Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73897.t1 | XP_003601472.1 | 95.3 | 149 | 3 | 1 | 1 | 149 | 31 | 175 | 7.20E-76 | 293.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73897.t1 | G7J826 | 95.3 | 149 | 3 | 1 | 1 | 149 | 31 | 175 | 5.2e-76 | 293.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049622 | MS.gene73897 | 0.820964 | 5.19E-53 | -1.69E-46 |
| MS.gene049989 | MS.gene73897 | 0.808635 | 2.81E-50 | -1.69E-46 |
| MS.gene051816 | MS.gene73897 | 0.827978 | 1.16E-54 | -1.69E-46 |
| MS.gene051955 | MS.gene73897 | 0.847274 | 1.28E-59 | -1.69E-46 |
| MS.gene052143 | MS.gene73897 | 0.815379 | 9.53E-52 | -1.69E-46 |
| MS.gene052308 | MS.gene73897 | 0.868391 | 6.76E-66 | -1.69E-46 |
| MS.gene052367 | MS.gene73897 | 0.851417 | 8.99E-61 | -1.69E-46 |
| MS.gene053173 | MS.gene73897 | 0.829824 | 4.15E-55 | -1.69E-46 |
| MS.gene053348 | MS.gene73897 | 0.830681 | 2.56E-55 | -1.69E-46 |
| MS.gene053416 | MS.gene73897 | 0.801914 | 7.19E-49 | -1.69E-46 |
| MS.gene053417 | MS.gene73897 | 0.803611 | 3.21E-49 | -1.69E-46 |
| MS.gene054551 | MS.gene73897 | 0.846688 | 1.86E-59 | -1.69E-46 |
| MS.gene054552 | MS.gene73897 | 0.801852 | 7.40E-49 | -1.69E-46 |
| MS.gene054593 | MS.gene73897 | 0.836319 | 1.00E-56 | -1.69E-46 |
| MS.gene054662 | MS.gene73897 | 0.802724 | 4.90E-49 | -1.69E-46 |
| MS.gene055308 | MS.gene73897 | 0.844206 | 8.73E-59 | -1.69E-46 |
| MS.gene055578 | MS.gene73897 | 0.873115 | 1.89E-67 | -1.69E-46 |
| MS.gene055579 | MS.gene73897 | 0.802855 | 4.60E-49 | -1.69E-46 |
| MS.gene055957 | MS.gene73897 | 0.862515 | 4.80E-64 | -1.69E-46 |
| MS.gene056041 | MS.gene73897 | 0.887517 | 1.33E-72 | -1.69E-46 |
| MS.gene056042 | MS.gene73897 | 0.813743 | 2.19E-51 | -1.69E-46 |
| MS.gene05640 | MS.gene73897 | 0.841576 | 4.37E-58 | -1.69E-46 |
| MS.gene056526 | MS.gene73897 | 0.821364 | 4.20E-53 | -1.69E-46 |
| MS.gene056528 | MS.gene73897 | 0.817111 | 3.91E-52 | -1.69E-46 |
| MS.gene057458 | MS.gene73897 | 0.837028 | 6.62E-57 | -1.69E-46 |
| MS.gene058128 | MS.gene73897 | 0.828407 | 9.15E-55 | -1.69E-46 |
| MS.gene058849 | MS.gene73897 | 0.809284 | 2.04E-50 | -1.69E-46 |
| MS.gene058853 | MS.gene73897 | 0.850198 | 1.98E-60 | -1.69E-46 |
| MS.gene059553 | MS.gene73897 | 0.814119 | 1.81E-51 | -1.69E-46 |
| MS.gene059616 | MS.gene73897 | 0.821324 | 4.29E-53 | -1.69E-46 |
| MS.gene060473 | MS.gene73897 | 0.862122 | 6.34E-64 | -1.69E-46 |
| MS.gene060565 | MS.gene73897 | 0.84633 | 2.32E-59 | -1.69E-46 |
| MS.gene06093 | MS.gene73897 | 0.814771 | 1.30E-51 | -1.69E-46 |
| MS.gene061099 | MS.gene73897 | 0.857355 | 1.73E-62 | -1.69E-46 |
| MS.gene061260 | MS.gene73897 | 0.872366 | 3.36E-67 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73897.t1 | MTR_3g082070 | 95.302 | 149 | 3 | 1 | 1 | 149 | 31 | 175 | 8.97e-102 | 289 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73897.t1 | AT4G15140 | 75.000 | 40 | 9 | 1 | 1 | 39 | 32 | 71 | 8.67e-13 | 62.8 |
