AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85273

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85273.t1 MTR_2g099030 99.471 378 2 0 1 378 30 407 0.0 784
MS.gene85273.t1 MTR_2g087780 46.825 378 189 7 1 374 23 392 3.25e-110 328
MS.gene85273.t1 MTR_7g490350 49.408 338 160 5 37 372 36 364 2.83e-106 317
MS.gene85273.t1 MTR_7g090300 48.910 321 154 4 53 372 53 364 6.68e-104 311
MS.gene85273.t1 MTR_7g490330 48.338 331 123 4 43 372 37 320 1.37e-101 303
MS.gene85273.t1 MTR_4g050190 43.351 376 204 5 1 374 20 388 1.83e-101 305
MS.gene85273.t1 MTR_5g091980 45.483 321 124 5 53 372 48 318 8.45e-85 261
MS.gene85273.t1 MTR_7g090320 38.056 360 202 6 21 367 26 377 2.49e-83 259
MS.gene85273.t1 MTR_7g090310 39.222 334 181 7 47 367 52 376 2.62e-82 256
MS.gene85273.t1 MTR_4g112670 33.533 334 193 4 41 373 27 332 9.97e-62 202
MS.gene85273.t1 MTR_4g112650 33.136 338 193 4 41 375 26 333 2.95e-59 195
MS.gene85273.t1 MTR_4g112630 33.731 335 178 5 41 371 26 320 1.48e-58 193
MS.gene85273.t1 MTR_2g058450 33.531 337 192 6 41 375 28 334 4.27e-55 184
MS.gene85273.t1 MTR_4g112620 29.518 332 167 5 41 371 30 295 7.30e-43 151
MS.gene85273.t1 MTR_8g074270 32.911 316 165 9 33 347 60 329 4.46e-42 151
MS.gene85273.t1 MTR_8g074170 34.317 271 148 6 33 303 60 300 4.08e-41 149
MS.gene85273.t1 MTR_8g074210 33.721 258 143 6 48 305 73 302 3.87e-39 143
MS.gene85273.t1 MTR_2g042370 29.329 283 189 6 95 376 84 356 4.70e-36 135
MS.gene85273.t1 MTR_2g058410 32.031 256 147 4 122 375 1 231 6.07e-34 126
MS.gene85273.t1 MTR_2g042430 26.899 316 209 6 64 378 75 369 3.36e-33 127
MS.gene85273.t1 MTR_2g042420 27.445 317 202 7 64 376 56 348 1.53e-31 122
MS.gene85273.t1 MTR_0003s0530 29.268 205 121 3 165 368 1 182 4.26e-25 101
MS.gene85273.t1 MTR_1g075865 41.905 105 61 0 170 274 7 111 1.27e-24 98.2
MS.gene85273.t1 MTR_8g074320 32.544 169 104 6 78 242 58 220 9.57e-20 87.4
MS.gene85273.t1 MTR_4g112840 38.776 98 60 0 198 295 63 160 1.19e-18 82.8
MS.gene85273.t1 MTR_2g058430 35.789 95 58 1 115 206 54 148 1.23e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85273.t1 AT3G59530 77.778 378 73 1 1 378 32 398 0.0 634
MS.gene85273.t1 AT3G59530 77.778 378 73 1 1 378 32 398 0.0 634
MS.gene85273.t1 AT3G59530 77.778 378 73 1 1 378 32 398 0.0 634
MS.gene85273.t1 AT5G22020 45.067 375 198 6 1 374 25 392 1.47e-106 319
MS.gene85273.t1 AT5G22020 45.067 375 198 6 1 374 25 392 1.47e-106 319
MS.gene85273.t1 AT5G22020 45.067 375 198 6 1 374 25 392 1.47e-106 319
MS.gene85273.t1 AT3G57030 49.371 318 149 5 53 367 56 364 9.88e-105 313
MS.gene85273.t1 AT1G08470 41.995 381 209 7 1 378 19 390 1.55e-100 303
MS.gene85273.t1 AT3G57020 41.250 320 174 5 51 367 51 359 4.35e-81 253
MS.gene85273.t1 AT3G57010 42.105 323 167 7 51 367 52 360 3.07e-78 246
MS.gene85273.t1 AT2G41300 41.667 312 167 5 59 367 84 383 1.91e-76 241
MS.gene85273.t1 AT3G57020 39.375 320 166 5 51 367 51 345 2.30e-75 238
MS.gene85273.t1 AT2G41290 39.939 328 176 8 51 367 49 366 2.06e-71 228
