Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84554.t1 | XP_003623208.1 | 96.9 | 225 | 7 | 0 | 1 | 225 | 1 | 225 | 2.50E-120 | 441.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84554.t1 | P32110 | 77.9 | 226 | 48 | 2 | 1 | 225 | 1 | 225 | 6.6e-98 | 358.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84554.t1 | G7L6I2 | 96.9 | 225 | 7 | 0 | 1 | 225 | 1 | 225 | 1.8e-120 | 441.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050381 | MS.gene84554 | 0.851556 | 8.22E-61 | -1.69E-46 |
| MS.gene050504 | MS.gene84554 | -0.805043 | 1.62E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene84554 | -0.803312 | 3.70E-49 | -1.69E-46 |
| MS.gene050871 | MS.gene84554 | -0.806431 | 8.25E-50 | -1.69E-46 |
| MS.gene050883 | MS.gene84554 | 0.821143 | 4.72E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene84554 | 0.825924 | 3.59E-54 | -1.69E-46 |
| MS.gene051138 | MS.gene84554 | 0.800108 | 1.68E-48 | -1.69E-46 |
| MS.gene051946 | MS.gene84554 | 0.821462 | 3.99E-53 | -1.69E-46 |
| MS.gene053549 | MS.gene84554 | 0.816952 | 4.24E-52 | -1.69E-46 |
| MS.gene054595 | MS.gene84554 | -0.80899 | 2.36E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene84554 | 0.81191 | 5.53E-51 | -1.69E-46 |
| MS.gene055124 | MS.gene84554 | 0.820964 | 5.19E-53 | -1.69E-46 |
| MS.gene055139 | MS.gene84554 | 0.867819 | 1.03E-65 | -1.69E-46 |
| MS.gene055154 | MS.gene84554 | 0.814032 | 1.89E-51 | -1.69E-46 |
| MS.gene055202 | MS.gene84554 | 0.814912 | 1.21E-51 | -1.69E-46 |
| MS.gene056310 | MS.gene84554 | 0.826072 | 3.32E-54 | -1.69E-46 |
| MS.gene056311 | MS.gene84554 | 0.803042 | 4.21E-49 | -1.69E-46 |
| MS.gene056313 | MS.gene84554 | 0.82455 | 7.60E-54 | -1.69E-46 |
| MS.gene056647 | MS.gene84554 | 0.822482 | 2.31E-53 | -1.69E-46 |
| MS.gene057014 | MS.gene84554 | 0.814467 | 1.52E-51 | -1.69E-46 |
| MS.gene057291 | MS.gene84554 | 0.807586 | 4.70E-50 | -1.69E-46 |
| MS.gene057293 | MS.gene84554 | 0.812753 | 3.62E-51 | -1.69E-46 |
| MS.gene058099 | MS.gene84554 | 0.816274 | 6.02E-52 | -1.69E-46 |
| MS.gene058171 | MS.gene84554 | 0.838472 | 2.82E-57 | -1.69E-46 |
| MS.gene058173 | MS.gene84554 | 0.827843 | 1.25E-54 | -1.69E-46 |
| MS.gene058175 | MS.gene84554 | 0.832694 | 8.17E-56 | -1.69E-46 |
| MS.gene059045 | MS.gene84554 | 0.82645 | 2.70E-54 | -1.69E-46 |
| MS.gene059223 | MS.gene84554 | 0.808781 | 2.62E-50 | -1.69E-46 |
| MS.gene059916 | MS.gene84554 | 0.819089 | 1.39E-52 | -1.69E-46 |