Find 42 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATTCCTTCAATCTCAAATT+TGG | 0.164475 | 3.2:-71486478 | MS.gene73897:CDS |
| ACTACTCTCAAAGCACTATT+TGG | 0.262045 | 3.2:-71486639 | MS.gene73897:CDS |
| ACCCTATTCTCCCATGGATT+TGG | 0.270878 | 3.2:-71486599 | MS.gene73897:CDS |
| AAATCATAAGCAGAGTTATC+TGG | 0.289654 | 3.2:+71486664 | None:intergenic |
| TAACCCCATCATCAATAATA+AGG | 0.309837 | 3.2:-71486377 | MS.gene73897:intron |
| ATGTCATAGGAGAGTTTCTT+TGG | 0.315934 | 3.2:+71486565 | None:intergenic |
| ATTTGATCCTTGAAGGTGTT+GGG | 0.350471 | 3.2:+71486407 | None:intergenic |
| AAATACCTTATTATTGATGA+TGG | 0.364686 | 3.2:+71486372 | None:intergenic |
| AAGCACTATTTGGTCCCCTT+TGG | 0.370267 | 3.2:-71486629 | MS.gene73897:CDS |
| ATTTCTCCCCAACACCTTCA+AGG | 0.390688 | 3.2:-71486414 | MS.gene73897:CDS |
| TCCAAATCCATGGGAGAATA+GGG | 0.407407 | 3.2:+71486598 | None:intergenic |
| CATGCACATTTGATCCTTGA+AGG | 0.408025 | 3.2:+71486400 | None:intergenic |
| GAAACCAAATTTGAGATTGA+AGG | 0.423550 | 3.2:+71486474 | None:intergenic |
| ACAAGGTTGGTTCTAAGCTT+AGG | 0.427431 | 3.2:-71486516 | MS.gene73897:CDS |
| ATCCAAATCCATGGGAGAAT+AGG | 0.427751 | 3.2:+71486597 | None:intergenic |
| GGTAGCATCACATGAGCAAT+GGG | 0.433096 | 3.2:+71486048 | None:intergenic |
| AATACCTTATTATTGATGAT+GGG | 0.443586 | 3.2:+71486373 | None:intergenic |
| CATTTGATCCTTGAAGGTGT+TGG | 0.463929 | 3.2:+71486406 | None:intergenic |
| CTACCAATGTCTTTCAAAGA+TGG | 0.465367 | 3.2:-71486030 | MS.gene73897:CDS |
| AGCTCCTAAGCCAATGTCAT+AGG | 0.476044 | 3.2:+71486552 | None:intergenic |
| TCCATGGGAGAATAGGGTCT+TGG | 0.487632 | 3.2:+71486604 | None:intergenic |
| AATCATAAGCAGAGTTATCT+GGG | 0.497222 | 3.2:+71486665 | None:intergenic |
| AATAGGGTCTTGGAACCAAA+GGG | 0.508609 | 3.2:+71486614 | None:intergenic |
| CTCTCCTATGACATTGGCTT+AGG | 0.509647 | 3.2:-71486556 | MS.gene73897:CDS |
| AAGAAACTCTCCTATGACAT+TGG | 0.513119 | 3.2:-71486562 | MS.gene73897:CDS |
| ATACCTTATTATTGATGATG+GGG | 0.524447 | 3.2:+71486374 | None:intergenic |
| TCTCCATCTTTGAAAGACAT+TGG | 0.535368 | 3.2:+71486027 | None:intergenic |
| TGGTAGCATCACATGAGCAA+TGG | 0.550773 | 3.2:+71486047 | None:intergenic |
| AGATGCAGTGTCAACAAGGT+TGG | 0.554476 | 3.2:-71486529 | MS.gene73897:CDS |
| ATGTGATACAGAGATGGGGC+AGG | 0.556775 | 3.2:-71486122 | MS.gene73897:intron |
| TTTGATCCTTGAAGGTGTTG+GGG | 0.561529 | 3.2:+71486408 | None:intergenic |
| TCCAAGACCCTATTCTCCCA+TGG | 0.565449 | 3.2:-71486605 | MS.gene73897:CDS |
| AGTAATGAGATCCAAATCCA+TGG | 0.566074 | 3.2:+71486588 | None:intergenic |
| GAATAGGGTCTTGGAACCAA+AGG | 0.567058 | 3.2:+71486613 | None:intergenic |
| CTTCAAAACAGTTCAAGAGA+TGG | 0.567072 | 3.2:-71486086 | MS.gene73897:CDS |
| AGCTAGATGCAGTGTCAACA+AGG | 0.580501 | 3.2:-71486533 | MS.gene73897:CDS |
