Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88533.t1 | KEH17601.1 | 76.2 | 63 | 15 | 0 | 1 | 63 | 36 | 98 | 1.80E-18 | 101.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88533.t1 | A0A072TK82 | 76.2 | 63 | 15 | 0 | 1 | 63 | 36 | 98 | 1.3e-18 | 101.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049262 | MS.gene88533 | 0.823443 | 1.38E-53 | -1.69E-46 |
| MS.gene049853 | MS.gene88533 | 0.82767 | 1.38E-54 | -1.69E-46 |
| MS.gene049968 | MS.gene88533 | 0.803793 | 2.94E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene88533 | 0.813562 | 2.40E-51 | -1.69E-46 |
| MS.gene050430 | MS.gene88533 | 0.826766 | 2.27E-54 | -1.69E-46 |
| MS.gene052447 | MS.gene88533 | 0.840883 | 6.65E-58 | -1.69E-46 |
| MS.gene05265 | MS.gene88533 | 0.806178 | 9.33E-50 | -1.69E-46 |
| MS.gene05329 | MS.gene88533 | 0.810913 | 9.10E-51 | -1.69E-46 |
| MS.gene053974 | MS.gene88533 | 0.804243 | 2.37E-49 | -1.69E-46 |
| MS.gene054174 | MS.gene88533 | 0.856318 | 3.49E-62 | -1.69E-46 |
| MS.gene054175 | MS.gene88533 | 0.845517 | 3.87E-59 | -1.69E-46 |
| MS.gene055017 | MS.gene88533 | 0.81305 | 3.11E-51 | -1.69E-46 |
| MS.gene055056 | MS.gene88533 | 0.820166 | 7.92E-53 | -1.69E-46 |
| MS.gene055058 | MS.gene88533 | 0.828696 | 7.79E-55 | -1.69E-46 |
| MS.gene05531 | MS.gene88533 | 0.800871 | 1.18E-48 | -1.69E-46 |
| MS.gene055952 | MS.gene88533 | 0.815656 | 8.27E-52 | -1.69E-46 |
| MS.gene058515 | MS.gene88533 | 0.810145 | 1.33E-50 | -1.69E-46 |
| MS.gene059276 | MS.gene88533 | 0.833581 | 4.91E-56 | -1.69E-46 |
| MS.gene059458 | MS.gene88533 | 0.840244 | 9.77E-58 | -1.69E-46 |
| MS.gene059459 | MS.gene88533 | 0.822631 | 2.14E-53 | -1.69E-46 |
| MS.gene059460 | MS.gene88533 | 0.806442 | 8.21E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88533.t1 | MTR_1g037260 | 81.250 | 64 | 12 | 0 | 1 | 64 | 364 | 427 | 2.03e-27 | 102 |
| MS.gene88533.t1 | MTR_0006s0260 | 76.190 | 63 | 15 | 0 | 1 | 63 | 36 | 98 | 2.52e-25 | 92.0 |
| MS.gene88533.t1 | MTR_1g036840 | 73.438 | 64 | 17 | 0 | 1 | 64 | 158 | 221 | 5.06e-25 | 93.6 |
| MS.gene88533.t1 | MTR_1g036780 | 81.579 | 38 | 7 | 0 | 28 | 65 | 60 | 97 | 2.35e-15 | 65.9 |
| MS.gene88533.t1 | MTR_1g037080 | 67.241 | 58 | 16 | 1 | 8 | 65 | 125 | 179 | 1.67e-14 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGAGTGCTGGGTCTGTTCT+TGG | 0.280938 | 1.4:+22391119 | MS.gene88533:CDS |
| AAATGGAAGGGAAGAGGTTA+AGG | 0.305207 | 1.4:+22391009 | None:intergenic |
