Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79232.t1 | XP_003601984.1 | 99.4 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 4.80E-86 | 327 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79232.t1 | Q9LX15 | 46.9 | 162 | 81 | 3 | 1 | 159 | 1 | 160 | 8.5e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79232.t1 | G7J4B2 | 99.4 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 3.5e-86 | 327.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050329 | MS.gene79232 | 0.823836 | 1.12E-53 | -1.69E-46 |
| MS.gene051158 | MS.gene79232 | 0.818759 | 1.66E-52 | -1.69E-46 |
| MS.gene051552 | MS.gene79232 | 0.800412 | 1.46E-48 | -1.69E-46 |
| MS.gene051996 | MS.gene79232 | 0.853446 | 2.38E-61 | -1.69E-46 |
| MS.gene052223 | MS.gene79232 | 0.956017 | 7.91E-114 | -1.69E-46 |
| MS.gene05233 | MS.gene79232 | 0.819601 | 1.07E-52 | -1.69E-46 |
| MS.gene052705 | MS.gene79232 | 0.814119 | 1.81E-51 | -1.69E-46 |
| MS.gene052706 | MS.gene79232 | 0.813489 | 2.49E-51 | -1.69E-46 |
| MS.gene054138 | MS.gene79232 | 0.802699 | 4.96E-49 | -1.69E-46 |
| MS.gene054277 | MS.gene79232 | 0.836113 | 1.13E-56 | -1.69E-46 |
| MS.gene054742 | MS.gene79232 | 0.855506 | 6.03E-62 | -1.69E-46 |
| MS.gene055157 | MS.gene79232 | 0.800095 | 1.69E-48 | -1.69E-46 |
| MS.gene055601 | MS.gene79232 | 0.813909 | 2.02E-51 | -1.69E-46 |
| MS.gene056330 | MS.gene79232 | 0.870768 | 1.14E-66 | -1.69E-46 |
| MS.gene056680 | MS.gene79232 | 0.845057 | 5.15E-59 | -1.69E-46 |
| MS.gene056690 | MS.gene79232 | 0.854803 | 9.65E-62 | -1.69E-46 |
| MS.gene056691 | MS.gene79232 | 0.84904 | 4.17E-60 | -1.69E-46 |
| MS.gene056899 | MS.gene79232 | 0.802729 | 4.89E-49 | -1.69E-46 |
| MS.gene056920 | MS.gene79232 | 0.84682 | 1.71E-59 | -1.69E-46 |
| MS.gene056923 | MS.gene79232 | 0.84764 | 1.02E-59 | -1.69E-46 |
| MS.gene056924 | MS.gene79232 | 0.844202 | 8.75E-59 | -1.69E-46 |
| MS.gene056925 | MS.gene79232 | 0.823587 | 1.28E-53 | -1.69E-46 |
| MS.gene059328 | MS.gene79232 | 0.809717 | 1.65E-50 | -1.69E-46 |
| MS.gene059891 | MS.gene79232 | 0.813693 | 2.25E-51 | -1.69E-46 |
| MS.gene059892 | MS.gene79232 | 0.806944 | 6.43E-50 | -1.69E-46 |
| MS.gene060087 | MS.gene79232 | 0.8289 | 6.95E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79232.t1 | MTR_3g087540 | 99.375 | 160 | 1 | 0 | 1 | 160 | 1 | 160 | 3.90e-119 | 333 |
| MS.gene79232.t1 | MTR_3g073990 | 39.860 | 143 | 78 | 3 | 16 | 152 | 13 | 153 | 4.66e-31 | 110 |