| MS.gene06077 | MS.gene84554 | -0.803969 | 2.70E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84554.t1 | MTR_7g065720 | 96.889 | 225 | 7 | 0 | 1 | 225 | 1 | 225 | 4.77e-161 | 444 |
| MS.gene84554.t1 | MTR_7g065660 | 76.339 | 224 | 52 | 1 | 1 | 224 | 1 | 223 | 3.79e-127 | 358 |
| MS.gene84554.t1 | MTR_7g065740 | 74.554 | 224 | 57 | 0 | 1 | 224 | 1 | 224 | 6.71e-126 | 355 |
| MS.gene84554.t1 | MTR_7g065750 | 74.779 | 226 | 56 | 1 | 1 | 225 | 1 | 226 | 4.47e-124 | 350 |
| MS.gene84554.t1 | MTR_7g065600 | 69.333 | 225 | 68 | 1 | 1 | 225 | 1 | 224 | 4.46e-114 | 325 |
| MS.gene84554.t1 | MTR_7g065640 | 71.875 | 224 | 44 | 2 | 1 | 224 | 1 | 205 | 5.20e-114 | 324 |
| MS.gene84554.t1 | MTR_7g065590 | 68.000 | 225 | 71 | 1 | 1 | 225 | 1 | 224 | 5.42e-113 | 322 |
| MS.gene84554.t1 | MTR_7g065290 | 69.778 | 225 | 67 | 1 | 1 | 225 | 1 | 224 | 5.53e-112 | 320 |
| MS.gene84554.t1 | MTR_7g065265 | 67.111 | 225 | 73 | 1 | 1 | 225 | 1 | 224 | 5.86e-106 | 305 |
| MS.gene84554.t1 | MTR_4g019790 | 64.732 | 224 | 78 | 1 | 1 | 224 | 1 | 223 | 1.03e-104 | 301 |
| MS.gene84554.t1 | MTR_7g065270 | 66.222 | 225 | 75 | 1 | 1 | 225 | 1 | 224 | 1.63e-104 | 301 |
| MS.gene84554.t1 | MTR_7g065630 | 68.304 | 224 | 66 | 2 | 1 | 224 | 19 | 237 | 2.45e-104 | 301 |
| MS.gene84554.t1 | MTR_7g065230 | 65.333 | 225 | 72 | 2 | 1 | 225 | 1 | 219 | 4.52e-100 | 290 |
| MS.gene84554.t1 | MTR_7g065260 | 63.274 | 226 | 81 | 2 | 1 | 225 | 8 | 232 | 1.43e-98 | 286 |
| MS.gene84554.t1 | MTR_4g019780 | 62.385 | 218 | 78 | 3 | 1 | 216 | 1 | 216 | 3.13e-96 | 280 |
| MS.gene84554.t1 | MTR_7g065710 | 65.385 | 208 | 67 | 2 | 8 | 215 | 5 | 207 | 3.39e-96 | 280 |
| MS.gene84554.t1 | MTR_7g065680 | 65.471 | 223 | 72 | 2 | 1 | 223 | 1 | 218 | 2.82e-91 | 267 |
| MS.gene84554.t1 | MTR_7g065700 | 65.581 | 215 | 69 | 2 | 1 | 215 | 1 | 210 | 1.32e-88 | 261 |
| MS.gene84554.t1 | MTR_8g087425 | 43.836 | 219 | 120 | 2 | 4 | 221 | 2 | 218 | 2.82e-66 | 204 |
| MS.gene84554.t1 | MTR_6g080440 | 46.890 | 209 | 108 | 2 | 7 | 214 | 3 | 209 | 2.92e-66 | 204 |
| MS.gene84554.t1 | MTR_3g099757 | 45.249 | 221 | 118 | 2 | 5 | 224 | 3 | 221 | 4.65e-65 | 201 |
| MS.gene84554.t1 | MTR_8g087410 | 47.196 | 214 | 109 | 3 | 1 | 213 | 1 | 211 | 3.05e-64 | 199 |
| MS.gene84554.t1 | MTR_1g090150 | 43.111 | 225 | 114 | 4 | 6 | 223 | 2 | 219 | 2.18e-61 | 192 |
| MS.gene84554.t1 | MTR_1g115195 | 45.498 | 211 | 113 | 1 | 7 | 215 | 3 | 213 | 7.76e-61 | 190 |
| MS.gene84554.t1 | MTR_1g090070 | 44.595 | 222 | 117 | 3 | 7 | 224 | 9 | 228 | 6.24e-59 | 186 |