MS.gene85273.t1 AT1G74020 32.915 319 171 9 53 367 38 317 8.34e-50 171
MS.gene85273.t1 AT1G74010 33.129 326 178 9 44 367 28 315 8.05e-47 162
MS.gene85273.t1 AT1G74000 33.438 320 168 11 53 367 40 319 5.39e-46 160
MS.gene85273.t1 AT3G51440 30.746 335 183 9 54 378 70 365 6.45e-33 127
MS.gene85273.t1 AT3G51430 28.657 335 190 12 54 378 70 365 2.60e-31 122
MS.gene85273.t1 AT3G51430 28.060 335 190 12 54 378 70 363 5.76e-31 121
MS.gene85273.t1 AT3G51450 29.080 337 186 12 54 378 70 365 3.63e-30 119
MS.gene85273.t1 AT3G51420 26.727 333 190 12 54 375 70 359 4.15e-30 119

Find 95 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATTCGAAAATGAAGTATT+TGG 0.199215 2.4:-5068155 MS.gene85273:CDS
TTCTTCATGACTTGTTTATA+TGG 0.232247 2.4:+5068224 None:intergenic
CATTCCACTGTTCCATCTTT+AGG 0.248214 2.4:+5066712 None:intergenic
GCCAACAAGCTCATTCTTAC+TGG 0.269587 2.4:+5066547 None:intergenic
TGCATATTATGGTCTTCTTA+TGG 0.279511 2.4:-5067565 MS.gene85273:CDS
TTCTCATGTTTCCATTGCTT+TGG 0.287013 2.4:+5067651 None:intergenic
CAAGACAACATGAGTTGTTT+TGG 0.309300 2.4:+5067104 None:intergenic
TACATAGCAGATGCATATTA+TGG 0.318723 2.4:-5067576 MS.gene85273:CDS
AATTGGTGGTTTCGGTAAAA+AGG 0.329225 2.4:+5067028 None:intergenic
TCATTCATTCTTACGTTGTC+TGG 0.331202 2.4:+5066667 None:intergenic
AAACCTGCAATTGGTGGTTT+CGG 0.351201 2.4:+5067020 None:intergenic
ATAAACAAGTCATGAAGAAT+TGG 0.371511 2.4:-5068220 MS.gene85273:CDS
AAGAGGAGAAAGGAAAGCTT+TGG 0.372961 2.4:-5066408 MS.gene85273:CDS
TTCGAGATTTGCACTCCATT+AGG 0.378181 2.4:+5067066 None:intergenic
AACGTAAGAATGAATGAAAA+AGG 0.383654 2.4:-5066661 MS.gene85273:CDS
CTTCTTATGGTTGGACCAAA+TGG 0.385238 2.4:-5067552 MS.gene85273:CDS
AACACGACCATCGGCTAAAC+CGG 0.389147 2.4:+5068088 None:intergenic
AAGATCATTTGCAAAGAGAA+TGG 0.401336 2.4:+5067488 None:intergenic
TGGTAGGAGGATCATATCTA+AGG 0.402934 2.4:+5067124 None:intergenic
CCTAGTGGACCTAAATGAAA+AGG 0.405868 2.4:+5068284 None:intergenic
ATGACAGGCTAATGAAACTT+TGG 0.408963 2.4:-5066744 MS.gene85273:intron
TATTATGGTCTTCTTATGGT+TGG 0.409388 2.4:-5067561 MS.gene85273:CDS
TTACCGAAACCACCAATTGC+AGG 0.427515 2.4:-5067023 MS.gene85273:intron
ATCTTGATATTCACAAGAAC+GGG 0.427957 2.4:-5067470 MS.gene85273:CDS
CTAATGAAACTTTGGATAGA+AGG 0.434236 2.4:-5066736 MS.gene85273:CDS
CCATCGGCTAAACCGGTGTA+AGG 0.444345 2.4:+5068095 None:intergenic
GCAATAGATTGTTGTCGAAC+TGG 0.446574 2.4:-5066625 MS.gene85273:CDS
CTTACGTTGTCTGGAAATCC+TGG 0.449914 2.4:+5066676 None:intergenic
TGGTCGTGTTGTCCGATGGA+TGG 0.450319 2.4:-5068075 MS.gene85273:CDS
GGTGTAGCCAGTCCTCCATT+TGG 0.451029 2.4:+5067537 None:intergenic
GCCAGTAAGAATGAGCTTGT+TGG 0.451834 2.4:-5066548 MS.gene85273:CDS
AGATCATTTGCAAAGAGAAT+GGG 0.452460 2.4:+5067489 None:intergenic
GGTCGTGTTGTCCGATGGAT+GGG 0.455520 2.4:-5068074 MS.gene85273:CDS
CAAAACAACTCATGTTGTCT+TGG 0.456064 2.4:-5067103 MS.gene85273:CDS
TTAAGATTTGACAAAGAGAG+TGG 0.459619 2.4:-5067606 MS.gene85273:CDS
CCTTCAACATGGGTTGCCAA+TGG 0.466610 2.4:+5067516 None:intergenic
GCTGGTTAGTGAAGTTAAAG+AGG 0.470251 2.4:-5066425 MS.gene85273:CDS
