AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80068


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80068.t1 MTR_1g104630 79.899 199 32 3 1 197 1 193 1.65e-109 311
MS.gene80068.t1 MTR_5g006130 70.833 216 37 3 1 197 1 209 1.18e-102 295
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80068.t1 AT1G03130 75.145 173 30 2 38 197 32 204 2.77e-88 258
MS.gene80068.t1 AT4G02770 73.864 176 30 2 38 197 33 208 5.39e-87 255

Find 74 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACTTGGGCTTTGCGTAAA+AGG 0.172350 1.1:+74895272 None:intergenic
CGACCCGGGTTCACTTTCTC+TGG 0.253150 1.1:+74895019 None:intergenic
AAGGGGTTGGTCAGAACTTT+AGG 0.293589 1.1:-74894994 MS.gene80068:CDS
ATTTCAAAGATTTGTTCTTT+TGG 0.302487 1.1:+74895220 None:intergenic
CCTTGCTAACTTGAGAAGAT+TGG 0.306141 1.1:+74895162 None:intergenic
GGTCAGAACTTTAGGTCTAT+TGG 0.344103 1.1:-74894986 MS.gene80068:CDS
AGCCTAAGACTGAGAGGGTT+TGG 0.354319 1.1:+74895421 None:intergenic
AGAGATACGGTCATTAGGTT+TGG 0.376453 1.1:+74895483 None:intergenic
CTCTTCACCCCTCCTCTCTC+TGG 0.385923 1.1:-74895508 MS.gene80068:CDS
AAAGAACAATGTTTGGCTCT+TGG 0.389124 1.1:-74895139 MS.gene80068:CDS
GGTACAGAGATACGGTCATT+AGG 0.394276 1.1:+74895478 None:intergenic
AGCAAGGAAAGAACAATGTT+TGG 0.394375 1.1:-74895146 MS.gene80068:CDS
CTTGCTAACTTGAGAAGATT+GGG 0.409133 1.1:+74895163 None:intergenic
TGGGAAACACTCTATAGAAT+TGG 0.417334 1.1:+74895083 None:intergenic
CCACTGAACTTAACCTCTAT+AGG 0.417689 1.1:+74894950 None:intergenic
GAGATACGGTCATTAGGTTT+GGG 0.426468 1.1:+74895484 None:intergenic
TCTGGGTACACACCATCCTT+AGG 0.434810 1.1:+74895037 None:intergenic
TGACCAACCCCTTGGCGACC+CGG 0.439253 1.1:+74895004 None:intergenic
TCTATAGAGTGTTTCCCAAT+GGG 0.441504 1.1:-74895078 MS.gene80068:CDS
AGAGAGAGGAGGGGTGAAGA+GGG 0.442080 1.1:+74895510 None:intergenic
ATCCAAACCCTCTCAGTCTT+AGG 0.461548 1.1:-74895423 MS.gene80068:CDS
AAGTATTGAACTTCCCCATT+GGG 0.462594 1.1:+74895064 None:intergenic
TACATAAAATTCCTCAACTT+GGG 0.465412 1.1:+74895258 None:intergenic
TAAGAATGTTAGTCCTATAG+AGG 0.473752 1.1:-74894963 MS.gene80068:CDS
GTTTGGGACCAGAGAGAGGA+GGG 0.477785 1.1:+74895500 None:intergenic
CAGAGAGAGGAGGGGTGAAG+AGG 0.479426 1.1:+74895509 None:intergenic
CAAATCTTTGAAATGCCTAC+TGG 0.482126 1.1:-74895211 MS.gene80068:CDS
ATGTTTGTGTCTTACTCAGG+TGG 0.482467 1.1:+74895328 None:intergenic
GACCCGGGTTCACTTTCTCT+GGG 0.486087 1.1:+74895020 None:intergenic
GAGAGGAGGGGTGAAGAGGG+AGG 0.499448 1.1:+74895513 None:intergenic
AAAGTGAACCCGGGTCGCCA+AGG 0.500800 1.1:-74895013 MS.gene80068:CDS
TTACATAAAATTCCTCAACT+TGG 0.508639 1.1:+74895257 None:intergenic
TCAGGTGGGGTGAAGCCAAT+TGG 0.510396 1.1:+74895343 None:intergenic