| MS.gene84554.t1 | MTR_4g059730 | 44.393 | 214 | 114 | 2 | 6 | 216 | 3 | 214 | 5.88e-56 | 177 |
| MS.gene84554.t1 | MTR_1g090060 | 43.694 | 222 | 120 | 3 | 7 | 225 | 3 | 222 | 3.11e-55 | 176 |
| MS.gene84554.t1 | MTR_5g040430 | 40.807 | 223 | 123 | 3 | 1 | 216 | 1 | 221 | 2.17e-54 | 174 |
| MS.gene84554.t1 | MTR_1g090100 | 43.182 | 220 | 120 | 3 | 8 | 224 | 3 | 220 | 2.69e-53 | 171 |
| MS.gene84554.t1 | MTR_1g090090 | 44.286 | 210 | 112 | 2 | 7 | 213 | 3 | 210 | 2.94e-53 | 171 |
| MS.gene84554.t1 | MTR_5g037380 | 43.891 | 221 | 113 | 4 | 1 | 213 | 1 | 218 | 5.01e-53 | 171 |
| MS.gene84554.t1 | MTR_8g061950 | 43.182 | 220 | 116 | 4 | 5 | 216 | 3 | 221 | 1.05e-50 | 165 |
| MS.gene84554.t1 | MTR_2g070200 | 42.439 | 205 | 113 | 4 | 6 | 208 | 3 | 204 | 4.67e-48 | 157 |
| MS.gene84554.t1 | MTR_2g070150 | 42.718 | 206 | 110 | 5 | 6 | 208 | 3 | 203 | 5.86e-48 | 157 |
| MS.gene84554.t1 | MTR_2g070130 | 41.627 | 209 | 108 | 5 | 6 | 208 | 3 | 203 | 1.95e-46 | 153 |
| MS.gene84554.t1 | MTR_2g070120 | 42.029 | 207 | 114 | 5 | 6 | 210 | 3 | 205 | 5.38e-46 | 152 |
| MS.gene84554.t1 | MTR_3g467420 | 41.827 | 208 | 115 | 5 | 8 | 213 | 10 | 213 | 7.29e-46 | 152 |
| MS.gene84554.t1 | MTR_2g070140 | 41.837 | 196 | 106 | 6 | 21 | 213 | 2 | 192 | 8.77e-46 | 151 |
| MS.gene84554.t1 | MTR_2g070180 | 40.000 | 205 | 117 | 4 | 6 | 208 | 3 | 203 | 1.00e-44 | 149 |
| MS.gene84554.t1 | MTR_2g070210 | 40.566 | 212 | 118 | 6 | 6 | 213 | 3 | 210 | 3.01e-44 | 148 |
| MS.gene84554.t1 | MTR_8g056940 | 44.681 | 188 | 98 | 4 | 6 | 191 | 3 | 186 | 8.36e-44 | 146 |
| MS.gene84554.t1 | MTR_4g124130 | 39.640 | 222 | 123 | 6 | 5 | 221 | 2 | 217 | 8.51e-44 | 147 |
| MS.gene84554.t1 | MTR_3g467430 | 39.623 | 212 | 122 | 5 | 4 | 213 | 6 | 213 | 1.30e-43 | 146 |
| MS.gene84554.t1 | MTR_5g076900 | 37.559 | 213 | 127 | 5 | 3 | 213 | 2 | 210 | 2.82e-40 | 137 |
| MS.gene84554.t1 | MTR_2g070070 | 36.866 | 217 | 131 | 4 | 7 | 221 | 4 | 216 | 5.21e-40 | 137 |
| MS.gene84554.t1 | MTR_2g070060 | 35.849 | 212 | 124 | 5 | 7 | 213 | 4 | 208 | 6.54e-39 | 134 |
| MS.gene84554.t1 | MTR_0186s0030 | 49.645 | 141 | 68 | 2 | 6 | 145 | 3 | 141 | 4.13e-37 | 127 |
| MS.gene84554.t1 | MTR_2g070110 | 37.799 | 209 | 123 | 4 | 7 | 213 | 4 | 207 | 1.60e-36 | 128 |
| MS.gene84554.t1 | MTR_2g072120 | 49.153 | 59 | 29 | 1 | 51 | 108 | 2 | 60 | 1.49e-14 | 66.6 |
| MS.gene84554.t1 | MTR_5g037390 | 45.205 | 73 | 37 | 1 | 31 | 100 | 11 | 83 | 4.06e-13 | 63.5 |
| MS.gene84554.t1 | MTR_4g134370 | 27.184 | 206 | 121 | 6 | 21 | 209 | 29 | 222 | 1.76e-12 | 64.7 |
