AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene86987


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86987.t1 MTR_2g104810 88.119 101 12 0 40 140 19 119 2.75e-62 190
MS.gene86987.t1 MTR_4g116860 49.451 91 35 1 107 197 1542 1621 4.64e-18 82.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene86987.t1 AT1G80650 45.652 92 40 1 103 194 111 192 2.99e-18 79.3
MS.gene86987.t1 AT4G00420 44.330 97 43 3 103 199 59 144 2.29e-16 73.6
MS.gene86987.t1 AT4G00420 44.330 97 43 3 103 199 80 165 3.64e-16 73.6
MS.gene86987.t1 AT4G00420 44.330 97 43 3 103 199 81 166 3.71e-16 73.6
MS.gene86987.t1 AT4G00420 44.330 97 43 3 103 199 81 166 3.71e-16 73.6
MS.gene86987.t1 AT4G00420 44.330 97 43 3 103 199 81 166 3.71e-16 73.6
MS.gene86987.t1 AT5G20320 39.823 113 57 1 85 197 1586 1687 6.84e-15 73.2
MS.gene86987.t1 AT5G20320 39.823 113 57 1 85 197 1375 1476 7.29e-15 72.8
MS.gene86987.t1 AT5G20320 39.823 113 57 1 85 197 1600 1701 7.32e-15 72.8
MS.gene86987.t1 AT1G80650 39.130 92 36 1 103 194 111 182 9.35e-12 61.6

Find 24 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTAAAATGACACTCTCTT+TGG 0.292490 2.3:-205196 None:intergenic
CTTGAATAATCAAATGAAAA+TGG 0.300650 2.3:+205098 MS.gene86987:CDS
ATTCAAGGTTATTGTTGAAA+TGG 0.336999 2.3:+206356 MS.gene86987:CDS
TTCATTTGATTATTCAAGTT+GGG 0.380029 2.3:-205093 None:intergenic
TTCTTCTCACTCCACCTTCA+AGG 0.388623 2.3:+204922 MS.gene86987:CDS
TGTTTGTTAGGTATGAAGAA+AGG 0.445413 2.3:+205344 MS.gene86987:intron
TTGTGGTACTTAAAGCATAT+AGG 0.446945 2.3:+206498 MS.gene86987:CDS
GTTTGTTAGGTATGAAGAAA+GGG 0.447288 2.3:+205345 MS.gene86987:intron
TTTCATTTGATTATTCAAGT+TGG 0.449161 2.3:-205094 None:intergenic
TGGAAGATGAAACTGAAGAT+TGG 0.449737 2.3:-205176 None:intergenic
GTTATTGTTGAAATGGAGAC+AGG 0.475716 2.3:+206363 MS.gene86987:CDS
AGACATGTGCTGTAAATCAT+TGG 0.504933 2.3:+205390 MS.gene86987:CDS
GGCAGCAGACAATGCAGCCG+AGG 0.547846 2.3:+206470 MS.gene86987:CDS
TAAGTACCACAATGCACCCT+CGG 0.553491 2.3:-206487 None:intergenic
AAGACAACAACTTCAGATGA+AGG 0.557587 2.3:+205219 MS.gene86987:CDS
TTAGGTATGAAGAAAGGGTG+TGG 0.557962 2.3:+205350 MS.gene86987:intron
TGTGTTTGAGTGTTGTAAAG+AGG 0.558213 2.3:+205421 MS.gene86987:CDS
GCAGCAGACAATGCAGCCGA+GGG 0.565055 2.3:+206471 MS.gene86987:CDS
TCTTTACAACACTCAAACAC+AGG 0.578805 2.3:-205419 None:intergenic
ATTATACACAATACAAAACA+TGG 0.645969 2.3:+204886 MS.gene86987:CDS
TTTGAGTGTTGTAAAGAGGA+AGG 0.648639 2.3:+205425 MS.gene86987:CDS
TTACAACACTCAAACACAGG+AGG 0.658087 2.3:-205416 None:intergenic
ACTTACATTATGCAATGACA+TGG 0.673466 2.3:-205449 None:intergenic