TTGCAGTTGTCACATCCAAT+TGG 0.472198 2.4:-5068028 MS.gene85273:intron
GAATGCGTAGCTGACCTTCC+AGG 0.474792 2.4:-5066694 MS.gene85273:CDS
GAGCTTGTTGGCTAAAGCTA+TGG 0.475927 2.4:-5066536 MS.gene85273:CDS
ATTGCACTTCTTGATGATAA+CGG 0.476011 2.4:-5066493 MS.gene85273:CDS
ATGAGCAGACTCGGACTTCA+CGG 0.478710 2.4:-5068185 MS.gene85273:CDS
GAGAAAGGAAAGCTTTGGAT+TGG 0.479363 2.4:-5066403 MS.gene85273:CDS
TTTATATTGTTGGAAGGAGA+AGG 0.479834 2.4:-5067159 MS.gene85273:CDS
CTTTGTCAAATCTTAAACCA+AGG 0.491441 2.4:+5067613 None:intergenic
GTCACTTGAGTTCGATAACA+TGG 0.494746 2.4:-5068126 MS.gene85273:CDS
AGCTTGTTGGCTAAAGCTAT+GGG 0.498617 2.4:-5066535 MS.gene85273:CDS
CGAAAATGAAGTATTTGGAC+CGG 0.503096 2.4:-5068150 MS.gene85273:CDS
AAAACATGTGGGCGTCCCCT+TGG 0.510216 2.4:-5067630 MS.gene85273:CDS
AGTCTGCTCATGTTGTCCTT+AGG 0.510827 2.4:+5068197 None:intergenic
TGGATAGAAGGTCCTAAAGA+TGG 0.515483 2.4:-5066724 MS.gene85273:CDS
GTCGTGTTGTCCGATGGATG+GGG 0.520866 2.4:-5068073 MS.gene85273:CDS
AGTGAAGTTAAAGAGGAGAA+AGG 0.523089 2.4:-5066418 MS.gene85273:CDS
CCATTGGCAACCCATGTTGA+AGG 0.526859 2.4:-5067516 MS.gene85273:CDS
TGGAGGACTGGCTACACCAT+TGG 0.527000 2.4:-5067532 MS.gene85273:CDS
ACAACTCATGTTGTCTTGGA+TGG 0.528253 2.4:-5067099 MS.gene85273:CDS
CTAGTGGACCTAAATGAAAA+GGG 0.529354 2.4:+5068285 None:intergenic
TTGTTACTCAAGACTTCTTG+AGG 0.540862 2.4:+5066601 None:intergenic
TTTGTCAAATCTTAAACCAA+GGG 0.542290 2.4:+5067614 None:intergenic
TTGTTGGAAGGAGAAGGAAC+AGG 0.544523 2.4:-5067153 MS.gene85273:CDS
CCTTACACCGGTTTAGCCGA+TGG 0.545860 2.4:-5068095 MS.gene85273:CDS
GGTGCAACGTTGTGTTTCAC+AGG 0.546534 2.4:+5068245 None:intergenic
CCGATGGTCGTGTTGTCCGA+TGG 0.555843 2.4:-5068079 MS.gene85273:CDS
AAGTCTTGAGTAACAATCCA+TGG 0.565030 2.4:-5066594 MS.gene85273:CDS
GGTTGGACCAAATGGAGGAC+TGG 0.566119 2.4:-5067544 MS.gene85273:CDS
GAGTTCGATAACATGGGCCG+TGG 0.566184 2.4:-5068119 MS.gene85273:CDS
AAGGACAACATGAGCAGACT+CGG 0.574652 2.4:-5068194 MS.gene85273:CDS
ATTCTACAACTCCAAAGCAA+TGG 0.574802 2.4:-5067662 MS.gene85273:CDS
GATCTTGATATTCACAAGAA+CGG 0.577423 2.4:-5067471 MS.gene85273:CDS
GACTGAGAAGATTTGCATGA+GGG 0.580450 2.4:-5067691 MS.gene85273:CDS
ATGGGCCGTGGCCCTTACAC+CGG 0.582691 2.4:-5068107 MS.gene85273:CDS
CCATCGGACAACACGACCAT+CGG 0.587160 2.4:+5068079 None:intergenic
CTAAATTCATGTTCTCCTAG+TGG 0.592914 2.4:+5068269 None:intergenic
CTTATGGTTGGACCAAATGG+AGG 0.594581 2.4:-5067549 MS.gene85273:CDS
AGAAGGCAAAGTGATGAAGC+TGG 0.599296 2.4:-5066443 MS.gene85273:CDS
AGTCATGAAGAATTGGCCTA+AGG 0.608763 2.4:-5068213 MS.gene85273:CDS
GGACTGAGAAGATTTGCATG+AGG 0.612890 2.4:-5067692 MS.gene85273:CDS
CTAAACCGGTGTAAGGGCCA+CGG 0.618440 2.4:+5068102 None:intergenic
TTATCGAACTCAAGTGACTC+CGG 0.620156 2.4:+5068131 None:intergenic
TGGAAACATGAGAAAACATG+TGG 0.620861 2.4:-5067642 MS.gene85273:CDS
TGAGTAACAATCCATGGTTG+AGG 0.621036 2.4:-5066588 MS.gene85273:CDS
ACTGAGAAGATTTGCATGAG+GGG 0.622020 2.4:-5067690 MS.gene85273:CDS
GCTTGTGTCAGTGAAGAAGA+TGG 0.622137 2.4:+5067446 None:intergenic
AGCTCATTCTTACTGGCAAG+CGG 0.627455 2.4:+5066554 None:intergenic
