Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79445.t1 | RHN49252.1 | 90.6 | 159 | 15 | 0 | 1 | 159 | 191 | 349 | 1.60E-81 | 312 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79445.t1 | I6YGS0 | 35.0 | 117 | 66 | 2 | 42 | 156 | 208 | 316 | 8.5e-15 | 81.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79445.t1 | A2Q324 | 90.6 | 159 | 15 | 0 | 1 | 159 | 161 | 319 | 1.2e-81 | 312.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene79445 | 0.81601 | 6.89E-52 | -1.69E-46 |
| MS.gene049634 | MS.gene79445 | 0.818114 | 2.32E-52 | -1.69E-46 |
| MS.gene050380 | MS.gene79445 | 0.80178 | 7.66E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene79445 | 0.808039 | 3.77E-50 | -1.69E-46 |
| MS.gene050647 | MS.gene79445 | 0.802062 | 6.71E-49 | -1.69E-46 |
| MS.gene05079 | MS.gene79445 | 0.805964 | 1.03E-49 | -1.69E-46 |
| MS.gene051131 | MS.gene79445 | 0.800241 | 1.58E-48 | -1.69E-46 |
| MS.gene051138 | MS.gene79445 | 0.800384 | 1.48E-48 | -1.69E-46 |
| MS.gene051158 | MS.gene79445 | 0.804137 | 2.50E-49 | -1.69E-46 |
| MS.gene051632 | MS.gene79445 | 0.834816 | 2.41E-56 | -1.69E-46 |
| MS.gene052211 | MS.gene79445 | 0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene79445 | 0.810934 | 9.00E-51 | -1.69E-46 |
| MS.gene052805 | MS.gene79445 | 0.801372 | 9.29E-49 | -1.69E-46 |
| MS.gene053033 | MS.gene79445 | 0.800712 | 1.27E-48 | -1.69E-46 |
| MS.gene053406 | MS.gene79445 | 0.823447 | 1.38E-53 | -1.69E-46 |
| MS.gene053420 | MS.gene79445 | 0.820704 | 5.96E-53 | -1.69E-46 |
| MS.gene054181 | MS.gene79445 | 0.827397 | 1.60E-54 | -1.69E-46 |
| MS.gene054742 | MS.gene79445 | 0.805282 | 1.44E-49 | -1.69E-46 |
| MS.gene055154 | MS.gene79445 | 0.836491 | 9.07E-57 | -1.69E-46 |
| MS.gene055959 | MS.gene79445 | 0.822379 | 2.45E-53 | -1.69E-46 |
| MS.gene057261 | MS.gene79445 | 0.806406 | 8.36E-50 | -1.69E-46 |
| MS.gene057544 | MS.gene79445 | 0.80984 | 1.55E-50 | -1.69E-46 |
| MS.gene058067 | MS.gene79445 | 0.801451 | 8.95E-49 | -1.69E-46 |
| MS.gene058093 | MS.gene79445 | 0.802206 | 6.26E-49 | -1.69E-46 |
| MS.gene058218 | MS.gene79445 | 0.813376 | 2.64E-51 | -1.69E-46 |
| MS.gene059037 | MS.gene79445 | 0.806406 | 8.36E-50 | -1.69E-46 |
| MS.gene059038 | MS.gene79445 | 0.802592 | 5.21E-49 | -1.69E-46 |
| MS.gene059402 | MS.gene79445 | 0.831207 | 1.90E-55 | -1.69E-46 |
| MS.gene059953 | MS.gene79445 | 0.809814 | 1.57E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene79445 | 0.843188 | 1.63E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79445.t1 | MTR_7g113050 | 90.566 | 159 | 15 | 0 | 1 | 159 | 161 | 319 | 4.63e-106 | 306 |
| MS.gene79445.t1 | MTR_7g113030 | 89.937 | 159 | 16 | 0 | 1 | 159 | 161 | 319 | 5.54e-105 | 303 |