GAAGCAACAGAGGCACCAAT+TGG 0.510520 1.1:-74895358 MS.gene80068:CDS
AACCCGGGTCGCCAAGGGGT+TGG 0.516971 1.1:-74895007 MS.gene80068:CDS
GACCAACCCCTTGGCGACCC+GGG 0.524362 1.1:+74895005 None:intergenic
AATGTGGGTGATTGTTTCCA+TGG 0.524862 1.1:+74895457 None:intergenic
TTCTATAGAGTGTTTCCCAA+TGG 0.534519 1.1:-74895079 MS.gene80068:CDS
CAAGTATTGAACTTCCCCAT+TGG 0.539423 1.1:+74895063 None:intergenic
TGTTTGTGTCTTACTCAGGT+GGG 0.540009 1.1:+74895329 None:intergenic
TGGATTGGTGAAGGACAATG+TGG 0.545639 1.1:+74895441 None:intergenic
TGGCTCTTGGAACTACATTG+AGG 0.549525 1.1:-74895126 MS.gene80068:CDS
AAGTGAACCCGGGTCGCCAA+GGG 0.549528 1.1:-74895012 MS.gene80068:CDS
GTTTCCATGGTACAGAGATA+CGG 0.551001 1.1:+74895470 None:intergenic
GGTTTGGGACCAGAGAGAGG+AGG 0.559759 1.1:+74895499 None:intergenic
ATTGGGACCTTCCCTCATGA+TGG 0.564788 1.1:+74895180 None:intergenic
GTACCCAGAGAAAGTGAACC+CGG 0.564935 1.1:-74895023 MS.gene80068:CDS
GAGAGGGTTTGGATTGGTGA+AGG 0.565450 1.1:+74895432 None:intergenic
CAAAACAACAACTGTTACTG+AGG 0.567600 1.1:-74895380 MS.gene80068:CDS
TAAAGTTCTGACCAACCCCT+TGG 0.570035 1.1:+74894996 None:intergenic
AGTTCAATACTTGCATCCTA+AGG 0.578655 1.1:-74895053 MS.gene80068:CDS
GTTTGTGTCTTACTCAGGTG+GGG 0.582440 1.1:+74895330 None:intergenic
CCAATCTTCTCAAGTTAGCA+AGG 0.583877 1.1:-74895162 MS.gene80068:CDS
CAATACTTGCATCCTAAGGA+TGG 0.585635 1.1:-74895049 MS.gene80068:CDS
AAGACTGAGAGGGTTTGGAT+TGG 0.585730 1.1:+74895426 None:intergenic
CTGGTGGTGCAGCCATCATG+AGG 0.591206 1.1:-74895192 MS.gene80068:CDS
GAGGGAGGTTTGAGTTGCCG+TGG 0.592804 1.1:+74895528 None:intergenic
CCTATAGAGGTTAAGTTCAG+TGG 0.595489 1.1:-74894950 MS.gene80068:CDS
AAACCACCTCACAATGGCCA+CGG 0.601200 1.1:-74895545 None:intergenic
TTAGGTTTGGGACCAGAGAG+AGG 0.606251 1.1:+74895496 None:intergenic
TTTGGGACCAGAGAGAGGAG+GGG 0.614499 1.1:+74895501 None:intergenic
GGTGCAGCCATCATGAGGGA+AGG 0.635464 1.1:-74895187 MS.gene80068:CDS
TTGTGAGCCTAAGACTGAGA+GGG 0.636675 1.1:+74895416 None:intergenic
ATGATGGCTGCACCACCAGT+AGG 0.647307 1.1:+74895196 None:intergenic
TTTACGCAAAGCCCAAGTTG+AGG 0.649694 1.1:-74895269 MS.gene80068:CDS
CTATAGAGTGTTTCCCAATG+GGG 0.656977 1.1:-74895077 MS.gene80068:CDS
AGTGAACCCGGGTCGCCAAG+GGG 0.658596 1.1:-74895011 MS.gene80068:CDS
ATCTTTGAAATGCCTACTGG+TGG 0.664160 1.1:-74895208 MS.gene80068:CDS
TGTTACTGAGGAAGCAACAG+AGG 0.671184 1.1:-74895368 MS.gene80068:CDS
TGGTGGTGCAGCCATCATGA+GGG 0.675901 1.1:-74895191 MS.gene80068:CDS
ATTGTGAGCCTAAGACTGAG+AGG 0.683773 1.1:+74895415 None:intergenic
TACCCAGAGAAAGTGAACCC+GGG 0.686978 1.1:-74895022 MS.gene80068:CDS
ATGACCGTATCTCTGTACCA+TGG 0.695290 1.1:-74895474 MS.gene80068:CDS