| MS.gene84554.t1 | MTR_4g134380 | 26.699 | 206 | 122 | 7 | 21 | 209 | 29 | 222 | 3.30e-12 | 63.9 |
| MS.gene84554.t1 | MTR_4g134380 | 32.847 | 137 | 81 | 5 | 21 | 153 | 29 | 158 | 3.22e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84554.t1 | AT3G09270 | 50.222 | 225 | 106 | 5 | 4 | 224 | 2 | 224 | 7.88e-75 | 226 |
| MS.gene84554.t1 | AT2G29420 | 49.091 | 220 | 109 | 2 | 3 | 221 | 5 | 222 | 1.12e-74 | 226 |
| MS.gene84554.t1 | AT2G29490 | 47.619 | 210 | 106 | 3 | 1 | 208 | 1 | 208 | 5.54e-60 | 188 |
| MS.gene84554.t1 | AT2G29480 | 45.238 | 210 | 111 | 3 | 1 | 208 | 1 | 208 | 5.26e-55 | 176 |
| MS.gene84554.t1 | AT2G29450 | 46.635 | 208 | 108 | 3 | 3 | 208 | 2 | 208 | 8.97e-55 | 175 |
| MS.gene84554.t1 | AT2G29460 | 43.049 | 223 | 123 | 3 | 1 | 221 | 1 | 221 | 1.22e-54 | 174 |
| MS.gene84554.t1 | AT1G10370 | 45.192 | 208 | 106 | 3 | 5 | 205 | 3 | 209 | 2.01e-54 | 174 |
| MS.gene84554.t1 | AT2G29440 | 45.714 | 210 | 109 | 4 | 1 | 208 | 1 | 207 | 3.09e-53 | 171 |
| MS.gene84554.t1 | AT2G29470 | 43.128 | 211 | 115 | 4 | 1 | 208 | 1 | 209 | 5.96e-53 | 170 |
| MS.gene84554.t1 | AT1G27130 | 41.593 | 226 | 122 | 5 | 1 | 218 | 1 | 224 | 3.63e-52 | 168 |
| MS.gene84554.t1 | AT1G10360 | 42.453 | 212 | 112 | 4 | 1 | 205 | 1 | 209 | 5.33e-51 | 165 |
| MS.gene84554.t1 | AT1G59700 | 45.933 | 209 | 104 | 5 | 5 | 205 | 4 | 211 | 1.03e-50 | 165 |
| MS.gene84554.t1 | AT1G59670 | 43.541 | 209 | 109 | 4 | 5 | 205 | 4 | 211 | 2.54e-50 | 164 |
| MS.gene84554.t1 | AT1G78340 | 43.333 | 210 | 113 | 4 | 6 | 213 | 3 | 208 | 2.79e-50 | 163 |
| MS.gene84554.t1 | AT1G69930 | 39.556 | 225 | 129 | 3 | 2 | 220 | 7 | 230 | 1.59e-49 | 162 |
| MS.gene84554.t1 | AT1G78380 | 44.279 | 201 | 106 | 4 | 7 | 205 | 4 | 200 | 3.01e-49 | 160 |
| MS.gene84554.t1 | AT1G78370 | 40.777 | 206 | 116 | 4 | 10 | 213 | 7 | 208 | 1.43e-48 | 159 |
| MS.gene84554.t1 | AT1G78320 | 40.930 | 215 | 121 | 5 | 5 | 217 | 2 | 212 | 1.78e-47 | 156 |
| MS.gene84554.t1 | AT1G17170 | 40.826 | 218 | 122 | 4 | 6 | 221 | 3 | 215 | 7.24e-47 | 154 |
| MS.gene84554.t1 | AT1G53680 | 38.498 | 213 | 127 | 3 | 1 | 211 | 1 | 211 | 1.38e-46 | 154 |
| MS.gene84554.t1 | AT5G62480 | 39.189 | 222 | 123 | 4 | 5 | 216 | 6 | 225 | 3.18e-46 | 153 |
| MS.gene84554.t1 | AT1G27140 | 38.961 | 231 | 130 | 5 | 1 | 222 | 1 | 229 | 1.01e-45 | 152 |
| MS.gene84554.t1 | AT1G69920 | 39.535 | 215 | 119 | 5 | 1 | 205 | 26 | 239 | 1.42e-45 | 152 |
| MS.gene84554.t1 | AT1G17180 | 40.952 | 210 | 118 | 5 | 6 | 213 | 3 | 208 | 1.36e-44 | 149 |
