AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85485


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85485.t1 MTR_3g019090 93.194 191 13 0 1 191 1 191 1.72e-133 372
MS.gene85485.t1 MTR_3g019160 76.440 191 45 0 1 191 1 191 6.40e-108 307
MS.gene85485.t1 MTR_3g019120 77.320 194 37 2 1 189 1 192 4.19e-105 307
MS.gene85485.t1 MTR_3g019120 77.320 194 37 2 1 189 1 192 1.17e-103 307
MS.gene85485.t1 MTR_3g019150 72.251 191 52 1 1 191 1 190 1.37e-102 293
MS.gene85485.t1 MTR_8g039090 69.474 190 58 0 2 191 3 192 2.54e-101 290
MS.gene85485.t1 MTR_3g019090 95.070 142 7 0 50 191 1 142 1.28e-99 284
MS.gene85485.t1 MTR_3g019120 83.784 148 24 0 42 189 5 152 5.75e-88 266
MS.gene85485.t1 MTR_3g019150 71.287 101 29 0 91 191 34 134 6.55e-51 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85485.t1 AT3G27890 68.984 187 58 0 4 190 10 196 7.60e-92 266

Find 42 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTCTGCCTTGAAGTTTA+AGG 0.117582 3.4:+12585143 None:intergenic
AATCGACTATATTGATATTT+CGG 0.315855 3.4:-12585667 MS.gene85485:CDS
AAATATCAATATAGTCGATT+TGG 0.331349 3.4:+12585670 None:intergenic
ATTTCTGCCTTGAAGTTTAA+GGG 0.343293 3.4:+12585144 None:intergenic
AACATCATCTTCGCCAAATC+GGG 0.347815 3.4:-12585315 MS.gene85485:CDS
CATACTGGCCTCATTCGTTA+TGG 0.349761 3.4:-12586264 MS.gene85485:intron
CGCTAAAGAATGCGATTGAT+TGG 0.357043 3.4:-12585420 MS.gene85485:CDS
GGTTTACCAGCCCATGCATT+TGG 0.360432 3.4:+12585385 None:intergenic
ATTAAGATTGTTGCTCTCTC+TGG 0.362873 3.4:-12586309 MS.gene85485:CDS
CAACATCATCTTCGCCAAAT+CGG 0.377634 3.4:-12585316 MS.gene85485:CDS
GGCTCCTCCAACACTTACTA+TGG 0.391189 3.4:+12585357 None:intergenic
TATCAAGGAAAACCCCGATT+TGG 0.397239 3.4:+12585302 None:intergenic
CTGGATGCTTAAATACATCT+AGG 0.405336 3.4:+12585251 None:intergenic
CGAAAAGCTTCAACTTCAGC+TGG 0.413282 3.4:+12585600 None:intergenic
GAGACAGAATAATTATATTC+AGG 0.417383 3.4:+12585537 None:intergenic
GTAAGTGTTGGAGGAGCCTT+TGG 0.428342 3.4:-12585352 MS.gene85485:CDS
TTTAAGGGTAAATTCCTTCA+AGG 0.429920 3.4:+12585159 None:intergenic
TGAAGAGATCAAGAATAGAT+TGG 0.437949 3.4:-12585197 MS.gene85485:CDS
GAATATAATTATTCTGTCTC+AGG 0.442769 3.4:-12585534 MS.gene85485:intron
GAAAGTTCTTGTGTCCTTGA+AGG 0.447891 3.4:-12585173 MS.gene85485:CDS
TATTAATGAAATGAATATCA+AGG 0.449069 3.4:+12585287 None:intergenic
GCTAAAGAATGCGATTGATT+GGG 0.485585 3.4:-12585419 MS.gene85485:CDS
GAATTTACCCTTAAACTTCA+AGG 0.490730 3.4:-12585151 MS.gene85485:CDS
TTACCAGCCCATGCATTTGG+TGG 0.503471 3.4:+12585388 None:intergenic
AACTCTGATCTTGAGAAAGA+AGG 0.504804 3.4:-12585630 MS.gene85485:CDS
CAATCAATCGCATTCTTTAG+CGG 0.506871 3.4:+12585421 None:intergenic
