Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74500.t1 | XP_003611853.1 | 95.7 | 161 | 7 | 0 | 1 | 161 | 1 | 161 | 1.90E-50 | 208.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74500.t1 | G7K9G8 | 95.7 | 161 | 7 | 0 | 1 | 161 | 1 | 161 | 1.4e-50 | 208.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene74500 | 0.809591 | 1.75E-50 | -1.69E-46 |
| MS.gene049491 | MS.gene74500 | 0.811329 | 7.39E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene74500 | 0.801142 | 1.04E-48 | -1.69E-46 |
| MS.gene049910 | MS.gene74500 | 0.837854 | 4.07E-57 | -1.69E-46 |
| MS.gene050301 | MS.gene74500 | 0.814663 | 1.37E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene74500 | 0.809442 | 1.89E-50 | -1.69E-46 |
| MS.gene051860 | MS.gene74500 | 0.807385 | 5.19E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene74500 | 0.808296 | 3.32E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene74500 | 0.804205 | 2.42E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene74500 | 0.836594 | 8.54E-57 | -1.69E-46 |
| MS.gene053836 | MS.gene74500 | 0.849313 | 3.50E-60 | -1.69E-46 |
| MS.gene054349 | MS.gene74500 | 0.804551 | 2.05E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene74500 | 0.826037 | 3.38E-54 | -1.69E-46 |
| MS.gene054983 | MS.gene74500 | 0.806164 | 9.39E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene74500 | 0.817311 | 3.52E-52 | -1.69E-46 |
| MS.gene055422 | MS.gene74500 | 0.805895 | 1.07E-49 | -1.69E-46 |
| MS.gene055428 | MS.gene74500 | 0.803451 | 3.46E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene74500 | 0.813215 | 2.86E-51 | -1.69E-46 |
| MS.gene056191 | MS.gene74500 | 0.803752 | 3.00E-49 | -1.69E-46 |
| MS.gene056723 | MS.gene74500 | 0.811838 | 5.73E-51 | -1.69E-46 |
| MS.gene056875 | MS.gene74500 | 0.814355 | 1.61E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene74500 | 0.83245 | 9.39E-56 | -1.69E-46 |
| MS.gene057749 | MS.gene74500 | 0.804211 | 2.41E-49 | -1.69E-46 |
| MS.gene057750 | MS.gene74500 | 0.811221 | 7.80E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene74500 | 0.852351 | 4.89E-61 | -1.69E-46 |
| MS.gene05811 | MS.gene74500 | 0.807406 | 5.13E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene74500 | 0.833043 | 6.69E-56 | -1.69E-46 |
| MS.gene059285 | MS.gene74500 | 0.819742 | 9.90E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene74500 | 0.855538 | 5.90E-62 | -1.69E-46 |
| MS.gene059688 | MS.gene74500 | 0.80236 | 5.82E-49 | -1.69E-46 |
| MS.gene059692 | MS.gene74500 | 0.826914 | 2.09E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene74500 | 0.827158 | 1.83E-54 | -1.69E-46 |
| MS.gene05984 | MS.gene74500 | 0.817979 | 2.49E-52 | -1.69E-46 |