| MS.gene79232.t1 | MTR_2g016020 | 34.848 | 132 | 78 | 5 | 25 | 152 | 28 | 155 | 1.78e-22 | 88.2 |
| MS.gene79232.t1 | MTR_2g077940 | 27.160 | 162 | 99 | 4 | 4 | 151 | 8 | 164 | 1.74e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79232.t1 | AT5G10130 | 46.914 | 162 | 81 | 3 | 1 | 159 | 1 | 160 | 3.17e-45 | 146 |
| MS.gene79232.t1 | AT4G08685 | 40.994 | 161 | 84 | 4 | 5 | 159 | 4 | 159 | 5.11e-36 | 122 |
| MS.gene79232.t1 | AT1G78040 | 36.095 | 169 | 92 | 5 | 1 | 159 | 1 | 163 | 5.76e-34 | 117 |
| MS.gene79232.t1 | AT1G78040 | 36.095 | 169 | 92 | 5 | 1 | 159 | 1 | 163 | 5.76e-34 | 117 |
| MS.gene79232.t1 | AT1G78040 | 36.095 | 169 | 92 | 5 | 1 | 159 | 1 | 163 | 5.76e-34 | 117 |
| MS.gene79232.t1 | AT1G29140 | 35.669 | 157 | 92 | 3 | 4 | 152 | 6 | 161 | 1.14e-26 | 99.0 |
| MS.gene79232.t1 | AT5G45880 | 39.024 | 123 | 70 | 3 | 25 | 143 | 35 | 156 | 3.91e-23 | 90.1 |
| MS.gene79232.t1 | AT4G18596 | 37.705 | 122 | 71 | 3 | 25 | 142 | 33 | 153 | 2.57e-22 | 87.8 |
| MS.gene79232.t1 | AT4G18596 | 37.705 | 122 | 71 | 3 | 25 | 142 | 79 | 199 | 6.42e-22 | 88.2 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGATTGTGTTGCTGTTAA+TGG | 0.278593 | 3.1:-70413371 | MS.gene79232:CDS |
| AATAAACACAACCTTTGATA+TGG | 0.316996 | 3.1:+70413302 | None:intergenic |
| GTGTATTACACGATGATATT+GGG | 0.373900 | 3.1:+70412852 | None:intergenic |
| GGTGTATTACACGATGATAT+TGG | 0.406710 | 3.1:+70412851 | None:intergenic |
| CACTGGAACATACAACATAA+TGG | 0.429786 | 3.1:-70412924 | MS.gene79232:CDS |
| CGTCCATAGTCTTCCTGTCT+TGG | 0.431661 | 3.1:+70412978 | None:intergenic |
| ACAAGTAATATTTGAGGAGT+TGG | 0.437901 | 3.1:+70412705 | None:intergenic |
| AAATGCTATGGGTTACTTCA+AGG | 0.456909 | 3.1:-70412753 | MS.gene79232:CDS |
| TAGGTGCAAAGGTGGGAATT+CGG | 0.460306 | 3.1:-70413003 | MS.gene79232:CDS |
| TGGGAATTCGGTGCCAAGAC+AGG | 0.469322 | 3.1:-70412991 | MS.gene79232:CDS |
| TATTGTGATACTTGCCGTGC+TGG | 0.471219 | 3.1:-70413283 | MS.gene79232:CDS |
| GTGCCAAGACAGGAAGACTA+TGG | 0.475430 | 3.1:-70412981 | MS.gene79232:CDS |
| TCTTCACTATGCAAATGCTA+TGG | 0.481660 | 3.1:-70412765 | MS.gene79232:CDS |
| GGAGGATAGAGCTGAGGGAA+AGG | 0.482593 | 3.1:+70413347 | None:intergenic |
| TAGCATTTGCATAGTGAAGA+TGG | 0.484441 | 3.1:+70412768 | None:intergenic |
| GATCTGTTACGCCCAGGGTC+CGG | 0.486304 | 3.1:+70412830 | None:intergenic |
| AACATAGCTGTGGCAAGTAA+AGG | 0.486675 | 3.1:+70413325 | None:intergenic |
| ACATAGCTGTGGCAAGTAAA+GGG | 0.489307 | 3.1:+70413326 | None:intergenic |