| MS.gene84554.t1 | AT1G78360 | 40.476 | 210 | 118 | 5 | 7 | 213 | 4 | 209 | 1.53e-41 | 141 |
| MS.gene84554.t1 | AT1G74590 | 33.778 | 225 | 141 | 4 | 1 | 219 | 1 | 223 | 2.02e-39 | 135 |
| MS.gene84554.t1 | AT3G43800 | 37.981 | 208 | 119 | 5 | 4 | 205 | 2 | 205 | 4.18e-39 | 135 |
| MS.gene84554.t1 | AT1G17190 | 37.674 | 215 | 126 | 5 | 1 | 213 | 1 | 209 | 2.64e-37 | 130 |
| MS.gene84554.t1 | AT1G78320 | 33.645 | 214 | 111 | 5 | 5 | 217 | 2 | 185 | 9.70e-31 | 112 |
| MS.gene84554.t1 | AT5G62480 | 30.769 | 221 | 117 | 4 | 5 | 216 | 6 | 199 | 9.10e-29 | 107 |
| MS.gene84554.t1 | AT5G62480 | 30.317 | 221 | 117 | 4 | 5 | 216 | 6 | 198 | 5.41e-28 | 105 |
| MS.gene84554.t1 | AT2G02390 | 26.906 | 223 | 135 | 7 | 3 | 209 | 4 | 214 | 5.62e-12 | 63.2 |
| MS.gene84554.t1 | AT2G02390 | 26.087 | 230 | 135 | 7 | 3 | 209 | 4 | 221 | 4.37e-11 | 60.8 |
Find 44 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCAATTCCCTTCAACTTT+AGG | 0.195855 | 7.3:-40486588 | None:intergenic |
| TTCAATTCCCTTCAACTTTA+GGG | 0.233116 | 7.3:-40486587 | None:intergenic |
| ATGGCTCCACTTGGAAAGTT+TGG | 0.257380 | 7.3:-40487257 | None:intergenic |
| AAAGAGCCTTGGCTCGATTT+TGG | 0.290434 | 7.3:+40486791 | MS.gene84554:CDS |
| CTGAAGGACAAGTTCTTTGC+TGG | 0.350924 | 7.3:+40487130 | MS.gene84554:CDS |
| TGGCTCCACTTGGAAAGTTT+GGG | 0.363283 | 7.3:-40487256 | None:intergenic |
| AAAATCGAGCCAAGGCTCTT+TGG | 0.372026 | 7.3:-40486789 | None:intergenic |
| CCTTTGATTCAAGACATTAC+AGG | 0.375169 | 7.3:+40487205 | MS.gene84554:CDS |
| TCTCAACCATCCAATTGTTA+AGG | 0.377126 | 7.3:+40487285 | MS.gene84554:CDS |
| TTCTTTGCTGGAGAGGAGAT+TGG | 0.380698 | 7.3:+40487142 | MS.gene84554:CDS |
| GTGAAGCTATTGGGAGCTAT+AGG | 0.394371 | 7.3:+40486535 | MS.gene84554:CDS |
| CAAGAAGAAGTGAAGCTATT+GGG | 0.403464 | 7.3:+40486526 | MS.gene84554:CDS |
| TCAAGAAGAAGTGAAGCTAT+TGG | 0.415262 | 7.3:+40486525 | MS.gene84554:CDS |
| ATCTGTACTCTACACACAAA+TGG | 0.417907 | 7.3:-40486562 | None:intergenic |
| TTGGATGGTTGAGAAATTCA+TGG | 0.441012 | 7.3:-40487276 | None:intergenic |
| ATCACAAGGGACTCTGATAT+AGG | 0.465216 | 7.3:-40486709 | None:intergenic |
| CGAGCCAAGGCTCTTTGGTA+TGG | 0.479917 | 7.3:-40486784 | None:intergenic |
| ACAGATTGCCCTAAAGTTGA+AGG | 0.480800 | 7.3:+40486579 | MS.gene84554:CDS |
| CCTGTAATGTCTTGAATCAA+AGG | 0.490769 | 7.3:-40487205 | None:intergenic |
| TTGAGTATATTGATGAGACT+TGG | 0.497242 | 7.3:+40486734 | MS.gene84554:CDS |
| CTTTGATTCAAGACATTACA+GGG | 0.498166 | 7.3:+40487206 | MS.gene84554:CDS |