GTTGGAGGAGCCTTTGGTGG+AGG 0.513008 3.4:-12585346 MS.gene85485:CDS
CATCAAGACCACCAAATGCA+TGG 0.519270 3.4:-12585396 MS.gene85485:CDS
GTAAATTACCATAACGAATG+AGG 0.533048 3.4:+12586256 None:intergenic
AGTGTTGGAGGAGCCTTTGG+TGG 0.533206 3.4:-12585349 MS.gene85485:CDS
TCAAGATCAGAGTTCAACAT+TGG 0.545865 3.4:+12585639 None:intergenic
GCTGCCATAGTAAGTGTTGG+AGG 0.554560 3.4:-12585361 MS.gene85485:CDS
AGACCACCAAATGCATGGGC+TGG 0.561726 3.4:-12585391 MS.gene85485:CDS
CCAACACTTACTATGGCAGC+AGG 0.570215 3.4:+12585364 None:intergenic
ATCAAGACCACCAAATGCAT+GGG 0.580578 3.4:-12585395 MS.gene85485:CDS
CCTGCTGCCATAGTAAGTGT+TGG 0.586831 3.4:-12585364 MS.gene85485:CDS
AATGAGGCCAGTATGAAAAG+AGG 0.609156 3.4:+12586272 None:intergenic
AATACATCTAGGTAGAACTC+AGG 0.627908 3.4:+12585262 None:intergenic
ACATCATCTTCGCCAAATCG+GGG 0.637712 3.4:-12585314 MS.gene85485:CDS
TTGATTAACAAATAATGACA+AGG 0.643119 3.4:-12586335 None:intergenic
ATGTAACGATGATGGTGACT+TGG 0.649079 3.4:-12585224 MS.gene85485:CDS
TGATTAACAAATAATGACAA+GGG 0.676333 3.4:-12586334 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTAATGAAATGAATATCA+AGG + chr3.4:12586178-12586197 None:intergenic 15.0%
!! TCTTTGAATATTAAATTTGA+TGG - chr3.4:12585704-12585723 MS.gene85485:CDS 15.0%
!!! CATTTTTTTTTTCTATTCAT+TGG - chr3.4:12585732-12585751 MS.gene85485:intron 15.0%
!! AAAAAGAATCTTATTGACAA+AGG + chr3.4:12585987-12586006 None:intergenic 20.0%
!! AAATATCAATATAGTCGATT+TGG + chr3.4:12585795-12585814 None:intergenic 20.0%
!! AATCGACTATATTGATATTT+CGG - chr3.4:12585795-12585814 MS.gene85485:intron 20.0%
!! ATATTGCATATTATATACAG+GGG - chr3.4:12585338-12585357 MS.gene85485:CDS 20.0%
!! CTACTTAGCAAAAAAAAAAA+AGG - chr3.4:12585427-12585446 MS.gene85485:intron 20.0%
!! GATATTGCATATTATATACA+GGG - chr3.4:12585337-12585356 MS.gene85485:CDS 20.0%
!!! ATATTTTAAATGTAGAGAAG+TGG + chr3.4:12585388-12585407 None:intergenic 20.0%
!!! GTTTAACTTGTTGAAAAAAT+AGG - chr3.4:12585629-12585648 MS.gene85485:CDS 20.0%
!!! TATTTTAAATGTAGAGAAGT+GGG + chr3.4:12585387-12585406 None:intergenic 20.0%
! AAAAGTTGAAATTAACCTGT+CGG - chr3.4:12585575-12585594 MS.gene85485:CDS 25.0%
! AAACTGAGTGAATATTGTTT+AGG - chr3.4:12585470-12585489 MS.gene85485:intron 25.0%
! AACTGAGTGAATATTGTTTA+GGG - chr3.4:12585471-12585490 MS.gene85485:intron 25.0%
! ATTCAAAGACTCAAAATCAA+AGG + chr3.4:12585693-12585712 None:intergenic 25.0%
! GAATATAATTATTCTGTCTC+AGG - chr3.4:12585928-12585947 MS.gene85485:intron 25.0%
! GAGACAGAATAATTATATTC+AGG + chr3.4:12585928-12585947 None:intergenic 25.0%
! GGATATTGCATATTATATAC+AGG - chr3.4:12585336-12585355 MS.gene85485:CDS 25.0%