| MS.gene059894 | MS.gene74500 | 0.818026 | 2.43E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene74500 | 0.849661 | 2.80E-60 | -1.69E-46 |
| MS.gene061051 | MS.gene74500 | -0.802074 | 6.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74500.t1 | MTR_5g018590 | 95.652 | 161 | 7 | 0 | 1 | 161 | 1 | 161 | 2.52e-105 | 298 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74500.t1 | AT2G23670 | 64.706 | 119 | 42 | 0 | 43 | 161 | 49 | 167 | 2.92e-44 | 144 |
Find 51 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCGGTGGTGAATGGTATTT+TGG | 0.145285 | 5.2:-10415819 | MS.gene74500:CDS |
| GCGCAGGATATTCCTCTGTT+TGG | 0.296318 | 5.2:-10416067 | MS.gene74500:CDS |
| GGATTGGAGACGGCGGAAAA+AGG | 0.331002 | 5.2:-10415968 | MS.gene74500:CDS |
| GGCAAGGGAATCGGTGTCTA+TGG | 0.348419 | 5.2:-10416089 | MS.gene74500:CDS |
| CGCAGGATATTCCTCTGTTT+GGG | 0.350852 | 5.2:-10416066 | MS.gene74500:CDS |
| GACGGCGTCTATTGCTTTAA+CGG | 0.381963 | 5.2:-10416110 | MS.gene74500:CDS |
| TGCTTTAACGGCAAGGGAAT+CGG | 0.392964 | 5.2:-10416098 | MS.gene74500:CDS |
| GTTGCTTCGGCGGTGGTGAA+TGG | 0.416909 | 5.2:-10415827 | MS.gene74500:CDS |
| GTAACAGCGTCTCCATCATC+CGG | 0.439205 | 5.2:-10416157 | MS.gene74500:CDS |
| GGGGCTTGGGAGTTTGGCAC+AGG | 0.442466 | 5.2:-10415888 | MS.gene74500:CDS |
| AGACGCTGTTACGGAGTTTG+TGG | 0.442900 | 5.2:+10416168 | None:intergenic |
| AGTCATGACTTAGAACCTTC+AGG | 0.448927 | 5.2:+10415794 | None:intergenic |
| ATATTCCTCTGTTTGGGATA+CGG | 0.449150 | 5.2:-10416060 | MS.gene74500:CDS |
| CACGTTGAAAGGAGAGAGTT+GGG | 0.450299 | 5.2:+10416233 | None:intergenic |
| GGATTTGAATCCGCGGAGAA+AGG | 0.475547 | 5.2:-10415989 | MS.gene74500:CDS |
| TGAGAAGGCGGAACTAGGAT+TGG | 0.477543 | 5.2:-10415942 | MS.gene74500:CDS |
| AGCTCCCTTCGCCCGGATGA+TGG | 0.478842 | 5.2:+10416145 | None:intergenic |
| AGGATTGGAGGAGGCGGAGA+AGG | 0.488587 | 5.2:-10415927 | MS.gene74500:CDS |
| GGACTTGAGAAGGCGGAACT+AGG | 0.502229 | 5.2:-10415947 | MS.gene74500:CDS |
| TGAATCCGCGGAGAAAGGAT+TGG | 0.503269 | 5.2:-10415984 | MS.gene74500:CDS |
| GCACAGGCGATTGTGGTGGC+CGG | 0.505389 | 5.2:-10415872 | MS.gene74500:CDS |
| GCTGAGGAGATTGTGAAGGA+AGG | 0.507532 | 5.2:-10416010 | MS.gene74500:CDS |
| CGTCTCCATCATCCGGGCGA+AGG | 0.507772 | 5.2:-10416150 | MS.gene74500:CDS |
| GCGGAGAAGGAGATAGAGAC+GGG | 0.509765 | 5.2:-10415914 | MS.gene74500:CDS |
| GTCTATTGCTTTAACGGCAA+GGG | 0.515051 | 5.2:-10416104 | MS.gene74500:CDS |
| ACACGTTGAAAGGAGAGAGT+TGG | 0.515766 | 5.2:+10416232 | None:intergenic |
| AAACAGCAGCTCCCTTCGCC+CGG | 0.527420 | 5.2:+10416138 | None:intergenic |
| GTCTCCATCATCCGGGCGAA+GGG | 0.529471 | 5.2:-10416149 | MS.gene74500:CDS |
| GGATGATGGAGACGCTGTTA+CGG | 0.541392 | 5.2:+10416159 | None:intergenic |