GGATTGGTGAAGGACAATGT+GGG 0.717553 1.1:+74895442 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTCAAAGATTTGTTCTTT+TGG + chr1.1:74895243-74895262 None:intergenic 20.0%
! TACATAAAATTCCTCAACTT+GGG + chr1.1:74895205-74895224 None:intergenic 25.0%
! TTACATAAAATTCCTCAACT+TGG + chr1.1:74895206-74895225 None:intergenic 25.0%
!! AGGAATTTTATGTAATCACA+TGG - chr1.1:74895211-74895230 MS.gene80068:CDS 25.0%
!! GGAATTTTATGTAATCACAT+GGG - chr1.1:74895212-74895231 MS.gene80068:CDS 25.0%
TAAGAATGTTAGTCCTATAG+AGG - chr1.1:74895497-74895516 MS.gene80068:CDS 30.0%
AGCAAGGAAAGAACAATGTT+TGG - chr1.1:74895314-74895333 MS.gene80068:intron 35.0%
AGTTCAATACTTGCATCCTA+AGG - chr1.1:74895407-74895426 MS.gene80068:CDS 35.0%
CAAAACAACAACTGTTACTG+AGG - chr1.1:74895080-74895099 MS.gene80068:CDS 35.0%
CTTGCTAACTTGAGAAGATT+GGG + chr1.1:74895300-74895319 None:intergenic 35.0%
TGGGAAACACTCTATAGAAT+TGG + chr1.1:74895380-74895399 None:intergenic 35.0%
! TCTATAGAGTGTTTCCCAAT+GGG - chr1.1:74895382-74895401 MS.gene80068:CDS 35.0%
! TTCTATAGAGTGTTTCCCAA+TGG - chr1.1:74895381-74895400 MS.gene80068:CDS 35.0%
!! AAAGAACAATGTTTGGCTCT+TGG - chr1.1:74895321-74895340 MS.gene80068:intron 35.0%
!! AAGTATTGAACTTCCCCATT+GGG + chr1.1:74895399-74895418 None:intergenic 35.0%
!! CAAATCTTTGAAATGCCTAC+TGG - chr1.1:74895249-74895268 MS.gene80068:CDS 35.0%
AATGTGGGTGATTGTTTCCA+TGG + chr1.1:74895006-74895025 None:intergenic 40.0%
AGAGATACGGTCATTAGGTT+TGG + chr1.1:74894980-74894999 None:intergenic 40.0%
AGTACTGCCACTGAAAATTG+GGG + chr1.1:74895163-74895182 None:intergenic 40.0%
ATGTTTGTGTCTTACTCAGG+TGG + chr1.1:74895135-74895154 None:intergenic 40.0%
CAATACTTGCATCCTAAGGA+TGG - chr1.1:74895411-74895430 MS.gene80068:CDS 40.0%
CAGTACTGCCACTGAAAATT+GGG + chr1.1:74895164-74895183 None:intergenic 40.0%
CCAATCTTCTCAAGTTAGCA+AGG - chr1.1:74895298-74895317 MS.gene80068:intron 40.0%
CCACTGAACTTAACCTCTAT+AGG + chr1.1:74895513-74895532 None:intergenic 40.0%
CCTATAGAGGTTAAGTTCAG+TGG - chr1.1:74895510-74895529 MS.gene80068:CDS 40.0%
CCTTGCTAACTTGAGAAGAT+TGG + chr1.1:74895301-74895320 None:intergenic 40.0%
GAGATACGGTCATTAGGTTT+GGG + chr1.1:74894979-74894998 None:intergenic 40.0%
GGAATGTTTGTGTCTTACTC+AGG + chr1.1:74895138-74895157 None:intergenic 40.0%
GGTCAGAACTTTAGGTCTAT+TGG - chr1.1:74895474-74895493 MS.gene80068:CDS 40.0%
GTTTCCATGGTACAGAGATA+CGG + chr1.1:74894993-74895012 None:intergenic 40.0%
TGTTTGTGTCTTACTCAGGT+GGG + chr1.1:74895134-74895153 None:intergenic 40.0%
! CTATAGAGTGTTTCCCAATG+GGG - chr1.1:74895383-74895402 MS.gene80068:CDS 40.0%
!! ATCTTTGAAATGCCTACTGG+TGG - chr1.1:74895252-74895271 MS.gene80068:CDS 40.0%