| TATCGTTGGATACGCGGATT+TGG | 0.316983 | 1.4:+22391058 | MS.gene88533:CDS |
| GTTCTTGGCTGTTTGTTGTT+TGG | 0.338460 | 1.4:+22391134 | MS.gene88533:CDS |
| TCCATTCTCACAGAGCTTCT+CGG | 0.357385 | 1.4:-22391165 | None:intergenic |
| ATCGTTGGATACGCGGATTT+GGG | 0.387066 | 1.4:+22391059 | MS.gene88533:CDS |
| TGGGTAGGTTGGAAGAGTGT+TGG | 0.448290 | 1.4:+22391078 | MS.gene88533:CDS |
| TGGCTGTTTGTTGTTTGGTA+AGG | 0.448429 | 1.4:+22391139 | MS.gene88533:CDS |
| ACCGAGAAGCTCTGTGAGAA+TGG | 0.466087 | 1.4:+22391164 | MS.gene88533:CDS |
| TTCGAAGAAATGCTGAGTGC+TGG | 0.470839 | 1.4:+22391107 | MS.gene88533:CDS |
| TCGAAGAAATGCTGAGTGCT+GGG | 0.503765 | 1.4:+22391108 | MS.gene88533:CDS |
| AATGGAAGGGAAGAGGTTAA+GGG | 0.504713 | 1.4:+22391010 | None:intergenic |
| TGAGAATGGAGAAGTGGAGA+AGG | 0.524457 | 1.4:+22391178 | MS.gene88533:CDS |
| AGAGGGAAATGGAAGGGAAG+AGG | 0.526423 | 1.4:+22391003 | None:intergenic |
| TTGGATACGCGGATTTGGGT+AGG | 0.531236 | 1.4:+22391063 | MS.gene88533:CDS |
| ATACGCGGATTTGGGTAGGT+TGG | 0.549215 | 1.4:+22391067 | MS.gene88533:CDS |
| ACTTATGAATGTGTTATCGT+TGG | 0.553017 | 1.4:+22391044 | MS.gene88533:CDS |
| ATGTGTTATCGTTGGATACG+CGG | 0.561073 | 1.4:+22391052 | MS.gene88533:CDS |
| GCTCTGTGAGAATGGAGAAG+TGG | 0.572425 | 1.4:+22391172 | MS.gene88533:CDS |
| GATTGAAGAGGGAAATGGAA+GGG | 0.639070 | 1.4:+22390997 | None:intergenic |
| GGATTGAAGAGGGAAATGGA+AGG | 0.644339 | 1.4:+22390996 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACTTATTCACATTTGATACA+TGG | + | chr1.4:22391256-22391275 | MS.gene88533:intron | 25.0% |
| !!! | TAATGAGTTTTGTTTTTTGC+AGG | + | chr1.4:22391450-22391469 | MS.gene88533:intron | 25.0% |
| AATCCTTTATCTAACAACAC+AGG | - | chr1.4:22391219-22391238 | None:intergenic | 30.0% | |
| ACTTATGAATGTGTTATCGT+TGG | + | chr1.4:22391044-22391063 | MS.gene88533:CDS | 30.0% | |
| ATCCTTTATCTAACAACACA+GGG | - | chr1.4:22391218-22391237 | None:intergenic | 30.0% | |
| TTCAAGGAGTTGTTAGATAA+AGG | + | chr1.4:22391303-22391322 | MS.gene88533:intron | 30.0% | |
| !!! | AGTTTTTCTGTTTCTACAGT+TGG | + | chr1.4:22391383-22391402 | MS.gene88533:intron | 30.0% |
| ATCAACTTACGTTTATCGTC+TGG | + | chr1.4:22391336-22391355 | MS.gene88533:intron | 35.0% | |
| CATGGTTAATTATGCGAGAA+AGG | + | chr1.4:22391274-22391293 | MS.gene88533:intron | 35.0% | |
| ! | AGACGATAAACGTAAGTTGA+TGG | - | chr1.4:22391337-22391356 | None:intergenic | 35.0% |
| !! | GACGATAAACGTAAGTTGAT+GGG | - | chr1.4:22391336-22391355 | None:intergenic | 35.0% |
