Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73515.t1 | XP_003605778.1 | 92.9 | 99 | 6 | 1 | 3 | 100 | 2 | 100 | 2.20E-44 | 188 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73515.t1 | B7FMD2 | 92.9 | 99 | 6 | 1 | 3 | 100 | 2 | 100 | 1.6e-44 | 188.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050060 | MS.gene73515 | 0.805946 | 1.04E-49 | -1.69E-46 |
| MS.gene050133 | MS.gene73515 | -0.817863 | 2.64E-52 | -1.69E-46 |
| MS.gene050404 | MS.gene73515 | 0.852599 | 4.15E-61 | -1.69E-46 |
| MS.gene050406 | MS.gene73515 | 0.847041 | 1.49E-59 | -1.69E-46 |
| MS.gene050426 | MS.gene73515 | -0.817457 | 3.27E-52 | -1.69E-46 |
| MS.gene051171 | MS.gene73515 | 0.864815 | 9.27E-65 | -1.69E-46 |
| MS.gene051240 | MS.gene73515 | 0.823174 | 1.60E-53 | -1.69E-46 |
| MS.gene051242 | MS.gene73515 | 0.870156 | 1.80E-66 | -1.69E-46 |
| MS.gene051244 | MS.gene73515 | 0.859667 | 3.53E-63 | -1.69E-46 |
| MS.gene052134 | MS.gene73515 | 0.837071 | 6.45E-57 | -1.69E-46 |
| MS.gene052135 | MS.gene73515 | 0.812361 | 4.41E-51 | -1.69E-46 |
| MS.gene052469 | MS.gene73515 | 0.825626 | 4.23E-54 | -1.69E-46 |
| MS.gene052589 | MS.gene73515 | 0.822955 | 1.80E-53 | -1.69E-46 |
| MS.gene052590 | MS.gene73515 | 0.808688 | 2.74E-50 | -1.69E-46 |
| MS.gene053330 | MS.gene73515 | 0.901417 | 2.74E-78 | -1.69E-46 |
| MS.gene053971 | MS.gene73515 | 0.839772 | 1.30E-57 | -1.69E-46 |
| MS.gene054102 | MS.gene73515 | 0.813862 | 2.06E-51 | -1.69E-46 |
| MS.gene054110 | MS.gene73515 | 0.818307 | 2.10E-52 | -1.69E-46 |
| MS.gene054884 | MS.gene73515 | -0.810749 | 9.88E-51 | -1.69E-46 |
| MS.gene055370 | MS.gene73515 | 0.843793 | 1.13E-58 | -1.69E-46 |
| MS.gene05553 | MS.gene73515 | 0.828705 | 7.75E-55 | -1.69E-46 |
| MS.gene056305 | MS.gene73515 | 0.836028 | 1.19E-56 | -1.69E-46 |
| MS.gene056351 | MS.gene73515 | 0.82082 | 5.60E-53 | -1.69E-46 |
| MS.gene056633 | MS.gene73515 | 0.941065 | 7.94E-101 | -1.69E-46 |
| MS.gene056664 | MS.gene73515 | 0.899108 | 2.75E-77 | -1.69E-46 |
| MS.gene057196 | MS.gene73515 | 0.82565 | 4.18E-54 | -1.69E-46 |
| MS.gene058233 | MS.gene73515 | 0.840408 | 8.85E-58 | -1.69E-46 |
| MS.gene058276 | MS.gene73515 | 0.830953 | 2.20E-55 | -1.69E-46 |
| MS.gene058743 | MS.gene73515 | 0.806348 | 8.59E-50 | -1.69E-46 |
| MS.gene058746 | MS.gene73515 | 0.931646 | 2.78E-94 | -1.69E-46 |
| MS.gene059512 | MS.gene73515 | 0.864892 | 8.77E-65 | -1.69E-46 |
| MS.gene060087 | MS.gene73515 | -0.811691 | 6.17E-51 | -1.69E-46 |
| MS.gene060407 | MS.gene73515 | 0.816217 | 6.20E-52 | -1.69E-46 |
| MS.gene061231 | MS.gene73515 | 0.817368 | 3.42E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73515.t1 | MTR_4g039540 | 92.929 | 99 | 6 | 1 | 3 | 100 | 2 | 100 | 1.88e-64 | 190 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCACTCTTGGTATAGAATT+AGG | 0.267818 | 4.4:+64514738 | MS.gene73515:CDS |
| CTTCTACCTTGGCAAGATAA+TGG | 0.344639 | 4.4:-64514773 | None:intergenic |
| GTCCACAACAAAAGGGTGAT+TGG | 0.404912 | 4.4:+64514942 | MS.gene73515:CDS |
| GTATAGAATTAGGACCCCTT+TGG | 0.412469 | 4.4:+64514748 | MS.gene73515:CDS |
| GAATTTAAGTCCACAACAAA+AGG | 0.428735 | 4.4:+64514934 | MS.gene73515:CDS |
| AAATTCTTCATCTCTTGTTG+TGG | 0.464351 | 4.4:-64514917 | None:intergenic |
| CACTCACCATTAGCCACTCT+TGG | 0.472156 | 4.4:+64514726 | MS.gene73515:CDS |
| AAGAAAGACATAGGAGGAAG+AGG | 0.481981 | 4.4:+64514827 | MS.gene73515:CDS |
| AGATGCATGGTCTTCTACCT+TGG | 0.492293 | 4.4:-64514784 | None:intergenic |
