Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83324.t1 | XP_003605540.1 | 96.9 | 381 | 8 | 1 | 1 | 381 | 1 | 377 | 8.80E-211 | 742.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83324.t1 | F4HYD7 | 64.1 | 368 | 127 | 1 | 14 | 381 | 14 | 376 | 1.8e-140 | 500.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83324.t1 | G7JP72 | 96.9 | 381 | 8 | 1 | 1 | 381 | 1 | 377 | 6.3e-211 | 742.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene83324 | 0.80554 | 1.27E-49 | -1.69E-46 |
| MS.gene049283 | MS.gene83324 | 0.823405 | 1.41E-53 | -1.69E-46 |
| MS.gene049499 | MS.gene83324 | 0.800525 | 1.38E-48 | -1.69E-46 |
| MS.gene049801 | MS.gene83324 | 0.804613 | 1.99E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene83324 | 0.817109 | 3.91E-52 | -1.69E-46 |
| MS.gene051332 | MS.gene83324 | 0.811588 | 6.50E-51 | -1.69E-46 |
| MS.gene052118 | MS.gene83324 | 0.813314 | 2.73E-51 | -1.69E-46 |
| MS.gene052674 | MS.gene83324 | 0.824255 | 8.91E-54 | -1.69E-46 |
| MS.gene052675 | MS.gene83324 | 0.822573 | 2.20E-53 | -1.69E-46 |
| MS.gene052677 | MS.gene83324 | 0.821928 | 3.11E-53 | -1.69E-46 |
| MS.gene054479 | MS.gene83324 | 0.833316 | 5.72E-56 | -1.69E-46 |
| MS.gene054641 | MS.gene83324 | 0.821229 | 4.51E-53 | -1.69E-46 |
| MS.gene054892 | MS.gene83324 | 0.80545 | 1.33E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene83324 | 0.807787 | 4.26E-50 | -1.69E-46 |
| MS.gene056045 | MS.gene83324 | 0.83382 | 4.28E-56 | -1.69E-46 |
| MS.gene056509 | MS.gene83324 | 0.804143 | 2.49E-49 | -1.69E-46 |
| MS.gene056831 | MS.gene83324 | 0.840122 | 1.05E-57 | -1.69E-46 |
| MS.gene057208 | MS.gene83324 | 0.80069 | 1.28E-48 | -1.69E-46 |
| MS.gene058159 | MS.gene83324 | 0.826728 | 2.31E-54 | -1.69E-46 |
| MS.gene058223 | MS.gene83324 | -0.835677 | 1.46E-56 | -1.69E-46 |
| MS.gene058429 | MS.gene83324 | 0.800413 | 1.46E-48 | -1.69E-46 |
| MS.gene059144 | MS.gene83324 | 0.800692 | 1.28E-48 | -1.69E-46 |
| MS.gene059439 | MS.gene83324 | 0.822628 | 2.14E-53 | -1.69E-46 |
| MS.gene05952 | MS.gene83324 | 0.800378 | 1.48E-48 | -1.69E-46 |
| MS.gene060527 | MS.gene83324 | 0.819191 | 1.32E-52 | -1.69E-46 |
| MS.gene060777 | MS.gene83324 | 0.966665 | 3.17E-126 | -1.69E-46 |
| MS.gene060932 | MS.gene83324 | 0.848311 | 6.65E-60 | -1.69E-46 |
| MS.gene060933 | MS.gene83324 | 0.826695 | 2.36E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene83324 | 0.81618 | 6.32E-52 | -1.69E-46 |
| MS.gene060937 | MS.gene83324 | 0.835899 | 1.28E-56 | -1.69E-46 |
| MS.gene061100 | MS.gene83324 | 0.807251 | 5.54E-50 | -1.69E-46 |
| MS.gene061160 | MS.gene83324 | 0.82092 | 5.32E-53 | -1.69E-46 |
| MS.gene06121 | MS.gene83324 | 0.82976 | 4.30E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83324.t1 | MTR_4g032800 | 96.850 | 381 | 8 | 1 | 1 | 381 | 1 | 377 | 0.0 | 766 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83324.t1 | AT1G35470 | 64.130 | 368 | 127 | 1 | 14 | 381 | 14 | 376 | 0.0 | 510 |
| MS.gene83324.t1 | AT1G35470 | 63.587 | 368 | 127 | 2 | 14 | 381 | 14 | 374 | 3.20e-177 | 501 |
| MS.gene83324.t1 | AT4G09340 | 67.463 | 335 | 107 | 1 | 47 | 381 | 21 | 353 | 1.25e-167 | 476 |