ATGCAGCCGAGGGTGCATTG+TGG 0.682343 2.3:+206481 MS.gene86987:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAATTAAAATAAATAATT+GGG + chr2.3:204969-204988 MS.gene86987:intron 0.0%
!!! TTTTAATTAAAATAAATAAT+TGG + chr2.3:204968-204987 MS.gene86987:intron 0.0%
!!! TTTTATTTATGAAAAATGAA+GGG + chr2.3:205474-205493 MS.gene86987:intron 10.0%
!!! TTTTTATTTATGAAAAATGA+AGG + chr2.3:205473-205492 MS.gene86987:intron 10.0%
!! AAAAAAAATTATTACCTTGA+AGG - chr2.3:204939-204958 None:intergenic 15.0%
!!! ACAAAAAAAATGAACTTTTT+TGG - chr2.3:205274-205293 None:intergenic 15.0%
!! ATATAAACAACTTACAACTT+AGG + chr2.3:205713-205732 MS.gene86987:intron 20.0%
!! ATTATACACAATACAAAACA+TGG + chr2.3:204886-204905 MS.gene86987:CDS 20.0%
!! CTTGAATAATCAAATGAAAA+TGG + chr2.3:205098-205117 MS.gene86987:CDS 20.0%
!! TCATTATGAATTGTAGAAAA+GGG - chr2.3:206266-206285 None:intergenic 20.0%
!! TTCATTATGAATTGTAGAAA+AGG - chr2.3:206267-206286 None:intergenic 20.0%
!! TTTCATTTGATTATTCAAGT+TGG - chr2.3:205097-205116 None:intergenic 20.0%
!!! AATGAGAAAAATGAGTATTT+TGG + chr2.3:205315-205334 MS.gene86987:intron 20.0%
!!! GAAATGAAATTTTCTATAAG+TGG + chr2.3:205522-205541 MS.gene86987:intron 20.0%
!!! TTCATTTGATTATTCAAGTT+GGG - chr2.3:205096-205115 None:intergenic 20.0%
!!! TTTTGGTATTATTGTTTGTT+AGG + chr2.3:205332-205351 MS.gene86987:intron 20.0%
! AAAAACATTGTAGAGGTTTA+TGG + chr2.3:206423-206442 MS.gene86987:CDS 25.0%
! AAAAATTATTACCTTGAAGG+TGG - chr2.3:204936-204955 None:intergenic 25.0%
! AAAAGTTTATGCTGCAAAAA+TGG + chr2.3:205065-205084 MS.gene86987:CDS 25.0%
! AAAGGGAAGCAAAATATAAT+AGG - chr2.3:206249-206268 None:intergenic 25.0%
! AATATTGCAAAAGATTTAGC+TGG - chr2.3:206139-206158 None:intergenic 25.0%
! AATGAAGGGAAAAAAAACTA+TGG + chr2.3:205488-205507 MS.gene86987:intron 25.0%
! ATTCAAGGTTATTGTTGAAA+TGG + chr2.3:206356-206375 MS.gene86987:CDS 25.0%
! GGAAAACATGCAATAATTAA+TGG - chr2.3:205253-205272 None:intergenic 25.0%
! TCATGCAATAAGACAAATAT+TGG - chr2.3:206222-206241 None:intergenic 25.0%
! TCCTCATCAAAAAAAGAAAA+TGG + chr2.3:206449-206468 MS.gene86987:CDS 25.0%
! TCTACAATTCATAATGAAAC+TGG + chr2.3:206269-206288 MS.gene86987:intron 25.0%
! TCTCAAATTATTAACAGTGT+CGG + chr2.3:205902-205921 MS.gene86987:intron 25.0%
!! ATAAACCTCTACAATGTTTT+TGG - chr2.3:206424-206443 None:intergenic 25.0%
AACCTGTGAAAAACATCAAT+TGG - chr2.3:206333-206352 None:intergenic 30.0%
AAGATTATTCCATTGCATGT+TGG + chr2.3:206110-206129 MS.gene86987:intron 30.0%
ACAATTCATAATGAAACTGG+AGG + chr2.3:206272-206291 MS.gene86987:intron 30.0%
ACTTACATTATGCAATGACA+TGG - chr2.3:205452-205471 None:intergenic 30.0%
AGTAACCAAAAACATTGTAG+AGG + chr2.3:206416-206435 MS.gene86987:CDS 30.0%
TTAGAAGATGCATAGATTAG+TGG + chr2.3:205747-205766 MS.gene86987:intron 30.0%