GAAGTTCTTGAAGATCGAGA+AGG 0.628335 2.4:-5066460 MS.gene85273:CDS
CATCGGCTAAACCGGTGTAA+GGG 0.633079 2.4:+5068096 None:intergenic
CGTTGTCTGGAAATCCTGGA+AGG 0.633711 2.4:+5066680 None:intergenic
TCGTGTTGTCCGATGGATGG+GGG 0.655214 2.4:-5068072 MS.gene85273:CDS
TTGTCAAATCTTAAACCAAG+GGG 0.656805 2.4:+5067615 None:intergenic
AAGTAAATACTCCTCAACCA+TGG 0.660467 2.4:+5066577 None:intergenic
TCACTTGAGTTCGATAACAT+GGG 0.668236 2.4:-5068125 MS.gene85273:CDS
AGGTCCTAAAGATGGAACAG+TGG 0.678364 2.4:-5066716 MS.gene85273:CDS
TGGAAGGAGAAGGAACAGGT+AGG 0.689818 2.4:-5067149 MS.gene85273:CDS
GGAAACATGAGAAAACATGT+GGG 0.709710 2.4:-5067641 MS.gene85273:CDS
GCTTGTTGGCTAAAGCTATG+GGG 0.726642 2.4:-5066534 MS.gene85273:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTTAATATAAAATA+CGG + chr2.4:5067332-5067351 None:intergenic 0.0%
!! AAAAAATCTACAATAAAATT+TGG - chr2.4:5067345-5067364 MS.gene85273:intron 10.0%
!!! ACATAATATTTTAGACTTAA+TGG + chr2.4:5066886-5066905 None:intergenic 15.0%
!!! TAATGAATTAGTTATTTTGT+TGG + chr2.4:5067877-5067896 None:intergenic 15.0%
!! AATCTACAATAAAATTTGGA+CGG - chr2.4:5067349-5067368 MS.gene85273:intron 20.0%
!! CTTAGCTGAAATAAATAAAT+AGG + chr2.4:5067433-5067452 None:intergenic 20.0%
!! TCATTAAATTCTTTAATGAC+AGG - chr2.4:5067891-5067910 MS.gene85273:intron 20.0%
!!! TGAAACTTGGTATTTTTAAA+AGG - chr2.4:5066937-5066956 MS.gene85273:intron 20.0%
! AAACAATAACACTCATCATA+AGG + chr2.4:5067457-5067476 None:intergenic 25.0%
! AAATAAATAAATAGGATCCC+TGG + chr2.4:5067425-5067444 None:intergenic 25.0%
! AACGTAAGAATGAATGAAAA+AGG - chr2.4:5067989-5068008 MS.gene85273:intron 25.0%
! ATAAACAAGTCATGAAGAAT+TGG - chr2.4:5066430-5066449 MS.gene85273:CDS 25.0%
! ATCATTTGATCTCAATTCAA+CGG + chr2.4:5067295-5067314 None:intergenic 25.0%
! GAGAAAATGCAAAATAAGTA+CGG + chr2.4:5067688-5067707 None:intergenic 25.0%
! TGTAATACTTGAGGTTAAAT+GGG - chr2.4:5067715-5067734 MS.gene85273:intron 25.0%
! TTGTAATACTTGAGGTTAAA+TGG - chr2.4:5067714-5067733 MS.gene85273:intron 25.0%
! TTTGTCAAATCTTAAACCAA+GGG + chr2.4:5067039-5067058 None:intergenic 25.0%
!! GAATTCGAAAATGAAGTATT+TGG - chr2.4:5066495-5066514 MS.gene85273:CDS 25.0%
!! TACATTGTGATCTTTTATCT+TGG - chr2.4:5067756-5067775 MS.gene85273:intron 25.0%
!!! CACTTTTTTATATTGTTGGA+AGG - chr2.4:5067485-5067504 MS.gene85273:CDS 25.0%
!!! CGCTAAGGTTTTTTAAAAAA+TGG + chr2.4:5067791-5067810 None:intergenic 25.0%
!!! TGCACACTTTTTTATATTGT+TGG - chr2.4:5067481-5067500 MS.gene85273:CDS 25.0%
!!! TTCTTCATGACTTGTTTATA+TGG + chr2.4:5066429-5066448 None:intergenic 25.0%
AAGATCATTTGCAAAGAGAA+TGG + chr2.4:5067165-5067184 None:intergenic 30.0%
AGATCATTTGCAAAGAGAAT+GGG + chr2.4:5067164-5067183 None:intergenic 30.0%
ATCTTGATATTCACAAGAAC+GGG - chr2.4:5067180-5067199 MS.gene85273:intron 30.0%
CTAATGAAACTTTGGATAGA+AGG - chr2.4:5067914-5067933 MS.gene85273:intron 30.0%
CTTTGTCAAATCTTAAACCA+AGG + chr2.4:5067040-5067059 None:intergenic 30.0%
GATCTTGATATTCACAAGAA+CGG - chr2.4:5067179-5067198 MS.gene85273:intron 30.0%