| !! | TAAACGTAAGTTGATGGGTT+AGG | - | chr1.4:22391331-22391350 | None:intergenic | 35.0% |
| ATGTGTTATCGTTGGATACG+CGG | + | chr1.4:22391052-22391071 | MS.gene88533:CDS | 40.0% | |
| ! | TGCCCTGTGTTGTTAGATAA+AGG | + | chr1.4:22391213-22391232 | MS.gene88533:intron | 40.0% |
| !! | GTTCTTGGCTGTTTGTTGTT+TGG | + | chr1.4:22391134-22391153 | MS.gene88533:CDS | 40.0% |
| !! | TATCGTCTGGCTTTGAATAG+TGG | + | chr1.4:22391349-22391368 | MS.gene88533:intron | 40.0% |
| !! | TGGCTGTTTGTTGTTTGGTA+AGG | + | chr1.4:22391139-22391158 | MS.gene88533:CDS | 40.0% |
| ATCGTTGGATACGCGGATTT+GGG | + | chr1.4:22391059-22391078 | MS.gene88533:CDS | 45.0% | |
| TATCGTTGGATACGCGGATT+TGG | + | chr1.4:22391058-22391077 | MS.gene88533:CDS | 45.0% | |
| TCCATTCTCACAGAGCTTCT+CGG | - | chr1.4:22391168-22391187 | None:intergenic | 45.0% | |
| TCGAAGAAATGCTGAGTGCT+GGG | + | chr1.4:22391108-22391127 | MS.gene88533:CDS | 45.0% | |
| TGAGAATGGAGAAGTGGAGA+AGG | + | chr1.4:22391178-22391197 | MS.gene88533:CDS | 45.0% | |
| TTCGAAGAAATGCTGAGTGC+TGG | + | chr1.4:22391107-22391126 | MS.gene88533:CDS | 45.0% | |
| ACCGAGAAGCTCTGTGAGAA+TGG | + | chr1.4:22391164-22391183 | MS.gene88533:CDS | 50.0% | |
| ATACGCGGATTTGGGTAGGT+TGG | + | chr1.4:22391067-22391086 | MS.gene88533:CDS | 50.0% | |
| GCGAGAAAGGACGAAGTTCA+AGG | + | chr1.4:22391287-22391306 | MS.gene88533:intron | 50.0% | |
| GCTCTGTGAGAATGGAGAAG+TGG | + | chr1.4:22391172-22391191 | MS.gene88533:CDS | 50.0% | |
| TTGGATACGCGGATTTGGGT+AGG | + | chr1.4:22391063-22391082 | MS.gene88533:CDS | 50.0% | |
| ! | TGGGTAGGTTGGAAGAGTGT+TGG | + | chr1.4:22391078-22391097 | MS.gene88533:CDS | 50.0% |
| !! | CGTCTGGCTTTGAATAGTGG+AGG | + | chr1.4:22391352-22391371 | MS.gene88533:intron | 50.0% |
| !! | CTGAGTGCTGGGTCTGTTCT+TGG | + | chr1.4:22391119-22391138 | MS.gene88533:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 22391011 | 22391480 | 22391011 | ID=MS.gene88533 |
| chr1.4 | mRNA | 22391011 | 22391480 | 22391011 | ID=MS.gene88533.t1;Parent=MS.gene88533 |
| chr1.4 | exon | 22391011 | 22391199 | 22391011 | ID=MS.gene88533.t1.exon1;Parent=MS.gene88533.t1 |
| chr1.4 | CDS | 22391011 | 22391199 | 22391011 | ID=cds.MS.gene88533.t1;Parent=MS.gene88533.t1 |
| chr1.4 | exon | 22391472 | 22391480 | 22391472 | ID=MS.gene88533.t1.exon2;Parent=MS.gene88533.t1 |
| chr1.4 | CDS | 22391472 | 22391480 | 22391472 | ID=cds.MS.gene88533.t1;Parent=MS.gene88533.t1 |
| Gene Sequence |
| Protein sequence |