| TCTGTACTCTACACACAAAT+GGG | 0.504085 | 7.3:-40486561 | None:intergenic |
| GAGCGCGAGAAGAATATTGA+AGG | 0.504203 | 7.3:+40487073 | MS.gene84554:CDS |
| GAGAAATTCATGGCTCCACT+TGG | 0.507464 | 7.3:-40487266 | None:intergenic |
| TATGGATCAGAAGGCAAGAT+GGG | 0.518997 | 7.3:-40486766 | None:intergenic |
| CTGTAATGTCTTGAATCAAA+GGG | 0.522980 | 7.3:-40487204 | None:intergenic |
| CAAATTTCTTGAAGAAAACA+TGG | 0.527059 | 7.3:+40486612 | MS.gene84554:CDS |
| TATAAACTGGGTTATACTTG+AGG | 0.528468 | 7.3:-40486654 | None:intergenic |
| TGGAGAGGAGATTGGAATTG+TGG | 0.538084 | 7.3:+40487150 | MS.gene84554:CDS |
| GGACAAGTTCTTTGCTGGAG+AGG | 0.567572 | 7.3:+40487135 | MS.gene84554:CDS |
| CAATATACTCAATAATCACA+AGG | 0.568482 | 7.3:-40486723 | None:intergenic |
| CTCATAGTGAGCTTTGAAGT+AGG | 0.574735 | 7.3:-40487338 | None:intergenic |
| GCTCTTTGGTATGGATCAGA+AGG | 0.575621 | 7.3:-40486775 | None:intergenic |
| GAATATTGAAGGAACAATTG+AGG | 0.577684 | 7.3:+40487084 | MS.gene84554:CDS |
| TGATCCATACCAAAGAGCCT+TGG | 0.580948 | 7.3:+40486780 | MS.gene84554:CDS |
| GTATGGATCAGAAGGCAAGA+TGG | 0.585713 | 7.3:-40486767 | None:intergenic |
| GATTCTTGAGAATGAGCTGA+AGG | 0.588727 | 7.3:+40487114 | MS.gene84554:CDS |
| GTCTAACTTCATCGACGACA+AGG | 0.594451 | 7.3:+40486813 | MS.gene84554:CDS |
| CAGATTGCCCTAAAGTTGAA+GGG | 0.596279 | 7.3:+40486580 | MS.gene84554:CDS |
| GTTAGACCAAAATCGAGCCA+AGG | 0.638172 | 7.3:-40486797 | None:intergenic |
| GCGCTCTTTCTCATCATCAA+CGG | 0.643070 | 7.3:-40487056 | None:intergenic |
| AGTGTCCCAAACTTTCCAAG+TGG | 0.647650 | 7.3:+40487251 | MS.gene84554:CDS |
| ATGGATCAGAAGGCAAGATG+GGG | 0.672832 | 7.3:-40486765 | None:intergenic |
| AATATACTCAATAATCACAA+GGG | 0.685010 | 7.3:-40486722 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTAAAAAAAAATCTAGCTT+CGG | + | chr7.3:40486956-40486975 | MS.gene84554:intron | 15.0% |
| !! | AATATACTCAATAATCACAA+GGG | - | chr7.3:40486725-40486744 | None:intergenic | 20.0% |
| ! | CAAATTTCTTGAAGAAAACA+TGG | + | chr7.3:40486612-40486631 | MS.gene84554:CDS | 25.0% |
| ! | CAATATACTCAATAATCACA+AGG | - | chr7.3:40486726-40486745 | None:intergenic | 25.0% |
| !! | ACTGGAATCTTTTTATAAAC+TGG | - | chr7.3:40486670-40486689 | None:intergenic | 25.0% |
| !! | CTGGAATCTTTTTATAAACT+GGG | - | chr7.3:40486669-40486688 | None:intergenic | 25.0% |
| !! | TATTTTCCTTAACAATTGGA+TGG | - | chr7.3:40487294-40487313 | None:intergenic | 25.0% |
| !! | TTTTCATTGTGAACAAAAAC+TGG | - | chr7.3:40486688-40486707 | None:intergenic | 25.0% |