| GGAACTAGGATTGGAGGAGG+CGG | 0.541402 | 5.2:-10415933 | MS.gene74500:CDS |
| CGTCTATTGCTTTAACGGCA+AGG | 0.541905 | 5.2:-10416105 | MS.gene74500:CDS |
| GAGTTTGGCACAGGCGATTG+TGG | 0.547955 | 5.2:-10415879 | MS.gene74500:CDS |
| GAAGGCGGAACTAGGATTGG+AGG | 0.553823 | 5.2:-10415939 | MS.gene74500:CDS |
| GATTGTGGTGGCCGGAGCAG+AGG | 0.555003 | 5.2:-10415864 | MS.gene74500:CDS |
| GCGAGCGATGACACGTTGAA+AGG | 0.558528 | 5.2:+10416222 | None:intergenic |
| AAAAGCTGAGGAGATTGTGA+AGG | 0.558586 | 5.2:-10416014 | MS.gene74500:CDS |
| GGAGCTGCTGTTTCTTCTGA+CGG | 0.565357 | 5.2:-10416128 | MS.gene74500:CDS |
| TTTGGCACAGGCGATTGTGG+TGG | 0.576692 | 5.2:-10415876 | MS.gene74500:CDS |
| GGAATCGGTGTCTATGGCGC+AGG | 0.576983 | 5.2:-10416083 | MS.gene74500:CDS |
| GGCGGAGAAGGAGATAGAGA+CGG | 0.579933 | 5.2:-10415915 | MS.gene74500:CDS |
| CGCGGAGAAAGGATTGGAGA+CGG | 0.585103 | 5.2:-10415978 | MS.gene74500:CDS |
| TAACAGCGTCTCCATCATCC+GGG | 0.601839 | 5.2:-10416156 | MS.gene74500:CDS |
| GTGTGTCGTGAAGCAATGGT+TGG | 0.601927 | 5.2:-10416274 | None:intergenic |
| GGCGGAACTAGGATTGGAGG+AGG | 0.605352 | 5.2:-10415936 | MS.gene74500:CDS |
| CGGAGAAGGAGATAGAGACG+GGG | 0.636283 | 5.2:-10415913 | MS.gene74500:CDS |
| TGTGTCGTGAAGCAATGGTT+GGG | 0.636465 | 5.2:-10416273 | None:intergenic |
| GGAGAAAGGATTGGAGACGG+CGG | 0.673797 | 5.2:-10415975 | MS.gene74500:CDS |
| CGTCTCCAATCCTTTCTCCG+CGG | 0.675587 | 5.2:+10415979 | None:intergenic |
| CTCTTCCGTATCCCAAACAG+AGG | 0.678236 | 5.2:+10416055 | None:intergenic |
| GGAGAAGGAGATAGAGACGG+GGG | 0.684188 | 5.2:-10415912 | MS.gene74500:CDS |
| GAAGGAAGGATTTGAATCCG+CGG | 0.697190 | 5.2:-10415996 | MS.gene74500:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATTCCTCTGTTTGGGATA+CGG | - | chr5.2:10415995-10416014 | MS.gene74500:CDS | 35.0% |
| AAAAGCTGAGGAGATTGTGA+AGG | - | chr5.2:10416041-10416060 | MS.gene74500:CDS | 40.0% | |
| GAAAGCAGAAGAAAAAGCTG+AGG | - | chr5.2:10416029-10416048 | MS.gene74500:CDS | 40.0% | |
| GTCTATTGCTTTAACGGCAA+GGG | - | chr5.2:10415951-10415970 | MS.gene74500:CDS | 40.0% | |
| !! | TTTTGGGCTTTTGGTTGCTT+CGG | - | chr5.2:10416215-10416234 | MS.gene74500:CDS | 40.0% |
| !!! | AATGGTATTTTGGGACCTGA+AGG | - | chr5.2:10416246-10416265 | MS.gene74500:CDS | 40.0% |
| ACACGTTGAAAGGAGAGAGT+TGG | + | chr5.2:10415826-10415845 | None:intergenic | 45.0% | |
| CACGTTGAAAGGAGAGAGTT+GGG | + | chr5.2:10415825-10415844 | None:intergenic | 45.0% | |
| CGCAGGATATTCCTCTGTTT+GGG | - | chr5.2:10415989-10416008 | MS.gene74500:CDS | 45.0% | |
| CGTCTATTGCTTTAACGGCA+AGG | - | chr5.2:10415950-10415969 | MS.gene74500:CDS | 45.0% | |
| GAAGGAAGGATTTGAATCCG+CGG | - | chr5.2:10416059-10416078 | MS.gene74500:CDS | 45.0% | |