| AAAACAGTTCAAGAGATGGA+AGG | 0.606838 | 3.2:-71486082 | MS.gene73897:CDS |
| GTAATGAGATCCAAATCCAT+GGG | 0.641690 | 3.2:+71486589 | None:intergenic |
| TGTGATACAGAGATGGGGCA+GGG | 0.647718 | 3.2:-71486121 | MS.gene73897:intron |
| TACTATGTGATACAGAGATG+GGG | 0.699067 | 3.2:-71486126 | MS.gene73897:intron |
| ATAGGGTCTTGGAACCAAAG+GGG | 0.746078 | 3.2:+71486615 | None:intergenic |
| GTAGCATCACATGAGCAATG+GGG | 0.782689 | 3.2:+71486049 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTTTTTTAAACTCTT+TGG | - | chr3.2:71486462-71486481 | MS.gene73897:CDS | 10.0% |
| !! | AAATACCTTATTATTGATGA+TGG | + | chr3.2:71486342-71486361 | None:intergenic | 20.0% |
| !! | GATGAAATTGAAGTAATTAT+TGG | - | chr3.2:71486545-71486564 | MS.gene73897:CDS | 20.0% |
| !!! | AATACCTTATTATTGATGAT+GGG | + | chr3.2:71486341-71486360 | None:intergenic | 20.0% |
| ! | AATTCCTTCAATCTCAAATT+TGG | - | chr3.2:71486233-71486252 | MS.gene73897:intron | 25.0% |
| !! | AATTATTGGTGATAACTTGT+GGG | - | chr3.2:71486559-71486578 | MS.gene73897:CDS | 25.0% |
| !! | CTGTTCTGTTTTATGATATT+GGG | - | chr3.2:71486411-71486430 | MS.gene73897:CDS | 25.0% |
| !! | TAATTATTGGTGATAACTTG+TGG | - | chr3.2:71486558-71486577 | MS.gene73897:CDS | 25.0% |
| !! | TCTGTTCTGTTTTATGATAT+TGG | - | chr3.2:71486410-71486429 | MS.gene73897:CDS | 25.0% |
| !!! | ATACCTTATTATTGATGATG+GGG | + | chr3.2:71486340-71486359 | None:intergenic | 25.0% |
| AAATCATAAGCAGAGTTATC+TGG | + | chr3.2:71486050-71486069 | None:intergenic | 30.0% | |
| AATCATAAGCAGAGTTATCT+GGG | + | chr3.2:71486049-71486068 | None:intergenic | 30.0% | |
| TAACCCCATCATCAATAATA+AGG | - | chr3.2:71486334-71486353 | MS.gene73897:intron | 30.0% | |
| ! | GAAACCAAATTTGAGATTGA+AGG | + | chr3.2:71486240-71486259 | None:intergenic | 30.0% |
| AAAACAGTTCAAGAGATGGA+AGG | - | chr3.2:71486629-71486648 | MS.gene73897:CDS | 35.0% | |
| AAGAAACTCTCCTATGACAT+TGG | - | chr3.2:71486149-71486168 | MS.gene73897:intron | 35.0% | |
| AGTAATGAGATCCAAATCCA+TGG | + | chr3.2:71486126-71486145 | None:intergenic | 35.0% | |
| ATGTCATAGGAGAGTTTCTT+TGG | + | chr3.2:71486149-71486168 | None:intergenic | 35.0% | |
| CTACCAATGTCTTTCAAAGA+TGG | - | chr3.2:71486681-71486700 | MS.gene73897:CDS | 35.0% | |
| CTACTATGTGATACAGAGAT+GGG | - | chr3.2:71486584-71486603 | MS.gene73897:CDS | 35.0% | |
| CTTCAAAACAGTTCAAGAGA+TGG | - | chr3.2:71486625-71486644 | MS.gene73897:CDS | 35.0% | |
| GTAATGAGATCCAAATCCAT+GGG | + | chr3.2:71486125-71486144 | None:intergenic | 35.0% | |
| TACTATGTGATACAGAGATG+GGG | - | chr3.2:71486585-71486604 | MS.gene73897:CDS | 35.0% | |
| ! | ACTACTCTCAAAGCACTATT+TGG | - | chr3.2:71486072-71486091 | MS.gene73897:CDS | 35.0% |
| ! | TCTCCATCTTTGAAAGACAT+TGG | + | chr3.2:71486687-71486706 | None:intergenic | 35.0% |
| !! | ATTTGATCCTTGAAGGTGTT+GGG | + | chr3.2:71486307-71486326 | None:intergenic | 35.0% |