TACATAGCAGATGCATATTA+TGG - chr2.4:5067074-5067093 MS.gene85273:CDS 30.0%
TATTATGGTCTTCTTATGGT+TGG - chr2.4:5067089-5067108 MS.gene85273:CDS 30.0%
TGCATATTATGGTCTTCTTA+TGG - chr2.4:5067085-5067104 MS.gene85273:CDS 30.0%
TTAAGATTTGACAAAGAGAG+TGG - chr2.4:5067044-5067063 MS.gene85273:CDS 30.0%
TTGTCAAATCTTAAACCAAG+GGG + chr2.4:5067038-5067057 None:intergenic 30.0%
TTTATATTGTTGGAAGGAGA+AGG - chr2.4:5067491-5067510 MS.gene85273:CDS 30.0%
! ATTGCACTTCTTGATGATAA+CGG - chr2.4:5068157-5068176 MS.gene85273:CDS 30.0%
! GAATGAAAAAGGACAGTTTT+GGG - chr2.4:5068000-5068019 MS.gene85273:intron 30.0%
! TGAATGAAAAAGGACAGTTT+TGG - chr2.4:5067999-5068018 MS.gene85273:intron 30.0%
!! GTTATTTTGTTGGACGAATT+TGG + chr2.4:5067867-5067886 None:intergenic 30.0%
AAGTAAATACTCCTCAACCA+TGG + chr2.4:5068076-5068095 None:intergenic 35.0%
AAGTCTTGAGTAACAATCCA+TGG - chr2.4:5068056-5068075 MS.gene85273:CDS 35.0%
AGTGAAGTTAAAGAGGAGAA+AGG - chr2.4:5068232-5068251 MS.gene85273:CDS 35.0%
AGTTAAAAACCTGCAATTGG+TGG + chr2.4:5067639-5067658 None:intergenic 35.0%
ATAAATAGGATCCCTGGATT+CGG + chr2.4:5067419-5067438 None:intergenic 35.0%
ATGACAGGCTAATGAAACTT+TGG - chr2.4:5067906-5067925 MS.gene85273:intron 35.0%
ATTCTACAACTCCAAAGCAA+TGG - chr2.4:5066988-5067007 MS.gene85273:intron 35.0%
ATTGCAATGTGTCTCTCTAT+AGG + chr2.4:5066757-5066776 None:intergenic 35.0%
CAAAACAACTCATGTTGTCT+TGG - chr2.4:5067547-5067566 MS.gene85273:CDS 35.0%
CATTAGCATGAATGCACATA+TGG + chr2.4:5066919-5066938 None:intergenic 35.0%
CTAAATTCATGTTCTCCTAG+TGG + chr2.4:5066384-5066403 None:intergenic 35.0%
GCATTCATGCTAATGAAACT+TGG - chr2.4:5066924-5066943 MS.gene85273:intron 35.0%
GGAAACATGAGAAAACATGT+GGG - chr2.4:5067009-5067028 MS.gene85273:intron 35.0%
TCACTTGAGTTCGATAACAT+GGG - chr2.4:5066525-5066544 MS.gene85273:CDS 35.0%
TCATTCATTCTTACGTTGTC+TGG + chr2.4:5067986-5068005 None:intergenic 35.0%
TCTGCAACTTGTAATACTTG+AGG - chr2.4:5067706-5067725 MS.gene85273:intron 35.0%
TGCAGTTAAAAACCTGCAAT+TGG + chr2.4:5067642-5067661 None:intergenic 35.0%
TGGAAACATGAGAAAACATG+TGG - chr2.4:5067008-5067027 MS.gene85273:intron 35.0%
TTCTCATGTTTCCATTGCTT+TGG + chr2.4:5067002-5067021 None:intergenic 35.0%
TTGTTACTCAAGACTTCTTG+AGG + chr2.4:5068052-5068071 None:intergenic 35.0%
! AATTGGTGGTTTCGGTAAAA+AGG + chr2.4:5067625-5067644 None:intergenic 35.0%
! CAAGACAACATGAGTTGTTT+TGG + chr2.4:5067549-5067568 None:intergenic 35.0%
! CGAAAATGAAGTATTTGGAC+CGG - chr2.4:5066500-5066519 MS.gene85273:CDS 35.0%
! CTAGTGGACCTAAATGAAAA+GGG + chr2.4:5066368-5066387 None:intergenic 35.0%
! TCTTGATGATACTCACCAAT+TGG + chr2.4:5066640-5066659 None:intergenic 35.0%
!! AATGGGTTTTCCTTCAACAT+GGG + chr2.4:5067147-5067166 None:intergenic 35.0%
!!! ACAACATGAGTTGTTTTGGT+AGG + chr2.4:5067545-5067564 None:intergenic 35.0%
!!! GATGGTCTTGTTTTTCCTAA+TGG - chr2.4:5067569-5067588 MS.gene85273:CDS 35.0%
AAACCTTAGCGACACGAAAT+TGG - chr2.4:5067800-5067819 MS.gene85273:intron 40.0%
AAGAGGAGAAAGGAAAGCTT+TGG - chr2.4:5068242-5068261 MS.gene85273:CDS 40.0%