| MS.gene83324.t1 | AT4G09200 | 41.994 | 331 | 171 | 8 | 55 | 379 | 12 | 327 | 3.17e-79 | 249 |
| MS.gene83324.t1 | AT4G09310 | 41.994 | 331 | 171 | 8 | 55 | 379 | 12 | 327 | 3.17e-79 | 249 |
| MS.gene83324.t1 | AT4G09250 | 41.528 | 301 | 152 | 8 | 55 | 349 | 12 | 294 | 2.08e-70 | 224 |
Find 90 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAAGGATGCTGGTGTTAA+AGG | 0.230981 | 4.1:-68002141 | MS.gene83324:CDS |
| TTGTTTCAATGAAGCTAATC+AGG | 0.293085 | 4.1:-67994292 | MS.gene83324:intron |
| CTGGAGGAGGCTGTCAAATA+TGG | 0.316922 | 4.1:-67991788 | MS.gene83324:CDS |
| CAGGGTAAAGGGGAAGCTTT+TGG | 0.318442 | 4.1:-68000911 | MS.gene83324:CDS |
| TACGACTCAATTCTAAGAAA+TGG | 0.325466 | 4.1:+68002391 | None:intergenic |
| CTTTACCCTGTCCACGATAA+AGG | 0.331990 | 4.1:+68000924 | None:intergenic |
| AAAATTTATCGAACTAGTTC+GGG | 0.335513 | 4.1:-67993057 | MS.gene83324:intron |
| TCTCTTTGAGGATTTAGTTC+AGG | 0.348395 | 4.1:-67991732 | None:intergenic |
| GATAGCACACCTCAGCAATC+TGG | 0.361401 | 4.1:+67994222 | None:intergenic |
| CAAATTCAAAATCAAGATCT+AGG | 0.369691 | 4.1:-68002417 | MS.gene83324:CDS |
| TCAACGCATATGTTGTTTCC+TGG | 0.370298 | 4.1:+67995075 | None:intergenic |
| TTTATCGTGGACAGGGTAAA+GGG | 0.407139 | 4.1:-68000922 | MS.gene83324:CDS |
| TATACGAGTGTGAATCTTCA+TGG | 0.407792 | 4.1:-68002237 | MS.gene83324:CDS |
| ACCATTCACCTTTGACCTAA+AGG | 0.407822 | 4.1:-67999027 | MS.gene83324:intron |
| AACAGGGTACATGTCAACTT+TGG | 0.412192 | 4.1:-67999056 | MS.gene83324:intron |
| TCTCACCCATAACTGGCATT+TGG | 0.412599 | 4.1:+67998009 | None:intergenic |
| AACAGCAACAGTTGGGAAAA+GGG | 0.414213 | 4.1:+68000288 | None:intergenic |
| TGTTGCTACTGATAAGCTTT+CGG | 0.414522 | 4.1:-68002265 | MS.gene83324:CDS |
| CACAGTTTGTAAAGAAATGA+AGG | 0.414745 | 4.1:-68000314 | MS.gene83324:CDS |
| CTTTATCGTGGACAGGGTAA+AGG | 0.419684 | 4.1:-68000923 | MS.gene83324:CDS |
| GAACAGCAACAGTTGGGAAA+AGG | 0.429415 | 4.1:+68000287 | None:intergenic |
| AACTATATCACCAGTGGTAT+AGG | 0.432201 | 4.1:+68000883 | None:intergenic |
| GTACAACATACTCTTCATTC+TGG | 0.435067 | 4.1:+68000260 | None:intergenic |
| GTTAAAGGGCAGGTTTCGAT+TGG | 0.438082 | 4.1:-68002126 | MS.gene83324:CDS |
| TTAAGGATGCTGGTGTTAAA+GGG | 0.450841 | 4.1:-68002140 | MS.gene83324:CDS |
| GGGGAGATTGATGCTGCATT+TGG | 0.451426 | 4.1:-67994266 | MS.gene83324:CDS |
| ACCTTTAGGTCAAAGGTGAA+TGG | 0.451440 | 4.1:+67999026 | None:intergenic |
| TGGTTTCCTTTATCGTGGAC+AGG | 0.453833 | 4.1:-68000930 | MS.gene83324:CDS |
| TGGAATTAACTATGCTGCTC+AGG | 0.455934 | 4.1:-68000855 | MS.gene83324:CDS |
| ACAGGGTACATGTCAACTTT+GGG | 0.463817 | 4.1:-67999055 | MS.gene83324:intron |
| ATAGCACACCTCAGCAATCT+GGG | 0.466738 | 4.1:+67994223 | None:intergenic |
| AGTTGATGATGAAGAGAAAG+AGG | 0.469005 | 4.1:-68002337 | MS.gene83324:CDS |
| GTGTCACCAAATGCCAGTTA+TGG | 0.473579 | 4.1:-67998015 | MS.gene83324:CDS |
| CGTTCCTACCTGCTACACTA+TGG | 0.480483 | 4.1:-67995185 | MS.gene83324:CDS |
| CTTCATAGCCATAGTGTAGC+AGG | 0.480538 | 4.1:+67995177 | None:intergenic |
| CATTTGGTAAATTGCGTGAA+TGG | 0.481704 | 4.1:-67994250 | MS.gene83324:CDS |
