cds.MS.gene70494.t1;Parent=MS.gene70494.t1 |
| Gene Sequence |
| Protein sequence |