ACAACTCATGTTGTCTTGGA+TGG - chr2.4:5067551-5067570 MS.gene85273:CDS 40.0%
ACTGAGAAGATTTGCATGAG+GGG - chr2.4:5066960-5066979 MS.gene85273:intron 40.0%
CATTCCACTGTTCCATCTTT+AGG + chr2.4:5067941-5067960 None:intergenic 40.0%
CTTCTTATGGTTGGACCAAA+TGG - chr2.4:5067098-5067117 MS.gene85273:CDS 40.0%
GAAGTTCTTGAAGATCGAGA+AGG - chr2.4:5068190-5068209 MS.gene85273:CDS 40.0%
GACTGAGAAGATTTGCATGA+GGG - chr2.4:5066959-5066978 MS.gene85273:intron 40.0%
GCAATAGATTGTTGTCGAAC+TGG - chr2.4:5068025-5068044 MS.gene85273:intron 40.0%
GCTGCAGTATGATGTTGAAA+CGG + chr2.4:5067834-5067853 None:intergenic 40.0%
GCTGGTTAGTGAAGTTAAAG+AGG - chr2.4:5068225-5068244 MS.gene85273:CDS 40.0%
GTCACTTGAGTTCGATAACA+TGG - chr2.4:5066524-5066543 MS.gene85273:CDS 40.0%
TAAATAGGATCCCTGGATTC+GGG + chr2.4:5067418-5067437 None:intergenic 40.0%
TGAGTAACAATCCATGGTTG+AGG - chr2.4:5068062-5068081 MS.gene85273:CDS 40.0%
TGGATAGAAGGTCCTAAAGA+TGG - chr2.4:5067926-5067945 MS.gene85273:intron 40.0%
TGGTAGGAGGATCATATCTA+AGG + chr2.4:5067529-5067548 None:intergenic 40.0%
TTATCGAACTCAAGTGACTC+CGG + chr2.4:5066522-5066541 None:intergenic 40.0%
TTCGAGATTTGCACTCCATT+AGG + chr2.4:5067587-5067606 None:intergenic 40.0%
TTGCAGTTGTCACATCCAAT+TGG - chr2.4:5066622-5066641 MS.gene85273:CDS 40.0%
! AAACCTGCAATTGGTGGTTT+CGG + chr2.4:5067633-5067652 None:intergenic 40.0%
! AGTCATGAAGAATTGGCCTA+AGG - chr2.4:5066437-5066456 MS.gene85273:CDS 40.0%
! CCTAGTGGACCTAAATGAAA+AGG + chr2.4:5066369-5066388 None:intergenic 40.0%
! CCTTTTCATTTAGGTCCACT+AGG - chr2.4:5066366-5066385 MS.gene85273:CDS 40.0%
!! AGCTTGTTGGCTAAAGCTAT+GGG - chr2.4:5068115-5068134 MS.gene85273:CDS 40.0%
!! GAATGGGTTTTCCTTCAACA+TGG + chr2.4:5067148-5067167 None:intergenic 40.0%
!! GAGAAAGGAAAGCTTTGGAT+TGG - chr2.4:5068247-5068266 MS.gene85273:CDS 40.0%
!!! ACATGAGTTGTTTTGGTAGG+AGG + chr2.4:5067542-5067561 None:intergenic 40.0%
!!! TGAAAAAGGACAGTTTTGGG+TGG - chr2.4:5068003-5068022 MS.gene85273:intron 40.0%
AAATAGGATCCCTGGATTCG+GGG + chr2.4:5067417-5067436 None:intergenic 45.0%
AAGGACAACATGAGCAGACT+CGG - chr2.4:5066456-5066475 MS.gene85273:CDS 45.0%
AGAAGGCAAAGTGATGAAGC+TGG - chr2.4:5068207-5068226 MS.gene85273:CDS 45.0%
AGCTCATTCTTACTGGCAAG+CGG + chr2.4:5068099-5068118 None:intergenic 45.0%
AGGTCCTAAAGATGGAACAG+TGG - chr2.4:5067934-5067953 MS.gene85273:intron 45.0%
AGTCTGCTCATGTTGTCCTT+AGG + chr2.4:5066456-5066475 None:intergenic 45.0%
CTTACGTTGTCTGGAAATCC+TGG + chr2.4:5067977-5067996 None:intergenic 45.0%
CTTATGGTTGGACCAAATGG+AGG - chr2.4:5067101-5067120 MS.gene85273:CDS 45.0%
GCCAACAAGCTCATTCTTAC+TGG + chr2.4:5068106-5068125 None:intergenic 45.0%
GCCAGTAAGAATGAGCTTGT+TGG - chr2.4:5068102-5068121 MS.gene85273:CDS 45.0%
GCTTGTGTCAGTGAAGAAGA+TGG + chr2.4:5067207-5067226 None:intergenic 45.0%
GGACGGTCATCTCAATTCAA+CGG - chr2.4:5067366-5067385 MS.gene85273:intron 45.0%
GGACTGAGAAGATTTGCATG+AGG - chr2.4:5066958-5066977 MS.gene85273:intron 45.0%
GGCTATCAACAATCTGACTC+TGG + chr2.4:5067274-5067293 None:intergenic 45.0%