! TATTGCATATTATATACAGG+GGG - chr3.4:12585339-12585358 MS.gene85485:CDS 25.0%
!! ATTTTAAATGTAGAGAAGTG+GGG + chr3.4:12585386-12585405 None:intergenic 25.0%
!! CTATTAAAACTCGCAATTTT+AGG - chr3.4:12585548-12585567 MS.gene85485:CDS 25.0%
!! TGAGTTTAGTTTAATTGGTA+GGG - chr3.4:12585315-12585334 MS.gene85485:CDS 25.0%
!!! AACTTGTTGAAAAAATAGGA+CGG - chr3.4:12585633-12585652 MS.gene85485:CDS 25.0%
!!! CTAAAATTGCGAGTTTTAAT+AGG + chr3.4:12585550-12585569 None:intergenic 25.0%
AAGGAAAAAAAGAAGAGAAG+AGG + chr3.4:12585260-12585279 None:intergenic 30.0%
AATTTCTGCCTTGAAGTTTA+AGG + chr3.4:12586322-12586341 None:intergenic 30.0%
ACCAATTAAACTAAACTCAC+CGG + chr3.4:12585314-12585333 None:intergenic 30.0%
ACTGAGTGAATATTGTTTAG+GGG - chr3.4:12585472-12585491 MS.gene85485:intron 30.0%
ATATTATATACAGGGGGTTA+GGG - chr3.4:12585345-12585364 MS.gene85485:CDS 30.0%
ATTTCTGCCTTGAAGTTTAA+GGG + chr3.4:12586321-12586340 None:intergenic 30.0%
CTAAGCAAAAATGTTCTTGA+TGG - chr3.4:12585766-12585785 MS.gene85485:intron 30.0%
GAATTTACCCTTAAACTTCA+AGG - chr3.4:12586311-12586330 MS.gene85485:CDS 30.0%
GTAAATTACCATAACGAATG+AGG + chr3.4:12585209-12585228 None:intergenic 30.0%
TGAAGAGATCAAGAATAGAT+TGG - chr3.4:12586265-12586284 MS.gene85485:CDS 30.0%
TTTAAGGGTAAATTCCTTCA+AGG + chr3.4:12586306-12586325 None:intergenic 30.0%
! GTGAGTTTAGTTTAATTGGT+AGG - chr3.4:12585314-12585333 MS.gene85485:CDS 30.0%
! TTTTCAACAAGTTAAACGAC+TGG + chr3.4:12585626-12585645 None:intergenic 30.0%
!! AATTGCGAGTTTTAATAGGT+CGG + chr3.4:12585546-12585565 None:intergenic 30.0%
!! TAAAACTCGCAATTTTAGGT+TGG - chr3.4:12585552-12585571 MS.gene85485:CDS 30.0%
AACTCTGATCTTGAGAAAGA+AGG - chr3.4:12585832-12585851 MS.gene85485:intron 35.0%
AATACATCTAGGTAGAACTC+AGG + chr3.4:12586203-12586222 None:intergenic 35.0%
ATTAAGATTGTTGCTCTCTC+TGG - chr3.4:12585153-12585172 MS.gene85485:CDS 35.0%
CAATCAATCGCATTCTTTAG+CGG + chr3.4:12586044-12586063 None:intergenic 35.0%
CATATTATATACAGGGGGTT+AGG - chr3.4:12585344-12585363 MS.gene85485:CDS 35.0%
CCAATTAAACTAAACTCACC+GGG + chr3.4:12585313-12585332 None:intergenic 35.0%
CCAGAAAAATGTAACGATGA+TGG - chr3.4:12586230-12586249 MS.gene85485:intron 35.0%
CTCGAAGTAGTCATTTAGAA+AGG + chr3.4:12585279-12585298 None:intergenic 35.0%
CTGAGTGAATATTGTTTAGG+GGG - chr3.4:12585473-12585492 MS.gene85485:intron 35.0%
CTGGATGCTTAAATACATCT+AGG + chr3.4:12586214-12586233 None:intergenic 35.0%
CTTTCTAAATGACTACTTCG+AGG - chr3.4:12585277-12585296 MS.gene85485:CDS 35.0%
GCTAAAGAATGCGATTGATT+GGG - chr3.4:12586043-12586062 MS.gene85485:intron 35.0%
GTTTAATAAACCTATCCGAC+AGG + chr3.4:12585593-12585612 None:intergenic 35.0%