| TCTATACCAAGAGTGGCTAA+TGG | 0.502607 | 4.4:-64514732 | None:intergenic |
| GGCAAGATAATGGAACCAAA+GGG | 0.513637 | 4.4:-64514763 | None:intergenic |
| TTGAGAAGCTAATTGGGAAG+AGG | 0.524139 | 4.4:+64514894 | MS.gene73515:CDS |
| TTGGTTCCATTATCTTGCCA+AGG | 0.539176 | 4.4:+64514767 | MS.gene73515:CDS |
| CATGAAGAAAAGAAAGACAT+AGG | 0.556728 | 4.4:+64514818 | MS.gene73515:CDS |
| TGGCAAGATAATGGAACCAA+AGG | 0.571857 | 4.4:-64514764 | None:intergenic |
| TCCTAATTCTATACCAAGAG+TGG | 0.590163 | 4.4:-64514739 | None:intergenic |
| AATTTAAGTCCACAACAAAA+GGG | 0.599106 | 4.4:+64514935 | MS.gene73515:CDS |
| TGATTGGAGTTAGAGATGCA+TGG | 0.614307 | 4.4:-64514797 | None:intergenic |
| GAAGAAAAGAAAGACATAGG+AGG | 0.619275 | 4.4:+64514821 | MS.gene73515:CDS |
| GCAAGATAATGGAACCAAAG+GGG | 0.711481 | 4.4:-64514762 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTTAAGTCCACAACAAAA+GGG | + | chr4.4:64514935-64514954 | MS.gene73515:CDS | 25.0% |
| !!! | AATGATTTTGAGAAGCTAAT+TGG | + | chr4.4:64514887-64514906 | MS.gene73515:CDS | 25.0% |
| !!! | ATGATTTTGAGAAGCTAATT+GGG | + | chr4.4:64514888-64514907 | MS.gene73515:CDS | 25.0% |
| AAATTCTTCATCTCTTGTTG+TGG | - | chr4.4:64514920-64514939 | None:intergenic | 30.0% | |
| CATGAAGAAAAGAAAGACAT+AGG | + | chr4.4:64514818-64514837 | MS.gene73515:CDS | 30.0% | |
| GAATTTAAGTCCACAACAAA+AGG | + | chr4.4:64514934-64514953 | MS.gene73515:CDS | 30.0% | |
| ! | GTCTTTCTTTTCTTCATGAT+TGG | - | chr4.4:64514816-64514835 | None:intergenic | 30.0% |
| GAAGAAAAGAAAGACATAGG+AGG | + | chr4.4:64514821-64514840 | MS.gene73515:CDS | 35.0% | |
| TCCTAATTCTATACCAAGAG+TGG | - | chr4.4:64514742-64514761 | None:intergenic | 35.0% | |
| AAGAAAGACATAGGAGGAAG+AGG | + | chr4.4:64514827-64514846 | MS.gene73515:CDS | 40.0% | |
| CTTCTACCTTGGCAAGATAA+TGG | - | chr4.4:64514776-64514795 | None:intergenic | 40.0% | |
| GCCACTCTTGGTATAGAATT+AGG | + | chr4.4:64514738-64514757 | MS.gene73515:CDS | 40.0% | |
| GTATAGAATTAGGACCCCTT+TGG | + | chr4.4:64514748-64514767 | MS.gene73515:CDS | 40.0% | |
| TCTATACCAAGAGTGGCTAA+TGG | - | chr4.4:64514735-64514754 | None:intergenic | 40.0% | |
| TGATTGGAGTTAGAGATGCA+TGG | - | chr4.4:64514800-64514819 | None:intergenic | 40.0% | |
| TTGAGAAGCTAATTGGGAAG+AGG | + | chr4.4:64514894-64514913 | MS.gene73515:CDS | 40.0% | |
| TTGGTTCCATTATCTTGCCA+AGG | + | chr4.4:64514767-64514786 | MS.gene73515:CDS | 40.0% | |
| ! | TTCCAATCACCCTTTTGTTG+TGG | - | chr4.4:64514947-64514966 | None:intergenic | 40.0% |
| !! | GCAAGATAATGGAACCAAAG+GGG | - | chr4.4:64514765-64514784 | None:intergenic | 40.0% |
| !! | GGCAAGATAATGGAACCAAA+GGG | - | chr4.4:64514766-64514785 | None:intergenic | 40.0% |
| !! | TGGCAAGATAATGGAACCAA+AGG | - | chr4.4:64514767-64514786 | None:intergenic | 40.0% |
| AGATGCATGGTCTTCTACCT+TGG | - | chr4.4:64514787-64514806 | None:intergenic | 45.0% | |
| GTCCACAACAAAAGGGTGAT+TGG | + | chr4.4:64514942-64514961 | MS.gene73515:CDS | 45.0% | |
| CACTCACCATTAGCCACTCT+TGG | + | chr4.4:64514726-64514745 | MS.gene73515:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 64514719 | 64515020 | 64514719 | ID=MS.gene73515 |
| chr4.4 | mRNA | 64514719 | 64515020 | 64514719 | ID=MS.gene73515.t1;Parent=MS.gene73515 |
| chr4.4 | exon | 64514719 | 64515020 | 64514719 | ID=MS.gene73515.t1.exon1;Parent=MS.gene73515.t1 |
| chr4.4 | CDS | 64514719 | 64515020 | 64514719 | ID=cds.MS.gene73515.t1;Parent=MS.gene73515.t1 |
| Gene Sequence |
| Protein sequence |
