AlfalfaGEDB | MS.gene73823

M. sativa cultivar XinJiangDaYe / MS.gene73823

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73823.t1	XP_003607563.1	100	77	0	0	1	77	1	77	4.90E-20	106.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73823.t1	O82275	64.9	77	25	1	1	77	1	75	1.4e-15	83.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73823.t1	G7JV20	100.0	77	0	0	1	77	1	77	3.6e-20	106.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049499	MS.gene73823	0.801696	7.97E-49	-1.69E-46
MS.gene049680	MS.gene73823	0.849815	2.54E-60	-1.69E-46
MS.gene050752	MS.gene73823	0.801418	9.09E-49	-1.69E-46
MS.gene050945	MS.gene73823	0.822795	1.96E-53	-1.69E-46
MS.gene050955	MS.gene73823	0.821716	3.48E-53	-1.69E-46
MS.gene051699	MS.gene73823	0.855827	4.86E-62	-1.69E-46
MS.gene052533	MS.gene73823	0.81902	1.45E-52	-1.69E-46
MS.gene052793	MS.gene73823	0.833263	5.90E-56	-1.69E-46
MS.gene053272	MS.gene73823	-0.806408	8.35E-50	-1.69E-46
MS.gene053963	MS.gene73823	0.80347	3.43E-49	-1.69E-46
MS.gene053995	MS.gene73823	0.801868	7.35E-49	-1.69E-46
MS.gene054222	MS.gene73823	0.822871	1.88E-53	-1.69E-46
MS.gene054403	MS.gene73823	0.809345	1.98E-50	-1.69E-46
MS.gene054490	MS.gene73823	0.801211	1.00E-48	-1.69E-46
MS.gene05548	MS.gene73823	0.806012	1.01E-49	-1.69E-46
MS.gene055555	MS.gene73823	0.85138	9.22E-61	-1.69E-46
MS.gene056677	MS.gene73823	0.81898	1.48E-52	-1.69E-46
MS.gene057102	MS.gene73823	0.859819	3.18E-63	-1.69E-46
MS.gene057208	MS.gene73823	0.802994	4.31E-49	-1.69E-46
MS.gene057694	MS.gene73823	0.804972	1.67E-49	-1.69E-46
MS.gene057705	MS.gene73823	0.84261	2.33E-58	-1.69E-46
MS.gene058008	MS.gene73823	0.815344	9.70E-52	-1.69E-46
MS.gene058159	MS.gene73823	0.804916	1.72E-49	-1.69E-46
MS.gene05952	MS.gene73823	0.817067	4.00E-52	-1.69E-46
MS.gene05985	MS.gene73823	-0.857268	1.83E-62	-1.69E-46
MS.gene059901	MS.gene73823	-0.812807	3.52E-51	-1.69E-46
MS.gene059935	MS.gene73823	0.804998	1.65E-49	-1.69E-46
MS.gene060365	MS.gene73823	0.804313	2.29E-49	-1.69E-46
MS.gene061100	MS.gene73823	0.807036	6.15E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73823.t1	MTR_4g079680	100.000	77	0	0	1	77	1	77	2.61e-48	147
MS.gene73823.t1	MTR_2g015540	75.000	76	19	0	2	77	5	80	5.78e-35	114
MS.gene73823.t1	MTR_2g090695	58.974	78	31	1	1	77	1	78	8.32e-21	78.2
MS.gene73823.t1	MTR_4g078840	53.521	71	33	0	7	77	9	79	3.55e-14	61.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene73823.t1	AT2G31090	76.712	73	17	0	5	77	3	75	4.61e-29	99.0
MS.gene73823.t1	AT2G20562	52.778	72	34	0	6	77	2	73	2.85e-21	79.3