| GAGGATAGAGCTGAGGGAAA+GGG | 0.496587 | 3.1:+70413348 | None:intergenic |
| AGGAGTTGGTGGCAATATTG+AGG | 0.508425 | 3.1:+70412719 | None:intergenic |
| AATCAGACAAGTAATATTTG+AGG | 0.510401 | 3.1:+70412699 | None:intergenic |
| TGCTGGATCTGTTACGCCCA+GGG | 0.512778 | 3.1:+70412825 | None:intergenic |
| AGTAATATTTGAGGAGTTGG+TGG | 0.513246 | 3.1:+70412708 | None:intergenic |
| GTAGTGAGTGAGCACAATGC+TGG | 0.521929 | 3.1:+70412808 | None:intergenic |
| ATGCTGGATCTGTTACGCCC+AGG | 0.535067 | 3.1:+70412824 | None:intergenic |
| CTTCACTATGCAAATGCTAT+GGG | 0.535954 | 3.1:-70412764 | MS.gene79232:CDS |
| TCGTGTAATACACCGGACCC+TGG | 0.543647 | 3.1:-70412842 | MS.gene79232:CDS |
| TTGCATAGTGAAGATGGTTG+AGG | 0.549905 | 3.1:+70412774 | None:intergenic |
| TTCTTGATTAGGTGCAAAGG+TGG | 0.550705 | 3.1:-70413011 | MS.gene79232:intron |
| CGTGTAATACACCGGACCCT+GGG | 0.556675 | 3.1:-70412841 | MS.gene79232:CDS |
| TGGTGGCAATATTGAGGCAA+AGG | 0.557338 | 3.1:+70412725 | None:intergenic |
| CATTATGTTGTATGTTCCAG+TGG | 0.561706 | 3.1:+70412925 | None:intergenic |
| CATTGCTAATCATCATGGCA+AGG | 0.587013 | 3.1:-70413390 | None:intergenic |
| CGATTCTTGATTAGGTGCAA+AGG | 0.587702 | 3.1:-70413014 | MS.gene79232:intron |
| ACAGCTATGTTCCATATCAA+AGG | 0.587960 | 3.1:-70413313 | MS.gene79232:CDS |
| TAAAGGGAGGATAGAGCTGA+GGG | 0.597004 | 3.1:+70413342 | None:intergenic |
| GTAAAGGGAGGATAGAGCTG+AGG | 0.612679 | 3.1:+70413341 | None:intergenic |
| GAAGGAGTGACAGATTCCAC+TGG | 0.617675 | 3.1:-70412941 | MS.gene79232:CDS |
| TTGGATGTAAAATGTGGCGT+TGG | 0.634933 | 3.1:+70413252 | None:intergenic |
| CGTTGGTTTCGAATCCAGCA+CGG | 0.637233 | 3.1:+70413269 | None:intergenic |
| TCTTGATTAGGTGCAAAGGT+GGG | 0.642740 | 3.1:-70413010 | MS.gene79232:intron |
| AATATCATCGTGTAATACAC+CGG | 0.667061 | 3.1:-70412849 | MS.gene79232:CDS |
| TTTACCTTGGATGTAAAATG+TGG | 0.673598 | 3.1:+70413246 | None:intergenic |
| TTTGATATGGAACATAGCTG+TGG | 0.697489 | 3.1:+70413315 | None:intergenic |
| TAGCTGTGGCAAGTAAAGGG+AGG | 0.702643 | 3.1:+70413329 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTAAAAAATTTACGTTA+AGG | + | chr3.1:70412850-70412869 | None:intergenic | 15.0% |
| ! | AATAAACACAACCTTTGATA+TGG | + | chr3.1:70412765-70412784 | None:intergenic | 25.0% |
| ! | AATCAGACAAGTAATATTTG+AGG | + | chr3.1:70413368-70413387 | None:intergenic | 25.0% |
| AATATCATCGTGTAATACAC+CGG | - | chr3.1:70413215-70413234 | MS.gene79232:intron | 30.0% | |
| ACAAGTAATATTTGAGGAGT+TGG | + | chr3.1:70413362-70413381 | None:intergenic | 30.0% | |