| GACGGCGTCTATTGCTTTAA+CGG | - | chr5.2:10415945-10415964 | MS.gene74500:CDS | 45.0% | |
| GGAAAAAGGACTTGAGAAGG+CGG | - | chr5.2:10416101-10416120 | MS.gene74500:CDS | 45.0% | |
| TGCTTTAACGGCAAGGGAAT+CGG | - | chr5.2:10415957-10415976 | MS.gene74500:CDS | 45.0% | |
| ! | GCGGTGGTGAATGGTATTTT+GGG | - | chr5.2:10416237-10416256 | MS.gene74500:CDS | 45.0% |
| !! | AGCAGAGGTTTTTGGGCTTT+TGG | - | chr5.2:10416206-10416225 | MS.gene74500:CDS | 45.0% |
| AAGCCCAAAAACCTCTGCTC+CGG | + | chr5.2:10416205-10416224 | None:intergenic | 50.0% | |
| AGACGCTGTTACGGAGTTTG+TGG | + | chr5.2:10415890-10415909 | None:intergenic | 50.0% | |
| CTCTTCCGTATCCCAAACAG+AGG | + | chr5.2:10416003-10416022 | None:intergenic | 50.0% | |
| GCGCAGGATATTCCTCTGTT+TGG | - | chr5.2:10415988-10416007 | MS.gene74500:CDS | 50.0% | |
| GCTGAGGAGATTGTGAAGGA+AGG | - | chr5.2:10416045-10416064 | MS.gene74500:CDS | 50.0% | |
| GGATTTGAATCCGCGGAGAA+AGG | - | chr5.2:10416066-10416085 | MS.gene74500:CDS | 50.0% | |
| GGCGGAAAAAGGACTTGAGA+AGG | - | chr5.2:10416098-10416117 | MS.gene74500:CDS | 50.0% | |
| GTAACAGCGTCTCCATCATC+CGG | - | chr5.2:10415898-10415917 | MS.gene74500:CDS | 50.0% | |
| TAACAGCGTCTCCATCATCC+GGG | - | chr5.2:10415899-10415918 | MS.gene74500:CDS | 50.0% | |
| TGAATCCGCGGAGAAAGGAT+TGG | - | chr5.2:10416071-10416090 | MS.gene74500:CDS | 50.0% | |
| TGAGAAGGCGGAACTAGGAT+TGG | - | chr5.2:10416113-10416132 | MS.gene74500:CDS | 50.0% | |
| ! | GGCGGTGGTGAATGGTATTT+TGG | - | chr5.2:10416236-10416255 | MS.gene74500:CDS | 50.0% |
| !! | GGAGCTGCTGTTTCTTCTGA+CGG | - | chr5.2:10415927-10415946 | MS.gene74500:CDS | 50.0% |
| !! | GGATGATGGAGACGCTGTTA+CGG | + | chr5.2:10415899-10415918 | None:intergenic | 50.0% |
| AAGGAGATAGAGACGGGGGT+GGG | - | chr5.2:10416147-10416166 | MS.gene74500:CDS | 55.0% | |
| CGCGGAGAAAGGATTGGAGA+CGG | - | chr5.2:10416077-10416096 | MS.gene74500:CDS | 55.0% | |
| CGGAGAAGGAGATAGAGACG+GGG | - | chr5.2:10416142-10416161 | MS.gene74500:CDS | 55.0% | |
| CGTCTCCAATCCTTTCTCCG+CGG | + | chr5.2:10416079-10416098 | None:intergenic | 55.0% | |
| GAAGGCGGAACTAGGATTGG+AGG | - | chr5.2:10416116-10416135 | MS.gene74500:CDS | 55.0% | |
| GAGTTTGGCACAGGCGATTG+TGG | - | chr5.2:10416176-10416195 | MS.gene74500:CDS | 55.0% | |
| GCGAGCGATGACACGTTGAA+AGG | + | chr5.2:10415836-10415855 | None:intergenic | 55.0% | |
| GCGGAGAAGGAGATAGAGAC+GGG | - | chr5.2:10416141-10416160 | MS.gene74500:CDS | 55.0% | |
| GGAACTAGGATTGGAGGAGG+CGG | - | chr5.2:10416122-10416141 | MS.gene74500:CDS | 55.0% | |
| GGACTTGAGAAGGCGGAACT+AGG | - | chr5.2:10416108-10416127 | MS.gene74500:CDS | 55.0% | |
| GGAGAAAGGATTGGAGACGG+CGG | - | chr5.2:10416080-10416099 | MS.gene74500:CDS | 55.0% | |