TTGTGGTACTTAAAGCATAT+AGG + chr2.3:206498-206517 MS.gene86987:CDS 30.0%
! AAGTGGTATATTTTGTGTGT+TGG + chr2.3:205539-205558 MS.gene86987:intron 30.0%
! GACAAATATTGGTTGATATC+TGG - chr2.3:206211-206230 None:intergenic 30.0%
! GTTTGTTAGGTATGAAGAAA+GGG + chr2.3:205345-205364 MS.gene86987:intron 30.0%
! TGTTTGTTAGGTATGAAGAA+AGG + chr2.3:205344-205363 MS.gene86987:intron 30.0%
! TTGCAGCATAAACTTTTGTT+CGG - chr2.3:205062-205081 None:intergenic 30.0%
! TTTTCACAGGTTTACATTCA+AGG + chr2.3:206341-206360 MS.gene86987:intron 30.0%
!!! GCCATTTTCTTTTTTTGATG+AGG - chr2.3:206453-206472 None:intergenic 30.0%
AAGACAACAACTTCAGATGA+AGG + chr2.3:205219-205238 MS.gene86987:CDS 35.0%
AGACATGTGCTGTAAATCAT+TGG + chr2.3:205390-205409 MS.gene86987:CDS 35.0%
GTTATTGTTGAAATGGAGAC+AGG + chr2.3:206363-206382 MS.gene86987:CDS 35.0%
TCAGCTGTAGATTTCAAATC+AGG + chr2.3:205683-205702 MS.gene86987:intron 35.0%
TCTTTACAACACTCAAACAC+AGG - chr2.3:205422-205441 None:intergenic 35.0%
TGGAAGATGAAACTGAAGAT+TGG - chr2.3:205179-205198 None:intergenic 35.0%
! GACCAATTGATGTTTTTCAC+AGG + chr2.3:206328-206347 MS.gene86987:intron 35.0%
! TGTGTTTGAGTGTTGTAAAG+AGG + chr2.3:205421-205440 MS.gene86987:CDS 35.0%
! TTTGAGTGTTGTAAAGAGGA+AGG + chr2.3:205425-205444 MS.gene86987:CDS 35.0%
! TTTTTCGCTGTGCGATTATA+GGG + chr2.3:205637-205656 MS.gene86987:intron 35.0%
!!! CCAAAGAGAGTGTCATTTTA+GGG + chr2.3:205196-205215 MS.gene86987:CDS 35.0%
!!! GCATCAATGTTTTTCAAGAC+TGG - chr2.3:205153-205172 None:intergenic 35.0%
!!! TCCAAAGAGAGTGTCATTTT+AGG + chr2.3:205195-205214 MS.gene86987:CDS 35.0%
!!! TTTTTTCGCTGTGCGATTAT+AGG + chr2.3:205636-205655 MS.gene86987:intron 35.0%
CCCTAAAATGACACTCTCTT+TGG - chr2.3:205199-205218 None:intergenic 40.0%
GATGTTAGAGCCTAGTGTTT+CGG - chr2.3:206058-206077 None:intergenic 40.0%
TATATGTGTCAGTGTCGTGT+CGG - chr2.3:205860-205879 None:intergenic 40.0%
TATGTGCTTGAGTGCTTCAT+AGG - chr2.3:205788-205807 None:intergenic 40.0%
TTACAACACTCAAACACAGG+AGG - chr2.3:205419-205438 None:intergenic 40.0%
TTAGGTATGAAGAAAGGGTG+TGG + chr2.3:205350-205369 MS.gene86987:intron 40.0%
! GACGTTTTATGCAGGTATCT+GGG - chr2.3:206029-206048 None:intergenic 40.0%
ACTCAAGCACATACACTCCT+CGG + chr2.3:205795-205814 MS.gene86987:intron 45.0%
AGCTGGATACCAACATGCAA+TGG - chr2.3:206122-206141 None:intergenic 45.0%
ATGAAGCACGGATATGGACA+CGG - chr2.3:205957-205976 None:intergenic 45.0%
CTGTGCGATTATAGGGAAAC+TGG + chr2.3:205644-205663 MS.gene86987:intron 45.0%
TAAGTACCACAATGCACCCT+CGG - chr2.3:206490-206509 None:intergenic 45.0%
TGAGTGCTTCATAGGTGTGT+GGG - chr2.3:205780-205799 None:intergenic 45.0%
TTCATAGCCACACACAGACA+CGG + chr2.3:205972-205991 MS.gene86987:intron 45.0%