TCAAGATCAGAGTTCAACAT+TGG + chr3.4:12585826-12585845 None:intergenic 35.0%
TTGAAATTAACCTGTCGGAT+AGG - chr3.4:12585580-12585599 MS.gene85485:CDS 35.0%
TTGTTGAAAAAATAGGACGG+TGG - chr3.4:12585636-12585655 MS.gene85485:CDS 35.0%
! CCATCATCGTTACATTTTTC+TGG + chr3.4:12586233-12586252 None:intergenic 35.0%
AACATCATCTTCGCCAAATC+GGG - chr3.4:12586147-12586166 MS.gene85485:intron 40.0%
AATGAGGCCAGTATGAAAAG+AGG + chr3.4:12585193-12585212 None:intergenic 40.0%
ATCAAGACCACCAAATGCAT+GGG - chr3.4:12586067-12586086 MS.gene85485:intron 40.0%
CAACATCATCTTCGCCAAAT+CGG - chr3.4:12586146-12586165 MS.gene85485:intron 40.0%
CGCTAAAGAATGCGATTGAT+TGG - chr3.4:12586042-12586061 MS.gene85485:intron 40.0%
GAAAGTTCTTGTGTCCTTGA+AGG - chr3.4:12586289-12586308 MS.gene85485:CDS 40.0%
TATCAAGGAAAACCCCGATT+TGG + chr3.4:12586163-12586182 None:intergenic 40.0%
! ATGTAACGATGATGGTGACT+TGG - chr3.4:12586238-12586257 MS.gene85485:intron 40.0%
! CCCGGTGAGTTTAGTTTAAT+TGG - chr3.4:12585310-12585329 MS.gene85485:CDS 40.0%
! CGAAAAGCCTCTTTTCATAC+TGG - chr3.4:12585183-12585202 MS.gene85485:CDS 40.0%
ACATCATCTTCGCCAAATCG+GGG - chr3.4:12586148-12586167 MS.gene85485:intron 45.0%
CATCAAGACCACCAAATGCA+TGG - chr3.4:12586066-12586085 MS.gene85485:intron 45.0%
CGAAAAGCTTCAACTTCAGC+TGG + chr3.4:12585865-12585884 None:intergenic 45.0%
CTAAGTAGGGGTGGCAAAAT+AGG - chr3.4:12585506-12585525 MS.gene85485:intron 45.0%
CTTCGAGGCTAATCAAATCC+CGG - chr3.4:12585292-12585311 MS.gene85485:CDS 45.0%
GGAGAGAAGACACTAAGTAG+GGG - chr3.4:12585494-12585513 MS.gene85485:intron 45.0%
GGGAGAGAAGACACTAAGTA+GGG - chr3.4:12585493-12585512 MS.gene85485:intron 45.0%
TGAAAAAATAGGACGGTGGC+AGG - chr3.4:12585640-12585659 MS.gene85485:CDS 45.0%
! CATACTGGCCTCATTCGTTA+TGG - chr3.4:12585198-12585217 MS.gene85485:CDS 45.0%
! TTGAGCTTTTCCTCCACCAA+AGG + chr3.4:12586129-12586148 None:intergenic 45.0%
CAAAATAGGTCGAGCCTGTC+GGG - chr3.4:12585520-12585539 MS.gene85485:intron 50.0%
CCAACACTTACTATGGCAGC+AGG + chr3.4:12586101-12586120 None:intergenic 50.0%
CCTGCTGCCATAGTAAGTGT+TGG - chr3.4:12586098-12586117 MS.gene85485:intron 50.0%
GAGAAGACACTAAGTAGGGG+TGG - chr3.4:12585497-12585516 MS.gene85485:intron 50.0%
GCAAAATAGGTCGAGCCTGT+CGG - chr3.4:12585519-12585538 MS.gene85485:intron 50.0%
GGCTCCTCCAACACTTACTA+TGG + chr3.4:12586108-12586127 None:intergenic 50.0%
GGGGAGAGAAGACACTAAGT+AGG - chr3.4:12585492-12585511 MS.gene85485:intron 50.0%
GGTTTACCAGCCCATGCATT+TGG + chr3.4:12586080-12586099 None:intergenic 50.0%
TTACCAGCCCATGCATTTGG+TGG + chr3.4:12586077-12586096 None:intergenic 50.0%
! TTTTAATAGGTCGGCCCGAC+AGG + chr3.4:12585537-12585556 None:intergenic 50.0%