| MS.gene79445.t1 | MTR_7g112615 | 89.937 | 159 | 16 | 0 | 1 | 159 | 161 | 319 | 5.94e-104 | 300 |
| MS.gene79445.t1 | MTR_7g112620 | 88.679 | 159 | 18 | 0 | 1 | 159 | 161 | 319 | 2.01e-102 | 296 |
| MS.gene79445.t1 | MTR_7g113020 | 86.792 | 159 | 21 | 0 | 1 | 159 | 161 | 319 | 1.61e-100 | 291 |
| MS.gene79445.t1 | MTR_4g005160 | 70.440 | 159 | 46 | 1 | 2 | 159 | 164 | 322 | 5.82e-81 | 242 |
| MS.gene79445.t1 | MTR_7g112960 | 66.460 | 161 | 50 | 2 | 1 | 159 | 161 | 319 | 4.26e-72 | 219 |
| MS.gene79445.t1 | MTR_7g112963 | 67.081 | 161 | 49 | 2 | 1 | 159 | 161 | 319 | 1.35e-70 | 216 |
| MS.gene79445.t1 | MTR_7g112970 | 66.038 | 159 | 51 | 2 | 1 | 159 | 161 | 316 | 2.43e-70 | 215 |
| MS.gene79445.t1 | MTR_7g112910 | 63.750 | 160 | 25 | 3 | 1 | 159 | 68 | 195 | 9.09e-61 | 186 |
| MS.gene79445.t1 | MTR_7g108825 | 58.228 | 158 | 65 | 1 | 2 | 159 | 161 | 317 | 1.49e-59 | 187 |
| MS.gene79445.t1 | MTR_4g120240 | 55.975 | 159 | 69 | 1 | 1 | 159 | 198 | 355 | 5.29e-58 | 184 |
| MS.gene79445.t1 | MTR_7g112615 | 87.179 | 78 | 10 | 0 | 1 | 78 | 161 | 238 | 3.91e-45 | 148 |
| MS.gene79445.t1 | MTR_7g113050 | 87.179 | 78 | 10 | 0 | 1 | 78 | 161 | 238 | 5.42e-45 | 147 |
| MS.gene79445.t1 | MTR_7g113010 | 60.656 | 122 | 44 | 2 | 2 | 121 | 162 | 281 | 1.16e-43 | 152 |
| MS.gene79445.t1 | MTR_8g076680 | 41.772 | 158 | 89 | 2 | 2 | 159 | 163 | 317 | 2.06e-33 | 120 |
| MS.gene79445.t1 | MTR_7g108825 | 51.948 | 77 | 36 | 1 | 2 | 78 | 161 | 236 | 7.72e-18 | 77.8 |
| MS.gene79445.t1 | MTR_2g090240 | 36.585 | 123 | 70 | 4 | 37 | 158 | 195 | 310 | 1.13e-17 | 78.2 |
| MS.gene79445.t1 | MTR_2g090245 | 31.875 | 160 | 99 | 5 | 2 | 158 | 158 | 310 | 1.43e-16 | 75.1 |
| MS.gene79445.t1 | MTR_4g089060 | 36.066 | 122 | 70 | 4 | 38 | 158 | 195 | 309 | 9.33e-14 | 67.4 |
| MS.gene79445.t1 | MTR_4g089060 | 36.066 | 122 | 70 | 4 | 38 | 158 | 222 | 336 | 1.24e-13 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79445.t1 | AT3G05600 | 50.932 | 161 | 77 | 2 | 1 | 159 | 164 | 324 | 1.26e-54 | 175 |
| MS.gene79445.t1 | AT4G02340 | 52.830 | 159 | 73 | 2 | 1 | 159 | 160 | 316 | 5.28e-53 | 171 |
| MS.gene79445.t1 | AT4G15960 | 49.057 | 159 | 80 | 1 | 1 | 159 | 217 | 374 | 2.13e-47 | 157 |
| MS.gene79445.t1 | AT2G26740 | 57.862 | 159 | 66 | 1 | 1 | 159 | 164 | 321 | 3.33e-47 | 155 |
| MS.gene79445.t1 | AT2G26750 | 55.975 | 159 | 69 | 1 | 1 | 159 | 163 | 320 | 2.25e-45 | 151 |
| MS.gene79445.t1 | AT4G15955 | 52.500 | 120 | 57 | 0 | 40 | 159 | 185 | 304 | 1.47e-38 | 133 |
| MS.gene79445.t1 | AT3G51000 | 39.521 | 167 | 82 | 4 | 2 | 159 | 163 | 319 | 3.02e-32 | 117 |
| MS.gene79445.t1 | AT3G05600 | 46.250 | 80 | 41 | 2 | 1 | 78 | 164 | 243 | 6.90e-17 | 75.1 |