GGTTTCGGTAAAAAGGAGGA+AGG + chr2.4:5067618-5067637 None:intergenic 45.0%
TGGTGGTTTCGGTAAAAAGG+AGG + chr2.4:5067622-5067641 None:intergenic 45.0%
TTACCGAAACCACCAATTGC+AGG - chr2.4:5067627-5067646 MS.gene85273:CDS 45.0%
TTGTTGGAAGGAGAAGGAAC+AGG - chr2.4:5067497-5067516 MS.gene85273:CDS 45.0%
! AGTCCAATTTCGTGTCGCTA+AGG + chr2.4:5067806-5067825 None:intergenic 45.0%
! GCTTGTTGGCTAAAGCTATG+GGG - chr2.4:5068116-5068135 MS.gene85273:CDS 45.0%
!! GAGCTTGTTGGCTAAAGCTA+TGG - chr2.4:5068114-5068133 MS.gene85273:CDS 45.0%
ATGAGCAGACTCGGACTTCA+CGG - chr2.4:5066465-5066484 MS.gene85273:CDS 50.0%
CAAGTTGTTCCCCCATCCAT+CGG + chr2.4:5066590-5066609 None:intergenic 50.0%
CCATTGGCAACCCATGTTGA+AGG - chr2.4:5067134-5067153 MS.gene85273:CDS 50.0%
CGTTGTCTGGAAATCCTGGA+AGG + chr2.4:5067973-5067992 None:intergenic 50.0%
GATGGGGGAACAACTTGGTT+GGG - chr2.4:5066593-5066612 MS.gene85273:CDS 50.0%
GGTGCAACGTTGTGTTTCAC+AGG + chr2.4:5066408-5066427 None:intergenic 50.0%
TGGAAGGAGAAGGAACAGGT+AGG - chr2.4:5067501-5067520 MS.gene85273:CDS 50.0%
! AACACGACCATCGGCTAAAC+CGG + chr2.4:5066565-5066584 None:intergenic 50.0%
! ATGCATTGATCCCCGAATCC+AGG - chr2.4:5067404-5067423 MS.gene85273:intron 50.0%
! CATCGGCTAAACCGGTGTAA+GGG + chr2.4:5066557-5066576 None:intergenic 50.0%
! CCTTCAACATGGGTTGCCAA+TGG + chr2.4:5067137-5067156 None:intergenic 50.0%
! TGCATTGATCCCCGAATCCA+GGG - chr2.4:5067405-5067424 MS.gene85273:intron 50.0%
AAAACATGTGGGCGTCCCCT+TGG - chr2.4:5067020-5067039 MS.gene85273:intron 55.0%
CCATCGGACAACACGACCAT+CGG + chr2.4:5066574-5066593 None:intergenic 55.0%
CGATGGATGGGGGAACAACT+TGG - chr2.4:5066588-5066607 MS.gene85273:CDS 55.0%
CTAAACCGGTGTAAGGGCCA+CGG + chr2.4:5066551-5066570 None:intergenic 55.0%
GAATGCGTAGCTGACCTTCC+AGG - chr2.4:5067956-5067975 MS.gene85273:intron 55.0%
GAGTTCGATAACATGGGCCG+TGG - chr2.4:5066531-5066550 MS.gene85273:CDS 55.0%
GGATGGGGGAACAACTTGGT+TGG - chr2.4:5066592-5066611 MS.gene85273:CDS 55.0%
GGTCGTGTTGTCCGATGGAT+GGG - chr2.4:5066576-5066595 MS.gene85273:CDS 55.0%
GGTGTAGCCAGTCCTCCATT+TGG + chr2.4:5067116-5067135 None:intergenic 55.0%
GGTTGGACCAAATGGAGGAC+TGG - chr2.4:5067106-5067125 MS.gene85273:CDS 55.0%
GTCGTGTTGTCCGATGGATG+GGG - chr2.4:5066577-5066596 MS.gene85273:CDS 55.0%
TCGTGTTGTCCGATGGATGG+GGG - chr2.4:5066578-5066597 MS.gene85273:CDS 55.0%
TGGTCGTGTTGTCCGATGGA+TGG - chr2.4:5066575-5066594 MS.gene85273:CDS 55.0%
! CCATCGGCTAAACCGGTGTA+AGG + chr2.4:5066558-5066577 None:intergenic 55.0%
!! CCTTACACCGGTTTAGCCGA+TGG - chr2.4:5066555-5066574 MS.gene85273:CDS 55.0%
!! TGGAGGACTGGCTACACCAT+TGG - chr2.4:5067118-5067137 MS.gene85273:CDS 55.0%
CCGATGGTCGTGTTGTCCGA+TGG - chr2.4:5066571-5066590 MS.gene85273:CDS 60.0%
ATGGGCCGTGGCCCTTACAC+CGG - chr2.4:5066543-5066562 MS.gene85273:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 5066360 5068312 5066360 ID=MS.gene85273
chr2.4 mRNA 5066360 5068312 5066360 ID=MS.gene85273.t1;Parent=MS.gene85273
chr2.4 exon 5068029 5068312 5068029 ID=MS.gene85273.t1.exon1;Parent=MS.gene85273.t1
