Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82983.t1 | AFK36855.1 | 96 | 75 | 2 | 1 | 1 | 75 | 24 | 97 | 8.20E-29 | 136.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82983.t1 | Q9ZQ80 | 54.4 | 68 | 27 | 1 | 1 | 68 | 69 | 132 | 5.1e-11 | 68.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82983.t1 | G7JGC6 | 96.0 | 75 | 2 | 1 | 1 | 75 | 24 | 97 | 5.9e-29 | 136.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049293 | MS.gene82983 | -0.823807 | 1.14E-53 | -1.69E-46 |
| MS.gene049322 | MS.gene82983 | 0.816773 | 4.65E-52 | -1.69E-46 |
| MS.gene049438 | MS.gene82983 | 0.806722 | 7.17E-50 | -1.69E-46 |
| MS.gene04991 | MS.gene82983 | -0.818743 | 1.67E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene82983 | -0.811014 | 8.65E-51 | -1.69E-46 |
| MS.gene051245 | MS.gene82983 | 0.813876 | 2.05E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene82983 | 0.834093 | 3.66E-56 | -1.69E-46 |
| MS.gene052302 | MS.gene82983 | 0.848217 | 7.05E-60 | -1.69E-46 |
| MS.gene052309 | MS.gene82983 | -0.860511 | 1.96E-63 | -1.69E-46 |
| MS.gene052376 | MS.gene82983 | 0.835148 | 1.99E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene82983 | 0.807137 | 5.85E-50 | -1.69E-46 |
| MS.gene054946 | MS.gene82983 | -0.810721 | 1.00E-50 | -1.69E-46 |
| MS.gene055174 | MS.gene82983 | 0.804037 | 2.62E-49 | -1.69E-46 |
| MS.gene057079 | MS.gene82983 | 0.824831 | 6.52E-54 | -1.69E-46 |
| MS.gene057451 | MS.gene82983 | -0.817893 | 2.60E-52 | -1.69E-46 |
| MS.gene057452 | MS.gene82983 | -0.824818 | 6.57E-54 | -1.69E-46 |
| MS.gene057999 | MS.gene82983 | 0.814317 | 1.64E-51 | -1.69E-46 |
| MS.gene058074 | MS.gene82983 | 0.81527 | 1.01E-51 | -1.69E-46 |
| MS.gene058182 | MS.gene82983 | 0.806919 | 6.51E-50 | -1.69E-46 |
| MS.gene058201 | MS.gene82983 | 0.800579 | 1.35E-48 | -1.69E-46 |
| MS.gene058238 | MS.gene82983 | 0.83049 | 2.85E-55 | -1.69E-46 |
| MS.gene058661 | MS.gene82983 | -0.832294 | 1.03E-55 | -1.69E-46 |
| MS.gene059872 | MS.gene82983 | -0.86681 | 2.17E-65 | -1.69E-46 |
| MS.gene060297 | MS.gene82983 | 0.865038 | 7.89E-65 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82983.t1 | MTR_4g082830 | 95.370 | 108 | 4 | 1 | 1 | 108 | 24 | 130 | 1.35e-65 | 194 |
| MS.gene82983.t1 | MTR_4g082860 | 64.286 | 98 | 23 | 3 | 1 | 97 | 16 | 102 | 6.51e-31 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82983.t1 | AT2G03440 | 55.882 | 68 | 26 | 1 | 1 | 68 | 69 | 132 | 5.34e-12 | 59.7 |
Find 38 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGCAGCACAATCAAGTTT+AGG | 0.292106 | 4.2:-26901329 | MS.gene82983:CDS |
| AGTTGGTCAGTATGCTAAAT+TGG | 0.398975 | 4.2:-26901231 | MS.gene82983:CDS |
| AGGCAGCACAATCAAGTTTA+GGG | 0.405707 | 4.2:-26901328 | MS.gene82983:CDS |
| TGCTCCACCTTCTTCTAAAC+CGG | 0.409063 | 4.2:-26901120 | MS.gene82983:CDS |
| GTGGCCGCATCATGACCTTT+AGG | 0.409931 | 4.2:+26901146 | None:intergenic |
| TTGGATGATCAGAAAGGGTT+AGG | 0.437630 | 4.2:-26901212 | MS.gene82983:CDS |
| GGTGGAGGAGGTGGACATCA+TGG | 0.442324 | 4.2:-26901062 | MS.gene82983:CDS |
| TCATCACCTTTGTGATGATC+CGG | 0.443483 | 4.2:+26901101 | None:intergenic |