Find 0 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTATTGTCAACATATATTTA+TGG | - | 21092:3828-3847 | MS.gene79445:intergenic | 20.0% |
| !!! | TATATGTTGACAATACTTTT+TGG | + | 21092:3832-3851 | MS.gene79445:intron | 20.0% |
| ! | AATATAACATGTATACGTGT+CGG | - | 21092:3946-3965 | MS.gene79445:intergenic | 25.0% |
| ! | ATTACTTGTATTGCAGAAAT+TGG | + | 21092:3998-4017 | MS.gene79445:intron | 25.0% |
| ! | TTACTTGTATTGCAGAAATT+GGG | + | 21092:3999-4018 | MS.gene79445:intron | 25.0% |
| AACTTGAAGGAATATATCCA+TGG | + | 21092:4108-4127 | MS.gene79445:CDS | 30.0% | |
| ATTTGGAGACATAATAAGCA+AGG | - | 21092:3716-3735 | MS.gene79445:intergenic | 30.0% | |
| ! | ATATTCCTTCAAGTTTAGTG+AGG | - | 21092:4103-4122 | MS.gene79445:intergenic | 30.0% |
| ACAATCACTTCTTCCAGATT+GGG | - | 21092:4156-4175 | MS.gene79445:intergenic | 35.0% | |
| ATACACCTCACTAAACTTGA+AGG | + | 21092:4095-4114 | MS.gene79445:CDS | 35.0% | |
| ATGGAATGGAGTGAAAATCA+AGG | + | 21092:4035-4054 | MS.gene79445:CDS | 35.0% | |
| GAAGAAGTGATTGTCCAAAA+AGG | + | 21092:4162-4181 | MS.gene79445:CDS | 35.0% | |
| GTCTCCAAATTTGAGAAAAC+AGG | + | 21092:3726-3745 | MS.gene79445:CDS | 35.0% | |
| TATTTATGGAAAACAACCCG+AGG | - | 21092:3814-3833 | MS.gene79445:intergenic | 35.0% | |
| TCACCTGTAATGAATTTCAC+AGG | - | 21092:4063-4082 | MS.gene79445:intergenic | 35.0% | |
| TGAAGGAATATATCCATGGT+GGG | + | 21092:4112-4131 | MS.gene79445:CDS | 35.0% | |
| TTGAAGGAATATATCCATGG+TGG | + | 21092:4111-4130 | MS.gene79445:CDS | 35.0% | |
| ! | GAAGCCTGTTTTCTCAAATT+TGG | - | 21092:3733-3752 | MS.gene79445:intergenic | 35.0% |
| ! | TTGATTTTCACTCCATTCCA+TGG | - | 21092:4036-4055 | MS.gene79445:intergenic | 35.0% |
| ! | TTTTGTTCTTTGTGCTACCT+CGG | + | 21092:3794-3813 | MS.gene79445:intron | 35.0% |
| AAGGAATATATCCATGGTGG+GGG | + | 21092:4114-4133 | MS.gene79445:CDS | 40.0% | |
| AGTGATTGTCCAAAAAGGAG+TGG | + | 21092:4167-4186 | MS.gene79445:CDS | 40.0% | |
| ATATTTCCCAAGGGAGAGTA+TGG | + | 21092:3633-3652 | MS.gene79445:CDS | 40.0% | |
| GAAGGAATATATCCATGGTG+GGG | + | 21092:4113-4132 | MS.gene79445:CDS | 40.0% | |
| GACAATCACTTCTTCCAGAT+TGG | - | 21092:4157-4176 | MS.gene79445:intergenic | 40.0% | |
| GTGCCTGTGAAATTCATTAC+AGG | + | 21092:4057-4076 | MS.gene79445:CDS | 40.0% | |
| TAAAAAATGCCCTCACAGCT+CGG | + | 21092:3595-3614 | MS.gene79445:CDS | 40.0% | |
| TACTCTCCCTTGGGAAATAT+TGG | - | 21092:3633-3652 | MS.gene79445:intergenic | 40.0% | |
| TCATCTTCTGTAAGCCAAGA+GGG | - | 21092:3693-3712 | MS.gene79445:intergenic | 40.0% | |
| TTTGAGAAAACAGGCTTCAC+TGG | + | 21092:3735-3754 | MS.gene79445:CDS | 40.0% | |
| TTTGTTCTTTGTGCTACCTC+GGG | + | 21092:3795-3814 | MS.gene79445:intron | 40.0% | |
| CAACAATCAAGAAGCAGCAG+AGG | + | 21092:4197-4216 | MS.gene79445:CDS | 45.0% | |