TTCTTCTCACTCCACCTTCA+AGG + chr2.3:204922-204941 MS.gene86987:CDS 45.0%
TTGAGTGCTTCATAGGTGTG+TGG - chr2.3:205781-205800 None:intergenic 45.0%
! GGACGTTTTATGCAGGTATC+TGG - chr2.3:206030-206049 None:intergenic 45.0%
!!! TTGTTTTTTTTTTTTTAAAT+TGG + chr2.3:205003-205022 MS.gene86987:intron 5.0%
ACGGATATGGACACGGAAAC+CGG - chr2.3:205950-205969 None:intergenic 50.0%
CTGTGTGTGGCTATGAAGCA+CGG - chr2.3:205969-205988 None:intergenic 50.0%
GCACATACACTCCTCGGATT+AGG + chr2.3:205801-205820 MS.gene86987:intron 50.0%
GTGGCTATGAAGCACGGATA+TGG - chr2.3:205963-205982 None:intergenic 50.0%
TTCATAGGTGTGTGGGCACT+AGG - chr2.3:205773-205792 None:intergenic 50.0%
! GGAGTGTGGACGTTTTATGC+AGG - chr2.3:206037-206056 None:intergenic 50.0%
AGGTATCTGGGCTTGATCCC+AGG - chr2.3:206017-206036 None:intergenic 55.0%
CGTCCACACTCCGAAACACT+AGG + chr2.3:206045-206064 MS.gene86987:intron 55.0%
GAGCCTAGTGTTTCGGAGTG+TGG - chr2.3:206051-206070 None:intergenic 55.0%
GTCAGTATGCGTGTCGTGTC+CGG + chr2.3:205928-205947 MS.gene86987:intron 55.0%
! TGTCAGTGTCGTGTCGGTGT+TGG - chr2.3:205854-205873 None:intergenic 55.0%
ACACGACACCTGTCCGACAC+CGG - chr2.3:205831-205850 None:intergenic 60.0%
ACAGACACGGAGAGGGGGAA+GGG + chr2.3:205985-206004 MS.gene86987:intron 60.0%
AGCCACACACAGACACGGAG+AGG + chr2.3:205977-205996 MS.gene86987:intron 60.0%
AGGGGGAAGGGTGGAAACCT+GGG + chr2.3:205997-206016 MS.gene86987:intron 60.0%
CACACACAGACACGGAGAGG+GGG + chr2.3:205980-205999 MS.gene86987:intron 60.0%
CCACACACAGACACGGAGAG+GGG + chr2.3:205979-205998 MS.gene86987:intron 60.0%
GCAGCAGACAATGCAGCCGA+GGG + chr2.3:206471-206490 MS.gene86987:CDS 60.0%
GCCACACACAGACACGGAGA+GGG + chr2.3:205978-205997 MS.gene86987:intron 60.0%
! ACTCCTCGGATTAGGCGTCC+CGG + chr2.3:205809-205828 MS.gene86987:intron 60.0%
! ATGCAGCCGAGGGTGCATTG+TGG + chr2.3:206481-206500 MS.gene86987:CDS 60.0%
ACACCGGGACGCCTAATCCG+AGG - chr2.3:205815-205834 None:intergenic 65.0%
CACAGACACGGAGAGGGGGA+AGG + chr2.3:205984-206003 MS.gene86987:intron 65.0%
CACGACACCTGTCCGACACC+GGG - chr2.3:205830-205849 None:intergenic 65.0%
CCCCTCTCCGTGTCTGTGTG+TGG - chr2.3:205982-206001 None:intergenic 65.0%
GAGGGGGAAGGGTGGAAACC+TGG + chr2.3:205996-206015 MS.gene86987:intron 65.0%
GGCAGCAGACAATGCAGCCG+AGG + chr2.3:206470-206489 MS.gene86987:CDS 65.0%
! CGGATTAGGCGTCCCGGTGT+CGG + chr2.3:205815-205834 MS.gene86987:intron 65.0%
GACACGGAGAGGGGGAAGGG+TGG + chr2.3:205988-206007 MS.gene86987:intron 70.0%
! TAGGCGTCCCGGTGTCGGAC+AGG + chr2.3:205820-205839 MS.gene86987:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 204831 206542 204831 ID=MS.gene86987
chr2.3 mRNA 204831 206542 204831 ID=MS.gene86987.t1;Parent=MS.gene86987