| TGTCACCAAATGCCAGTTAT+GGG | 0.494644 | 4.1:-67998014 | MS.gene83324:intron |
| TGGCTATGAACAGCAACAGT+TGG | 0.497068 | 4.1:+68000280 | None:intergenic |
| GGCTATGAACAGCAACAGTT+GGG | 0.498761 | 4.1:+68000281 | None:intergenic |
| TGATATTTAGGTGGGATCAC+TGG | 0.502295 | 4.1:-67991807 | MS.gene83324:intron |
| TTGAATACGATAAATTCATC+AGG | 0.504742 | 4.1:-68002300 | MS.gene83324:CDS |
| TGTGGATATCACGGGGATGA+TGG | 0.505619 | 4.1:-68000950 | MS.gene83324:CDS |
| CACTATATTTATAGGAGGAA+TGG | 0.511735 | 4.1:+67995118 | None:intergenic |
| CTAATAGCTGTGGATATCAC+GGG | 0.512447 | 4.1:-68000958 | MS.gene83324:CDS |
| TTAAAGGGCAGGTTTCGATT+GGG | 0.512635 | 4.1:-68002125 | MS.gene83324:CDS |
| ATTCATTTGACGAGGCTAGC+AGG | 0.517525 | 4.1:-67995145 | MS.gene83324:CDS |
| GGATGCTGGTGTTAAAGGGC+AGG | 0.521810 | 4.1:-68002136 | MS.gene83324:CDS |
| CAATGAAGCTAATCAGGGAT+GGG | 0.526869 | 4.1:-67994286 | MS.gene83324:intron |
| AGTTCACACCTTTAGGTCAA+AGG | 0.532522 | 4.1:+67999019 | None:intergenic |
| ATAAATATAGTGCAAGAAAA+CGG | 0.535203 | 4.1:-67995107 | MS.gene83324:CDS |
| GCTAATAGCTGTGGATATCA+CGG | 0.539147 | 4.1:-68000959 | MS.gene83324:CDS |
| GATGATGGTTTCCTTTATCG+TGG | 0.540400 | 4.1:-68000935 | MS.gene83324:CDS |
| TATACCACTGGTGATATAGT+TGG | 0.543774 | 4.1:-68000881 | MS.gene83324:CDS |
| AATCTTCATGGTCATGATGT+TGG | 0.548801 | 4.1:-68002225 | MS.gene83324:CDS |
| TAATAAACAATCCTCTTCAT+CGG | 0.552789 | 4.1:+68002177 | None:intergenic |
| TGTTTCAATGAAGCTAATCA+GGG | 0.556130 | 4.1:-67994291 | MS.gene83324:intron |
| TTTGGTCCAACCTATACCAC+TGG | 0.557020 | 4.1:-68000893 | MS.gene83324:CDS |
| ATAAAGTTGCACCGATGAAG+AGG | 0.558081 | 4.1:-68002188 | MS.gene83324:CDS |
| TCTTGCACTATATTTATAGG+AGG | 0.562030 | 4.1:+67995113 | None:intergenic |
| TCAATGAAGCTAATCAGGGA+TGG | 0.567235 | 4.1:-67994287 | MS.gene83324:intron |
| ACTGGTGATATAGTTGGTGC+TGG | 0.567921 | 4.1:-68000875 | MS.gene83324:CDS |
| AATACGATAAATTCATCAGG+TGG | 0.579812 | 4.1:-68002297 | MS.gene83324:CDS |
| TTTAAGATGAGGAGACAACC+TGG | 0.587971 | 4.1:-68002087 | MS.gene83324:CDS |
| AGAAAACGGTATTGATGACC+AGG | 0.594424 | 4.1:-67995093 | MS.gene83324:CDS |
| GGTTTCCTTTATCGTGGACA+GGG | 0.594845 | 4.1:-68000929 | MS.gene83324:CDS |
| ATATCACCAGTGGTATAGGT+TGG | 0.595825 | 4.1:+68000887 | None:intergenic |
| TTAGGTGGGATCACTGGAGG+AGG | 0.600622 | 4.1:-67991801 | MS.gene83324:intron |
| TCACAGAAAGACTCTACGGC+AGG | 0.601062 | 4.1:-67995051 | MS.gene83324:intron |
| AATGAAGCTAATCAGGGATG+GGG | 0.603128 | 4.1:-67994285 | MS.gene83324:intron |
| ACAGCAACAGTTGGGAAAAG+GGG | 0.605059 | 4.1:+68000289 | None:intergenic |
| TGAATCACAGAAAGACTCTA+CGG | 0.618580 | 4.1:-67995055 | MS.gene83324:CDS |
| TACATTGAATTCATTTGACG+AGG | 0.619444 | 4.1:-67995153 | MS.gene83324:CDS |
| AGGTGGGAGGCTAATAGCTG+TGG | 0.620718 | 4.1:-68000968 | MS.gene83324:CDS |
| CATAGTGTAGCAGGTAGGAA+CGG | 0.636160 | 4.1:+67995186 | None:intergenic |
| TAACTGGCATTTGGTGACAC+AGG | 0.640519 | 4.1:+67998018 | None:intergenic |
| GTTATTTGATGATAGGTGGG+AGG | 0.644271 | 4.1:-68000981 | MS.gene83324:intron |
| ACAGTTTGTAAAGAAATGAA+GGG | 0.648698 | 4.1:-68000313 | MS.gene83324:CDS |