| GTGTATTACACGATGATATT+GGG | + | chr3.1:70413215-70413234 | None:intergenic | 30.0% | |
| TATTGTGACGATTCTTGATT+AGG | - | chr3.1:70413042-70413061 | MS.gene79232:intron | 30.0% | |
| TGTAAAATGTACACATGATC+AGG | + | chr3.1:70412921-70412940 | None:intergenic | 30.0% | |
| TTTACCTTGGATGTAAAATG+TGG | + | chr3.1:70412821-70412840 | None:intergenic | 30.0% | |
| ! | CAGTTTTGCAAATATTGTCA+TGG | + | chr3.1:70413179-70413198 | None:intergenic | 30.0% |
| ACAGCTATGTTCCATATCAA+AGG | - | chr3.1:70412751-70412770 | MS.gene79232:CDS | 35.0% | |
| AGTAATATTTGAGGAGTTGG+TGG | + | chr3.1:70413359-70413378 | None:intergenic | 35.0% | |
| CACTGGAACATACAACATAA+TGG | - | chr3.1:70413140-70413159 | MS.gene79232:intron | 35.0% | |
| CATTATGTTGTATGTTCCAG+TGG | + | chr3.1:70413142-70413161 | None:intergenic | 35.0% | |
| CTTCACTATGCAAATGCTAT+GGG | - | chr3.1:70413300-70413319 | MS.gene79232:CDS | 35.0% | |
| GGTGTATTACACGATGATAT+TGG | + | chr3.1:70413216-70413235 | None:intergenic | 35.0% | |
| GTTAAGGTGTGTATTTACCT+TGG | + | chr3.1:70412834-70412853 | None:intergenic | 35.0% | |
| TAGCATTTGCATAGTGAAGA+TGG | + | chr3.1:70413299-70413318 | None:intergenic | 35.0% | |
| TCTTCACTATGCAAATGCTA+TGG | - | chr3.1:70413299-70413318 | MS.gene79232:CDS | 35.0% | |
| TTTGATATGGAACATAGCTG+TGG | + | chr3.1:70412752-70412771 | None:intergenic | 35.0% | |
| ! | AAATGCTATGGGTTACTTCA+AGG | - | chr3.1:70413311-70413330 | MS.gene79232:CDS | 35.0% |
| ! | AAGGATTGTGTTGCTGTTAA+TGG | - | chr3.1:70412693-70412712 | MS.gene79232:CDS | 35.0% |
| ! | TATGGACGAAGTTTTCTACA+CGG | - | chr3.1:70413101-70413120 | MS.gene79232:intron | 35.0% |
| AACATAGCTGTGGCAAGTAA+AGG | + | chr3.1:70412742-70412761 | None:intergenic | 40.0% | |
| ACATAGCTGTGGCAAGTAAA+GGG | + | chr3.1:70412741-70412760 | None:intergenic | 40.0% | |
| CGATTCTTGATTAGGTGCAA+AGG | - | chr3.1:70413050-70413069 | MS.gene79232:intron | 40.0% | |
| GTGCTCACTCACTACAAAAA+TGG | - | chr3.1:70413264-70413283 | MS.gene79232:CDS | 40.0% | |
| TCTTGATTAGGTGCAAAGGT+GGG | - | chr3.1:70413054-70413073 | MS.gene79232:intron | 40.0% | |
| TTCTTGATTAGGTGCAAAGG+TGG | - | chr3.1:70413053-70413072 | MS.gene79232:intron | 40.0% | |
| TTGCATAGTGAAGATGGTTG+AGG | + | chr3.1:70413293-70413312 | None:intergenic | 40.0% | |
| TTGGATGTAAAATGTGGCGT+TGG | + | chr3.1:70412815-70412834 | None:intergenic | 40.0% | |
| ! | AACGCCACATTTTACATCCA+AGG | - | chr3.1:70412814-70412833 | MS.gene79232:CDS | 40.0% |