| ATTCAATTCCCTTCAACTTT+AGG | - | chr7.3:40486591-40486610 | None:intergenic | 30.0% | |
| CTGTAATGTCTTGAATCAAA+GGG | - | chr7.3:40487207-40487226 | None:intergenic | 30.0% | |
| CTTTGATTCAAGACATTACA+GGG | + | chr7.3:40487206-40487225 | MS.gene84554:CDS | 30.0% | |
| GAATATTGAAGGAACAATTG+AGG | + | chr7.3:40487084-40487103 | MS.gene84554:CDS | 30.0% | |
| TATAAACTGGGTTATACTTG+AGG | - | chr7.3:40486657-40486676 | None:intergenic | 30.0% | |
| TATGCAAAGTTATTGTAACG+TGG | - | chr7.3:40486931-40486950 | None:intergenic | 30.0% | |
| TTCAATTCCCTTCAACTTTA+GGG | - | chr7.3:40486590-40486609 | None:intergenic | 30.0% | |
| TTGAGTATATTGATGAGACT+TGG | + | chr7.3:40486734-40486753 | MS.gene84554:CDS | 30.0% | |
| ! | GGCATATTTTCCTTAACAAT+TGG | - | chr7.3:40487298-40487317 | None:intergenic | 30.0% |
| ATCTGTACTCTACACACAAA+TGG | - | chr7.3:40486565-40486584 | None:intergenic | 35.0% | |
| CAAGAAGAAGTGAAGCTATT+GGG | + | chr7.3:40486526-40486545 | MS.gene84554:CDS | 35.0% | |
| CCTGTAATGTCTTGAATCAA+AGG | - | chr7.3:40487208-40487227 | None:intergenic | 35.0% | |
| CCTTTGATTCAAGACATTAC+AGG | + | chr7.3:40487205-40487224 | MS.gene84554:CDS | 35.0% | |
| TCAAGAAGAAGTGAAGCTAT+TGG | + | chr7.3:40486525-40486544 | MS.gene84554:CDS | 35.0% | |
| TCTCAACCATCCAATTGTTA+AGG | + | chr7.3:40487285-40487304 | MS.gene84554:CDS | 35.0% | |
| TCTGTACTCTACACACAAAT+GGG | - | chr7.3:40486564-40486583 | None:intergenic | 35.0% | |
| TTGGATGGTTGAGAAATTCA+TGG | - | chr7.3:40487279-40487298 | None:intergenic | 35.0% | |
| ! | CAGCTGTGTATATAGCTTTT+TGG | + | chr7.3:40487179-40487198 | MS.gene84554:CDS | 35.0% |
| ACAGATTGCCCTAAAGTTGA+AGG | + | chr7.3:40486579-40486598 | MS.gene84554:CDS | 40.0% | |
| ATCACAAGGGACTCTGATAT+AGG | - | chr7.3:40486712-40486731 | None:intergenic | 40.0% | |
| CAGATTGCCCTAAAGTTGAA+GGG | + | chr7.3:40486580-40486599 | MS.gene84554:CDS | 40.0% | |
| GATTCTTGAGAATGAGCTGA+AGG | + | chr7.3:40487114-40487133 | MS.gene84554:CDS | 40.0% | |
| TAGGCAAAAAGAGGTTCTCT+AGG | - | chr7.3:40487322-40487341 | None:intergenic | 40.0% | |
| TATGGATCAGAAGGCAAGAT+GGG | - | chr7.3:40486769-40486788 | None:intergenic | 40.0% | |
| !! | CTCATAGTGAGCTTTGAAGT+AGG | - | chr7.3:40487341-40487360 | None:intergenic | 40.0% |
| !! | GCTTTGAAGTAGGCAAAAAG+AGG | - | chr7.3:40487331-40487350 | None:intergenic | 40.0% |
| !!! | GACTGATGCAGTTTTAGTTG+CGG | + | chr7.3:40486905-40486924 | MS.gene84554:intron | 40.0% |
| AAAATCGAGCCAAGGCTCTT+TGG | - | chr7.3:40486792-40486811 | None:intergenic | 45.0% | |