chr2.3 exon 204831 204943 204831 ID=MS.gene86987.t1.exon1;Parent=MS.gene86987.t1
chr2.3 CDS 204831 204943 204831 ID=cds.MS.gene86987.t1;Parent=MS.gene86987.t1
chr2.3 exon 205053 205240 205053 ID=MS.gene86987.t1.exon2;Parent=MS.gene86987.t1
chr2.3 CDS 205053 205240 205053 ID=cds.MS.gene86987.t1;Parent=MS.gene86987.t1
chr2.3 exon 205354 205465 205354 ID=MS.gene86987.t1.exon3;Parent=MS.gene86987.t1
chr2.3 CDS 205354 205465 205354 ID=cds.MS.gene86987.t1;Parent=MS.gene86987.t1
chr2.3 exon 206350 206542 206350 ID=MS.gene86987.t1.exon4;Parent=MS.gene86987.t1
chr2.3 CDS 206350 206542 206350 ID=cds.MS.gene86987.t1;Parent=MS.gene86987.t1
Gene Sequence

>MS.gene86987

ATGAAATTTCAACTATTTCATTCTGTTATTTGCACAGCTGAAGTCTGTTTACAGCATTATACACAATACAAAACATGGACAAGAGCAATAATTCTTCTCACTCCACCTTCAAGGTAATAATTTTTTTTCTTCATTTTTTTTAATTAAAATAAATAATTGGGTATATGAAAAATTGTTTTTTTTTTTTTAAATTGGTTGAATTGAAATTCTTATCTTTTGCAGAGTAAACCGAACAAAAGTTTATGCTGCAAAAATGGTGAAACCCAACTTGAATAATCAAATGAAAATGGTTGAAACTGTTGAAAGCATTGTTCAAAATCCAGTCTTGAAAAACATTGATGCTGTCCAATCTTCAGTTTCATCTTCCAAAGAGAGTGTCATTTTAGGGAAGACAACAACTTCAGATGAAGGTAAACTAACCATTAATTATTGCATGTTTTCCAAAAAAGTTCATTTTTTTTGTATTTTTTTTCTTTCTTGTAAAAATGAGAAAAATGAGTATTTTGGTATTATTGTTTGTTAGGTATGAAGAAAGGGTGTGGTAGATCAAAGCTGTATGAGACATGTGCTGTAAATCATTGGAAGCCTCCTGTGTTTGAGTGTTGTAAAGAGGAAGGACCATGTCATTGCATAATGTAAGTCTTTTTATTTATGAAAAATGAAGGGAAAAAAAACTATGGTTATGATGATTGAAATGAAATTTTCTATAAGTGGTATATTTTGTGTGTTGGTAGCTGCAAGTTTATATAAATCAGAATCAATATAGCAAATAAGTGACATTTTCTATTTTGATTTATTCTTTTATTTTTTTCGCTGTGCGATTATAGGGAAACTGGCTTCTATGTTGATTTCTCAGCTGTAGATTTCAAATCAGGATTTTGTATATAAACAACTTACAACTTAGGTGCATAAGTTCTTAGAAGATGCATAGATTAGTGGCCTAGTGCCCACACACCTATGAAGCACTCAAGCACATACACTCCTCGGATTAGGCGTCCCGGTGTCGGACAGGTGTCGTGTCCAACACCGACACGACACTGACACATATAATTACACTAAATTATGTGATTTTCTCAAATTATTAACAGTGTCGGCATGTCAGTATGCGTGTCGTGTCCGGTTTCCGTGTCCATATCCGTGCTTCATAGCCACACACAGACACGGAGAGGGGGAAGGGTGGAAACCTGGGATCAAGCCCAGATACCTGCATAAAACGTCCACACTCCGAAACACTAGGCTCTAACATCTTGTAGATTTATTTCTGTTCATTCTTGTTCACAAGATTATTCCATTGCATGTTGGTATCCAGCTAAATCTTTTGCAATATTAGTCTTAATATAACGATTTATTGCTATGAAAAGTTGTTGAATTTCTTATCCAGATATCAACCAATATTTGTCTTATTGCATGACTTTCCTATTATATTTTGCTTCCCTTTTCTACAATTCATAATGAAACTGGAGGAAATACAGTCATAACTTTTTTCTTGTTCTCAGTGACCAATTGATGTTTTTCACAGGTTTACATTCAAGGTTATTGTTGAAATGGAGACAGGTAAAGCTTCTAAAAACATAAAGACAAGTGAAGTAACCAAAAACATTGTAGAGGTTTATGGTGCTCCTCATCAAAAAAAGAAAATGGCAGCAGACAATGCAGCCGAGGGTGCATTGTGGTACTTAAAGCATATAGGTTTTGTTTTGAAGAACAAATAA

Protein sequence

>MS.gene86987.t1

MKFQLFHSVICTAEVCLQHYTQYKTWTRAIILLTPPSRVNRTKVYAAKMVKPNLNNQMKMVETVESIVQNPVLKNIDAVQSSVSSSKESVILGKTTTSDEGMKKGCGRSKLYETCAVNHWKPPVFECCKEEGPCHCIMFTFKVIVEMETGKASKNIKTSEVTKNIVEVYGAPHQKKKMAADNAAEGALWYLKHIGFVLKNK