!! CAAGTATTGAACTTCCCCAT+TGG + chr1.1:74895400-74895419 None:intergenic 40.0%
AAGACTGAGAGGGTTTGGAT+TGG + chr1.1:74895037-74895056 None:intergenic 45.0%
ATCCAAACCCTCTCAGTCTT+AGG - chr1.1:74895037-74895056 MS.gene80068:CDS 45.0%
ATGACCGTATCTCTGTACCA+TGG - chr1.1:74894986-74895005 MS.gene80068:CDS 45.0%
ATTGTGAGCCTAAGACTGAG+AGG + chr1.1:74895048-74895067 None:intergenic 45.0%
CAACTTGGGCTTTGCGTAAA+AGG + chr1.1:74895191-74895210 None:intergenic 45.0%
CCAGTACTGCCACTGAAAAT+TGG + chr1.1:74895165-74895184 None:intergenic 45.0%
GGTACAGAGATACGGTCATT+AGG + chr1.1:74894985-74895004 None:intergenic 45.0%
GTTTGTGTCTTACTCAGGTG+GGG + chr1.1:74895133-74895152 None:intergenic 45.0%
TGGCTCTTGGAACTACATTG+AGG - chr1.1:74895334-74895353 MS.gene80068:intron 45.0%
TGTTACTGAGGAAGCAACAG+AGG - chr1.1:74895092-74895111 MS.gene80068:CDS 45.0%
TTGTGAGCCTAAGACTGAGA+GGG + chr1.1:74895047-74895066 None:intergenic 45.0%
TTTACGCAAAGCCCAAGTTG+AGG - chr1.1:74895191-74895210 MS.gene80068:CDS 45.0%
! ATTCCCTCCCCAATTTTCAG+TGG - chr1.1:74895153-74895172 MS.gene80068:CDS 45.0%
! CCAATTTTCAGTGGCAGTAC+TGG - chr1.1:74895162-74895181 MS.gene80068:CDS 45.0%
! GGATTGGTGAAGGACAATGT+GGG + chr1.1:74895021-74895040 None:intergenic 45.0%
! TAAAGTTCTGACCAACCCCT+TGG + chr1.1:74895467-74895486 None:intergenic 45.0%
!! AAGGGGTTGGTCAGAACTTT+AGG - chr1.1:74895466-74895485 MS.gene80068:CDS 45.0%
!! ATTTTCAGTGGCAGTACTGG+AGG - chr1.1:74895165-74895184 MS.gene80068:CDS 45.0%
!! TGGATTGGTGAAGGACAATG+TGG + chr1.1:74895022-74895041 None:intergenic 45.0%
ACTGCCACTGAAAATTGGGG+AGG + chr1.1:74895160-74895179 None:intergenic 50.0%
AGCCTAAGACTGAGAGGGTT+TGG + chr1.1:74895042-74895061 None:intergenic 50.0%
ATTGGGACCTTCCCTCATGA+TGG + chr1.1:74895283-74895302 None:intergenic 50.0%
CTGCCACTGAAAATTGGGGA+GGG + chr1.1:74895159-74895178 None:intergenic 50.0%
GTACCCAGAGAAAGTGAACC+CGG - chr1.1:74895437-74895456 MS.gene80068:CDS 50.0%
TACCCAGAGAAAGTGAACCC+GGG - chr1.1:74895438-74895457 MS.gene80068:CDS 50.0%
TCTGGGTACACACCATCCTT+AGG + chr1.1:74895426-74895445 None:intergenic 50.0%
! TTAGGTTTGGGACCAGAGAG+AGG + chr1.1:74894967-74894986 None:intergenic 50.0%
!! GAAGCAACAGAGGCACCAAT+TGG - chr1.1:74895102-74895121 MS.gene80068:CDS 50.0%
!! GAGAGGGTTTGGATTGGTGA+AGG + chr1.1:74895031-74895050 None:intergenic 50.0%
AGAGAGAGGAGGGGTGAAGA+GGG + chr1.1:74894953-74894972 None:intergenic 55.0%
ATGATGGCTGCACCACCAGT+AGG + chr1.1:74895267-74895286 None:intergenic 55.0%
GACCCGGGTTCACTTTCTCT+GGG + chr1.1:74895443-74895462 None:intergenic 55.0%
TCAGGTGGGGTGAAGCCAAT+TGG + chr1.1:74895120-74895139 None:intergenic 55.0%
! GTTTGGGACCAGAGAGAGGA+GGG + chr1.1:74894963-74894982 None:intergenic 55.0%