| GGAGAAGGAGATAGAGACGG+GGG | - | chr5.2:10416143-10416162 | MS.gene74500:CDS | 55.0% | |
| GGATTGGAGACGGCGGAAAA+AGG | - | chr5.2:10416087-10416106 | MS.gene74500:CDS | 55.0% | |
| GGCGGAGAAGGAGATAGAGA+CGG | - | chr5.2:10416140-10416159 | MS.gene74500:CDS | 55.0% | |
| TTTGGCACAGGCGATTGTGG+TGG | - | chr5.2:10416179-10416198 | MS.gene74500:CDS | 55.0% | |
| ! | GGCAAGGGAATCGGTGTCTA+TGG | - | chr5.2:10415966-10415985 | MS.gene74500:CDS | 55.0% |
| ! | TGGCCGGAGCAGAGGTTTTT+GGG | - | chr5.2:10416199-10416218 | MS.gene74500:CDS | 55.0% |
| !! | TGGGCTTTTGGTTGCTTCGG+CGG | - | chr5.2:10416218-10416237 | MS.gene74500:CDS | 55.0% |
| AAACAGCAGCTCCCTTCGCC+CGG | + | chr5.2:10415920-10415939 | None:intergenic | 60.0% | |
| AGGAGATAGAGACGGGGGTG+GGG | - | chr5.2:10416148-10416167 | MS.gene74500:CDS | 60.0% | |
| AGGATTGGAGGAGGCGGAGA+AGG | - | chr5.2:10416128-10416147 | MS.gene74500:CDS | 60.0% | |
| GAAGGAGATAGAGACGGGGG+TGG | - | chr5.2:10416146-10416165 | MS.gene74500:CDS | 60.0% | |
| GGCGGAACTAGGATTGGAGG+AGG | - | chr5.2:10416119-10416138 | MS.gene74500:CDS | 60.0% | |
| GTCTCCATCATCCGGGCGAA+GGG | - | chr5.2:10415906-10415925 | MS.gene74500:CDS | 60.0% | |
| GTTGCTTCGGCGGTGGTGAA+TGG | - | chr5.2:10416228-10416247 | MS.gene74500:CDS | 60.0% | |
| ! | GGAATCGGTGTCTATGGCGC+AGG | - | chr5.2:10415972-10415991 | MS.gene74500:CDS | 60.0% |
| ! | GTGGCCGGAGCAGAGGTTTT+TGG | - | chr5.2:10416198-10416217 | MS.gene74500:CDS | 60.0% |
| !! | GCTTTTGGTTGCTTCGGCGG+TGG | - | chr5.2:10416221-10416240 | MS.gene74500:CDS | 60.0% |
| AGCTCCCTTCGCCCGGATGA+TGG | + | chr5.2:10415913-10415932 | None:intergenic | 65.0% | |
| ATAGAGACGGGGGTGGGGCT+TGG | - | chr5.2:10416153-10416172 | MS.gene74500:CDS | 65.0% | |
| CGTCTCCATCATCCGGGCGA+AGG | - | chr5.2:10415905-10415924 | MS.gene74500:CDS | 65.0% | |
| GATTGTGGTGGCCGGAGCAG+AGG | - | chr5.2:10416191-10416210 | MS.gene74500:CDS | 65.0% | |
| GCACAGGCGATTGTGGTGGC+CGG | - | chr5.2:10416183-10416202 | MS.gene74500:CDS | 65.0% | |
| GGGGCTTGGGAGTTTGGCAC+AGG | - | chr5.2:10416167-10416186 | MS.gene74500:CDS | 65.0% | |
| TAGAGACGGGGGTGGGGCTT+GGG | - | chr5.2:10416154-10416173 | MS.gene74500:CDS | 65.0% | |
| GGGGGTGGGGCTTGGGAGTT+TGG | - | chr5.2:10416161-10416180 | MS.gene74500:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 10415796 | 10416281 | 10415796 | ID=MS.gene74500 |
| chr5.2 | mRNA | 10415796 | 10416281 | 10415796 | ID=MS.gene74500.t1;Parent=MS.gene74500 |
| chr5.2 | exon | 10415796 | 10416281 | 10415796 | ID=MS.gene74500.t1.exon1;Parent=MS.gene74500.t1 |
| chr5.2 | CDS | 10415796 | 10416281 | 10415796 | ID=cds.MS.gene74500.t1;Parent=MS.gene74500.t1 |
| Gene Sequence |
| Protein sequence |