| TGATGATCCGGTTTAGAAGA+AGG | 0.446935 | 4.2:+26901113 | None:intergenic |
| GCGAAGGTGGCGGAGGCTGC+AGG | 0.456404 | 4.2:-26901269 | MS.gene82983:CDS |
| GTTAGGACAGTATGTTGATA+AGG | 0.464040 | 4.2:-26901195 | MS.gene82983:CDS |
| AAGTCTGAAGGTGGAGGAGG+TGG | 0.467957 | 4.2:-26901071 | MS.gene82983:CDS |
| TCATAATAAGCCTATGGATA+AGG | 0.491910 | 4.2:-26901291 | MS.gene82983:CDS |
| CTAAATTGGATGATCAGAAA+GGG | 0.509410 | 4.2:-26901217 | MS.gene82983:CDS |
| GCTAAATTGGATGATCAGAA+AGG | 0.518563 | 4.2:-26901218 | MS.gene82983:CDS |
| TGCAGGTCATAATAAGCCTA+TGG | 0.519120 | 4.2:-26901297 | MS.gene82983:CDS |
| CTGCATGGTTATCATCCTAA+AGG | 0.528247 | 4.2:-26901161 | MS.gene82983:CDS |
| GGAGGTGGACATCATGGGCT+AGG | 0.531113 | 4.2:-26901056 | MS.gene82983:CDS |
| GCCACCTTCGCCTTATCCAT+AGG | 0.532358 | 4.2:+26901281 | None:intergenic |
| GGAGATCTTCTTGATGCAGT+TGG | 0.535329 | 4.2:-26901248 | MS.gene82983:CDS |
| GTGGAGGAGGTGGACATCAT+GGG | 0.539472 | 4.2:-26901061 | MS.gene82983:CDS |
| GGATAAGGCGAAGGTGGCGG+AGG | 0.544131 | 4.2:-26901276 | MS.gene82983:CDS |
| TATGGATAAGGCGAAGGTGG+CGG | 0.557696 | 4.2:-26901279 | MS.gene82983:CDS |
| GATAAGTCTGAAGGTGGAGG+AGG | 0.559044 | 4.2:-26901074 | MS.gene82983:CDS |
| AGTTTAGGGAAAGGATCTGC+AGG | 0.562101 | 4.2:-26901314 | MS.gene82983:CDS |
| GCCTATGGATAAGGCGAAGG+TGG | 0.589298 | 4.2:-26901282 | MS.gene82983:CDS |
| GCGGATAAGTCTGAAGGTGG+AGG | 0.600426 | 4.2:-26901077 | MS.gene82983:CDS |
| GCACAATCAAGTTTAGGGAA+AGG | 0.600593 | 4.2:-26901323 | MS.gene82983:CDS |
| AGAAGAAGGTGGAGCAGTGG+TGG | 0.601687 | 4.2:+26901127 | None:intergenic |
| AAGGCTGCTGATTATCTGCA+TGG | 0.619240 | 4.2:-26901176 | MS.gene82983:CDS |
| TAAGCCTATGGATAAGGCGA+AGG | 0.625524 | 4.2:-26901285 | MS.gene82983:CDS |
| TTTAGAAGAAGGTGGAGCAG+TGG | 0.644489 | 4.2:+26901124 | None:intergenic |
| TCATCACAAAGGTGATGATG+CGG | 0.659363 | 4.2:-26901096 | MS.gene82983:CDS |
| GATGCGGATAAGTCTGAAGG+TGG | 0.683392 | 4.2:-26901080 | MS.gene82983:CDS |
| TCTAAACCGGATCATCACAA+AGG | 0.690243 | 4.2:-26901107 | MS.gene82983:CDS |
| TCATCCTAAAGGTCATGATG+CGG | 0.692039 | 4.2:-26901150 | MS.gene82983:CDS |
| GATGATGCGGATAAGTCTGA+AGG | 0.699420 | 4.2:-26901083 | MS.gene82983:CDS |
| TGATCCGGTTTAGAAGAAGG+TGG | 0.716579 | 4.2:+26901116 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CTAAATTGGATGATCAGAAA+GGG | - | chr4.2:26901182-26901201 | MS.gene82983:CDS | 30.0% | |
| TCATAATAAGCCTATGGATA+AGG | - | chr4.2:26901108-26901127 | MS.gene82983:CDS | 30.0% | |
| AGTTGGTCAGTATGCTAAAT+TGG | - | chr4.2:26901168-26901187 | MS.gene82983:CDS | 35.0% | |
| GCTAAATTGGATGATCAGAA+AGG | - | chr4.2:26901181-26901200 | MS.gene82983:CDS | 35.0% | |
| GTTAGGACAGTATGTTGATA+AGG | - | chr4.2:26901204-26901223 | MS.gene82983:CDS | 35.0% | |
| AGGCAGCACAATCAAGTTTA+GGG | - | chr4.2:26901071-26901090 | MS.gene82983:CDS | 40.0% | |
| CTGCATGGTTATCATCCTAA+AGG | - | chr4.2:26901238-26901257 | MS.gene82983:CDS | 40.0% | |
| GAGTGCAAAAATTGTAGCAG+AGG | - | chr4.2:26901051-26901070 | MS.gene82983:CDS | 40.0% | |
| GCACAATCAAGTTTAGGGAA+AGG | - | chr4.2:26901076-26901095 | MS.gene82983:CDS | 40.0% | |