chr2.4 CDS 5068029 5068312 5068029 ID=cds.MS.gene85273.t1;Parent=MS.gene85273.t1
chr2.4 exon 5067429 5067713 5067429 ID=MS.gene85273.t1.exon2;Parent=MS.gene85273.t1
chr2.4 CDS 5067429 5067713 5067429 ID=cds.MS.gene85273.t1;Parent=MS.gene85273.t1
chr2.4 exon 5067024 5067191 5067024 ID=MS.gene85273.t1.exon3;Parent=MS.gene85273.t1
chr2.4 CDS 5067024 5067191 5067024 ID=cds.MS.gene85273.t1;Parent=MS.gene85273.t1
chr2.4 exon 5066360 5066759 5066360 ID=MS.gene85273.t1.exon4;Parent=MS.gene85273.t1
chr2.4 CDS 5066360 5066759 5066360 ID=cds.MS.gene85273.t1;Parent=MS.gene85273.t1
Gene Sequence

>MS.gene85273

ATGGACCCTTTTCATTTAGGTCCACTAGGAGAACATGAATTTAGACCTGTGAAACACAACGTTGCACCATATAAACAAGTCATGAAGAATTGGCCTAAGGACAACATGAGCAGACTCGGACTTCACGGAAAATTAGAATTCGAAAATGAAGTATTTGGACCGGAGTCACTTGAGTTCGATAACATGGGCCGTGGCCCTTACACCGGTTTAGCCGATGGTCGTGTTGTCCGATGGATGGGGGAACAACTTGGTTGGGAAACTTTTGCAGTTGTCACATCCAATTGGTGAGTATCATCAAGAAATTTGTAATCTAATTTGATTAAATCAGCTTTACAATACAACTCAAAATATGACATATAATTTTCATTCAACTATTATGATTCATGACTACGTGCCTATAGAGAGACACATTGCAATTATAATTGTTGAATGAAGATCAGACAATTCACATTTTGTGTTCGAAATATGAGATGTCTAAAATATGAAATGAAGATCTTCACGCAATTGCGTGATTGTATACACTCCATTAAGTCTAAAATATTATGTTTTTATATGTCCATATGTGCATTCATGCTAATGAAACTTGGTATTTTTAAAAGGACTGAGAAGATTTGCATGAGGGGAAATGATTCTACAACTCCAAAGCAATGGAAACATGAGAAAACATGTGGGCGTCCCCTTGGTTTAAGATTTGACAAAGAGAGTGGAGATTTATACATAGCAGATGCATATTATGGTCTTCTTATGGTTGGACCAAATGGAGGACTGGCTACACCATTGGCAACCCATGTTGAAGGAAAACCCATTCTCTTTGCAAATGATCTTGATATTCACAAGAACGGGTCCATCTTCTTCACTGACACAAGCACACGATACAATCGAGTGTAATTTCTTCTATTTCTATCTATTCACCAGAGTCAGATTGTTGATAGCCGTTGAATTGAGATCAAATGATATAGATTTAAAGTTCCGTATTTTATATTAAAAAAAATCTACAATAAAATTTGGACGGTCATCTCAATTCAACGGTTATCGATATGTTAAATGCATTGATCCCCGAATCCAGGGATCCTATTTATTTATTTCAGCTAAGTCCTTATGATGAGTGTTATTGTTTGCAGTGCACACTTTTTTATATTGTTGGAAGGAGAAGGAACAGGTAGGCTCCTTAGATATGATCCTCCTACCAAAACAACTCATGTTGTCTTGGATGGTCTTGTTTTTCCTAATGGAGTGCAAATCTCGAAAGATCAATCCTTCCTCCTTTTTACCGAAACCACCAATTGCAGGTTTTTAACTGCATTAATTATTTCTTTATCAACATACCGTACTTATTTTGCATTTTCTCTGCAACTTGTAATACTTGAGGTTAAATGGGATGCAACTCAATTGTAGTTACATTGTGATCTTTTATCTTGGTGCTGCAAACCATTTTTTAAAAAACCTTAGCGACACGAAATTGGACTCTCTACCGTTTCAACATCATACTGCAGCTATAATGAATCCAAATTCGTCCAACAAAATAACTAATTCATTAAATTCTTTAATGACAGGCTAATGAAACTTTGGATAGAAGGTCCTAAAGATGGAACAGTGGAATGCGTAGCTGACCTTCCAGGATTTCCAGACAACGTAAGAATGAATGAAAAAGGACAGTTTTGGGTGGCAATAGATTGTTGTCGAACTGGACCTCAAGAAGTCTTGAGTAACAATCCATGGTTGAGGAGTATTTACTTCCGCTTGCCAGTAAGAATGAGCTTGTTGGCTAAAGCTATGGGGATGAAAATGTACACAATGATTGCACTTCTTGATGATAACGGAAAGATTCTTGAAGTTCTTGAAGATCGAGAAGGCAAAGTGATGAAGCTGGTTAGTGAAGTTAAAGAGGAGAAAGGAAAGCTTTGGATTGGAACAGTAGCTCATAATCACATCTCCACCTTACCTTACCCTTAA
Protein sequence

>MS.gene85273.t1

MDPFHLGPLGEHEFRPVKHNVAPYKQVMKNWPKDNMSRLGLHGKLEFENEVFGPESLEFDNMGRGPYTGLADGRVVRWMGEQLGWETFAVVTSNWTEKICMRGNDSTTPKQWKHEKTCGRPLGLRFDKESGDLYIADAYYGLLMVGPNGGLATPLATHVEGKPILFANDLDIHKNGSIFFTDTSTRYNRVAHFFILLEGEGTGRLLRYDPPTKTTHVVLDGLVFPNGVQISKDQSFLLFTETTNCRLMKLWIEGPKDGTVECVADLPGFPDNVRMNEKGQFWVAIDCCRTGPQEVLSNNPWLRSIYFRLPVRMSLLAKAMGMKMYTMIALLDDNGKILEVLEDREGKVMKLVSEVKEEKGKLWIGTVAHNHISTLPYP