| GGTCCTCCAATATTTCCCAA+GGG | + | 21092:3624-3643 | MS.gene79445:CDS | 45.0% | |
| GTCATCTTCTGTAAGCCAAG+AGG | - | 21092:3694-3713 | MS.gene79445:intergenic | 45.0% | |
| TCTCCCTTGGGAAATATTGG+AGG | - | 21092:3630-3649 | MS.gene79445:intergenic | 45.0% | |
| TGGTCCTCCAATATTTCCCA+AGG | + | 21092:3623-3642 | MS.gene79445:CDS | 45.0% | |
| TTCTGTAAGCCAAGAGGGTA+AGG | - | 21092:3688-3707 | MS.gene79445:intergenic | 45.0% | |
| TTGCAGAAATTGGGAGCTGA+TGG | + | 21092:4008-4027 | MS.gene79445:intron | 45.0% | |
| CAAGGAAGATGTGCCCAATC+TGG | + | 21092:4140-4159 | MS.gene79445:CDS | 50.0% | |
| CCAGTTCCATACTCTCCCTT+GGG | - | 21092:3642-3661 | MS.gene79445:intergenic | 50.0% | |
| GAAGCTGAGATGGCTGAAGT+TGG | + | 21092:3558-3577 | MS.gene79445:CDS | 50.0% | |
| GAGAAAACAGGCTTCACTGG+AGG | + | 21092:3738-3757 | MS.gene79445:CDS | 50.0% | |
| ! | ACCAGTTTTCCGAGCTGTGA+GGG | - | 21092:3607-3626 | MS.gene79445:intergenic | 50.0% |
| ! | CCAAGGGAGAGTATGGAACT+GGG | + | 21092:3640-3659 | MS.gene79445:CDS | 50.0% |
| ! | TATCCATGGTGGGGGATTCA+AGG | + | 21092:4122-4141 | MS.gene79445:CDS | 50.0% |
| ! | TGTAGTGCGCCACTCCTTTT+TGG | - | 21092:4179-4198 | MS.gene79445:intergenic | 50.0% |
| !! | GGTAAGGTGTCAGGAGTATC+CGG | - | 21092:3672-3691 | MS.gene79445:intergenic | 50.0% |
| !! | GTAAGGTGTCAGGAGTATCC+GGG | - | 21092:3671-3690 | MS.gene79445:intergenic | 50.0% |
| ATTGGGAGCTGATGGCACCA+TGG | + | 21092:4016-4035 | MS.gene79445:CDS | 55.0% | |
| CCCAGTTCCATACTCTCCCT+TGG | - | 21092:3643-3662 | MS.gene79445:intergenic | 55.0% | |
| CTCCTGACACCTTACCCTCT+TGG | + | 21092:3676-3695 | MS.gene79445:CDS | 55.0% | |
| CTTCCTTGAATCCCCCACCA+TGG | - | 21092:4128-4147 | MS.gene79445:intergenic | 55.0% | |
| GAGCTGATGGCACCATGGAA+TGG | + | 21092:4021-4040 | MS.gene79445:CDS | 55.0% | |
| GTATGGAACTGGGTTCGACC+CGG | + | 21092:3650-3669 | MS.gene79445:CDS | 55.0% | |
| ! | AGCCAAGAGGGTAAGGTGTC+AGG | - | 21092:3681-3700 | MS.gene79445:intergenic | 55.0% |
| ! | CCCAAGGGAGAGTATGGAAC+TGG | + | 21092:3639-3658 | MS.gene79445:CDS | 55.0% |
| ! | GACCAGTTTTCCGAGCTGTG+AGG | - | 21092:3608-3627 | MS.gene79445:intergenic | 55.0% |
| GCCCTCACAGCTCGGAAAAC+TGG | + | 21092:3603-3622 | MS.gene79445:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 21092 | gene | 3555 | 4260 | 3555 | ID=MS.gene79445 |
| 21092 | mRNA | 3555 | 4260 | 3555 | ID=MS.gene79445.t1;Parent=MS.gene79445 |
| 21092 | exon | 3555 | 3787 | 3555 | ID=MS.gene79445.t1.exon1;Parent=MS.gene79445.t1 |
| 21092 | CDS | 3555 | 3787 | 3555 | ID=cds.MS.gene79445.t1;Parent=MS.gene79445.t1 |
| 21092 | exon | 4014 | 4260 | 4014 | ID=MS.gene79445.t1.exon2;Parent=MS.gene79445.t1 |
| 21092 | CDS | 4014 | 4260 | 4014 | ID=cds.MS.gene79445.t1;Parent=MS.gene79445.t1 |
| Gene Sequence |
| Protein sequence |