| TAAAGGGAGGATAGAGCTGA+GGG | + | chr3.1:70412725-70412744 | None:intergenic | 45.0% | |
| TAGGTGCAAAGGTGGGAATT+CGG | - | chr3.1:70413061-70413080 | MS.gene79232:intron | 45.0% | |
| TATTGTGATACTTGCCGTGC+TGG | - | chr3.1:70412781-70412800 | MS.gene79232:CDS | 45.0% | |
| TGGTGGCAATATTGAGGCAA+AGG | + | chr3.1:70413342-70413361 | None:intergenic | 45.0% | |
| ! | GACGAAGTTTTCTACACGGA+AGG | - | chr3.1:70413105-70413124 | MS.gene79232:intron | 45.0% |
| !! | AGGAGTTGGTGGCAATATTG+AGG | + | chr3.1:70413348-70413367 | None:intergenic | 45.0% |
| CGTCCATAGTCTTCCTGTCT+TGG | + | chr3.1:70413089-70413108 | None:intergenic | 50.0% | |
| GAAGGAGTGACAGATTCCAC+TGG | - | chr3.1:70413123-70413142 | MS.gene79232:intron | 50.0% | |
| GAGGATAGAGCTGAGGGAAA+GGG | + | chr3.1:70412719-70412738 | None:intergenic | 50.0% | |
| GTAAAGGGAGGATAGAGCTG+AGG | + | chr3.1:70412726-70412745 | None:intergenic | 50.0% | |
| GTAGTGAGTGAGCACAATGC+TGG | + | chr3.1:70413259-70413278 | None:intergenic | 50.0% | |
| GTGCCAAGACAGGAAGACTA+TGG | - | chr3.1:70413083-70413102 | MS.gene79232:intron | 50.0% | |
| TAGCTGTGGCAAGTAAAGGG+AGG | + | chr3.1:70412738-70412757 | None:intergenic | 50.0% | |
| !! | CGTTGGTTTCGAATCCAGCA+CGG | + | chr3.1:70412798-70412817 | None:intergenic | 50.0% |
| ATGCTGGATCTGTTACGCCC+AGG | + | chr3.1:70413243-70413262 | None:intergenic | 55.0% | |
| CGTGTAATACACCGGACCCT+GGG | - | chr3.1:70413223-70413242 | MS.gene79232:intron | 55.0% | |
| GGAGGATAGAGCTGAGGGAA+AGG | + | chr3.1:70412720-70412739 | None:intergenic | 55.0% | |
| TCGTGTAATACACCGGACCC+TGG | - | chr3.1:70413222-70413241 | MS.gene79232:intron | 55.0% | |
| TGCTGGATCTGTTACGCCCA+GGG | + | chr3.1:70413242-70413261 | None:intergenic | 55.0% | |
| !! | TGGGAATTCGGTGCCAAGAC+AGG | - | chr3.1:70413073-70413092 | MS.gene79232:intron | 55.0% |
| GATCTGTTACGCCCAGGGTC+CGG | + | chr3.1:70413237-70413256 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 70412688 | 70413398 | 70412688 | ID=MS.gene79232 |
| chr3.1 | mRNA | 70412688 | 70413398 | 70412688 | ID=MS.gene79232.t1;Parent=MS.gene79232 |
| chr3.1 | exon | 70413251 | 70413398 | 70413251 | ID=MS.gene79232.t1.exon1;Parent=MS.gene79232.t1 |
| chr3.1 | CDS | 70413251 | 70413398 | 70413251 | ID=cds.MS.gene79232.t1;Parent=MS.gene79232.t1 |
| chr3.1 | exon | 70412688 | 70413022 | 70412688 | ID=MS.gene79232.t1.exon2;Parent=MS.gene79232.t1 |
| chr3.1 | CDS | 70412688 | 70413022 | 70412688 | ID=cds.MS.gene79232.t1;Parent=MS.gene79232.t1 |
| Gene Sequence |
| Protein sequence |