| TTTATCGTATTCAACTCACT+CGG | 0.656213 | 4.1:+68002309 | None:intergenic |
| CAATCAATGAAAGAAACCAA+CGG | 0.657872 | 4.1:-68002477 | None:intergenic |
| TGAACTAAATCCTCAAAGAG+AGG | 0.658307 | 4.1:+67991734 | None:intergenic |
| GAAGCAACAAATCAAAACAG+AGG | 0.661214 | 4.1:-67998046 | MS.gene83324:CDS |
| TTATCGTGGACAGGGTAAAG+GGG | 0.668081 | 4.1:-68000921 | MS.gene83324:CDS |
| TATTTAGGTGGGATCACTGG+AGG | 0.670003 | 4.1:-67991804 | MS.gene83324:intron |
| TTAAGATGAGGAGACAACCT+GGG | 0.670911 | 4.1:-68002086 | MS.gene83324:intron |
| TAGCACACCTCAGCAATCTG+GGG | 0.678597 | 4.1:+67994224 | None:intergenic |
| AGCACCAACTATATCACCAG+TGG | 0.702372 | 4.1:+68000877 | None:intergenic |
| GGTTTCGATTGGGTTTACGA+CGG | 0.708370 | 4.1:-68002115 | MS.gene83324:CDS |
| ATAGCCATAGTGTAGCAGGT+AGG | 0.718923 | 4.1:+67995181 | None:intergenic |
| ATGGTATCCCCAGATTGCTG+AGG | 0.724330 | 4.1:-67994231 | MS.gene83324:intron |
| TAATAGCTGTGGATATCACG+GGG | 0.725260 | 4.1:-68000957 | MS.gene83324:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 67991733 | 68002493 | 67991733 | ID=MS.gene83324 |
| chr4.1 | mRNA | 67991733 | 68002493 | 67991733 | ID=MS.gene83324.t1;Parent=MS.gene83324 |
| chr4.1 | exon | 68002087 | 68002493 | 68002087 | ID=MS.gene83324.t1.exon1;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 68002087 | 68002493 | 68002087 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| chr4.1 | exon | 68000842 | 68000988 | 68000842 | ID=MS.gene83324.t1.exon2;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 68000842 | 68000988 | 68000842 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| chr4.1 | exon | 68000271 | 68000357 | 68000271 | ID=MS.gene83324.t1.exon3;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 68000271 | 68000357 | 68000271 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| chr4.1 | exon | 67999028 | 67999073 | 67999028 | ID=MS.gene83324.t1.exon4;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 67999028 | 67999073 | 67999028 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| chr4.1 | exon | 67998015 | 67998091 | 67998015 | ID=MS.gene83324.t1.exon5;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 67998015 | 67998091 | 67998015 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| chr4.1 | exon | 67995052 | 67995214 | 67995052 | ID=MS.gene83324.t1.exon6;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 67995052 | 67995214 | 67995052 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| chr4.1 | exon | 67994232 | 67994300 | 67994232 | ID=MS.gene83324.t1.exon7;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 67994232 | 67994300 | 67994232 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| chr4.1 | exon | 67993058 | 67993117 | 67993058 | ID=MS.gene83324.t1.exon8;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 67993058 | 67993117 | 67993058 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| chr4.1 | exon | 67991733 | 67991819 | 67991733 | ID=MS.gene83324.t1.exon9;Parent=MS.gene83324.t1 |
| chr4.1 | CDS | 67991733 | 67991819 | 67991733 | ID=cds.MS.gene83324.t1;Parent=MS.gene83324.t1 |
| Gene Sequence |
| Protein sequence |