| TCATCACCTTTGTGATGATC+CGG | + | chr4.2:26901301-26901320 | None:intergenic | 40.0% | |
| TCATCCTAAAGGTCATGATG+CGG | - | chr4.2:26901249-26901268 | MS.gene82983:CDS | 40.0% | |
| TCTAAACCGGATCATCACAA+AGG | - | chr4.2:26901292-26901311 | MS.gene82983:CDS | 40.0% | |
| TGATGATCCGGTTTAGAAGA+AGG | + | chr4.2:26901289-26901308 | None:intergenic | 40.0% | |
| TGCAGGTCATAATAAGCCTA+TGG | - | chr4.2:26901102-26901121 | MS.gene82983:CDS | 40.0% | |
| TTGGATGATCAGAAAGGGTT+AGG | - | chr4.2:26901187-26901206 | MS.gene82983:CDS | 40.0% | |
| ! | TCATCACAAAGGTGATGATG+CGG | - | chr4.2:26901303-26901322 | MS.gene82983:CDS | 40.0% |
| AGTTTAGGGAAAGGATCTGC+AGG | - | chr4.2:26901085-26901104 | MS.gene82983:CDS | 45.0% | |
| GAGGCAGCACAATCAAGTTT+AGG | - | chr4.2:26901070-26901089 | MS.gene82983:CDS | 45.0% | |
| GATGATGCGGATAAGTCTGA+AGG | - | chr4.2:26901316-26901335 | MS.gene82983:CDS | 45.0% | |
| GGAGATCTTCTTGATGCAGT+TGG | - | chr4.2:26901151-26901170 | MS.gene82983:CDS | 45.0% | |
| TAAGCCTATGGATAAGGCGA+AGG | - | chr4.2:26901114-26901133 | MS.gene82983:CDS | 45.0% | |
| TGATCCGGTTTAGAAGAAGG+TGG | + | chr4.2:26901286-26901305 | None:intergenic | 45.0% | |
| TGCTCCACCTTCTTCTAAAC+CGG | - | chr4.2:26901279-26901298 | MS.gene82983:CDS | 45.0% | |
| TTTAGAAGAAGGTGGAGCAG+TGG | + | chr4.2:26901278-26901297 | None:intergenic | 45.0% | |
| ! | AAGGCTGCTGATTATCTGCA+TGG | - | chr4.2:26901223-26901242 | MS.gene82983:CDS | 45.0% |
| GATAAGTCTGAAGGTGGAGG+AGG | - | chr4.2:26901325-26901344 | MS.gene82983:CDS | 50.0% | |
| GATGCGGATAAGTCTGAAGG+TGG | - | chr4.2:26901319-26901338 | MS.gene82983:CDS | 50.0% | |
| TATGGATAAGGCGAAGGTGG+CGG | - | chr4.2:26901120-26901139 | MS.gene82983:CDS | 50.0% | |
| AAGTCTGAAGGTGGAGGAGG+TGG | - | chr4.2:26901328-26901347 | MS.gene82983:CDS | 55.0% | |
| AGAAGAAGGTGGAGCAGTGG+TGG | + | chr4.2:26901275-26901294 | None:intergenic | 55.0% | |
| GCCACCTTCGCCTTATCCAT+AGG | + | chr4.2:26901121-26901140 | None:intergenic | 55.0% | |
| GCCTATGGATAAGGCGAAGG+TGG | - | chr4.2:26901117-26901136 | MS.gene82983:CDS | 55.0% | |
| GCGGATAAGTCTGAAGGTGG+AGG | - | chr4.2:26901322-26901341 | MS.gene82983:CDS | 55.0% | |
| GTGGAGGAGGTGGACATCAT+GGG | - | chr4.2:26901338-26901357 | MS.gene82983:CDS | 55.0% | |
| GTGGCCGCATCATGACCTTT+AGG | + | chr4.2:26901256-26901275 | None:intergenic | 55.0% | |
| GGAGGTGGACATCATGGGCT+AGG | - | chr4.2:26901343-26901362 | MS.gene82983:CDS | 60.0% | |
| GGTGGAGGAGGTGGACATCA+TGG | - | chr4.2:26901337-26901356 | MS.gene82983:CDS | 60.0% | |
| ! | GGATAAGGCGAAGGTGGCGG+AGG | - | chr4.2:26901123-26901142 | MS.gene82983:CDS | 65.0% |
| !! | GCGAAGGTGGCGGAGGCTGC+AGG | - | chr4.2:26901130-26901149 | MS.gene82983:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26901046 | 26901375 | 26901046 | ID=MS.gene82983 |
| chr4.2 | mRNA | 26901046 | 26901375 | 26901046 | ID=MS.gene82983.t1;Parent=MS.gene82983 |
| chr4.2 | exon | 26901046 | 26901375 | 26901046 | ID=MS.gene82983.t1.exon1;Parent=MS.gene82983.t1 |
| chr4.2 | CDS | 26901046 | 26901375 | 26901046 | ID=cds.MS.gene82983.t1;Parent=MS.gene82983.t1 |
| Gene Sequence |
| Protein sequence |