! TGGTGGTGCAGCCATCATGA+GGG - chr1.1:74895269-74895288 MS.gene80068:CDS 55.0%
! TTTGGGACCAGAGAGAGGAG+GGG + chr1.1:74894962-74894981 None:intergenic 55.0%
AAAGTGAACCCGGGTCGCCA+AGG - chr1.1:74895447-74895466 MS.gene80068:CDS 60.0%
AAGTGAACCCGGGTCGCCAA+GGG - chr1.1:74895448-74895467 MS.gene80068:CDS 60.0%
CAGAGAGAGGAGGGGTGAAG+AGG + chr1.1:74894954-74894973 None:intergenic 60.0%
CGACCCGGGTTCACTTTCTC+TGG + chr1.1:74895444-74895463 None:intergenic 60.0%
CTCTTCACCCCTCCTCTCTC+TGG - chr1.1:74894952-74894971 MS.gene80068:CDS 60.0%
GGTGCAGCCATCATGAGGGA+AGG - chr1.1:74895273-74895292 MS.gene80068:CDS 60.0%
! CTGGTGGTGCAGCCATCATG+AGG - chr1.1:74895268-74895287 MS.gene80068:CDS 60.0%
! GGTTTGGGACCAGAGAGAGG+AGG + chr1.1:74894964-74894983 None:intergenic 60.0%
!! GAGGGAGGTTTGAGTTGCCG+TGG + chr1.1:74894935-74894954 None:intergenic 60.0%
AGTGAACCCGGGTCGCCAAG+GGG - chr1.1:74895449-74895468 MS.gene80068:CDS 65.0%
GAGAGGAGGGGTGAAGAGGG+AGG + chr1.1:74894950-74894969 None:intergenic 65.0%
TGACCAACCCCTTGGCGACC+CGG + chr1.1:74895459-74895478 None:intergenic 65.0%
AACCCGGGTCGCCAAGGGGT+TGG - chr1.1:74895453-74895472 MS.gene80068:CDS 70.0%
GACCAACCCCTTGGCGACCC+GGG + chr1.1:74895458-74895477 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 74894928 74895554 74894928 ID=MS.gene80068
chr1.1 mRNA 74894928 74895554 74894928 ID=MS.gene80068.t1;Parent=MS.gene80068
chr1.1 exon 74895343 74895554 74895343 ID=MS.gene80068.t1.exon1;Parent=MS.gene80068.t1
chr1.1 CDS 74895343 74895554 74895343 ID=cds.MS.gene80068.t1;Parent=MS.gene80068.t1
chr1.1 exon 74894928 74895309 74894928 ID=MS.gene80068.t1.exon2;Parent=MS.gene80068.t1
chr1.1 CDS 74894928 74895309 74894928 ID=cds.MS.gene80068.t1;Parent=MS.gene80068.t1
Gene Sequence

>MS.gene80068

ATGGCCACGGCAACTCAAACCTCCCTCTTCACCCCTCCTCTCTCTGGTCCCAAACCTAATGACCGTATCTCTGTACCATGGAAACAATCACCCACATTGTCCTTCACCAATCCAAACCCTCTCAGTCTTAGGCTCACAATTTCAAGAAACATCAAAACAACAACTGTTACTGAGGAAGCAACAGAGGCACCAATTGGCTTCACCCCACCTGAGTAAGACACAAACATTCCCTCCCCAATTTTCAGTGGCAGTACTGGAGGCCTTTTACGCAAAGCCCAAGTTGAGGAATTTTATGTAATCACATGGGATTCACCAAAAGAACAAATCTTTGAAATGCCTACTGGTGGTGCAGCCATCATGAGGGAAGGTCCCAATCTTCTCAAGTTAGCAAGGAAAGAACAATGTTTGGCTCTTGGAACTACATTGAGGTCAAAGTACAAGATCAAGTACCAATTCTATAGAGTGTTTCCCAATGGGGAAGTTCAATACTTGCATCCTAAGGATGGTGTGTACCCAGAGAAAGTGAACCCGGGTCGCCAAGGGGTTGGTCAGAACTTTAGGTCTATTGGTAAGAATGTTAGTCCTATAGAGGTTAAGTTCAGTGGCAAGCAAGTGCATGAATTGTGA

Protein sequence

>MS.gene80068.t1

MATATQTSLFTPPLSGPKPNDRISVPWKQSPTLSFTNPNPLSLRLTISRNIKTTTVTEEATEAPIGFTPPDGSTGGLLRKAQVEEFYVITWDSPKEQIFEMPTGGAAIMREGPNLLKLARKEQCLALGTTLRSKYKIKYQFYRVFPNGEVQYLHPKDGVYPEKVNPGRQGVGQNFRSIGKNVSPIEVKFSGKQVHEL