| AATAGGGTCTTGGAACCAAA+GGG | + | chr3.2:71486100-71486119 | None:intergenic | 40.0% | |
| ATCCAAATCCATGGGAGAAT+AGG | + | chr3.2:71486117-71486136 | None:intergenic | 40.0% | |
| CATGCACATTTGATCCTTGA+AGG | + | chr3.2:71486314-71486333 | None:intergenic | 40.0% | |
| GCTACTATGTGATACAGAGA+TGG | - | chr3.2:71486583-71486602 | MS.gene73897:CDS | 40.0% | |
| TCCAAATCCATGGGAGAATA+GGG | + | chr3.2:71486116-71486135 | None:intergenic | 40.0% | |
| ! | CATTTGATCCTTGAAGGTGT+TGG | + | chr3.2:71486308-71486327 | None:intergenic | 40.0% |
| !! | ACAAGGTTGGTTCTAAGCTT+AGG | - | chr3.2:71486195-71486214 | MS.gene73897:intron | 40.0% |
| !! | TTTGATCCTTGAAGGTGTTG+GGG | + | chr3.2:71486306-71486325 | None:intergenic | 40.0% |
| AAGCACTATTTGGTCCCCTT+TGG | - | chr3.2:71486082-71486101 | MS.gene73897:CDS | 45.0% | |
| ACCCTATTCTCCCATGGATT+TGG | - | chr3.2:71486112-71486131 | MS.gene73897:CDS | 45.0% | |
| AGATGCAGTGTCAACAAGGT+TGG | - | chr3.2:71486182-71486201 | MS.gene73897:intron | 45.0% | |
| AGCTAGATGCAGTGTCAACA+AGG | - | chr3.2:71486178-71486197 | MS.gene73897:intron | 45.0% | |
| AGCTCCTAAGCCAATGTCAT+AGG | + | chr3.2:71486162-71486181 | None:intergenic | 45.0% | |
| ATAGGGTCTTGGAACCAAAG+GGG | + | chr3.2:71486099-71486118 | None:intergenic | 45.0% | |
| ATTTCTCCCCAACACCTTCA+AGG | - | chr3.2:71486297-71486316 | MS.gene73897:intron | 45.0% | |
| GAATAGGGTCTTGGAACCAA+AGG | + | chr3.2:71486101-71486120 | None:intergenic | 45.0% | |
| GGTAGCATCACATGAGCAAT+GGG | + | chr3.2:71486666-71486685 | None:intergenic | 45.0% | |
| GTAGCATCACATGAGCAATG+GGG | + | chr3.2:71486665-71486684 | None:intergenic | 45.0% | |
| TGGTAGCATCACATGAGCAA+TGG | + | chr3.2:71486667-71486686 | None:intergenic | 45.0% | |
| !! | CTCTCCTATGACATTGGCTT+AGG | - | chr3.2:71486155-71486174 | MS.gene73897:intron | 45.0% |
| ATGTGATACAGAGATGGGGC+AGG | - | chr3.2:71486589-71486608 | MS.gene73897:CDS | 50.0% | |
| TCCAAGACCCTATTCTCCCA+TGG | - | chr3.2:71486106-71486125 | MS.gene73897:CDS | 50.0% | |
| TCCATGGGAGAATAGGGTCT+TGG | + | chr3.2:71486110-71486129 | None:intergenic | 50.0% | |
| TGTGATACAGAGATGGGGCA+GGG | - | chr3.2:71486590-71486609 | MS.gene73897:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 71486020 | 71486713 | 71486020 | ID=MS.gene73897 |
| chr3.2 | mRNA | 71486020 | 71486713 | 71486020 | ID=MS.gene73897.t1;Parent=MS.gene73897 |
| chr3.2 | exon | 71486378 | 71486713 | 71486378 | ID=MS.gene73897.t1.exon1;Parent=MS.gene73897.t1 |
| chr3.2 | CDS | 71486378 | 71486713 | 71486378 | ID=cds.MS.gene73897.t1;Parent=MS.gene73897.t1 |
| chr3.2 | exon | 71486020 | 71486133 | 71486020 | ID=MS.gene73897.t1.exon2;Parent=MS.gene73897.t1 |
| chr3.2 | CDS | 71486020 | 71486133 | 71486020 | ID=cds.MS.gene73897.t1;Parent=MS.gene73897.t1 |
| Gene Sequence |
| Protein sequence |