Find 17 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAATCGTTGGTGTACTCATC+TGG	0.378938	4.4:-31482358	MS.gene73823:CDS
CTCTCAGCAGGGGATCTTAG+AGG	0.383799	4.4:+31482117	None:intergenic
GGTGTACTCATCTGGATTGT+CGG	0.396564	4.4:-31482350	MS.gene73823:CDS
GAAGGTGAATGCAGACCATT+GGG	0.445910	4.4:-31482425	MS.gene73823:CDS
TGTGGAACTTGCGCTTGAAT+TGG	0.449919	4.4:-31482173	MS.gene73823:CDS
AGAAGGTGAATGCAGACCAT+TGG	0.487199	4.4:-31482426	MS.gene73823:CDS
GTGTACTCATCTGGATTGTC+GGG	0.488369	4.4:-31482349	MS.gene73823:intron
TTTATGCTTAACTATAATTG+TGG	0.504363	4.4:-31482191	MS.gene73823:CDS
TGTCTTCTCATCTCAATCGT+TGG	0.507454	4.4:-31482371	MS.gene73823:CDS
CAAAGCTCCATTGCATGTCA+TGG	0.535851	4.4:-31482149	MS.gene73823:CDS
AACCACTCCATGACATGCAA+TGG	0.552057	4.4:+31482142	None:intergenic
GAAAAGATGTGCAATTCAGA+AGG	0.574414	4.4:-31482443	None:intergenic
AGAAGACAGAGAAAAGCGAA+AGG	0.608508	4.4:+31482386	None:intergenic
CTCCATTGCATGTCATGGAG+TGG	0.615369	4.4:-31482144	MS.gene73823:CDS
ATAGTTAAGCATAAACAACA+AGG	0.675018	4.4:+31482199	None:intergenic
AGACCTAATAGAAAACCCAA+TGG	0.675649	4.4:+31482410	None:intergenic
TAGTTAAGCATAAACAACAA+GGG	0.709299	4.4:+31482200	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTATGCTTAACTATAATTG+TGG	-	chr4.4:31482366-31482385	MS.gene73823:CDS	20.0%
!!!	TTTATTCATATTTTGCAGAT+TGG	-	chr4.4:31482318-31482337	MS.gene73823:intron	20.0%
!	ATAGTTAAGCATAAACAACA+AGG	+	chr4.4:31482361-31482380	None:intergenic	25.0%
!	GATTTCTTCATATTTGATCT+GGG	-	chr4.4:31482264-31482283	MS.gene73823:intron	25.0%
!	TAGTTAAGCATAAACAACAA+GGG	+	chr4.4:31482360-31482379	None:intergenic	25.0%
!	TGATTTCTTCATATTTGATC+TGG	-	chr4.4:31482263-31482282	MS.gene73823:intron	25.0%
	AGACCTAATAGAAAACCCAA+TGG	+	chr4.4:31482150-31482169	None:intergenic	35.0%
!	AGACCATTGGGTTTTCTATT+AGG	-	chr4.4:31482144-31482163	MS.gene73823:CDS	35.0%
	AATTACTCTTCCCACTCTTC+TGG	-	chr4.4:31482233-31482252	MS.gene73823:intron	40.0%
	AGAAGACAGAGAAAAGCGAA+AGG	+	chr4.4:31482174-31482193	None:intergenic	40.0%
	ATCAAAGTAACCCAGAAGAG+TGG	+	chr4.4:31482247-31482266	None:intergenic	40.0%
	ATTACTCTTCCCACTCTTCT+GGG	-	chr4.4:31482234-31482253	MS.gene73823:intron	40.0%
	TCAAAGTAACCCAGAAGAGT+GGG	+	chr4.4:31482246-31482265	None:intergenic	40.0%
	TGTCTTCTCATCTCAATCGT+TGG	-	chr4.4:31482186-31482205	MS.gene73823:CDS	40.0%
	AACCACTCCATGACATGCAA+TGG	+	chr4.4:31482418-31482437	None:intergenic	45.0%
	AGAAGGTGAATGCAGACCAT+TGG	-	chr4.4:31482131-31482150	MS.gene73823:CDS	45.0%
	CAAAGCTCCATTGCATGTCA+TGG	-	chr4.4:31482408-31482427	MS.gene73823:CDS	45.0%
	CAATCGTTGGTGTACTCATC+TGG	-	chr4.4:31482199-31482218	MS.gene73823:CDS	45.0%
	GAAGGTGAATGCAGACCATT+GGG	-	chr4.4:31482132-31482151	MS.gene73823:CDS	45.0%
	GGTGTACTCATCTGGATTGT+CGG	-	chr4.4:31482207-31482226	MS.gene73823:CDS	45.0%
	GTGTACTCATCTGGATTGTC+GGG	-	chr4.4:31482208-31482227	MS.gene73823:CDS	45.0%
	TGTGGAACTTGCGCTTGAAT+TGG	-	chr4.4:31482384-31482403	MS.gene73823:CDS	45.0%
	CTCCATTGCATGTCATGGAG+TGG	-	chr4.4:31482413-31482432	MS.gene73823:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	31482120	31482459	31482120	ID=MS.gene73823
chr4.4	mRNA	31482120	31482459	31482120	ID=MS.gene73823.t1;Parent=MS.gene73823
chr4.4	exon	31482350	31482459	31482350	ID=MS.gene73823.t1.exon1;Parent=MS.gene73823.t1
chr4.4	CDS	31482350	31482459	31482350	ID=cds.MS.gene73823.t1;Parent=MS.gene73823.t1
chr4.4	exon	31482120	31482243	31482120	ID=MS.gene73823.t1.exon2;Parent=MS.gene73823.t1
chr4.4	CDS	31482120	31482243	31482120	ID=cds.MS.gene73823.t1;Parent=MS.gene73823.t1

Gene Sequence	>MS.gene73823 ATGTGCAATTCAGAAGGTGAATGCAGACCATTGGGTTTTCTATTAGGTCTTCCTTTCGCTTTTCTCTGTCTTCTCATCTCAATCGTTGGTGTACTCATCTGGATTGTCGGGTAAATTACTCTTCCCACTCTTCTGGGTTACTTTGATTTCTTCATATTTGATCTGGGTTTCGATCTAATCGCTATAAAAATCAAAACTTTTATTCATATTTTGCAGATTGGCGCTTACTTGTATATGCCCTTGTTGTTTATGCTTAACTATAATTGTGGAACTTGCGCTTGAATTGGTCAAAGCTCCATTGCATGTCATGGAGTGGTTCACCTCTAAGATCCCCTGCTGA
Protein sequence	>MS.gene73823.t1 MCNSEGECRPLGFLLGLPFAFLCLLISIVGVLIWIVGLALTCICPCCLCLTIIVELALELVKAPLHVMEWFTSKIPC