| AGTGTCCCAAACTTTCCAAG+TGG | + | chr7.3:40487251-40487270 | MS.gene84554:CDS | 45.0% | |
| ATGGATCAGAAGGCAAGATG+GGG | - | chr7.3:40486768-40486787 | None:intergenic | 45.0% | |
| ATGGCTCCACTTGGAAAGTT+TGG | - | chr7.3:40487260-40487279 | None:intergenic | 45.0% | |
| CTGAAGGACAAGTTCTTTGC+TGG | + | chr7.3:40487130-40487149 | MS.gene84554:CDS | 45.0% | |
| GAGAAATTCATGGCTCCACT+TGG | - | chr7.3:40487269-40487288 | None:intergenic | 45.0% | |
| GAGCGCGAGAAGAATATTGA+AGG | + | chr7.3:40487073-40487092 | MS.gene84554:CDS | 45.0% | |
| GCAAAAAGAGGTTCTCTAGG+TGG | - | chr7.3:40487319-40487338 | None:intergenic | 45.0% | |
| GCGCTCTTTCTCATCATCAA+CGG | - | chr7.3:40487059-40487078 | None:intergenic | 45.0% | |
| GCTCTTTGGTATGGATCAGA+AGG | - | chr7.3:40486778-40486797 | None:intergenic | 45.0% | |
| GTATGGATCAGAAGGCAAGA+TGG | - | chr7.3:40486770-40486789 | None:intergenic | 45.0% | |
| GTCTAACTTCATCGACGACA+AGG | + | chr7.3:40486813-40486832 | MS.gene84554:CDS | 45.0% | |
| GTGAAGCTATTGGGAGCTAT+AGG | + | chr7.3:40486535-40486554 | MS.gene84554:CDS | 45.0% | |
| GTTAGACCAAAATCGAGCCA+AGG | - | chr7.3:40486800-40486819 | None:intergenic | 45.0% | |
| TGATCCATACCAAAGAGCCT+TGG | + | chr7.3:40486780-40486799 | MS.gene84554:CDS | 45.0% | |
| TGCAGTCGCGTTGAAATACT+TGG | + | chr7.3:40486857-40486876 | MS.gene84554:intron | 45.0% | |
| TGGAGAGGAGATTGGAATTG+TGG | + | chr7.3:40487150-40487169 | MS.gene84554:CDS | 45.0% | |
| TGGCTCCACTTGGAAAGTTT+GGG | - | chr7.3:40487259-40487278 | None:intergenic | 45.0% | |
| TTCTTTGCTGGAGAGGAGAT+TGG | + | chr7.3:40487142-40487161 | MS.gene84554:CDS | 45.0% | |
| !! | AAAGAGCCTTGGCTCGATTT+TGG | + | chr7.3:40486791-40486810 | MS.gene84554:CDS | 45.0% |
| GGACAAGTTCTTTGCTGGAG+AGG | + | chr7.3:40487135-40487154 | MS.gene84554:CDS | 50.0% | |
| CGAGCCAAGGCTCTTTGGTA+TGG | - | chr7.3:40486787-40486806 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 40486514 | 40487381 | 40486514 | ID=MS.gene84554 |
| chr7.3 | mRNA | 40486514 | 40487381 | 40486514 | ID=MS.gene84554.t1;Parent=MS.gene84554 |
| chr7.3 | exon | 40486514 | 40486834 | 40486514 | ID=MS.gene84554.t1.exon1;Parent=MS.gene84554.t1 |
| chr7.3 | CDS | 40486514 | 40486834 | 40486514 | ID=cds.MS.gene84554.t1;Parent=MS.gene84554.t1 |
| chr7.3 | exon | 40487025 | 40487381 | 40487025 | ID=MS.gene84554.t1.exon2;Parent=MS.gene84554.t1 |
| chr7.3 | CDS | 40487025 | 40487381 | 40487025 | ID=cds.MS.gene84554.t1;Parent=MS.gene84554.t1 |
| Gene Sequence |
| Protein sequence |