!! GCTGCCATAGTAAGTGTTGG+AGG - chr3.4:12586101-12586120 MS.gene85485:intron 50.0%
!! GTAAGTGTTGGAGGAGCCTT+TGG - chr3.4:12586110-12586129 MS.gene85485:intron 50.0%
AGACCACCAAATGCATGGGC+TGG - chr3.4:12586071-12586090 MS.gene85485:intron 55.0%
! AGTGTTGGAGGAGCCTTTGG+TGG - chr3.4:12586113-12586132 MS.gene85485:intron 55.0%
GGGGGTTAGGGTTCGAAGCT+CGG - chr3.4:12585357-12585376 MS.gene85485:CDS 60.0%
GTTGGAGGAGCCTTTGGTGG+AGG - chr3.4:12586116-12586135 MS.gene85485:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 12585141 12586343 12585141 ID=MS.gene85485
chr3.4 mRNA 12585141 12586343 12585141 ID=MS.gene85485.t1;Parent=MS.gene85485
chr3.4 exon 12586265 12586343 12586265 ID=MS.gene85485.t1.exon1;Parent=MS.gene85485.t1
chr3.4 CDS 12586265 12586343 12586265 ID=cds.MS.gene85485.t1;Parent=MS.gene85485.t1
chr3.4 exon 12585535 12585726 12585535 ID=MS.gene85485.t1.exon2;Parent=MS.gene85485.t1
chr3.4 CDS 12585535 12585726 12585535 ID=cds.MS.gene85485.t1;Parent=MS.gene85485.t1
chr3.4 exon 12585141 12585445 12585141 ID=MS.gene85485.t1.exon3;Parent=MS.gene85485.t1
chr3.4 CDS 12585141 12585445 12585141 ID=cds.MS.gene85485.t1;Parent=MS.gene85485.t1
Gene Sequence

>MS.gene85485

ATGACAAGGGTGATTAAGATTGTTGCTCTCTCTGGTTCTCTTCGAAAAGCCTCTTTTCATACTGGCCTCATTCGTTATGGTAATTTACTTCTTATTCAAACACTAATTCTTCTCTTCCTCTTCTCTTCTTTTTTTCCTTTCTAAATGACTACTTCGAGGCTAATCAAATCCCGGTGAGTTTAGTTTAATTGGTAGGGATATTGCATATTATATACAGGGGGTTAGGGTTCGAAGCTCGGACACCCCACTTCTCTACATTTAAAATATGTGAAATTTAGCAAATAGACTACTTAGCAAAAAAAAAAAAGGCAAAATGTAAAATAAAAAATAAACTGAGTGAATATTGTTTAGGGGGAGAGAAGACACTAAGTAGGGGTGGCAAAATAGGTCGAGCCTGTCGGGCCGACCTATTAAAACTCGCAATTTTAGGTTGGAAAAGTTGAAATTAACCTGTCGGATAGGTTTATTAAACTCAAAATTAACCAGTCGTTTAACTTGTTGAAAAAATAGGACGGTGGCAGGACGATTTGACATAAGTAGATGAATTAACCTTTGATTTTGAGTCTTTGAATATTAAATTTGATGGTTTTACATTTTTTTTTTCTATTCATTGGTAGCTATTGAGCTAAGCAAAAATGTTCTTGATGGTATCCAAATCGACTATATTGATATTTCGGACTTGCCAATGTTGAACTCTGATCTTGAGAAAGAAGGAACTTTTCCAGCTGAAGTTGAAGCTTTTCGTCAAAAGATTCTTGCAGCTGATAGTGTTCTCTTTGCTTCTCCTGAATATAATTATTCTGTCTCAGGTAAGTAAACATCATGTTGTTTTTTTCTTTATAACCTTTGTCAATAAGATTCTTTTTGCTAATTTATTATTTTCTCGCATTTTTTTCAGCTCCGCTAAAGAATGCGATTGATTGGGCATCAAGACCACCAAATGCATGGGCTGGTAAACCTGCTGCCATAGTAAGTGTTGGAGGAGCCTTTGGTGGAGGAAAAGCTCAACATCATCTTCGCCAAATCGGGGTTTTCCTTGATATTCATTTCATTAATAAACCTGAGTTCTACCTAGATGTATTTAAGCATCCAGAAAAATGTAACGATGATGGTGACTTGGTTGATGAAGAGATCAAGAATAGATTGGAGAAAGTTCTTGTGTCCTTGAAGGAATTTACCCTTAAACTTCAAGGCAGAAATTGA

Protein sequence

>MS.gene85485.t1

MTRVIKIVALSGSLRKASFHTGLIRYAIELSKNVLDGIQIDYIDISDLPMLNSDLEKEGTFPAEVEAFRQKILAADSVLFASPEYNYSVSAPLKNAIDWASRPPNAWAGKPAAIVSVGGAFGGGKAQHHLRQIGVFLDIHFINKPEFYLDVFKHPEKCNDDGDLVDEEIKNRLEKVLVSLKEFTLKLQGRN